CN107049800A - A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid - Google Patents
A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid Download PDFInfo
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- CN107049800A CN107049800A CN201710135977.8A CN201710135977A CN107049800A CN 107049800 A CN107049800 A CN 107049800A CN 201710135977 A CN201710135977 A CN 201710135977A CN 107049800 A CN107049800 A CN 107049800A
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- facial mask
- extraction liquid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, is obtained after the extract solution containing active components of plants using ultrasonic extraction, using gluconacetobacter is by screening, domestication and optimizes, obtains superior strain；Take the bacterial strain optimized to access in the culture medium after sterilizing, the culture medium after inoculation is uniformly poured into facial mask mould, after treatment, white clear shape nano-cellulose mask matrix；By face mask substrate material plastic sealing pack, autoclaving, obtain soaking after biology cellulose wet film in plant extraction liquid after sterilization and soak, vacuum drying 24h is carried out after freezing after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator, the nano-cellulose work(facial mask containing plant extraction liquid is made.The production technology of the present invention is easy to operate, pollution-free, and yield is high, greatly reduces production cost.
Description
Technical field
The present invention relates to the preparation technology of a kind of biological nano cellulose and natural plant series facial mask, it, which is mainly, passes through
Fermenting and producing, structure regulating, In-situ reaction angularly, develop biological nano cellulose and functionalization facial mask series of products.
Background technology
Biology cellulose facial mask is i.e. with medium of the biological fiber as facial mask.The fiber of Nano grade is opened from culture liquid level
Begin, in proper order from top to bottom towards incubator bottom it is careful weave, formed have Graded Density structure.The fiber of Nano grade
Far smaller than skin pore, can help Essence more effectively to import, and can more allow facial mask fully to be fitted skin.In addition, raw
The Graded Density structure of fibres vegetarian noodles film, can effectively adsorb the excess oil that skin is up to 92.7%, the grease in pore is led
Go out, while reaching the purpose of moisturizing and purification.In addition the also flexible good, toughness of biology cellulose facial mask is strong, non-stimulated be as good as
Taste, be difficult growth bacterium, without prominent stimulative substance, easily cleaning, the features such as no skin irritatin.Just because of biology cellulose facial mask
Advantageous property so that the product shows in cosmetic industry except huge prospect.
China Biofibre facial mask preparation technology relatively falls behind, and biology cellulose facial mask only has several on the market
Money, and most of is import, and facial mask is expensive（300 yuan/box of market average price, three, a box）So that vast consumption
Person is difficult to bear.
At present, acetobacter xylinum is used the strain for preparing biology cellulose facial mask, when acetobacter xylinum synthesizes fibre in cell more
After dimension element, wide about 1~2 nm parallel with the cell longitudinal axis sub- small fiber is secreted out of from the microchannel of bacteria cell wall, it is sub- small
Connected into and mutually wound between a diameter of 3~4 nm microfibre, microfibre by hydrogen bond between fiber, constitute mesh structural porous fibre
Silk ribbon is tieed up, its width is 40~100nm indefinite lengths, and crystal formation is cellulose.Filament band is interweaved, and forms mesh structural porous knot
Structure, and the gel film of the gas-liquid interface formation layer of transparent in culture medium.This tunic is biological cellulose membrane.Due to current
Biology cellulose facial mask is all fermented using acetobacter xylinum, and fermentation condition is more strict, causes manufacturing condition requirement high；It is raw
Fibres vegetarian noodles film is only obtained by same strain fermentation, may result in that mask product is single, and price is higher.In addition biological fiber
Vegetarian noodles film is because its nutritional ingredient is more, therefore is easier by bacterium infection.
The content of the invention
In order to develop new new Biofibre facial mask matrix, the price of Biofibre facial mask is reduced, China's fermentation is improved
Technology and the applicability for improving biotin facial mask.The present invention uses gluconacetobacter as fermented bacterium, while being trained to fermentation
Support parameter and Culture in situ is technically improved, obtain adding plant extraction liquid after ultra-thin facial mask in facial mask, improve product
Quality.
The process that the present invention is provided includes：A kind of preparation side of the nano-cellulose work(facial mask containing plant extraction liquid
Method, it is characterised in that the step is as follows：
（1）It is prepared by plant extraction liquid
After being dried naturally after natural plant active part is cleaned, the raw material powder that average grain diameter is 10~80 mesh is ground into
End, 1 is pressed by leaching liquor of 40% ~ 80% ethanol with plant powder:After 5 ~ 20 mass ratio mixing after 20 ~ 50min of ultrasonic time
It is transferred in pot for solvent extraction, 0.25 ~ 2 h is extracted under the conditions of 50 ~ 100 DEG C, extracting times is 2 times, are then filtered, filtrate warp
It is evaporated under reduced pressure and separates the solution after extract solution and obtain plant extraction liquid used in facial mask；
（2）The preparation of nano-cellulose mask matrix
The w/v% of hyaluronic acid 0.6 is added to the w/v% of glucose 4, the w/v% of peptone 0.6, the w/v% of yeast extract 0.4, citric acid
In 0.12 w/v%, disodium hydrogen phosphate 0.25w/v%, potassium dihydrogen phosphate 0.12w/v% culture medium, magnetic agitation 12h, control
Medium pH=6.5, and inoculation takes the bacterial strain activated after it is sterilized 35 minutes under 125 DEG C, 0.12 MPa, inoculum concentration is
12%, poured into after fully being vibrated during inoculation in facial mask mould, Silicon moulds sealing, 25 DEG C of constant temperature quiescent cultures are after 6 days, in ultra-pure water
In 100 DEG C boil 55min, remove remaining culture medium and cell debris, then with ultra-pure water cyclic washing or immersion until neutrality,
Obtain white clear shape nano-cellulose mask matrix；
（3）The preparation of nano-cellulose work(facial mask containing plant extraction liquid
By face mask substrate material plastic sealing pack, autoclaving obtains soaking plant extraction liquid after sterilization after biology cellulose wet film
Middle immersion, vacuum drying 24h is carried out after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator after freezing, be made and contained
The nano-cellulose work(facial mask of plant extraction liquid.
It is preferred that, step（1）Described in natural plant active part be hairyvein agrimony, whitewood's melon, one kind in white stilbene.
It is preferred that, step（1）Described in plant extraction liquid in ethanol content be less than 0.05%.
It is preferred that, step（2）Described in the bacterial strain that has activated be gluconacetobacter, its numbering is 1.1 812.
It is preferred that, step（3）Described in plant extraction liquid be hairyvein agrimony extract solution, whitewood's melon extract solution, white stilbene is extracted
At least one of liquid.
This technique productions obtains very thin layer of gel shape nano-cellulose facial mask substrate, and the substrate is ultra-thin 1/10 face
Film, the nano-cellulose film formed had both had the structure of " upper tightly lower sparse ", also has more than 100000 and micro- knits nano-pore, hole
Between be connected with each other by molecule hydrogen bond.This special structure and ultra micro nano aperture determine that the film has ultra-strong elasticity, pole
Strong ventilative and moisture holding capacity, the extremely strong performance for getting close to skin.The facial mask is combined with pure plant elite, can be directly rapid
Function Essence concentrated type is injected skin by ground, and multi-faceted maintenance skin is freely breathed by skin, allows skin to take on an entirely new look, and right
Skin does not have any side effect or ill effect.Its facial mask affordable, cost performance is high, definitely pure natural.
Also, by repeatedly screening, after experiment and summary, production process route of the invention is quite ripe.
Produce in preparation process, easy to operate, pollution-free, yield is high, greatly reduces production cost, there are good market prospects.
Embodiment
The present invention is described in further detail with reference to specific embodiment, but embodiments of the present invention are not limited to
This.
