CN110791430A

CN110791430A - High-quality cow and sheep oocyte incubator based on embryo in vitro culture technology

Info

Publication number: CN110791430A
Application number: CN201911210161.2A
Authority: CN
Inventors: 邱寒峰; 章晓炜; 董信阳; 王邵雨; 王争光
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2019-12-02
Filing date: 2019-12-02
Publication date: 2020-02-14
Anticipated expiration: 2039-12-02
Also published as: JP6775725B1; CN110791430B; JP2021087415A

Abstract

The invention discloses a high-quality cattle and sheep oocyte incubator based on an embryo in-vitro culture technology, which comprises a case body, a culture plate and a bearing plate, wherein the culture plate is arranged in the case body, the bearing plate is arranged on the case body, a plurality of culture cavities are arranged on the case body, the culture plate is arranged in the culture cavities, a cavity matched with the bearing plate is arranged at the bottom of the case body, a first sliding groove is arranged on the side wall of the case body, a sliding block matched with the first sliding groove is arranged on the bearing plate, a second sliding groove matched with the first sliding groove is arranged on the inner wall of the cavity, a first through cavity is arranged on the bearing plate, a first baffle is arranged in the culture cavity, a second baffle is arranged in the first through cavity, and a through hole is arranged on the side wall; a plurality of culture boxes are detachably connected to the culture plate; the number of the culture boxes is selected according to the number of cells to be cultured actually, so that waste of the culture boxes is prevented, and the experiment cost is reduced.

Description

High-quality cow and sheep oocyte incubator based on embryo in vitro culture technology

Technical Field

The invention belongs to the technical field of cell culture devices, and particularly relates to a high-quality cow and sheep oocyte culture box based on an embryo in-vitro culture technology.

Background

With the progress of embryo engineering technology, embryo biotechnology is widely applied in more and more fields, and the development of many disciplines such as medicine, livestock raising, genetic engineering, animal protection and the like is promoted. In vitro culture is the core of embryo biotechnology, and both oocyte and embryo in vitro culture need to use culture vessels, and at present, culture vessels and culture plates are mainly used. The culture plate has a plurality of culture wells, typically 6, 12, 24, 48, 96 wells, and only one or a few culture wells are used in each batch of experiments because of concerns about increased probability of cell contamination, and the remaining culture wells on the culture plate are rarely reused, resulting in increased cost of the experiments. The existing culture dish is not suitable for in-vitro culture of few cells due to large volume, and the whole culture dish needs to be covered with paraffin oil, so that not only is space wasted, but also the paraffin oil is wasted; if cultivate multiunit cell in a culture dish, can avoid extravagant, but the culture liquid drop in the culture dish is nearer apart from each other, can rock because of the culture dish and lead to different processing liquid drops to mix, causes the experiment failure.

For guaranteeing cell survival rate, the culture dish need be arranged in the incubator usually and is cultivateed, and the environment in the box is suitable cell growth environment, has the difference with external environment, and the cell needs the incubator of opening regularly to be observed the cell in the process of cultivateing, and external air directly enters into in the box when the incubator is opened, causes the influence to the gas balance in the box to influence oocyte survival rate.

