CN215517475U - Stem cell culture device is used in preparation of anti-aging reagent - Google Patents

Stem cell culture device is used in preparation of anti-aging reagent Download PDF

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Publication number
CN215517475U
CN215517475U CN202122066569.6U CN202122066569U CN215517475U CN 215517475 U CN215517475 U CN 215517475U CN 202122066569 U CN202122066569 U CN 202122066569U CN 215517475 U CN215517475 U CN 215517475U
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China
Prior art keywords
cell culture
main body
culture
culture solution
stem cell
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Expired - Fee Related
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CN202122066569.6U
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Chinese (zh)
Inventor
江晔
程志强
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Shanghai Huayan Medicine Technology Co ltd
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Shanghai Huayan Medicine Technology Co ltd
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Priority to CN202122066569.6U priority Critical patent/CN215517475U/en
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Abstract

The utility model discloses a stem cell culture device for preparing an anti-aging reagent, which comprises a cell culture box main body, wherein a rotatable bearing platform is arranged in an inner cavity of the cell culture box main body, a culture dish is arranged at the top end of the bearing platform, a culture solution adding mechanism is arranged at the top end of the cell culture box main body, the culture solution adding mechanism comprises a culture solution box, a discharge pipe is fixedly communicated with a discharge hole at the bottom end of the culture solution box, the outer wall of the discharge pipe penetrates through the cell culture box main body and extends to the inner cavity, a flow meter and an electromagnetic valve are fixedly arranged at the bottom of the discharge pipe, and the electromagnetic valve is positioned below the flow meter. According to the utility model, the culture solution box, the discharge pipe, the flow meter and the electromagnetic valve are arranged on the cell culture box main body, so that a certain amount of culture solution can be added into the culture dish when the box door is not required to be opened, pollution caused by contact of stem cells with the outside when the culture solution is added is avoided, and the quality of stem cell culture is improved.

Description

Stem cell culture device is used in preparation of anti-aging reagent
Technical Field
The utility model relates to the field of cell culture devices, in particular to a stem cell culture device for preparing an anti-aging reagent.
Background
Stem cells are a type of pluripotent cells with self-replicating ability, which can be differentiated into various functional cells under certain conditions, and are classified into three types according to the developmental potential of the stem cells: the stem cell is an insufficiently differentiated and immature cell, has potential functions of regenerating various tissues and organs and human bodies, and is called as a universal cell in the medical field.
At present, stem cells are cultured by putting a culture dish filled with the stem cells into a cell culture box, but the stem cells cultured in the cell culture box need to be added with culture solution regularly to meet the culture of the stem cells, and the culture solution is added into the cell culture box by an operator by opening a box door of the cell culture box, so that the culture solution is manually added into the culture dish, and the cell culture box filled with the stem cells is opened frequently, so that the cultured stem cells are easily polluted, and the quality of the culture of the stem cells is reduced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a stem cell culture device for preparing an anti-aging reagent so as to solve the problems in the background technology.
In order to achieve the purpose, the utility model provides the following technical scheme: a stem cell culture device for preparing an anti-aging reagent comprises a cell culture box main body, wherein a rotatable bearing platform is arranged in an inner cavity of the cell culture box main body, a culture dish is arranged at the top end of the bearing platform, and a culture solution adding mechanism is arranged at the top end of the cell culture box main body;
culture solution adds mechanism includes the culture solution case, the fixed intercommunication of discharge gate of culture solution bottom of the case portion has the discharging pipe, just the outer wall of discharging pipe runs through cell culture case main part and extends to the inner chamber, the bottom fixed mounting of discharging pipe has flowmeter and solenoid valve, just the solenoid valve is located flowmeter below position.
Preferably, a material guiding hose is fixedly communicated with a discharge port of the electromagnetic valve, and the outer wall of the material guiding hose is arc-shaped.
Preferably, the front of the cell incubator main body is hinged with a chamber door, the top of the chamber door is provided with a transparent glass window, and the top end of the cell incubator main body is provided with an operation touch screen.
Preferably, the bottom mounting of cell culture case main part inner chamber is provided with the reduction gear, the axle center department of plummer bottom is connected with the output transmission of reduction gear, just the outer wall of cell culture case main part is provided with the driving piece, the driving piece is connected with the input transmission of reduction gear.
Preferably, the reduction gear is including bearing cover, worm wheel, worm and drive shaft, the worm wheel is fixed to be cup jointed in the outer wall of drive shaft, the outer wall of worm is connected with the outer wall meshing of worm wheel, the top of drive shaft run through and bear the cover and with plummer bottom axle center department fixed connection.
Preferably, the culture dish is a plurality of, and is a plurality of the culture dish is the avris that the annular interval set up in the plummer top.
Preferably, the corners of the bottom end of the cell culture box main body are fixedly connected with feet, and the bottom ends of the four feet are fixedly connected with rubber pads.
The utility model has the technical effects and advantages that:
(1) according to the utility model, the culture solution box, the discharge pipe, the flow meter and the electromagnetic valve are arranged on the cell culture box main body, so that a certain amount of culture solution can be added into the culture dish when the box door is not required to be opened, and the pollution caused by the contact of stem cells with the outside when the culture solution is added is avoided, thereby improving the quality of stem cell culture;
(2) the cell culture box can drive the bearing platform to rotate by utilizing the bearing platform, the speed reducer and the driving piece which are arranged at the bottom of the cell culture box, and is matched with the culture dishes which are arranged on the bearing platform at intervals in a ring shape, so that the adding operation of quantitative culture solution in the culture dishes is realized in sequence, and the culture efficiency of stem cells is improved.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a front sectional structural view of the present invention.
Fig. 3 is a front sectional view of the material guiding hose according to the present invention.
FIG. 4 is a schematic sectional view of the decelerator according to the present invention.
