CN210826176U

CN210826176U - Microbial community total number detection device

Info

Publication number: CN210826176U
Application number: CN201921728330.7U
Authority: CN
Inventors: 刘亭; 张煜彬; 薛维娜; 兰燕宇; 李勇军; 王永林; 王爱民
Original assignee: Guizhou Medical University
Current assignee: Guizhou Medical University
Priority date: 2019-10-15
Filing date: 2019-10-15
Publication date: 2020-06-23
Anticipated expiration: 2029-10-15

Abstract

The utility model discloses a microorganism bacterial colony total number detection device, including the incubator, the mounting panel that two symmetries of lower extreme fixedly connected with of incubator set up, two be equipped with the first extension board and the second extension board that the level set up between the mounting panel, be equipped with secondary culture apparatus on the first extension board, be equipped with the detection ware on the second extension board. The utility model discloses can adjust the extraction degree of depth of culture medium as required, the quality of culture medium is higher, does benefit to the cultivation of bacterial colony and detects.

Description

Microbial community total number detection device

Technical Field

The utility model relates to a little biological detection technical field especially relates to a microorganism colony total number detection device.

Background

A colony is a group of daughter cells, which are grown and propagated to a certain extent on the surface or inside of a suitable solid medium from a single microbial cell or a stack of cells of the same species, and which are characterized by a certain morphology, structure, etc., and are visible to the naked eye around a mother cell. The colony characteristics of various microorganisms formed under certain conditions, such as size, shape, edge, surface, texture, color and the like, have certain stability and are important basis for measuring the purity of strains and identifying the strains. The colony characteristics are closely related to the morphological and structural characteristics of the thallus of the microorganism. For example, bacteria and yeasts do not form hyphae, and colonies only grow on the surface of a solid culture medium and are not tightly combined with the culture medium, so that all the colonies can be picked up by using an inoculation tool; most of the thallus of actinomycetes and mould is differentiated into vegetative hyphae and propagation hyphae, the vegetative hyphae deeply penetrate into a culture medium to absorb nutrition, the culture medium has the characteristics of tight combination with the culture medium, difficult lifting and the like, and a detection device is required to be used for detecting after the microbial colony is cultured;

the existing device for detecting the total number of the microbial colonies can not adjust the extraction depth of a culture medium according to use requirements, the quality of the culture medium is poor, and the culture detection of the colonies is not facilitated.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problems in the prior art and providing a total number detection device for microbial colonies.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a microorganism fungus colony total number detection device, includes the incubator, the mounting panel that two symmetries of lower extreme fixedly connected with of incubator set up, two be equipped with the first extension board and the second extension board that the level set up between the mounting panel, be equipped with secondary culture apparatus on the first extension board, be equipped with the detection ware on the second extension board.

Preferably, the upper end of incubator is equipped with the inlet pipe, and the inlet pipe runs through the incubator setting, the both sides lateral wall of incubator all is equipped with the electrical heating stick, and two electrical heating sticks all with the inside intercommunication of incubator, the upper end of incubator is equipped with the rubber head burette that two symmetries set up, two the rubber head burette all with the upside lateral wall activity joint of incubator.

Preferably, secondary culture apparatus includes the erlenmeyer flask, the upper end of erlenmeyer flask is equipped with the check valve, and the upper end and the inside intercommunication of incubator of erlenmeyer flask, be equipped with the flexible extraction tube that two symmetries set up in the erlenmeyer flask, two equal fixedly connected with L type branch pipes in one side that flexible extraction tube carried on the back mutually, and two L type branch pipes all run through the lateral wall of erlenmeyer flask and are connected with conveyor.

Preferably, two the equal fixedly connected with cooler bin of the relative lateral wall of mounting panel, and two cooler bins are located between first extension board and the second extension board, two the equal fixedly connected with refrigerator of the relative lateral wall of cooler bin, and the output of two refrigerators all communicates with the cooler bin is inside, two the equal fixedly connected with conveyer pipe of lower extreme of cooler bin, and two conveyer pipes are located two detection wares respectively directly over, two conveyor all includes the delivery pump, and two delivery pumps all with the downside lateral wall fixed connection of first extension board, two the output of delivery pump all communicates with the cooler bin is inside.

Preferably, two the equal fixedly connected with first sterilamp of one side lateral wall that the mounting panel is relative, and the downside lateral wall fixedly connected with second sterilamp of first extension board.

Preferably, two flexible extraction tube all includes the first branch pipe and the second branch pipe of vertical setting, two the upper end of first branch pipe is the closed setting, and the equal fixedly connected with telescopic cylinder in upper end of two first branch pipes, two telescopic cylinder's output all runs through the upper end of first branch pipe and with second branch pipe fixed connection.

