CN113943648A - Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity - Google Patents
Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity Download PDFInfo
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- CN113943648A CN113943648A CN202111302462.5A CN202111302462A CN113943648A CN 113943648 A CN113943648 A CN 113943648A CN 202111302462 A CN202111302462 A CN 202111302462A CN 113943648 A CN113943648 A CN 113943648A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 59
- 238000005286 illumination Methods 0.000 title claims abstract description 59
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 55
- 239000007788 liquid Substances 0.000 claims description 60
- 230000000903 blocking effect Effects 0.000 claims description 28
- 238000001125 extrusion Methods 0.000 claims description 28
- 238000003756 stirring Methods 0.000 claims description 23
- 239000011521 glass Substances 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 238000003466 welding Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 4
- 230000001737 promoting effect Effects 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 238000007667 floating Methods 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 12
- 239000001257 hydrogen Substances 0.000 abstract description 12
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract description 10
- 230000001580 bacterial effect Effects 0.000 abstract description 6
- 230000029553 photosynthesis Effects 0.000 abstract description 4
- 238000010672 photosynthesis Methods 0.000 abstract description 4
- 241000233866 Fungi Species 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 15
- 235000015097 nutrients Nutrition 0.000 description 14
- 239000011550 stock solution Substances 0.000 description 9
- 230000033001 locomotion Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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Abstract
The invention relates to the field of bacterial culture, in particular to an anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity, which comprises a supporting underframe, a swinging assembly, a limiting assembly and the like; the supporting chassis is provided with a swinging assembly, and the swinging assembly is provided with a limiting assembly. Light through the illuminator in the lighting fixture provides illumination for photosynthetic bacteria, makes photosynthetic bacteria carry out photosynthesis, and the fungus cell produces hydrogen more, and concave lens frame intermittent type nature downstream makes the illumination scope of illuminator enlarge gradually, makes the illumination that photosynthetic bacteria received more sufficient, realizes the purpose of controlling illumination intensity as required.
Description
Technical Field
The invention relates to the field of bacterial culture, in particular to an anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity.
Background
Photosynthetic bacteria are the earliest prokaryotes on the earth, have an original oxygen-free light energy synthesis system, and the bacterial culture is a technology for artificially growing and reproducing bacteria.
In the process of culturing and managing photosynthetic bacteria, the photosynthetic bacteria need to be managed, the management work comprises daily management operation and test, the common method of manually shaking the culture container enables bacterial cells to float, thallus suspension is kept at regular time, illumination is required to be continuously carried out when the photosynthetic bacteria are cultured, a light source is required to be manually controlled for illumination, and the illuminance is required to be frequently adjusted according to the requirement, so that a great deal of energy is required for culturing the photosynthetic bacteria.
Disclosure of Invention
The invention aims to provide an anti-pollution type photosynthetic bacteria culture device which can replace manual shaking of a culture container to enable precipitated photosynthetic bacteria to float upwards, can add nutrient solution into the culture container, can discharge hydrogen generated by the photosynthetic bacteria and can adjust illumination intensity according to illumination requirements and control the illumination intensity, so as to solve the problems that the conventional method for manually shaking the culture container in daily management operation of photosynthetic bacteria culture needs to enable bacterial cells to float upwards, a light source needs to be manually controlled to illuminate and the illumination intensity needs to be frequently adjusted according to requirements in the background technology.
The technical scheme is as follows: an anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity comprises a supporting bottom frame, a swinging assembly and a limiting assembly, wherein the swinging assembly further comprises a swinging placing frame, a fixed glass frame, a culture dish for culturing photosynthetic bacteria, a first supporting frame, an electric push rod and a slotting frame; the support underframe is provided with a swing assembly which is used for replacing manual shaking of the culture container to play a role in floating the precipitated photosynthetic bacteria;
the swing assembly is provided with a limiting assembly, and the limiting assembly is used for limiting the swing assembly;
the support underframe is rotatably connected with the swing placing frame;
the fixed glass frame is fixedly arranged at the top of the swinging placement frame and is convenient for workers to observe the culture condition of the photosynthetic bacteria;
the culture dish is placed on the swinging placing frame and tightly attached to the fixed glass frame, and a closed space is formed between the culture dish and the fixed glass frame to facilitate the propagation of photosynthetic bacteria;
the first support frame is fixedly arranged on the support underframe;
the first support frame is fixedly provided with an electric push rod;
the slotting machine comprises a slotting frame, wherein the slotting frame is welded at the top end of an electric push rod telescopic shaft, the slotting frame is in spacing fit with a swing placing frame, and the slotting frame is used for pushing the swing placing frame to swing.