Disclosure of Invention

The invention provides a high-quality cow and sheep oocyte incubator based on an embryo in-vitro culture technology to overcome the defects of the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme: a high-quality cow and sheep oocyte incubator based on an embryo in-vitro culture technology comprises a box body, a culture plate and a receiving plate, wherein the culture plate is arranged in the box body, the receiving plate is arranged on the box body, a plurality of culture cavities are formed in the box body, the culture plate is arranged in the culture cavities, a cavity matched with the receiving plate is formed in the bottom of the box body, a first sliding groove is formed in the side wall of the box body, a sliding block matched with the first sliding groove is arranged on the receiving plate, a second sliding groove matched with the first sliding groove is formed in the inner wall of the cavity, a first through cavity is formed in the receiving plate, a first baffle is arranged in the culture cavity, a second baffle is arranged in the first through cavity, and a through hole is formed in the side wall of; a plurality of culture boxes are detachably connected to the culture plate; when the oocytes are cultured, the culture boxes are arranged on the culture plate according to the number of the oocytes to be cultured, so that the oocytes are cultured in the culture boxes, each culture box is utilized, the number of the culture boxes is selected according to the number of the cells to be cultured actually, the waste of the culture boxes is prevented, and the experiment cost is reduced; the cells are respectively placed in different culture boxes for culture, so that the culture solutions in the different culture boxes are prevented from being mixed with each other, and the culture survival rate of the oocytes is improved; when the culture plate in the formulated culture cavity is taken out, the bearing plate is pulled out from the cavity, the bearing plate is pushed to move along the first sliding groove, after the bearing plate is moved to one side of the specified culture cavity, the gas pipe is connected to the through hole, the gas environment in the first through cavity is adjusted, the same environment as that in the box body is formed in the first through cavity, the first baffle is opened, the culture plate is pushed into the first through cavity, the first baffle is closed after the culture plate completely enters the first through cavity, the second baffle is opened to take out the culture plate from the first through cavity, the situation that the external air directly enters the box body to change the environment in the box body when one culture plate is taken out is prevented, and the survival rate of oocytes in the box body is ensured; when the bearing plate is not used, the bearing plate enters the cavity and is contained through the cavity, so that the bearing plate is prevented from being hung on the side wall of the incubator body to influence the use convenience of the incubator.

The culture plate is provided with a plurality of first connecting grooves, and the bottom of the culture box is provided with a first connecting block matched with the first connecting grooves; a second through cavity is formed in the side wall of the culture cavity, a first push rod penetrates through the second through cavity, a first movable cavity is formed in the inner wall of the second through cavity, and a first limiting plate matched with the first movable cavity is arranged on the first push rod; under the mutual matching of the first connecting groove and the first connecting block, the culture box is fixed and fixed at a designated position on the culture plate, so that the culture box is prevented from moving on the culture plate to shake out the culture solution from the culture box; after the bearing plate moves to the side wall of the appointed culture cavity, pushing a first push rod into the culture cavity, and enabling the first push rod to push the culture plate to move towards the outer side of the culture cavity so as to push the culture plate out of the culture cavity; the first limiting plate limits the movement stroke of the first push rod, the first push rod is prevented from being separated from the first through cavity, and the connection effect of the first push rod and the box body is guaranteed.

A first connecting plate is arranged at one end of the first push rod, a third sliding groove is formed in the side wall of the first connecting plate, a movable block is arranged in the third sliding groove, a second connecting plate is rotatably connected to the movable block, and a first groove matched with the second connecting plate is formed in the side wall of the box body; when the first push rod is not used, the second connecting plate and the first push rod are in a vertical state, the second connecting plate is accommodated in the first groove, the situation that the incubator topples due to the fact that the second connecting plate is touched when the incubator is operated is avoided, and a stable environment is provided for cell culture; after the bearing plate moves to the side wall of the culture cavity, the first baffle is opened, the second connecting plate is rotated to enable the second connecting plate to rotate to be in a linear state with the first push rod, the second connecting plate is pushed into the culture cavity, the first push rod is pushed by the second connecting plate to enter the culture cavity, and the culture plate is pushed out from the culture cavity so as to be taken out of the culture plate in the specified culture cavity independently.

A second groove matched with the second connecting plate is formed in one end of the first push rod, a second movable cavity is formed in the top of the culture cavity, a third push rod penetrates through the second movable cavity, a third connecting plate is arranged at the top of the third push rod, and when the bearing plate moves to one side of the culture cavity, the first baffle and the third push rod move together; when the second connecting plate rotates to be in a linear state with the first push rod, the second connecting plate is pushed towards the first push rod, so that the second connecting plate is inserted into the second groove, and the second connecting plate is limited, so that stable pushing force is provided for the first push rod under the action of the second connecting plate; after the accepting plate moves to one side of the culture cavity, the third connecting plate is pulled upwards to drive the third push rod to move upwards, the third push rod is matched with the first baffle on the corresponding culture cavity, and the third push rod drives the first baffle to move upwards when moving upwards, so that the culture cavity is communicated with the first through cavity, and the situation that the outside air enters the box body when the culture plate in a certain culture cavity is taken alone is avoided.

