CN2107468U - Small liquid deep layer ventilation culture device - Google Patents
Small liquid deep layer ventilation culture device Download PDFInfo
- Publication number
- CN2107468U CN2107468U CN 91230776 CN91230776U CN2107468U CN 2107468 U CN2107468 U CN 2107468U CN 91230776 CN91230776 CN 91230776 CN 91230776 U CN91230776 U CN 91230776U CN 2107468 U CN2107468 U CN 2107468U
- Authority
- CN
- China
- Prior art keywords
- pipe
- soft rubber
- rubber ball
- air
- sampling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
Abstract
The utility model belongs to the improvement of a small liquid deep layer ventilation culture device used for microorganism fermentation. The utility model is characterized in that a sealing type fermenter is provided with an air inlet pipe, an air outlet pipe, an inoculated pipe and a sampling discharge pipe. The inlet end of the air inlet pipe and the outlet end of the air outlet pipe are respectively provided with an air filter. Pipelines separately enter into the fermenter through a tank neck. The outlet end of the air inlet pipe is connected with an air dispersing pipe. The inlet end of the sampling discharge pipe is connected with a sampling rubber pipe. The device which simple structure and low cost is suitable for the microorganism, particularly for the production of yeast single cell protein and nodule bacteria fertilizer.
Description
The improvement of the utility model microorganism belonging to genus fermentation unit.
The deep liquid culture apparatus that is used for microorganism at present is made up of a series of fermentor tanks, and its tank body is generally made bilayer, is furnished with the stirring breather simultaneously.This class fermentor tank only is furnished with strainer at the inlet pipe entrance end, and do not dispose at escape pipe, when outage suddenly, a jar interior cultured microorganism often causes whole fermentor tank microbiological contamination owing to go into the unfiltered bacterium air that contains because of suck-back like this, so that gives to produce and bring very big loss.Simultaneously this class fermentor tank complex structure, cost an arm and a leg, be difficult to be midget plant that especially township and village enterprises adopts.
The purpose of this utility model is to overcome the deficiency of prior art, designs a kind of miniature liquid deep layer culture apparatus that microbial fermentation is used that is suitable for simple in structure, low-cost.
The utility model is achieved in that
A kind of main body is columniform pressure vessel, its upper end is the eck of a wide-mouth doleiform, soft rubber ball is arranged on the eck, there is device for fastening that itself and eck are sealed, import respectively into from described soft rubber ball, vapor pipe, sampling discharge nozzle and inoculated tube, wherein air filter is housed respectively at the entrance end of described inlet pipe and the exit end of vapor pipe, soft rubber ball is stretched out and to its relative separately direction bending in the outer end of the above-mentioned pipeline except that inoculated tube, its inner vertical feeding in the fermentor tank, the air dispersion pipe of perforate wherein is housed at the exit end of inlet pipe, the sampling discharge nozzle entrance end be connected with the sampling rubber tubing.Described inoculated tube is positioned at described soft rubber ball one side and keeps positioned vertical.
Below in conjunction with accompanying drawing the utility model is further described:
Fig. 1 is the signal of the utility model fermentor tank one-piece construction.Among the figure: the 1st, short rubber tubing (other similar rubber tubing is marked with 1 ' respectively); The 2nd, the sampling discharge nozzle; The 3rd, inoculated tube; The 4th, vapor pipe; The 5th, inlet pipe; The 6th, cover clamp; The 7th, tighten up screw rod; The 8th, the air inlet air filter; The 9th, the exhaust air filter; The 10th, soft rubber ball; The 11st, tank body; The 12nd, the air dispersion pipe; The 13rd, the sampling rubber tubing.
Fig. 2 is an air filter structural representation in the utility model.Among the figure: the 20th, the air inlet mouth of pipe; The 15th, upper cover plate; The 16th, the stainless steel tube porthole; The 17th, pressing plate; The 18th, cylindrical shell; The 19th, filtering medium layer; The 14th, exhaust pipe mouth; The 21st, base plate.
By Fig. 1, a kind of device that is used for the microbial liquid submerged fermentation is to be columniform tank body 11 by a main body, and it has the neck of a wide-mouth ampuliform.This tank body is made by the individual layer stainless steel plate, the jar eck is equipped with the soft rubber ball 10 suitable with neck finish diameter, this soft rubber ball by the cover clamp on it 6 with tighten up screw rod 7 and nut is connected on the tank body 11, straight formula inoculated tube 3 is housed respectively on this soft rubber ball, curved formula air inlet and exhaust piper 5,4, be connected with an air filter 8 at described inlet pipe entrance end, also be furnished with air filter 9, its objective is in order to improve the aseptic result of fermentor tank at the exhaust outlet end.Inserting one at the exit end of inlet pipe 5 has the air dispersion pipe 12 that presets aperture on it, and the entrance end at sampling discharge nozzle 2 is connected with a rubber hose 13 simultaneously, and its opening part is done engrail and stopped up when avoiding discharging.
