CN109022281A - A kind of novel cell culture vessel - Google Patents
A kind of novel cell culture vessel Download PDFInfo
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- CN109022281A CN109022281A CN201810933072.XA CN201810933072A CN109022281A CN 109022281 A CN109022281 A CN 109022281A CN 201810933072 A CN201810933072 A CN 201810933072A CN 109022281 A CN109022281 A CN 109022281A
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- rigid structure
- vessel
- cell culture
- culture vessel
- culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of novel cell culture vessels, including rigid structure and membrane structure, the rigid structure is internally provided with cavity, and cavity upper and lower surfaces are provided with membrane structure, and rigid structure front end is connected with bottleneck, bottleneck is connected with bottle cap, the bottleneck is connected with cavity, and the rigid structure lower end is connected with vessel shoulder, and the rigid structure side is provided with notch, the rigid structure rear end is connected with suspension column, and suspension column is connected with culture dish bottom；The cell culture vessel of the utility model, so that floated cell reaches the same effect in culture bag in the new design vessel that bottle bag combines；Automatic machinery people can accurately capture culture vessel simultaneously；And the V-shape of culture vessel bottom after the completion of being conducive to cell growth cycle, facilitates and liquid is all taken out from culture vessel.
Description
Technical field
The present invention relates to culture vessel equipment technical field, specially a kind of novel cell culture vessel.
Background technique
Cell culture container culture bottle or culture bag general at present.Both culture vessels, in hand
Work operation culture cell in history, once plays the role of critical；And currently, being still popular cell culture
Mode.
But existing culture bag and culture bottle, it is unsuitable for Highgrade integration, totally-enclosed, high-throughput cell culture rule
Modelling production.
Existing cell culture container is closed with matching for automatic operation, and there are following main problems.
1, the placement of culture bag is grabbed with catching；
2, the gas permeability and hydrophobe of culture bottle；
3, the mechanism of uncapping of culture bag and culture bottle can not be realized in the environment of automation.
Summary of the invention
The purpose of the present invention is to provide a kind of novel cell culture vessels, to solve cell culture bags in semi-automation
With the storing and access issues in automated production；Solve the problems, such as uncapping in cell cultivation process；Solve the receipts of cell culture
Beneficial problem.
To achieve the above object, the invention provides the following technical scheme: a kind of novel cell culture vessel, including rigidity
Structure and membrane structure, the rigid structure are internally provided with cavity, and cavity upper and lower surfaces are provided with membrane structure, just
Property structure front end is connected with bottleneck, and bottleneck is connected with bottle cap, and the bottleneck is connected with cavity, and the rigid structure lower end is connected with
Vessel shoulder, the rigid structure side are provided with notch, and the rigid structure rear end is connected with suspension column, and suspension column is connected with culture
Ware bottom.
Preferably, the rigid structure is made of PS or equivalent material.
Preferably, the membrane structure is composite membrane, is made of PE/EVA/PE or equivalent material.
Preferably, the junction of the rigid structure of the culture dish and membrane structure uses ultrasonic welding and HF hot melt work
Skill processing.
Preferably, the bottom of the culture dish is rendered as V-shaped.
Preferably, two pin holes are arranged in the vessel shoulder.
Compared with prior art, beneficial effects of the present invention are as follows:
1. the cell culture vessel of the utility model, floated cell reaches and is cultivating in the new design vessel that bottle bag combines
Same effect in bag；
2. the cell culture vessel of the utility model realizes positioning by setting pin hole and suspension column, automatic machinery people being capable of essence
Really capture culture vessel；
3. the cell culture vessel of the utility model, the V-shape of culture vessel bottom, after the completion of being conducive to cell growth cycle,
Facilitate and liquid is all taken out from culture vessel.
Detailed description of the invention
Fig. 1 is 3D schematic diagram one of the invention；
Fig. 2 is 3D schematic diagram two of the invention；
Fig. 3 is 3D schematic diagram three of the invention；
Fig. 4 is 3D schematic diagram four of the invention；
Fig. 5 is 3D schematic diagram five of the invention；
Fig. 6 is 3D schematic diagram six of the invention；
Fig. 7 is 3D schematic diagram seven of the invention.
In figure: 1, rigid structure；2, membrane structure；3, cavity；4, bottleneck；5, bottle cap；6, culture dish bottom；7, suspension column；
8, vessel shoulder；81, pin hole；9, notch.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-7, a kind of novel cell culture vessel, including rigid structure 1 and membrane structure 2, rigid structure 1
Using PS or equivalent material, framework part is firm, so that cell culture is gone on smoothly；Rigid structure 1 is internally provided with cavity 3,
3 upper and lower surfaces of cavity are provided with membrane structure 2, and membrane structure 2 is composite membrane, using PE/EVA/PE or equivalent material,
As the basic material of cell culture and ventilative exchange, major function is that oxygen (gas) is allowed to enter culture dish, is hindered simultaneously
It cuts off the water supply and enters culture dish；The rigid structure 1 of culture dish and the junction of membrane structure 2 use ultrasonic welding and HF hot melt technique
Processing guarantees gas No leakage；1 front end of rigid structure is connected with bottleneck 4, and bottleneck 4 is connected with bottle cap 5, and bottle cap 5 is solid cover,
Center does not need hollow out, absolute guarantee leakproofness and no leakage, and bottle cap 5 is easily opened and recloses；Bottleneck 4 is connected with cavity
3,3 lower end of cavity is connected with vessel shoulder 8, and 8 shape of vessel shoulder is divided into triangle and tubular, two pins of the setting of vessel shoulder 8
The positioning when function in hole 81, pin hole 81 lays flat for vessel；1 side of rigid structure is provided with notch 9, and 1 rear end of rigid structure connects
It is connected to suspension column 7, the function of suspension column 7 is that vessel are used as positioning when standing；Suspension column 7 is connected with culture dish bottom 6, the bottom of culture dish
It is rendered as V-shaped, is conducive to for liquid being all sucked out；Big penetrating film is divided into several small penetrating films above Fig. 6 bottle, is realized
The refinement of scheme；The combination of several small penetrating films is substituted above Fig. 7 bottle with big penetrating film.
In summary: the cell culture vessel of the utility model, so that floated cell is in the new design vessel that bottle bag combines,
Reach the same effect in culture bag；Positioning is realized by setting pin hole 7 and suspension column 81 simultaneously, automatic machinery people being capable of essence
Really capture culture vessel；And the V-shaped structure in culture vessel bottom 6, after the completion of being conducive to cell growth cycle, facilitates liquid
Body all takes out from culture vessel.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of novel cell culture vessel, including rigid structure (1) and membrane structure (2), it is characterised in that: the rigidity
Structure (1) is internally provided with cavity (3), and cavity (3) upper and lower surfaces are provided with membrane structure (2), before rigid structure (1)
End is connected with bottleneck (4), and bottleneck (4) is connected with bottle cap (5), and the bottleneck (4) is connected with cavity (3), the rigid structure (1)
Lower end is connected with vessel shoulder (8), and rigid structure (1) side is provided with notch (9), and rigid structure (3) rear end connects
It is connected to suspension column (7), suspension column (7) is connected with culture dish bottom (6).
2. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the rigid structure (1) is adopted
It is made of PS or equivalent material.
3. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the membrane structure (2) is
Composite membrane is made of PE/EVA/PE or equivalent material.
4. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the rigid structure (1) and
The junction of membrane structure (2) uses ultrasonic welding and HF hot melt process.
5. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the culture dish bottom (5)
It is rendered as V-shaped.
6. the novel cell culture vessel of one kind according to claim 1, it is characterised in that: the vessel shoulder (8) sets
Set two pin holes (81).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933072.XA CN109022281A (en) | 2018-08-16 | 2018-08-16 | A kind of novel cell culture vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933072.XA CN109022281A (en) | 2018-08-16 | 2018-08-16 | A kind of novel cell culture vessel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109022281A true CN109022281A (en) | 2018-12-18 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810933072.XA Pending CN109022281A (en) | 2018-08-16 | 2018-08-16 | A kind of novel cell culture vessel |
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| Country | Link |
|---|---|
| CN (1) | CN109022281A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4249581A3 (en) * | 2019-02-25 | 2023-12-06 | University of Maryland, Baltimore County | Adjustable fermentation and cell culture flasks |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0700990A2 (en) * | 1994-09-12 | 1996-03-13 | Becton, Dickinson and Company | Roller Bottle for trans-membrane co-culture of cells and method for its use |
| US20050148068A1 (en) * | 2003-12-31 | 2005-07-07 | Lacey William J. | Cell cultivating flask |
| CN101787345A (en) * | 2010-03-10 | 2010-07-28 | 西安交通大学 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
| JP2016067279A (en) * | 2014-09-30 | 2016-05-09 | 新日鉄住金化学株式会社 | Polyimide film for organism growth and container for organism growth |
| JP5919776B2 (en) * | 2011-12-02 | 2016-05-18 | 大日本印刷株式会社 | Method for producing cell culture vessel |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN107109341A (en) * | 2014-10-29 | 2017-08-29 | 康宁股份有限公司 | Method and apparatus for generating and cultivating 3D cell aggregations |
| CN207362219U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspending cell culture bottle |
-
2018
- 2018-08-16 CN CN201810933072.XA patent/CN109022281A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0700990A2 (en) * | 1994-09-12 | 1996-03-13 | Becton, Dickinson and Company | Roller Bottle for trans-membrane co-culture of cells and method for its use |
| US20050148068A1 (en) * | 2003-12-31 | 2005-07-07 | Lacey William J. | Cell cultivating flask |
| CN101787345A (en) * | 2010-03-10 | 2010-07-28 | 西安交通大学 | Device for collecting and separating body fluid sample used for cell examination and method therefor |
| JP5919776B2 (en) * | 2011-12-02 | 2016-05-18 | 大日本印刷株式会社 | Method for producing cell culture vessel |
| JP2016067279A (en) * | 2014-09-30 | 2016-05-09 | 新日鉄住金化学株式会社 | Polyimide film for organism growth and container for organism growth |
| CN107109341A (en) * | 2014-10-29 | 2017-08-29 | 康宁股份有限公司 | Method and apparatus for generating and cultivating 3D cell aggregations |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN207362219U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspending cell culture bottle |
Non-Patent Citations (1)
| Title |
|---|
| 化学工业部人事教育司: "《化工防腐塑料》", 30 November 1997, 化学工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4249581A3 (en) * | 2019-02-25 | 2023-12-06 | University of Maryland, Baltimore County | Adjustable fermentation and cell culture flasks |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181218 |