The utility model discloses well beneficial effect as follows:

1. adding lecithin and distilled water into an incubator through a feeding pipe, opening an electric heating rod to heat the distilled water to dissolve and mix the lecithin and the distilled water, adjusting the pH value of the lecithin mixed solution to 7.2-7.3 by using two rubber-head droppers, and respectively carrying out ultraviolet sterilization on a conical flask and a cooling box by using a first ultraviolet sterilization lamp and a second ultraviolet sterilization lamp;

2. open the check valve, lecithin mixed liquid gets into the erlenmeyer flask in the sediment cultivation, telescopic cylinder drives the second branch pipe and moves in first branch pipe, adjusts the degree of depth of drawing the culture medium, and the delivery pump passes through L type branch pipe and the cooperation of flexible extraction tube, cools off the save in carrying the culture medium to the cooler bin, when needs are tested, carries the culture medium to detecting the ware via the conveyer pipe, and it can to add the bacterial strain and detect the count after cultivateing.

Drawings

FIG. 1 is a schematic structural view of a total number of microbial colonies detection device provided by the present invention;

FIG. 2 is a schematic structural view of the retractable extraction tube of the total number of microbial colonies detection device of the present invention.

In the figure: the device comprises an incubator 1, a mounting plate 2, a first support plate 3, a second support plate 4, a detection dish 5, a feeding pipe 6, an electric heating rod 7, a rubber head dropper 8, a conical flask 9, a check valve 10, a branch pipe 11L-shaped, a cooling box 12, a refrigerator 13, a delivery pipe 14, a delivery pump 15, a first ultraviolet germicidal lamp 16, a second ultraviolet germicidal lamp 17, a first branch pipe 18, a second branch pipe 19 and a telescopic cylinder 20.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1, a total amount of microbial colonies detection device, comprising an incubator 1, wherein the upper end of the incubator 1 is provided with a feed pipe 6, the feed pipe 6 penetrates through the incubator 1, the two side walls of the incubator 1 are respectively provided with an electric heating rod 7, the two electric heating rods 7 are respectively communicated with the inside of the incubator 1, the upper end of the incubator 1 is provided with two symmetrically arranged rubber head droppers 8, the two rubber head droppers 8 are movably clamped with the upper side wall of the incubator 1, acid liquid and alkaline liquid are respectively arranged in the two rubber head droppers 8, the side wall of one side opposite to the two mounting plates 2 is respectively fixedly connected with a first ultraviolet sterilizing lamp 16, the side wall of the lower side of the first support plate 3 is fixedly connected with a second ultraviolet sterilizing lamp 17, lecithin and distilled water are added into the incubator 1 through the feed pipe 6, the distilled electric heating rod 7 is turned on to heat the distilled water, so that the lecithin and the distilled, the pH value of the lecithin mixed solution is adjusted to 7.2-7.3 by using two rubber-head droppers 8, and a first ultraviolet sterilizing lamp 16 and a second ultraviolet sterilizing lamp 17 can respectively carry out ultraviolet sterilization on the conical flask 9 and the cooling box 12;

the lower end of the incubator 1 is fixedly connected with two symmetrically arranged mounting plates 2, a first support plate 3 and a second support plate 4 which are horizontally arranged are arranged between the two mounting plates 2, a secondary culture device is arranged on the first support plate 3, a detection dish 5 is arranged on the second support plate 4, the secondary culture device comprises a conical flask 9, a check valve 10 is arranged at the upper end of the conical flask 9, the upper end of the conical flask 9 is communicated with the inside of the incubator 1, two symmetrically arranged telescopic extraction pipes are arranged in the conical flask 9, L-shaped branch pipes 11 are fixedly connected to the opposite sides of the two telescopic extraction pipes, the two L-shaped branch pipes 11 penetrate through the side wall of the conical flask 9 and are connected with a conveying device, cooling boxes 12 are fixedly connected to the side wall of one side opposite to the two mounting plates 2, the two cooling boxes 12 are positioned between the first support plate 3 and the second support plate 4, and refrigerators 13 are fixedly connected to the side wall of, the output ends of the two refrigerators 13 are communicated with the interior of the cooling boxes 12, the lower ends of the two cooling boxes 12 are fixedly connected with conveying pipes 14, and the two conveying pipes 14 are respectively positioned right above the two detection dishes 5;

