CN215924968U

CN215924968U - Cell culture orifice plate convenient to trade liquid

Info

Publication number: CN215924968U
Application number: CN202121124441.4U
Authority: CN
Inventors: 闫文强; 敖英芳; 胡晓青; 时会娟; 周毕云
Original assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2021-05-24
Filing date: 2021-05-24
Publication date: 2022-03-01
Anticipated expiration: 2031-05-24

Abstract

The utility model discloses a cell culture pore plate convenient for liquid replacement, which comprises a bottom plate and a cover plate, wherein a plurality of cell culture pores are arranged on the bottom plate and are divided into a plurality of groups, the bottoms of each group of cell culture pores are mutually communicated, and a liquid drainage and injection device is arranged at the bottom of one cell culture pore positioned at the edge of the bottom plate in each group. The liquid discharging and injecting device comprises a liquid discharging pipe and a liquid guide pipe; one end of the liquid discharge pipe is communicated with the bottom of the cell culture hole, the other end of the liquid discharge pipe is connected with a liquid guide pipe, and the outlet of the liquid guide pipe is covered with a rubber cap. One cell culture hole at the edge of each group of cell culture holes on the cell culture pore plate is connected with a liquid discharge pipe, a liquid guide pipe and a rubber cap, and an injector punctures the rubber cap to suck out old culture solution and fill new culture solution, so that the culture solution is convenient to replace, and the risk of bacterial pollution in operation is reduced.

Description

Cell culture orifice plate convenient to trade liquid

Technical Field

The utility model belongs to the field of biological experimental vessels, and particularly relates to a cell culture pore plate convenient for liquid replacement.

Background

The cell culture well plate is a commonly used laboratory consumable for culturing cells, and consists of an independent bottom and an independent cover, culture well plates with corresponding specifications can be selected according to different experimental purposes, such as a hexawell plate, a dodecawell plate, a twenty-fourteen well plate and the like, and the material of the cell culture well plate is generally plastic, such as polystyrene material. Since cells need to be replaced with new culture solution periodically during the culture process, in the case of the current cell culture well plate commonly used in laboratories, the culture plate needs to be taken out of the cell culture box first and then transferred into an ultra-clean bench, and the cover plate needs to be removed in the whole process of replacing the culture solution, and the risk of bacterial contamination is undoubtedly increased by such a complicated series of operations. Once bacterial contamination occurs, the method results in the abandonment of the cell culture process and requires the cell culture process again, thereby increasing the experiment cost.

If an automatic liquid changing mode is adopted, the automatic liquid changing design of a cell culture dish in the existing design is complex, for example, in a patent with the patent application publication number of CN112646717A, the utility model discloses an intelligent dish cover for a stem cell culture dish convenient for adding nutrient solution, which comprises a cover body, wherein the top of the cover body is movably connected with a guide pipe, the top of the guide pipe is slidably connected with a valve, the inner wall of the guide pipe is fixedly connected with a driving wheel, the bottom of a pressure rod is movably connected with a connecting rod, the top of the push rod is movably connected with a pull rod, the top of the pull rod is movably connected with a driving shaft, and one side of the driving shaft, far away from the pull rod, is fixedly connected with a disinfection claw. This stem cell culture dish convenient to add nutrient solution is with intelligent dish lid, through moving the pipe, the pipe swings to vertical position, inserts nutrient solution pipe this moment, and the liquid pipe drives the valve and slides, and the action wheel rotates and drives the sliding tooth and rotate this moment, and the sliding tooth drives the reel and rotates, and the reel passes through the serving roll and drives the piston plate decline, and the rethread piston plate uses with the cooperation of swing door to the convenient effect of additive nutrient solution has been reached.

Therefore, the main drawback of the currently used cell culture well plate is that the cover plate needs to be removed all the way during the liquid exchange operation, which undoubtedly increases the risk of bacterial contamination; for the currently common cell culture pore plate, after the cover plate is removed, old culture solution in each culture pore needs to be sucked out one by one, and then culture solution is added one by one, so that the operation is complicated, the operation time is prolonged, and the infection risk is increased; the culture vessel with automatic liquid change has the disadvantages of complicated structure, obviously increased manufacturing cost and complicated operation in the prior patent.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems, the utility model provides a cell culture pore plate convenient for liquid replacement, wherein one cell culture pore at the edge of each group of cell culture pores on the cell culture pore plate is connected with a liquid discharge pipe, a liquid guide pipe and a rubber cap, and an injector punctures the rubber cap to suck out old culture liquid and fill new culture liquid, so that the replacement of the culture liquid is convenient, and the risk of bacterial pollution in operation is reduced.

