CN102392062A - Method for preparing bacterial cellulose by using decayed fruits as raw materials - Google Patents
Method for preparing bacterial cellulose by using decayed fruits as raw materials Download PDFInfo
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- CN102392062A CN102392062A CN2011103694608A CN201110369460A CN102392062A CN 102392062 A CN102392062 A CN 102392062A CN 2011103694608 A CN2011103694608 A CN 2011103694608A CN 201110369460 A CN201110369460 A CN 201110369460A CN 102392062 A CN102392062 A CN 102392062A
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Abstract
The invention relates to a method for preparing bacterial cellulose by using decayed fruits as raw materials, which comprises the following steps: 1) squeezing the decayed fruits and collecting juice, preparing juice with 2.5-5wt% of total sugar content by pure water and then disinfecting, carrying out membrane filtration and degerming to obtain the treated juice; 2) putting bacterial cellulose in the treated juice to prepare bacterial strain, standing under the temperature of 20-30 DEG C and culturing, or dynamically culturing under the temperature of 20-30 DEG C and the rotating speed of 50-400rpm, culturing for 5-20 days to obtain the bacterial cellulose. The invention has the advantages of simple preparation method, cheap and easy raw material acquisition and low cost, and is suitable for large scale production; the prepared bacterial cellulose has advantages of high output and wide application prospect.
Description
Technical field
The invention belongs to the preparation field of bacteria cellulose, particularly a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose.
Background technology
Bacteria cellulose (Bacterial Cellulose is called for short BC) is a kind of natural high polymer that is produced by microbial fermentation.Its chemical constitution is identical with plant cellulose; But show unique physical chemistry and mechanical property; Comprise high-crystallinity, high retentiveness, superfine nano fibre network, high-tensile and Young's modulus etc.; Being widely used in a plurality of fields such as food, papermaking, medical material, acoustical vibration film at present, is a kind of type material that receives much concern both at home and abroad.
Still there are some problems in the scale operation of bacteria cellulose at present, and is high like material cost, yield poorly etc.The fermentation of current bacteria cellulose is synthetic mainly to be that to cooperate nitrogen sources such as yeast extract paste, peptone with glucides such as glucose, fructose be raw material, and its cost is higher and output is lower.To this, seeking cheap raw material is that substratum fermentative prepn bacteria cellulose is an effective way that reduces its production cost.
Summary of the invention
Technical problem to be solved by this invention provides a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose, and this method is simple, and low in raw material cost is easy to get, and cost is low, is fit to large-scale production; The bacteria cellulose output height that the present invention is prepared.
A kind of method of utilizing rot fruit for the feedstock production bacteria cellulose of the present invention comprises:
(3) with after the rot fruit squeezing, collect juice, use pure water that it is mixed with the fruit juice liquid that total sugar content is 2.5～5wt%, sterilization is passed through the membrane filtration degerming, the fruit juice liquid after must handling again then;
(4) insert bacteria cellulose in the fruit juice liquid after above-mentioned processing and produce bacterial strain, under 20～30 ℃ of temperature, leave standstill cultivation, perhaps dynamically cultivate down at 20～30 ℃, 50～400rpm rotating speed, warp made bacteria cellulose in 5～20 days.
Described fruit in the step (1) is the fruit that contains carbohydrate.
Fruit described in the step (1) is one or more in apple, pears, peach, watermelon, grape and the Kiwifruit.
Sterilization described in the step (1) is carried out at normal pressure or condition of high voltage for adopting the sterilization of hot digestion sterilising method.
Above-mentioned hot digestion sterilization is for adopting high-pressure steam sterilizing pan in 121 ℃ of following boilings 20 minutes.
Membrane filtering method described in the step (1) is meant and adopts the filter membrane of 0.22 micron pore size to carry out the filtration sterilization of fruit juice.
Contain the yeast extract of 0.1～1wt% and the Tryptones of 0.1～0.5wt% in the fruit juice liquid after the processing described in the step (2).
It is acetic acid Pseudomonas, glyconic acid genus acetobacter, glucose anhydride Pseudomonas, rhizobium, Sarcina, Rhodopseudomonas, pseudomonas cepacia, achromobacter, Alcaligenes, aerobacter, Azotobacter, Agrobacterium or campylobacter jejuni or tea fungus that bacteria cellulose described in the step (2) is produced bacterial strain.