the two conveying devices comprise conveying pumps 15, the two conveying pumps 15 are fixedly connected with the lower side wall of the first support plate 3, the output ends of the two conveying pumps 15 are communicated with the interior of the cooling box 12, the two telescopic extraction pipes comprise a first branch pipe 18 and a second branch pipe 19 which are vertically arranged, the upper ends of the two first branch pipes 18 are sealed, the upper ends of the two first branch pipes 18 are fixedly connected with telescopic air cylinders 20, the output ends of the two telescopic air cylinders 20 penetrate through the upper ends of the first branch pipes 18 and are fixedly connected with the second branch pipes 19, the check valves 10 are opened, lecithin mixed liquid enters the conical flask 9 for precipitation culture, the telescopic air cylinders 20 drive the second branch pipes 19 to move in the first branch pipes 18, the depth of the extracted culture medium is adjusted, the conveying pumps 15 are matched with the telescopic extraction pipes through L-shaped branch pipes 11, the culture medium is conveyed to the cooling box 12 for cooling and storage, when the experiment is needed, the culture medium is conveyed to the detection dish 5 through the conveying pipe 14, and the strains are added for culture and then are detected and counted.

The utility model discloses in, in passing through inlet pipe 6 with lecithin and distilled water and adding incubator 1, open electrical heating rod 7 and heat distilled water, make lecithin and distilled water dissolve and mix, use two rubber head burettes 8 to debug the pH value of lecithin mixed liquid to 7.2-7.3, open and control valve 10, lecithin mixed liquid deposit in getting into erlenmeyer flask 9 and cultivate, telescopic cylinder 20 drives second branch pipe 19 and moves in first branch pipe 18, adjust the degree of depth of drawing the culture medium, delivery pump 15 passes through L type branch pipe 11 and the cooperation of flexible extraction tube, carry the culture medium and cool off in cooling tank 12 and preserve, when needs are tested, carry the culture medium to detection ware 5 via conveyer pipe 14, it can to add the bacterial strain and detect the count after cultivateing.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a microorganism fungus colony total number detection device, includes incubator (1), its characterized in that, two mounting panel (2) that the lower extreme fixedly connected with of incubator (1) set up symmetrically, two be equipped with first extension board (3) and second extension board (4) that the level set up between mounting panel (2), be equipped with secondary culture apparatus on first extension board (3), be equipped with on second extension board (4) and detect ware (5).

2. The total microbial colony number detection device according to claim 1, wherein the upper end of the incubator (1) is provided with a feed pipe (6), the feed pipe (6) is arranged to penetrate through the incubator (1), the two side walls of the incubator (1) are respectively provided with an electric heating rod (7), the two electric heating rods (7) are respectively communicated with the inside of the incubator (1), the upper end of the incubator (1) is provided with two symmetrically arranged rubber head droppers (8), and the two rubber head droppers (8) are respectively movably clamped with the upper side wall of the incubator (1).

3. The total microbial colony number detection device according to claim 1, wherein the secondary culture device comprises a conical flask (9), a check valve (10) is arranged at the upper end of the conical flask (9), the upper end of the conical flask (9) is communicated with the inside of the incubator (1), two symmetrically arranged telescopic extraction pipes are arranged in the conical flask (9), an L-shaped branch pipe (11) is fixedly connected to one side of each of the two telescopic extraction pipes, which is opposite to the other side of each of the two telescopic extraction pipes, and the two L-shaped branch pipes (11) penetrate through the side wall of the conical flask (9) and are connected with a conveying device.

4. The total microorganism colony detection device according to claim 3, wherein a cooling box (12) is fixedly connected to the side wall of one of the two mounting plates (2) opposite to each other, two cooling boxes (12) are positioned between the first support plate (3) and the second support plate (4), the side walls of one side of the two cooling boxes (12) opposite to each other are fixedly connected with a refrigerator (13), the output ends of the two refrigerators (13) are communicated with the interior of the cooling boxes (12), the lower ends of the two cooling boxes (12) are fixedly connected with conveying pipes (14), and the two conveying pipes (14) are respectively positioned right above the two detection dishes (5), the two conveying devices respectively comprise a conveying pump (15), and the two delivery pumps (15) are fixedly connected with the side wall of the lower side of the first support plate (3), and the output ends of the two delivery pumps (15) are communicated with the interior of the cooling box (12).

5. The apparatus for detecting the total number of microbial colonies as claimed in claim 1, wherein a first UV sterilization lamp (16) is fixedly connected to each of the opposite side walls of the two mounting plates (2), and a second UV sterilization lamp (17) is fixedly connected to the lower side wall of the first support plate (3).

6. The total microbial colony number detection device according to claim 3, wherein each of the two telescopic extraction pipes comprises a first branch pipe (18) and a second branch pipe (19) which are vertically arranged, the upper ends of the two first branch pipes (18) are both arranged in a closed manner, telescopic cylinders (20) are fixedly connected to the upper ends of the two first branch pipes (18), and the output ends of the two telescopic cylinders (20) penetrate through the upper ends of the first branch pipes (18) and are fixedly connected with the second branch pipes (19).