CN115125138A - Three-dimensional culture apparatus of tumour stem cell - Google Patents
Three-dimensional culture apparatus of tumour stem cell Download PDFInfo
- Publication number
- CN115125138A CN115125138A CN202210864335.2A CN202210864335A CN115125138A CN 115125138 A CN115125138 A CN 115125138A CN 202210864335 A CN202210864335 A CN 202210864335A CN 115125138 A CN115125138 A CN 115125138A
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- box
- bevel gear
- rectangular
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 31
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 31
- 239000001963 growth medium Substances 0.000 claims abstract description 49
- 238000004113 cell culture Methods 0.000 claims abstract description 15
- 230000000007 visual effect Effects 0.000 claims abstract description 10
- 238000005086 pumping Methods 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 15
- 238000009434 installation Methods 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000005740 tumor formation Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- G—PHYSICS
- G08—SIGNALLING
- G08B—SIGNALLING OR CALLING SYSTEMS; ORDER TELEGRAPHS; ALARM SYSTEMS
- G08B7/00—Signalling systems according to more than one of groups G08B3/00 - G08B6/00; Personal calling systems according to more than one of groups G08B3/00 - G08B6/00
- G08B7/06—Signalling systems according to more than one of groups G08B3/00 - G08B6/00; Personal calling systems according to more than one of groups G08B3/00 - G08B6/00 using electric transmission, e.g. involving audible and visible signalling through the use of sound and light sources
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Computer Hardware Design (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a three-dimensional tumor stem cell culture device which comprises an incubator, wherein the top of the incubator is provided with an opening, a placing seat is fixedly connected to the inner wall of the bottom of the incubator, a plurality of placing grooves are formed in the top of the placing seat, culture dishes are placed in the placing grooves and used for culturing tumor stem cells, a case cover is fixed to the top of the incubator in a threaded mode, a storage case is fixedly connected to the top of the case cover, and a culture medium is contained in the storage case. The culture medium feeding device is convenient for the operation of adding culture mediums into a plurality of culture dishes in a sealing manner, avoids pollution caused by opening the box covers, can control the amount of the added culture mediums by the arrangement of the first controller, the flow sensor and the electromagnetic valve, can effectively avoid the phenomenon of adding more or less culture mediums, improves the culture stability, can alarm and remind personnel in time when the culture mediums need to be added by the arrangement of the time control switch and the audible and visual alarm, and is convenient for the personnel to add the culture mediums in time.
Description
Technical Field
The invention relates to the technical field of three-dimensional culture equipment, in particular to a three-dimensional culture device for tumor stem cells.
Background
The tumor cell population is a heterogeneous cell population, wherein only the tumor stem cell subset has the capabilities of unlimited proliferation, self-renewal, multidirectional differentiation and tumor formation and is the root cause of the occurrence, the progression and the relapse of malignant tumors, so the deep research on the special biological characteristics of the tumor stem cells has very important scientific significance, a three-dimensional tumor stem cell culture device is required when the tumor stem cells are cultured, and a culture medium is required to be added for many times when the tumor stem cells are cultured;
traditional three-dimensional culture apparatus of tumour stem cell, when adding the culture medium to the culture dish, need personnel to open the case lid of incubator, just can add the culture medium, open the mode that the incubator adds the culture medium many times, introduce bacterium and debris easily, influence the good growth of tumour stem cell, it does not possess the function of sealed interpolation culture medium, and the volume of adding the culture medium is difficult to handle the accuse, the frequent phenomenon that has personnel to add less or add more, the single adds more or adds less the culture medium, all can influence the growth of tumour stem cell, can not satisfy the user demand, synthesize above-mentioned condition, therefore we have proposed a three-dimensional culture apparatus of tumour stem cell and be used for solving above-mentioned problem.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a three-dimensional tumor stem cell culture device.
