CN210916099U

CN210916099U - Cell continuous culture device for biotechnology

Info

Publication number: CN210916099U
Application number: CN201921791902.6U
Authority: CN
Inventors: 殷华; 贾岳; 魏宁颐; 丁昭莉
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-10-16
Filing date: 2019-10-16
Publication date: 2020-07-03
Anticipated expiration: 2029-10-16

Abstract

The utility model discloses a cell continuous culture device for biotechnology belongs to cell culture equipment field, which comprises a housin, the inside level of casing is equipped with the mounting panel, the inboard of culture tank is equipped with the backup pad parallel with the mounting panel, the piston cylinder is installed on the top of casing. The utility model discloses a gas shunt tubes number, the setting of position and length, make it carry out the air feed to the cell of the different degree of depth in the culture tank simultaneously, and through the contact between gas shunt tubes and the culture medium, make the gas that lets in when the cell of placing in nutrient substance and nutrient substance is bloated the upset, the area of contact between gas and the nutrient substance has also been increased to let in, thereby make can contain more gas that supplies cell growth usefulness among the nutrient substance, use this to provide the air feed condition for the growth of cell, thereby make the culture cell can be comparatively abundant and grow and contact between the required gas.

Description

Cell continuous culture device for biotechnology

Technical Field

The utility model relates to a cell culture equipment field, specificly relate to a cell lasts culture apparatus for biotechnology.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function; at present, most of the existing cell culture devices can not improve the content of gas in nutrient substances, so that cultured cells at the bottom of a culture tank can not be fully contacted with gas required by growth, thereby influencing the normal growth of the cultured cells, and simultaneously, the phenomenon of cell adherence can not be effectively reduced, thereby being not beneficial to ensuring higher cell culture survival rate, so that the development of a cell continuous culture device for biotechnology is urgently needed.

SUMMERY OF THE UTILITY MODEL

1. Technical problem to be solved

An object of the utility model is to provide a cell lasts culture apparatus for biotechnology to solve the existing device that has most cell culture used that has proposed in the above-mentioned background art, because it can not improve the content of the inside gas of nutrient substance, thereby lead to the culture cell of culture tank bottom can not be comparatively abundant and grow required gas between contact, thereby the normal growth of cell has led to the fact the influence to culturing, simultaneously because can not carry out effectual reduction to the phenomenon of cell adherence, and be unfavorable for the problem of the higher cell culture survival rate of guarantee.

2. Technical scheme

In order to solve the above problem, the utility model adopts the following technical scheme:

a cell continuous culture device for biotechnology comprises a shell, wherein a mounting plate is horizontally arranged in the shell, a culture tank with an upward opening is arranged below the mounting plate, the bottom end of the culture tank is connected with a driving motor, a supporting plate parallel to the mounting plate is arranged on the inner side of the culture tank, a piston cylinder is arranged at the top end of the shell, the bottom end of the piston cylinder is connected with a sampling pipe which vertically extends downwards and sequentially penetrates through the mounting plate and the supporting plate, a piston is arranged in the piston cylinder, a discharge hole is formed in one side of the piston cylinder, an air inlet pipeline is fixed on the bottom end surface of the mounting plate through a fixing block, the bottom end of the air inlet pipeline is communicated with a row of gas shunt pipes which vertically extend downwards and penetrate through the supporting plate, the gas shunt pipes are equidistantly distributed in the culture tank, and the lower, the top surface of mounting panel is fixed with the nutrient conveyer pipe through the fixed block, and the bottom of nutrient conveyer pipe is equipped with two nutrient shunt tubes that run through mounting panel and vertical downwardly extending and run through the backup pad, be fixed with four piece at least vertical downwardly and extend to the quartz luminotrons of culture trough lower part in the backup pad, and quartz luminotron equidistance distributes in the inside of culture trough, the equidistance is equipped with four vibration mechanism between the lateral wall of culture trough and the inside wall of casing, and vibration mechanism includes the vibrator through connecting block and casing inside wall fixed connection, the level is fixed in the sleeve of one side of vibrator dorsad connecting block, the level runs through telescopic slide bar, the level is fixed on the vibrator and along the wave spring of slide bar peripheral week side equidistance distribution, with wave spring other end fixed connection's mounting bracket and horizontal mounting in, sleeve and slide bar sliding connection, wave spring's both ends respectively with one side of vibrator connecting block dorsad and one side fixed connection of mounting bracket gyro wheel dorsad, the recess sliding connection that gyro wheel and culture tank outside were seted up, every adjacent two all be equipped with horizontally connect the bracing piece between culture tank lateral wall and casing inside wall between the vibration mechanism, and the one end that the bracing piece was connected the culture tank is equipped with the gyro wheel, the gas outlet that is located the mounting panel below is seted up on the right side of casing.

