CN215799515U - Simple and convenient inoculation utensil without contamination of bacteria - Google Patents
Simple and convenient inoculation utensil without contamination of bacteria Download PDFInfo
- Publication number
- CN215799515U CN215799515U CN202121838770.5U CN202121838770U CN215799515U CN 215799515 U CN215799515 U CN 215799515U CN 202121838770 U CN202121838770 U CN 202121838770U CN 215799515 U CN215799515 U CN 215799515U
- Authority
- CN
- China
- Prior art keywords
- bottle
- inoculation
- pipe
- bacteria
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a simple and convenient inoculation device without contamination of bacteria, which comprises an eggplant bottle, an inoculation bottle, a liquid through pipe and a vent pipe, wherein the liquid through pipe is respectively communicated to the tops of the eggplant bottle and the inoculation bottle, two ends of the vent pipe are respectively inserted into the middles of the eggplant bottle and the inoculation bottle, and the liquid through pipe and the vent pipe are respectively provided with a first valve and a second valve. By adopting the technical scheme, the simple and convenient inoculation device without contamination of bacteria not only reduces the exposure risk and the contamination probability, but also saves time and labor, and the vent pipe can keep the air pressure between the eggplant bottle and the inoculation bottle consistent when the liquid is poured, so that the pouring operation can be smoothly carried out.
Description
Technical Field
The utility model relates to a simple and convenient inoculation device without contamination of bacteria, and belongs to the technical field of strain cultivation.
Background
In the inoculation process of strain cultivation, an inoculation bottle, an eggplant bottle and a fermentation tank are generally required. The inoculation bottle is usually made of stainless steel, a flange joint for connecting the inoculation bottle and the outside is wrapped by multiple layers of gauze before inoculation, then wrapped by paper and tied by a rope, and then placed in an autoclave for sterilization at 121 ℃ for more than 30 min. After the bacterial lawn is cultured by the eggplant bottle, sterile water is poured into the eggplant bottle in a sterile room, the bacterial lawn on the eggplant bottle is cleaned by the sterile water and is communicated to the sterilized inoculation bottle through a pipeline, and then the bacterial lawn is poured into the inoculation bottle. When the fermentation tank is inoculated, the inoculation bottle is connected to the inoculation port of the fermentation tank, the inside of the pipe connected with the flange part needs to be disinfected for more than 30min through steam, then the pressure of the fermentation tank is increased, the pressure in the inoculation bottle is increased along with the pressure, then the pipeline valve is closed, the pressure in the fermentation tank is reduced, the inoculation bottle is inverted, then the valve is opened, and liquid in the inoculation bottle enters the fermentation tank under the action of gravity and pressure due to the inversion of the inoculation bottle. The above-mentioned drawbacks and deficiencies of the prior art include: the inoculation method needs to pour sterile water into the eggplant bottle in a sterile room, and then pour the cleaned lawn into the inoculation bottle, so that the infection probability is increased, two-person operation is needed, and the inoculation method is relatively labor-consuming.
SUMMERY OF THE UTILITY MODEL
Therefore, an object of the present invention is to provide a simple and convenient inoculation device which does not contaminate bacteria, does not require a sterile room for pouring sterile water into an eggplant bottle, and can effectively reduce the contamination probability during pouring of water.
In order to achieve the above object, the simple and convenient inoculation device without contamination of bacteria according to the present invention comprises an eggplant bottle, an inoculation bottle, a liquid passing pipe and a vent pipe, wherein the liquid passing pipe is respectively communicated to the tops of the eggplant bottle and the inoculation bottle, two ends of the vent pipe are respectively inserted into the middles of the eggplant bottle and the inoculation bottle, and the liquid passing pipe and the vent pipe are respectively provided with a first valve and a second valve.
The top of inoculation bottle is provided with the connecting pipe, connecting pipe and liquid pipe connect respectively in two ports of a three way connection, another port of three way connection connects in the branch pipe be provided with third valve and fourth valve on branch pipe and the connecting pipe respectively.
The simple and convenient inoculation device without bacteria also comprises a fermentation tank, and an inoculation port of the fermentation tank can be connected to the branch pipe.
By adopting the technical scheme, the simple and convenient inoculation device without contamination of bacteria can combine the eggplant bottle for culturing the bacteria and the inoculation bottle for inoculation together through the liquid through pipe, 200ml of sterile water is filled in the inoculation bottle, after the bacteria are cultured, the first valve and the second valve are opened, the inoculation bottle is inverted, the sterile water in the inoculation bottle flows into the eggplant bottle, the eggplant bottle is shaken to clean bacterial lawn with water, the eggplant bottle is inverted after the bacteria are cleaned, and the bacteria are poured into the inoculation bottle.
Drawings
Fig. 1 is a schematic view of the connection structure of the eggplant bottle and the inoculation bottle of the utility model.
FIG. 2 is a schematic view showing the connection structure of the fermenter and the seed bottle according to the present invention.
Detailed Description
The utility model is described in further detail below with reference to the figures and the detailed description.