The invention provides a three-dimensional tumor stem cell culture device, which comprises an incubator with an opening at the top, wherein a placing seat is fixedly connected to the inner wall of the bottom of the incubator, a plurality of placing grooves for placing culture dishes are formed in the top of the placing seat, the culture dishes are used for culturing tumor stem cells, a case cover is fixed to the top of the incubator in a threaded manner, a storage tank is fixedly connected to the top of the case cover, a culture medium is contained in the storage tank, a material pumping pump is fixedly connected to the top of the case cover, the pumping end of the material pumping pump is communicated and fixed with the bottom of the left side of the storage tank, the material pumping pump is used for pumping the culture medium in the storage tank, a transverse pipe communicated and fixed with the drainage end of the material pumping pump is arranged in the incubator, both ends of the transverse pipe are provided with blocking structures, both sides of the bottom of the transverse pipe are fixedly connected with mounting boxes, and the side and the bottom of the two mounting boxes, which are close to each other, are provided with openings, a rectangular rod is arranged between the two mounting boxes, a guide mechanism is fixedly connected between the front inner wall and the rear inner wall of the left mounting box and fixedly connected with the left end of the rectangular rod, the guide mechanism guides the rectangular rod forwards and backwards, a first thread driving mechanism fixedly connected with the right end of the rectangular rod is arranged between the front inner wall and the rear inner wall of the right mounting box and is used for driving the rectangular rod to move forwards and backwards, a rotary driving mechanism is fixedly sleeved on the first thread driving mechanism and is used for driving the first thread driving mechanism to rotate, a first driving motor is fixedly connected with the bottom of the right mounting box and is fixedly connected with the top end of an output shaft of the first driving motor and the bottom of the rotary driving mechanism, the first driving motor is used for driving the rotary driving mechanism to rotate, a sliding sleeve is sleeved on the rectangular rod in a sliding manner, and a mounting groove is formed in the top of the rectangular rod, a rotary thread driving mechanism is arranged between the inner walls at the two sides of the mounting groove, the right side of the top of the rectangular rod is fixedly connected with a second driving motor, the bottom end of an output shaft of the second driving motor is fixedly connected with the top of the rotary thread driving mechanism, the rotary thread driving mechanism is fixedly connected with the inner wall at the top of the sliding sleeve, and the rotary thread driving mechanism is used for driving the sliding sleeve to move left and right, and the electromagnetic valve can be adjusted in front, back, left and right positions by matching the mounting box, the guide mechanism, the rectangular rod, the first thread driving mechanism, the rotary driving mechanism, the first driving motor, the rotary thread driving mechanism, the second driving motor, the rectangular box, the conical cover, the flow sensor, the first controller, the infrared sensor, the wireless signal receiver, the second controller and the third controller, so that culture media are added into a plurality of culture dishes in the culture box in a sealed manner, and the use flexibility is improved, and the culture medium can be quantitatively added into the culture dish by matching the storage tank, the material pumping pump, the telescopic hose, the quantitative feeding mechanism, the infrared sensor and the wireless signal receiver, so that the phenomenon of adding more or less culture medium can be effectively avoided, the culture stability is improved.
Preferably, guiding mechanism includes the guide bar of fixed connection between left mounting box front side inner wall and rear side inner wall, and the sliding sleeve is equipped with the guide block on the guide bar, the right side of guide block and the left end fixed connection of rectangle pole, and guide block and guide bar cooperate and lead around the rectangle pole.
Preferably, the first thread driving mechanism comprises a first screw, the first screw is rotatably installed between the inner wall of the front side and the inner wall of the rear side of the installation box on the right side, a moving block is sleeved on the first screw in a threaded manner, the left side of the moving block is fixedly connected with the right end of the rectangular rod, and the first screw is matched with the moving block and used for driving the rectangular rod to move back and forth.
Preferably, the rotary driving mechanism comprises a first bevel gear, the first bevel gear is fixedly sleeved on the first screw, the first bevel gear is located on the front side of the moving block, the bottom of the front side of the first bevel gear is meshed with a second bevel gear, the top end of an output shaft of the first driving motor is fixedly connected with the bottom of the second bevel gear, and the first screw can be driven to rotate by the meshing mode of the first bevel gear and the second bevel gear.
Preferably, the rotary thread driving mechanism comprises a second screw rod rotatably installed between the inner walls of the two sides of the installation groove, a rectangular block is sleeved on the second screw rod in a threaded manner, the top of the rectangular block is fixedly connected with the inner wall of the top of the sliding sleeve, a third bevel gear is fixedly sleeved on the second screw rod, a fourth bevel gear is meshed on the right side of the third bevel gear, and the bottom end of an output shaft of the second driving motor is fixedly connected with the top of the fourth bevel gear.
Preferably, the quantitative feeding mechanism comprises a rectangular box fixedly connected to the bottom of the sliding sleeve, the top and the bottom of the rectangular box are both provided with openings, the right side of the rectangular box is communicated and fixed with the left end of the telescopic hose, the bottom of the rectangular box is communicated and fixed with a conical cover, the bottom of the conical cover is communicated and fixed with a flow sensor, the flow sensor is used for detecting the flowing amount of the culture medium, a water outlet of the flow sensor is communicated and fixed with an electromagnetic valve, the bottom of the electromagnetic valve is provided with an outlet, the bottom of the right side of the rectangular box is fixedly connected with a first controller, a material pumping pump, the electromagnetic valve and the flow sensor are electrically connected with the first controller, a plurality of wireless signal receivers are electrically connected with the first controller, an infrared sensor is fixedly arranged on the left side of the rectangular box, and the wireless signal receivers are matched with the infrared sensor to realize the accurate adjustment of the position of the electromagnetic valve, the phenomenon of deviation adjustment of the electromagnetic valve is avoided.
