CN101543634B - Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter - Google Patents
Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter Download PDFInfo
- Publication number
- CN101543634B CN101543634B CN 200910066930 CN200910066930A CN101543634B CN 101543634 B CN101543634 B CN 101543634B CN 200910066930 CN200910066930 CN 200910066930 CN 200910066930 A CN200910066930 A CN 200910066930A CN 101543634 B CN101543634 B CN 101543634B
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- CN
- China
- Prior art keywords
- fermentation tank
- air filtration
- fermentation
- sterilization device
- filtration sterilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
Abstract
The invention relates to a method for sterilizing an air bacterial filter for fermentation and a fermentation tank provided with the air bacterial filter. The method for sterilizing the air bacterial filter for the fermentation comprises that: the air bacterial filter is arranged in a space above the fermentation liquid in a fermentation tank or in an interlayer of the fermentation tank or in a connected hopper of a fermentation tank body, an air inlet valve outside the tank body is connected to the air bacterial filter through a pipe in the tank, and the air bacterial filter is sterilized when the fermentation tank is sterilized. The fermentation tank of the invention comprises the fermentation tank (1), the air bacterial filter (3), the pipe (4) and the valve (5), and is characterized in that the fermentation tank is provided with at least one air bacterial filter, the air bacterial filter is arranged in a cavity (7) above the fermentation liquid (2), the pipe is vertical to the tank wall of the fermentation tank, one end of the pipe is communicated with the valve outside the fermentation tank, and the other end of the pipe is communicated with the air bacterial filter of the fermentation tank. The method has the advantage of simple operation, reduces link, and thoroughly ensures the sterilization.
Description
Technical field
This reality invention relates to a kind of fermentation tank with the sterilizing methods of air filtration sterilization device and is equipped with the fermentation tank of air filtration sterilization device
Background technology
Liquid fermentation technology is widely used in the fields such as food, pharmacy, fungi cultivation.One of key of this technology is installation for fermenting, and a good installation for fermenting should guarantee the fermentation technology realization, guarantees again to ferment to carry out under aseptic condition.For guaranteeing the gnotobasis of fermentation, to sterilize to fermentation tank, air filtration sterilization device, liquid desludging device before the fermentation.The fermentation tank that generally uses now all is the air filtration sterilization device to be arranged fermentation tank is exterior without exception, be connected with fermentation tank by pipeline, valve, for guaranteeing that sterilization thoroughly, take many measures, but complicated pipeline connects, repeatedly the troublesome operation of controlled valve, a link goes wrong, just cause sterilization not thorough, the air that enters fermentation tank is polluted with miscellaneous bacteria, tunning is impure.Such tunning, being used for pharmacy, food can the serious harm health, and is used for fungi cultivation, will can not grow sporophore as strain, and therefore such tunning can not use and scrap, and causes the waste of very large manpower and materials.
Summary of the invention
The purpose of this invention is to provide a kind of the fermentation with the sterilizing methods of air filtration sterilization device and the fermentation tank that is equipped with the air filtration sterilization device of realization the method
The air filtration sterilization device that generally uses now is to place outside the fermentation tank, is connected with fermentation tank with valve by pipeline, and the improvement of sterilization measure does not all change this traditional structural models.And the present invention looks for another way, and places the air filtration sterilization device in the fermentation tank or in its interlayer.
The present invention is a kind of ferments with the sterilizing methods of air filtration sterilization device, it is characterized in that; The air filtration sterilization device is installed in the interlayer of the space above the fermentation liquid in the fermentation tank or fermentation tank or in the disjunctor storehouse of fermentation tank tank body, the outer inlet port valve door of tank body is connected on the air filtration sterilization device by the tank interior conduit, when fermentation tank is sterilized, also finish the sterilization of air filtration sterilization device simultaneously.
A kind of fermentation tank that is equipped with the air filtration sterilization device of the present invention, comprise fermentation tank, air filtration sterilization device, pipeline, valve, it is characterized in that described fermentation tank is equipped with at least one air filtration sterilization device, described air filtration sterilization device is installed in the cavity above the fermentation liquid, and described pipeline vertically places on the tank skin of fermentation tank, pipeline one end is communicated with fermentation tank valve outward, and the other end is communicated with the air filtration sterilization device of fermentation tank.
Described air filtration sterilization device can also be installed in the interlayer of fermentation tank outside, and described pipeline vertically places the interlayer outer wall, and the interior cavity of the pore of air filtration sterilization device and fermentation tank is communicated with.
Can put the air filtration sterilization device of one group (a plurality of) in parallel or series connection in the fermentation tank of the present invention, realize that air enters in the tank or tank in the discharge of gas.
Work process: fermentation tank of the present invention, before giving the sterilization of fermentation tank body, close the outer air inlet/outlet valve of tank body, in the tank body sterilization simultaneously, also realized air filtration sterilization device and continuous pipeline sterilization thereof.During fermentation, open valve, air is sterilized behind the air filtration sterilization device and is entered in the tank; When being equipped with respectively two air filtration sterilization devices in the fermentation tank, the waste gas that produces in the fermentation tank is discharged by the valve of the outer venthole of tank body that another air filtration sterilization device in the tank is communicated with.Venthole also is furnished with the air filtration sterilization device, and purpose is that venthole pours in down a chimney air and causes the tank internal contamination when preventing the interior negative pressure of tank.
Beneficial effect of the present invention: the present invention places the air filtration sterilization device in the fermentation tank or in the interlayer, when fermentation tank is sterilized, namely the air filtration sterilization device also sterilized, and air filtration sterilization device disinfecting action is become simply, and is reliable; Compare when being connected connection air filtration sterilization device with fermentation tank, reduce the pipeline, the valve that connect, make the simple in structure of air filtration system, easy operating.
Description of drawings
Fig. 1 is that the air filtration sterilization device is installed in the cavity inner structure schematic diagram above the fermentation liquid;
Fig. 2 is the interlayer inner structure schematic diagram that the air filtration sterilization device is installed in the fermentation tank outside.
Labelling among the figure: 1-fermentation tank, 2-fermentation liquid, 3-air filtration sterilization device, 4-pipeline, 5-valve, 6-pore, 7-cavity, 8-interlayer.
Specifically execute real mode
Embodiment 1: the Auricularia liquid fermentation sterilizing methods of air filtration sterilization device
Use air filtration sterilization device is installed on the fermentation tank above the fermentation liquid, the Auricularia liquid fermentation and culture of packing in tank liquid, and with valve closing, fermentation tank is sterilized, and keeps in the time of 123 ℃ 45 minutes, also finishes the sterilization of air filtration sterilization device simultaneously.Immediately cooling, when temperature dropped to 25 ℃, inoculation Auricularia strain was opened the valve ventilation, begins fermentation; Fermentation ends, valve-off were carried out in fermentation 72 hours.
Embodiment 3: referring to Fig. 2, embodiment 2 described fermentation tanks, its air filtration sterilization device are installed in the interlayer 8 of fermentation tank outside, and described pipeline is perpendicular to the interlayer outer wall, and the interior cavity of the pore 6 of air filtration sterilization device and fermentation tank is communicated with.
The used air filtration sterilization device of the embodiment of the invention is commercially available air filtration sterilization device.
Claims (3)
1. a fermentation is characterized in that with air filtration sterilization device sterilizing methods; The air filtration sterilization device is installed in the interlayer of the space above the fermentation liquid in the fermentation tank or fermentation tank or in the disjunctor storehouse of fermentation tank tank body, the outer inlet port valve door of tank body is connected on the air filtration sterilization device by the tank interior conduit, when fermentation tank is sterilized, also finish the sterilization of air filtration sterilization device simultaneously.
2. fermentation tank that is equipped with the air filtration sterilization device, comprise fermentation tank (1), air filtration sterilization device (3), pipeline (4), valve (5) is characterized in that: described fermentation tank is equipped with at least one air filtration sterilization device, described air filtration sterilization device is installed in the top cavity (7) of fermentation liquid (2), on the tank skin of described pipeline perpendicular to fermentation tank, pipeline one end is communicated with fermentation tank valve outward, and the other end is communicated with the air filtration sterilization device of fermentation tank.
3. fermentation tank according to claim 2, it is characterized in that: described air filtration sterilization device is installed in the interlayer (8) of fermentation tank outside, described pipeline is perpendicular to the interlayer outer wall, and the pore of air filtration sterilization device (6) is communicated with cavity in the fermentation tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910066930 CN101543634B (en) | 2009-05-11 | 2009-05-11 | Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910066930 CN101543634B (en) | 2009-05-11 | 2009-05-11 | Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101543634A CN101543634A (en) | 2009-09-30 |
| CN101543634B true CN101543634B (en) | 2013-01-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910066930 Ceased CN101543634B (en) | 2009-05-11 | 2009-05-11 | Method for sterilizing air bacteria filter for fermentation tank and fermentation tank provided with air bacteria filter |
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| Country | Link |
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| CN (1) | CN101543634B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571746B (en) * | 2013-07-10 | 2015-01-14 | 温州兆曜机械科技有限公司 | Improved automatic medicine supplementation process through aseptic operation box |
| CN106829000B (en) * | 2017-02-08 | 2018-03-16 | 广州无添加主义化妆品有限公司 | The equipment and its sterilization filling method of sterilization filling facial mask |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2432215Y (en) * | 2000-06-06 | 2001-05-30 | 山东省科学院中日友好生物技术研究中心 | Equipment for making rice wine |
| CN2579900Y (en) * | 2002-11-14 | 2003-10-15 | 锁现民 | Deep layer cultivation fermentation pot medicinal liquid sterilizing air inlet and outler device for edible mushroom |
| CN2885869Y (en) * | 2005-09-23 | 2007-04-04 | 唐杰 | Simplified fermentor for producing liquid spawn |
-
2009
- 2009-05-11 CN CN 200910066930 patent/CN101543634B/en not_active Ceased
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2432215Y (en) * | 2000-06-06 | 2001-05-30 | 山东省科学院中日友好生物技术研究中心 | Equipment for making rice wine |
| CN2579900Y (en) * | 2002-11-14 | 2003-10-15 | 锁现民 | Deep layer cultivation fermentation pot medicinal liquid sterilizing air inlet and outler device for edible mushroom |
| CN2885869Y (en) * | 2005-09-23 | 2007-04-04 | 唐杰 | Simplified fermentor for producing liquid spawn |
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| Publication number | Publication date |
|---|---|
| CN101543634A (en) | 2009-09-30 |
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Decision date of declaring invalidation: 20180321 Decision number of declaring invalidation: 34919 Granted publication date: 20130123 |
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