In the figure: 1. a cell incubator body; 2. a bearing table; 3. a culture dish; 4. a culture solution box; 5. a discharge pipe; 6. a flow meter; 7. an electromagnetic valve; 8. a material guiding hose; 9. a box door; 10. A transparent glass window; 11. operating a touch screen; 12. a speed reducer; 121. a load bearing cover; 122. a worm gear; 123. a worm; 124. a drive shaft; 13. a drive member; 14. footing; 15. and (7) a rubber pad.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model provides a stem cell culture device for preparing an anti-aging reagent, which comprises a cell culture box main body 1, wherein a rotatable bearing platform 2 is arranged in the inner cavity of the cell culture box main body 1, a culture dish 3 is arranged at the top end of the bearing platform 2, a plurality of culture dishes 3 are arranged at the side of the top end of the bearing platform 2 at intervals in a ring shape, and the plurality of culture dishes 3 are arranged at the side of the top end of the rotatable bearing platform 2, so that the culture of most stem cells can be simultaneously carried out in the cell culture box main body 1, the working efficiency is improved, a culture solution adding mechanism is arranged at the top end of the cell culture box main body 1, and culture solution is added into the culture dishes 3 to keep the nutrition required by stem cell culture;
the culture solution adding mechanism comprises a culture solution box 4, a discharge hole at the bottom end of the culture solution box 4 is fixedly communicated with a discharge pipe 5, the outer wall of the discharge pipe 5 penetrates through a cell culture box main body 1 and extends to an inner cavity, a flow meter 6 and an electromagnetic valve 7 are fixedly installed at the bottom of the discharge pipe 5, the electromagnetic valve 7 is located below the flow meter 6, the electromagnetic valve 7 is coupled with the flow meter 6, the flow rate is preset through the flow meter 6, when the culture solution passing through the flow meter 6 reaches a preset value, the electromagnetic valve 7 is controlled to be closed by the flow meter 6 to achieve accurate control of the addition amount of the culture solution in a culture dish 3, a discharge port of the electromagnetic valve 7 is fixedly communicated with a guide hose 8, the outer wall of the guide hose 8 is arc-shaped, the guide hose 8 has certain deformability, meanwhile, the side wall is arc-shaped, and the bottom of the guide hose 8 is located at the port of the inner wall at the top of the culture dish 3, the culture dish 3 is in contact with the material guide hose 8 and drives the culture dish to deform when rotating, so that when the bearing platform 2 rotates, the bottom of the material guide hose 8 can be sequentially attached to the top ports of the inner walls of the plurality of culture dishes 3, the culture solution can slide along the inner walls of the culture dishes 3, and the culture solution adding safety is improved;
cell culture case main part 1's front articulates there is chamber door 9, and the top of chamber door 9 is provided with clear glass window 10, sets up clear glass window 10 on the chamber door 9, can be convenient for the operator to observe the condition of inner wall culture dish 3 to the convenient interpolation of fixing a position and culture solution to culture dish 3, and the top of cell culture case main part 1 is provided with operation touch screen 11, and operation touch screen 11 makes things convenient for the switch of each electrical equipment of operator control.
The bottom end of the inner cavity of the cell incubator body 1 is fixedly provided with a speed reducer 12, the axis of the bottom end of the bearing platform 2 is in transmission connection with the output end of the speed reducer 12, the outer wall of the cell incubator body 1 is provided with a driving part 13, the driving part 13 is in transmission connection with the input end of the speed reducer 12, the driving part 13 can be a driving motor or a manual crank, and the driving part is mainly used for driving the bearing platform 2 to rotate according to the use requirement of specific operation;
the reducer 12 comprises a bearing cover 121, a worm wheel 122, a worm 123 and a driving shaft 124, the worm wheel 122 is fixedly sleeved on the outer wall of the driving shaft 124, the outer wall of the worm 123 is meshed with the outer wall of the worm wheel 122, the top end of the driving shaft 124 penetrates through the bearing cover 121 and is fixedly connected with the axis of the bottom end of the bearing table 2, the driving piece 13 drives the worm 123 to rotate, the worm 123 drives the worm wheel 122 to rotate, the worm wheel 122 rotates and simultaneously drives the driving shaft 124 to rotate, so that the driving shaft 124 drives the bearing table 2 to rotate, and therefore the purpose of speed reduction is achieved, and meanwhile, a reversing driving effect is achieved;
the equal fixedly connected with footing 14 in the edge of 1 bottom of cell culture case main part, the equal fixedly connected with rubber pad 15 in the bottom of four footing 14, footing 14 and the 15 cooperation of rubber pad are used, can improve its stability and silence effect when supporting cell culture case main part 1.
The working principle of the utility model is as follows: when culture solution needs to be added into a plurality of culture dishes 3 on the bearing platform 2, an operator only needs to operate the driving piece 13 to drive the bearing platform 2 to rotate through the speed reducer 12, so that the plurality of culture dishes 3 on the side of the top end of the bearing platform 2 synchronously rotate, when one culture dish 3 moves to the position under the discharging pipe 5, the bottom port of the material guiding hose 8 is attached to the port of the inner wall of the top of the culture dish 3, then the operator sets the preset flow of the flow meter 6 through operating the touch screen 11, when the culture solution in the culture solution box 4 flows along the discharging pipe 5, the flow meter 6 monitors the flow of the passing culture solution, when the culture solution flowing through the flow meter 6 reaches the preset amount, the flow meter 6 controls the electromagnetic valve 7 to be closed, so that the quantitative culture solution adding in the culture dishes 3 is realized, then the operator drives the bearing platform 2 to rotate through the driving piece 13, so that the next culture dish 3 rotates to the position under the discharging pipe 5, the addition of the culture solution to the next culture dish 3 is started, and the operation of adding the culture solution to the plurality of culture dishes 3 is completed.
