CN115088620A - American ginseng adventitious root tissue culture device - Google Patents

Abstract

The invention belongs to the related technical field of tissue culture of adventitious roots of American ginseng, in particular to a tissue culture device of the adventitious roots of American ginseng, after the motor is started, the motor can drive the transmission gears to rotate through the output shaft of the motor, the two transmission gears can synchronously rotate under the action of the chain, the two transmission gears can drive the two screw rods to rotate when rotating, when the screw rod rotates, the two adjacent left and right transmission plates can be driven to be far away from or close to each other, the main root of the American ginseng is clamped and extruded through the two adjacent left and right cutting frames, the main root of the American ginseng can be cut by the cutting edge, the American ginseng main root slice after cutting is placed in the containing groove, the operation is simple, the use is convenient, the American ginseng main root slice can be quickly cut in a large quantity, the operation is simple, the accuracy and the high efficiency are realized, and the practical performance is good.

Description

American ginseng adventitious root tissue culture device

Technical Field

The invention belongs to the technical field related to tissue culture of American ginseng adventitious roots, and particularly relates to a tissue culture device for American ginseng adventitious roots.

Background

American ginseng is native to the eastern United states and Canada, is a plant of the genus Panax of the family Araliaceae, and is most commonly used as a drug by root. Modern pharmacological studies show that American ginseng has the effects of regulating carbohydrate metabolism, enhancing immunity, delaying aging, resisting tumors, reducing blood pressure, preventing and treating cardiovascular diseases, protecting liver, enhancing learning ability and memory ability, benefiting intelligence and the like. Due to excessive mining for many years, the forest ecological environment in which the American ginseng depends on survival is seriously damaged, the wild American ginseng resources are exhausted, and most of the American ginseng for medical use at present is cultivated. However, the cultivation production period of the American ginseng is long, the occupied area is large, the quality of the American ginseng is easily influenced by climate, cultivation conditions and plant diseases and insect pests, and the supply cannot meet the market demand easily; the development of artificial American ginseng cultivation is also greatly limited by the problems of pesticide residue exceeding the standard, old ginseng land and the like.

The tissue culture method of American ginseng has the advantages of no limitation of growing seasons, no virus carrying, short culture period and the like, and is easy for industrial large-scale production, thereby having great development prospect. At present, when the American ginseng is subjected to tissue culture, firstly, adventitious roots are required to be induced, a main root of a cleaned mature American ginseng is sterilized, cut into slices with the width of 0.5-0.7cm, the length of 0.5-0.7cm and the thickness of 0.2-0.5mm, inoculated into an induction culture medium to induce the adventitious roots of the American ginseng, then, subculture of the adventitious roots is carried out in a liquid culture medium, and the obtained adventitious roots of the American ginseng are inoculated into the culture medium to be subjected to dark culture to obtain the adventitious roots of the American ginseng.

However, in the current tissue culture process of the adventitious roots of American ginseng, the main roots of mature American ginseng cannot be cut in a large quantity and accurately simply and quickly, which brings inconvenience to the tissue culture work of the adventitious roots, and when the adventitious roots are subcultured by using a liquid culture medium, the culture solution is inconveniently, quickly and uniformly injected into the culture medium, the adventitious roots of American ginseng are easily polluted by light in the injection process, in addition, the culture solution in the culture medium is also inconvenient to dump and take out the adventitious roots of American ginseng after the culture is completed, and the operation is troublesome.

In view of this, the invention provides an American ginseng adventitious root tissue culture device.

