CN220224189U - Organ-like culture device - Google Patents
Organ-like culture device Download PDFInfo
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- CN220224189U CN220224189U CN202320137986.1U CN202320137986U CN220224189U CN 220224189 U CN220224189 U CN 220224189U CN 202320137986 U CN202320137986 U CN 202320137986U CN 220224189 U CN220224189 U CN 220224189U
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- 210000002220 organoid Anatomy 0.000 claims abstract description 47
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 238000002347 injection Methods 0.000 claims abstract description 32
- 239000007924 injection Substances 0.000 claims abstract description 32
- 239000012528 membrane Substances 0.000 claims abstract description 18
- 238000001914 filtration Methods 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 230000000149 penetrating effect Effects 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 229920005669 high impact polystyrene Polymers 0.000 claims description 3
- 239000004797 high-impact polystyrene Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 17
- 238000011109 contamination Methods 0.000 abstract description 6
- 238000007789 sealing Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 239000007789 gas Substances 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 6
- 210000000056 organ Anatomy 0.000 description 5
- 238000012258 culturing Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an organoid culture device, and belongs to the technical field of medical appliances. The device comprises a culture dish body, wherein a plurality of culture cavities with the bottoms being sealed are arranged on the culture dish body; the culture dish cover is provided with a hollow liquid injection channel penetrating through the cover body of the culture dish cover; the liquid injection channel downwards extends out of the cover body of the culture dish cover to form a diversion part, the bottom of the diversion part is an inclined plane with an elliptical opening, and the bottom end of the inclined plane is close to the side wall of the culture cavity; the liquid injection channel is internally provided with a filtering membrane which is a bacterial filtering membrane. The organoid culture device provided by the application can effectively avoid the problem of bacterial contamination caused by frequently opening and closing the culture dish while ensuring the mildness and adding the reagent.
Description
Technical Field
The utility model relates to the technical field of medical equipment, in particular to an organ-like culture device.
Background
Organoids belong to three-dimensional (3D) cell cultures, which contain some key properties that represent organs. Such in vitro culture systems comprise a self-renewing stem cell population that can differentiate into a plurality of organ-specific cell types, possess similar spatial organization to the corresponding organ and are capable of reproducing part of the function of the corresponding organ, thereby providing a highly physiologically relevant system. Therefore, the organoid is often utilized to reproduce the physiological functions of a certain type of organ or is used as an in vitro model of various diseases at present, and has wide application prospect in a plurality of fields such as stem cells and development, regenerative medicine, disease research, drug development, tumor treatment and the like.
The early stages of 3D organoids are currently done in multi-well plates (e.g., 96-well or 384-well plates). In the process of adding the new reagent, the new reagent is directly dripped above in the existing method, so that the reagent directly contacts the organoid without dilution and has a certain stimulation effect on the organoid. In addition, operators need to frequently open and close the multi-hole culture plate cover to add liquid, and the problems of bacteria infection, organ-like bacteria infection, culture failure and the like are extremely easy to occur due to improper sterilization or improper operation in the process. Therefore, the current organoid culture experiments have high requirements for operators and environmental requirements.
Thus, there is a need for an organoid culture device that is gentle to add reagents, simple to operate, and not prone to contamination.
Disclosure of Invention
The utility model aims to provide an organoid culture device which can be mildly added with a reagent, is simple and convenient to operate and is not easy to stain bacteria, and solves the problem of bacteria staining caused by frequently opening and closing a culture dish in the organoid culture process.
In order to solve the above problems, in one aspect, the present utility model provides an organoid culture device comprising:
the culture dish comprises a culture dish body, wherein a plurality of culture cavities with the bottoms being sealed are arranged on the culture dish body;
the culture dish cover is provided with a hollow liquid injection channel penetrating through the cover body of the culture dish cover; the liquid injection channel downwards extends out of the cover body of the culture dish cover to form a flow guide part, the bottom of the flow guide part is an inclined plane with an elliptical opening, and the bottom end of the inclined plane is close to the side wall of the culture cavity; a filtering membrane is arranged in the liquid injection channel, and the filtering membrane is a bacterial filtering membrane.
After entering the liquid injection channel, the liquid flows along the flow guide part and enters the culture cavity. The bottom of the flow guiding part is provided with an elliptical inclined plane with an opening, so that liquid confluence can be better realized, and liquid splashing is avoided as much as possible.