The simple and convenient inoculation device without contamination of bacteria comprises an eggplant bottle 1, an inoculation bottle 2, a liquid through pipe 3 and a vent pipe 4, wherein the liquid through pipe 3 is respectively communicated to the tops of the eggplant bottle 1 and the inoculation bottle 2, two ends of the vent pipe 4 are respectively inserted into the middles of the eggplant bottle 1 and the inoculation bottle 2, and the liquid through pipe 3 and the vent pipe 4 are respectively provided with a first valve 5 and a second valve 6.
The top of the inoculation bottle 2 is provided with a connecting pipe 7, the connecting pipe 7 and the liquid through pipe 3 are respectively connected with two ports of a three-way joint 8, the other port of the three-way joint 8 is connected with a branch pipe 9, and the branch pipe 9 and the connecting pipe 7 are respectively provided with a third valve 10 and a fourth valve 11.
The simple and convenient inoculation device without bacteria also comprises a fermentation tank 12, and an inoculation port of the fermentation tank 12 can be connected to the branch pipe 9.
By adopting the technical scheme, the simple and convenient inoculation device without contamination of bacteria can combine the eggplant bottle 1 for culturing the bacteria and the inoculation bottle 2 for inoculation together through the liquid through pipe 3, 200ml of sterile water is filled in the inoculation bottle 2, after the bacteria culture is finished, the first valve 5 and the second valve 6 are opened, the inoculation bottle 2 is inverted, the sterile water in the inoculation bottle 2 flows into the eggplant bottle 1, the eggplant bottle 1 is shaken to clean bacterial lawn with water, the eggplant bottle 1 is inverted after the cleaning is finished, the bacteria are poured into the inoculation bottle 2, the operation not only reduces the exposure risk and reduces the contamination probability, but also saves time and labor, the vent pipe 4 can keep the air pressure between the eggplant bottle 1 and the inoculation bottle 2 consistent when the liquid is poured, and the pouring operation can be smoothly carried out.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the utility model.
Claims (3)
1. A simple and convenient inoculation instrument without contamination of bacteria is characterized in that: the liquid-passing pipe is respectively communicated to the tops of the eggplant bottle and the inoculation bottle, two ends of the air pipe are respectively inserted into the middle parts of the eggplant bottle and the inoculation bottle, and the liquid-passing pipe and the air pipe are respectively provided with a first valve and a second valve.
2. The convenient, non-microbial inoculant device according to claim 1 wherein: the top of inoculation bottle is provided with the connecting pipe, connecting pipe and liquid pipe connect respectively in two ports of a three way connection, another port of three way connection connects in the branch pipe be provided with third valve and fourth valve on branch pipe and the connecting pipe respectively.
3. The convenient, sterile inoculating device as recited in claim 2 wherein: the simple and convenient inoculation device without bacteria also comprises a fermentation tank, and an inoculation port of the fermentation tank can be connected to the branch pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121838770.5U CN215799515U (en) | 2021-08-05 | 2021-08-05 | Simple and convenient inoculation utensil without contamination of bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121838770.5U CN215799515U (en) | 2021-08-05 | 2021-08-05 | Simple and convenient inoculation utensil without contamination of bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215799515U true CN215799515U (en) | 2022-02-11 |
Family
ID=80129137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121838770.5U Active CN215799515U (en) | 2021-08-05 | 2021-08-05 | Simple and convenient inoculation utensil without contamination of bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215799515U (en) |
-
2021
- 2021-08-05 CN CN202121838770.5U patent/CN215799515U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104911096B (en) | A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique | |
| KR100767569B1 (en) | Fermentor | |
| CN215799515U (en) | Simple and convenient inoculation utensil without contamination of bacteria | |
| CN108641926A (en) | A kind of microbial fermentation avoids the system and method for microbiological contamination when inoculation | |
| CN202369585U (en) | Anaerobic inoculation tank | |
| CN203855567U (en) | Visual strain culture expanding fermentation tank device | |
| CN203537926U (en) | Edible fungus liquid culture production and cultivation system | |
| CN109762723A (en) | A kind of device for photosynthetic bacteria culture | |
| CN212589313U (en) | Inoculation device of liquefied strain | |
| CN206887113U (en) | A kind of classification inoculation apparatus of liquid spawn fermentation tank | |
| CN103548577A (en) | System and method for producing and culturing liquid spawns of edible fungus | |
| CN201049944Y (en) | Edible mushroom liquid bacterial fermentation device | |
| CN216919245U (en) | Seed moving device | |
| CN111052987A (en) | Inoculation device and inoculation method for liquefied strains | |
| JPH052307B2 (en) | ||
| CN215975779U (en) | Filter sterilization device for supplementing inducer in glucosamine fermentation process | |
| CN215947290U (en) | Sterile inoculation device of fermentation tank | |
| CN219972032U (en) | Sewage treatment equipment based on microbial technology | |
| CN203498384U (en) | Fermentation, seed-transplantation and distribution device | |
| CN2619944Y (en) | Inoculating device for seed pot | |
| CN221071484U (en) | Liquid fermentation tank for traditional Chinese medicine ferment | |
| CN213037748U (en) | Simple esterified red yeast rice culture device | |
| CN203382755U (en) | Inoculation supplementary device of fermentation tank | |
| CN217459409U (en) | In-situ culture device for tubular bacterial cellulose hydrogel | |
| CN209702756U (en) | A kind of classification inoculation apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |