CN212199289U

CN212199289U - Cell culture device

Info

Publication number: CN212199289U
Application number: CN201921947992.3U
Authority: CN
Inventors: 田春城
Original assignee: Beijing Expandbiotech Co ltd
Current assignee: Beijing Expandbiotech Co ltd
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2020-12-22
Anticipated expiration: 2029-11-13

Abstract

The utility model relates to a cell culture equipment technical field just discloses a cell culture device, including the incubator, the front of incubator is equipped with the chamber door, and the bottom of incubator inner chamber is equipped with the bottom plate, the top fixed mounting of bottom plate has the rack, the top fixed mounting of incubator one side has the motor. This cell culture device, it rises to drive the support frame through starting the cylinder, thereby it rises to the top of rack in the standing groove to drive the board of placing at support frame top, thereby be convenient for getting of cell culture dish and put, and this device drives the support frame through starting the cylinder and descends, thereby it descends to drive the board of placing at support frame top, and then will place the culture dish of placing on the board and descend to the standing groove in, carry on spacingly to the culture dish, can avoid the culture dish because of rocking the circumstances such as the damage of colliding with each other, when needs add nutrient solution simultaneously, make things convenient for the location and the absorption of sucking disc and culture dish.

Description

Cell culture device

Technical Field

The utility model relates to a cell culture equipment technical field specifically is a cell culture device.

Background

Cell culture refers to a method for simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, the cell culture is an indispensable process for the whole bioengineering technology and one of the biological cloning technologies, the cell culture is the large-scale cloning of the cells, the cell culture technology can be used for culturing a large amount of cells into simple single cells or minimally differentiated multiple cells, which is an indispensable link of the cloning technology, the cell culture is the cloning of the cells, the cell culture technology is an important and common technology in the research method of cell biology, and a large amount of cells can be obtained through cell culture, further, signal transduction of cells, anabolism of cells, growth and proliferation of cells, and the like can be studied.

All adopt the culture dish to carry out cell culture usually when teaching in-process or the preliminary cell culture in laboratory in school, and in the culture process of cell, according to the growth condition of cell, need add the nutrient solution sometimes, however, at current cell device, unable automatic addition nutrient solution needs student or laboratory personnel to hand the liquid-transfering gun and carries out the interpolation of nutrient solution, not only wastes time and energy, and the precision of inefficiency, addition nutrient solution is also lower.

SUMMERY OF THE UTILITY MODEL

The utility model provides a not enough to prior art, the utility model provides a cell culture device, but possess the automatic nutrient solution that adds, efficient advantage, the teaching in-process of school or the laboratory is adopted the culture dish to carry out cell culture usually when preliminary culture cell, and at the culture in-process of cell, according to the growth condition of cell, sometimes need add the nutrient solution, however, at current cell device, unable automatic nutrient solution that adds, need student or laboratory person to hand the liquid-transfering gun and carry out the interpolation of nutrient solution, not only waste time and energy, and inefficiency, the precision of adding the nutrient solution is also lower problem.

The utility model provides a following technical scheme: the utility model provides a cell culture device, includes the incubator, the front of incubator is equipped with the chamber door, and the bottom of incubator inner chamber is equipped with the bottom plate, the top fixed mounting of bottom plate has the rack, the top fixed mounting of incubator one side has the motor, fixed cover is equipped with the lead screw that extends to the incubator inside and cup joint with the activity of incubator inner wall on the output shaft of motor, the top of incubator inner chamber is equipped with the baffle that is located the lead screw below, spacing spout has been seted up at the middle part of baffle, threaded sleeve is equipped with the removal seat on the lead screw, the bottom of baffle is equipped with the ultraviolet lamp, the top of incubator is equipped with the liquid reserve tank, the inside of liquid reserve tank is equipped with the water pump, the liquid end fixed cover.

Preferably, the middle part fixed mounting of rack bottom has the cylinder, the output movable sleeve of cylinder is equipped with the piston rod, the top fixedly connected with support frame of piston rod, the fixed guide bar that is equipped with of both sides of rack inner chamber, the both ends movable sleeve of support frame is on the guide bar of rack inner chamber both sides, and the top fixed mounting of support frame has the board of placing, the standing groove that is used for placing the culture dish is seted up at the top of rack.

Preferably, the size of the placing plate is matched with the size of the placing groove, and the placing plate is movably sleeved with the placing groove.