In addition, a bacterial filter membrane is fixed in the liquid injection channel. On the one hand, the liquid injection channel can be used as a ventilation duct to realize gas exchange inside and outside the culture dish. In addition, in the gas exchange process, bacteria in the external environment are trapped by the bacteria filtering membrane, so that the sterile environment in the culture dish is ensured. On the other hand, the operator can realize the liquid feeding through the liquid injection channel, and can supplement the culture solution or inject specific reagents without uncovering, thereby effectively avoiding the problem of bacteria contamination caused by frequent opening and closing of the culture dish or deep penetration of the pipetting instrument into the culture chamber.
In a preferred embodiment, the radius of the filling channel is 0.1-0.2cm.
Further, the culture cavity comprises a first culture chamber and a second culture chamber which are sequentially connected from top to bottom, the horizontal cross-sectional area of the second culture chamber is smaller than that of the first culture chamber, and a step surface is formed at the bottom of the first culture chamber.
Preferably, the bottom of the flow guiding part is located above the step surface.
Wherein, the organoid culture is cultivated in the second culture room, because the aperture of the second culture room is slightly smaller than that of a normal culture dish, the nutrient solution needed by the cultivation is relatively less, and the cultivation material is saved more. And, in the actual culturing process, the applicant found that the organoid culture cultured in the second culture chamber had a shorter culturing period and better reproducibility than that of the organoid culture cultured in the ordinary culture dish.
In a preferred embodiment, the guiding part bottom is located step face top, and guiding part one side outer wall is hugged closely first cultivate the room lateral wall for liquid flows into the step face along first cultivate room lateral wall, and then along the step face entering second cultivate in, compare liquid direct drip in organoid culture top, the gentle of this application mode is less to organoid culture influence.
Further, the first culture chamber and the second culture chamber are cylindrical cavities, the radius of the bottom surface of the first culture chamber is 1-2cm, and the radius of the bottom surface of the second culture chamber is 0.3-0.9cm.
Preferably, the radius of the bottom surface of the first culture chamber is 1.74cm, and the radius of the bottom surface of the second culture chamber is 0.88cm.
Further, the liquid injection channel extends upwards to form a sealing part.
Further, the organoid culture device further comprises a sealing cover, and the sealing cover is nested and fixed with the sealing part.
Wherein, the person skilled in the art can choose the sealing cover to be taken down or covered up according to the actual situation. If the anaerobic culture stage is performed, the sealing cover can be selectively covered, and if the oxygen demand is large or exchange with external gas is needed, the sealing cover can be selectively removed.
The organoid culture device in the application can be covered with the sealing cover in a non-use state, so that the internal sterile environment is ensured.
Furthermore, the inner side of the culture dish cover is provided with a sealing gasket, and the sealing gasket is made of rubber.
The sealed pad that is equipped with in this application in culture dish lid inboard, so set up can block the gas exchange between the different culture chambers on the same culture dish, prevent that one of them from cultivateing the phenomenon in a plurality of culture chambers around the infection after the fungus is infected to the chamber.
Further, the culture dish cover and the culture dish body are made of one of glass, acrylic and ABS, HIPS, PP, PE.
In a preferred embodiment, the cover and the body are made of transparent glass, and the transparent plate facilitates the operator to observe the condition of the organoid culture through the plate and determine the growth stage of the organoid culture.
The application can bring the following beneficial effects:
1. the organoid culture device provided by the application can realize gentle injection of the liquid reagent by arranging the liquid injection channel, so that the problem of organoid damage caused by overlarge environmental difference in the new reagent adding process is avoided;
2. be equipped with bacterial filtration membrane in annotating the liquid passageway in this application, can directly realize gaseous exchange and aseptic injection liquid reagent through annotating the liquid passageway, effectively avoided frequently opening and shutting culture dish and pipetting instrument to go deep into the bacterial contamination problem that cultivates the cavity and bring.
3. The organoid culture device provided by the application is especially suitable for the condition that culture solution and inducer are required to be supplemented for multiple times in the organoid induction culture stage, and the reagent can be supplemented without opening and closing a culture dish, so that the organoid culture device is convenient and quick, and labor is greatly saved.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute an undue limitation to the application. In the drawings:
FIG. 1 shows a schematic diagram of an exemplary embodiment of an organoid culture device of the utility model;
FIG. 2 illustrates a cross-sectional view of an exemplary embodiment of an organoid culture device of the utility model in use;
FIG. 3 illustrates a cross-sectional view of an exemplary embodiment of an organoid culture device of the utility model in a non-use state.