A second connecting groove is formed in the side wall of the culture cavity, a third movable cavity is formed in the bearing plate, a fourth connecting plate matched with the second connecting groove is arranged in the third movable cavity, a movable groove is formed in the top of the third movable cavity, a fifth connecting plate is arranged on the fourth connecting plate, and a push block matched with the movable groove is arranged on the fifth connecting plate; when the culture plate in one of the culture cavities needs to be taken out, the bearing plate is pushed along the first sliding groove, the bearing plate is moved to one side of the appointed culture cavity, the second connecting groove is aligned with the third movable cavity, the pushing block is pushed to enable the fourth connecting plate to be inserted into the second connecting groove, the connecting part of the bearing plate and the box body is sealed, and air is prevented from entering the box body from the connecting part; under the mutually supporting of fourth connecting plate and second spread groove, play the fixed action to accepting the board, make and accept board and cultivate the chamber and form stable cooperation to take out the cultivation board in the chamber.

A fourth movable cavity is arranged on the first baffle, a third through cavity is arranged on the side wall of the fourth movable cavity, a fifth movable cavity is arranged on the side wall of the third through cavity, a connecting rod penetrates through the third through cavity, a second limiting plate matched with the fifth movable cavity is arranged on the connecting rod, a first reset spring is arranged on the second limiting plate, and a second push rod used for pushing the connecting rod to move is arranged in the fourth movable cavity; a third connecting groove matched with the connecting rod is formed in the third push rod; when the bearing plate moves to the side wall of the culture cavity, the fourth connecting plate is inserted into the second connecting groove, the bearing plate is fixed on the side wall of the culture cavity, the fourth connecting plate is matched with the second push rod, the second push rod pushes the connecting rod to move towards the outer side of the third through cavity, the connecting rod is inserted into the third connecting groove, the connecting rod is matched with the third connecting groove, and therefore the third push rod drives the first baffle plate to move together when moving, and the first baffle plate on the culture cavity is opened.

An arc-shaped elastic sheet is arranged in the fourth movable cavity, and the second push rod is arranged on one side of the arc-shaped elastic sheet; a sixth connecting plate is arranged at the bottom of the fourth connecting plate, and a third limiting plate matched with the second push rod is arranged on the sixth connecting plate; after accepting the board and removing to cultivateing chamber one side, promote the ejector pad toward the box direction, make the fourth connecting plate insert in the second spread groove, it removes to drive the sixth connecting plate when the second connecting plate removes, make the sixth connecting plate remove to second push rod one side, the sixth connecting plate promotes the second push rod and removes toward fourth movable cavity, the second push rod promotes arc shell fragment and takes place deformation, arc shell fragment both ends are toward extending outward, promote the connecting rod and move toward the third pass outside of the chamber, thereby make the connecting rod insert in the third spread groove, accomplish first baffle and the cooperation of being connected of third push rod.

A second connecting block is arranged at the bottom of the fourth connecting plate, a sixth movable cavity matched with the sixth connecting plate is arranged on the second connecting block, and a connecting spring is arranged on the sixth connecting plate; when the third push rod moves upwards, the first baffle is driven to move upwards, the first baffle drives the second push rod to move upwards, the second push rod is driven to move upwards under the action of the third limiting plate, the sixth connecting plate is driven to move upwards when the second push rod moves upwards, the sixth connecting plate is inserted into the sixth movable cavity, the sixth connecting plate is located at the top of the first through cavity, and the situation that the sixth connecting plate obstructs the movement of the culture plate is avoided.

A seventh movable cavity communicated with the third movable cavity is arranged on the bearing plate, a fourth through cavity is arranged on the side wall of the seventh movable cavity, an eighth movable cavity is arranged on the inner wall of the fourth through cavity, a fixed rod penetrates through the fourth through cavity, and a fixed groove matched with the fixed rod is arranged on the inner wall of the first sliding groove; when the fourth connecting plate moves, the fixing rod can be pushed to be inserted into the fixing groove; after the bearing plate moves to the side wall of the culture cavity, the pushing block is pushed to enable the fourth connecting plate to be inserted into the second connecting groove, the fixing rod is pushed to move when the fourth connecting plate moves, the fixing rod is enabled to be inserted into the fixing groove, under the mutual matching of the fixing rod and the fixing groove, supporting force is provided for the bearing plate, the fourth connecting plate is matched with the box body, the connection effect of the bearing plate and the box body is guaranteed, and the external air is prevented from entering the culture cavity when the first baffle is opened.

A fourth limiting plate matched with the eighth movable cavity is arranged on the fixed rod, a second reset spring is arranged on the fourth limiting plate, a seventh connecting plate is arranged on the side wall of the fourth connecting plate, and a push plate matched with the fixed rod is arranged on the seventh connecting plate; the bearing plate is provided with a connecting frame, and the connecting frame is provided with a limiting rod matched with the push block; the fourth connecting plate drives the push plate to move when moving, the push plate pushes the fixed rod to move towards the outer side of the fourth through cavity, the fixed rod is pushed into the fixed groove, the fixed plate is connected and matched with the fixed groove, and the bearing plate is fixed on the side wall of the box body; after the fourth connecting plate inserted in the second spread groove, promote the gag lever post, make the gag lever post move to ejector pad one side, the gag lever post provides the holding power for the ejector pad, fixes the ejector pad in activity groove one end, guarantees the cooperation effect of fourth connecting plate and second spread groove, promotes the connection effect who accepts board and box.

The invention has the following advantages: the number of the culture boxes is selected according to the number of cells to be cultured actually, so that the waste of the culture boxes is prevented, and the experiment cost is reduced; the cells are respectively placed in different culture boxes for culture, so that the culture solutions in the different culture boxes are prevented from being mixed with each other, and the culture survival rate of the oocytes is improved; under the board setting of accepting, prevent that the external air directly enters into the box when taking one of them culture plate and changes the internal environment of box, guarantee the interior oocyte survival rate of box.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a first cross-sectional view of the present invention.

Fig. 3 is an enlarged view of a portion a in fig. 2.

Fig. 4 is an enlarged view of fig. 2 at B.

Fig. 5 is an enlarged view of fig. 2 at C.

Fig. 6 is an enlarged view of fig. 2 at D.

FIG. 7 is a second cross-sectional view of the present invention.

Fig. 8 is an enlarged view of fig. 7 at E.

Fig. 9 is an enlarged view of fig. 7 at F.

Fig. 10 is a third schematic sectional view of the present invention.

Fig. 11 is an enlarged view at G in fig. 10.

Fig. 12 is an enlarged view of fig. 10 at H.

Fig. 13 is a fourth schematic cross-sectional view of the present invention.

Fig. 14 is an enlarged view at I in fig. 13.

Fig. 15 is an enlarged view at J in fig. 13.

