CN104911096B - A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique - Google Patents
A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique Download PDFInfo
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- CN104911096B CN104911096B CN201510313920.3A CN201510313920A CN104911096B CN 104911096 B CN104911096 B CN 104911096B CN 201510313920 A CN201510313920 A CN 201510313920A CN 104911096 B CN104911096 B CN 104911096B
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- tank body
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The present invention provides a kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment, including tank body and fermentation tank, charging aperture is provided with the top of tank body, charging lid is provided with charging aperture, it is additionally provided with the top of tank body on air cleaner, air cleaner and is provided with compressed air inlet pipe, discharge pipe is additionally provided with tank body, tank base is goed deep into discharge pipe one end, and the other end stretches out tank body；It is provided with fermentation tank at the top of agitator, fermentation tank and is provided with inoculation pipeline, inoculation pipeline upper wall is provided with sterilizing steam inlet tube, inoculation pipeline lower wall is provided with steam condensate (SC) outlet；Wherein, discharge pipe front end is provided with discharging opening, and discharging opening nozzle diameter is less than inoculation pipeline, and discharging opening can be docked with being inoculated with the mouth of pipe of pipeline.The present invention utilizes direct and fermentation tank the inoculation mouth docking of the equipment, one-level kind is connected in fermentation tank using pressure difference, and the external world is not in contact with, the probability of infection miscellaneous bacteria is reduced to almost nil in seeded process, fermentation process is normally carried out, production efficiency is largely increased.
Description
Technical field
The present invention relates to a kind of fermentation first class inoculum culture of edible fungus liquid and the equipment of aseptic inoculation, and use the equipment
The technique of inoculation, belongs to biological fermentation field.
Background technology
In existing edible fungus species liquid fermentation process, one-level kind is all to be cultivated with triangular flask on shaking table, then
Alcohol Flame ring is utilized in fermentation plant, the inoculation mouth of fermentation tank is opened, the one-level kind in triangular flask is poured into fermentation tank.At this
During individual, the sterility of inoculation mouth on the cleanliness factor of fermentation plant, fermentation tank, the cleanliness factor of triangular flask and operating personnel
On inoculation, whether cleaning-less bacteria infection suffers from vital influence to qualification, and often each factor above is in seeded process
In it is careless slightly fermentation tank will be caused to infect miscellaneous bacteria, can occur the disaster of tank switching when serious, whole production process be influenceed, to enterprise
Industry causes huge economic loss.
The content of the invention
The application abandons present triangular flask shaking table culture technique, and using a kind of equipment, one is carried out in biochemical cultivation case
Level plants culture, docks the inoculation mouth of the discharging opening of the equipment and fermentation tank after having cultivated, is first carried out with steam docking port
Sterilizing, using aseptic compressed air, fermentation tank is pressed into by the first class inoculum in the equipment.With this technique be inoculated with to ensure
The miscellaneous rate of inoculation sense of fermentation tank is less than 0.1%, is much higher than conventional vaccination ways.It is specific as follows：
A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment, including tank body and fermentation tank, tank body top
Portion, which is provided with charging aperture, charging aperture, is provided with charging lid, is additionally provided with the top of tank body on air cleaner, air cleaner
It is provided with compressed air inlet pipe, tank body and is additionally provided with discharge pipe, tank base is goed deep into discharge pipe one end, the other end is stretched
Go out tank body；
It is provided with fermentation tank at the top of agitator, fermentation tank and is provided with inoculation pipeline, inoculation pipeline upper wall is provided with sterilizing
Steam inlet tube, inoculation pipeline lower wall is provided with steam condensate (SC) outlet；
Wherein, discharge pipe front end is provided with discharging opening, and discharging opening nozzle diameter is less than inoculation pipeline, and discharging opening is with connecing
Planting the mouth of pipe of pipeline can dock.
Described tank body internal upper part is additionally provided with tank lifting ear, and tank body inner bottom part is additionally provided with magnetic stirring bar, tank body lower part
It is additionally provided with magnetic stirring apparatus.
It is provided with described compressed air inlet pipe on compressed air control valve, discharge pipe and is provided with discharging control
It is provided with valve, inoculation pipeline on inoculation pipeline control valve, sterilizing steam inlet tube and is provided with steam control valve, steam condensate (SC) goes out
Steam control condensation valve is provided with mouth pipe.
The technique that the present invention is inoculated with and cultivated using the said equipment, comprises the following steps：
A. culture medium is poured into tank body from charging aperture, all valves and charging lid closed on tank body, 121
At DEG C, sterilized 30min, and test tube kind is connect on sterile platform after natural cooling, whole tank body is put into biochemical culture after inoculation
On magnetic stirring apparatus in case, cultivated, obtain first class inoculum；
B. the discharging opening of tank body is docked on the inoculation pipeline of fermentation tank after cultivating well, steam control valve is opened, from steaming
Steam pipe road is passed through steam and sterilized, after the completion of sterilizing, opens steam control condensation valve, discharges cold from steam condensate (SC) outlet
But water, steam off control valve and steam control condensation valve, open compressed air control valve, discharging control valve, inoculated tube successively
Road control valve, is accessed the first class inoculum in tank body in fermentation tank using the pressure of aseptic compressed air.
One-level kind is connected to fermentation tank by the present invention using direct and fermentation tank the inoculation mouth docking of the equipment using pressure difference
In, and the external world is not in contact with, infected in seeded process miscellaneous bacteria probability be reduced to it is almost nil so that fermentation process can normally be entered
OK, production efficiency is largely increased.
Brief description of the drawings
Fig. 1 is the front view of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment, wherein, 1. tank bodies, 2.
Air cleaner, 3. compressed air inlet pipes, 4. compressed air control valves, 5. charging apertures, 6. charging lids, 7. discharge pipes,
8. discharging control valve, 9. discharging openings, 10. tank lifting ears, 11. magnetic stirring bars, 12. magnetic stirring apparatus, 13. steam control valves, 14.
Sterilize steam inlet tube, 15. steam control condensation valves, 16. steam condensate (SC) outlets, 17. inoculation pipelines, 18. inoculation pipeline controls
Valve processed, 19. fermentation tanks.
Fig. 2 is the top view of tank body 1, wherein, 1. tank bodies, 2. air cleaners, 3. compressed air inlet pipes, 4. compressions are empty
Gas control valve, 7. discharge pipes, 8. discharging control valves, 9. discharging openings, 10. tank lifting ears.
Embodiment
Embodiment 1
A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment, including tank body 1 and fermentation tank 19, tank body
1 top, which is provided with charging aperture 5, charging aperture 5, is provided with charging lid 6, and the top of tank body 1 is additionally provided with air cleaner 2, air
It is provided with filter 2 in compressed air inlet pipe 3, tank body 1 and is additionally provided with discharge pipe 7, tank body is goed deep into the one end of discharge pipe 7
1 bottom, the other end stretches out tank body 1；
Agitator is provided with fermentation tank 19, the top of fermentation tank 19 is provided with inoculation pipeline 17, and the inoculation upper wall of pipeline 17 is set
Sterilizing steam inlet tube 14 is equipped with, the inoculation lower wall of pipeline 17 is provided with steam condensate (SC) outlet 16；
Wherein, the front end of discharge pipe 7 is provided with discharging opening 9, and the nozzle diameter of discharging opening 9 is less than inoculation pipeline 17,
And discharging opening 9 can be docked with being inoculated with the mouth of pipe of pipeline 17.
The described internal upper part of tank body 1 is additionally provided with 1 bottom in tank lifting ear 10, tank body and is additionally provided with magnetic stirring bar 11, tank
The bottom of body 1 is additionally provided with magnetic stirring apparatus 12.
It is provided with compressed air inlet pipe 3 on compressed air control valve 4, discharge pipe 7 and is provided with discharging control valve 8,
It is provided with inoculation pipeline 17 on inoculation pipeline control valve 18, sterilizing steam inlet tube 14 and is provided with steam control valve 13, steam is cold
Steam control condensation valve 15 is provided with solidifying water outlet pipe 16.
As shown in figure 1, a kind of edible fungus liquid fermentation aseptic inoculation technique, in edible fungus liquid fermenting and producing, culture
During first class inoculum, the culture medium prepared is poured into from the charging aperture 5 of tank body 1, and magnetic stirring bar 11 is put into tank body 1, is covered
Lid 6 is fed, compressed air control valve 4 and discharging control valve 8 is closed, sterilizing cabinet is put into and carries out 121 DEG C of sterilizing, 30min.Cooling
Test tube kind is accessed on aseptic working platform afterwards, whole tank body 1 is put into biochemical cultivation case after inoculation（It is interior to be provided with socket）Magnetic
Cultivated on power agitator 12.After culture is good, takes on fermentation tank, discharging opening 9 is connected to fermentation tank feed pipe 17, open
Steam control valve 13, is passed through steam by jet chimney 14 to fermentation tank inoculation mouth and sterilizes, and opens condensation water control valve 15
Cooling water is discharged, condensation water control valve 5 and steam control valve 14 is then shut off, filtrated air import 4 is opened, discharging control is opened
Valve 8 processed, opens the inoculation pipeline control valve 18 of fermentation tank 19, accesses the one-level kind in tank 1 using the pressure of filtrated air and sends out
Fermentation tank 19.
Claims (3)
- The aseptic inoculation technique 1. a kind of edible fungus liquid ferments, it is characterised in that in edible fungus liquid fermenting and producing, culture one During level strain, from tank body（1）Charging aperture（5）Pour into the culture medium prepared, and by magnetic stirring bar（11）It is put into tank body （1）, cover charging lid（6）, close compressed air control valve（4）And discharging control valve（8）, it is put into sterilizing cabinet and is sterilized 121 DEG C, 30min accesses test tube kind, by whole tank body after inoculation on aseptic working platform after cooling（1）It is put into biochemical cultivation case Interior magnetic stirring apparatus（12）It is upper to be cultivated, after culture is good, take on fermentation tank, by discharging opening（9）It is connected to fermentation tank charging Pipeline（17）, open steam control valve（13）, jet chimney is passed through to fermentation tank inoculation mouth（14）It is passed through steam to be sterilized, beats Open condensation water control valve（15）Cooling water is discharged, is then shut off condensing water control valve（5）And steam control valve（14）, open nothing Bacterium air intlet（4）, open discharging control valve（8）, open fermentation tank（19）Inoculation pipeline control valve（18）, utilize sterile sky The pressure of gas is by tank（1）In one-level kind access fermentation tank（19）；Equipment used in the technique includes tank body（1）And fermentation tank（19）, it is characterised in that tank body（1）Top is provided with charging Mouthful（5）, charging aperture（5）On be provided with charging lid（6）, tank body（1）Top is additionally provided with air cleaner（2）, air filtration Device（2）On be provided with compressed air inlet pipe（3）, tank body（1）Inside it is additionally provided with discharge pipe（7）, discharge pipe（7）One end is deep Enter tank body（1）Bottom, the other end stretches out tank body（1）；Fermentation tank（19）Inside it is provided with agitator, fermentation tank（19）Top is provided with It is inoculated with pipeline（17）, it is inoculated with pipeline（17）Upper wall is provided with sterilizing steam inlet tube（14）, it is inoculated with pipeline（17）Lower wall is provided with steaming Vapour condensating water outlet tube（16）；Wherein, discharge pipe（7）Front end is provided with discharging opening（9）, discharging opening（9）Nozzle diameter is less than inoculation pipeline（17）, and Discharging opening（9）With inoculation pipeline（17）The mouth of pipe can dock.
- The aseptic inoculation technique 2. edible fungus liquid according to claim 1 ferments, it is characterised in that tank body（1）Internal upper part It is additionally provided with tank lifting ear（10）, in tank body（1）Bottom is additionally provided with magnetic stirring bar（11）, tank body（1）Bottom is additionally provided with magnetic Power agitator（12）.
- The aseptic inoculation technique 3. edible fungus liquid according to claim 1 ferments, it is characterised in that compressed air inlet pipe （3）On be provided with compressed air control valve（4）, discharge pipe（7）On be provided with discharging control valve（8）, it is inoculated with pipeline（17）On It is provided with inoculation pipeline control valve（18）, sterilize steam inlet tube（14）On be provided with steam control valve（13）, steam condensate (SC) goes out Mouth pipe（16）On be provided with steam control condensation valve（15）.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510313920.3A CN104911096B (en) | 2015-06-10 | 2015-06-10 | A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510313920.3A CN104911096B (en) | 2015-06-10 | 2015-06-10 | A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique |
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| Publication Number | Publication Date |
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| CN104911096A CN104911096A (en) | 2015-09-16 |
| CN104911096B true CN104911096B (en) | 2017-11-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510313920.3A Expired - Fee Related CN104911096B (en) | 2015-06-10 | 2015-06-10 | A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique |
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105505744A (en) * | 2016-01-07 | 2016-04-20 | 盐城市雨露农业科技有限公司 | Liquid strain seed tank and using method thereof |
| CN106222078A (en) * | 2016-08-03 | 2016-12-14 | 王卫华 | A kind of biological fermentation tank |
| CN106172753A (en) * | 2016-08-23 | 2016-12-07 | 山西维尔生物乳制品有限公司 | A kind of liquid fermentation agent classification inoculation apparatus and method |
| CN108949517A (en) * | 2017-05-19 | 2018-12-07 | 上海吉态来生物技术有限公司 | A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe |
| CN107502535A (en) * | 2017-09-01 | 2017-12-22 | 苏州顺泰元虫草生物科技有限公司 | A kind of high yield cordyceps sinensis culture systems |
| CN107988056A (en) * | 2017-11-24 | 2018-05-04 | 成都凯成轻工药业装备有限公司 | A kind of strain scene classification inoculation apparatus and method |
| CN108285862A (en) * | 2018-04-18 | 2018-07-17 | 中国农业科学院农业资源与农业区划研究所 | Microbial ferment device |
| CN110218638A (en) * | 2019-07-02 | 2019-09-10 | 大连富森智能科技有限公司 | A kind of spawn fermentation tank |
| CN110669659A (en) * | 2019-07-05 | 2020-01-10 | 青海华牛生物科技有限公司 | Matrimony vine ferment feed liquid inoculation jar |
| CN113229041B (en) * | 2021-06-16 | 2023-12-01 | 绵阳菌源博文菌业有限公司 | Edible fungus liquid fermentation tank convenient to sterilize |
| CN115678749A (en) * | 2022-10-17 | 2023-02-03 | 安琪酶制剂(宜昌)有限公司 | Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202830031U (en) * | 2012-09-29 | 2013-03-27 | 北京华都诗华生物制品有限公司 | Pollution-free sterilization system for air inflow and feed supplement of bioreactor |
| CN204072829U (en) * | 2014-09-04 | 2015-01-07 | 福建将乐禾生原生态菌业有限公司 | A kind of edible mushroom steam sterilizing system of boiler afterheat recycling |
| CN204151250U (en) * | 2014-09-11 | 2015-02-11 | 湖北正佳微生物工程股份有限公司 | A kind of feed supplement tank with steam sterilizer |
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