In the figure: 1. the culture dish comprises a culture dish body, 2, a culture dish cover, 3, a liquid injection channel, 4, a flow guiding part, 5, a filtering membrane, 6, a culture cavity, 7, a first culture chamber, 8, a second culture chamber, 9, a step surface, 10, a sealing pad, 11, a sealing part, 12, a sealing cover, 13, an organoid culture, 14, a syringe, 15 and a pipette gun head.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present application will be described in detail below with reference to the following detailed description and the accompanying drawings.
As shown in the drawings, the following detailed description is given by way of example in connection with the accompanying drawings of the specification in order to more clearly explain the overall concepts of the present application.
In addition, in the description of the present application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "axial," "radial," "circumferential," etc. indicate or refer to an azimuth or a positional relationship based on that shown in the drawings, and are merely for convenience of description and to simplify the description, and do not indicate or imply that the apparatus or element in question must have a specific azimuth, be configured and operated in a specific azimuth, and therefore should not be construed as limiting the present application.
In this specification, each embodiment is described in a progressive manner, and identical and similar parts of each embodiment are all referred to each other, and each embodiment mainly describes differences from other embodiments.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In this application, unless specifically stated and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; the device can be mechanically connected, electrically connected and communicated; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
In this application, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
One embodiment of the present application provides an organoid culture device that is capable of gentle addition of reagents, simple to operate, and is not susceptible to contamination.
As shown in fig. 1 and 2, the apparatus includes:
the culture dish body 1, be provided with a plurality of bottom confined culture chamber 6 on the culture dish body 1.
Preferably, the culture chamber 6 includes a first culture chamber 7 and a second culture chamber 8 connected in this order from top to bottom, the horizontal sectional area of the second culture chamber 8 is smaller than that of the first culture chamber 7, and a step surface 9 is formed at the bottom of the first culture chamber 7.
Wherein, the organoid culture 13 is cultivated in the second cultivation room 8, and the second cultivation room 8 has a slightly smaller pore diameter than a normal dish, so that the nutrient solution needed for cultivation is relatively less, and the cultivation material is saved more. Also, during the actual culturing process, the applicant found that the organoid culture 13 cultured in the second culture chamber 8 had a shorter culture period and better reproducibility than the organoid culture cultured in the ordinary petri dish.
Preferably, the first culture chamber 7 and the second culture chamber 8 are cylindrical cavities, the radius of the bottom surface of the first culture chamber 7 is 1-2cm, and the radius of the bottom surface of the second culture chamber 8 is 0.3-0.9cm.
In this example, the radius of the bottom surface of the first culture chamber 7 was 1.74cm, and the radius of the bottom surface of the second culture chamber 8 was 0.88cm.
As shown in fig. 2, a culture dish cover is provided with a hollow liquid injection channel 3 penetrating through the cover body of the culture dish cover; the liquid injection channel 3 downwards extends out of the cover body of the culture dish to form a guide part 4, the bottom of the guide part 4 is an inclined plane with an elliptical opening, and the bottom end of the inclined plane is close to the side wall of the culture cavity 6; a filtering membrane 5 is arranged in the liquid injection channel 3, and the filtering membrane 5 is a bacterial filtering membrane.
Wherein, after entering the liquid injection channel 3, the liquid flows along the direction of the flow guiding part 4 and enters the culture cavity 6. The bottom of the flow guiding part 4 is provided with an elliptical inclined plane with an opening, so that liquid confluence can be better realized, and liquid splashing is avoided as much as possible.
In this embodiment, the radius of the pouring channel 3 is 0.1-0.2cm.
In this embodiment, water conservancy diversion portion 4 bottom is located step face 9 top, and the outer wall of water conservancy diversion portion 4 one side is hugged closely first cultivate room 7 lateral wall for liquid flows into step face 9 along first cultivate room 7 lateral wall, and then along step face 9 get into in the second cultivates room 8, compares liquid direct drip in organoid culture 13 top, and this application mode is milder, less to organoid culture 13 influence.
In addition, a bacterial filter membrane is fixed in the liquid injection channel 3. On the one hand, the liquid injection channel 3 can be used as a ventilation duct to realize gas exchange inside and outside the culture dish. In addition, in the gas exchange process, bacteria in the external environment are trapped by the bacteria filtering membrane, so that the sterile environment in the culture dish is ensured. On the other hand, the operator can realize the liquid feeding through the liquid injection channel 3, and can supplement culture solution or inject specific reagents without uncovering, thereby effectively avoiding the problem of bacteria contamination caused by frequently opening and closing a culture dish or deep-penetrating a culture cavity 6 chamber by a pipetting instrument.