Detailed Description

As shown in fig. 1-15, a high-quality cow and sheep oocyte incubator based on embryo in vitro culture technology comprises a case body 1, a culture plate 3 arranged in the case body 1 and a receiving plate 2 arranged on the case body 1, wherein the case body 1 is provided with a plurality of culture chambers, the culture plate 3 is arranged in the culture chambers, the bottom of the case body 1 is provided with a cavity matched with the receiving plate 2, the side wall of the case body 1 is provided with a first chute, the receiving plate 2 is provided with a slide block matched with the first chute, the inner wall of the cavity is provided with a second chute matched with the first chute, the receiving plate 2 is provided with a first through chamber, the culture chamber is provided with a first baffle 11, the first through chamber is provided with a second baffle 21, and the side wall of the first through chamber is provided with a through hole; a plurality of culture boxes 32 are detachably connected to the culture plate 3; when the oocytes are cultured, the culture boxes are arranged on the culture plate according to the number of the oocytes to be cultured, so that the oocytes are cultured in the culture boxes, each culture box is utilized, the number of the culture boxes is selected according to the number of the cells to be cultured actually, the waste of the culture boxes is prevented, and the experiment cost is reduced; the cells are respectively placed in different culture boxes for culture, so that the culture solutions in the different culture boxes are prevented from being mixed with each other, and the culture survival rate of the oocytes is improved; when the culture plate in the formulated culture cavity is taken out, the bearing plate is pulled out from the cavity, the bearing plate is pushed to move along the first sliding groove, after the bearing plate is moved to one side of the specified culture cavity, the gas pipe is connected to the through hole, the gas environment in the first through cavity is adjusted, the same environment as that in the box body is formed in the first through cavity, the first baffle is opened, the culture plate is pushed into the first through cavity, the first baffle is closed after the culture plate completely enters the first through cavity, the second baffle is opened to take out the culture plate from the first through cavity, the situation that the external air directly enters the box body to change the environment in the box body when one culture plate is taken out is prevented, and the survival rate of oocytes in the box body is ensured; when the bearing plate is not used, the bearing plate enters the cavity and is contained through the cavity, so that the bearing plate is prevented from being hung on the side wall of the incubator body to influence the use convenience of the incubator.

A plurality of first connecting grooves 31 are formed in the culture plate 3, and first connecting blocks matched with the first connecting grooves 31 are arranged at the bottom of the culture box 32; a second through cavity is formed in the side wall of the culture cavity, a first push rod 12 penetrates through the second through cavity, a first movable cavity is formed in the inner wall of the second through cavity, and a first limiting plate 121 matched with the first movable cavity is arranged on the first push rod 12; under the mutual matching of the first connecting groove and the first connecting block, the culture box is fixed and fixed at a designated position on the culture plate, so that the culture box is prevented from moving on the culture plate to shake out the culture solution from the culture box; after the bearing plate moves to the side wall of the appointed culture cavity, pushing a first push rod into the culture cavity, and enabling the first push rod to push the culture plate to move towards the outer side of the culture cavity so as to push the culture plate out of the culture cavity; the first limiting plate limits the movement stroke of the first push rod, the first push rod is prevented from being separated from the first through cavity, and the connection effect of the first push rod and the box body is guaranteed.

A first connecting plate 122 is arranged at one end of the first push rod 12, a third sliding groove is formed in the side wall of the first connecting plate 122, a movable block 123 is arranged in the third sliding groove, a supporting spring 124 is arranged at the bottom of the movable block, a second connecting plate 13 is rotatably connected to the movable block 123, and a first groove matched with the second connecting plate 13 is formed in the side wall of the box body 1; when the first push rod is not used, the second connecting plate and the first push rod are in a vertical state, the second connecting plate is accommodated in the first groove, the situation that the incubator topples due to the fact that the second connecting plate is touched when the incubator is operated is avoided, and a stable environment is provided for cell culture; after the bearing plate moves to the side wall of the culture cavity, the first baffle is opened, the second connecting plate is rotated to enable the second connecting plate to rotate to be in a linear state with the first push rod, the second connecting plate is pushed into the culture cavity, the first push rod is pushed by the second connecting plate to enter the culture cavity, and the culture plate is pushed out from the culture cavity so as to be taken out of the culture plate in the specified culture cavity independently.