Preferably, the bottom of the movable seat is fixedly connected with a connecting frame which is movably sleeved with a limiting sliding groove, an air pump is arranged at the bottom of the inner cavity of the connecting frame, an air pipe is fixedly sleeved at the output end of the air pump, the bottom of the connecting frame is fixedly connected with a fixed frame, one side of the top of the fixed frame is fixedly provided with a first hydraulic rod, the output end of the first hydraulic rod extends to the bottom of the fixed frame, and the output end of the first hydraulic rod is movably sleeved with a loop bar, the loop bar is movably sleeved with a spring, and the bottom of the loop bar is fixedly connected with a first connecting plate, the bottom of the first connecting plate is fixedly provided with a sucking disc, the opposite side fixed mounting at fixed bolster top has the second hydraulic stem, the output of second hydraulic stem extends to the bottom and the fixedly connected with second connecting plate of mount, the bottom fixed mounting of second connecting plate has notes liquid pipe.

Preferably, one end of the gas transmission pipe extends to the inside of the first connecting plate and is fixedly sleeved with the top of the inner cavity of the sucking disc.

Preferably, one end of the infusion tube extends into the second connecting plate and is connected with the liquid injection tube.

Compared with the prior art, the utility model discloses possess following beneficial effect:

1. this cell culture device, it rotates to drive the lead screw through starter motor, it is spacing to the link to utilize spacing spout, drive and remove seat horizontal migration, thereby utilize the sucking disc to aim at the culture dish earlier, it drives the sucking disc decline and adsorbs the top of culture dish to start first hydraulic stem, restart the air pump and draw the air between sucking disc and the culture dish top, thereby adsorb the culture dish fastening on the sucking disc, it rises and removes to one side to restart first hydraulic stem and motor drive the top cap of culture dish, and utilize the notes liquid pipe to aim at the culture dish, it drives the notes liquid pipe and descends a take the altitude to restart second hydraulic stem, and start the water pump and inject the nutrient solution in the liquid reserve tank into the culture dish through annotating the liquid pipe, at last again with the culture dish top cap reset lid on the sucking disc on the culture dish, this device can realize the automatic interpolation of cell nutrient solution in the culture dish, degree of automation is high, Convenient to use, efficient, the precision that the nutrient solution added is also higher.

2. This cell culture device, it rises to drive the support frame through starting the cylinder, thereby it rises to the top of rack in the standing groove to drive the board of placing at support frame top, thereby be convenient for getting of cell culture dish and put, and this device drives the support frame through starting the cylinder and descends, thereby it descends to drive the board of placing at support frame top, and then will place the culture dish of placing on the board and descend to the standing groove in, carry on spacingly to the culture dish, can avoid the culture dish because of rocking the circumstances such as the damage of colliding with each other, when needs add nutrient solution simultaneously, make things convenient for the location and the absorption of sucking disc and culture dish.

Drawings

FIG. 1 is a front view of the present invention;

FIG. 2 is a front sectional view of the structure of the present invention;

fig. 3 is a schematic view of the structure sucking disc of the present invention.

In the figure: 1. an incubator; 2. a box door; 3. a base plate; 4. placing a rack; 401. a cylinder; 402. a piston rod; 403. a support frame; 404. a guide bar; 405. placing the plate; 406. a placement groove; 5. a motor; 6. a screw rod; 7. a partition plate; 8. a limiting chute; 9. a movable seat; 901. an air pump; 902. a gas delivery pipe; 903. a fixed mount; 904. a first hydraulic lever; 905. a loop bar; 906. a spring; 907. a first connecting plate; 908. a suction cup; 909. a second hydraulic rod; 910. a second connecting plate; 911. a liquid injection pipe; 912. a connecting frame; 10. an ultraviolet lamp; 11. a liquid storage tank; 12. a water pump; 13. an infusion tube.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, a cell culture apparatus comprises an incubator 1, a chamber door 2 is disposed on the front surface of the incubator 1, a bottom plate 3 is disposed at the bottom of an inner cavity of the incubator 1, a placing rack 4 is fixedly mounted at the top of the bottom plate 3, an air cylinder 401 is fixedly mounted at the middle of the bottom end of the placing rack 4, a piston rod 402 is movably sleeved at the output end of the air cylinder 401, a supporting frame 403 is fixedly connected to the top of the piston rod 402, guide rods 404 are fixedly sleeved at two sides of the inner cavity of the placing rack 4, two ends of the supporting frame 403 are movably sleeved on the guide rods 404 at two sides of the inner cavity of the placing rack 4, a placing plate 405 is fixedly mounted at the top of the supporting frame 403, the size of the placing plate 405 is matched with the size of the placing groove 406, the placing plate 405 is movably sleeved with the placing groove 406, a placing groove 406 for, thereby driving the placing plate 405 on the top of the supporting frame 403 to perform height adjustment in the placing groove 406, thereby facilitating adjustment of the depth of the culture dish placed on the top of the placing plate 405 in the placing groove 406, further facilitating taking and placing of the culture dish on the one hand, improving the safety and stability of the placed culture dish on the other hand, facilitating the addition of the subsequent nutrient solution, the top of one side of the incubator 1 is fixedly provided with the motor 5, the output shaft of the motor 5 is fixedly sleeved with the lead screw 6 extending into the incubator 1 and movably sleeved with the inner wall of the incubator 1, the top of the inner cavity of the incubator 1 is provided with the partition plate 7 positioned below the lead screw 6, the middle part of the partition plate 7 is provided with the limit chute 8, the limit chute 8 arranged on the partition plate 7 is utilized, on the one hand, facilitating horizontal movement of the connecting frame 912, on the other hand, the limit of the connecting frame 912 is utilized by the, the screw 6 is sleeved with a movable base 9 through threads, the bottom of the movable base 9 is fixedly connected with a connecting frame 912, the connecting frame 912 is movably sleeved with a limiting chute 8, an air pump 901 is arranged at the bottom of an inner cavity of the connecting frame 912, the device is used for pumping air between a suction cup 908 and a culture dish top cover by utilizing the air pipe 902 by starting the air pump 901, so that the stability of the suction cup 908 on the culture dish top cover is improved, the air pipe 902 is fixedly sleeved at the output end of the air pump 901, one end of the air pipe 902 extends into the first connecting plate 907 and is fixedly sleeved with the top of the inner cavity of the suction cup 908, the bottom of the connecting frame 912 is fixedly connected with a fixing frame 903, one side of the top of the fixing frame 903 is fixedly provided with a first hydraulic rod 904, the output end of the first hydraulic rod 904 extends to the bottom of the fixing frame 903, a sleeve rod 905 is movably sleeved at, by utilizing the cooperation of the loop bar 905 and the spring 906, the first hydraulic rod 904 is started again to drive the sucker 908 to descend to extrude and adsorb the top cover of the culture dish, the sucker 908 and the top cover of the culture dish are buffered to prevent the top cover of the culture dish from being broken due to rigid extrusion, the bottom of the loop bar 905 is fixedly connected with a first connecting plate 907, the sucker 908 is fixedly arranged at the bottom of the first connecting plate 907, the other side of the top of the fixing frame 903 is fixedly provided with a second hydraulic rod 909, the output end of the second hydraulic rod 909 extends to the bottom of the fixing frame 903 and is fixedly connected with a second connecting plate 910, the bottom of the second connecting plate 910 is fixedly provided with a liquid injection pipe 911, the bottom of the partition plate 7 is provided with an ultraviolet lamp 10, and by utilizing the ultraviolet lamp 10, the ultraviolet sterilization and disinfection can be carried out on the inside of the incubator 1, so as to improve the safety of cell culture in the culture dish and avoid pollution, because the, therefore, a mechanism for adjusting the environment required by cell culture is also arranged in the incubator 1 in the application, the top of the incubator 1 is provided with the liquid storage tank 11, the inside of the liquid storage tank 11 is provided with the water pump 12, the liquid outlet end of the water pump 12 is fixedly sleeved with the infusion tube 13, and one end of the infusion tube 13 extends to the inside of the second connecting plate 910 and is connected with the liquid injection tube 911.

The working principle is as follows: firstly, when a culture dish needs to be placed, a box door 2 on the front side of a culture box 1 is opened, an air cylinder 401 is started to drive a support frame 403 to ascend, so as to drive a placing plate 405 on the top of the support frame 403 to ascend from a placing groove 406 to the top of a placing frame 4, then the cell culture dishes are sequentially placed on a plurality of placing frames 4 on the top of the support frame 403, after the cell culture dishes are placed, the air cylinder 401 is started to drive the support frame 403 to descend, so as to drive the culture dishes on the placing frames 4 to descend to a certain depth in the placing groove 406, finally, when nutrient solution needs to be added, a motor 5 is started to drive a screw rod 6 to rotate, a limiting chute 8 is used for limiting a connecting frame 912, a moving seat 9 is driven to horizontally move, so as to firstly use a suction cup 908 to align to the culture dishes, then a first hydraulic rod 904 is started to drive the suction cup 908 to descend to adsorb the top of, thereby with the absorption of culture dish fastening on sucking disc 908, restart first hydraulic stem 904 and motor 5 drive the top cap of culture dish and rise and remove to one side to make and annotate liquid pipe 911 and aim at the culture dish, restart second hydraulic stem 909 drives and annotates liquid pipe 911 and descend a take the altitude, and start water pump 12 with the nutrient solution in the liquid reserve tank 11 through annotating liquid pipe 911 and pour into the culture dish into, at last again with the culture dish top cap on sucking disc 908 cover on the culture dish that resets, can.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell culture apparatus comprising an incubator (1), characterized in that: the incubator is characterized in that a chamber door (2) is arranged on the front side of the incubator (1), a bottom plate (3) is arranged at the bottom of an inner cavity of the incubator (1), a placing frame (4) is fixedly arranged at the top of the bottom plate (3), a motor (5) is fixedly arranged at the top of one side of the incubator (1), a lead screw (6) which extends into the incubator (1) and is movably sleeved with the inner wall of the incubator (1) is fixedly sleeved on an output shaft of the motor (5), a partition plate (7) positioned below the lead screw (6) is arranged at the top of the inner cavity of the incubator (1), a limiting sliding chute (8) is arranged in the middle of the partition plate (7), a moving seat (9) is sleeved on the lead screw (6) in a threaded manner, an ultraviolet lamp (10) is arranged at the bottom of the partition plate (7), a liquid storage tank (11) is arranged at the top of, an infusion tube (13) is fixedly sleeved at the outlet end of the water pump (12).

2. A cell culture apparatus according to claim 1, wherein: the utility model discloses a culture dish placing device, including rack (4), fixed mounting has cylinder (401) in the middle part of rack (4) bottom, the output movable sleeve of cylinder (401) is equipped with piston rod (402), the top fixedly connected with support frame (403) of piston rod (402), the fixed sleeve in both sides of rack (4) inner chamber is equipped with guide bar (404), the both ends movable sleeve of support frame (403) is on guide bar (404) of rack (4) inner chamber both sides, and the top fixed mounting of support frame (403) has place board (405), standing groove (406) that are used for placing the culture dish are seted up at the top of rack (4).

3. A cell culture assembly according to claim 2, wherein: the size of the placing plate (405) is matched with that of the placing groove (406), and the placing plate (405) is movably sleeved with the placing groove (406).

4. A cell culture apparatus according to claim 1, wherein: the bottom of the movable seat (9) is fixedly connected with a connecting frame (912), the connecting frame (912) is movably sleeved with a limiting sliding groove (8), an air pump (901) is arranged at the bottom of an inner cavity of the connecting frame (912), an air pipe (902) is fixedly sleeved at the output end of the air pump (901), a fixing frame (903) is fixedly connected to the bottom of the connecting frame (912), a first hydraulic rod (904) is fixedly installed at one side of the top of the fixing frame (903), the output end of the first hydraulic rod (904) extends to the bottom of the fixing frame (903), a sleeve rod (905) is movably sleeved at the output end of the first hydraulic rod (904), a spring (906) is movably sleeved on the sleeve rod (905), a first connecting plate (907) is fixedly connected to the bottom of the sleeve rod (905), a sucking disc (908) is fixedly installed at the bottom of the first connecting plate (907), and a second hydraulic rod (909) is fixedly installed at the other side of the top, the output end of the second hydraulic rod (909) extends to the bottom of the fixed frame (903) and is fixedly connected with a second connecting plate (910), and the bottom of the second connecting plate (910) is fixedly provided with a liquid injection pipe (911).

5. A cell culture apparatus according to claim 4, wherein: one end of the air pipe (902) extends to the inside of the first connecting plate (907) and is fixedly sleeved with the top of the inner cavity of the suction cup (908).

6. A cell culture apparatus according to claim 4, wherein: one end of the infusion tube (13) extends into the second connecting plate (910) and is connected with the liquid injection tube (911).