As seen from Figure 2, it is taper that the sterile air of being sent here by total filter directly enters air filter 8 these strainer inlet and outlet mouth of pipe (20,14) tops, the lower end of strainer and cover plate 21 welderings are one, and its upper cover plate 15 is for there to be internal thread to be connected with the tank body 18 that has the outside screw interface
Two ends in this strainer are by stainless steel platen 17(or 17 ') and by stainless steel tube 16(or 16 ') filtration channel formed, between two passages filtering medium layer is arranged.This air filter further filtering total filter is not filtered clean indivedual assorted bacterium, to guarantee to obtain clean sterile air.
Using method of the present utility model: the nutritional need according to cultured microorganism prepares substratum, feed intake by fermentor tank volumetrical 3/4ths, add defoamer, the top breather is installed again, tighten up soft rubber ball, use the spiral tongs with the rubber tubing clamping on inlet pipe 5 and the sampling discharge nozzle 2 then, to prevent that a jar interior substratum is escaped to lose in the sterilization process, other exposes the mouth of pipe, and cotton is also good with soft paper bag beyond the Great Wall earlier.Ready fermentor tank is inoculated after the cooling 121 ℃ of following high-temperature sterilizations 90 minutes, places then and carries out aerated culture under the culture temperature.
Compared with the prior art, the utility model is simple in structure, and cost is low, can satisfy the particularly needs of the liquid deep layer cultivation of saccharomycete, rhizobium etc. of microorganism
Claims (1)
1, a kind of deep liquid culture apparatus that is used for microbial fermentation, form by closed fermenter, inlet pipe 5 entrance ends of this jar have an air filter 8, it is characterized in that, the main body of this jar is cylindrical, its neck is the wide-mouth ampuliform, the jar eck is equipped with the soft rubber ball 10 suitable with neck finish diameter, this soft rubber ball is by the cover clamp 6 on it, tightening up screw rod 7 and nut is connected on the tank body, inoculated tube 3 is housed respectively on this soft rubber ball, advance, vapor pipe 5,4 and the sampling discharge nozzle 2, inlet pipe 5 feeds in the fermentor tank by soft rubber ball 10, its exit end is connected with the air dispersion of perforate pipe 12 on one, exit end at vapor pipe 4 is connected with another air filter 9, vapor pipe 4 feeds in the fermentor tank by soft rubber ball 10, the entrance end of sampling discharge nozzle 2 is connected with a sampling rubber tubing 13, inoculated tube 3 is positioned at into, between the vapor pipe and pass described soft rubber ball and feed in the fermentor tank, described air filter 8,9 is a cylindrical tube, the one end is screw thread connection alive, the other end is fixedly connected, advancing in tube, exit end is respectively equipped with two filtration channels, is provided with filtering medium layer between two passages.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91230776 CN2107468U (en) | 1991-12-31 | 1991-12-31 | Small liquid deep layer ventilation culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 91230776 CN2107468U (en) | 1991-12-31 | 1991-12-31 | Small liquid deep layer ventilation culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2107468U true CN2107468U (en) | 1992-06-17 |
Family
ID=4936066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 91230776 Granted CN2107468U (en) | 1991-12-31 | 1991-12-31 | Small liquid deep layer ventilation culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2107468U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047199C (en) * | 1994-03-18 | 1999-12-08 | 四川华府高新工业设备研究所 | Solid state method single cell protein tower type fermentation machine |
| CN103275869A (en) * | 2013-06-05 | 2013-09-04 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN103286489A (en) * | 2013-05-30 | 2013-09-11 | 四川东方能源科技股份有限公司 | Cooling shell for welding equipment |
| CN105087358A (en) * | 2015-09-10 | 2015-11-25 | 即墨市第一职业中等专业学校 | Airlift strain fermentation apparatus |
| CN105765052A (en) * | 2013-10-31 | 2016-07-13 | 哈美顿博纳图斯股份公司 | Cover for a cell-culture container |
| CN109396159A (en) * | 2018-11-08 | 2019-03-01 | 杭州电子科技大学 | Self-control liquefaction hydrolysed ferment tank and fermentation process for laboratory |
-
1991
- 1991-12-31 CN CN 91230776 patent/CN2107468U/en active Granted
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1047199C (en) * | 1994-03-18 | 1999-12-08 | 四川华府高新工业设备研究所 | Solid state method single cell protein tower type fermentation machine |
| CN103286489A (en) * | 2013-05-30 | 2013-09-11 | 四川东方能源科技股份有限公司 | Cooling shell for welding equipment |
| CN103275869A (en) * | 2013-06-05 | 2013-09-04 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN103275869B (en) * | 2013-06-05 | 2014-10-22 | 浙江大学 | Aerobic thermophilic bacteria enrichment and culture device and its application method |
| CN105765052A (en) * | 2013-10-31 | 2016-07-13 | 哈美顿博纳图斯股份公司 | Cover for a cell-culture container |
| CN105765052B (en) * | 2013-10-31 | 2018-05-25 | 哈美顿博纳图斯股份公司 | For the cap of cell culture container |
| CN105087358A (en) * | 2015-09-10 | 2015-11-25 | 即墨市第一职业中等专业学校 | Airlift strain fermentation apparatus |
| CN109396159A (en) * | 2018-11-08 | 2019-03-01 | 杭州电子科技大学 | Self-control liquefaction hydrolysed ferment tank and fermentation process for laboratory |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |