CN117586871B - Temperature control equipment for cell culture - Google Patents
Temperature control equipment for cell culture Download PDFInfo
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- CN117586871B CN117586871B CN202410081393.7A CN202410081393A CN117586871B CN 117586871 B CN117586871 B CN 117586871B CN 202410081393 A CN202410081393 A CN 202410081393A CN 117586871 B CN117586871 B CN 117586871B
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- 238000004113 cell culture Methods 0.000 title claims abstract description 28
- 238000007789 sealing Methods 0.000 claims description 7
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 6
- 239000013013 elastic material Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000012774 insulation material Substances 0.000 claims description 2
- 238000000151 deposition Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 9
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004308 accommodation Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses temperature control equipment for cell culture, and belongs to the technical field of cell culture. Mainly including cultivateing the cabinet, one side of cultivateing the cabinet is provided with the cabinet mouth, cabinet mouth inboard distribution has a plurality of holding tanks, one side rotation of cabinet mouth is provided with the cabinet door, the holding tank is inside all to be provided with one side open-ended smooth cabinet, the opening part of holding tank all is provided with the apron, the apron is suitable for under outside drive to cover or open the opening of holding tank, the inboard bottom of smooth cabinet is provided with a plurality of storage tanks, one side fixed mounting of cultivateing the cabinet has temperature control device, when depositing the cell, the control cover is all closed, open the cabinet door again, select to open the apron of required position, make the apron of other positions can keep smooth cabinet inside airtight, thereby avoid the storage environment of the cell that keeps before in the other smooth cabinets to change, and influence the cell in cultivateing. The temperature control equipment for cell culture provided by the application has the effect of reducing the change of other cell culture environments when cells are accessed.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to temperature control equipment for cell culture.
Background
Cell culture refers to a technique of culturing cells taken out of an organism in vitro to simulate the physiological environment in vivo, and allowing them to survive and grow. In cell culture, it is often necessary to control the temperature of the cell culture environment.
However, when the cabinet door is opened, the interior of the culture cabinet is communicated with the outside, so that the environment in the culture cabinet is changed, and the original stem cell culture in the culture cabinet is influenced.
It is therefore necessary to provide a temperature control device for cell culture to solve the above-mentioned problems.
Disclosure of Invention
Based on the above-mentioned problems existing in the prior art, an object of an embodiment of the present application is to: a temperature control device for cell culture is provided, which can reduce the change of other cell culture environment when accessing cells.
The technical scheme adopted for solving the technical problems is as follows: the utility model provides a cell culture is with control by temperature change equipment, includes cultivates the cabinet, one side of cultivateing the cabinet is provided with the cabinet mouth, cabinet mouth inboard distribution has a plurality of holding tanks, one side rotation of cabinet mouth is provided with the cabinet door, the inside smooth cabinet that all is provided with one side open-ended of holding tank, the opening part of smooth cabinet all is provided with the apron towards the cabinet mouth, the opening part of holding tank, the apron is suitable for under external drive to cover or open the opening of holding tank, the inboard bottom of smooth cabinet is provided with a plurality of storage tanks, one side fixed mounting of cultivateing the cabinet has temperature control device, temperature control device and cultivate the intra-cabinet intercommunication.
Further, the bottom of holding tank is provided with the spout, the downside of smooth cabinet is fixed with the slider, be fixed with a plurality of slide bars between the both ends of spout, the slide bar all runs through the slider, slide bar and slider sliding connection, rotate between the both ends of spout and be connected with the actuating lever, the outer wall fixed mounting that cabinet mouth one side was kept away from to cultivate the cabinet has a plurality of drivers that correspond with the actuating lever position, the output shaft and the actuating lever of driver are fixed, the actuating lever runs through the slider, actuating lever and slider threaded connection.
Further, the both sides of cabinet mouth all are provided with the drive groove, be connected with a plurality of rotation axles between the drive groove, the downside of holding tank all is provided with the exposure groove, the exposure groove all is run through by the rotation axle, the opening part of holding tank all is provided with the apron, the downside of apron is fixed with the axle sleeve, the axle sleeve all rotates the cover and locates on the rotation axle in the holding tank.
Further, the rotation shaft rotates and sets up between the drive groove, be fixed with the ejector pad that corresponds with the axle sleeve position on the rotation shaft, the one end of axle sleeve is provided with the ejector pad hole, the ejector pad is located the ejector pad downthehole, one side of ejector pad hole inner wall is fixed with the dog.
Further, gears are fixed at two ends of the rotating shaft, the gears are located in the driving grooves, a sliding rail is fixed on one side of each driving groove, a rack is arranged in each sliding rail in a sliding mode, and the gears are meshed with the racks.
Further, the upside of cultivateing the cabinet is provided with the lift groove, be provided with the lifter plate in the lift groove, the upper end of rack is all fixed with the lifter plate, the upside of lift groove all is fixed with the telescopic link, the output pole and the lifter plate of telescopic link are fixed.
Further, an observation plate is fixed in the middle of the cabinet door and made of transparent materials, an electric buckle is connected between one side of the cabinet door and the culture cabinet, a sealing ring is fixed on one side of the cover plate, which is close to the accommodating groove, and made of elastic materials, and the cover plate, the culture cabinet and the cabinet door are made of heat insulation materials;
the upper side of smooth cabinet opening one end has been seted up the apron groove, be provided with the baffle in the apron groove, the one end that the cabinet mouth was kept away from to the baffle rotates with the apron inslot wall to be connected, the bottom of apron groove both sides all is fixed with the supporting shoe, the supporting shoe is located the downside of baffle.
Further, one side of cultivateing the cabinet is provided with control panel, the upside of apron all is fixed with first magnetite, the upside of holding tank is fixed with the second magnetite that corresponds with first magnetite position, all be fixed with first temperature detector in the smooth cabinet, cultivate the cabinet internal fixation and be fixed with second temperature detector.
The beneficial effects of the invention are as follows:
In the cell culture process, the cover plate is kept in an open state, so that the temperature control device can conveniently control the temperature of the inside of the culture cabinet and the inside of the sliding cabinet simultaneously;
when the cells are stored and taken out, the cover plates are controlled to be closed completely, then the cabinet door is opened, and the cover plates at the required positions are selected to be opened, so that the cover plates at other positions can keep the interior of the sliding cabinet airtight, and the change of the storage environment of the cells stored in the sliding cabinet before can be avoided, and the cells in culture can be prevented from being influenced;
After the cell storage and taking is finished, the cabinet door is closed, the temperature control device adjusts the inside of the culture cabinet to the culture temperature, and the rest cover plates are opened, so that the culture temperature of all cells can be controlled conveniently.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. In the drawings:
FIG. 1 is a schematic diagram showing an overall temperature control apparatus for cell culture according to the present application;
FIG. 2 is a schematic diagram showing an opened state of a cabinet door of the temperature control apparatus for cell culture of FIG. 1;
FIG. 3 is an enlarged schematic view of FIG. 2 at A;
FIG. 4 is a schematic view showing the internal structure of the receiving groove in FIG. 2;
FIG. 5 is an enlarged schematic view at B in FIG. 4;
FIG. 6 is an overall schematic of the rack of FIG. 2;
FIG. 7 is an enlarged schematic view of FIG. 6 at C;
FIG. 8 is an enlarged schematic view of FIG. 6 at D;
FIG. 9 is an overall schematic view of the rotation shaft of FIG. 4;
In the figure:
1. A culture cabinet; 11. a cabinet opening; 12. a receiving groove; 121. a chute; 122. a slide bar; 123. a driving rod; 124. a driver; 125. a second magnet; 13. a cabinet door; 131. an observation plate; 132. an electric buckle; 14. a driving groove; 141. a rotation shaft; 142. a pushing block; 143. a gear; 144. a slide rail; 145. a rack; 15. exposing the groove; 16. a lifting groove; 161. a lifting plate; 162. a telescopic rod; 17. a second temperature detector;
2. A control panel;
3. A sliding cabinet; 31. a slide block; 32. a cover plate groove; 33. a baffle; 34. a support block; 35. a storage tank; 36. a first temperature detector;
4. a cover plate; 41. a shaft sleeve; 42. a first magnet; 43. a push block hole; 44. a stop block; 45. a seal ring;
5. and a temperature control device.