Preferably, the front side of the first driving motor is fixedly and electrically connected with a second controller, the front side of the second driving motor is fixedly and electrically connected with a third controller, the second controller and the third controller are both electrically connected with a plurality of wireless signal receivers, and the second controller, the third controller and the wireless signal receivers are matched with each other to automatically control the first driving motor and the second driving motor to be turned off.
Preferably, the top fixedly connected with audible-visual annunciator of case lid, audible-visual annunciator's left side is fixed and electrically connected with time switch, wherein cooperatees through time switch and audible-visual annunciator, can in time report to the police and remind personnel when needs add the culture medium, makes things convenient for personnel in time to add.
Preferably, the thread groove has all been seted up to the top both sides of incubator, thread groove internal thread cover is equipped with T shape fixing bolt, T shape fixing bolt's top extends to the top of case lid, the case lid thread bush is established on two T shape fixing bolt, the incubator is transparent material, the setting of T shape fixing bolt is convenient for take off the case lid from the incubator to can will violently manage, the mounting box, guiding mechanism, the rectangular rod, first screw thread actuating mechanism, rotary driving mechanism, first driving motor, rotary thread actuating mechanism, second driving motor, sliding sleeve and quantitative reinforced mechanism take out from the incubator.
Preferably, the left side of the rectangular box is fixedly connected with a mounting rod, and the bottom of the mounting rod is fixedly connected with the top of the infrared sensor.
Compared with the prior art, the invention has the beneficial effects that:
1. through the matching of the mounting box, the guide mechanism, the rectangular rod, the first thread driving mechanism, the rotary driving mechanism, the first driving motor, the rotary thread driving mechanism, the second driving motor, the rectangular box, the conical cover, the flow sensor, the first controller, the infrared sensor, the wireless signal receiver, the second controller and the third controller, the front-back left-right position adjustment of the electromagnetic valve can be realized, so that culture media are added into a plurality of culture dishes in the culture box in a sealed mode, the use flexibility is improved, and the phenomenon of external pollution can be effectively avoided by the sealed adding mode;
2. by matching the storage tank, the material pumping pump, the telescopic hose, the rectangular box, the conical cover, the flow sensor, the electromagnetic valve, the first controller, the infrared sensor and the wireless signal receiver, the culture medium can be quantitatively added into the culture dish, the phenomenon of adding more or less culture medium can be effectively avoided, and the culture stability is improved;
3. by matching the time control switch with the audible and visual alarm, the culture medium can be added in time, so that the personnel can be warned in time, and the culture medium can be conveniently added in time by the personnel;
the culture medium feeding device is convenient for the operation of hermetically feeding culture media into a plurality of culture dishes, avoids pollution caused by opening the box covers, can control the amount of the fed culture media by arranging the first controller, the flow sensor and the electromagnetic valve, can effectively avoid the phenomenon of more feeding or less feeding, improves the culture stability, can timely alarm and remind personnel when the culture media need to be fed by arranging the time control switch and the acousto-optic alarm, and is convenient for the personnel to feed in time.
Drawings
FIG. 1 is a schematic structural diagram of a tumor stem cell three-dimensional culture apparatus according to the present invention;
FIG. 2 is a schematic cross-sectional view of FIG. 1;
FIG. 3 is an enlarged schematic view of portion A of FIG. 2;
FIG. 4 is an enlarged schematic view of portion B of FIG. 2;
fig. 5 is a schematic perspective view of a rectangular rod and a sliding sleeve connecting member of a three-dimensional tumor stem cell culture device according to the present invention.