Further, spacing subassembly includes spacing and first spring, and the swing is placed the frame and is gone up the symmetry slidingtype and be connected with spacing, spacing and culture dish contact each other, and spacing and swing are placed and are connected with first spring between the frame.
Further, the culture dish rack comprises an exhaust assembly, the swinging placement frame is provided with the exhaust assembly, the exhaust assembly comprises a first exhaust pipe, a support frame, a first sliding support frame, a second spring, a second exhaust pipe, a second sliding support frame, a blocking frame, a second support frame, a first rotating block, a second rotating block and a third spring, the swinging placement frame is provided with the first exhaust pipe, the first exhaust pipe is communicated with the culture dish, one end of the first exhaust pipe is communicated with the support frame, the support frame is connected with the first sliding support frame in a sliding mode, the first sliding support frame is provided with a plurality of air outlet holes, the second spring is connected between the first sliding support frame and the support frame, the support frame is connected with the second exhaust pipe in a connecting mode, the second sliding support frame is connected with the second sliding support frame in a sliding mode, one end of the second sliding support frame is welded with the blocking frame, and the blocking frame is connected with the first sliding support frame in a sliding mode, the first sliding support frame is welded with a second support frame, the second support frame is in mutual contact with the second sliding support frame, the second support frame is rotatably connected with a first rotating block, the second sliding support frame is rotatably connected with a second rotating block, and a third spring is connected between the second rotating block and the first rotating block.
The device further comprises an injection assembly, the swing placement frame is provided with the injection assembly, the injection assembly comprises a liquid guide pipe, a first liquid storage frame, a second liquid storage frame, a perforated plate, a support rod, a blocking plate, a fourth spring, an extrusion frame and a fifth spring, the liquid guide pipe is connected to the swing placement frame in an evenly distributed mode, the liquid guide pipe is communicated with a culture dish, the top end of the liquid guide pipe is communicated with the first liquid storage frame, the top of the first liquid storage frame is fixedly connected with the second liquid storage frame, the second liquid storage frame is fixedly connected with the swing placement frame, the perforated plate is welded in the first liquid storage frame and provided with a liquid outlet, the support rod is symmetrically arranged in the first liquid storage frame, the two support rods are jointly and slidably connected with the blocking plate, the blocking plate is in contact with the perforated plate, the bottom surface of the blocking plate is fixedly connected with the pair of fourth springs, one end of each fourth spring is connected with the first liquid storage frame, and the extrusion frame is connected to the second liquid storage frame in a lifting mode, and a fifth spring is fixedly connected to the extrusion frame, and one end of the fifth spring is connected with the second liquid storage frame.
Further, still including the first subassembly of stirring, be equipped with the first subassembly of stirring on the extrusion frame, the first subassembly of stirring is including slip support bar, slip card post frame and fixed dog, and the slip is connected with the slip support bar on the extrusion frame, and slip support bar bottom surface fixedly connected with slip card post frame, slip card post frame and second slip support frame sliding connection, and the welding of first support frame top has fixed dog.
Further, still including the illumination subassembly, fixed mounting has the illumination subassembly on the frame is placed in the swing, and the illumination subassembly is including third support frame and lighting fixture, and fixed mounting has the third support frame on the frame is placed in the swing, is provided with the lighting fixture on the third support frame.
Further, the device also comprises an illumination intensity control component, the illumination intensity control component is arranged on a third support frame, the illumination intensity control component comprises a third sliding support frame, a sixth spring, a multi-column-clamping frame, a ratchet frame, a first guide frame, a second guide frame, a fixed support frame, a support plate, a pawl, a torsion spring and a concave lens frame, the third support frame is connected with the third sliding support frame in a sliding way, the third sliding support frame is fixedly connected with a pair of sixth springs, one end of each sixth spring is connected with the third support frame, the third sliding support frame is welded with the multi-column-clamping frame, the ratchet frame is welded with the multi-column-clamping frame, the bottom end of the ratchet frame is provided with the first guide frame, the second guide frame is fixedly connected above the ratchet frame, the top surface of the third support frame is fixedly connected with the fixed support frame, the fixed support frame is connected with the support plate in a sliding way, and the support plate is contacted with the first guide frame, the pawl is connected with in the backup pad rotation type, and pawl and ratchet frame contact each other are connected with torsion spring between pawl and the backup pad, are provided with the concave lens frame on the third slip support frame, and third support frame and concave lens frame contact each other, and the lighting fixture is located the concave lens frame.