Contain nutritive substances such as a large amount of carbohydrates, VITAMINs in the fruit ingredient.China's fruit aboundresources, output is high, but the storage cycle of most fruits is shorter.Every year is owing to the rot fruit quantity that a variety of causes produces is very big.Fruit has the ethanol material to generate in the process of rotting.Bibliographical information is arranged, in substratum, add ethanol, can improve the output of bacteria cellulose.To this, it is cheap raw material that the present invention proposes with the rot fruit, through simply squeeze the juice, sterilising treatment, the synthetic bacteria cellulose that is used to ferment reduces its production cost.
Adding under the condition of identical nitrogenous source, it is 2.2 times of the bacteria cellulose output that obtains of raw material that the prepared bacteria cellulose output of the present invention can reach with glucose; Under the condition of not adding nitrogenous source, it is 46.8 times of the bacteria cellulose output that obtains of raw material that the prepared bacteria cellulose output of the present invention can reach with glucose.
Beneficial effect:
(1) preparation method of the present invention is simple, and low in raw material cost is easy to get, and cost is low, is fit to large-scale production;
(2) the prepared bacteria cellulose output of the present invention is that the bacteria cellulose output that raw material obtains obviously improves with glucose, is a kind of preparation method with potential promotion prospect.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Septic apple after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content as 2.5wt%.Adopt high-pressure steam sterilizing pan that fruit juice liquid is carried out sterilising treatment, insert acetobacter xylinum then, under 30 ℃ of conditions, leave standstill and cultivated 5 days, get bacteria cellulose film.
Embodiment 2
Septic peach after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content as 5wt%.Adopt the filter membrane of 0.22 micron pore size that fruit juice liquid is carried out the degerming processing, insert acetobacter xylinum then, under 30 ℃ of conditions, leave standstill and cultivated 6 days, get bacteria cellulose film.
Embodiment 3
Septic watermelon after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content, add the yeast extract of 0.1wt% and the Tryptones of 0.1wt% simultaneously as 3wt%.Adopt high-pressure steam sterilizing pan that fruit juice liquid is carried out sterilising treatment, insert acetobacter xylinum then, under 30 ℃ of conditions, leave standstill and cultivated 6 days, get bacteria cellulose film.
Embodiment 4
Septic pears after squeezing, are collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content, add the yeast extract of 1wt% and the Tryptones of 0.5wt% simultaneously as 2.5wt%.Adopt high-pressure steam sterilizing pan that fruit juice liquid is carried out sterilising treatment, insert acetobacter xylinum then, under 20 ℃ of conditions, leave standstill and cultivated 10 days, get bacteria cellulose film.
Embodiment 5
Septic grape after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content, adopt the filter membrane of 0.22 micron pore size that fruit juice liquid is carried out the degerming processing, insert acetobacter xylinum then, under 25 ℃ of conditions, leave standstill and cultivated 6 days, get bacteria cellulose film as 5wt%.
Embodiment 6
Septic Kiwifruit after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content, adopt the filter membrane of 0.22 micron pore size that fruit juice liquid is carried out the degerming processing, insert acetobacter xylinum then, under 30 ℃ of conditions, leave standstill and cultivated 6 days, get bacteria cellulose film as 2.5wt%.
Embodiment 7
Septic apple after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content as 2.5wt%.Adopt high-pressure steam sterilizing pan that fruit juice liquid is carried out sterilising treatment, insert acetobacter xylinum then, under 25 ℃ of conditions, 100rpm condition, cultivated 7 days, get the bacteria cellulose bead.
Embodiment 8
Septic peach after squeezing, is collected juice.Use pure water to be mixed with the fruit juice liquid of total sugar content as 3wt%.Adopt high-pressure steam sterilizing pan that fruit juice liquid is carried out sterilising treatment, insert acetobacter xylinum then, under 20 ℃ of conditions, 100rpm condition, cultivated 15 days, get the bacteria cellulose bead.
Claims (8)
1. method of utilizing rot fruit for the feedstock production bacteria cellulose comprises:
(1) with after the rot fruit squeezing, collect juice, use pure water that it is mixed with the fruit juice liquid that total sugar content is 2.5～5wt%, sterilization is passed through the membrane filtration degerming, the fruit juice liquid after must handling again then;
(2) insert bacteria cellulose in the fruit juice liquid after above-mentioned processing and produce bacterial strain, under 20～30 ℃ of temperature, leave standstill cultivation, perhaps dynamically cultivate down at 20～30 ℃, 50～400rpm rotating speed, warp made bacteria cellulose in 5～20 days.
2. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1, it is characterized in that: the described fruit in the step (1) is the fruit that contains carbohydrate.
3. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1 is characterized in that: the fruit described in the step (1) is one or more in apple, pears, peach, watermelon, grape and the Kiwifruit.
4. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1 is characterized in that: the sterilization described in the step (1) is for adopting the sterilization of hot digestion sterilising method under normal pressure or condition of high voltage.
5. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 4 is characterized in that: described hot digestion sterilization is for adopting high-pressure steam sterilizing pan in 121 ℃ of following boilings 20 minutes.
6. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1 is characterized in that: the membrane filtering method described in the step (1) is meant and adopts the filter membrane of 0.22 micron pore size to carry out the filtration sterilization of fruit juice.
7. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1 is characterized in that: contain the yeast extract of 0.1～1wt% and the Tryptones of 0.1～0.5wt% in the fruit juice liquid after the processing described in the step (2).
8. a kind of method of utilizing rot fruit for the feedstock production bacteria cellulose according to claim 1 is characterized in that: it is acetic acid Pseudomonas, glyconic acid genus acetobacter, glucose anhydride Pseudomonas, rhizobium, Sarcina, Rhodopseudomonas, pseudomonas cepacia, achromobacter, Alcaligenes, aerobacter, Azotobacter, Agrobacterium or campylobacter jejuni or tea fungus that the bacteria cellulose described in the step (2) is produced bacterial strain.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011103694608A CN102392062A (en) | 2011-11-18 | 2011-11-18 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
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| CN2011103694608A CN102392062A (en) | 2011-11-18 | 2011-11-18 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789370A (en) * | 2014-01-23 | 2014-05-14 | 中原工学院 | Method for processing bacterial cellulose cloth cutting pieces with fruit juice culture media |
| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN105166746A (en) * | 2015-08-30 | 2015-12-23 | 陈爱梅 | Preparation method of nutrient eleutherococcus sessiliflorus fruits |
| CN106983154A (en) * | 2017-04-05 | 2017-07-28 | 天津科技大学 | A kind of preparation method of Natta/chitosan dietary fiber |
| CN111264877A (en) * | 2020-02-04 | 2020-06-12 | 天津科技大学 | Preparation method of medicinal and edible high-fiber functional food |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1840677A (en) * | 2006-01-24 | 2006-10-04 | 李胜 | Method for preparing bacteria cellulose |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
| CN101985642A (en) * | 2010-12-09 | 2011-03-16 | 东华大学 | Method for preparing bacterial cellulose by using straw |
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2011
- 2011-11-18 CN CN2011103694608A patent/CN102392062A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840677A (en) * | 2006-01-24 | 2006-10-04 | 李胜 | Method for preparing bacteria cellulose |
| CN101805765A (en) * | 2010-04-01 | 2010-08-18 | 东华大学 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
| CN101985642A (en) * | 2010-12-09 | 2011-03-16 | 东华大学 | Method for preparing bacterial cellulose by using straw |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789370A (en) * | 2014-01-23 | 2014-05-14 | 中原工学院 | Method for processing bacterial cellulose cloth cutting pieces with fruit juice culture media |
| CN103789370B (en) * | 2014-01-23 | 2016-04-27 | 中原工学院 | The working method of a kind of fruit juice substratum bacteria cellulose cloth cut-parts |
| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN105166746A (en) * | 2015-08-30 | 2015-12-23 | 陈爱梅 | Preparation method of nutrient eleutherococcus sessiliflorus fruits |
| CN105166746B (en) * | 2015-08-30 | 2019-05-24 | 浙江维京生物科技有限公司 | A kind of preparation method of Sessileflower Acanthopanax Bark nutrition fruit |
| CN106983154A (en) * | 2017-04-05 | 2017-07-28 | 天津科技大学 | A kind of preparation method of Natta/chitosan dietary fiber |
| CN111264877A (en) * | 2020-02-04 | 2020-06-12 | 天津科技大学 | Preparation method of medicinal and edible high-fiber functional food |
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Application publication date: 20120328 |