A second groove matched with the second connecting plate 13 is formed in one end of the first push rod 12, a second movable cavity is formed in the top of the culture cavity, a third push rod 14 penetrates through the second movable cavity, a third connecting plate 141 is arranged at the top of the third push rod 14, and when the bearing plate 2 moves to one side of the culture cavity, the first baffle plate 11 and the third push rod 14 move together; when the second connecting plate rotates to be in a linear state with the first push rod, the second connecting plate is pushed towards the first push rod, so that the second connecting plate is inserted into the second groove, and the second connecting plate is limited, so that stable pushing force is provided for the first push rod under the action of the second connecting plate; after the accepting plate moves to one side of the culture cavity, the third connecting plate is pulled upwards to drive the third push rod to move upwards, the third push rod is matched with the first baffle on the corresponding culture cavity, and the third push rod drives the first baffle to move upwards when moving upwards, so that the culture cavity is communicated with the first through cavity, and the situation that the outside air enters the box body when the culture plate in a certain culture cavity is taken alone is avoided.

A second connecting groove is formed in the side wall of the culture cavity, a third movable cavity is formed in the bearing plate 2, a fourth connecting plate 22 matched with the second connecting groove is arranged in the third movable cavity, a movable groove is formed in the top of the third movable cavity, a fifth connecting plate is arranged on the fourth connecting plate 22, and a pushing block 221 matched with the movable groove is arranged on the fifth connecting plate; when the culture plate in one of the culture cavities needs to be taken out, the bearing plate is pushed along the first sliding groove, the bearing plate is moved to one side of the appointed culture cavity, the second connecting groove is aligned with the third movable cavity, the pushing block is pushed to enable the fourth connecting plate to be inserted into the second connecting groove, the connecting part of the bearing plate and the box body is sealed, and air is prevented from entering the box body from the connecting part; under the mutually supporting of fourth connecting plate and second spread groove, play the fixed action to accepting the board, make and accept board and cultivate the chamber and form stable cooperation to take out the cultivation board in the chamber.

A fourth movable cavity is arranged on the first baffle 11, a third through cavity is arranged on the side wall of the fourth movable cavity, a fifth movable cavity is arranged on the side wall of the third through cavity, a connecting rod 112 penetrates through the third through cavity, a second limiting plate 1121 which is matched with the fifth movable cavity is arranged on the connecting rod 112, a first return spring 1122 is arranged on the second limiting plate 1121, and a second push rod 114 which is used for pushing the connecting rod 112 to move is arranged in the fourth movable cavity; a third connecting groove matched with the connecting rod 112 is formed in the third push rod 13; when the bearing plate moves to the side wall of the culture cavity, the fourth connecting plate is inserted into the second connecting groove, the bearing plate is fixed on the side wall of the culture cavity, the fourth connecting plate is matched with the second push rod, the second push rod pushes the connecting rod to move towards the outer side of the third through cavity, the connecting rod is inserted into the third connecting groove, the connecting rod is matched with the third connecting groove, and therefore the third push rod drives the first baffle plate to move together when moving, and the first baffle plate on the culture cavity is opened.

An arc-shaped elastic sheet 113 is arranged in the fourth movable cavity, and the second push rod 114 is arranged on one side of the arc-shaped elastic sheet 113; a sixth connecting plate 24 is arranged at the bottom of the fourth connecting plate 22, and a third limiting plate 241 matched with the second push rod 114 is arranged on the sixth connecting plate 24; after accepting the board and removing to cultivateing chamber one side, promote the ejector pad toward the box direction, make the fourth connecting plate insert in the second spread groove, it removes to drive the sixth connecting plate when the second connecting plate removes, make the sixth connecting plate remove to second push rod one side, the sixth connecting plate promotes the second push rod and removes toward fourth movable cavity, the second push rod promotes arc shell fragment and takes place deformation, arc shell fragment both ends are toward extending outward, promote the connecting rod and move toward the third pass outside of the chamber, thereby make the connecting rod insert in the third spread groove, accomplish first baffle and the cooperation of being connected of third push rod.