Disclosure of Invention

The invention aims to provide a tissue culture device for American ginseng adventitious roots, which is used for solving the problems that the tissue culture steps of the current American ginseng adventitious roots are more, the adventitious roots are easily subjected to light pollution, a culture medium needs to be replaced, and the operation is troublesome in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: an American ginseng adventitious root tissue culture device, which comprises: base and the support frame that is fixed in the base upper end, the top of base is provided with the workstation, the upper end of base is fixed with carries out the support column that supports to the workstation, the upside of workstation is provided with cultivates the dish, the upper end of workstation is fixed with carries out the overlap joint seat that supports to cultivateing the dish, the pot head of cultivateing the dish is equipped with the dish lid, the upside of dish lid is provided with and is used for carrying out the tray of placing to American ginseng main root, the outer wall of support frame rotates the cover and is equipped with the change, through dead lever fixed connection between change and the tray, the top fixed mounting of support frame has electronic hydraulic press, the lower extreme fixed mounting of electronic hydraulic press has cutting assembly for main root of American ginseng of placing to tray inside cuts.

Preferably, cultivate a plurality of boards that hold of inside fixedly connected with of dish, the cross sectional shape of holding the board is L shape, it highly degressive step by step to hold board outside-in bottom, cultivate the fixed cover of dish center department and be equipped with the flow tube, every the equal fixed cover in bottom of holding the board is equipped with casting tube, every the high difference of casting tube, the bottom fixedly connected with house steward of casting tube, house steward and casting tube are linked together, just house steward one end runs through flow tube inner wall and fixedly connected with suction pump, the upper end of casting tube is fixed with the filter screen, the inside check valve that is provided with of casting tube of filter screen downside.

Preferably, the inner view groove that is linked together with the culture dish inside is seted up to the cover plate outer wall, the upper end of cover plate rotates through the pivot to be connected with carries out confined flip to the inner view groove, the fixed cover in flip's upper end is equipped with the syringe that is linked together with the culture dish inside, the upper end screw thread cover of syringe is equipped with the end cover, flip's lateral wall is fixed with the handle that overturns the cover plate.

Preferably, the cutting assembly comprises an outer frame fixed at the lower end of the electric hydraulic press, the front side and the rear side of the outer frame are both rotationally connected with a screw rod, one end of the screw rod penetrates through the side wall of the outer frame and is fixed with a transmission gear, a chain is connected between the two transmission gears, and a motor for rotating the transmission gears is fixed on the side wall of the outer frame.

Preferably, cutting assembly still includes that the screw thread cover locates the type frame that returns on the outer wall of the left and right sides of lead screw, two the screw thread cover is equipped with two driving plates on the lead screw outer wall between the type frame that returns, the lower extreme of driving plate is fixed with cuts the mechanism, it cuts the fixed concatenation of frame by a plurality of to cut the mechanism.

Preferably, the cutting frame is provided with a containing groove inside, the containing groove is slidably sleeved with a push-pull plate, the outer wall of the push-pull plate is fixedly provided with a push-pull column which is slidably sleeved inside the cutting frame, the other end of the push-pull column is fixedly connected to the profiling frame, and a cutting edge is fixed at an end opening, far away from the profiling frame, of the cutting frame.

Preferably, the screw rods are equally divided into four sections, namely a first section, a second section, a third section and a fourth section from left to right, and the thread directions on the outer walls of the two adjacent sections of screw rods are opposite.

Preferably, the overlap joint seat is not fixed with the cultivation dish contact, the upper end of workstation is fixed with two fixed blocks, two fixedly connected with rotates the bull stick of cover locating cultivation dish inside between the fixed block.

Compared with the prior art, the invention provides a tissue culture device for adventitious roots of American ginseng, which has the following beneficial effects:

according to the cutting assembly, after the motor is started, the motor can drive the transmission gears to rotate through the output shafts of the motor, the two transmission gears can synchronously rotate under the action of the chain, when the two transmission gears rotate, the two lead screws can be driven to rotate, when the lead screws rotate, the two transmission plates which are adjacent to each other on the left and right can be driven to be away from or close to each other, the main root of the American ginseng is clamped and extruded through the two adjacent cutting frames on the left and right, the main root of the American ginseng can be cut by the aid of the cutting edge, and the cut main root slice of the American ginseng is placed in the accommodating groove;

the lead screw is divided into four sections, the first section, the second section, the third section and the fourth section are respectively arranged from left to right, the direction of threads on the outer wall of the two adjacent sections of lead screws is opposite, when the lead screw rotates, the moving direction of the cutting frame is opposite to that of a push-pull plate in the cutting frame, the fact that the push-pull plate cannot influence the placement of the American ginseng main root slices in the containing groove is ensured, and the push-pull plate can be used for pushing out and blanking the American ginseng main root slices in the containing groove, so that the structure is ingenious, and the use value is high;

thirdly, when the subculture of adventitious roots is carried out in a liquid culture medium, the prepared culture solution is injected into the culture tray through an injection tube, because the cross section of the containing plates is L-shaped, the heights of the containing plates are gradually reduced from outside to inside, the outermost containing plate overflows into the containing plate of the next layer after being filled, at the moment, the cover of the lower port of the circulating tube is opened, when the culture solution flows out from the lower port of the circulating tube, each containing plate is full of the culture solution, in the process, the tray cover can be always in a closed state, the light-shading effect is greatly improved, after the subculture of the adventitious roots of American ginseng is completed, a water suction pump is started to suck the header pipe, a one-way valve in the liquid discharging tube is opened, the culture solution accumulated in the containing plates flows into the header pipe through the liquid discharging tube and is sucked and discharged through the water suction pump, and after the culture solution is discharged, the water suction pump is closed, the adventitious roots are kept in the culture tray through the filter screen to be collected, the operation is simple, the convenience is brought, the working efficiency is greatly improved, the steps are reduced, and the exposure of the adventitious roots of the American ginseng in the culture tray is avoided.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention without limiting the invention in which:

FIG. 1 is a schematic plane structure of an apparatus for culturing adventitious roots of Panax quinquefolium according to the present invention;

FIG. 2 is a schematic view of the assembly structure of the cover plate according to the present invention;

FIG. 3 is a schematic view of the inner structure of the cover according to the present invention;

FIG. 4 is a schematic view of the internal structure of the culture tray according to the present invention;

FIG. 5 is a schematic sectional view of a culture tray according to the present invention;

FIG. 6 is a schematic view of a cutting assembly according to the present invention;

FIG. 7 is a schematic view of an assembly structure of the cutting frame according to the present invention;

in the figure: 1. a support frame; 2. an electro-hydraulic press; 3. cutting the assembly; 301. an outer frame; 302. a screw rod; 303. a back-shaped frame; 304. a drive plate; 305. cutting a frame; 306. a transmission gear; 307. a chain; 308. a motor; 309. a blade; 310. a receiving groove; 311. a push-pull plate; 312. pushing and pulling the column; 4. a tray; 5. fixing the rod; 6. rotating the ring; 7. a lap joint seat; 8. a culture tray; 9. a fixed block; 10. a support pillar; 11. a base; 12. a work table; 13. a tray cover; 14. a flow-through tube; 15. an end cap; 16. an injection tube; 17. a cover is turned; 18. a rotating rod; 19. a handle; 20. a holding plate; 21. an inner viewing groove; 22. a liquid discharging pipe; 23. a filter screen; 24. a header pipe; 25. a one-way valve; 26. a water pump.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-7, the present invention provides a technical solution: an American ginseng adventitious root tissue culture device, which comprises: a base 11 and a support frame 1 fixed on the upper end of the base 11, a workbench 12 is arranged above the base 11, a support column 10 for supporting the workbench 12 is fixed on the upper end of the base 11, a culture tray 8 is arranged on the upper side of the workbench 12 and used for culturing adventitious roots of American ginseng, an overlap joint seat 7 for supporting the culture tray 8 is fixed on the upper end of the workbench 12, a tray cover 13 is sleeved on the upper end of the culture tray 8, the tray cover 13 can slide up and down on the upper end of the culture tray 8 and also can rotate, the upper end of the culture tray 8 is shaded by the tray cover 13, a tray 4 for placing the main roots of American ginseng is arranged on the upper side of the tray cover 13, a rotary ring 6 is sleeved on the outer wall of the support frame 1 in a rotating manner, the rotary ring 6 is fixedly connected with the tray 4 through a fixing rod 5, the fixing rod 5 can rotate by the rotary ring 6, so as to rotate the position of the tray 4, the top fixed mounting of support frame 1 has electronic hydraulic press 2, and the lower extreme fixed mounting of electronic hydraulic press 2 has cutting element 3 for cut the inside American ginseng owner root of placing of tray 4.

