CN113265329A

CN113265329A - Biological cell culture box and use method thereof

Info

Publication number: CN113265329A
Application number: CN202110384190.1A
Authority: CN
Inventors: 丁海雷; 孙三勇; 黄冠; 梅娟
Original assignee: Jiangsu Beike Biotechnology Co ltd
Current assignee: Zhongrun Huayu Biopharmaceutical Xiamen Co ltd
Priority date: 2021-04-09
Filing date: 2021-04-09
Publication date: 2021-08-17
Anticipated expiration: 2041-04-09
Also published as: CN113265329B

Abstract

The invention discloses a biological cell incubator and a use method thereof, wherein the incubator comprises: the box body is fixedly connected with a bottom plate below the box body, and the top of the box body is fixedly connected with a top plate; the translation device is positioned inside the box body and comprises sliding grooves, sliding plates are arranged inside the sliding grooves, a placing groove is formed in each sliding plate, a culture dish is arranged inside each placing groove, and through holes are formed in the sliding plates positioned inside the placing grooves; the taking and placing device is positioned on one side of the translation device in the box body and comprises a supporting rod, the two sides of the box body are provided with slideways at positions corresponding to the two ends of the supporting rod, and the supporting rod is in limited sliding connection with the slideways; the invention can effectively protect biological cells and prevent the cells from being polluted when the cells are taken and placed.

Description

Biological cell culture box and use method thereof

Technical Field

The invention belongs to the technical field of cell culture boxes, and particularly relates to a biological cell culture box and a using method thereof.

Background

The cell culture technology is an important and common technology in cell biology research methods, a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of the cells and the like of the cells can be researched, a cell culture box is often required to be used in the cell culture process, but the common cell culture box has a plurality of problems in practical use:

1. for example, more cultured cells are generally arranged into a plurality of layers, each layer is separated by a partition plate, and a plurality of culture dishes are placed on each layer, but the trouble is brought to the taking and placing of the rear culture dishes by workers, and the cells are easily polluted during operation;

2. due to the different heights of the culture dishes, the distance between the partition plates is difficult to adjust according to the needs.

Disclosure of Invention

Technical problem to be solved

The invention provides a biological cell incubator and a using method thereof, and aims to solve the problems in the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

a biological cell incubator comprising:

the box body is fixedly connected with a bottom plate below the box body, and the top of the box body is fixedly connected with a top plate;

the translation device is positioned inside the box body and comprises sliding grooves, sliding plates are arranged inside a plurality of groups of sliding grooves, a placing groove is formed in each sliding plate, a culture dish is arranged inside each placing groove, and through holes are formed in the sliding plates positioned inside the placing grooves;

the taking and placing device is positioned on one side of the translation device in the box body and comprises a supporting rod, the positions of the two sides of the box body, which correspond to the two ends of the supporting rod, are respectively provided with a slideway, and the supporting rod is in limited sliding connection with the slideways; the invention can effectively protect biological cells and prevent the cells from being polluted when the cells are taken and placed.

As a preferable scheme of the invention, the top plate is distributed in a step shape, an outlet is formed in the middle of the top plate, and a closing device is arranged on the outlet.

As a preferable scheme of the invention, the closing device comprises a closing plate, the size of the closing plate corresponds to the size of the outlet, one end of the closing plate is in limit movable connection with the top plate through a hinge, a telescopic cylinder is arranged at the position of the top plate corresponding to the closing plate, and the output end of the telescopic cylinder is in limit movable connection with the closing plate.

As a preferable scheme of the invention, a plurality of groups of sliding chutes which are uniformly distributed are arranged on the inner walls of the two sides of the box body, the lengths of the sliding chutes are different, the lengths of the sliding chutes are sequenced from high to low according to a certain proportion, and the sliding chutes and the sliding plates are arranged in a limiting sliding manner.

As a preferable scheme of the invention, gears are arranged inside the sliding grooves and above the sliding plates, gear grooves are formed in the positions of the sliding plates corresponding to the gears, and the gears are in meshed connection with the gear grooves.

As a preferred scheme of the invention, the middle parts of the gears are provided with central rods, the central rods extend to the outside through the box body, the box body is provided with translation motors at positions corresponding to the central rods, the output ends of the translation motors are in transmission connection with the central rods through couplers, and the translation motors are fixedly connected with the box body through bolts.