Preferably, the inner side of the culture dish cover is provided with a sealing gasket 10, and the sealing gasket 10 is made of rubber.
In this embodiment, the inner side of the culture dish cover is provided with the sealing gasket 10, so that the arrangement can block the gas exchange between different culture cavities 6 in the same culture dish, and prevent the phenomenon that a plurality of culture cavities 6 around are infected after one of the culture cavities 6 is infected.
Preferably, the material of the culture dish cover and the culture dish body 1 is selected from one of glass, acrylic and ABS, HIPS, PP, PE.
In a preferred embodiment, the culture dish cover and the culture dish body 1 are made of transparent glass, and the culture dish made of transparent material is helpful for operators to observe the culture condition of the organoid culture 13 through the culture dish and judge the growth stage of the organoid culture 13.
As shown in fig. 3, the liquid injection channel 3 extends upward out of the culture dish cover body to form a sealing part 11.
Preferably, the organoid culture device further comprises a sealing cap 12, the sealing cap 12 being nested with the sealing portion 11.
Wherein, the person skilled in the art can choose to remove or close the sealing cover 12 according to the actual situation. The sealing cover 12 can be selectively covered when anaerobic culture is carried out, and the sealing cover 12 can be selectively removed when oxygen demand is large or exchange with external gas is needed.
The organoid culture device of this embodiment can be used to cover the seal cap 12 in a non-use state, ensuring an internal sterile environment.
Referring to fig. 2 and 3, in use, after the operator removes the sealing cap 12, the injector 14 or the pipette tip 15 for sucking the reagent is aligned with the opening of the injection channel 3, and the reagent is injected into the injection channel 3. The reagent flows along the liquid injection channel 3, enters the flow guide part 4 of the liquid injection channel 3 after being filtered by the filter membrane 5, is converged into one strand along the extension direction of the flow guide part 4, enters the first culture chamber 7, flows through the step surface 9 along the wall of the first culture chamber 7, flows into the second culture chamber 8, and realizes aseptic injection of the liquid reagent.
In this specification, each embodiment is described in a progressive manner, and identical and similar parts of each embodiment are all referred to each other, and each embodiment mainly describes differences from other embodiments. In particular, for system embodiments, since they are substantially similar to method embodiments, the description is relatively simple, as relevant to see a section of the description of method embodiments.
The foregoing is merely exemplary of the present application and is not intended to limit the present application. Various modifications and changes may be made to the present application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc. which are within the spirit and principles of the present application are intended to be included within the scope of the claims of the present application.
Claims (7)
1. A organoid culture device comprising:
the culture dish comprises a culture dish body, wherein a plurality of culture cavities with the bottoms being sealed are arranged on the culture dish body;
the culture dish cover is provided with a hollow liquid injection channel penetrating through the cover body of the culture dish cover; the liquid injection channel downwards extends out of the cover body of the culture dish cover to form a flow guide part, the bottom of the flow guide part is an inclined plane with an elliptical opening, and the bottom end of the inclined plane is close to the side wall of the culture cavity; a filtering membrane is arranged in the liquid injection channel, and the filtering membrane is a bacterial filtering membrane.
2. The organoid culture device of claim 1, wherein the culture chamber comprises a first culture chamber and a second culture chamber connected in sequence from top to bottom, the second culture chamber having a horizontal cross-sectional area smaller than the first culture chamber and a stepped surface formed at a bottom of the first culture chamber.
3. The organoid culture device of claim 2, wherein the first culture chamber and the second culture chamber are each cylindrical cavities, the radius of the bottom surface of the first culture chamber is 1-2cm, and the radius of the bottom surface of the second culture chamber is 0.3-0.9cm.
4. The organoid culture device of claim 1, wherein the fluid injection channel extends upwardly out of the culture dish cover to form a seal.
5. The organoid culture device of claim 4, further comprising a seal cap nested with the seal.
6. The organoid culture device of claim 1, wherein a gasket is provided inside the culture dish cover, and wherein the gasket is made of rubber.
7. The organoid culture device of claim 1, wherein the dish cover and the dish body are made of a material selected from the group consisting of glass, acrylic, ABS, HIPS, PP, PE.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320137986.1U CN220224189U (en) | 2023-01-16 | 2023-01-16 | Organ-like culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320137986.1U CN220224189U (en) | 2023-01-16 | 2023-01-16 | Organ-like culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220224189U true CN220224189U (en) | 2023-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202320137986.1U Active CN220224189U (en) | 2023-01-16 | 2023-01-16 | Organ-like culture device |
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| CN (1) | CN220224189U (en) |
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