CN217459409U - In-situ culture device for tubular bacterial cellulose hydrogel - Google Patents
In-situ culture device for tubular bacterial cellulose hydrogel Download PDFInfo
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- CN217459409U CN217459409U CN202221267927.8U CN202221267927U CN217459409U CN 217459409 U CN217459409 U CN 217459409U CN 202221267927 U CN202221267927 U CN 202221267927U CN 217459409 U CN217459409 U CN 217459409U
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Abstract
The utility model relates to an in-situ culture device of tubular bacterial cellulose hydrogel, which belongs to the controllable culture field of the bacterial cellulose hydrogel. Comprises an oxygen channel, an oxygen permeation tube and an air pump; the air pump is connected with the first oxygen interface and is used for pumping oxygen into the oxygen channel; two ends of the oxygen permeation tube are respectively connected with a first gas path conversion valve and a second gas path conversion valve, the first gas path conversion valve and the second gas path conversion valve respectively penetrate through opposite side walls of the culture container, and the oxygen permeation tube is positioned in the culture container; the culture container is used for culturing microorganisms capable of producing bacterial cellulose; the oxygen permeation tube is used for permeating oxygen into the culture container so as to induce acetobacter xylinum in the culture container to secrete bacterial cellulose on the outer wall of the oxygen permeation tube to form tubular bacterial cellulose hydrogel.
Description
Technical Field
The utility model relates to a controllable cultivation field of bacterial cellulose aquogel, more specifically relates to a tubular bacterial cellulose aquogel's normal position culture apparatus.
Background
The bacterial cellulose hydrogel as a natural polymer material has good biocompatibility and degradability, and can be widely applied to the fields of biomedical instruments, cosmetics, tissue engineering scaffolds, wound dressings and the like. In the field of tissue engineering, the bacterial cellulose hydrogel is designed into a tubular shape, and can be well applied to vascular stents and artificial blood vessels. The traditional production of the bacterial cellulose hydrogel is carried out by large-scale fermentation culture of acetobacter xylinum, and a culture medium containing the acetobacter xylinum has higher oxygen content at a liquid-gas junction, so that the acetobacter xylinum is induced to secrete at the liquid-gas junction to form a layer of bacterial cellulose hydrogel film. Therefore, the tubular bacterial cellulose hydrogel is required to be prepared only by curling and bonding the film-shaped bacterial cellulose hydrogel, the preparation process is complex, the conditions are not easy to control, and the obtained tubular bacterial cellulose hydrogel is easy to tear at the bonding position, so that the using effect is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a can normal position cultivate feasible device of tubulose bacterial cellulose aquogel, it is main including the oxygen passageway, strain the fungus membrane, the gas circuit change-over valve, the oxygen permeation tube, the air pump, store up the oxygen bag, the oxygen permeation tube exists in the culture container of filling with the bacterial culture medium, the air pump is gone into the oxygen passageway with the oxygen pump of storing in the oxygen storage bag, oxygen flows in the oxygen permeation tube through the gas circuit change-over valve behind the filtration fungus membrane, oxygen can be through permeating to inducing acetobacter xylinum in the culture medium to secrete bacterial cellulose on the outer wall of oxygen permeation tube and form pipy bacterial cellulose aquogel. Thereby solving the technical problems that the preparation process of the tubular bacterial cellulose hydrogel is complex and the adhesive part is easy to tear in the prior art.
According to the purpose of the utility model, the in-situ culture device of the tubular bacterial cellulose hydrogel is provided, which comprises an oxygen channel, an oxygen permeation tube and an air pump; the air pump is connected with the first oxygen interface and is used for pumping oxygen into the oxygen channel; two ends of the oxygen permeation tube are respectively connected with a first air path conversion valve and a second air path conversion valve, the first air path conversion valve and the second air path conversion valve respectively penetrate through the opposite side walls of the culture container, and the oxygen permeation tube is positioned in the culture container; the culture container is used for culturing microorganisms capable of producing bacterial cellulose; the oxygen permeation tube is used for permeating oxygen into the culture container;
a first bacteria filtering membrane is arranged between the air pump and the first air path conversion valve, a second bacteria filtering membrane is arranged between the second air path conversion valve and the second oxygen interface, and the first bacteria filtering membrane and the second bacteria filtering membrane are both positioned on the cross section of the oxygen channel; the first bacteria filtering membrane is used for filtering bacteria, so that sterile oxygen enters the oxygen permeable pipe through the first gas path conversion valve; and the second bacteria filtering membrane is used for filtering and sterilizing the oxygen gas which is transmitted out from the second gas path conversion valve again.
Preferably, the oxygen permeable tube is a polypropylene membrane, a polytetrafluoroethylene membrane, a polysulfone membrane or a polysiloxane membrane.
Preferably, the first and second bacterial filtration membranes are each independently selected from a polyvinyl chloride membrane, a polyacrylonitrile membrane, a polysulfone membrane, a polyethersulfone membrane, a sulfonated polysulfone membrane, a polyarylsulfone membrane, a polyvinylidene fluoride membrane, or a polytetrafluoroethylene membrane.
Preferably, the number of the oxygen permeation tubes is at least two.
Preferably, the number of the oxygen permeation tubes is 4-8.
Preferably, the distance between the first filter bacterium membrane and the first air path switching valve is 1-5 cm.
Preferably, the second filter bacterial membrane is positioned at the distance of 1-5cm from the second air path switching valve.
Preferably, an oxygen storage bag is connected between the first oxygen interface and the second oxygen interface.
Preferably, an oxygen tank or an oxygen bottle is connected between the first oxygen interface and the second oxygen interface.
Generally, through the utility model above technical scheme who thinks compares with prior art, mainly possesses following technical advantage:
(1) the utility model discloses an in situ culture device of tubulose bacterial cellulose aquogel cultivates the environment and seals relatively, is difficult for the contamination, has guaranteed the sterile environment among the tubulose bacterial cellulose forming process.
(2) The oxygen permeation tube in the in-situ culture device of the tubular bacterial cellulose hydrogel can allow oxygen to permeate and prevent liquid from permeating; and can carry out the culture side by side according to the demand, increase the number of oxygen tube side by side and can improve production efficiency in a flexible way.
(3) The utility model discloses an in situ culture apparatus of tubulose bacterial cellulose aquogel can initiatively support, improves oxygen permeation tube and culture medium juncture oxygen content, improves the efficiency that acetobacter xylinum secretes bacterial cellulose, and the forming process is quick.
Drawings
FIG. 1 is a schematic structural diagram of a tubular bacterial cellulose hydrogel in-situ culture device.
FIG. 2 is a schematic diagram of the steps of the tubular bacterial cellulose hydrogel in-situ culture device.
FIG. 3 is a schematic diagram of the secretion of bacterial cellulose by Acetobacter xylinum.
FIG. 4 is a schematic structural view of the tubular bacterial cellulose hydrogel in-situ culture apparatus of the present invention.
The same reference numbers will be used throughout the drawings to refer to the same elements or structures, wherein: 1-oxygen channel, 2-first filter membrane, 3-first air channel changing valve, 4-oxygen permeable tube, 5-air pump, 6-oxygen storage bag, 7-second air channel changing valve, 8-culture container, 9-second filter membrane, 10-acetobacter xylinum, 11-bacterial cellulose, 12-first oxygen interface and 13-second oxygen interface.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Furthermore, the technical features mentioned in the embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
In order to solve the problem that traditional bacterial cellulose production mode can not produce high-quality tubulose bacterial cellulose nature, the utility model provides a device that can normal position cultivation tubulose bacterial cellulose aquogel mainly includes oxygen passageway 1, first strain fungus membrane 2, first air circuit change-over valve 3, oxygen permeation tube 4, air pump 5, oxygen storage bag 6, and oxygen permeation tube 4 exists in the culture container 8 of filling with the bacterial culture medium. The air pump 5 pumps oxygen stored in the oxygen storage bag 6 into the oxygen channel 1, the oxygen flows into the oxygen permeation tube 4 through the first air path conversion valve 3 after passing through the first bacterial filtering membrane 2, the oxygen can permeate into the culture medium through the oxygen permeation tube 4 to induce acetobacter xylinum to secrete bacterial cellulose on the outer wall of the oxygen permeation tube 4 to form tubular bacterial cellulose hydrogel, and redundant oxygen flowing out of the oxygen permeation tube 4 enters the oxygen storage bag 6 through the oxygen channel 1 again.
According to the utility model discloses, air pump 5 promotes oxygen and gets into oxygen passageway 1, and oxygen gets back to once more through the circulation of round in storing up oxygen bag 6, and air pump 5 provides power for the circulation of oxygen, and oxygen bag 6 provides storage space for the oxysome, simultaneously, stores oxygen in oxygen bag 6, also can guarantee the sufficiency that oxygen was supplied with.
According to the utility model discloses, there are first fungus membrane 2 and second fungus membrane 9 of straining respectively at the side of first gas circuit change-over valve 3 and second gas circuit change-over valve 7, and first fungus membrane 2 and the second of straining strain the bacterium in the fungus membrane 9 can filter oxygen, prevent that the bacterial contamination of gas circuit from entering into in the culture medium of acetobacter xylinum.
According to the utility model discloses, during gas circuit change-over valve can get into oxygen permeation tube 4 for many oxygen passages in grades with an oxygen passage, oxygen permeation tube 4's quantity corresponds with the oxygen body passageway quantity after hierarchical, improves hierarchical gas circuit and oxygen permeation tube 4's quantity, can improve the output of tubular bacterial cellulose aquogel in the period of once cultivating.
According to the utility model discloses, oxygen permeating tube 4 can let oxygen see through and get into culture container 8, and the induced acetobacter xylinum gathering forms tubulose bacterial cellulose around oxygen permeating tube 4 outer wall and secreting bacterial cellulose.
According to the utility model discloses, the splendid attire has the acetobacter xylinum culture medium in the culture vessel 8, for the growth of acetobacter xylinum and the secretion of bacterial cellulose nature provide nutrition, the active acetobacter xylinum of inoculation after the high temperature autoclaving treatment of culture medium. The culture vessel 8 contains a culture medium of acetobacter xylinum capable of submerging the oxygen permeation tube 4.
Example 1
In order to explain the structure of the device for in situ culturing tubular bacterial cellulose hydrogel in the present invention in detail, the present embodiment will be described with reference to fig. 1, the present invention provides a device for in situ culturing tubular bacterial cellulose hydrogel, which mainly comprises an oxygen channel 1, a first bacteria filtering membrane 2, a first air path change-over valve 3, an oxygen permeable tube 4, an air pump 5 and an oxygen storage bag 6, wherein the oxygen permeable tube 4 is present in a culture container 8 filled with a bacterial culture medium. In this embodiment, the air pump 5 pumps oxygen stored in the oxygen storage bag 6 into the oxygen channel 1, the oxygen flows into the oxygen permeation tube 4 through the first air path conversion valve 3 after passing through the first bacterial filter membrane 2, the oxygen can permeate into the culture medium through the oxygen permeation tube 4 to induce acetobacter xylinum to secrete bacterial cellulose on the outer wall of the oxygen permeation tube 4 to form tubular bacterial cellulose hydrogel, and redundant oxygen flowing out of the oxygen permeation tube 4 enters the oxygen storage bag 6 again through the oxygen channel 1. Fig. 4 is a schematic structural diagram of the tubular bacterial cellulose hydrogel in-situ culture device of the present invention, namely, the oxygen storage bag 6 is replaced by a first oxygen interface 12 and a second oxygen interface 13 for accessing an oxygen device.
Example 2
To further illustrate the use of the in-situ tubular bacterial cellulose hydrogel culture apparatus of the present invention, the present embodiment will be described with reference to fig. 2, wherein the use steps of the apparatus are as follows:
step 1: performing ultraviolet sterilization on the device, and performing high-temperature high-pressure sterilization on the acetobacter xylinum culture medium to prepare a batch of acetobacter xylinum seeds;
step 2: preparing a sterile environment, and ensuring that the step 3 is carried out in the sterile environment;
and step 3: the culture medium of acetobacter xylinum is poured into the culture container 4, and 10: 1, inoculating acetobacter xylinum seeds;
and 4, step 4: culturing the device at 30 ℃ for 3 days to wait for the formation of tubular bacterial cellulose hydrogel;
and 5: taking out the oxygen permeation tube 4 in the culture container 8 together with the acetobacter xylinum culture medium, peeling off the tubular bacterial cellulose hydrogel, sequentially soaking the tubular bacterial cellulose hydrogel in alcohol and boiling the tubular bacterial cellulose hydrogel to be white or transparent by using 1mol/L NaOH solution, and finally processing the tubular bacterial cellulose hydrogel to be neutral by using ultrapure water.
