CN218879937U - Cell culture dish with humidity maintaining function - Google Patents
Cell culture dish with humidity maintaining function Download PDFInfo
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- CN218879937U CN218879937U CN202320530383.8U CN202320530383U CN218879937U CN 218879937 U CN218879937 U CN 218879937U CN 202320530383 U CN202320530383 U CN 202320530383U CN 218879937 U CN218879937 U CN 218879937U
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- culture dish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
A cell culture dish with humidity maintaining function relates to the technical field of cell culture and is used for maintaining the humidity of the environment where cells are located in the process of cell culture. Including culture dish main part and ware lid, culture dish main part inboard has the culture table, form the basin between culture table and the culture dish main part inner wall, a plurality of cultivation holes have on the culture table, the downthehole cell of waiting to cultivate that is used for placing of cultivation, the top at the culture dish main part is detained to the ware lid. The utility model discloses set up inside and outside independent setting's culture hole and basin, at cell culture's in-process, the humidity between culture dish main part and the ware lid is kept in the evaporation of water for the cultivation in the basin, and then provides moist environment for cell culture.
Description
Technical Field
The utility model belongs to the technical field of the cell culture technique and specifically relates to a cell culture dish with humidity retention effect.
Background
The cell culture dish is a laboratory vessel for cell culture, and during the cell culture process, various physicochemical factors in the environment can influence the development of cells and even cause abnormal cell development, and the physicochemical factors comprise temperature, humidity, oxygen concentration and the like. Wherein, humidity indirectly affects the ovum and embryo cells, and mainly indirectly affects the development of the ovum and embryo by affecting the concentration and osmotic pressure of various components in the culture solution.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture dish with humidity retention for keep the humidity of the environment that the cell was located at cell culture's in-process.
The utility model provides a technical scheme that its technical problem adopted is: cell culture dish with humidity retention effect, including culture dish main part and ware lid, culture dish main part inboard has the culture table, form the basin between culture table and the culture dish main part inner wall, the culture table is last to have a plurality of cultivation holes, the downthehole cell of waiting to cultivate that is used for placing of cultivation, the top at the culture dish main part is detained to the ware lid.
Further, the upper surface of the culture platform is internally provided with a groove, the culture hole is positioned below the groove, and the top of the culture hole is communicated with the bottom of the groove.
Further, the cultivation platform is of a circular structure, and the water tank is of an annular structure.
Furthermore, the culture dish main body is of a conical structure with a large upper end and a small lower end, and the lower part of the dish cover is inserted into the upper end of the culture dish main body.
Furthermore, the culture dish main part is a conical structure with a large upper end and a small lower end, and the upper part of the culture dish main part extends into the inner side of the dish cover.
Furthermore, the upper surface of the culture platform is also provided with a culture solution sucking hole.
Further, the aspirated culture medium wells communicate with part or all of the culture wells.
Further, the bottom of culture dish main part inner chamber has the collar, the culture table bottom has the mounting groove, the collar outer wall has the external screw thread, the mounting groove inner wall has the internal thread, the collar realizes the fixed connection of culture table and culture dish main part with mounting groove threaded connection.
Further, the bottom of the culture dish main body is provided with a base.
Further, the culture wells are arranged in a matrix form.
The beneficial effects of the utility model are that: the utility model discloses set up inside and outside independent setting's culture hole and basin, at cell culture's in-process, the humidity between culture dish main part and the ware lid is kept in the evaporation of water for the cultivation in the basin, and then provides moist environment for cell culture, and then realizes keeping cell culture's humidity.