Embodiment one
A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that the step is as follows：
（1）It is prepared by plant extraction liquid
By hairyvein agrimony clean after dry naturally after, be ground into average grain diameter for 60 mesh material powder, using 55% ethanol as
Leaching liquor presses 1 with plant powder:It is transferred to after 10 mass ratio mixing after ultrasonic time 30min in pot for solvent extraction, in 55 DEG C of conditions
1 h of lower extraction, extracting times are 2 times, are then filtered, and solution of the filtrate after being evaporated under reduced pressure and separating extract solution obtains face
Hairyvein agrimony extract solution used in film；
（2）The preparation of nano-cellulose mask matrix
The w/v% of hyaluronic acid 0.6 is added to the w/v% of glucose 4, the w/v% of peptone 0.6, the w/v% of yeast extract 0.4, citric acid
In 0.12 w/v%, disodium hydrogen phosphate 0.25w/v%, potassium dihydrogen phosphate 0.12w/v% culture medium, magnetic agitation 12h, control
Medium pH=6.5, and inoculation takes the gluconacetobacter plant activate after it is sterilized 35 minutes under 125 DEG C, 0.12 MPa,
Inoculum concentration is 12%, is poured into after fully being vibrated during inoculation in facial mask mould, Silicon moulds sealing, and 25 DEG C of constant temperature quiescent cultures are after 6 days,
100 DEG C are boiled 55min in ultra-pure water, remove remaining culture medium and cell debris, then with ultra-pure water cyclic washing or immersion
Until it is neutral, obtain white clear shape nano-cellulose mask matrix；
（3）The preparation of nano-cellulose work(facial mask containing plant extraction liquid
By face mask substrate material plastic sealing pack, autoclaving, the hairyvein agrimony for obtaining soaking after biology cellulose wet film after sterilization is extracted
Soaked in liquid, vacuum drying 24h is carried out after freezing after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator, be made and contain
There is the nano-cellulose work(facial mask of plant extraction liquid.
Embodiment two
A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that the step is as follows：
（1）It is prepared by plant extraction liquid
By whitewood's melon clean after dry naturally after, be ground into average grain diameter for 45 mesh material powder, using 75% ethanol as
Leaching liquor presses 1 with plant powder:It is transferred to after 12 mass ratio mixing after ultrasonic time 40min in pot for solvent extraction, in 45 DEG C of conditions
1.5 h of lower extraction, extracting times are 2 times, are then filtered, and solution of the filtrate after being evaporated under reduced pressure and separating extract solution is obtained
Whitewood's melon extract solution used in facial mask；
（2）The preparation of nano-cellulose mask matrix
The w/v% of hyaluronic acid 0.6 is added to the w/v% of glucose 4, the w/v% of peptone 0.6, the w/v% of yeast extract 0.4, citric acid
In 0.12 w/v%, disodium hydrogen phosphate 0.25w/v%, potassium dihydrogen phosphate 0.12w/v% culture medium, magnetic agitation 12h, control
Medium pH=6.5, and inoculation takes the gluconacetobacter plant activate after it is sterilized 35 minutes under 125 DEG C, 0.12 MPa,
Inoculum concentration is 12%, is poured into after fully being vibrated during inoculation in facial mask mould, Silicon moulds sealing, and 25 DEG C of constant temperature quiescent cultures are after 6 days,
100 DEG C are boiled 55min in ultra-pure water, remove remaining culture medium and cell debris, then with ultra-pure water cyclic washing or immersion
Until it is neutral, obtain white clear shape nano-cellulose mask matrix；
（3）The preparation of nano-cellulose work(facial mask containing plant extraction liquid
By face mask substrate material plastic sealing pack, autoclaving, the whitewood's melon for obtaining soaking after biology cellulose wet film after sterilization is extracted
Soaked in liquid, vacuum drying 24h is carried out after freezing after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator, be made and contain
There is the nano-cellulose work(facial mask of plant extraction liquid.
Embodiment three
A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that the step is as follows：
（1）It is prepared by plant extraction liquid
After being dried naturally after white stilbene is cleaned, the material powder that average grain diameter is 40 mesh is ground into, using 65% ethanol as leaching
Extract presses 1 with plant powder:It is transferred to after 15 mass ratio mixing after ultrasonic time 50min in pot for solvent extraction, under the conditions of 65 DEG C
0.5h is extracted, extracting times are 2 times, are then filtered, solution of the filtrate after being evaporated under reduced pressure and separating extract solution obtains face
White stilbene extract solution used in film；
（2）The preparation of nano-cellulose mask matrix
The w/v% of hyaluronic acid 0.6 is added to the w/v% of glucose 4, the w/v% of peptone 0.6, the w/v% of yeast extract 0.4, citric acid
In 0.12 w/v%, disodium hydrogen phosphate 0.25w/v%, potassium dihydrogen phosphate 0.12w/v% culture medium, magnetic agitation 12h, control
Medium pH=6.5, and inoculation takes the gluconacetobacter plant activate after it is sterilized 35 minutes under 125 DEG C, 0.12 MPa,
Inoculum concentration is 12%, is poured into after fully being vibrated during inoculation in facial mask mould, Silicon moulds sealing, and 25 DEG C of constant temperature quiescent cultures are after 6 days,
100 DEG C are boiled 55min in ultra-pure water, remove remaining culture medium and cell debris, then with ultra-pure water cyclic washing or immersion
Until it is neutral, obtain white clear shape nano-cellulose mask matrix；
（3）The preparation of nano-cellulose work(facial mask containing plant extraction liquid
By face mask substrate material plastic sealing pack, autoclaving obtains soaking white stilbene extract solution after sterilization after biology cellulose wet film
Middle immersion, vacuum drying 24h is carried out after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator after freezing, be made and contained
The nano-cellulose work(facial mask of plant extraction liquid.
The hyaluronic acid that embodiment 1-3 is prepared-biological fiber compounded wet film performance difference is as shown in table 1
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| DPPH is removed（%） | 91.88 | 91.77 | 74.15 |
| TYR enzyme is removed（%） | -15.04 | 14.29 | 9.82 |
For the ordinary skill in the art, simply the present invention is exemplarily described for specific embodiment,
The obvious present invention, which is implemented, to be not subject to the restrictions described above, and is entered as long as employing the inventive concept and technical scheme of the present invention
The improvement of capable various unsubstantialities, or it is not improved by the present invention design and technical scheme directly apply to other occasions
, within protection scope of the present invention.
Claims (5)
1. a kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that the step is as follows：
It is prepared by plant extraction liquid
After being dried naturally after natural plant active part is cleaned, the raw material powder that average grain diameter is 10~80 mesh is ground into
End, 1 is pressed by leaching liquor of 40% ~ 80% ethanol with plant powder:After 5 ~ 20 mass ratio mixing after 20 ~ 50min of ultrasonic time
It is transferred in pot for solvent extraction, 0.25 ~ 2 h is extracted under the conditions of 50 ~ 100 DEG C, extracting times is 2 times, are then filtered, filtrate warp
It is evaporated under reduced pressure and separates the solution after extract solution and obtain plant extraction liquid used in facial mask；
（2）The preparation of nano-cellulose mask matrix
The w/v% of hyaluronic acid 0.6 is added to the w/v% of glucose 4, the w/v% of peptone 0.6, the w/v% of yeast extract 0.4, citric acid
In 0.12 w/v%, disodium hydrogen phosphate 0.25w/v%, potassium dihydrogen phosphate 0.12w/v% culture medium, magnetic agitation 12h, control
Medium pH=6.5, and inoculation takes the bacterial strain activated after it is sterilized 35 minutes under 125 DEG C, 0.12 MPa, inoculum concentration is
12%, poured into after fully being vibrated during inoculation in facial mask mould, Silicon moulds sealing, 25 DEG C of constant temperature quiescent cultures are after 6 days, in ultra-pure water
In 100 DEG C boil 55min, remove remaining culture medium and cell debris, then with ultra-pure water cyclic washing or immersion until neutrality,
Obtain white clear shape nano-cellulose mask matrix；
（3）The preparation of nano-cellulose work(facial mask containing plant extraction liquid
By face mask substrate material plastic sealing pack, autoclaving obtains soaking plant extraction liquid after sterilization after biology cellulose wet film
Middle immersion, vacuum drying 24h is carried out after being directly placed into face mask substrate material after facial mask adsorption saturation in refrigerator after freezing, be made and contained
The nano-cellulose work(facial mask of plant extraction liquid.
2. a kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that step（1）Described in
Natural plant active part be hairyvein agrimony, whitewood's melon, one kind in white stilbene.
3. a kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that step（1）Described in
Plant extraction liquid in ethanol content be less than 0.05%.
