CN215440490U

CN215440490U - Embryo operation and culture dish

Info

Publication number: CN215440490U
Application number: CN202121683189.0U
Authority: CN
Inventors: 刘欣; 张景程; 王德宝; 张和煦; 孙羽
Original assignee: Northwest A&F University
Current assignee: Northwest A&F University
Priority date: 2021-07-23
Filing date: 2021-07-23
Publication date: 2022-01-07
Anticipated expiration: 2031-07-23

Abstract

The utility model discloses an embryo handling and culture dish, which comprises a dish bottom, wherein a culture dish is arranged inside the dish bottom; the rubber ring groove is arranged on the periphery of one side of the outer part of the dish bottom; the rubber extrusion hole is arranged below the dish bottom; the dish cover is fixed below the rubber extrusion hole; the dish lid includes: the convex ring is arranged outside the dish cover; and the groove ring is arranged inside the convex ring. According to the embryo manipulation and culture dish, a culture dish with a semicircular structure is fixedly connected to the inside of the bottom of the dish, a common culture dish is used for embryo manipulation and change culture, and in the using process, the culture dish with the semicircular structure is adopted, and an embryo is manipulated by an egg picking needle; can make at the bottom of the culture dish can be covered to too little liquid, under the condition that embryo quantity is few, can practice thrift culture solution or operating fluid, satisfy that operating process liquid is few, the experiment of getting the ovum more, like parthenogenesis activation, can be fine satisfy.

Description

Embryo operation and culture dish

Technical Field

The utility model relates to the technical field of embryo manipulation and culture dishes, in particular to an embryo manipulation and culture dish.

Background

With the rapid development of the technological level, embryo engineering refers to various micromanipulation and processing techniques for early embryos or gametes of animals, including in vitro fertilization, embryo transplantation, embryo segmentation and transplantation, embryonic stem cell culture and other techniques. Maturation of immature oocytes and early in vitro culture of embryos are key requirements in early embryo development. The mature culture of the immature oocyte means that the immature cumulus-oocyte is placed in a maturation solution and cultured in a constant temperature CO2 incubator for a period of time to be converted into a mature state. In vitro embryo culture refers to placing fertilized embryos or parthenogenetic embryos in an embryo culture solution, culturing in a constant-temperature carbon dioxide incubator to enable the embryos to continuously develop until blastula are formed, and internationally, the embryos are generally cultured by adopting a microdroplet culture solution or a small-hole culture solution, and mineral oil covers the upper layer of the microdroplet culture solution or the small-hole culture solution.

In the embryo operation and culture dish using process in the market, the cylindrical culture dish structure has inconvenience in the embryo operation process by using the egg picking needle; the requirement of operations of taking more eggs with less liquid, such as parthenogenetic activation, cannot be met well; culture solution or operating fluid can not be well saved under the condition of small number of embryos, and too little liquid can not cover the bottom of the dish, so that the micro-operation process is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an embryo operation and culture dish, which aims to solve the problem that the cylindrical culture dish structure is inconvenient in the embryo operation process by using an egg picking needle in the embryo operation and culture dish using processes in the market proposed in the background art; the requirement of operations of taking more eggs with less liquid, such as parthenogenetic activation, cannot be met well; the culture solution or the operation solution can not be well saved under the condition of small number of embryos, and the bottom of the dish can not be covered by too little liquid, so that the micro-operation process is influenced.

In order to achieve the purpose, the utility model provides the following technical scheme: an embryo handling and culture dish comprising:

the culture dish is arranged inside the dish bottom;

the rubber ring groove is arranged on the periphery of one side of the outer part of the dish bottom;

the rubber extrusion hole is arranged below the dish bottom;

the dish cover is fixed below the rubber extrusion hole;

the dish lid includes:

the convex ring is arranged outside the dish cover;

and the groove ring is arranged inside the convex ring.

Preferably, the dish bottom is fixedly connected with the culture dish, and the culture dish is of a semicircular structure.

Preferably, the dish bottom and the rubber ring groove are of an integrated structure, and the dish bottom is in a concave disc shape.

Preferably, the utensil bottom is movably connected with the rubber extrusion hole, and the utensil bottom and the utensil cover form a clamping structure through the rubber extrusion hole.

Preferably, the rubber ring groove is movably connected with the dish cover, and the rubber ring groove and the dish bottom form an elastic structure through the dish cover.

Preferably, the periphery of the inner part of the raised ring is provided with a groove ring, and the shape and structure of the groove ring are matched with those of the rubber ring groove.

Compared with the prior art, the utility model has the beneficial effects that: the embryo manipulation and culture dish has the following beneficial effects: changing a common culture dish, and adopting a culture dish with a semicircular structure in the use process, wherein an embryo is operated by using an egg picking needle; can make too little liquid can cover the culture dish bottom, under the condition that embryo quantity is few, can practice thrift culture solution or operating fluid, satisfy that operating process liquid is few, the experiment of getting the ovum more, for example parthenogenetic activation.

1. According to the utility model, the concave structure of the inner wall of the dish bottom is designed, so that when the handheld egg picking needle is used, the side close to the hand can well suck embryos; meanwhile, the concave inner wall can enable the egg picking needle to abut against the inner wall, so that the embryo or the oocyte is remained in the needle, and the liquid flows out; the dish bottom can be covered with less culture solution when the embryo is cultured with less eggs, and less incubation solution can be used in the antibody incubation.

2. According to the utility model, when the dish cover is movably clamped on the dish bottom through the rubber extrusion hole, the dish cover and the groove ring in the convex ring are elastically clamped on the rubber ring groove with the shape and the structure matched with each other, the rubber extrusion hole can be extruded between the dish bottom and the dish cover, air can be fed through the rubber extrusion hole, such as carbon dioxide, so that the pH value of a culture solution is maintained, and meanwhile, the inner wall of the dish bottom is tangent to the table top, so that the temperature of the liquid in the dish is kept as constant as possible when the dish is placed on a hot table; the independent use also reduces the waste of the existing combined dish, and the dish cover is half of the dish bottom and is convenient to take.

Drawings

FIG. 1 is a schematic front view of the present invention;

FIG. 2 is a schematic view of the combined enclosure structure of the present invention;

FIG. 3 is a left side view of the present invention.

In the figure: 1. a dish bottom; 2. a culture dish; 3. a rubber ring groove; 4. a rubber extrusion hole; 5. a dish cover; 501. a raised ring; 502. a groove ring.

Detailed Description

As shown in fig. 1-2, the present invention provides a technical solution: an embryo handling and culture dish comprising: a culture dish 2 is arranged inside the dish bottom 1; the rubber ring groove 3 is arranged on the periphery of one side of the outside of the dish bottom 1; the rubber extrusion hole 4 is arranged below the dish bottom 1; a dish cover 5 fixed below the rubber extrusion hole 4; the capsule 5 comprises: a raised ring 501 provided outside the dish lid 5; the concave groove ring 502 is arranged inside the convex ring 501, the dish bottom 1 is fixedly connected with the culture dish 2, the culture dish 2 is of a semicircular structure, the culture dish 2 of the semicircular structure is fixedly connected inside the dish bottom 1, common culture dishes for embryo operation and change culture are arranged, and in the using process, the culture dish 2 of the semicircular structure is arranged in the embryo operation process by using the ovum picking needle; the culture dish 2 is covered by too little liquid, culture liquid or operating liquid can be well saved under the condition of small embryo quantity, operation requiring less liquid and more eggs can be well met, such as parthenogenetic activation, the dish bottom 1 and the rubber ring groove 3 are of an integrated structure, the dish bottom 1 is of a concave disc shape, the culture dish 2 is fixed on the dish bottom 1 and the rubber ring groove 3 of the integrated structure, the concave structure of the inner wall of the dish bottom 1 is designed, and when a handheld egg picking needle is used, one side close to a hand can well suck embryos; meanwhile, the concave inner wall can enable the egg picking needle to abut against the inner wall, so that the embryo or the oocyte is remained in the needle, and the liquid flows out; when a small number of eggs are cultured on the embryo, a small number of culture solution can be used for covering the dish bottom 1, a small number of incubation solution can be used for antibody incubation, the dish bottom 1 is movably connected with the rubber extrusion hole 4, the dish bottom 1 and the dish cover 5 form a clamping structure through the rubber extrusion hole 4, the soft rubber extrusion hole 4 can move along the dish bottom 1, the dish cover 5 can be movably clamped along the dish bottom 1 through the rubber extrusion hole 4, the rubber ring groove 3 is movably connected with the dish cover 5, and the rubber ring groove 3 and the dish bottom 1 form an elastic structure through the dish cover 5, when the culture dish 2 is fixed on the dish bottom 1 and the rubber ring groove 3 which are in an integrated structure, the dish cover 5 can be movably clamped on the dish bottom 1 through the rubber extrusion hole 4, and the inner part of the dish cover 5 can be extruded by the elasticity of the rubber ring groove 3, so that the dish cover 5 is elastically clamped on the dish bottom 1 and the rubber ring groove 3.

As shown in fig. 3, the present invention provides a technical solution: a kind of embryo operates and culture dish, the inner periphery of the protruding ring 501 has concave groove ring 502, and the shape structure of the concave groove ring 502 is identical to the shape structure of the rubber ring groove 3, connect the protruding ring 501 to the dish lid 5 fixedly, can make the concave groove ring 502 open in the protruding ring 501, when the dish lid 5 is through the rubber extrusion hole 4 along the flexible block on the dish bottom 1, make the trough ring 502 elasticity block in the dish lid 5 and the protruding ring 501 on the rubber ring groove 3 identical in shape structure, the rubber extrusion hole 4 can be squeezed between dish bottom 1 and dish lid 5, can admit air through the rubber extrusion hole 4, such as carbon dioxide, maintain the pH value of the culture solution, the tangent of inner wall and mesa of the dish bottom 1 has guaranteed the operating dish is put in the hot stage too the liquid temperature as possible at the same time too; the independent use also reduces the waste of the existing combined dish, and the dish cover 5 is half of the dish bottom 1 and is convenient to take.

In conclusion, when the embryo manipulation and culture dish is used, the soft rubber extrusion hole 4 can move along the dish bottom 1, the dish cover 5 can be movably clamped along the dish bottom 1 through the rubber extrusion hole 4, the convex ring 501 is fixedly connected to the dish cover 5, the rubber extrusion hole 4 can be extruded between the dish bottom 1 and the dish cover 5, air can be fed through the rubber extrusion hole 4, such as carbon dioxide, the pH value of a culture solution is maintained, the groove ring 502 can be arranged inside the convex ring 501, when the dish cover 5 is movably clamped along the dish bottom 1 through the rubber extrusion hole 4, the groove ring 502 in the dish cover 5 and the convex ring 501 can be elastically clamped on the rubber ring groove 3 with the matched shape structure, and meanwhile, the inner wall of the dish bottom 1 is tangent to the table top, so that the temperature of the liquid inside the manipulation dish is kept as constant as possible when the manipulation dish is placed on the hot table; the independent use also reduces the waste of the existing combined dish, the dish cover 5 is half of the height of the dish bottom 1 and is convenient to take, the culture dish 2 is fixed on the dish bottom 1 and the rubber ring groove 3 which are of an integrated structure, the concave structure of the inner wall of the dish bottom 1 is designed, and when the handheld egg picking needle is used, one side close to the hand can well suck embryos; meanwhile, the egg picking needle can be abutted against the inner wall by the concave inner wall, so that an embryo or an oocyte is remained in the needle, liquid flows out, the culture dish 2 in a semicircular structure is fixedly connected inside the dish bottom 1, the common culture dish 2 in the semicircular structure is generally used for embryo operation and change culture, and in the using process, the culture dish 2 in the semicircular structure is used for the embryo operation process by the egg picking needle; too little liquid can cover the bottom of the culture dish 2, and the culture solution or the operating solution can be well saved under the condition of small number of embryos.

Claims

1. An embryo handling and culture dish, comprising:

the culture dish is characterized by comprising a dish bottom (1), wherein a culture dish (2) is arranged inside the dish bottom (1);

the rubber ring groove (3) is arranged on the periphery of one side of the outside of the dish bottom (1);

the rubber extrusion hole (4) is arranged below the dish bottom (1);

a dish cover (5) fixed below the rubber extrusion hole (4);

the capsule (5) comprises:

a raised ring (501) arranged outside the dish cover (5);

a groove ring (502) that opens inside the raised ring (501).

2. The embryo handling and culture dish of claim 1, wherein: the dish bottom (1) is fixedly connected with the culture dish (2), and the culture dish (2) is of a semicircular structure.

3. The embryo handling and culture dish of claim 1, wherein: the dish bottom (1) and the rubber ring groove (3) are of an integrated structure, and the dish bottom (1) is of a concave disc shape.

4. The embryo handling and culture dish of claim 1, wherein: the utensil bottom (1) is movably connected with the rubber extrusion hole (4), and the utensil bottom (1) and the utensil cover (5) form a clamping structure through the rubber extrusion hole (4).

5. The embryo handling and culture dish of claim 1, wherein: the rubber ring groove (3) is movably connected with the dish cover (5), and the rubber ring groove (3) and the dish bottom (1) form an elastic structure through the dish cover (5).

6. The embryo handling and culture dish of claim 1, wherein: the periphery of the inner part of the raised ring (501) is provided with a groove ring (502), and the shape structure of the groove ring (502) is matched with that of the rubber ring groove (3).