Further, the assembly is stirred including the second, and the second stock solution frame top is provided with the second and stirs the assembly, and the assembly is stirred including the fluting deflector, stir strip and seventh spring to the second, and the welding of second stock solution frame top has the fluting deflector, and the strip is stirred to sliding connection on the extrusion frame, fluting deflector with stir the spacing cooperation of strip, stir and have linked to the seventh spring on the strip, seventh spring one end and extrusion frame rigid coupling.
The invention has the beneficial effects that:
the frame is placed through control swing and the reciprocating swing of the upper device of the frame, manual shaking of the culture dish is replaced, the photosynthetic bacteria precipitated in the culture dish float upwards, the photosynthetic bacteria can conveniently obtain illumination, and then the good growth of bacteria cells is realized.
The second sliding support frame is blocked through the support frame, the blocking frame does not block the air outlet hole in the first sliding support frame any more, and hydrogen in the support frame can be discharged from the second exhaust pipe through the air outlet hole, so that the photosynthetic bacteria propagation is facilitated.
Extrude the nutrient solution in the second stock solution frame through the extrusion frame, the nutrient solution can promote the jam plate downstream, and the jam plate no longer plugs up the trompil board liquid outlet, and in the nutrient solution flowed into the culture dish through trompil board liquid outlet and catheter, the help photosynthetic bacteria bred.
Light through the illuminator in the lighting fixture provides illumination for photosynthetic bacteria, makes photosynthetic bacteria carry out photosynthesis, and the fungus cell produces hydrogen more, and concave lens frame intermittent type nature downstream makes the illumination scope of illuminator enlarge gradually, makes the illumination that photosynthetic bacteria received more sufficient, realizes the purpose of controlling illumination intensity as required.
Drawings
Fig. 1 is a schematic perspective view of a first embodiment of the present invention.
Fig. 2 is a schematic perspective view of a second embodiment of the present invention.
Fig. 3 is a schematic perspective view of the swing assembly and the limiting assembly of the present invention.
Fig. 4 is a partial perspective view of the swing assembly of the present invention.
Fig. 5 is a schematic perspective view of the spacing assembly of the present invention.
FIG. 6 is a schematic view of a first partial body construction of an exhaust assembly according to the present invention.
Fig. 7 is a schematic cross-sectional perspective view of an exhaust assembly of the present invention.
FIG. 8 is a schematic view of a second partial body construction of an exhaust assembly of the present invention.
Fig. 9 is a schematic perspective view of a portion of the injection assembly of the present invention.
Figure 10 is a schematic view of a first partially cut-away perspective view of the infusion assembly of the present invention.
Figure 11 is a second partially cut-away perspective view of the infusion assembly of the present invention.
Fig. 12 is a schematic perspective view of the illumination assembly of the present invention.
Fig. 13 is a schematic partial perspective view of the illumination intensity control assembly according to the present invention.
FIG. 14 is a schematic perspective view of a second toggle assembly according to the present invention.
Fig. 15 is a schematic perspective view of a second toggle assembly according to the present invention.
Reference numerals: 1_ support chassis, 2_ swing assembly, 21_ swing placement frame, 22_ fixed glass frame, 23_ petri dish, 24_ first support frame, 25_ electric push rod, 26_ slotted frame, 3_ limit assembly, 31_ limit frame, 32_ first spring, 4_ exhaust assembly, 41_ first exhaust pipe, 42_ support frame, 43_ first sliding support frame, 44_ second spring, 45_ second exhaust pipe, 46_ second sliding support frame, 47_ blocking frame, 48_ second support frame, 49_ first rotating block, 410_ second rotating block, 411_ third spring, 5_ injection assembly, 51_ liquid guide pipe, 52_ first liquid storage frame, 53_ second liquid storage frame, 54_ aperture plate, 55_ support rod, 56_ blocking plate, 57_ fourth spring, 58_ extrusion frame, 59_ fifth spring, 6_ first toggle assembly, 61_ sliding support bar, 62_ sliding clamping column frame, 63_ fixed stop, 7_ lighting assembly, 71_ third support frame, 72_ lamp holder, 8_ lighting intensity control assembly, 81_ third sliding support frame, 82_ sixth spring, 83_ multi-clamping column frame, 84_ ratchet frame, 85_ first guide frame, 86_ second guide frame, 87_ fixed support frame, 88_ support plate, 89_ pawl, 810_ torsion spring, 811_ concave lens frame, 9_ second toggle assembly, 91_ slotted guide plate, 92_ toggle bar, 93_ seventh spring.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
An anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity is shown in figures 1-5 and comprises a supporting bottom frame 1, a swinging assembly 2 and a limiting assembly 3, wherein the supporting bottom frame 1 is provided with the swinging assembly 2, and the swinging assembly 2 is provided with the limiting assembly 3 for limiting the swinging assembly 2.