As a preferable scheme of the invention, the middle part of the supporting rod is provided with a screw hole, the middle part of the supporting rod is connected with a screw rod through the screw hole and a thread, the top of the screw rod penetrates through the top plate and is in limit movable connection with the top plate through a rotating shaft, the bottom of the screw rod is provided with a limit block, and the limit block is in limit movable connection with the screw rod through the rotating shaft.

As a preferable scheme of the invention, a lifting motor is arranged at a position of the top plate corresponding to the screw rod, an output end of the lifting motor is in transmission connection with the screw rod through a coupler, the lifting motor is fixedly connected with the top plate through a bolt, one side of the support rod is provided with a group of support rods corresponding to the through holes, and the size of each support rod is slightly smaller than that of the through hole.

A biological cell incubator and a use method thereof specifically comprise the following steps:

s1: the output end of the telescopic cylinder pushes the closing plate to open the outlet;

s2: the output end of the translation motor drives the central rod to rotate, so that the gear can be meshed with the gear groove, the gear groove drives the sliding plate to move in the sliding groove, and the sliding plate drives the culture dish to move in position;

s3: the sliding plate is moved to the tail end of the sliding groove, and the plurality of culture dishes cannot be influenced by the different lengths of the sliding grooves;

s4: when putting the culture dish, the output through elevator motor drives the screw rod and rotates, makes the bracing piece translation about can going on the screw rod, through the branch that sets up, can pass through the through-hole department and support the culture dish, drives the culture dish and reciprocates.

The invention has the technical effects and advantages that:

(1) when the culture dish is used, the output end of the telescopic cylinder pushes the closing plate to rotate along the hinge, the opening and closing arrangement of the outlet is controlled, the output end of the translation motor drives the central rod to rotate, the central rod drives the gear to rotate, the gear can be meshed and connected with the gear groove, when the position of the gear is kept unchanged, the gear groove can move along the rotation direction of the gear, the gear groove drives the sliding plate to move in the sliding groove, and the sliding plate drives the culture dish to move;

(2) in the invention, the sliding plates can be moved to the tail ends of the sliding grooves, and the sliding plates can not be vertically overlapped through the different lengths of the sliding grooves, so that a plurality of culture dishes can not be mutually influenced, and ventilation is facilitated;

(3) according to the invention, when the culture dish is taken and placed, the screw rod is driven to rotate through the output end of the lifting motor, the screw rod is in threaded connection with the screw rod through the screw hole, the supporting rod can move on the screw rod when the position of the screw rod is unchanged, and meanwhile, the supporting rod is limited through the limiting sliding connection between the supporting rod and the slide way, so that the supporting rod can translate up and down on the screw rod, the supporting rod drives the supporting rod to move, the culture dish can be supported through the through hole through the arranged supporting rod, the culture dish is driven to move up and down at the same time, and the culture dish can be moved out from the outlet, so that the cells are prevented from being polluted.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a closure device according to the present invention;

FIG. 3 is a schematic view of the sectional three-dimensional structure inside the case of the present invention;

FIG. 4 is a schematic view of the internal cross-sectional side view of the housing of the present invention;

FIG. 5 is an enlarged view of the structure at A in FIG. 3 according to the present invention;

fig. 6 is a schematic view of the sectional structure of the inside of the chute of the present invention.

The reference numbers in the figures illustrate:

1. a base plate; 2. a box body; 3. a top plate; 31. an outlet;

4. a closure device; 41. a closing plate; 42. a hinge; 43. a telescopic cylinder;

5. a translation device; 51. a chute; 52. a slide plate; 53. a placement groove; 54. a culture dish; 55. a port; 56. a gear; 57. a gear groove; 58. a center pole; 59. a translation motor;

6. a pick-and-place device; 61. a support bar; 62. a slideway; 63. a screw hole; 64. a screw; 65. a limiting block; 66. a strut; 67. a lifting motor.

Detailed Description

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In an embodiment, referring to fig. 1-6, a biological cell incubator comprises:

the box body 2 is fixedly connected with a bottom plate 1 below the box body 2, and the top of the box body 2 is fixedly connected with a top plate 3;

the translation device 5 is positioned inside the box body 2, the translation device 5 comprises sliding chutes 51, sliding plates 52 are arranged inside the plurality of groups of sliding chutes 51, placing grooves 53 are formed in the sliding plates 52, culture dishes 54 are arranged inside the placing grooves 53, and through holes 55 are formed in the sliding plates 52 positioned inside the placing grooves 53;

the taking and placing device 6 is located on one side of the translation device 5 inside the box body 2, the taking and placing device 6 comprises a supporting rod 61, the positions, located at the two ends of the corresponding supporting rod 61, of the two sides of the box body 2 are both provided with a slide way 62, and the supporting rod 61 is in limiting sliding connection with the slide way 62.

In the specific embodiment of the invention, the box body 2 is used for limiting and supporting the whole body, the box body 2 is made of transparent materials, so that the interior of the box body 2 can be observed finely, and the box body 2 can be in a closed state through the bottom plate 1 and the top plate 3; the sliding plate 52 can be conveniently moved to a specific position through the arranged translation device 5, the culture dish 54 is limited and supported through the sliding plate 52, the culture dish 54 is used for culturing biological cells, so that the biological cells can be kept stable, and the culture dish 54 can be conveniently taken and placed through the arranged through hole 55; the culture dish 54 can be automatically taken and placed by the taking and placing device 6 without manual taking, so that the cells are prevented from being polluted by bacteria.

Specifically, referring to fig. 1, the top plate 3 is distributed in a step shape, an outlet 31 is formed in the middle of the top plate 3, and a closing device 4 is disposed on the outlet 31.

In a further embodiment of the invention: through distributing roof 3 ladder, can make bracing piece 61 pass through export 31 with culture dish 54, see off inside the box 2, make personnel not contact with box 2 is inside, prevent to cause the pollution to the cell, when not getting and putting the cell, can completely cut off box 2 and external through closing device 4.

Specifically, referring to fig. 2, the closing device 4 includes a closing plate 41, the size of the closing plate 41 is set corresponding to the size of the outlet 31, one end of the closing plate 41 is movably connected to the top plate 3 through a hinge 42 in a limiting manner, a telescopic cylinder 43 is disposed at a position of the top plate 3 corresponding to the closing plate 41, and an output end of the telescopic cylinder 43 is movably connected to the closing plate 41 in a limiting manner.

In a further embodiment of the invention: the closing plate 41 is used for shielding the outlet 31, the hinge 42 is used for limiting the position of the closing plate 41, the telescopic cylinder 43 is started, the telescopic cylinder 43 can be selected to be different types according to actual conditions, for example, the type is selected to be SC50, the closing plate 41 is pushed by the output end of the telescopic cylinder 43, the closing plate 41 rotates along the hinge 42, and the outlet 31 is opened and closed.

Specifically, referring to fig. 3, a plurality of sets of sliding grooves 51 are uniformly distributed on the inner walls of the two sides of the box body 2, the lengths of the plurality of sets of sliding grooves 51 are different, the lengths of the sliding grooves 51 are sorted from high to low according to a certain proportion, and the sliding grooves 51 and the sliding plates 52 are arranged in a limiting sliding manner.

In a further embodiment of the invention: the sliding grooves 51 are arranged in different lengths, so that the sliding plates 52 are not vertically overlapped.

Specifically, referring to fig. 6, gears 56 are disposed inside the sliding grooves 51 above the sliding plates 52, gear grooves 57 are formed in the positions of the sliding plates 52 corresponding to the gears 56, and the gears 56 are engaged with the gear grooves 57.

In a further embodiment of the invention: by engaging and connecting the gear 56 and the gear groove 57, when the position of the gear 56 is kept unchanged, the gear groove 57 can move along the rotation direction of the gear 56, and the gear groove 57 can drive the sliding plate 52 to move in the sliding groove 51.

Specifically, please refer to fig. 6, the middle portion of the gear 56 is provided with a central rod 58, the central rod 58 extends to the outside through the box body 2, the box body 2 is provided with a translation motor 59 at a position corresponding to the central rod 58, an output end of the translation motor 59 is in transmission connection with the central rod 58 through a coupling, and the translation motor 59 is fixedly connected with the box body 2 through a bolt.

In a further embodiment of the invention: position fixing can be carried out to gear 56 through well pole 58 that sets up, starts translation motor 59, and translation motor 59 can select different models according to actual conditions, for example selects the model to be 3GN150K, and output through translation motor 59 drives well pole 58 and rotates, drives gear 56 through well pole 58 and rotates, and gear 56 rotates and can be connected with the meshing between the gear groove 57.

Specifically, referring to fig. 4, a screw hole 63 is formed in the middle of the support rod 61, a screw rod 64 is connected to the middle of the support rod 61 through the screw hole 63, the top of the screw rod 64 penetrates through the top plate 3 and is movably connected to the top plate 3 through a rotating shaft, a limit block 65 is arranged at the bottom of the screw rod 64, and the limit block 65 is movably connected to the screw rod 64 through a rotating shaft.

In a further embodiment of the invention: through threaded connection between screw 63 and the screw rod 64, when can making the screw rod 64 position unchangeable, bracing piece 61 can carry out the position on screw rod 64 and remove, spacing sliding connection comes to carry out spacing to bracing piece 61 through between bracing piece 61 and the slide 62 simultaneously, makes bracing piece 61 can carry out translation from top to bottom on screw rod 64, can carry out spacing setting to screw rod 64 through roof 3 and stopper 65.

Specifically, referring to fig. 4, a lifting motor 67 is disposed at a position of the top plate 3 corresponding to the screw 64, an output end of the lifting motor 67 is in transmission connection with the screw 64 through a coupler, the lifting motor 67 is fixedly connected with the top plate 3 through a bolt, a group of supporting rods 66 corresponding to the through holes 55 is disposed at one side of the supporting rod 61, and the size of the supporting rods 66 is slightly smaller than that of the through holes 55.

In a further embodiment of the invention: drive screw rod 64 through elevator motor 67, start elevator motor 67, elevator motor 67 can select different models according to actual conditions, for example, select the model to be 3IK15RGN-C, output through elevator motor 67 drives screw rod 64 and rotates, drive bracing piece 61 through screw rod 64 and carry out translation from top to bottom on screw rod 64, make bracing piece 61 drive branch 66 and remove, through the branch 66 that sets up, can pass opening 55 department and support culture dish 54, it reciprocates to drive culture dish 54 simultaneously.

s1: the output end of the telescopic cylinder 43 pushes the closing plate 41, so that the closing plate 41 rotates along the hinge 42 to open the outlet 31;

s2: the output end of the translation motor 59 drives the central rod 58 to rotate, the central rod 58 drives the gear 56 to rotate, so that the gear 56 can be meshed with the gear groove 57, when the position of the gear 56 is kept unchanged, the gear groove 57 drives the sliding plate 52 to move in the sliding groove 51, and the sliding plate 52 drives the culture dish 54 to move;

s3: the slide plate 52 is moved to the tail end of the slide groove 51, and the lengths of the slide grooves 51 are different, so that the plurality of culture dishes 54 cannot be influenced;

s4: when putting culture dish 54, the output through elevator motor 67 drives screw rod 64 and rotates, through threaded connection between screw 63 and the screw rod 64, makes bracing piece 61 carry out translation from top to bottom on screw rod 64, makes bracing piece 61 drive branch 66 and removes, through the branch 66 that sets up, can pass through opening 55 department and support culture dish 54, drives culture dish 54 simultaneously and reciprocates.

The working principle of the embodiment is as follows: when the biological cell incubator is used, the telescopic cylinder 43 is started, the closing plate 41 is pushed by the output end of the telescopic cylinder 43, the closing plate 41 rotates along the hinge 42, and the outlet 31 is controlled to be opened and closed;

starting the translation motor 59, driving the central rod 58 to rotate through the output end of the translation motor 59, driving the gear 56 to rotate through the central rod 58, enabling the gear 56 to be in meshed connection with the gear groove 57, and enabling the gear groove 57 to move along the rotation direction of the gear 56 when the position of the gear 56 is kept unchanged, enabling the gear groove 57 to drive the sliding plate 52 to move inside the sliding groove 51, and driving the culture dish 54 to move through the sliding plate 52;

the sliding plates 52 are moved to the tail ends of the sliding grooves 51, and the sliding plates 52 can not be vertically overlapped through the different lengths of the sliding grooves 51, so that the air circulation is improved;

when the culture dish 54 is taken and placed, the lifting motor 67 is started, the screw rod 64 is driven to rotate through the output end of the lifting motor 67, the screw hole 63 is in threaded connection with the screw rod 64, the support rod 61 can move on the screw rod 64 when the position of the screw rod 64 is unchanged, meanwhile, the support rod 61 is limited through the limiting sliding connection between the support rod 61 and the slide way 62, the support rod 61 can move up and down on the screw rod 64, the support rod 61 drives the support rod 66 to move, the culture dish 54 can pass through the through hole 55 to be supported through the arranged support rod 66, and the culture dish 54 is driven to move up and down and can be moved out from the outlet 31; the invention can effectively protect biological cells and prevent the cells from being polluted when the cells are taken and placed.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A biological cell incubator, comprising:

the box body (2), a bottom plate (1) is fixedly connected below the box body (2), and a top plate (3) is fixedly connected to the top of the box body (2);

the horizontal moving device (5) is positioned inside the box body (2), the horizontal moving device (5) comprises sliding chutes (51), sliding plates (52) are arranged inside a plurality of groups of sliding chutes (51), placing grooves (53) are formed in the sliding plates (52), culture dishes (54) are arranged inside the placing grooves (53), and through holes (55) are formed in the sliding plates (52) positioned inside the placing grooves (53);

get and put device (6), be located one side of the inside translation device (5) of box (2), get and put device (6) and include bracing piece (61), slide (62) have all been opened in the position that the both sides of box (2) are located corresponding bracing piece (61) both ends, spacing sliding connection between bracing piece (61) and slide (62).

2. A biological cell incubator according to claim 1, wherein: the roof (3) are distributed in a step mode, an outlet (31) is formed in the middle of the roof (3), and a closing device (4) is arranged on the outlet (31).

3. A biological cell incubator according to claim 2, wherein: closing device (4) are including closing plate (41), the size of closing plate (41) sets up with the size of export (31) is corresponding, through the spacing swing joint of hinge (42) between one end of closing plate (41) and roof (3), the position that roof (3) are located corresponding closing plate (41) is provided with telescopic cylinder (43), spacing swing joint between the output of telescopic cylinder (43) and closing plate (41).

4. A biological cell incubator according to claim 1, wherein: the box (2) all open on the inner wall of both sides has multiunit evenly distributed's spout (51), the multiunit the length inequality of spout (51), the length of spout (51) is in proper proportion from high to low to sort, spacing slip setting between spout (51) and slide (52).

5. A biological cell incubator according to claim 4, wherein: the gear (56) is arranged above the sliding plate (52) in the sliding groove (51), the gear grooves (57) are formed in the positions, corresponding to the gear (56), of the sliding plate (52), and the gear (56) is meshed with the gear grooves (57).

6. A biological cell incubator according to claim 5, characterised in that: the middle part of gear (56) all is provided with well core rod (58), well core rod (58) all runs through box (2) and extends to the outside, the position that box (2) are located to correspond well core rod (58) all is provided with translation motor (59), be connected through the shaft coupling transmission between the output of translation motor (59) and well core rod (58), all through bolt fixed connection between translation motor (59) and box (2).

7. A biological cell incubator according to claim 1, wherein: screw (63) are opened at the middle part of bracing piece (61), there is screw rod (64) at the middle part of bracing piece (61) through screw (63) threaded connection, the top of screw rod (64) run through roof (3) and with roof (3) between through the spacing swing joint of pivot, the bottom of screw rod (64) is provided with stopper (65), through the spacing swing joint of pivot between stopper (65) and screw rod (64).

8. A biological cell incubator according to claim 7, wherein: roof (3) are located the position that corresponds screw rod (64) and are provided with elevator motor (67), be connected through the shaft coupling transmission between the output of elevator motor (67) and screw rod (64), pass through bolt fixed connection between elevator motor (67) and roof (3), one side of bracing piece (61) is provided with a set of branch (66) corresponding with opening (55), the size of branch (66) is compared a little littlely with the size of opening (55).

9. A biological cell incubator and method of use thereof as claimed in any one of claims 1 to 8, including the steps of:

s1: the closing plate (41) is pushed by the output end of the telescopic cylinder (43), and the outlet (31) is opened;

s2: the output end of the translation motor (59) drives the central rod (58) to rotate, so that the gear (56) can be meshed with the gear groove (57), the gear groove (57) drives the sliding plate (52) to move in the sliding groove (51), and the sliding plate (52) drives the culture dish (54) to move in position;

s3: the sliding plate (52) is moved to the tail end of the sliding chute (51), and the lengths of the sliding chutes (51) are different, so that the plurality of culture dishes (54) cannot be influenced mutually;

s4: when putting culture dish (54) in and putting, output through elevator motor (67) drives screw rod (64) and rotates, makes bracing piece (61) can carry out translation from top to bottom on screw rod (64), through branch (66) that sets up, can pass through opening (55) and locate to support culture dish (54), drives culture dish (54) and reciprocates.