In the description of the present invention, unless otherwise expressly specified or limited, the terms "disposed," "mounted," "connected," and "secured" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integral to; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The standard parts used by the utility model can be purchased from the market, and the special-shaped parts can be customized according to the description and the description of the attached drawings.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A stem cell culture device for preparing an anti-aging reagent comprises a cell culture box main body (1) and is characterized in that a rotatable bearing platform (2) is arranged in an inner cavity of the cell culture box main body (1), a culture dish (3) is arranged at the top end of the bearing platform (2), and a culture solution adding mechanism is arranged at the top end of the cell culture box main body (1);
culture solution adds mechanism includes culture solution box (4), the fixed intercommunication of discharge gate of culture solution box (4) bottom has discharging pipe (5), just the outer wall of discharging pipe (5) runs through cell culture box main part (1) and extends to the inner chamber, the bottom fixed mounting of discharging pipe (5) has flowmeter (6) and solenoid valve (7), just solenoid valve (7) are located flowmeter (6) below position.
2. The stem cell culture device for preparing the anti-aging reagent according to claim 1, wherein a material guiding hose (8) is fixedly communicated with a discharge port of the electromagnetic valve (7), and the outer wall of the material guiding hose (8) is arc-shaped.
3. The stem cell culture device for preparing anti-aging agent according to claim 1, characterized in that the front of the cell incubator main body (1) is hinged with a door (9), the top of the door (9) is provided with a transparent glass window (10), and the top end of the cell incubator main body (1) is provided with an operation touch screen (11).
4. The stem cell culture device for preparing the anti-aging reagent according to claim 1, wherein a speed reducer (12) is fixedly arranged at the bottom end of the inner cavity of the cell culture box main body (1), the axis of the bottom end of the bearing platform (2) is in transmission connection with the output end of the speed reducer (12), a driving piece (13) is arranged on the outer wall of the cell culture box main body (1), and the driving piece (13) is in transmission connection with the input end of the speed reducer (12).
5. The stem cell culture device for preparing the anti-aging reagent according to claim 4, wherein the speed reducer (12) comprises a bearing cover (121), a worm wheel (122), a worm (123) and a driving shaft (124), the worm wheel (122) is fixedly sleeved on the outer wall of the driving shaft (124), the outer wall of the worm (123) is meshed with the outer wall of the worm wheel (122), and the top end of the driving shaft (124) penetrates through the bearing cover (121) and is fixedly connected with the bottom end of the bearing platform (2).
6. The stem cell culture device for preparing anti-aging agent according to claim 1, wherein the culture dish (3) is provided in plurality, and the plurality of culture dishes (3) are arranged at the side of the top end of the bearing platform (2) at annular intervals.
7. The stem cell culture device for preparing anti-aging agent according to claim 1, wherein the bottom end of the cell culture box body (1) is fixedly connected with feet (14) at the corners, and rubber pads (15) are fixedly connected with the bottom ends of the four feet (14).
CN202122066569.6U 2021-08-30 2021-08-30 Stem cell culture device is used in preparation of anti-aging reagent Expired - Fee Related CN215517475U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122066569.6U CN215517475U (en) 2021-08-30 2021-08-30 Stem cell culture device is used in preparation of anti-aging reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122066569.6U CN215517475U (en) 2021-08-30 2021-08-30 Stem cell culture device is used in preparation of anti-aging reagent

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CN215517475U true CN215517475U (en) 2022-01-14

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114621872A (en) * 2022-02-24 2022-06-14 河南茵特赛尔生物技术有限公司 Automatic liquid supplementing device for immune cell culture solution
CN115125138A (en) * 2022-07-21 2022-09-30 西安交通大学医学院第一附属医院 Three-dimensional culture apparatus of tumour stem cell

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114621872A (en) * 2022-02-24 2022-06-14 河南茵特赛尔生物技术有限公司 Automatic liquid supplementing device for immune cell culture solution
CN114621872B (en) * 2022-02-24 2022-09-16 河南茵特赛尔生物技术有限公司 Automatic liquid supplementing device for immune cell culture solution
CN115125138A (en) * 2022-07-21 2022-09-30 西安交通大学医学院第一附属医院 Three-dimensional culture apparatus of tumour stem cell

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