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will be described in detail below with reference to the drawings in connection with embodiments.
It is noted that all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless otherwise indicated.
In the present invention, unless otherwise indicated, the terms "upper" and "lower" are used generally with respect to the directions shown in the drawings, or with respect to the vertical, vertical or gravitational directions; also, for ease of understanding and description, "left, right" is generally directed to the left, right as shown in the drawings; "inner and outer" refer to inner and outer relative to the outline of the components themselves, but the above-described orientation terms are not intended to limit the present invention.
As shown in fig. 1-9, the application provides a temperature control device for cell culture, which comprises a culture cabinet 1, wherein a cabinet opening 11 is arranged on one side of the culture cabinet 1, a plurality of accommodating grooves 12 are distributed on the inner side of the cabinet opening 11, and a cabinet door 13 is rotatably arranged on one side of the cabinet opening 11.
An observation plate 131 is fixed in the middle of the cabinet door 13, and the observation plate 131 is made of transparent materials. So that when the cabinet door 13 covers the cabinet opening 11, a worker can observe the inside of the culture cabinet 1 through the observation plate 131.
An electric buckle 132 is connected between one side of the cabinet door 13 and the culture cabinet 1.
One side of the culture cabinet 1 is provided with a control panel 2.
The holding tank 12 is inside all to be provided with one side open-ended smooth cabinet 3, and the opening of smooth cabinet 3 is towards cabinet mouth 11.
The bottom of the accommodating groove 12 is provided with a sliding groove 121, and the accommodating groove 12, the sliding groove 121 and the length direction of the sliding cabinet 3 are all parallel.
The sliding block 31 is fixed on the lower side of the sliding cabinet 3, a plurality of sliding rods 122 are fixed between two ends of the sliding groove 121, the sliding rods 122 penetrate through the sliding block 31, and the sliding rods 122 are in sliding connection with the sliding block 31.
A driving rod 123 is rotatably connected between two ends of the sliding groove 121, a plurality of drivers 124 corresponding to the positions of the driving rods 123 are fixedly arranged on the outer wall of one side, far away from the cabinet opening 11, of the culture cabinet 1, in the embodiment, the drivers 124 are motors, an output shaft of each driver 124 is fixed with each driving rod 123, each driving rod 123 penetrates through the sliding block 31, and each driving rod 123 is in threaded connection with the sliding block 31.
The axes of the slide bar 122 and the driving bar 123 are parallel to the longitudinal direction of the accommodating groove 12.
The upper side of the opening end of the sliding cabinet 3 is provided with a cover plate groove 32, a baffle plate 33 is arranged in the cover plate groove 32, and one end of the baffle plate 33 far away from the cabinet opening 11 is rotationally connected with the inner wall of the cover plate groove 32.
The bottoms of the two sides of the cover plate groove 32 are respectively fixed with a supporting block 34, and the supporting blocks 34 are positioned on the lower sides of the baffle plates 33.
The bottom of the inner side of the sliding cabinet 3 is provided with a plurality of storage tanks 35, and the storage tanks 35 are the storage tanks of the stem cell culture container.
The both sides of cabinet mouth 11 all are provided with drive slot 14, rotate between the drive slot 14 and be connected with a plurality of rotation axles 141. The lower sides of the accommodating grooves 12 are provided with exposing grooves 15, and the exposing grooves 15 are penetrated by the rotation shafts 141. The opening part of the accommodating groove 12 is provided with a cover plate 4, the lower side of the cover plate 4 is fixed with a shaft sleeve 41, and the shaft sleeve 41 is rotationally sleeved on a rotating shaft 141 in the accommodating groove 12.
The first magnets 42 are fixed to the upper side of the cover plate 4, and the second magnets 125 corresponding to the first magnets 42 are fixed to the upper side of the accommodation groove 12.
A push block 142 corresponding to the position of the shaft sleeve 41 is fixed on the rotating shaft 141, a push block hole 43 is formed in one end of the shaft sleeve 41, the push block 142 is positioned in the push block hole 43, and a stop block 44 is fixed on one side of the inner wall of the push block hole 43.
A sealing ring 45 is fixed to the side of the cover plate 4 close to the accommodation groove 12, the sealing ring 45 is made of an elastic material, and in this embodiment, the sealing ring 45 is made of rubber. The cover plate 4, the culture cabinet 1 and the cabinet door 13 are all made of heat-insulating materials.
Gears 143 are fixed to both ends of the rotation shaft 141, and the gears 143 are located in the driving grooves 14. A slide rail 144 is fixed on one side in the driving groove 14, a rack 145 is slidably arranged in the slide rail 144, and the gears 143 are meshed with the rack 145.
The upper side of cultivate cabinet 1 is provided with lift groove 16, is provided with lifter plate 161 in the lift groove 16, and the upper end of rack 145 is all fixed with lifter plate 161. The telescopic rods 162 are fixed to the upper sides of the lifting grooves 16, and in this embodiment, the telescopic rods 162 are electric telescopic rods, and the output rods of the telescopic rods 162 are fixed to the lifting plates 161.
The first temperature detectors 36 are fixed in the sliding cabinet 3, and the second temperature detectors 17 are fixed in the cultivating cabinet 1. One side of the culture cabinet 1 is fixedly provided with a temperature control device 5, and the temperature control device 5 is communicated with the interior of the culture cabinet 1.
The temperature control device for cell culture further comprises a control system. The temperature control device 5, the control panel 2, the telescopic rod 162, the driver 124, the first temperature detector 36 and the second temperature detector 17 are all connected with a control system.
In the cell culture state, cabinet door 13 covers cabinet opening 11, and electric buckle 132 is fixed cabinet door 13, makes the inside airtight state that keeps of culture cabinet 1 to avoid culture cabinet 1 unexpected opening. The cover plate 4 is in an open state, so that the insides of the sliding cabinets 3 are communicated with the insides of the culture cabinets 1. Meanwhile, the temperature control device 5 is used for controlling the internal temperature of the culture cabinet 1, so that the slide cabinet 3 and the culture cabinet 1 are both at proper temperatures for cell culture.
The worker can set a desired incubation temperature through the control panel 2.
When a worker needs to access the cell culture container, the control system comprises the following operation steps:
S1, operating and opening a cabinet door 13 at a control panel 2, controlling a telescopic rod 162 to extend and then shorten by a control system, and unlocking an electric buckle 132;
in this step, the rack 145 is driven to move downward and then upward by the telescopic rod 162.
When the rack 145 moves downward, the gear 143 drives the rotating shaft 141 to rotate, and the rotating shaft 141 pushes the stop block 44 through the push block 142, so that the cover plate 4 turns upward to cover the opening of the accommodating groove 12, and the inside of the accommodating groove 12 can be kept sealed through the elasticity of the sealing ring 45.
Then, the rack 145 moves upwards, the rotating shaft 141 is driven to rotate reversely by the gear 143, the rotating shaft 141 drives the push block 142 to reset, and at this time, the push block 142 is separated from the stop block 44 by a certain distance, and the stop block 44 is not pushed reversely to open the cover plate 4. At this time, the cover plate 4 can be kept in a state of covering the accommodating groove 12 by the attraction between the first magnet 42 and the second magnet 125.
After that, the electric buckle 132 is opened, and the staff can open the cabinet door 13, so that the inside of the culture cabinet 1 is communicated with the outside. When the cabinet door 13 is opened, the cover plate 4 keeps the inside of the accommodating groove 12 sealed, so that the temperature change in the accommodating groove 12 can be avoided, and the change of the storage environment of the cells stored in the sliding cabinet 3 before and the influence on the cells in culture can be avoided.
S2, opening a cabinet door 13, selecting a sliding cabinet 3 needing to be continuously accessed and operated at the control panel 2, and controlling a driver 124 at a corresponding position to start by a control system, wherein the sliding cabinet 3 stretches out;
In this step, the driver 124 is configured to drive the driving rod 123 to rotate, and the sliding block 31 is screwed to the driving rod 123, so that the sliding block 31 drives the sliding cabinet 3 to extend out of the accommodating groove 12.
The sliding cabinet 3 can contact and squeeze the cover plate 4 in the extending process, and as the push block 142 is reset and separated from the stop block 44 by a certain distance, the cover plate 4 can be pushed open by the sliding cabinet 3, the cover plate 4 can open the accommodating groove 12, so that the sliding cabinet 3 extends out of the accommodating groove 12, and a worker can store a container for cell culture in the sliding cabinet 3.
When the shutter 33 is fully extended from the accommodating groove 12, a worker can operate the storage groove 35 deep inside the slide case 3 by lifting the shutter 33 using the cover plate groove 32.
If the operator only needs to operate the storage slot 35 at the opening of the sliding cabinet 3, the cover plate 4 can be opened directly by hand without selecting at the control panel 2.
S3, after the storage and the taking are completed, all the extended sliding cabinets 3 are controlled to reset at the control panel 2;
In this step, the control system controls the driving rods 123 at all extended sliding cabinets 3 to rotate reversely, and the sliding blocks 31 drive the sliding cabinets 3 to retract into the accommodating grooves 12 through the threaded connection of the driving rods 123 and the sliding blocks 31.
S4, after the sliding cabinet 3 is reset, closing the cabinet door 13, then operating the control panel 2 to close the cabinet door 13, and controlling the electric buckle 132 by the control system to lock the cabinet door 13;
S5, the control system respectively detects the temperature in each sliding cabinet 3 and the temperature in the culture cabinet 1 in real time through the first temperature detector 36 and the second temperature detector 17, and calculates the temperature difference T between the temperature in each sliding cabinet 3 and the set culture temperature and the temperature difference T between the temperature in the culture cabinet 1 and the set culture temperature;
when T is less than T, maintaining the state of each device at the position unchanged;
when T is larger than or equal to T, the control system controls the driver 124 at the corresponding position to rotate forward and then rotate reversely for resetting, and then stops detecting the temperature at the position.
In this step, because the cabinet door 13 is opened before, the temperature difference between the temperature in the culture cabinet 1 and the opened sliding cabinet 3 and the set culture temperature is large, after the sliding cabinet 3 is reset, the cover plate 4 is not closed, so that the opened sliding cabinet 3 is communicated with the interior of the culture cabinet 1, and the temperature can be controlled by the temperature control device 5 at the same time.
The unopened sliding cabinet 3 keeps airtight all the time, the temperature inside the sliding cabinet is relatively close to the set culture temperature, namely T is smaller than T, and the sliding cabinet 3 keeps airtight through the unchanged state of each device, so that the temperature inside the sliding cabinet is maintained, and the temperature difference between the temperature inside the sliding cabinet and the set culture temperature is prevented from being increased after the cabinet door 13 is opened, and the cell culture is prevented from being influenced.
Along with the continuous operation of the temperature control device 5, the temperature in the culture cabinet 1 gradually approaches the set culture temperature, so that T is reduced, when T is more than or equal to T, the temperature of the culture cabinet 1 is more close to the set culture temperature, the driver 124 rotates forward and then reversely rotates to reset, the sliding cabinet 3 stretches out a distance, the cover plate 4 is jacked up and then resets, the sliding cabinet 3 inside of the position is communicated with the culture cabinet 1, and the temperature control device 5 changes the temperature inside the sliding cabinet 3 after being opened to the set culture temperature.
After the cover plate 4 is arranged, the interior of the sliding cabinet 3 is communicated with the interior of the culture cabinet 1, so that the temperatures in the sliding cabinet 3 and the interior of the culture cabinet 1 are consistent, and the temperature in the culture cabinet 1 is only detected.
It will be apparent that the embodiments described above are merely some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that embodiments of the application described herein may be implemented in sequences other than those illustrated or otherwise described herein.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above examples, and all technical solutions belonging to the concept of the present invention belong to the protection scope of the present invention. It should be noted that modifications and adaptations to the present invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be within the scope of the present invention.
Claims (1)
1. A temperature control device for cell culture, characterized in that: including cultivateing cabinet (1), one side of cultivateing cabinet (1) is provided with cabinet mouth (11), cabinet mouth (11) inboard distribution has a plurality of holding tanks (12), one side rotation of cabinet mouth (11) is provided with cabinet door (13), the inside smooth cabinet (3) that all is provided with one side open-ended of holding tank (12), the opening part of smooth cabinet (3) is towards cabinet mouth (11), the opening part of holding tank (12) all is provided with apron (4), apron (4) are suitable for under the outside drive to cover or open the opening of holding tank (12), the bottom of smooth cabinet (3) inboard is provided with a plurality of storage tanks (35), one side fixed mounting of cultivateing cabinet (1) has temperature control device (5), temperature control device (5) and cultivate the intercommunication in cabinet (1).
The bottom of the accommodating groove (12) is provided with a sliding groove (121), a sliding block (31) is fixed at the lower side of the sliding cabinet (3), a plurality of sliding rods (122) are fixed between two ends of the sliding groove (121), the sliding rods (122) penetrate through the sliding block (31), the sliding rods (122) are in sliding connection with the sliding block (31), a driving rod (123) is rotationally connected between two ends of the sliding groove (121), a plurality of drivers (124) corresponding to the positions of the driving rod (123) are fixedly arranged on the outer wall of one side, far away from the cabinet opening (11), of the cultivating cabinet (1), an output shaft of each driver (124) is fixed with the corresponding driving rod (123), each driving rod (123) penetrates through the sliding block (31), and each driving rod (123) is in threaded connection with the corresponding sliding block (31).
The novel cabinet is characterized in that driving grooves (14) are formed in two sides of the cabinet opening (11), a plurality of rotating shafts (141) are connected between the driving grooves (14), exposing grooves (15) are formed in the lower sides of the accommodating grooves (12), the exposing grooves (15) are penetrated by the rotating shafts (141), cover plates (4) are arranged at openings of the accommodating grooves (12), shaft sleeves (41) are fixed on the lower sides of the cover plates (4), and the shaft sleeves (41) are rotationally sleeved on the rotating shafts (141) in the accommodating grooves (12);
The rotary shaft (141) is rotatably arranged between the driving grooves (14), a push block (142) corresponding to the shaft sleeve (41) is fixed on the rotary shaft (141), a push block hole (43) is formed in one end of the shaft sleeve (41), the push block (142) is positioned in the push block hole (43), and a stop block (44) is fixed on one side of the inner wall of the push block hole (43);
Gears (143) are fixed at two ends of the rotating shaft (141), the gears (143) are positioned in the driving grooves (14), a sliding rail (144) is fixed at one side in each driving groove (14), a rack (145) is arranged in the sliding rail (144) in a sliding manner, and the gears (143) are meshed with the racks (145);
The upper side of the culture cabinet (1) is provided with a lifting groove (16), a lifting plate (161) is arranged in the lifting groove (16), the upper ends of the racks (145) are all fixed with the lifting plate (161), the upper sides of the lifting groove (16) are all fixed with telescopic rods (162), and the output rods of the telescopic rods (162) are fixed with the lifting plate (161);
An observation plate (131) is fixed in the middle of the cabinet door (13), the observation plate (131) is made of a transparent material, an electric buckle (132) is connected between one side of the cabinet door (13) and the culture cabinet (1), a sealing ring (45) is fixed on one side of the cover plate (4) close to the accommodating groove (12), the sealing ring (45) is made of an elastic material, and the cover plate (4), the culture cabinet (1) and the cabinet door (13) are made of heat insulation materials;
A cover plate groove (32) is formed in the upper side of one end of the opening of the sliding cabinet (3), a baffle plate (33) is arranged in the cover plate groove (32), one end, far away from the cabinet opening (11), of the baffle plate (33) is rotatably connected with the inner wall of the cover plate groove (32), supporting blocks (34) are fixed at the bottoms of the two sides of the cover plate groove (32), and the supporting blocks (34) are positioned on the lower side of the baffle plate (32);
One side of cultivateing cabinet (1) is provided with control panel (2), the upside of apron (4) all is fixed with first magnetite (42), the upside of holding tank (12) is fixed with second magnetite (125) that correspond with first magnetite (42) position, all be fixed with first temperature detector (36) in smooth cabinet (3), cultivate cabinet (1) internal fixation has second temperature detector (17).
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| CN202410081393.7A CN117586871B (en) | 2024-01-19 | 2024-01-19 | Temperature control equipment for cell culture |
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| CN202410081393.7A CN117586871B (en) | 2024-01-19 | 2024-01-19 | Temperature control equipment for cell culture |
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| CN117586871A (en) | 2024-02-23 |
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