Further, cultivate a plurality of boards 20 that hold of inside fixedly connected with of dish 8, the cross-sectional shape that holds board 20 is L shape, it descends progressively to hold board 20 outside-in bottom height, cultivate 8 center department fixed cover of dish and be equipped with flow tube 14, every bottom that holds board 20 is all fixed cover and is equipped with casting tube 22, a culture solution for holding board 20 inside discharges, every casting tube 22's high difference, casting tube 22's bottom fixedly connected with house steward 24, house steward 24 is linked together with casting tube 22, and house steward 24 one end runs through the 14 inner walls of flow tube and fixedly connected with suction pump 26, casting tube 22's upper end is fixed with filter screen 23, prevent that the gen-seng root after cutting from falling into casting tube 22, the inside check valve 25 that is provided with of casting tube 22 of filter screen 23 downside, the one side of filter screen 23 is kept away from to check valve 25 opening orientation.

Furthermore, the outer wall of the tray cover 13 is provided with an inner viewing groove 21 communicated with the inside of the culture tray 8, the inside of the culture tray 8 is observed through the inner viewing groove 21, the upper end of the tray cover 13 is rotatably connected with a flip cover 17 for sealing the inner viewing groove 21 through a rotating shaft, the upper end of the flip cover 17 is fixedly sleeved with an injection tube 16 communicated with the inside of the culture tray 8, the injection tube 16 is used for injecting culture solution or sterile water into the inside of the culture tray 8, the upper end of the injection tube 16 is sleeved with an end cover 15 through threads, and the side wall of the flip cover 17 is fixed with a handle 19 for turning the flip cover 17.

It should be noted that, cutting assembly 3 is including being fixed in frame 301 of electro-hydraulic press 2 lower extreme, frame 301 front and back side inside all rotates and is connected with lead screw 302, the one end of lead screw 302 runs through frame 301 lateral wall and is fixed with drive gear 306, be connected with chain 307 between two drive gear 306, the lateral wall of frame 301 is fixed with carries out pivoted motor 308 to drive gear 306, starter motor 308, motor 308 drives drive gear 306 through its output shaft and rotates, under the effect of chain 307, two drive gear 306 can synchronous rotation, when two drive gear 306 rotate, can drive two lead screws 302 and rotate.

It is worth noting that the cutting assembly 3 further comprises a circular frame 303 which is sleeved on the outer wall of the left side and the right side of the screw rod 302 in a threaded manner, two transmission plates 304 are sleeved on the outer wall of the screw rod 302 between the two circular frames 303 in a threaded manner, a cutting mechanism is fixed at the lower end of each transmission plate 304, and the cutting mechanisms are fixedly spliced by a plurality of cutting frames 305. The cutting frame 305 is provided with a containing groove 310 inside, the containing groove 310 is slidably sleeved with a push-pull plate 311, the outer wall of the push-pull plate 311 is fixed with a push-pull column 312 slidably sleeved inside the cutting frame 305, the other end of the push-pull column 312 is fixedly connected to the shaping frame 303, and a cutting edge 309 is fixed at an end opening of the cutting frame 305 far away from the shaping frame 303. The screw rods 302 are equally divided into four sections, namely a first section, a second section, a third section and a fourth section from left to right, and the thread directions on the outer walls of the two adjacent sections of screw rods 302 are opposite. When the screw 302 rotates, the two drive plates 304 adjacent to each other on the left and right can approach each other or move away from each other, the two loop frames 303 adjacent to each other on the left and right move away from each other or move close to each other, and the loop frames 303 and the drive plates 304 at the adjacent positions on the left and right move in opposite directions. When two adjacent left and right transmission plates 304 are close to each other, the main root of the American ginseng can be cut by two adjacent left and right cutting frames 305 and stored in the storage groove 310.

The lap joint seat 7 is not fixed with the contact of cultivateing the dish 8, and the upper end of workstation 12 is fixed with two fixed blocks 9, and fixedly connected with rotates the bull stick 18 that the cover was located cultivateing the inside of dish 8 between two fixed blocks 9, under the effect of bull stick 18, can overturn cultivateing the dish 8.

The working principle and the using process of the invention are as follows: when the cutting device is used for working, the main root of the mature American ginseng is cleaned and placed in the tray 4, at the moment, the tray 4 is positioned right below the sterilized cutting component 3, and the main root of the American ginseng is cut into slices with proper sizes by the cutting component 3. In the cutting process, the cutting component 3 is moved downwards to a proper position by the electric hydraulic press 2, the motor 308 is started, the motor 308 drives the transmission gear 306 to rotate through the output shaft of the motor 308, under the action of the chain 307, the two transmission gears 306 can synchronously rotate, when the two transmission gears 306 rotate, the two screw rods 302 can be driven to rotate, when the screw rod 302 rotates, the two adjacent left and right transmission plates 304 can be driven to move away from or close to each other, the American ginseng main root is clamped and extruded by the two adjacent cutting frames 305 on the left and right, and the main root of the American ginseng is cut by the cutting edge 309, so that the cut American ginseng main root slice is placed in the accommodating groove 310 (in the process, the moving direction of the cutting frames 305 is opposite to the moving direction of the push-pull plate 311 in the cutting frames 305, namely the push-pull plate 311 does not influence the placement of the American ginseng main root slice in the accommodating groove 310).

After cutting, the fixing rod 5 is rotated, so that the tray 4 is moved away from the upper side of the culture tray 8, at the moment, the tray cover 13 is detached from the upper end of the culture tray 8, the motor 308 rotates reversely, the left and right adjacent transmission plates 304 and the cutting frames 305 are driven to be away from each other, the left and right adjacent return frames 303 are close to each other, so that the push-pull plate 311 pushes out the thin slices in the containing groove 310, and cutting of the main root of the mature American ginseng is completed.

When the subculture of adventitious roots is carried out in the liquid medium, the prepared culture solution is injected into the outermost containing plate 20 through the injection pipe 16, the cross section of the containing plate 20 is L-shaped, and the heights of the bottoms of the containing plates 20 are gradually reduced from outside to inside, so that the outermost containing plate 20 overflows into the next containing plate 20 after being full, at the moment, the cover of the lower port of the circulating pipe 14 is opened, when the culture solution flows out from the lower port of the circulating pipe 14, the culture solution is indicated to be fully contained in each containing plate 20, the end cover 15 is closed for dark culture, the adventitious roots in the culture disc 8 can be observed through the inner observation groove 21 by the turnover cover 17, the light shielding performance is improved, the disc cover 13 can be rotated during observation, and whether the subculture is completed or not is judged.

After the adventitious root passage of the American ginseng is completed, the culture disc 8 is turned over through the rotating rod 18, and meanwhile, the water suction pump 26 is started to suck the header pipe 24, so that the one-way valve 25 in the liquid discharge pipe 22 is opened, and the culture liquid accumulated in the containing plate 20 flows down to the interior of the header pipe 24 through the liquid discharge pipe 22 and is sucked and discharged through the water suction pump 26. After the culture solution is discharged, the water suction pump 26 is turned off, the adventitious roots are kept in the culture tray 8 through the filter screen 23 and collected, the operation is simple and convenient, the working efficiency is greatly improved, the steps are reduced, and the adventitious roots of American ginseng in the culture tray 8 are prevented from being illuminated.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. The utility model provides an american ginseng adventitious root tissue culture device which characterized in that, it includes: the device comprises a base (11) and a support frame (1) fixed on the upper end of the base (11), wherein a workbench (12) is arranged above the base (11), a support column (10) for supporting the workbench (12) is fixed on the upper end of the base (11), a culture disc (8) is arranged on the upper side of the workbench (12), a lapping seat (7) for supporting the culture disc (8) is fixed on the upper end of the workbench (12), a disc cover (13) is sleeved on the upper end of the culture disc (8), a tray (4) for placing the main root of the American ginseng is arranged on the upper side of the disc cover (13), a rotating ring (6) is sleeved on the outer wall of the support frame (1), the rotating ring (6) is fixedly connected with the tray (4) through a fixing rod (5), and an electric hydraulic press (2) is fixedly installed on the top of the support frame (1), the lower extreme fixed mounting of electronic hydraulic press (2) has cutting assembly (3) for cut the inside American ginseng owner root of placing of tray (4).

