CN207362219U - A kind of suspending cell culture bottle - Google Patents
A kind of suspending cell culture bottle Download PDFInfo
- Publication number
- CN207362219U CN207362219U CN201721341004.1U CN201721341004U CN207362219U CN 207362219 U CN207362219 U CN 207362219U CN 201721341004 U CN201721341004 U CN 201721341004U CN 207362219 U CN207362219 U CN 207362219U
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- bottle
- bottleneck
- permeable membrane
- cell culture
- injection port
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Abstract
The utility model discloses a kind of suspending cell culture bottle, including hydrophobic permeable membrane and bottle, bottle one end connects injection port by bottleneck, injection port is connected with bottleneck thread, bottleneck is trapezoidal and one end side is provided with label, bottle is cuboid and is internally provided with upper strata culture layer and lower floor's culture layer, bottle upper and lower surface is provided with hydrophobic permeable membrane at the same time and side is provided with frosted area, the bottle other end is connected with sample tap by bottleneck, sample tap is connected with bottleneck thread, by creating newly-installed hydrophobic permeable membrane, increase the area of gas exchanges, solve the problems, such as that gas permeability is insufficient；By creating newly-installed injection port and sample tap, realize into separated function is sampled, add the accuracy of experiment；By creating newly-installed culture layer, double-deck setting can not only increase accommodates the upper limit to greatest extent in the case of blake bottle constancy of volume, but also can cultivate at the same time, solves the problems, such as that efficiency is low.
Description
Technical field
A kind of blake bottle is the utility model is related to, is specially a kind of suspending cell culture bottle.
Background technology
Cell growth does not depend on support surface, is grown in nutrient solution in suspended state, and this kind of cell is known as suspending thin
Born of the same parents, the culture that suspends refer to a kind of in the fluid nutrient medium for constantly being stirred or being shaken, the unicellular and small cell cluster of culture
Tissue culture system, be non-anchorage-dependent cell a kind of training method.
Existing suspending cell culture bottle in use, there are many problems, such as：There are asking for gas permeability deficiency
Topic, existing Tissue Culture Flask are all made of bottle and bottle cap, and bottle cap has gas permeability, for conveying gas for cell culture
Body, however, in the case where only bottle cap has gas permeability, it is smaller with the area of air exchange, limit the number of cell culture
Amount so that experimenter has to use many blake bottle cultures at the same time, wasting manpower and material resources, also increases the risk of pollution；
There are pollution risk it is big the problem of, present Tissue Culture Flask only has a bottle cap, result in sample introduction, sampling use it is same
A hole, be easy to cause pollution, influences the accuracy of experiment；There are efficiency it is low the problem of, cultivate a period of time after just need to expand
Culture, and expand culture and need replacing multiple Tissue Culture Flasks, multiple Tissue Culture Flasks do not allow manageability, lose time, efficiency
Lowly.
In conclusion there are the problem of gas permeability is insufficient, pollution risk is big low with efficiency.
The content of the invention
The technical problems to be solved in the utility model is to overcome existing blake bottle during the work time, gas permeability deficiency, dirt
Contaminate the defects of risk is big and efficiency is low, there is provided a kind of suspending cell culture bottle, so as to solve the above problems.
In order to solve the above-mentioned technical problem, the utility model provides following technical solution：
A kind of suspending cell culture bottle of the utility model, including hydrophobic permeable membrane and bottle, bottle one end are connected by bottleneck
Injection port is connect, injection port is connected with bottleneck thread, and bottleneck is trapezoidal and one end side is provided with label, and bottle is cuboid and interior
Portion is provided with upper strata culture layer and lower floor's culture layer, while bottle upper and lower surface is provided with hydrophobic permeable membrane and side is provided with mill
Sand area, the bottle other end are connected with sample tap by bottleneck, and sample tap is connected with bottleneck thread.
As a kind of optimal technical scheme of the utility model, bottle and bottleneck use high-quality, transparent non-renewable poly- second
Alkene, which is integrally formed, to be made, and the angle on the surface of bottleneck and bottle is obtuse angle, has high sterile level, is that non-adherent cell suspends
The ideal chose of culture.
As a kind of optimal technical scheme of the utility model, hydrophobic permeable membrane be square and be polytetrafluoroethylene film or
Polyvinylidene fluoride film, hydrophobic permeable membrane are embedded in bottle upper and lower surface and correspond to indentation, there, increase the area of gas exchanges, increase
The gas permeability of blake bottle, inlays and is arranged on indentation, there and can also reduce the cost and weight of blake bottle.
As a kind of optimal technical scheme of the utility model, injection port and the different color of sample tap surface smear and with
Bottle cap forms 1/3 corner, and closure inside is provided with ring type seal, and 1/3 corner fitted seal circle, realizes good in sealing performance
In the case of good, it is allowed to which the function of one-handed performance, facilitates the progress of experiment.
As a kind of optimal technical scheme of the utility model, upper strata culture layer is arranged on bottle medium position, lower floor's training
Foster layer is arranged on bottle body bottom, ensure that culture layer occupies identical space, facilitates the use of experiment.
The beneficial effect that the utility model is reached is：The utility model is increased by creating newly-installed hydrophobic permeable membrane
The areas of gas exchanges, adds the gas permeability of blake bottle, and the cell for increasing blake bottle is accommodated and reached the standard grade, and solves gas permeability
The problem of insufficient；By creating newly-installed injection port and sample tap, realize into sampling separated function, injection port periodically into
Sample, samples as desired by the sample tap of opposite side, not easy to pollute, adds the accuracy of experiment；It is newly-installed by creating
Culture layer, double-deck setting can not only increase accommodates the upper limit to greatest extent in the case of blake bottle constancy of volume, but also
It can at the same time cultivate, only need a blake bottle just to meet to test needs, avoid and replace multiple blake bottles, it is low to solve efficiency
The problem of.
Brief description of the drawings
Attached drawing is used to provide a further understanding of the present invention, and a part for constitution instruction, with this practicality
New embodiment is used to explain the utility model together, does not form the limitation to the utility model.
In the accompanying drawings：
Fig. 1 is the utility model overall structure diagram；
Fig. 2 is the utility model overlooking the structure diagram；
Fig. 3 is the utility model positive structure schematic；
Figure label：1st, injection port；2nd, bottleneck；3rd, label；4th, frosted area；5th, lower floor's culture layer；6th, bottle；7th, it is hydrophobic
Air film；8th, upper strata culture layer；9th, sample tap.
Embodiment
The preferred embodiment of the utility model is illustrated below in conjunction with attached drawing, it will be appreciated that described herein excellent
Select embodiment to be only used for describing and explaining the present invention, be not used to limit the utility model.
, it is necessary to explanation in the description of the utility model, the instruction such as term " vertical ", " on ", " under ", " level "
Orientation or position relationship are based on orientation shown in the drawings or position relationship, are for only for ease of description the utility model and simplification
Description, rather than the device or element of instruction or hint meaning must have specific orientation, with specific azimuth configuration and behaviour
Make, therefore it is not intended that limitation to the utility model.
In the description of the utility model, it is also necessary to which explanation, unless otherwise clearly defined and limited, term " are set
Put ", " installation ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected,
Or it is integrally connected；Can mechanically connect or be electrically connected；It can be directly connected, intermediary can also be passed through
It is indirectly connected, can is the connection inside two elements.For the ordinary skill in the art, can be according to specific
Situation understands concrete meaning of the above-mentioned term in the utility model.
Embodiment：As shown in Figs. 1-3, the utility model provides a kind of suspending cell culture bottle, including 7 He of hydrophobic permeable membrane
Bottle 6,6 one end of bottle connect injection port 1 by bottleneck 2, and injection port 1 is threadedly coupled with bottleneck 2, and bottleneck 2 is trapezoidal and one end
Side is provided with label 3, and bottle 6 is cuboid and is internally provided with upper strata culture layer 8 and lower floor's culture layer 5, while on bottle 6
Lower surface is provided with hydrophobic permeable membrane 7 and side is provided with frosted area 4, and 6 other end of bottle is connected with sample tap 9 by bottleneck 2,
Sample tap 9 is threadedly coupled with bottleneck 2.
Specifically, a kind of suspending cell culture bottle of the utility model, bottle 6 and bottleneck 2 are using high-quality, transparent non-renewable
Polyethylene, which is integrally formed, to be made, and bottleneck 2 and the angle on the surface of bottle 6 are obtuse angle, has high sterile level, is non-adherent thin
The ideal chose of born of the same parents' suspension culture；Hydrophobic permeable membrane 7 is square and is polytetrafluoroethylene film or polyvinylidene fluoride film, hydrophobic
Air film 7 is embedded in 6 upper and lower surface of bottle and corresponds to indentation, there, increases the area of gas exchanges, adds the gas permeability of blake bottle,
Inlay and be arranged on indentation, there and can also reduce the cost and weight of blake bottle；The face different with 9 surface smear of sample tap of injection port 1
Color and 1/3 corner is formed with bottle cap, closure inside is provided with ring type seal, and 1/3 corner fitted seal circle, realizes and sealing
In the case of functional, it is allowed to which the function of one-handed performance, facilitates the progress of experiment；Upper strata culture layer 8 is arranged in bottle 6
Portion position, lower floor's culture layer 5 are arranged on 6 bottom of bottle, ensure that culture layer occupies identical space, facilitate making for experiment
With.
Operation principle：During experiment, sample tap 9 is opened, sample and culture medium are put into by bottleneck 2, individually put as needed
Enter upper strata culture layer 8 and either lower floor's culture layer 5 or all put for two layers, the suspension for writing culture exactly on the label 3 of 2 side of bottleneck is thin
Born of the same parents, to identify, injection port 1 is different with 9 color of sample tap, and convenient to distinguish, when being put into culture, hydrophobic permeable membrane 7 being capable of big face
Long-pending exchanging gas, supplies the carbon dioxide and oxygen needed for cell growth, while not leak liquid, sometimes human hand it is inadequate when
Wait, singlehanded can pin the frosted area 4 of 6 both sides of bottle, turn on bottle cap sampling, 1/3 corner is opened and sealing ring can ensure
Sealing can be opened simply again at the same time, and the culture situation of suspension cell can be observed through bottle 6.
Finally it should be noted that：It these are only the preferred embodiment of the utility model, be not limited to this practicality
It is new, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art,
It can still modify the technical solution described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement.Where within the spirit and principles of the present invention, any modification, equivalent replacement, improvement and so on, should all include
Within the scope of protection of the utility model.
Claims (5)
1. a kind of suspending cell culture bottle, it is characterised in that including hydrophobic permeable membrane（7）And bottle（6）, bottle（6）One end leads to
Cross bottleneck（2）Connect injection port（1）, injection port（1）With bottleneck（2）It is threadedly coupled, bottleneck（2）Trapezoidal and one end side is set
There is label（3）, bottle（6）For cuboid and it is internally provided with upper strata culture layer（8）With lower floor's culture layer（5）, while bottle（6）
Upper and lower surface is provided with hydrophobic permeable membrane（7）And side is provided with frosted area（4）, bottle（6）The other end passes through bottleneck（2）Connection
There is sample tap（9）, sample tap（9）With bottleneck（2）It is threadedly coupled.
A kind of 2. suspending cell culture bottle according to claim 1, it is characterised in that bottle（6）And bottleneck（2）Using excellent
Matter, transparent non-renewable polyethylene are integrally formed and are made, and bottleneck（2）With bottle（6）The angle on surface be obtuse angle.
A kind of 3. suspending cell culture bottle according to claim 1, it is characterised in that hydrophobic permeable membrane（7）For square
And be polytetrafluoroethylene film or polyvinylidene fluoride film, hydrophobic permeable membrane（7）It is embedded in bottle（6）Upper and lower surface corresponds to indentation, there.
A kind of 4. suspending cell culture bottle according to claim 1, it is characterised in that injection port（1）And sample tap（9）Table
Face smears different colors and forms 1/3 corner with bottle cap, and closure inside is provided with ring type seal.
A kind of 5. suspending cell culture bottle according to claim 1, it is characterised in that upper strata culture layer（8）It is arranged on bottle
Body（6）Medium position, lower floor's culture layer（5）It is arranged on bottle（6）Bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721341004.1U CN207362219U (en) | 2017-10-18 | 2017-10-18 | A kind of suspending cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721341004.1U CN207362219U (en) | 2017-10-18 | 2017-10-18 | A kind of suspending cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207362219U true CN207362219U (en) | 2018-05-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721341004.1U Active CN207362219U (en) | 2017-10-18 | 2017-10-18 | A kind of suspending cell culture bottle |
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| CN (1) | CN207362219U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504574A (en) * | 2018-07-05 | 2018-09-07 | 广州瑞铂茵健康管理咨询有限公司 | Culture bottle |
| CN109022281A (en) * | 2018-08-16 | 2018-12-18 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel cell culture vessel |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN113061521A (en) * | 2021-04-06 | 2021-07-02 | 英诺维尔智能科技（苏州）有限公司 | High-effect cell culture tube |
-
2017
- 2017-10-18 CN CN201721341004.1U patent/CN207362219U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504574A (en) * | 2018-07-05 | 2018-09-07 | 广州瑞铂茵健康管理咨询有限公司 | Culture bottle |
| CN109022281A (en) * | 2018-08-16 | 2018-12-18 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel cell culture vessel |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN113061521A (en) * | 2021-04-06 | 2021-07-02 | 英诺维尔智能科技（苏州）有限公司 | High-effect cell culture tube |
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