Preferably, the connecting block is connected the one end surface of casing inside wall and is glued the amortization cotton.

Preferably, the inner surface of the bottom end of the culture tank is formed into a horn-shaped structure with an upward opening.

3. Advantageous effects

The utility model discloses a gas shunt tubes number, the setting of position and length, make it carry out the air feed to the cell of the different degree of depth in the culture tank simultaneously, and through the contact between gas shunt tubes and the culture medium, make the gas that lets in when the cell of placing in nutrient substance and nutrient substance is bloated the upset, the area of contact between gas and the nutrient substance has also been increased to let in, thereby make can contain more gas that supplies cell growth usefulness among the nutrient substance, use this to provide the air feed condition for the growth of cell, thereby make the culture cell can be comparatively abundant and grow and contact between the required gas.

The utility model discloses a setting of quartz luminotron number and position for the cell of cultivateing can be comparatively abundant contact rather than the light that sends, and this provides strong illumination condition for cell growth, thereby greatly reduced because cell illumination is insufficient to the degree of cultivateing cell growth and development in the same phase causes great difference.

The utility model discloses a driving motor's rotation can drive the rotation to the nutrient substance of culture tank inside and the cell of cultivation, thereby make the cell of cultivation can be comparatively abundant contact with nutrient substance, and then for the cultivation of cell provides strong condition, and contact between accessible gyro wheel and the culture tank outer wall, transmit the vibrations that the vibrator produced to the culture tank in, with this to adhering to glutinous cell and shaking down on the culture tank inner wall, with this reduce the influence that the cell adherence caused to cell normal growth, and can continue the vibration that the vibrator produced through wave spring, with this vibrations effect that improves the vibrator, and then greatly reduced the probability that the cell adherence produced.

Drawings

Fig. 1 is a schematic front view of the internal structure of the present invention;

fig. 2 is a schematic view of the top view of the internal structure of the present invention;

fig. 3 is an enlarged schematic view of a portion a in fig. 1.

Reference numerals: 1-shell, 2-mounting plate, 3-culture tank, 4-support plate, 5-sampling tube, 6-piston cylinder, 7-piston, 8-discharge port, 9-gas inlet pipe, 10-gas shunt pipe, 11-nutrient delivery pipe, 12-nutrient shunt pipe, 13-quartz luminotron, 14-vibration mechanism, 15-gas outlet, 16-vibrator, 17-connecting block, 18-silencing cotton, 19-wave spring, 20-sleeve, 21-slide bar, 22-mounting rack, 23-roller and 24-support bar.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and examples.