Example 3
In order to further clarify the utility model discloses tubular bacterial cellulose aquogel's formation, this embodiment will combine fig. 3 to explain, the utility model discloses oxygen permeable pipe 4 can let oxygen pierce through in the pipe wall gets into the acetobacter xylinum culture medium of 8 splendid attires of culture vessel, as shown in fig. 3, oxygen is when passing oxygen permeable pipe 4, can pass in oxygen permeable pipe 4's pipe wall gets into acetobacter xylinum culture medium, form high oxygen content environment around oxygen permeable pipe 4 outer wall, make acetobacter xylinum 10 migrate around the pipe wall, utilize oxygen and nutrient substance that contain in the culture medium to secrete bacterial cellulose 11 simultaneously, bacterial fiber 11 when oxygen permeable pipe 4 pipe wall is around secretes to certain extent, just formed tubular bacterial cellulose aquogel.
It will be understood by those skilled in the art that the foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. An in-situ culture device of tubular bacterial cellulose hydrogel is characterized by comprising an oxygen channel (1), an oxygen permeation tube (4) and an air pump (5); the air pump (5) is connected with the first oxygen interface (12), and the air pump (5) is used for pumping oxygen into the oxygen channel (1); two ends of the oxygen permeable pipe (4) are respectively connected with the first air path switching valve (3) and the second air path switching valve (7), the first air path switching valve (3) and the second air path switching valve (7) respectively penetrate through opposite side walls of the culture container (8), and the oxygen permeable pipe (4) is positioned in the culture container (8); the culture container (8) is used for culturing microorganisms capable of producing bacterial cellulose; the oxygen permeation tube (4) is used for permeating oxygen into the culture container (8);
a first bacteria filtering membrane (2) is arranged between the air pump (5) and the first air path conversion valve (3), a second bacteria filtering membrane (9) is arranged between the second air path conversion valve (7) and the second oxygen interface (13), and the first bacteria filtering membrane (2) and the second bacteria filtering membrane (9) are both positioned on the cross section of the oxygen channel (1); the first bacteria filtering membrane (2) is used for filtering bacteria, so that sterile oxygen enters the oxygen permeable pipe (4) through the first gas path conversion valve (3); the second filter bacterial membrane (9) is used for filtering and sterilizing the oxygen permeating from the second gas path conversion valve (7) again.
2. The apparatus for in situ culturing of tubular bacterial cellulose hydrogel according to claim 1, wherein said oxygen permeable tube (4) is a polypropylene membrane, a polytetrafluoroethylene membrane, a polysulfone membrane or a polysiloxane membrane.
3. The apparatus for in situ culturing of tubular bacterial cellulose hydrogel according to claim 1, wherein said first (2) and second (9) filtration membranes are each independently selected from the group consisting of polyvinyl chloride, polyacrylonitrile, polysulfone, polyethersulfone, sulfonated polysulfone, polyarylsulfone, polyvinylidene fluoride and polytetrafluoroethylene membranes.
4. The apparatus for in situ culturing of tubular bacterial cellulose hydrogel according to claim 1, wherein the number of said oxygen permeable tubes (4) is at least two.
5. The in-situ culture device of the tubular bacterial cellulose hydrogel according to claim 4, wherein the oxygen permeable tubes (4) have 4-8 roots.
6. The apparatus for in situ culturing of tubular bacterial cellulose hydrogel according to claim 1, wherein the first filter membrane (2) is spaced from the first air passage switching valve (3) by 1-5 cm.
7. The in-situ culture device of the tubular bacterial cellulose hydrogel according to claim 1, wherein the second filter membrane (9) is located at a distance of 1-5cm from the second air passage switching valve (7).
8. The in-situ culture device of the tubular bacterial cellulose hydrogel according to claim 1, wherein an oxygen storage bag (6) is connected between the first oxygen interface (12) and the second oxygen interface (13).
9. The in-situ culture device of the tubular bacterial cellulose hydrogel according to claim 1, wherein an oxygen tank or an oxygen bottle is connected between the first oxygen interface (12) and the second oxygen interface (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221267927.8U CN217459409U (en) | 2022-05-24 | 2022-05-24 | In-situ culture device for tubular bacterial cellulose hydrogel |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221267927.8U CN217459409U (en) | 2022-05-24 | 2022-05-24 | In-situ culture device for tubular bacterial cellulose hydrogel |
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| CN217459409U true CN217459409U (en) | 2022-09-20 |
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| CN202221267927.8U Active CN217459409U (en) | 2022-05-24 | 2022-05-24 | In-situ culture device for tubular bacterial cellulose hydrogel |
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