CN2786131Y

CN2786131Y - Liquid strain incubator

Info

Publication number: CN2786131Y
Application number: CN 200520030757
Authority: CN
Inventors: 李俊贤
Original assignee: Individual
Current assignee: Individual
Priority date: 2005-05-25
Filing date: 2005-05-25
Publication date: 2006-06-07
Anticipated expiration: 2015-05-25

Abstract

The utility model relates to a liquid strain incubator. Incubation media are arranged in a container, and a container mouth is sealed by a bottle stopper which is provided with three vertical through holes. The through holes are respectively provided with an air inlet pipe, an exhaust pipe and a liquid inlet pipe. The utility model is characterized in that the inner end opening of the air inlet pipe is led into the incubation media, and the air inlet pipe outside the container is connected with a valve, a filter, a flow meter and an air pump in sequence. The inner end opening of the container of the exhaust pipe is higher than the liquid surface of the incubation media, and a valve and a filter are arranged outside the container in sequence. The inner end opening of the liquid outlet pipe is inserted into the bottom of the container, and the valve is arranged outside the container of the liquid outlet valve. The utility model has the advantages that various bacteria and germs can be effectively filtered, and the success ratio is more than 98 percents. The strain incubation has easy control, the effect is good, the cost is low, therefore the liquid strain incubator is easy to accept by farmers.

Description

The strain cultivation device

(1) technical field: the utility model relates to edible mashroom cultivating, further is the equipment of simple and easy production edible fungi liquid strain.

(2) background technology: utilize the liquid strain cultivation edible mushrooms, have the saving of labor, save time, early receipts, high yield, be fit to characteristics such as mechanize production.But in the market strain cultivation device or fermentor tank, often investment is big, and generally more than several thousand yuan, the mechanical means that some are large-scale can reach several ten thousand yuan or higher, and general peasant is difficult for accepting.

(3) summary of the invention: the purpose of this utility model provides a kind of easy and simple to handle, the simple and easy strain cultivation device that cost is low.

The purpose of this utility model realizes by following scheme: the strain cultivation device, substratum is housed in the container, vessel port is passed through plug seal, stopper vertically has three through holes, be equipped with inlet pipe, vapor pipe and drain pipe in the through hole respectively, be characterized in: the inlet pipe inner port feeds in the substratum, and the part of inlet pipe outside container is provided with valve, strainer and air pump successively; The vapor pipe inner port is higher than the substratum liquid level, and the part of vapor pipe outside container is provided with valve and strainer successively; The drain pipe inner port is inserted container bottom, and the part of drain pipe outside container is provided with valve.

The outstanding advantage of the utility model is: 1, adopt double-deck millipore filtration, and the various bacteriums of filtering, germ effectively, to influence various moulds such as green mold that Edible Fungi grows, aspergillus, wooden mould, Mucor, Neurospora, success ratio is more than 98% especially.2, utilize the liquid spawn of this device fabrication, the amount of producing than shaking table is big, once can produce liquid spawn 0.5-50 liter.3, spawn culture is easy to control, and is effective, can be used to transfer original seed or cultivar, but also direct inoculation cultivating bag.4, cost is low, less investment, and instant effect, about hundred yuan of complete machine costs are easily accepted by the peasant.

(4) description of drawings: accompanying drawing 1 is the utility model structural representation, and Fig. 2 is the utility model filter structure schematic representation.

(5) embodiment:, the utility model is done further narration below in conjunction with embodiment.

Embodiment 1: as shown in Figure 1, the material of container (1) can adopt a kind of of stainless steel, enamel, plastics, glass, and volume generally can be selected the 0.5-50 liter for use, and vessel port (2) is by stopper (3) sealing, and stopper (3) can adopt white rubber material.Substratum (4) is housed in the container (1), stopper (3) vertically is provided with three through holes, be equipped with inlet pipe (5), vapor pipe (6) and drain pipe (7) in the through hole respectively, inlet pipe (5) is located at the central authorities of stopper (3), the port of inlet pipe (5) in container (1) connects and disperses ball (8), disperse ball (8) can place the central authorities of substratum (4) or central authorities position on the lower side, disperseing ball (8) is spherical shell shape, it also can be tubbiness, have some uniform small pores above, by disperseing the effect of ball (8), can make even air ground be scattered in substratum (4) everywhere.On the inlet pipe (5) outside container (1), be equipped with valve (9), strainer (10), under meter (11) and air pump (12) successively.

Wherein, whether valve (9) control ventilates.

Strainer (10) structure as shown in Figure 2, two intermediate plates (10-1) middle part is provided with ventilating pit (10-2), ventilating pit (10-2) communicates with pipe connecting (10-3), intermediate plate (10-1) is provided with connecting hole (10-4) all around, fix by screw, there is 0.8 micron in the folder, 0.22 micron double-deck millipore filtration (10-5), wherein 0.8 micron millipore filtration (10-5) is towards air pump (12).The periphery of millipore filtration (10-5) is provided with liner (10-7) with diaphragm seal (10-6) sealing between 0.22 micron filter membrane (10-5) and diaphragm seal (10-6), liner (10-7) can be made of the material of flexibility, as hospital gauze etc.Pipe connecting (10-3) is connected with inlet pipe (5), to realize the filtration of air, the protection bacterial classification.