The limiting assembly 3 comprises a limiting frame 31 and a first spring 32, the limiting frame 31 is symmetrically and slidably connected to the swinging placement frame 21, the limiting frame 31 is in mutual contact with the culture dish 23, the limiting frame 31 is used for blocking the culture dish 23, and the first spring 32 is connected between the limiting frame 31 and the swinging placement frame 21.
The staff puts culture dish 23 that is equipped with photosynthetic bacteria and culture solution into swing and places frame 21 in, and culture dish 23 can promote spacing 31 relative motion, makes spacing 31 relative motion and blocks culture dish 23 through the reset action of first spring 32 afterwards. The fixed glass frame 22 can facilitate the staff to observe the culture condition of the photosynthetic bacteria. The electric push rod 25 is manually controlled to stretch by a worker every 6 hours, when the electric push rod 25 stretches, the electric push rod 25 stretches to drive the slotting frame 26 to move upwards, when the electric push rod 25 contracts, the electric push rod 25 contracts to drive the slotting frame 26 to move downwards, the slotting frame 26 reciprocates up and down to drive the swing placing frame 21 and the upper device to swing in a reciprocating mode, manual shaking of the culture dish 23 is replaced, the deposited photosynthetic bacteria in the culture dish 23 float upwards, the photosynthetic bacteria can conveniently obtain sunlight, and accordingly the bacteria cells can grow well.
Example 2
On the basis of embodiment 1, as shown in fig. 3 and 6-9, the hydrogen-discharging culture device further comprises an exhaust assembly 4, the exhaust assembly 4 is arranged on the swing placing frame 21, the exhaust assembly 4 is used for discharging hydrogen, the exhaust assembly 4 comprises a first exhaust pipe 41, a supporting frame 42, a first sliding supporting frame 43, a second spring 44, a second exhaust pipe 45, a second sliding supporting frame 46, a blocking frame 47, a second supporting frame 48, a first rotating block 49, a second rotating block 410 and a third spring 411, the swing placing frame 21 is provided with the first exhaust pipe 41, the first exhaust pipe 41 is communicated with the culture dish 23, one end of the first exhaust pipe 41 is communicated with the supporting frame 42, the supporting frame 42 is connected with the first sliding supporting frame 43 in a sliding manner, the first sliding supporting frame 43 is provided with a plurality of air outlet holes, the second spring 44 is connected between the first sliding supporting frame 43 and the supporting frame 42, the supporting frame 42 is connected with the second exhaust pipe 45, be close to the carriage 42 of first slip support frame 43 on sliding connection have second slip support frame 46, the welding of second slip support frame 46 one end has the jam frame 47, jam frame 47 and first slip support frame 43 sliding connection, jam frame 47 is used for blocking the venthole on the first slip support frame 43, the welding has second support frame 48 on the first slip support frame 43, second support frame 48 and second slip support frame 46 contact each other, the rotation is connected with first turning block 49 on the second support frame 48, the rotation is connected with second turning block 410 on the second slip support frame 46, be connected with third spring 411 between second turning block 410 and the first turning block 49.
When photosynthetic bacteria carries out photosynthesis, photosynthetic bacteria can produce hydrogen, when the inside fungus cell of culture dish 23 produced hydrogen quantity when more, make the culture dish 23 and the increase of pressure in the airtight space of fixed glass frame 22 formation, hydrogen gets into in the carriage 42 through first blast pipe 41, hydrogen can promote first slip support frame 43 and block up frame 47 and move towards keeping away from first blast pipe 41 direction, carriage 42 blocks second slip support frame 46 thereupon, block up the venthole that frame 47 no longer blocked on the first slip support frame 43, hydrogen in the carriage 42 can pass through the venthole and discharge from second blast pipe 45, be favorable to photosynthetic bacteria to breed. After the hydrogen is exhausted, the pressure in the closed space formed by the culture dish 23 and the fixed glass frame 22 is reduced, the stretched second spring 44 is reset to drive the first sliding support frame 43 and the device thereon to move and reset towards the direction close to the first exhaust pipe 41, the blocking frame 47 blocks the air outlet hole on the first sliding support frame 43, and then the first sliding support frame 43 pushes the blocking frame 47 and the second sliding support frame 46 to move and reset towards the direction close to the first exhaust pipe 41.
