CN202369595U - Cell culture flask - Google Patents

Abstract

A cell culture bottle, which relates to a cell culture vessel, to solve the problem that the existing cell culture bottle has poor gas permeability, which is not conducive to cell growth, and when the existing cell culture bottle is used to observe the cultured cells, the culture solution needs to be taken out and placed on a carrier. On glass slides, placed under a microscope, it is easy to contaminate the culture medium containing cells in the culture bottle, and the operation is cumbersome, which increases the difficulty of the experimenter's work. It includes a bottle body and a bottle cap, and it also includes a horizontal section and a semi-permeable membrane. The side wall of the bottle body is provided with a horizontal section and the two are integrated, and the bottom end surface of the horizontal section is flush with the bottom surface of the bottle body , the shape of the horizontal section is a flat cuboid, the bottle body is composed of a main body, a bottleneck and a bottleneck, the upper end of the main body is sequentially provided with a bottleneck and a bottleneck from bottom to top, and a semi-permeable membrane is covered on the bottleneck of the bottle body, The semipermeable membrane is compressed by a bottle cap connected with the bottle mouth of the bottle body, and at least one vent hole is arranged on the top of the bottle cap. The utility model is used for cell culture.

Description

a cell culture flask

technical field

The utility model relates to a cell culture vessel.

Background technique

Cell culture refers to the growth of cells outside the body under artificial conditions. The cells grown during the culture process no longer differentiate into tissues. It is the basis and important means of production in biotechnology. The purpose is to harvest the cells themselves or to obtain cellular metabolites. Cell culture flasks provide the necessary environment for the growth of cells. The culture flasks of the prior art have poor air permeability and are unfavorable for cell growth. After culturing cells in culture flasks used in research, biological experiment teaching, and medical clinical experiments, the culture solution needs to be taken out and placed on a glass slide for observation under a microscope. It is easy to contaminate the culture solution containing cells in the culture bottle, and the operation is troublesome. Increased the difficulty of the experimenter's work.

Utility model content

The purpose of this utility model is to solve the problem that the existing cell culture bottle has poor air permeability, which is not conducive to cell growth, and when using the existing cell culture bottle to observe the cultured cells, it is necessary to take out the culture solution and place it on a glass slide for observation under a microscope , it is easy to contaminate the culture solution containing cells in the culture bottle, and the operation is cumbersome, which increases the difficulty of the experimenter's work, so as to provide a cell culture bottle.

The technical scheme adopted by the utility model to solve the above problems is: a cell culture bottle of the utility model includes a bottle body and a bottle cap, and it also includes a horizontal section and a semi-permeable membrane. The side wall of the bottle body is provided with a horizontal and the two are integrated, and the bottom surface of the horizontal section is flush with the bottom surface of the bottle body. The shape of the horizontal section is a flat cuboid. The bottle body is composed of a main body, a bottleneck and a bottleneck. The top is provided with a bottleneck and a bottleneck in sequence, and the semipermeable membrane covers the bottleneck of the bottle body, and the semipermeable membrane is compressed by a bottle cap connected to the bottleneck of the bottle body, and at least one vent hole is arranged on the top of the bottle cap.

The beneficial effects of the utility model are: the utility model has dual-purpose functions of cell culture and observation. The semi-permeable membrane of the utility model has a good gas permeability function, and the top of the bottle cap is provided with a vent hole, which is conducive to cell ventilation, meets the needs of oxygen and carbon dioxide, and is beneficial to cell growth, with fast cell proliferation and good culture effect; Place the flat rectangular parallelepiped horizontal section under the microscope to directly observe the cells being cultured without taking out the culture solution containing the cells and placing it on a glass slide for observation, effectively avoiding contamination of the culture solution in the culture bottle , the operation is simple and convenient, saves time and effort, and reduces the difficulty of the experimenter's work. At the same time, the semi-permeable membrane of the utility model can effectively prevent external microorganisms from entering the culture bottle, and there is no pollution in the bottle, so that the growth and reproduction of cells are in the best state. , high cell viability. The utility model is suitable for cell culture for scientific research, cell culture for school biology course experiments, and cell culture for medical clinical experiments.