4. a kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that state step（2）Middle institute
It is gluconacetobacter to state the bacterial strain activated, and its numbering is 1.1 812.
5. a kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid, it is characterised in that step（3）Described in
Plant extraction liquid be hairyvein agrimony extract solution, whitewood's melon extract solution, at least one of white stilbene extract solution.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412142A (en) * | 2017-09-15 | 2017-12-01 | 广东贝豪生物科技有限公司 | Bacteria cellulose anti-inflammatory antiallergy bee venom facial mask |
| CN107595649A (en) * | 2017-09-15 | 2018-01-19 | 广东贝豪生物科技有限公司 | The manufacture method of binchotan Biofibre facial mask |
| CN107625664A (en) * | 2017-09-15 | 2018-01-26 | 广东贝豪生物科技有限公司 | Bacteria cellulose is used to prepare new application of facial mask thickener and products thereof |
| CN108360285A (en) * | 2018-01-19 | 2018-08-03 | 华南理工大学 | The preparation method for adulterating the silk paper of Cellulose nanocrystal palpus and its application in facial mask |
| CN108517719A (en) * | 2018-03-28 | 2018-09-11 | 华南理工大学 | Ultra-thin face paper of a kind of high water conservation highly-flexible and the preparation method and application thereof |
| CN108635329A (en) * | 2018-07-28 | 2018-10-12 | 高昕文 | A kind of preparation method of biology cellulose facial mask |
| CN109288723A (en) * | 2018-11-29 | 2019-02-01 | 华南理工大学 | A kind of cosmetic composition and preparation method thereof containing nano-cellulose |
| CN109316359A (en) * | 2018-11-29 | 2019-02-12 | 华南理工大学 | A kind of high moisture saver mask base fabric and preparation method thereof containing nano-cellulose fiber |
| CN110960467A (en) * | 2019-12-30 | 2020-04-07 | 河南汇博医疗股份有限公司 | Sterile mask and preparation method thereof |
| CN111686045A (en) * | 2020-06-02 | 2020-09-22 | 北京致成生物医学科技有限公司 | Skin-tendering facial mask made of plant-derived bacterial cellulose and preparation method of facial mask |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412142A (en) * | 2017-09-15 | 2017-12-01 | 广东贝豪生物科技有限公司 | Bacteria cellulose anti-inflammatory antiallergy bee venom facial mask |
| CN107595649A (en) * | 2017-09-15 | 2018-01-19 | 广东贝豪生物科技有限公司 | The manufacture method of binchotan Biofibre facial mask |
| CN107625664A (en) * | 2017-09-15 | 2018-01-26 | 广东贝豪生物科技有限公司 | Bacteria cellulose is used to prepare new application of facial mask thickener and products thereof |
| CN108360285A (en) * | 2018-01-19 | 2018-08-03 | 华南理工大学 | The preparation method for adulterating the silk paper of Cellulose nanocrystal palpus and its application in facial mask |
| CN108517719A (en) * | 2018-03-28 | 2018-09-11 | 华南理工大学 | Ultra-thin face paper of a kind of high water conservation highly-flexible and the preparation method and application thereof |
| CN108635329A (en) * | 2018-07-28 | 2018-10-12 | 高昕文 | A kind of preparation method of biology cellulose facial mask |
| CN109288723A (en) * | 2018-11-29 | 2019-02-01 | 华南理工大学 | A kind of cosmetic composition and preparation method thereof containing nano-cellulose |
| CN109316359A (en) * | 2018-11-29 | 2019-02-12 | 华南理工大学 | A kind of high moisture saver mask base fabric and preparation method thereof containing nano-cellulose fiber |
| CN109316359B (en) * | 2018-11-29 | 2021-05-14 | 华南理工大学 | High-moisture-retention mask base cloth containing nano cellulose fibers and preparation method thereof |
| CN110960467A (en) * | 2019-12-30 | 2020-04-07 | 河南汇博医疗股份有限公司 | Sterile mask and preparation method thereof |
| CN111686045A (en) * | 2020-06-02 | 2020-09-22 | 北京致成生物医学科技有限公司 | Skin-tendering facial mask made of plant-derived bacterial cellulose and preparation method of facial mask |
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Application publication date: 20170818 |