CN1277456C - Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode - Google Patents
Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode Download PDFInfo
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- CN1277456C CN1277456C CN 200410018344 CN200410018344A CN1277456C CN 1277456 C CN1277456 C CN 1277456C CN 200410018344 CN200410018344 CN 200410018344 CN 200410018344 A CN200410018344 A CN 200410018344A CN 1277456 C CN1277456 C CN 1277456C
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Abstract
The present invention relates to a method and device for producing and inoculating oxygenating type liquid strains. The present invention has the technical scheme that superior strains are respectively enlarged and cultivated into the liquid strains in at least two fermentation vessels which are orderly connected by a closed pipeline system, and clean oxygenation air is ceaselessly charged in by an external liquid separate loading device when the liquid strains are enlarged and cultivated. An oxygenation device has the structure that air inlet tubes and connecting tubes are respectively connected to each sealing cover body of each fermentation vessel, wherein the air inlet tubes are connected with the liquid separate loading device, the connecting tubes are connected in series between the air outlet tubes and the fermentation vessels and are provided with valve switches, and the liquid separate loading device is connected with an oxygenation pump. The inoculation method quantificationally infiltrates the liquid strains into a culture medium used for culture by a liquid spray lance which is communicated with a pressure air source, and the liquid spray lance is communicated in a liquid strain fermentation tank by a material discharge tube. Compared with the prior art, the present invention has the characteristics of simple production methods, less equipment investment, high safety and reliability of the inoculation method, and high speed of inoculation; furthermore, the present invention omits the disinfection and sterilization time to an inoculation room and an inoculation box, is convenient for popularization and application, etc.
Description
Technical field
What the present invention relates to is production and inoculation method and their corresponding device thereof of a kind of edible fungi liquid strain, belongs to edible fungi liquid strain cultural method and device.
Background technology
The production technology of existing edible fungi liquid strain comprises: with excellent species (strain) enlarged culture successively is first class inoculum, secondary, three-class strain, and the equipment that common second class inoculum and three-class strain are adopted can be test tube, also can be fermentation tank etc.The liquid spawn that enlarged culture is become pours into made cultivation and cultivates with the inoculation of carrying out edible mushroom in the medium, and the liquid spawn of this edible mushroom is particularly suitable for the raw material bed and carries open inoculation.On the Chinese patent communique, disclose the patent of invention technology of " a kind of liquid culture method of edible fungi ", the patent No. is ZL86102421, the stingy amount deep ventilation that this patented technology adopts multi-stage sealed pipe-line system and disinfecting silk or cotton bouquet to filter stirs, get rid of shaking table, substituted air compressor etc.By the closed conduit system, utilize manifold pressure that the liquid spawn in the fermentation tank of previous stage is directly moved in the next stage fermentation tank.The liquid spawn production of prior art is used the purpose of fermentation tank or shaking table to be to increase and is needed certain oxygen in the microorganism mycelial growth, but this cover is mainly not only invested greatly by the liquid spawn production equipment that shaking table or fermentation tank are formed, and need the technical staff to possess certain know-how and technical merit, operation easier is big, and difficulty is applied in vast rural area.In addition, the liquid-spawn inoculation of enlarged culture is gone in the medium in the cultivation bag, still need operate having under the aseptic condition of facility, and lack accurately that inoculation quantitatively can not reach standardized production, inoculation cultivation efficient is low.
Summary of the invention
The objective of the invention is to overcome the deficiency of above-mentioned existence, and provide a kind of oxygenation generator that utilizes to be exclusively used in liquid spawn production and inoculation method and device.The production method of this oxygenation formula liquid spawn, it be with excellent species at least two rounds that link successively by the closed conduit system respectively enlarged culture become liquid spawn, ceaselessly charged into cleaning oxygenation air during the inherent enlarged culture liquid spawn of described round by the extraneous gas dispenser.
The oxygen-increasing device of liquid spawn production usefulness of the present invention, it is to be connected with the air inlet pipe of joining with the gas dispenser respectively on the sealing lid of each round, and the tube connector that respectively has valve switch of connecting between escape pipe and the round, described gas dispenser is connected to the oxygen increasing pump of a band air cleaner.
Described oxygen increasing pump includes a compressed pneumatic pump and oxygen generator to be formed, and compressed pneumatic pump is connected with oxygen generator after through an air cleaner, and again through being connected with the gas dispenser behind the air cleaner.
The inoculation method of liquid spawn of the present invention, it is that the liquid spawn access of enlarged culture one-tenth is cultivated in the medium of usefulness, adopts a gas spray gun that liquid spawn is quantitatively squeezed in the medium of cultivation usefulness.
Described spray gun is communicated in pressure gas source, and its gun barrel is communicated in the fermentation tank of liquid spawn by a discharge nozzle.
Described pressure gas source is-air compressor that the gun barrel of spray gun is connected on the discharge nozzle and is connected to a valve switch.
The present invention compared with prior art, characteristics such as it is easy to have production method, and equipment investment is little, and inoculation method is safe and reliable, and inoculation speed is fast, needn't operate at desinfection chamber, has save sterilization and sterilization time to transfer room and inoculating hood, and is easy to utilize.
Description of drawings
Fig. 1 is existing shaking table formula liquid spawn flow sheet.
Fig. 2 is that existing fermentation tank deep layer is cultivated the production procedure schematic diagram.
Fig. 3 is the technological process of production of the present invention and apparatus structure schematic diagram.
Embodiment
The present invention will be described in detail below in conjunction with accompanying drawing.
Shown in Figure 1, existing shaking table formula liquid spawn production technology comprises: be the level liquid bacterial classification with good quality strain (strain) enlarged culture in test tube 1 earlier, moving in vial 2 it again, enlarged culture is a second-class liquid isolate, again it is moved in another vial 3 once more that enlarged culture is three grades of liquid spawns, cultivation is housed in then liquid spawn being poured into in the plastic bag container 4 of medium.Shown in Figure 2, existing fermentation tank deep layer is cultivated to produce and is comprised: be the level liquid bacterial classification with good quality strain (strain) enlarged culture in test tube 5 earlier, it is moved into fermentation tank 6 inherent enlarged culture is second-class liquid isolate, again this second-class liquid isolate is moved in another fermentation tank 7 once more that enlarged culture is three grades of liquid spawns, cultivation is housed in three grades of liquid spawns are poured into in the plastic bag container 8 of medium.Aforesaid Chinese patent ZL86102421 patent of invention technology belongs to a kind of improvement technical solution of the fermentation tank deep layer cultivation production technology of above-mentioned structure shown in Figure 2.
The production method of oxygenation formula liquid spawn of the present invention, it be with excellent species at least two rounds that link successively by the closed conduit system respectively enlarged culture become liquid spawn, ceaselessly charged into cleaning oxygenation air during the inherent enlarged culture liquid spawn of described round by the extraneous gas dispenser.Shown in Figure 3, the oxygen-increasing device of liquid spawn production usefulness of the present invention, include three rounds 9 that link successively by the closed conduit system, on the sealing lid 10 of each round, be connected with the air inlet pipe 12 of joining respectively with gas dispenser 11, and the tube connector that respectively has valve switch 14 15 of connecting between escape pipe 13 and the round, described gas dispenser 11 is connected to the oxygen increasing pump 17 of a band air cleaner 16.Described oxygen increasing pump 17 includes a compressed pneumatic pump 18 and oxygen generator 19 to be formed, and compressed pneumatic pump 18 is connected with oxygen generator 19 after through an air cleaner 16A, and again through being connected with gas dispenser 11 behind the air cleaner 16B.The present invention is beneficial to the mycelial growth of microorganism in the round by the oxygen content of said apparatus to the increase of the liquid spawn in the round 9 air feed.
The present invention on the basis of the above, further improved the inoculation method of liquid spawn, shown in Figure 3, it is that the liquid spawn that enlarged culture becomes is inserted in the medium of cultivation usefulness, adopts a gas spray gun 20 that liquid spawn is quantitatively squeezed in the medium in the cultivation bag 21.Described spray gun 20 is communicated in pressure gas source 22, and its gun barrel is communicated in the round 9 of liquid spawn by a discharge nozzle 23.Described pressure gas source is an air compressor, and the gun barrel of spray gun 20 is connected on the discharge nozzle 23 and is connected to a valve switch.
Claims (2)
1, a kind of oxygen-increasing device that is used for oxygenation formula liquid spawn production method, it is characterized in that it is to be connected with the air inlet pipe (12) of joining with gas dispenser (11) on the sealing lid of each round (9) respectively, and the tube connector that respectively has valve switch (14) (15) of series connection between escape pipe (13) and the round (9), described gas dispenser (11) is connected to the oxygen increasing pump (17) of a band air cleaner (16A).
2, the oxygen-increasing device of oxygenation formula liquid spawn production method according to claim 1, it is characterized in that described oxygen increasing pump (17) includes a compressed pneumatic pump (18) and oxygen generator (19), and compressed pneumatic pump (18) is connected with oxygen generator (19) through an air cleaner (16A) back, and is connected with gas dispenser (11) through an air cleaner (16B) back again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410018344 CN1277456C (en) | 2004-05-14 | 2004-05-14 | Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410018344 CN1277456C (en) | 2004-05-14 | 2004-05-14 | Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1579124A CN1579124A (en) | 2005-02-16 |
| CN1277456C true CN1277456C (en) | 2006-10-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410018344 Expired - Fee Related CN1277456C (en) | 2004-05-14 | 2004-05-14 | Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode |
Country Status (1)
| Country | Link |
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| CN (1) | CN1277456C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100428877C (en) * | 2005-10-25 | 2008-10-29 | 上海丰科生物科技股份有限公司 | Edible mushroom collection method |
| CN103881910A (en) * | 2014-02-20 | 2014-06-25 | 北京科兴中维生物技术有限公司 | Aseptic operation pipeline system with external power output |
| CN106416749B (en) * | 2016-08-31 | 2020-02-07 | 四川宝生药业发展有限公司 | Method for reducing operation pollution in liquid strain culture process |
| CN107603857B (en) * | 2017-10-25 | 2018-05-18 | 翔天农业开发集团股份有限公司 | Liquid spawn pure oxygen inputs culture apparatus and method |
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2004
- 2004-05-14 CN CN 200410018344 patent/CN1277456C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1579124A (en) | 2005-02-16 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20061004 Termination date: 20100514 |