Description of drawings

Fig. 1 is a front view of the overall structure of the utility model, and Fig. 2 is a main sectional view of the connection structure between the bottle cap and the bottle body.

Detailed ways

Specific Embodiment 1: This embodiment is described in conjunction with FIG. 1 and FIG. 2. A cell culture bottle of this embodiment includes a bottle body 2 and a bottle cap 3, and it also includes a horizontal section 1 and a semipermeable membrane 4. The bottle The side wall of the body 2 is provided with a horizontal section 1 and the two are integrated, and the bottom surface of the horizontal section 1 is flush with the bottom surface of the bottle body 2, the shape of the horizontal section 1 is a flat cuboid, and the bottle body 2 is made of The main body 2-1, the bottleneck 2-2 and the bottleneck 2-3 are composed, the upper end of the main body 2-1 is provided with the bottleneck 2-2 and the bottleneck 2-3 in sequence from bottom to top, and the semipermeable membrane 4 covers the bottom of the bottle body 2. On the bottle mouth 2-3, the semipermeable membrane 4 is compressed by the bottle cap 3 connected to the bottle mouth 2-3 of the bottle body 2, and at least one vent hole 3-1 is arranged on the top of the bottle cap 3.

The semipermeable membrane described in this embodiment has the function of ventilating and preventing external microorganisms from entering the culture bottle. The semipermeable membrane can be made of medical polymer materials such as polytetrafluoroethylene or silicon rubber; the horizontal section and the bottle body of this embodiment It can be made of composite materials such as polystyrene or glass.

Specific Embodiment 2: This embodiment is described with reference to FIG. 1 . The shape of the main body 2 - 1 described in this embodiment is a cylinder or a cuboid. Such setting meets the design requirements and actual needs. Others are the same as in the first embodiment.

Embodiment 3: This embodiment is described in conjunction with FIG. 2 . The bottle cap 3 of this embodiment is screwed to the bottle mouth 2-3 of the bottle body 2 . With such setting, the connection is efficient and reliable, meeting design requirements and actual needs. Others are the same as in the first or second embodiment.

Specific Embodiment 4: This embodiment is described with reference to FIG. 1 and FIG. 2 . The shape of the vent hole 3 - 1 in this embodiment is a circular hole or a square hole. Such setting meets the design requirements and actual needs. Others are the same as in the first or second embodiment.

work process

Add the culture solution into the culture bottle, cover the bottle mouth with a semi-permeable membrane, and press the semi-permeable membrane through the bottle cap connected to the bottle mouth to form a breathable cell culture environment in the culture bottle. When observing the cells, it is sufficient to place the horizontal section in the shape of a flat rectangular parallelepiped under a microscope for observation.

Claims (4)

1. Tissue Culture Flask; It comprises bottle (2) and bottle cap (3); It is characterized in that: it also comprises horizontal section (1) and semi-permeable membranes (4), and the sidewall of described bottle (2) is provided with horizontal section (1) and the two processes one, and the bottom face of horizontal section (1) flushes with the bottom surface of bottle (2); Described horizontal section (1) be shaped as the flat-section rectangular parallelepiped; Bottle (2) is made up of main body (2-1), bottleneck (2-2) and bottleneck (2-3), and the upper end of main body (2-1) is disposed with bottleneck (2-2) and bottleneck (2-3) from the bottom to top, and semi-permeable membranes (4) covers on the bottleneck (2-3) of bottle (2); Semi-permeable membranes (4) compresses through the bottle cap (3) that is connected with the bottleneck (2-3) of bottle (2), and the top of bottle cap (3) is provided with at least one ventilating pit (3-1).

2. a kind of Tissue Culture Flask according to claim 1 is characterized in that: described main body (2-1) be shaped as right cylinder or rectangular parallelepiped.

3. a kind of Tissue Culture Flask according to claim 1 and 2 is characterized in that: bottle cap (3) is threaded with the bottleneck (2-3) of bottle (2).

4. a kind of Tissue Culture Flask according to claim 1 and 2 is characterized in that: described ventilating pit (3-1) be shaped as circular port or square opening.

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