Examples

As shown in figure 1, a cell continuous culture device for biotechnology, including casing 1, the inside level of casing 1 is equipped with mounting panel 2, and the below of mounting panel 2 is equipped with ascending culture tank 3 of opening, and the bottom of culture tank 3 is connected with driving motor, the bottom internal surface of culture tank 3 is established to the trumpet-shaped structure of opening up, the setting of the trumpet-shaped structure of culture tank 3 bottom surface, make it can guide the cell of its inside cultivation, thereby accelerate the collection speed of cultured cell when sample or ejection of compact, also reduced the degree of adhesion of cultured cell of culture tank 3 internal surface simultaneously, thereby reduced the waste to cultured cell, the inboard of culture tank 3 is equipped with backup pad 4 parallel with mounting panel 2, the top of casing 1 is installed with piston cylinder 6, and the bottom of piston cylinder 6 is connected with vertical downwardly extending and runs through mounting panel 2 in proper order, A sampling tube 5 of a supporting plate 4, a piston 7 is arranged in the piston cylinder 6, a discharge hole 8 is arranged at one side of the piston cylinder 6, an air inlet pipeline 9 is fixed on the bottom surface of the mounting plate 2 through a fixing block, the bottom end of the air inlet pipeline 9 is communicated with a row of gas shunt pipes 10 which vertically extend downwards and penetrate through the supporting plate 4, the gas shunt pipes 10 are equidistantly distributed in the culture tank 3, the lower end of each gas shunt pipe 10 is positioned at different heights, a nutrient delivery pipe 11 is fixed on the top surface of the mounting plate 2 through the fixing block, the bottom end of the nutrient delivery pipe 11 is provided with two nutrient shunt pipes 12 which penetrate through the mounting plate 2, vertically extend downwards and penetrate through the supporting plate 4, at least four quartz luminotrons 13 which vertically extend downwards and reach the lower part of the culture tank 3 are fixed on the supporting plate 4, and the quartz, four vibration mechanisms 14 are equidistantly arranged between the outer side wall of the culture tank 3 and the inner side wall of the shell 1, each vibration mechanism 14 comprises a vibrator 16 which is fixedly connected with the inner side wall of the shell 1 through a connecting block 17, a sleeve 20 which is horizontally fixed on one side of the vibrator 16 back to the connecting block 17, a slide rod 21 which horizontally penetrates through the sleeve 20, a wave spring 19 which is horizontally fixed on the vibrator 16 and is equidistantly distributed along the peripheral circumference of the slide rod 21, a mounting frame 22 which is fixedly connected with the other end of the wave spring 19 and a roller 23 which is horizontally arranged in the mounting frame 22, the sleeve 20 is slidably connected with the slide rod 21, two ends of the wave spring 19 are respectively fixedly connected with one side of the vibrator 16 back to the connecting block 17 and one side of the mounting frame 22 back to the roller 23, silencing cotton 18 is adhered on the surface of one end of the inner side wall of the connecting block 17, which is connected with the shell, reduce the influence of vibrator 16 during operation to casing 1 vibration range with this, also reduced the noise that the device produced at the during operation simultaneously, the recess sliding connection that gyro wheel 23 and culture tank 3 outside were seted up all is equipped with horizontal connection in the bracing piece 24 between culture tank 3 lateral wall and the casing 1 inside wall between every two adjacent vibrating mechanism 14, and the one end that the bracing piece 24 is connected culture tank 3 is equipped with gyro wheel 23, the gas outlet 15 that is located the mounting panel 2 below is seted up on the right side of casing 1.

The specific application process of the continuous cell culture device for biotechnology comprises the following steps: when in use, firstly according to the growth characteristics of the cultured cells, the gas required by the growth of the cultured cells is transmitted into the culture tank 3 through the gas inlet pipeline 9, so as to provide conditions for the growth of the cells, and through the arrangement of the number, the position and the length of the gas shunt pipes 10, the gas can simultaneously supply gas for the cells with different depths in the culture tank 3, and through the contact between the gas shunt pipes 10 and the cultured substances, the introduced gas can bulge and turn the nutrient substances and the cells placed in the nutrient substances, and simultaneously, the contact area between the introduced gas and the nutrient substances is increased, so that the nutrient substances can contain more gas for the growth of the cells, so as to provide conditions for the growth of the cells, and the nutrient substances and the cultured cells in the culture tank 3 can be turned through the rotation of the driving motor, so that the cultured cells can be fully contacted with the nutrient substances, further, powerful conditions are provided for the culture of the cells, the vibration generated by the vibrator 16 can be transmitted into the culture tank 3 through the contact between the roller 23 and the outer wall of the culture tank 3, so that the cells adhered to and adhered to the inner wall of the culture tank 3 are shaken off, the influence of the adherence of the cells on the normal growth of the cells is reduced, the vibration generated by the vibrator 16 can be continued through the wave spring 19, the vibration effect of the vibrator 16 is improved, and the probability of adherence of the cells is greatly reduced;

when the cultured cells need illumination, the cells are provided with illumination through the quartz luminotron 13, and nutrient substances are provided for the cells through the nutrient conveying pipe 11 regularly according to the content of nutrients needed by the growth of the cells, so that the cells can be cultured continuously, the influence of insufficient nutrients on the normal development of the cells due to the long-time growth of the cells is avoided, and then the waste gas generated by the cultured cells is conveyed through the gas outlet 15;

after the cell is cultivateed a period, can pass through the bottom inside that sampling tube 5 transmitted the piston cylinder 6 with the inside cultured cell of culture tank 3 through the pull-up of piston 7 to transmit through discharge gate 8 can, like this alright observe can to the cell that is cultivateing, just so accomplished this cell and lasted culture apparatus's use.

It will be appreciated by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as limitations of the present invention, and that changes and modifications to the above described embodiments will fall within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.

Claims

1. The cell continuous culture device for the biotechnology comprises a shell (1) and is characterized in that a mounting plate (2) is horizontally arranged inside the shell (1), a culture tank (3) with an upward opening is arranged below the mounting plate (2), the bottom end of the culture tank (3) is connected with a driving motor, a support plate (4) parallel to the mounting plate (2) is arranged on the inner side of the culture tank (3), a piston cylinder (6) is installed at the top end of the shell (1), the bottom end of the piston cylinder (6) is connected with a sampling tube (5) which vertically extends downwards and sequentially penetrates through the mounting plate (2) and the support plate (4), a piston (7) is arranged inside the piston cylinder (6), a discharge hole (8) is formed in one side of the piston cylinder (6), and an air inlet pipeline (9) is fixed on the bottom end surface of the mounting plate (2) through a fixing block, and the bottom intercommunication of admission line (9) has a row of vertical downwardly extending and runs through gas shunt tubes (10) of backup pad (4), the equidistance of gas shunt tubes (10) distributes in the inside of culture tank (3), and the lower extreme of every gas shunt tubes (10) all is in not co-altitude, the top surface of mounting panel (2) is fixed with nutrient conveyer pipe (11) through the fixed block, and the bottom of nutrient conveyer pipe (11) is equipped with two nutrient shunt tubes (12) that run through mounting panel (2) and vertical downwardly extending and run through backup pad (4), be fixed with four at least vertical downwardly and extend to quartz luminotrons (13) of culture tank (3) lower part on backup pad (4), and quartz luminotrons (13) equidistance distributes in the inside of culture tank (3), the equidistance is equipped with four vibration mechanism (14) between the lateral wall of culture tank (3) and the inside wall of casing (1), and the vibration mechanism (14) comprises a vibrator (16) fixedly connected with the inner side wall of the shell (1) through a connecting block (17), a sleeve (20) horizontally fixed on one side of the vibrator (16) back to the connecting block (17), a sliding rod (21) horizontally penetrating through the sleeve (20), a wave spring (19) horizontally fixed on the vibrator (16) and distributed along the peripheral side of the sliding rod (21) at equal intervals, a mounting frame (22) fixedly connected with the other end of the wave spring (19) and a roller (23) horizontally installed in the mounting frame (22), the sleeve (20) is in sliding connection with the sliding rod (21), the two ends of the wave spring (19) are respectively fixedly connected with one side of the vibrator (16) back to the connecting block (17) and one side of the mounting frame (22) back to the roller (23), the roller (23) is in sliding connection with a groove formed in the outer side of the culture tank (3), every adjacent two all be equipped with bracing piece (24) between horizontally connect between culture tank (3) lateral wall and casing (1) inside wall between vibration mechanism (14), and the one end that bracing piece (24) connect culture tank (3) is equipped with gyro wheel (23), gas outlet (15) that are located mounting panel (2) below are seted up on the right side of casing (1).

2. The continuous cell culture apparatus for biotechnology according to claim 1, wherein the connecting block (17) is connected to the casing (1) and has a sound-deadening cotton (18) adhered to one end surface of the inner side wall.

3. The continuous cell culture apparatus according to claim 1, wherein the inner surface of the bottom of the culture tank (3) is formed into a horn-like structure with an upward opening.