Drawings
Fig. 1 is a three-dimensional view of a first embodiment of the present invention;
fig. 2 is a front view of a first embodiment of the present invention;
FIG. 3 isbase:Sub>A cross-sectional view A-A of FIG. 2;
FIG. 4 is a three-dimensional view of a main body of a culture dish according to a first embodiment of the present invention;
FIG. 5 is a second three-dimensional view of the main body of the culture dish according to the first embodiment of the present invention;
FIG. 6 is a top view of a main body of a culture dish according to a first embodiment of the present invention;
FIG. 7 is a cross-sectional view taken along line B-B of FIG. 6;
FIG. 8 is a cross-sectional view taken at C-C of FIG. 6;
fig. 9 is a three-dimensional view of a second embodiment of the present invention;
fig. 10 is a front view of a second embodiment of the present invention;
FIG. 11 is a cross-sectional view of a main body of a culture dish according to a third embodiment of the present invention;
in the figure: the culture dish comprises a culture dish main body 1, a base 11, a water tank 12, a mounting ring 13, a dish cover 2, a handle 21, a culture platform 3, a groove 31, a culture hole 32, a culture liquid suction hole 33 and a communication channel 34.
Detailed Description
As shown in fig. 1 to 11, the present invention includes a culture dish main body 1 and a dish cover 2, and the present invention will be described in detail with reference to the accompanying drawings.
The first embodiment is as follows:
as shown in fig. 1 to 8, the cell culture dish with humidity maintaining function includes a culture dish main body 1 and a dish cover 2, the culture dish main body 1 is a conical structure with a large upper end and a small lower end, and the top of the culture dish main body 1 is open. As shown in fig. 2 and 3, the dish cover 2 is also of a conical structure, the upper end of the dish cover 2 is large, the lower end is small, and the upper end of the dish cover 2 is provided with a handle 21. The lower end of the dish cover 2 is inserted into the upper end of the culture dish main body 1, and when the lower part of the dish cover 2 extends into the culture dish main body 1, the lower part of the dish cover 2 is in surface contact with the culture dish main body 1, and the contact surface is an annular conical surface.
The culture dish main part 1 is provided with a culture platform 3 on the inner side, the culture platform 3 is of a circular structure, a water tank 12 is formed between the culture platform 3 and the inner wall of the culture dish main part 1, and the water tank 12 is of an annular structure. The culture table 3 has a plurality of culture wells 32, and the culture wells 32 are independent of the water tank 12. The culture stage 3 is provided to maintain the communication between the culture wells 32 and to isolate the water tank 12 from the culture wells 32. Be used for placing in the culture hole 32 and wait to cultivate the cell, the top at culture dish main part 1 is detained to dish lid 2, realizes the closure to culture dish main part 1 inner chamber for in the culture hole 32 cell is in the confined environment. As shown in FIG. 7, the culture platform 3 has a circular groove 31 in the upper surface thereof, and the groove 31 is arranged so that the height of the culture well 32 is lower than the height of the culture platform 3, that is, the top of the culture well 32 is lower than the upper surface of the culture platform 3, and the height of the culture platform 3 is lower than the height of the culture dish body 1.
The culture well 32 is located below the groove 31, and the top of the culture well 32 communicates with the bottom of the groove 31. The culture wells 32 are arranged in a matrix on the culture table 3, and the number of the culture wells 32 may be as many as necessary, and as shown in FIG. 4, FIG. 5, and FIG. 6, the number of the culture wells 32 is 25 in a 5X 5 arrangement. The culture holes 32 are circular holes, and the culture holes 32 penetrate the upper and lower surfaces of the culture table 3.
In order to facilitate the placing of the culture dish main body 1, as shown in fig. 3, the bottom of the culture dish main body 1 is provided with a base 11, the base 11 is of a circular ring structure, and the base 11 and the culture dish main body 1 are integrally manufactured and formed.
In order to facilitate the aspiration of the culture medium in the culture well 32, as shown in FIG. 6, an aspirated culture medium well 33 is further provided in the upper surface of the culture table 3. The culture solution suction hole 33 is communicated with part or all of the culture holes 32, and specifically comprises: as shown in FIG. 8, a communication channel 34 is provided between the aspirated culture medium hole 33 and the culture hole 32, one end of the communication channel 34 communicates with the aspirated culture medium hole 33, and the other end of the communication channel 34 communicates with the culture hole 32. When the culture medium in the culture medium suction hole 33 is sucked, the volume of the culture medium on the culture table 3 is reduced, and at this time, the culture medium in the culture medium suction hole 33 is reduced, and the culture medium in the culture hole 32 flows into the culture medium suction hole 33, thereby sucking the culture medium.
The following describes the use of the present invention:
(1) Placing a culture solution in the culture well 32;
(2) The water tank 12 is filled with culture water, and the dish cover 2 is closed.
At cell culture's in-process, enclose into confined environment between the setting of ware lid 2 and the culture dish main part 1, and the steam gathering after the evaporation of culture water in the basin 12 is between ware lid 2 and culture dish main part 1, and then keeps the humidity between ware lid 2 and the culture dish main part 1, provides suitable humidity for cell culture.
Example two:
as shown in fig. 9 to 10, the second embodiment is different from the first embodiment in that the upper portion of the culture dish main body 1 extends into the dish cover 2, so as to seal the top of the culture dish main body 1, and the second embodiment of the present invention is described in detail below. Cell culture dish with humidity retention includes culture dish main part 1 and ware lid 2, and culture dish main part 1 is the big, the little toper structure of lower extreme in upper end, and the top of culture dish main part 1 is uncovered. The dish cover 2 is also in a conical structure, the upper end of the dish cover 2 is large, the lower end of the dish cover 2 is small, and the upper end of the dish cover 2 is provided with a handle 21. As shown in fig. 9 and 10, the lower end of the dish cover 2 is fitted over the outer side of the upper end of the culture dish main body 1. The lower part of the dish cover 2 is in surface contact with the culture dish main body 1, and the contact surface is an annular conical surface, so that the inner cavity of the culture dish main body 1 is sealed.
The culture dish main part 1 inboard has culture table 3, and culture table 3 is circular structure, forms basin 12 between culture table 3 and the culture dish main part 1 inner wall, and basin 12 is the ring structure. The culture table 3 has a plurality of culture wells 32, and the culture wells 32 are independent of the water tank 12. The culture stage 3 is provided to maintain the communication between the culture wells 32 and to isolate the water tank 12 from the culture wells 32. The culture holes 32 are used for placing cells to be cultured, the upper surface of the culture platform 3 is provided with a circular groove 31, the groove 31 is arranged, so that the height of the culture holes 32 is lower than that of the culture platform 3, namely, the top of the culture holes 32 is lower than the upper surface of the culture platform 3, and the height of the culture platform 3 is lower than that of the culture dish main body 1.
The culture well 32 is located below the groove 31, and the top of the culture well 32 communicates with the bottom of the groove 31. The culture wells 32 are arranged in a matrix on the culture table 3, and the number of the culture wells 32 may be as many or as few as necessary. The culture holes 32 are circular holes, and the culture holes 32 penetrate the upper and lower surfaces of the culture table 3. In order to facilitate the placing of the culture dish main body 1, the bottom of the culture dish main body 1 is provided with a circular ring-shaped base 11, and the base 11 and the culture dish main body 1 are integrally manufactured and formed.
In order to facilitate the aspiration of the culture medium in the culture well 32, a culture medium aspiration hole 33 is further provided in the upper surface of the culture platform 3. The culture solution suction hole 33 is communicated with part or all of the culture holes 32, and specifically comprises: a communication channel 34 is provided between the aspirated culture medium hole 33 and the culture hole 32, one end of the communication channel 34 communicates with the aspirated culture medium hole 33, and the other end of the communication channel 34 communicates with the culture hole 32. When the culture medium in the culture medium suction hole 33 is sucked, the volume of the culture medium on the culture table 3 is reduced, and at this time, the culture medium in the culture medium suction hole 33 is reduced, and the culture medium in the culture hole 32 flows into the culture medium suction hole 33, thereby sucking the culture medium.