The technical scheme of the utility model is as follows:

the utility model provides a cell culture orifice plate convenient to trade liquid, includes bottom plate and apron, be equipped with a plurality of cell culture holes on the bottom plate, a plurality of cell culture holes divide into a plurality of groups, and every group cell culture hole bottom communicates each other, and is located in every group a cell culture hole bottom at bottom plate edge is equipped with flowing back priming device.

The liquid discharging and injecting device comprises a liquid discharging pipe and a liquid guide pipe; one end of the liquid discharge pipe is communicated with the bottom of the cell culture hole, the other end of the liquid discharge pipe is connected with a liquid guide pipe, and the outlet of the liquid guide pipe is covered with a rubber cap.

The bottoms of each group of cell culture holes are communicated with each other through communicating pipes.

The diameter of the liquid discharge pipe is 1.0mm-2.5 mm.

A plurality of cell culture holes are arranged on the bottom plate in a matrix shape, and each group of cell culture holes are in a row or a column.

All the cell culture holes on the bottom plate are integrally a group, and the bottoms of the cell culture holes are communicated.

One side of the liquid discharge pipe and the bottom surface of the cell culture hole are positioned on the same plane.

A plurality of bulges are arranged on the edge of the upper surface of the bottom plate; the edge of the cover plate and the bottom plate is placed on the bulges when the cover plate and the bottom plate are covered, and gaps are arranged between the cover plate and the openings of the cell culture holes.

The utility model has the technical effects that:

1. according to the cell culture pore plate convenient for liquid replacement, the cell culture pores on the cell culture pore plate are divided into at least one group, generally, one row or one row is taken as a group, one cell culture pore at the edge of each group is connected with a liquid drainage pipe, a liquid guide pipe and a rubber cap, and an injector punctures the rubber cap to suck out old culture liquid and fill new culture liquid.

2. One side of the liquid discharge pipe and the bottom surface of the cell culture hole are positioned on the same plane, namely the liquid discharge pipe is arranged at the bottommost part of the cell culture hole, so that the purpose is to ensure that the small hole is positioned below the liquid level to the maximum extent, and then the original liquid is sucked out to the maximum extent.

3. The diameter of the liquid discharge pipe is 1.0mm-2.5mm, and liquid is changed more thoroughly. In the imbibition process, the liquid level height reduces gradually, if aperture diameter is too big, the liquid level just is less than the aperture top very fast, can suck together with the air, causes the imbibition incomplete, and liquid remains more. On the contrary, if the diameter of the small hole is small, the small hole can be ensured to be below the liquid level to the maximum extent, so that the liquid can be changed more thoroughly, and the original old culture solution can be sucked out by the liquid discharge pipe by about 95 percent.

4. The cover plate is placed on the bulge, and a gap is left between the cover plate and the bottom plate at the periphery, so that the cell culture hole is in a semi-closed state. The risk of preventing bacterial contamination has both been played, can realize cell culture hole and external gas exchange simultaneously, guarantees the downthehole gas of cell culture, and humidity keeps unanimous with in the incubator.

The cell culture pore plate convenient for liquid replacement is convenient for replacing the culture liquid, and reduces the risk of bacterial pollution in operation.

Drawings

Fig. 1 is a perspective view of the overall structure of the embodiment of the present invention.

Fig. 2 is a cross-sectional view of an embodiment of the present invention.

Fig. 3 is a cross-sectional view of an embodiment of the present invention.

In the figure, a base plate 1; a cover plate 2; a cell culture well 3; a liquid discharge pipe 4; a liquid guide tube 5; a rubber cap 6; a communicating pipe 7; and a protrusion 8.

Detailed Description

The present invention is further illustrated by the following examples.

As shown in fig. 1-3, a cell culture pore plate convenient for liquid replacement comprises a bottom plate 1 and a cover plate 2, 12 cell culture pores 3 arranged in a matrix shape are arranged on the bottom plate 1, the 12 cell culture pores 3 are divided into 3 groups, each row is a group, the bottoms of the cell culture pores 3 in each group are mutually communicated through a communicating pipe 7, a liquid drainage and injection device is arranged at the bottom of one cell culture pore 3 positioned at the edge of the bottom plate 1 in each group, the liquid drainage and injection device comprises a liquid drainage pipe 4 and a liquid guide pipe 5, one side of the liquid drainage pipe 4 and the bottom surface of the cell culture pore 3 are positioned on the same plane, and the diameter is 1.0mm-2.5 mm; one end of the liquid discharge pipe 4 is communicated with the bottom of the cell culture hole 3, the other end is connected with a liquid guide pipe 5, and the outlet of the liquid guide pipe 5 is covered with a rubber cap 6.

The corners of the edge of the upper surface of the bottom plate 1 are respectively provided with a bulge 8; when the cover plate 2 and the bottom plate 1 are covered, the edge is placed on the bulge 8, and a gap is ensured to be arranged between the cover plate 2 and the opening of the cell culture hole 3.

The principle and application of the utility model are as follows:

the embodiment is twelve hole cell culture orifice plate structure sketch map, contains independent bottom plate and apron, and on arranging the apron in the bottom plate during normal use, the bottom plate had certain clearance with the apron in the edge to can make the temperature, humidity and the gas concentration of cell culture hole inside and cell culture case keep unanimous. The cell culture hole 3 is in a semi-closed state, thereby preventing the risk of bacterial contamination and realizing the gas exchange between the cell culture hole and the outside.

The communicating pipe communicates a row of four cell culture holes at the bottom, and the diameter of the liquid discharge pipe is 2mm and is communicated with the bottom of the cell culture hole at the end part. The tip of the catheter to which the drainage tube is attached is threadably rotatably engaged with a closed rubber cap of a matching size. When cells are cultured in the cell culture holes and grow in an adherent manner, and the liquid needs to be changed, an alcohol cotton swab is firstly used for wiping the closed rubber cap, and an aseptic syringe is used for inserting the rubber cap, so that the old culture liquid is sucked out. Then, a syringe previously sucked with a fresh culture solution is inserted through the rubber cap and injected into the culture well. Through the operation, the liquid changing operation of the four cell culture holes can be realized at the same time under the condition of not removing the cover plate. For a commonly used twelve-well cell culture well plate, four cell culture wells in each row are changed by one drain pipe, and therefore, three drain pipes are required.

The cell culture pore plate can realize liquid change without moving the bottom plate or moving the cover plate, simultaneously realize liquid change operation in a plurality of cell culture pores, and avoid pollution risks caused by easy bacteria contact when the cover plate is uncovered and overlong liquid change time of a single cell culture pore in the operation process.

The above is, of course, only a specific application example of the present invention, and the scope of the present invention is not limited in any way. In addition to the above embodiments, the present invention may have other embodiments. All technical solutions formed by using equivalent substitutions or equivalent transformations fall within the scope of the present invention.

Claims

1. The utility model provides a cell culture orifice plate convenient to trade liquid, includes bottom plate (1) and apron (2), be equipped with a plurality of cell culture holes (3) on bottom plate (1), its characterized in that, a plurality of cell culture holes (3) divide into a plurality of groups, and every cell culture hole (3) bottom of group communicates each other, and is located in every group a cell culture hole (3) bottom at bottom plate (1) edge is equipped with drain injection device.

2. The well plate for cell culture convenient for changing liquid according to claim 1, characterized in that the liquid draining and injecting device comprises a liquid draining pipe (4) and a liquid draining pipe (5); one end of the liquid discharge pipe (4) is communicated with the bottom of the cell culture hole (3), the other end of the liquid discharge pipe is connected with a liquid guide pipe (5), and the outlet of the liquid guide pipe (5) is covered with a rubber cap (6).

3. A cell culture well plate facilitating liquid change according to claim 1, characterized in that the bottoms of each set of cell culture wells (3) are communicated with each other through a communicating tube (7).

4. The cell culture well plate facilitating fluid exchange according to claim 2, wherein the drain pipe (4) has a diameter of 1.0mm to 2.5 mm.

5. The cell culture well plate convenient for liquid change according to claim 1, characterized in that a plurality of cell culture wells (3) on the bottom plate (1) are arranged in a matrix, and each group of cell culture wells (3) is in a column or a row.

6. The cell culture well plate convenient for liquid exchange according to claim 1, characterized in that all the cell culture wells (3) on the bottom plate (1) are integrally formed into a group, and the bottoms of the wells are communicated.

7. The well plate for cell culture convenient for changing culture medium according to claim 2, wherein the side of the drain pipe (4) is on the same plane as the bottom surface of the well (3).

8. A cell culture well plate facilitating fluid exchange according to any of claims 1-7, wherein the bottom plate (1) is provided with a plurality of protrusions (8) at the edge of its upper surface; the edge of the cover plate (2) is placed on the bulges (8) when the cover plate and the bottom plate (1) are closed, and gaps are arranged between the cover plate (2) and the openings of the cell culture holes (3).