Example 3
On the basis of embodiment 2, as shown in fig. 9-11, the culture apparatus further includes an injection assembly 5, the swing frame 21 is provided with the injection assembly 5, the injection assembly 5 is used for injecting nutrient solution, the injection assembly 5 includes a liquid guide tube 51, a first liquid storage frame 52, a second liquid storage frame 53, a hole plate 54, a support rod 55, a blocking plate 56, a fourth spring 57, a squeezing frame 58 and a fifth spring 59, the swing frame 21 is connected with the liquid guide tube 51 in a uniformly distributed manner, the liquid guide tube 51 is communicated with the culture dish 23, the top end of the liquid guide tube 51 is communicated with the first liquid storage frame 52, the top of the first liquid storage frame 52 is jointly and fixedly connected with the second liquid storage frame 53 for storing nutrient solution, the second liquid storage frame 53 is fixedly connected with the swing frame 21, the hole plate 54 is welded in the first liquid storage frame 52, the hole plate 54 is provided with a liquid outlet, the support rod 55 is symmetrically arranged in the first liquid storage frame 52, the two supporting rods 55 are jointly connected with a blocking plate 56 in a sliding mode, the blocking plate 56 is in contact with the opening plate 54, the blocking plate 56 is used for blocking a liquid outlet of the opening plate 54, the bottom surface of the blocking plate 56 is fixedly connected with a pair of fourth springs 57, one end of each fourth spring 57 far away from the blocking plate 56 is connected with the first liquid storage frame 52, the second liquid storage frame 53 is connected with a squeezing frame 58 in a lifting mode, the squeezing frame 58 is used for squeezing nutrient solution in the second liquid storage frame 53, the squeezing frame 58 is fixedly connected with a fifth spring 59, and one end of the fifth spring 59 far away from the squeezing frame 58 is connected with the second liquid storage frame 53.
Still including first stirring subassembly 6, be equipped with first stirring subassembly 6 on the extrusion frame 58, first stirring subassembly 6 is including the slip support bar 61, slip card post frame 62 and fixed stop 63, the slip is connected with slip support bar 61 on the extrusion frame 58, slip support bar 61 is used for promoting extrusion frame 58 downstream, slip support bar 61 bottom surface fixedly connected with slip card post frame 62, slip card post frame 62 and second slip support frame 46 sliding connection, first support frame 24 top welding has fixed stop 63, fixed stop 63 is used for blocking slip card post frame 62.
The nutrient solution is stored in second stock solution frame 53, move towards keeping away from first blast pipe 41 direction when second slip support frame 46, and the swing is placed frame 21 and when the device reciprocating swing on it, fixed dog 63 can block slip card column holder 62, slip support bar 61 can promote extrusion frame 58 downstream, extrusion frame 58 extrudees the nutrient solution in the second stock solution frame 53, make the nutrient solution extruded to first stock solution frame 52 in, the nutrient solution can promote jam plate 56 downstream, jam plate 56 no longer plugs up trompil plate 54 liquid outlet, the nutrient solution flows into culture dish 23 through trompil plate 54 liquid outlet and catheter 51, help photosynthetic bacteria breeds. When fixed dog 63 and slip card post frame 62 separation, the fifth spring 59 that compressed resets drives extrusion frame 58 upward movement and resets, and extrusion frame 58 is no longer extrudeed the nutrient solution in the second stock solution frame 53, and the fourth spring 57 that compressed resets drives jam plate 56 upward movement and resets, and jam plate 56 plugs up trompil plate 54 liquid outlet, and the nutrient solution no longer flows to in the catheter 51.
Example 4
On the basis of the embodiment 3, as shown in fig. 12 to 15, the illumination device 7 is further included, the illumination device 7 is fixedly installed on the swing placing frame 21, the illumination device 7 is used for providing illumination for photosynthetic bacteria, the illumination device 7 includes a third supporting frame 71 and a lamp holder 72, the third supporting frame 71 is fixedly installed on the swing placing frame 21, and the lamp holder 72 is arranged on the third supporting frame 71.
The illumination intensity control assembly 8 is arranged on a third support frame 71, the illumination intensity control assembly 8 is used for controlling illumination intensity, the illumination intensity control assembly 8 comprises a third sliding support frame 81, a sixth spring 82, a multi-clamping column frame 83, a ratchet frame 84, a first guide frame 85, a second guide frame 86, a fixed support frame 87, a support plate 88, a pawl 89, a torsion spring 810 and a concave lens frame 811, the third support frame 71 is connected with the third sliding support frame 81 in a sliding mode, the third sliding support frame 81 is fixedly connected with a pair of sixth springs 82, one end of the sixth spring 82 far away from the third sliding support frame 81 is connected with the third support frame 71, the multi-clamping column frame 83 is welded on the third sliding support frame 81, the ratchet frame 84 is welded on the multi-clamping column frame 83, the first guide frame 85 is arranged at the bottom end of the ratchet frame 84, the second guide frame 86 is fixedly connected above the ratchet frame 84 far away from the first guide frame 85, the top surface of the third support frame 71 is fixedly connected with a fixed support frame 87, the fixed support frame 87 is slidably connected with a support plate 88, the support plate 88 is in contact with the first guide frame 85, the first guide frame 85 is used for pushing the support plate 88 and an upper device thereof to move towards the direction close to the third support frame 71, the support plate 88 is rotatably connected with a pawl 89, the pawl 89 is in contact with the ratchet frame 84, the pawl 89 is used for clamping the ratchet frame 84, a torsion spring 810 is connected between the pawl 89 and the support plate 88, the third sliding support frame 81 is provided with a concave lens frame 811, the third support frame 71 is in contact with the concave lens frame 811, and the lamp holder 72 is located in the concave lens frame 811.
The assembly 9 is stirred including the second still, the subassembly 9 is stirred to the second stock solution frame 53 top is provided with the second, the subassembly 9 is stirred including fluting deflector 91 to the second, stir strip 92 and seventh spring 93, the welding of second stock solution frame 53 top has fluting deflector 91, sliding type is connected with on the extrusion frame 58 stirs strip 92, stir strip 92 is used for promoting many kelly framves 83 and the last device downstream, fluting deflector 91 and stir the spacing cooperation of strip 92, it has seventh spring 93 to link to connect on the stir strip 92, keep away from seventh spring 93 one end and the extrusion frame 58 rigid coupling of stir strip 92.
The lamp holder 72 is internally provided with a lighting lamp which provides illumination for the photosynthetic bacteria so that the photosynthetic bacteria can carry out photosynthesis. When the extrusion frame 58 moves downwards, the extrusion frame 58 drives the toggle strip 92 to move downwards, the toggle strip 92 moves towards the direction close to the third support frame 71 under the action of the slotted guide plate 91, the toggle strip 92 continues to move downwards, the toggle strip 92 pushes the multi-clamping-column frame 83 and the upper device thereof to move downwards, and the concave lens frame 811 moves downwards to enlarge the illumination range of the illumination lamp, so that the illumination received by photosynthetic bacteria is more sufficient. Downward movement of the ratchet rack 84 pushes the pawl 89 to toggle, which in turn causes the pawl 89 to catch the ratchet rack 84 through the action of the torsion spring 810.
When the extrusion frame 58 moves upward to reset, the extrusion frame 58 drives the toggle strip 92 to move upward, the toggle strip 92 moves away from the third support frame 71 to reset through the function of the slotted guide plate 91, and the compressed sixth spring 82 cannot reset because the pawl 89 clamps the ratchet rack 84. The more hydrogen is generated by the bacterial cells, the greater the pressure in the closed space formed by the culture dish 23 and the fixed glass frame 22 is, the more the above operation is repeated, the intermittent downward movement of the third sliding support frame 81 and the device thereon is realized, the illumination range is gradually enlarged, and the purpose of controlling the illumination intensity according to the requirement is realized. When the second guiding frame 86 and the supporting plate 88 contact with each other, the second guiding frame 86 pushes the supporting plate 88 and the upper device thereof to move away from the third supporting frame 71, the pawl 89 does not block the ratchet frame 84 any more, the compressed sixth spring 82 resets to drive the third sliding supporting frame 81 and the upper device thereof to move upward, so that the concave lens frame 811 reduces the illumination range of the illumination lamp, then the first guiding frame 85 contacts with the supporting plate 88, the first guiding frame 85 pushes the supporting plate 88 and the upper device thereof to move toward the direction close to the third supporting frame 71, and the pawl 89 blocks the ratchet frame 84 again. After the culture of the photosynthetic bacteria is completed, the worker takes the culture dish 23 out of the swing placing frame 21.
The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Claims (8)
1. An anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity comprises a supporting underframe (1), a swinging assembly (2) and a limiting assembly (3), and is characterized in that the swinging assembly (2) comprises a swinging placing frame (21), a fixed glass frame (22), a culture dish (23) for culturing photosynthetic bacteria, a first supporting frame (24), an electric push rod (25) and a slotting frame (26);
the support chassis (1) is provided with a swing component (2), and the swing component (2) is used for replacing manual shaking of the culture container to play a role in floating the precipitated photosynthetic bacteria;
the swing assembly (2) is provided with a limiting assembly (3), and the limiting assembly (3) is used for limiting the swing assembly (2);
the swinging placing frame (21) is rotatably connected to the supporting underframe (1);
the fixed glass frame (22) is fixedly arranged at the top of the swinging placement frame (21), and the fixed glass frame (22) is convenient for workers to observe the culture condition of photosynthetic bacteria;
the culture dish (23) is placed on the swinging placing frame (21), the culture dish (23) is tightly attached to the fixed glass frame (22), and a closed space is formed between the culture dish (23) and the fixed glass frame (22) to be beneficial to the propagation of photosynthetic bacteria;
the first support frame (24), the first support frame (24) is fixedly arranged on the support chassis (1);
the electric push rod (25) is fixedly arranged on the first support frame (24);
slotted frame (26), electric putter (25) telescopic shaft top welding has slotted frame (26), and frame (21) spacing cooperation is placed with the swing to slotted frame (26), and slotted frame (26) are used for promoting the swing and place the frame (21) swing.
2. The anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity according to claim 1, wherein the limiting component (3) comprises a limiting frame (31) and a first spring (32), the limiting frame (31) is symmetrically and slidably connected to the swinging placement frame (21), the limiting frame (31) and the culture dish (23) are in contact with each other, and the first spring (32) is connected between the limiting frame (31) and the swinging placement frame (21).
3. The anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity according to claim 2, characterized by further comprising an exhaust assembly (4), wherein the exhaust assembly (4) is arranged on the swinging placement frame (21), the exhaust assembly (4) comprises a first exhaust pipe (41), a support frame (42), a first sliding support frame (43), a second spring (44), a second exhaust pipe (45), a second sliding support frame (46), a blocking frame (47), a second support frame (48), a first rotating block (49), a second rotating block (410) and a third spring (411), the swinging placement frame (21) is provided with the first exhaust pipe (41), the first exhaust pipe (41) is communicated with the culture dish (23), one end of the first exhaust pipe (41) is communicated with the support frame (42), and the support frame (42) is slidably connected with the first sliding support frame (43), be provided with a plurality of ventholes on first slip support frame (43), be connected with second spring (44) between first slip support frame (43) and carriage (42), the hookup has second blast pipe (45) on carriage (42), slip connection has second slip support frame (46) on carriage (42), the welding of second slip support frame (46) one end has blockking up frame (47), it has first sliding support frame (43) sliding connection to block up frame (47), the welding has second support frame (48) on first slip support frame (43), second support frame (48) and second sliding support frame (46) each other contact, the rotation is connected with first turning block (49) on second support frame (48), the rotation is connected with second turning block (410) on second slip support frame (46), be connected with third spring (411) between second turning block (410) and first turning block (49).
4. The anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity according to claim 3, further comprising an injection assembly (5), wherein the injection assembly (5) is arranged on the swinging placement frame (21), the injection assembly (5) comprises a liquid guide tube (51), a first liquid storage frame (52), a second liquid storage frame (53), a perforated plate (54), a support rod (55), a blocking plate (56), a fourth spring (57), a squeezing frame (58) and a fifth spring (59), the swinging placement frame (21) is connected with liquid guide tubes (51) in a uniformly distributed manner, the liquid guide tubes (51) are communicated with the culture dish (23), the top ends of the liquid guide tubes (51) are communicated with the first liquid storage frame (52), the top of the first liquid storage frame (52) is fixedly connected with the second liquid storage frame (53), and the second liquid storage frame (53) is fixedly connected with the swinging placement frame (21), an opening plate (54) is welded in the first liquid storage frame (52), a liquid outlet is arranged on the opening plate (54), support rods (55) are symmetrically arranged in the first liquid storage frame (52), the two support rods (55) are jointly connected with a blocking plate (56) in a sliding manner, the blocking plate (56) is mutually contacted with the opening plate (54), the bottom surface of the blocking plate (56) is fixedly connected with a pair of fourth springs (57),
one end of a fourth spring (57) is connected with the first liquid storage frame (52), an extrusion frame (58) is connected to the second liquid storage frame (53) in a lifting mode, a fifth spring (59) is fixedly connected to the extrusion frame (58), and one end of the fifth spring (59) is connected with the second liquid storage frame (53).
5. The anti-pollution photosynthetic bacteria culture device of claim 4, further comprising a first toggle assembly (6), the first toggle assembly (6) is disposed on the extrusion frame (58), the first toggle assembly (6) comprises a sliding support bar (61), a sliding column frame (62) and a fixed stop (63), the sliding support bar (61) is slidably connected on the extrusion frame (58), the sliding column frame (62) is fixedly connected to the bottom surface of the sliding support bar (61), the sliding column frame (62) is slidably connected with the second sliding support frame (46), and the fixed stop (63) is welded above the first support frame (24).
6. The pollution-proof photosynthetic bacteria culture device with controllable illumination intensity according to claim 5, further comprising an illumination assembly (7), wherein the illumination assembly (7) is fixedly installed on the swinging placement frame (21), the illumination assembly (7) comprises a third support frame (71) and a lamp holder (72), the third support frame (71) is fixedly installed on the swinging placement frame (21), and the lamp holder (72) is arranged on the third support frame (71).
7. The pollution-proof photosynthetic bacteria culture device capable of controlling illumination intensity according to claim 6, further comprising an illumination intensity control assembly (8), wherein the illumination intensity control assembly (8) is arranged on the third support frame (71), the illumination intensity control assembly (8) comprises a third sliding support frame (81), a sixth spring (82), a multi-clamping-column frame (83), a ratchet rack (84), a first guide frame (85), a second guide frame (86), a fixed support frame (87), a support plate (88), a pawl (89), a torsion spring (810) and a concave lens frame (811), the third support frame (71) is slidably connected with the third sliding support frame (81), a pair of sixth springs (82) is fixedly connected to the third sliding support frame (81), one end of each sixth spring (82) is connected with the third support frame (71), the multi-clamping-column-frame-type lens holder is characterized in that a multi-clamping-column-frame (83) is welded on a third sliding support frame (81), a ratchet-tooth-frame (84) is welded on the multi-clamping-column-frame (83), a first guide frame (85) is arranged at the bottom end of the ratchet-tooth-frame (84), a second guide frame (86) is fixedly connected above the ratchet-tooth-frame (84), a fixed support frame (87) is fixedly connected to the top surface of the third support frame (71), a support plate (88) is slidably connected onto the fixed support frame (87), the support plate (88) is in contact with the first guide frame (85), a pawl (89) is rotatably connected onto the support plate (88), the pawl (89) is in contact with the ratchet-tooth-frame (84), a torsion spring (810) is connected between the pawl (89) and the support plate (88), a concave lens frame (811) is arranged on the third sliding support frame (81), the third support frame (71) is in contact with the concave lens frame (811), and the lamp holder (72) is located in the concave lens frame (811).
8. The anti-pollution photosynthetic bacteria culture device capable of controlling illumination intensity according to claim 7, further comprising a second stirring assembly (9), wherein the second stirring assembly (9) is arranged at the top of the second liquid storage frame (53), the second stirring assembly (9) comprises a slotted guide plate (91), a stirring bar (92) and a seventh spring (93), the slotted guide plate (91) is welded at the top of the second liquid storage frame (53), the stirring bar (92) is slidably connected on the extrusion frame (58), the slotted guide plate (91) is in limit fit with the stirring bar (92), the seventh spring (93) is connected on the stirring bar (92), and one end of the seventh spring (93) is fixedly connected with the extrusion frame (58).
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| CN202111302462.5A CN113943648A (en) | 2021-11-04 | 2021-11-04 | Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity |
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| CN202111302462.5A CN113943648A (en) | 2021-11-04 | 2021-11-04 | Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2232920Y (en) * | 1995-11-15 | 1996-08-14 | 山西大学 | Device for culture of photosynthesis strain liquid |
| CN103255073A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Method for rapid propagation of high purity photosynthetic bacterium |
| CN108410724A (en) * | 2018-03-26 | 2018-08-17 | 尹康康 | A kind of anaerobism photosynthetic bacteria culture systems |
| CN112625894A (en) * | 2020-12-08 | 2021-04-09 | 郭向东 | Illumination intensity adjusting device is cultivated to microorganism |
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2021
- 2021-11-04 CN CN202111302462.5A patent/CN113943648A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2232920Y (en) * | 1995-11-15 | 1996-08-14 | 山西大学 | Device for culture of photosynthesis strain liquid |
| CN103255073A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Method for rapid propagation of high purity photosynthetic bacterium |
| CN108410724A (en) * | 2018-03-26 | 2018-08-17 | 尹康康 | A kind of anaerobism photosynthetic bacteria culture systems |
| CN112625894A (en) * | 2020-12-08 | 2021-04-09 | 郭向东 | Illumination intensity adjusting device is cultivated to microorganism |
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