2. The device for culturing the tissue of the American ginseng adventitious root according to claim 1, wherein: cultivate a plurality of boards (20) of holding of inside fixedly connected with of dish (8), the cross-sectional shape of holding board (20) is L shape, it is highly progressively reduced step by step to hold board (20) outside-in bottom, it is equipped with flow tube (14), every to cultivate fixed cover of dish (8) center department the equal fixed cover in bottom of holding board (20) is equipped with casting tube (22), every the height difference of casting tube (22), the bottom fixedly connected with house steward (24) of casting tube (22), house steward (24) are linked together with casting tube (22), just house steward (24) one end runs through flow tube (14) inner wall and fixedly connected with suction pump (26), the upper end of casting tube (22) is fixed with filter screen (23), casting tube (22) inside of filter screen (23) downside is provided with check valve (25).

3. The device of claim 1, wherein the device comprises: the interior groove of looking (21) that is linked together with cultivation dish (8) inside is seted up to dish lid (13) outer wall, the upper end of dish lid (13) is rotated through the pivot and is connected with and looks groove (21) in carrying out confined flip (17), the fixed cover in upper end of flip (17) is equipped with and cultivates syringe (16) that dish (8) inside is linked together, the upper end thread bush of syringe (16) is equipped with end cover (15), the lateral wall of flip (17) is fixed with carries out handle (19) that overturn to flip (17).

4. The device of claim 1, wherein the device comprises: cutting assembly (3) is including being fixed in frame (301) of electronic hydraulic press (2) lower extreme, frame (301) front and back side inside all rotates and is connected with lead screw (302), the one end of lead screw (302) runs through frame (301) lateral wall and is fixed with drive gear (306), two be connected with chain (307) between drive gear (306), the lateral wall of frame (301) is fixed with carries out pivoted motor (308) to drive gear (306).

5. The device for culturing the adventitious root tissue of the American ginseng according to claim 4, wherein: cutting assembly (3) still include the screw thread cover and locate lead screw (302) about return type frame (303) on the lateral wall, two the screw thread cover is equipped with two driving plates (304) on lead screw (302) outer wall between return type frame (303), the lower extreme of driving plate (304) is fixed with cuts the mechanism, cut the mechanism and cut frame (305) fixed concatenation by a plurality of.

6. The device of claim 5, wherein the device comprises: the cutting frame is characterized in that a containing groove (310) is formed in the cutting frame (305), a push-pull plate (311) is slidably sleeved in the containing groove (310), a push-pull column (312) slidably sleeved in the cutting frame (305) is fixed to the outer wall of the push-pull plate (311), the other end of the push-pull column (312) is fixedly connected to the shaping frame (303), and a cutting edge (309) is fixed to an end opening, far away from the shaping frame (303), of the cutting frame (305).

7. The device of claim 6, wherein the device comprises: the screw rods (302) are equally divided into four sections, namely a first section, a second section, a third section and a fourth section from left to right, and the directions of the threads on the outer walls of the two adjacent sections of screw rods (302) are opposite.

8. The device for culturing the tissue of the American ginseng adventitious root according to claim 1, wherein: overlap joint seat (7) and cultivation dish (8) contact are not fixed, the upper end of workstation (12) is fixed with two fixed blocks (9), two fixedly connected with rotates the cover and locates the bull stick (18) of cultivating the dish (8) inside between fixed block (9).

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