The purpose of under meter (11) is to show air flow, and for what of air flow, the different times in substratum of different quantities (4) and the system bacterium process has different requirements.General air flow is controlled at 0.3-1 liter/LPM (Liters Per Minute), and promptly the substratum of each liter (4) per minute feeds the air that 0.3-1 rises.

Can be after air pump (12) energising to inlet pipe (5) bubbling air.

The part of vapor pipe (6) outside container (1) is provided with valve (13) and strainer (14), and strainer (14) structure is identical with strainer (10).The port of vapor pipe (6) in container (1) is higher than substratum (4) liquid level, by the effect of inlet pipe (5) and vapor pipe (6), keeps the circulation of the interior air of container (1).

The part of drain pipe (7) outside container (1), be provided with valve (15), the conducting of may command drain pipe (7), thus avoid contacting of air and substratum (4), the lower port of drain pipe (7) is inserted the bottom of container (1), the liquid spawn in the container (1) all can be extracted out.During drawing liquid body bacterial classification, start air pump (12) earlier, container (1) is ventilated by inlet pipe (5), again the valve (13) of vapor pipe (6) is closed, and open the valve (15) of drain pipe (7), under the effect of gaseous tension, liquid spawn is flowed out from drain pipe (7) in container (1).

The tubing of inlet pipe (5), vapor pipe (6), drain pipe (7) can adopt Glass tubing, stainless steel tube and plastic rigid pipe.

During use, substratum (4) in the container (1) is formulation routinely, bottling, sterilization, the bottling amount is volumetrical 6/10-8/10, aseptic technique after 2-3 days, is blown static cultivation in the female kind access container of test tube (1) with air pump (12), about 25 ℃, cultivated 5-7 days, draw the liquid spawn injection large container of container (1) under the aseptic condition with syringe, inoculum size 10% is carried out and the same cultivation of container (1).

In the utility model, it is the millipore filtration (10-5) of 0.8-0.5 micron that the first layer of double-deck millipore filtration (10-5) can be selected the aperture, towards air pump (12) direction; It is the millipore filtration (10-5) of 0.4-0.22 micron that the second layer can be selected the aperture, the millipore filtration (10-5) of preferred 0.3-0.22 micron.The two-layer millipore filtration (10-5) that also can select the aperture to be the 0.4-0.22 micron, the millipore filtration (10-5) of preferred 0.3-0.22 micron.

The utility model adopts double-deck millipore filtration (10-5), the various bacteriums of filtering, germ effectively, the various moulds such as green mold, aspergillus, wood that Edible Fungi grows are mould, Mucor, Neurospora to influencing especially, success ratio is more than 98%, and less investment, instant effect, about hundred yuan of complete machine costs.

Claims

1, strain cultivation device, substratum (4) is housed in the container (1), vessel port (2) is by stopper (3) sealing, stopper (3) vertically has three through holes, be equipped with inlet pipe (5), vapor pipe (6) and drain pipe (7) in the through hole respectively, it is characterized in that: inlet pipe (5) inner port feeds in the substratum (4), and the part of inlet pipe (5) outside container (1) is provided with valve (9), strainer (10) and air pump (12) successively; Vapor pipe (6) inner port is higher than substratum (4) liquid level, and the part of vapor pipe (6) outside container (1) is provided with valve (13) and strainer (14) successively; Drain pipe (7) inner port is inserted container (1) bottom, and the part of drain pipe (7) outside container (1) is provided with valve (15).

2, strain cultivation device according to claim 1 is characterized in that: between strainer (10) and the air pump (12), be provided with under meter (11).

3, strain cultivation device according to claim 1 and 2, it is characterized in that: (10) two intermediate plates of strainer (10-1) middle part is provided with ventilating pit (10-2), ventilating pit (10-2) communicates with pipe connecting (10-3), intermediate plate (10-1) is provided with connecting hole (10-4) all around, be provided with millipore filtration (10-5) in the intermediate plate (10-1), the periphery of millipore filtration (10-5) seals with diaphragm seal (10-6).

4, strain cultivation device according to claim 3 is characterized in that: millipore filtration (10-5) is for double-deck, and its aperture is respectively the 0.8-0.5 micron, the 0.3-0.22 micron.

5, strain cultivation device according to claim 3 is characterized in that: millipore filtration (10-5) is for double-deck, and its aperture is the 0.3-0.22 micron.

6, strain cultivation device according to claim 3 is characterized in that: between millipore filtration (10-5) and diaphragm seal (10-6), be provided with liner (10-7).

7, strain cultivation device according to claim 3, it is characterized in that: inlet pipe (5) is located at the central authorities of stopper (3), the port of inlet pipe (5) in container (1) connects and disperses ball (8), disperses ball (8) can place the central authorities of substratum (4), and disperseing ball (8) is spherical shell shape.