In the figure: 100. an incubator; 101. a placing seat; 102. a culture dish; 103. a box cover; 104. a T-shaped fixing bolt; 1. a storage tank; 2. a material pumping pump; 3. an audible and visual alarm; 4. a transverse tube; 5. a rectangular bar; 6. a wireless signal receiver; 7. an infrared sensor; 8. a flexible hose; 9. mounting a box; 10. a guide rod; 11. a guide block; 12. mounting grooves; 13. a second screw; 14. a sliding sleeve; 15. a rectangular block; 16. a rectangular box; 17. a moving block; 18. mounting a rod; 19. a first controller; 20. a conical cover; 21. a fourth bevel gear; 22. a flow sensor; 23. an electromagnetic valve; 24. a third bevel gear; 25. a second drive motor; 26. a first screw; 27. a first bevel gear; 28. a first drive motor; 29. a second bevel gear; 30. a second controller; 31. and a third controller.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Examples
Referring to fig. 1-5, the embodiment provides a three-dimensional tumor stem cell culture apparatus, which includes an incubator 100 with an open top, a placing seat 101 fixedly connected to an inner wall of a bottom of the incubator 100, a plurality of placing grooves formed on a top of the placing seat 101, a culture dish 102 placed in the placing groove, the culture dish 102 used for culturing tumor stem cells, and a cover 103 threadedly mounted on a top of the incubator 100, wherein thread grooves are formed on both sides of the top of the incubator 100, thread grooves are threadedly mounted with T-shaped fixing bolts 104, tops of the T-shaped fixing bolts 104 extend to an upper side of the cover 103, the cover 103 threadedly mounted on the two T-shaped fixing bolts 104, so that the cover 103 is fixed on the top of the incubator 100 by means of thread fit between the two T-shaped fixing bolts 104 and the corresponding thread grooves, the incubator 100 is made of a transparent material, and the incubator 100 made of a transparent material is convenient for a person to look up inside the incubator 100, the top of the box cover 103 is fixedly connected with a storage box 1, the storage box 1 is filled with culture medium, the top of the box cover 103 is fixedly connected with a material pumping pump 2, the pumping end of the material pumping pump 2 is communicated and fixed with the left bottom of the storage box 1, the material pumping pump 2 is used for pumping the culture medium in the storage box 1, a transverse pipe 4 communicated and fixed with the drainage end of the material pumping pump 2 is arranged in the culture box 100, both ends of the transverse pipe 4 are both arranged into a blocking structure, both sides of the bottom of the transverse pipe 4 are both fixedly connected with mounting boxes 9, the mutually close side and the bottom of the two mounting boxes 9 are both arranged into openings, a rectangular rod 5 is arranged between the two mounting boxes 9, a guide mechanism is fixedly connected between the front inner wall and the rear inner wall of the left mounting box 9, the guide mechanism is fixedly connected with the left end of the rectangular rod 5, wherein the guide mechanism comprises a guide rod 10 fixedly connected between the front inner wall and the rear inner wall of the left mounting box 9, a guide block 11 is slidably sleeved on the guide rod 10, a guide hole which is slidably sleeved with the outer side of the guide rod 10 is formed in the front side of the guide block 11, the right side of the guide block 11 is fixedly connected with the left end of the rectangular rod 5, and the guide block 11 is matched with the guide rod 10 to guide the rectangular rod 5 forwards and backwards;
a first thread driving mechanism fixedly connected with the right end of the rectangular rod 5 is arranged between the front inner wall and the rear inner wall of the right mounting box 9, wherein the first thread driving mechanism comprises a first screw 26, first bearings are fixedly connected on the front inner wall and the rear inner wall of the right mounting box 9, the inner sides of the inner rings of the first bearings are fixedly connected with the outer sides of the first screw 26, so that the first screw 26 is rotatably arranged between the front inner wall and the rear inner wall of the right mounting box 9 through two first bearings, a moving block 17 is sleeved on the first screw 26 in a threaded manner, a second threaded hole is arranged on the front side of the moving block 17 and is in threaded connection with the first screw 26, the effect of driving the moving block 17 to move back and forth through threads can be realized in a manner that the second threaded hole is in threaded connection with the first screw 26, the left side of the moving block 17 is fixedly connected with the right end of the rectangular rod 5, the first screw 26 is matched with the moving block 17 and is used for driving the rectangular rod 5 to move back and forth;
a rotary driving mechanism is fixedly sleeved on the first screw 26, the bottom of the right mounting box 9 is fixedly connected with a first driving motor 28, the rotary driving mechanism comprises a first bevel gear 27, the first bevel gear 27 is fixedly sleeved on the first screw 26, the first bevel gear 27 is positioned on the front side of the moving block 17, the bottom of the front side of the first bevel gear 27 is meshed with a second bevel gear 29, the top end of an output shaft of the first driving motor 28 is fixedly connected with the bottom of the second bevel gear 29, and the first bevel gear 27 and the second bevel gear 29 can be driven to rotate in a meshed mode;
a sliding sleeve 14 is slidably sleeved on the rectangular rod 5, the inner wall of the sliding sleeve 14 is slidably sleeved with the outer side of the rectangular rod 5, the top of the rectangular rod 5 is provided with a mounting groove 12, a rotary thread driving mechanism is installed between the inner walls of two sides of the mounting groove 12, the right side of the top of the rectangular rod 5 is fixedly connected with a second driving motor 25, the rotary thread driving mechanism comprises a second screw 13 rotatably installed between the inner walls of two sides of the mounting groove 12, wherein the inner walls of two sides of the mounting groove 12 are both fixedly connected with a second bearing, the inner side of the inner ring of the second bearing is fixedly connected with the outer side of the second screw 13, the second bearing has the effect of rotatably installing the second screw 13, a rectangular block 15 is threadedly sleeved on the second screw 13, the right side of the rectangular block 15 is provided with a first threaded hole, the first threaded hole is in threaded connection with the second screw 13, and the first threaded hole is in threaded connection with the second screw 13, the effect of driving the rectangular block 15 to move left and right by threads can be realized, the top of the rectangular block 15 is fixedly connected with the inner wall of the top of the sliding sleeve 14, a third bevel gear 24 is fixedly sleeved on the second screw 13, a fourth bevel gear 21 is meshed with the right side of the third bevel gear 24, and the bottom end of an output shaft of a second driving motor 25 is fixedly connected with the top of the fourth bevel gear 21;
the bottom of the sliding sleeve 14 is fixedly connected with a quantitative feeding mechanism, wherein the quantitative feeding mechanism comprises a rectangular box 16 fixedly connected to the bottom of the sliding sleeve 14, the top and the bottom of the rectangular box 16 are both provided with openings, the bottom of the rectangular box 16 is communicated and fixed with a conical cover 20, the bottom of the conical cover 20 is communicated and fixed with a flow sensor 22, the flow sensor 22 is used for detecting the flowing amount of a culture medium, a water outlet of the flow sensor 22 is communicated and fixed with an electromagnetic valve 23, the bottom of the electromagnetic valve 23 is provided with an outlet, the bottom of the right side of the rectangular box 16 is fixedly connected with a first controller 19, the pumping pump 2, the electromagnetic valve 23 and the flow sensor 22 are all electrically connected with the first controller 19, and the first controller 19, the pumping pump 2, the electromagnetic valve 23 and the flow sensor 22 are matched with each other to effectively control the amount of the added culture medium;
the right side of the rectangular box 16 is communicated with the bottom of the transverse pipe 4 and is fixedly provided with the same telescopic hose 8, the telescopic hose 8 is positioned at the rear side of the rectangular rod 5, the left side of the rectangular box 16 is fixedly connected with an installation rod 18, the bottom of the installation rod 18 is fixedly connected with an infrared sensor 7, the left side of the top of the culture dish 102 is fixedly provided with a wireless signal receiver 6 matched with the infrared sensor 7, the wireless signal receiver 6 is electrically connected with a first controller 19, a first driving motor 28 and a second driving motor 25, the front side of the first driving motor 28 is fixedly and electrically connected with a second controller 30, the front side of the second driving motor 25 is fixedly and electrically connected with a third controller 31, the second controller 30 and the third controller 31 are both electrically connected with a plurality of wireless signal receivers 6, the second controller 30 and the third controller 31 are matched with the wireless signal receivers 6, can automatically control the closing of the first driving motor 28 and the second driving motor 25, the top of the box cover 103 is fixedly connected with the audible and visual alarm 3, the left side of the audible and visual alarm 3 is fixedly and electrically connected with a time control switch, wherein, the time control switch is matched with the audible and visual alarm 3, so that the culture medium can be timely alarmed to remind personnel when the culture medium needs to be added, the culture medium can be conveniently added by the personnel in time, the culture medium can be conveniently added into a plurality of culture dishes in a sealing way, the pollution caused by opening the box cover 103 is avoided, the arrangement of the first controller 19, the flow sensor 22 and the electromagnetic valve 23 can realize the control of the amount of the added culture medium, can effectually avoid adding more or the phenomenon of adding less, improve and cultivate stability, and through time switch and audible-visual annunciator 3's setting, can in time report to the police and remind personnel when needs add the culture medium, make things convenient for personnel in time to add.
