CN115029241A - Multi-functional intelligent cell culture dish - Google Patents
Multi-functional intelligent cell culture dish Download PDFInfo
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- CN115029241A CN115029241A CN202210601062.2A CN202210601062A CN115029241A CN 115029241 A CN115029241 A CN 115029241A CN 202210601062 A CN202210601062 A CN 202210601062A CN 115029241 A CN115029241 A CN 115029241A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
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Abstract
The invention relates to the technical field of cell culture equipment, in particular to a multifunctional intelligent cell culture dish, which comprises a plurality of culture dishes, wherein each culture dish comprises a dish support and a dish cover capable of being buckled on the dish support, the outer wall of the dish support is a convex surface, the bottom end of the inner side of the dish cover is a concave surface, and the convex surface and the concave surface can amplify images in the dish support through refracted rays so as to be convenient for observation; the upper end of the outer wall of the dish support is provided with an external thread, the inner side of the dish cover is provided with an internal thread, and the dish cover is screwed on the external thread of the dish support through the internal thread; the upper end of the inner wall of the dish support is provided with an annular arc edge, the middle part of the dish support is provided with an inner pipe, and the arc edge and the concave surface are positioned on the same radian line; the image of the interior of the culture dish can be magnified for convenient observation.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to a multifunctional intelligent cell culture dish.
Background
A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant material, microorganism culture and animal cell adherent culture. The plastic may be a polyethylene material, disposable and reusable;
but the lateral wall and the lid of current culture dish are smooth surface, when observing the inside condition of culture dish through the culture dish, can only see the proportion and be the inside image of culture dish of actual size, and the inside image of culture dish can't enlarge is convenient for observe.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the multifunctional intelligent cell culture dish, which can amplify images in the culture dish to facilitate observation.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the utility model provides a multi-functional intelligent cell culture dish, includes a plurality of culture dishes, and the culture dish includes that the ware holds in the palm, and can the lock holds in the palm the ware lid on the ware holds in the palm, and the outer wall that the ware held in the palm is the convex surface, and the inboard bottom of ware lid is the concave surface, and convex surface and concave surface can enlarge the image that the ware held in the palm in the middle of through refraction light.
Preferably, the upper end of the outer wall of the dish support is provided with an external thread, the inner side of the dish cover is provided with an internal thread, and the dish cover is screwed on the external thread of the dish support through the internal thread.
Preferably, the upper end of the inner wall of the dish support is provided with an annular arc edge, the middle of the dish support is provided with an inner pipe, and the arc edge and the concave surface are positioned on the same radian line.
Preferably, a rotating rod capable of stirring the cells and the culture solution in the culture dish is rotated in the inner tube.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic structural diagram of a multifunctional intelligent cell culture dish according to the present invention;
FIG. 2 is a schematic view of the convex surface and the convex ring of the present invention;
FIG. 3 is a schematic view of the structure of the arc edge and the rotating rod of the present invention;
FIG. 4 is a schematic structural view of the inner tube of the present invention;
FIG. 5 is a schematic view of the interior of the dish support of the present invention;
FIG. 6 is a schematic view of the concave and curved sides of the present invention;
FIG. 7 is a schematic view of the construction of the trough plate and the pressure plate of the present invention;
FIG. 8 is a schematic structural view of the springboard of the present invention;
FIG. 9 is a schematic view of the structure of the lamp, the shaft and the sleeve of the present invention;
fig. 10 is a schematic view of the structure of the swivel ring and the friction ring of the present invention.
Detailed Description
An exemplary procedure that may be derived from the illustrations shown in figures 1-6 to facilitate the operator's observation of the cell culture is:
the side wall and the cover of the existing culture dish are flat surfaces, when the internal condition of the culture dish is observed through the culture dish, only an internal image of the culture dish with the actual proportion can be seen, and the internal image of the culture dish cannot be amplified to facilitate observation; the multifunctional intelligent cell culture dish comprises a plurality of culture dishes made of glass materials, each culture dish comprises a dish support 01 and a dish cover 05 capable of being buckled on the dish support 01, the outer wall of the dish support 01 is a convex surface 02, the bottom end of the inner side of the dish cover 05 is a concave surface 08, and the convex surface 02 and the concave surface 08 can magnify images in the dish support 01 through refracted light rays so as to be observed conveniently; when the cell culture dish is used, cells and culture solution required by the cells are added into the dish support 01, then the dish cover 05 is covered, after the cells are cultured for a period of time, the interior of the dish support 01 can be observed from the side, and at the moment, image light inside the dish support 01 is refracted under the action of the convex surface 02, so that an operator can observe an image with the volume larger than the actual volume when observing from the outer side, and the operator can observe the condition and the image in the dish support 01 conveniently; similarly, the concave surface 08 allows the image and the condition of the dish holder 01 to be magnified and observed when the dish holder 05 is viewed from above.
An exemplary operation that can be achieved by observing fig. 3 to 6 to enhance the degree of coupling between the dish holder 01 and the dish lid 05 according to the contents shown in the figures is:
the common culture dish is characterized in that a cover is simply covered on a dish body and connected through a simple slot, when the culture dish is overturned, cells and culture solution are easy to spill out after the culture dish is mutually opened, an external thread is arranged at the upper end of the outer wall of a dish support 01 in the culture dish, an internal thread is arranged on the inner side of a dish cover 05, and the dish cover 05 is screwed on the external thread of the dish support 01 through the internal thread; through the mode of screwing up each other, strengthen the dish lid 05 and hold in the palm 01 joint strength with the dish, avoid when the culture dish turnover the dish lid 05 to drop from the dish holds in the palm 01 to avoid the dish to hold in the palm 01 inside cell and the culture solution spill.
One exemplary operation that can be obtained from the illustration of fig. 2 and 6 that allows the exchange of air between the interior of the culture dish and the outside is:
the inner tube 04 is formed in the middle of the dish support 01, the inner tube 04 can communicate the inside of the culture dish with the outside from the lower part, so that air can be smoothly exchanged and flowed, and oxygen required by cells in the culture dish can be provided in the culture process, so that the cells can grow conveniently;
meanwhile, the annular arc edge 03 is arranged at the upper end of the inner wall of the dish holder 01, the concave surface 08 is arranged at the bottom end of the inner side of the dish cover 05, when the culture solution flows due to shaking of the culture dish, the culture solution flows onto the concave surface 08 of the dish cover 05 under the guidance of the arc edge 03, and when the culture solution is contaminated by the arc edge 03 and the concave surface 08, the culture solution flows back to the dish holder 01 downwards along the surfaces of the concave surface 08 and the arc edge 03 by utilizing the gravity of the culture solution, so that the problem that the contaminated culture solution cannot flow down due to the plane structure of the cover of the traditional culture dish after being contaminated by the culture solution is avoided;
simultaneously when not covering dish lid 05, when the culture dish takes place to rock, arc limit 03 can hold in the palm 01 centre to the culture solution guide that rocks, avoids the culture solution to spill out from the lateral wall that the dish held in the palm 01.
An exemplary procedure for facilitating the handling of a culture dish according to what is shown in the figures is obtained by observing figures 2 to 6:
the outer wall of a common culture dish is smooth, when an operator takes up the culture dish from the side by using hands, the culture dish is easy to fall off due to slipping, and a convex ring 09 which can be conveniently held is formed on the outer wall of the dish support 01 in the application; when holding the dish support 01, the friction force can be increased through the relative action of the convex ring 09 on the outer wall of the dish support 01 and the hand, so that the dish support 01 is prevented from sliding off.
One exemplary operation that can be obtained by observing fig. 2 to 6 to stir the culture solution in the culture dish according to the contents shown in the figures is:
the culture solution in the culture dish is stirred by a glass rod in a common mode, the culture solution can adhere to the glass rod after the stirring is finished, so that the culture solution in the culture dish is lost, and a rotating rod 06 capable of stirring the cells and the culture solution in the culture dish is rotated in an inner tube 04; when needs stir the culture solution, only need use the finger to stir bull stick 06 and take place to rotate in inner tube 04, alright in order to make bull stick 06 rotatory around the axis of inner tube 04, stir the culture solution to make the culture solution remain throughout in the middle of the dish holds in the palm 01, avoid the loss of culture solution.
An exemplary operation that can be achieved by observing fig. 1 and 7 to stably place the culture dish according to the contents shown in the figures is:
the culture dish also comprises a groove plate 10 formed with a circular recess, the dish support 01 can be embedded in the circular recess and can slide on the groove plate 10 to press a pressure plate 11 of the dish cover 05, and a tension spring is connected between the pressure plate 11 and the groove plate 10; when depositing the culture dish, only need to screw up the ware lid 05 on ware support 01, then hold in the palm the circular sunken in the middle of 01 places the dish on frid 10, circular sunken side can hold in the palm 01 below card with the ware and advance, avoid the ware to hold in the palm 01 unexpected slip that takes place the horizontal direction on frid 10, then pull up clamp plate 11, the extension spring is stretched this moment, then rotate clamp plate 11, make clamp plate 11 move ware lid 05 top, loosen clamp plate 11 afterwards, can pull down clamp plate 11 when the extension spring resets, make clamp plate 11 push down in ware lid 05 top, thereby utilize clamp plate 11 and frid 10's cooperation to press from both sides ware lid 05 and ware support 01 tightly, avoid the unexpected slip of taking place in the time of depositing of ware lid 05 and ware support 01.
An exemplary operation that can be obtained by observing fig. 7 to 9 to agitate the culture solution at any time according to the contents shown in the drawings is:
the culture dish also comprises a sleeve 15 fixed in the middle of the circular recess and a rotating shaft 14 rotating in the sleeve 15, a rectangular block is formed on the rotating shaft 14, and a rectangular groove for inserting the rectangular block is formed at the lower end of the rotating rod 06; when placing the dish support 01 in the middle of the circular recess, along with the decline of dish support 01, inner tube 04 can cover on sleeve 15, make pivot 14 enter into in the middle of the inner tube 04, thereby make the rectangular block on the pivot 14 insert in the middle of the rectangular channel of bull stick 06 lower extreme, thereby when depositing, also can rotate pivot 14 and drive bull stick 06 and take place to rotate in dish support 01 is inside, stir the culture solution, thereby can be nimble stir the culture solution according to the growth degree of cell, so that make the cell distribute more evenly in the culture solution.
An exemplary operation that can be achieved by observing fig. 7 to 9 to supplement the light according to the contents shown in the figures is:
a lamp 13 is fixed on the sleeve 15, and the lamp 13 is provided with a power supply; when observing that the ware holds in the palm 01 inside, alright in order to turn on lamp 13, make the light of lamp 13 shine in the cell and the culture solution of ware support 01 through inner tube 04 to be convenient for operating personnel from the outside observation, can carry out light supplementation for the growth environment of cell under the not good condition of light simultaneously, the cell of being convenient for carries out photosynthesis, can be used for simulating the illumination environment.
An exemplary procedure that can be followed from the graph shown in fig. 8 and 10 to shake culture cells is:
the multifunctional intelligent cell culture dish further comprises a base 16 and a rotating ring 17 which rotates on the base 16, wherein a friction ring 18 is fixed on the rotating ring 17, a motor is installed on the base 16, a friction wheel 19 in friction transmission with the friction ring 18 is driven by the motor, and the groove plates 10 in the plurality of culture dishes are all fixed on the rotating ring 17; the multifunctional intelligent cell culture dish also comprises a flange 07 fixed on a base 16, and an elastic plate 12 made of elastic material is fixed at the bottom end of the groove plate 10; in the process of cultivation, can use the motor to drive the friction wheel 19 rotatory, make friction wheel 19 transmission friction ring 18 drive swivel 17 and take place rotatoryly around the axis of base 16 on base 16, can drive a plurality of frid 10 when swivel 17 is rotatory and rotate, can take place crooked when the flange 07 is touched to the diving board 12 on the frid 10, diving board 12 carries out the power of accumulation at crooked in-process simultaneously, when diving board 12 crosses flange 07, diving board 12 is because the elasticity of self is kick-backed, and rock in continuous emergence, thereby produce vibrations through rocking of diving board 12, thereby make the ware support 01 on the frid 10 take place vibrations, finally realize the shock culture to the cell.
Claims (10)
1. The utility model provides a multi-functional intelligent cell culture dish which characterized in that: including a plurality of culture dishes, the culture dish includes that the dish holds in the palm (01), and can the lock holds in the palm dish lid (05) on dish holds in the palm (01), and the outer wall that the dish held in the palm (01) is convex surface (02), and the inboard bottom of dish lid (05) is concave surface (08), and convex surface (02) and concave surface (08) can enlarge the image in the middle of dish holds in the palm (01) through refraction light.
2. The multifunctional intelligent cell culture dish of claim 1, wherein: the dish support is characterized in that an external thread is arranged at the upper end of the outer wall of the dish support (01), an internal thread is arranged on the inner side of the dish cover (05), and the dish cover (05) is screwed on the external thread of the dish support (01) through the internal thread.
3. The multifunctional intelligent cell culture dish of claim 1, wherein: the upper end of the inner wall of the dish support (01) is provided with an annular arc edge (03), the middle of the dish support (01) is provided with an inner pipe (04), and the arc edge (03) and the concave surface (08) are positioned on the same radian line.
4. The multifunctional intelligent cell culture dish of claim 1, wherein: the outer wall of the dish support (01) is provided with a convex ring (09) which can be held conveniently.
5. The multifunctional intelligent cell culture dish of claim 3, wherein: a rotating rod (06) capable of stirring the cells and the culture solution in the culture dish rotates in the inner tube (04).
6. The multifunctional intelligent cell culture dish of claim 5, wherein: the culture dish also comprises a groove plate (10) with a circular recess, the dish support (01) can be embedded in the circular recess and can slide on the groove plate (10) to press a dish cover (05), and a tension spring is connected between the press plate (11) and the groove plate (10).
7. The multifunctional intelligent cell culture dish of claim 6, wherein: the culture dish further comprises a sleeve (15) fixed in the middle of the circular recess and a rotating shaft (14) rotating in the sleeve (15), a rectangular block is formed on the rotating shaft (14), and a rectangular groove used for inserting the rectangular block is formed in the lower end of the rotating rod (06).
8. The multifunctional intelligent cell culture dish of claim 7, wherein: the lamp (13) is fixed on the sleeve (15), and the lamp (13) is provided with a power supply.
9. The multifunctional intelligent cell culture dish of claim 8, wherein: still include base (16), and swivel (17) of rotating on base (16), be fixed with friction ring (18) on swivel (17), base (16) have one through motor drive with friction ring (18) friction drive's friction pulley (19), frid (10) in a plurality of culture dishes all fix on swivel (17).
10. The multifunctional intelligent cell culture dish of claim 9, wherein: the device also comprises a flange (07) fixed on the base (16), and an elastic plate (12) made of elastic materials is fixed at the bottom end of the groove plate (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210601062.2A CN115029241A (en) | 2022-05-30 | 2022-05-30 | Multi-functional intelligent cell culture dish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210601062.2A CN115029241A (en) | 2022-05-30 | 2022-05-30 | Multi-functional intelligent cell culture dish |
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| Publication Number | Publication Date |
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| CN115029241A true CN115029241A (en) | 2022-09-09 |
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| CN202210601062.2A Pending CN115029241A (en) | 2022-05-30 | 2022-05-30 | Multi-functional intelligent cell culture dish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115926954A (en) * | 2023-02-09 | 2023-04-07 | 廖婷婷 | Cell culture dish capable of detecting temperature |
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2022
- 2022-05-30 CN CN202210601062.2A patent/CN115029241A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115926954A (en) * | 2023-02-09 | 2023-04-07 | 廖婷婷 | Cell culture dish capable of detecting temperature |
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