Example three:
as shown in FIG. 11, the third embodiment is different from the first embodiment in the way of assembling the culture platform 3 and the culture dish main body 1. The method specifically comprises the following steps: as shown in fig. 11, the bottom of the inner cavity of the culture dish main body 1 is provided with an annular mounting ring 13, the bottom of the culture table 3 is provided with an annular mounting groove, the outer wall of the mounting ring 13 is provided with an external thread, the inner wall of the mounting groove is provided with an internal thread, and the mounting ring 13 extends into the mounting groove and is in threaded connection with the mounting groove, so that the fixed connection between the culture table 3 and the culture dish main body 1 is realized. The threaded connection mode between the culture platform 3 and the culture dish main body 1 can also facilitate the disassembly between the culture platform 3 and the culture dish main body 1. To ensure the sealing property between the culture platform 3 and the culture dish main body 1, a seal is provided between the culture platform 3 and the culture dish main body 1 to prevent the mixing of the culture water in the water tank 12 and the culture solution in the culture hole 32.
The utility model discloses set up inside and outside independent setting's culture hole and basin, at cell culture's in-process, the humidity between culture dish main part and the ware lid is kept in the evaporation of water for the cultivation in the basin, and then provides moist environment for cell culture.
Claims (10)
1. Cell culture dish with humidity retaining effect, its characterized in that, including culture dish main part and ware lid, culture dish main part inboard has the culture table, form the basin between culture table and the culture dish main part inner wall, the culture bench has a plurality of cultivation holes, be used for placing in the cultivation hole and wait to cultivate the cell, the top at the culture dish main part is detained to the ware lid.
2. A cell culture dish with humidity maintaining function according to claim 1, wherein the culture platform has a groove in the upper surface, the culture well is located below the groove, and the top of the culture well is communicated with the bottom of the groove.
3. A cell culture dish having a humidity maintaining effect according to claim 1, wherein the culture platform has a circular structure and the water tank has an annular structure.
4. The cell culture dish with the humidity maintaining effect according to claim 1, wherein the culture dish main body is of a conical structure with a large upper end and a small lower end, and the lower part of the dish cover is inserted into the upper end of the culture dish main body.
5. A cell culture dish with a humidity maintaining effect according to claim 1, wherein the culture dish main body is of a conical structure with a large upper end and a small lower end, and the upper part of the culture dish main body extends into the inner side of the dish cover.
6. A cell culture dish with humidity maintaining function according to claim 1, wherein the culture platform further has culture solution sucking holes in the upper surface thereof.
7. A cell culture dish with humidity maintaining function according to claim 6, wherein the culture solution sucking hole is communicated with part or all of the culture holes.
8. A cell culture dish with humidity maintaining effect according to claim 1, wherein the bottom of the inner cavity of the culture dish main body is provided with a mounting ring, the bottom of the culture platform is provided with a mounting groove, the outer wall of the mounting ring is provided with an external thread, the inner wall of the mounting groove is provided with an internal thread, and the mounting ring is in threaded connection with the mounting groove to realize the fixed connection of the culture platform and the culture dish main body.
9. A cell culture dish with humidity maintaining effect according to claim 1, wherein the bottom of the culture dish body has a base.
10. A cell culture dish having a humidity maintaining effect according to claim 1, wherein the culture wells are arranged in a matrix form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320530383.8U CN218879937U (en) | 2023-03-17 | 2023-03-17 | Cell culture dish with humidity maintaining function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320530383.8U CN218879937U (en) | 2023-03-17 | 2023-03-17 | Cell culture dish with humidity maintaining function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218879937U true CN218879937U (en) | 2023-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320530383.8U Active CN218879937U (en) | 2023-03-17 | 2023-03-17 | Cell culture dish with humidity maintaining function |
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| Country | Link |
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| CN (1) | CN218879937U (en) |
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2023
- 2023-03-17 CN CN202320530383.8U patent/CN218879937U/en active Active
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