In the embodiment, the time for adding the culture medium is set through the time control switch, when the time for adding the culture medium is reached, the time control switch controls the audible and visual alarm 3 to start to emit audible and visual alarm to remind people, so that the people can conveniently add the culture medium in time, the people can observe the position of the electromagnetic valve 23 through the incubator 100, when the position of the electromagnetic valve 23 is staggered with the position of the culture dish 102 to be added, the position of the electromagnetic valve 23 needs to be adjusted, the second driving motor 25 is started in the forward direction, the output shaft of the second driving motor 25 drives the fourth bevel gear 21 to rotate, the fourth bevel gear 21 drives the third bevel gear 24 meshed with the fourth bevel gear to rotate, the third bevel gear 24 drives the second screw rod 13 to rotate, under the action of the first threaded hole formed in the rectangular block 15, the second screw rod 13 rotates to drive the rectangular block 15 to move rightwards, the rectangular block 15 drives the sliding sleeve 14 to slide on the rectangular rod 5, the sliding sleeve 14 drives the rectangular box 16 to move rightwards, the rectangular box 16 drives the electromagnetic valve 23 to move rightwards sequentially through the conical cover 20 and the flow sensor 22, the rectangular box 16 drives the infrared sensor 7 to move rightwards through the mounting rod 18, when the infrared sensor 7 is aligned with the corresponding wireless signal receiver 6, the electromagnetic valve 23 is located above the corresponding culture dish 102 at the moment, the infrared sensor 7 sends infrared signals to the wireless signal receiver 6, the wireless signal receiver 6 transmits the signals to the first controller 19 and the third controller 31 when receiving the signals, the third controller 31 controls the second driving motor 25 to be closed, meanwhile, the first controller 19 controls the flow sensor 22, the pumping pump 2 and the electromagnetic valve 23 to be opened, the pumping pump 2 pumps the culture medium in the storage box 1, and the pumped culture medium enters the rectangular box 16 sequentially through the transverse pipe 4 and the telescopic hose 8, the culture medium in the rectangular box 16 sequentially flows into the corresponding culture dish 102 through the conical cover 20, the flow sensor 22 and the electromagnetic valve 23, the flow sensor 22 detects the outflow amount, when the flow sensor 22 detects that the outflow amount of the culture medium reaches a preset value, the flow sensor 22 transmits a signal to the first controller 19, the first controller 19 controls the electromagnetic valve 23 and the material pumping pump 2 to be closed, so that the operation of adding the culture medium in a sealing manner is realized, the pollution caused by opening the box cover 103 is avoided, and the arrangement of the first controller 19, the flow sensor 22 and the electromagnetic valve 23 can control the amount of the added culture medium, the phenomenon of adding more or less culture medium can be effectively avoided, and the culture stability is improved;
when a culture medium needs to be added into the culture dish 102 on the rear side, the first driving motor 28 can be started in the forward direction, the output shaft of the first driving motor 28 drives the second bevel gear 29 to rotate, the second bevel gear 29 drives the first bevel gear 27 meshed with the second bevel gear to rotate, the first bevel gear 27 drives the first screw 26 to rotate, under the action of a second threaded hole formed in the moving block 17, the first screw 26 rotates to drive the moving block 17 to move backwards, the moving block 17 drives the rectangular rod 5 to move backwards, the rectangular rod 5 drives the guide block 11 to slide backwards on the guide rod 10, the rectangular rod 5 sequentially drives the electromagnetic valve 23 to move backwards through the sliding sleeve 14, the rectangular box 16, the conical cover 20 and the flow sensor 22, when the electromagnetic valve 23 moves backwards to be aligned with the corresponding culture dish 102, the rectangular box 16 drives the infrared sensor 7 to be aligned with the corresponding wireless signal receiver 6 through the mounting rod 18, the wireless signal receiver 6 transmits signals to the first controller 19 and the second controller 30, the second controller 30 controls the first driving motor 28 to be turned off, and the first controller 19 controls the flow sensor 22, the electromagnetic valve 23 and the pumping pump 2 to be turned on, so that the operation of adding the culture medium can be performed again, and the culture medium can be added into the plurality of culture dishes 102 in the incubator 100 by adjusting the position of the electromagnetic valve 23 in a front-back, left-right and left-right manner, so that the use flexibility is improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (10)
1. The utility model provides a three-dimensional culture apparatus of tumour stem cell, includes incubator (100) that the top is fixed with case lid (103), placing seat (101) of a plurality of standing grooves are seted up at fixedly connected with a top on the bottom inner wall of incubator (100), culture dish (102) have been placed in the standing groove, a serial communication port, storage case (1) of splendid attire culture medium is equipped with to top fixedly connected with of case lid (103), material pump (2) that top fixedly connected with of case lid (103) one and bin (1) are inside to be linked together, be equipped with in incubator (100) with material pump (2) drainage end intercommunication and fixed violently manage (4), the both ends of violently managing (4) are all established to the block structure, the equal fixedly connected with mounting box (9) in bottom both sides of violently managing (4), be equipped with rectangle pole (5) between two mounting boxes (9), fixedly connected with one and rectangle pole (5) left side fixed connection between the front side inner wall and the rear side inner wall of left side of left mounting box (9) A connected guide mechanism, a first thread driving mechanism fixedly connected with the right end of the rectangular rod (5) is installed between the front inner wall and the rear inner wall of the right mounting box (9), a rotary driving mechanism is fixedly sleeved on the first thread driving mechanism, a first driving motor (28) installed with the rotary driving mechanism is fixedly connected with the bottom of the right mounting box (9), a sliding sleeve (14) is sleeved on the rectangular rod (5) in a sliding manner, a mounting groove (12) is formed in the top of the rectangular rod (5), the rotary thread driving mechanism is installed between the inner walls on the two sides of the mounting groove (12), a second driving motor (25) installed with the rotary thread driving mechanism is fixedly connected with the right side of the top of the rectangular rod (5), the rotary thread driving mechanism is fixedly connected with the inner wall on the top of the sliding sleeve (14), and a quantitative feeding mechanism is fixedly connected with the bottom of the sliding sleeve (14), the quantitative feeding mechanism is communicated with the bottom of the transverse pipe (4) and is fixed with the same telescopic hose (8), the infrared sensor (7) is fixedly mounted on the left side of the quantitative feeding mechanism, the wireless signal receiver (6) matched with the infrared sensor (7) is fixedly mounted on the left side of the top of the culture dish (102), and the wireless signal receiver (6) is electrically connected with the quantitative feeding mechanism, the first driving motor (28) and the second driving motor (25).