A second connecting block 222 is arranged at the bottom of the fourth connecting plate 22, a sixth movable cavity matched with the sixth connecting plate 24 is arranged on the second connecting block 222, and a connecting spring is arranged on the sixth connecting plate 24; when the third push rod moves upwards, the first baffle is driven to move upwards, the first baffle drives the second push rod to move upwards, the second push rod is driven to move upwards under the action of the third limiting plate, the sixth connecting plate is driven to move upwards when the second push rod moves upwards, the sixth connecting plate is inserted into the sixth movable cavity, the sixth connecting plate is located at the top of the first through cavity, and the situation that the sixth connecting plate obstructs the movement of the culture plate is avoided.

A seventh movable cavity communicated with the third movable cavity is arranged on the bearing plate 2, a fourth through cavity is arranged on the side wall of the seventh movable cavity, an eighth movable cavity is arranged on the inner wall of the fourth through cavity, a fixed rod 25 penetrates through the fourth through cavity, and a fixed groove matched with the fixed rod 25 is arranged on the inner wall of the first sliding groove; the fourth connecting plate 22 can push the fixing rod 25 to be inserted into the fixing groove when moving; when the fourth connecting plate moves, the fixing rod can be pushed to be inserted into the fixing groove; after the bearing plate moves to the side wall of the culture cavity, the pushing block is pushed to enable the fourth connecting plate to be inserted into the second connecting groove, the fixing rod is pushed to move when the fourth connecting plate moves, the fixing rod is enabled to be inserted into the fixing groove, under the mutual matching of the fixing rod and the fixing groove, supporting force is provided for the bearing plate, the fourth connecting plate is matched with the box body, the connection effect of the bearing plate and the box body is guaranteed, and the external air is prevented from entering the culture cavity when the first baffle is opened.

A fourth limiting plate 251 matched with the eighth movable cavity is arranged on the fixed rod 25, a second return spring 252 is arranged on the fourth limiting plate 251, a seventh connecting plate is arranged on the side wall of the fourth connecting plate 22, and a push plate 223 matched with the fixed rod 25 is arranged on the seventh connecting plate; the bearing plate 2 is provided with a connecting frame 231, and the connecting frame 231 is provided with a limiting rod 23 matched with the pushing block 231; the fourth connecting plate drives the push plate to move when moving, the push plate pushes the fixed rod to move towards the outer side of the fourth through cavity, the fixed rod is pushed into the fixed groove, the fixed plate is connected and matched with the fixed groove, and the bearing plate is fixed on the side wall of the box body; after the fourth connecting plate inserted in the second spread groove, promote the gag lever post, make the gag lever post move to ejector pad one side, the gag lever post provides the holding power for the ejector pad, fixes the ejector pad in activity groove one end, guarantees the cooperation effect of fourth connecting plate and second spread groove, promotes the connection effect who accepts board and box.

The air delivery pipes of different air storage bottles can be connected at the through hole according to actual requirements so as to adjust the environment in the first through cavity; when a culture plate in one of the culture cavities needs to be taken out, the bearing plate is pulled out of the cavity, the bearing plate is pushed along the first sliding groove to move to the side wall of the appointed culture cavity, the push block is pushed to enable the fourth connecting plate to be inserted into the second connecting groove, the sixth connecting plate is pushed to move when the fourth connecting plate moves to enable the sixth connecting plate to push the second push rod to move towards the fourth movable cavity, and the connecting rod is inserted into the third connecting groove; push pedal promotes the dead lever and removes, make the dead lever insert in the fixed slot, it is fixed to accept the board, promote the gag lever post, make the gag lever post move to ejector pad one side, it is fixed with the ejector pad, the gas-supply pipe is toward first logical intracavity carrier gas, do the adjustment to the environment of first logical intracavity, up stimulate the third push rod, make the third push rod drive to correspond to cultivate the first baffle on the chamber and open, rotate the second connecting plate, make the second connecting plate rotate to with a first push rod rectilinear state, toward training intracavity promotion second connecting plate, release the culture plate from training the intracavity, make the culture plate enter into first logical intracavity, close first baffle, open the second baffle, take out the culture plate from first logical intracavity, so that do the observation to the cell on the culture plate, prevent that the external air from causing the influence to the box internal environment.

Claims

1. The utility model provides a high-quality cattle and sheep oocyte incubator based on embryo external culture technique, includes box (1), locates culture plate (3) in box (1) and locating accept board (2) on box (1), be equipped with a plurality of cultivation chambeies on box (1), culture plate (3) are located cultivate the intracavity, box (1) bottom be equipped with accept board (2) matched with cavity, its characterized in that: a first sliding groove is formed in the side wall of the box body (1), a sliding block matched with the first sliding groove is arranged on the bearing plate (2), a second sliding groove matched with the first sliding groove is formed in the inner wall of the cavity, a first through cavity is formed in the bearing plate (2), a first baffle (11) is arranged in the culture cavity, a second baffle (21) is arranged in the first through cavity, and a through hole is formed in the side wall of the first through cavity; a plurality of culture boxes (32) are detachably connected to the culture plate (3); a plurality of first connecting grooves (31) are formed in the culture plate (3), and first connecting blocks matched with the first connecting grooves (31) are arranged at the bottom of the culture box (32); cultivate and be equipped with the second on the chamber lateral wall and lead to the chamber, first push rod (12) are worn to be equipped with in the second leads to the intracavity, be equipped with first activity chamber on the second leads to the intracavity wall, be equipped with on first push rod (12) with first activity chamber matched with first limiting plate (121).

2. The high-quality cow and sheep oocyte incubator based on the embryo in vitro culture technology according to claim 1, is characterized in that: first push rod (12) one end is equipped with first connecting plate (122), be equipped with the third spout on first connecting plate (122) lateral wall, be equipped with movable block (123) in the third spout, rotatable coupling has second connecting plate (13) on movable block (123), be equipped with on box (1) lateral wall with second connecting plate (13) matched with first recess.

3. The high-quality cow and sheep oocyte incubator based on the embryo in vitro culture technology according to claim 2, is characterized in that: a second groove matched with the second connecting plate (13) is formed in one end of the first push rod (12), a second movable cavity is formed in the top of the culture cavity, a third push rod (14) penetrates through the second movable cavity, a third connecting plate (141) is arranged at the top of the third push rod (14), and when the bearing plate (2) moves to one side of the culture cavity, the first baffle plate (11) and the third push rod (14) move together; the culture cavity is characterized in that a second connecting groove is formed in the side wall of the culture cavity, a third movable cavity is formed in the bearing plate (2), a fourth connecting plate (22) matched with the second connecting groove is arranged in the third movable cavity, a movable groove is formed in the top of the third movable cavity, a fifth connecting plate is arranged on the fourth connecting plate (22), and a pushing block (221) matched with the movable groove is arranged on the fifth connecting plate.

4. The high-quality cow and sheep oocyte incubator based on the embryo in vitro culture technology according to claim 3, is characterized in that: a fourth movable cavity is arranged on the first baffle (11), a third through cavity is arranged on the side wall of the fourth movable cavity, a fifth movable cavity is arranged on the side wall of the third through cavity, a connecting rod (112) penetrates through the third through cavity, a second limiting plate (1121) matched with the fifth movable cavity is arranged on the connecting rod (112), a first return spring (1122) is arranged on the second limiting plate (1121), and a second push rod (114) used for pushing the connecting rod (112) to move is arranged in the fourth movable cavity; a third connecting groove matched with the connecting rod (112) is formed in the third push rod (13); an arc-shaped elastic sheet (113) is arranged in the fourth movable cavity, and the second push rod (114) is arranged on one side of the arc-shaped elastic sheet (113); a sixth connecting plate (24) is arranged at the bottom of the fourth connecting plate (22), and a third limiting plate (241) matched with the second push rod (114) is arranged on the sixth connecting plate (24); a second connecting block (222) is arranged at the bottom of the fourth connecting plate (22), a sixth movable cavity matched with the sixth connecting plate (24) is arranged on the second connecting block (222), and a connecting spring is arranged on the sixth connecting plate (24).