2. The three-dimensional tumor stem cell culture device according to claim 1, wherein the guide mechanism comprises a guide rod (10) fixedly connected between the front inner wall and the rear inner wall of the left mounting box (9), a guide block (11) is slidably sleeved on the guide rod (10), and the right side of the guide block (11) is fixedly connected with the left end of the rectangular rod (5).
3. The three-dimensional tumor stem cell culture device according to claim 1, wherein the first screw driving mechanism comprises a first screw (26), the first screw (26) is rotatably installed between the front inner wall and the rear inner wall of the right mounting box (9), a moving block (17) is sleeved on the first screw (26) in a threaded manner, and the left side of the moving block (17) is fixedly connected with the right end of the rectangular rod (5).
4. The three-dimensional tumor stem cell culture device according to claim 3, wherein the rotary driving mechanism comprises a first bevel gear (27), the first bevel gear (27) is fixedly sleeved on the first screw (26), the first bevel gear (27) is located at the front side of the moving block (17), the bottom of the front side of the first bevel gear (27) is engaged with a second bevel gear (29), and the top end of the output shaft of the first driving motor (28) is fixedly connected with the bottom of the second bevel gear (29).
5. The three-dimensional tumor stem cell culture device according to claim 1, wherein the rotary screw driving mechanism comprises a second screw (13) rotatably installed between the inner walls of the two sides of the installation groove (12), a rectangular block (15) is threadedly sleeved on the second screw (13), the top of the rectangular block (15) is fixedly connected with the inner wall of the top of the sliding sleeve (14), a third bevel gear (24) is fixedly sleeved on the second screw (13), a fourth bevel gear (21) is meshed on the right side of the third bevel gear (24), and the bottom end of the output shaft of the second driving motor (25) is fixedly connected with the top of the fourth bevel gear (21).
6. The three-dimensional tumor stem cell culture device according to claim 1, wherein the quantitative feeding mechanism comprises a rectangular box (16) fixedly connected to the bottom of a sliding sleeve (14), the top and the bottom of the rectangular box (16) are both provided with openings, the right side of the rectangular box (16) is fixedly connected to the left end of the flexible hose (8), the bottom of the rectangular box (16) is fixedly connected to a conical cover (20), the bottom of the conical cover (20) is fixedly connected to a flow sensor (22), the water outlet of the flow sensor (22) is fixedly connected to a solenoid valve (23), the bottom of the solenoid valve (23) is provided with an outlet, the right bottom of the rectangular box (16) is fixedly connected to a first controller (19), the pumping pump (2), the solenoid valve (23) and the flow sensor (22) are electrically connected to the first controller (19), and the plurality of wireless signal receivers (6) are electrically connected to the first controller (19), the infrared sensor (7) is fixedly arranged on the left side of the rectangular box (16).
7. The three-dimensional tumor stem cell culture device according to claim 1, wherein a second controller (30) is fixed to and electrically connected to the front side of the first driving motor (28), a third controller (31) is fixed to and electrically connected to the front side of the second driving motor (25), and both the second controller (30) and the third controller (31) are electrically connected to the plurality of wireless signal receivers (6).
8. The three-dimensional tumor stem cell culture device according to claim 1, wherein an audible and visual alarm (3) is fixedly connected to the top of the box cover (103), and a time control switch is fixedly and electrically connected to the left side of the audible and visual alarm (3).
9. The three-dimensional tumor stem cell culture device according to claim 1, wherein both sides of the top of the culture box (100) are provided with thread grooves, the thread grooves are internally threaded and sleeved with T-shaped fixing bolts (104), the tops of the T-shaped fixing bolts (104) extend to the upper side of the box cover (103), the box cover (103) is threaded and sleeved on the two T-shaped fixing bolts (104), and the culture box (100) is made of transparent material.
10. The three-dimensional tumor stem cell culture device according to claim 6, wherein a mounting rod (18) is fixedly connected to the left side of the rectangular box (16), and the bottom of the mounting rod (18) is fixedly connected to the top of the infrared sensor (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210864335.2A CN115125138A (en) | 2022-07-21 | 2022-07-21 | Three-dimensional culture apparatus of tumour stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210864335.2A CN115125138A (en) | 2022-07-21 | 2022-07-21 | Three-dimensional culture apparatus of tumour stem cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115125138A true CN115125138A (en) | 2022-09-30 |
Family
ID=83384831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210864335.2A Pending CN115125138A (en) | 2022-07-21 | 2022-07-21 | Three-dimensional culture apparatus of tumour stem cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115125138A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108865884A (en) * | 2018-06-27 | 2018-11-23 | 深圳市安思科电子科技有限公司 | A kind of cell culture platform with accurate quantification charging function |
| CN111420135A (en) * | 2020-04-09 | 2020-07-17 | 西安交通大学医学院第一附属医院 | Improved multifunctional drainage device special for thoracic surgery |
| CN212199289U (en) * | 2019-11-13 | 2020-12-22 | 北京易科拜德科技有限公司 | Cell culture device |
| CN215517475U (en) * | 2021-08-30 | 2022-01-14 | 上海华颜医药科技有限公司 | Stem cell culture device is used in preparation of anti-aging reagent |
| CN216192396U (en) * | 2021-10-27 | 2022-04-05 | 天津赛尔源生物科技有限公司 | Automatic nutrition adding mechanism for cell culture |
| CN216337718U (en) * | 2021-12-02 | 2022-04-19 | 宁楠 | A culture apparatus for biological cell |
-
2022
- 2022-07-21 CN CN202210864335.2A patent/CN115125138A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108865884A (en) * | 2018-06-27 | 2018-11-23 | 深圳市安思科电子科技有限公司 | A kind of cell culture platform with accurate quantification charging function |
| CN212199289U (en) * | 2019-11-13 | 2020-12-22 | 北京易科拜德科技有限公司 | Cell culture device |
| CN111420135A (en) * | 2020-04-09 | 2020-07-17 | 西安交通大学医学院第一附属医院 | Improved multifunctional drainage device special for thoracic surgery |
| CN215517475U (en) * | 2021-08-30 | 2022-01-14 | 上海华颜医药科技有限公司 | Stem cell culture device is used in preparation of anti-aging reagent |
| CN216192396U (en) * | 2021-10-27 | 2022-04-05 | 天津赛尔源生物科技有限公司 | Automatic nutrition adding mechanism for cell culture |
| CN216337718U (en) * | 2021-12-02 | 2022-04-19 | 宁楠 | A culture apparatus for biological cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110073954B (en) | Retractable antiskid plant watering device that falls for household balcony | |
| CN115125138A (en) | Three-dimensional culture apparatus of tumour stem cell | |
| CN218931052U (en) | Pneumatic vacuum gate valve casing | |
| CN217722043U (en) | Water and fertilizer integrated irrigation and fertilization machine | |
| CN214282172U (en) | Municipal works are with green irrigation equipment that plants | |
| CN214709678U (en) | Fish tank with automatic feeding function | |
| CN112715468A (en) | Modern agriculture breeds pond and uses oxygenation device | |
| CN109275631B (en) | Intelligent fishery device | |
| CN111109182A (en) | Fish tank capable of automatically cleaning and changing water | |
| CN112413153A (en) | Anti-blocking valve | |
| CN215160971U (en) | Wine receiving device of wine making production line | |
| CN216979070U (en) | Soil fumigation test device | |
| CN207083796U (en) | A kind of livestock breed aquatics automatic water-supply charging gear | |
| CN212896633U (en) | Dual-purpose device for faucet | |
| CN221025430U (en) | Chemical storage tank convenient to take polyethylene glycol tetra-polyricinoleate | |
| CN215123338U (en) | Aquaculture is with directional material device of throwing | |
| CN218337151U (en) | Liquid manure integration mixing watering device | |
| CN219019953U (en) | Probiotics feeder for improving physique of aquatic animals | |
| CN214629200U (en) | Black swan breeds with aseptic isolating device | |
| CN209555240U (en) | A kind of automatic feeding system for cultivation bucket | |
| CN214206716U (en) | A plant frame for soilless culture vegetables | |
| CN216186050U (en) | Realize automatic water sampling's environmental protection unmanned aerial vehicle | |
| CN217171454U (en) | Water storage bucket that regulation type pure water equipment used | |
| CN219502647U (en) | Quantitative feeding device | |
| CN114568161B (en) | Plant display device for interior decoration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |