CN101805765A - Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace - Google Patents
Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace Download PDFInfo
- Publication number
- CN101805765A CN101805765A CN201010137913A CN201010137913A CN101805765A CN 101805765 A CN101805765 A CN 101805765A CN 201010137913 A CN201010137913 A CN 201010137913A CN 201010137913 A CN201010137913 A CN 201010137913A CN 101805765 A CN101805765 A CN 101805765A
- Authority
- CN
- China
- Prior art keywords
- juice
- pomace
- fruit juice
- fruit
- bacterial cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace. The method comprises the following steps: (1) fruit juice culture solution is prepared, (2) pomace culture solution is prepared, (3) the bacterial cellulose is prepared, (4) the obtained bacterial cellulose is disacidified in drift ice, boiled in 10 to 30 wt percent of sweet water and then is dipped in the sweet water for 12 to 24h when the bacterial cellulose is still hot and the edible colorful bacterial cellulose can be obtained. Compared with the overseas synthesis culture base method, the utility model has the advantages of low cost and high yield. The invention can develop various edible colorful bacterial cellulose products with different colors, high color fastness, good taste and rich nutrition value.
Description
Technical field
The present invention relates to a kind of method of utilizing agricultural by-products to produce edible colorful bacterial cellulose, particularly relating to a kind of is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace.
Background technology
As a kind of emerging nano meter biomaterial, bacteria cellulose is subjected to the extensive concern of all circles day by day.Bacteria cellulose (also claiming micro organism cellulose) promptly is a kind of nanofiber by the biological process preparation, it is by wide about 10nm, the three-dimensional net structure that the nanofiber of thick about 3-8nm is formed, identical on its chemical constitution and the molecular structure with the natural plant fibre element, but there is not xylogen with the plant cellulose association, pectin and hemicellulose etc., have and to reach 95% high-crystallinity, the polymerization degree is up to 2000-8000, very strong water-holding power is arranged, higher biocompatibility is arranged, adaptability and good biodegradability are in medical treatment, food, papermaking, there is wide application prospect in fields such as acoustical vibration film.China still is in the starting stage in the research and development of bacteria cellulose this respect.Under world population growth and the situation that limited contradiction becomes increasingly conspicuous, world petroleum resource will be exhausted by exploitation in the several years from now on of ploughing, as a kind of purposes biomaterial very widely, bacteria cellulose is being contained infinite commercial opportunities and fine development prospect.Estimate that bacteria cellulose will develop into a big industry in China in the near future.
For a long time, synthetic required carbon source, the nitrogenous source of bacteria cellulose generally is materials such as glucose, fructose, peptone, yeast powder, and cost is higher, thereby has limited its production and application.The industrialization, the mass-producing that want to realize bacteria cellulose are used, and at first will reduce cost.In the past few decades, the production of bacteria cellulose and research have obtained bigger development, in Malaysia, Philippines, Brunei, Japan and other countries, utilize the by product Sucus Cocois of coconut processing to be raw material, under the effect of acetobacter xylinum, produce bacteria cellulose.Hainan Province is the main place of production of China's coconut, about 200,000,000 t of its annual production, and less than 2% of Gross World Product, and most of coconut quilt is directly edible or be processed to beverage, produces bacteria cellulose with it, is difficult to satisfy domestic growing consumers demand.Domestic have at present adopt peptone, extractum carnis, corn steep liquor etc. to produce bacteria celluloses, but the outward appearance of product and inner quality are all relatively poor, can't be used for commodity production at all.Therefore, press for the development of new carbon source and produce bacteria cellulose, provide foundation in the hope of production new way for China's bacteria cellulose.
China's bacteria cellulose is mainly used in food processing fields such as jelly, can, beverage at present simultaneously.Bacteria cellulose is as a kind of bacterial polysaccharides and food fibre, human body also had the function of many uniquenesses, as strengthening digestive function, preventing constipation, absorption and the effect of removing toxic substance in the food are arranged, also can optimize the environment in the Digestive tract simultaneously, play anti-aging effects.So, also more and more to it at present at the Application in Food Journal of Sex Research
[3]Simultaneously, the bacteria cellulose that we had is not only white on the market, also has the multiple bacteria cellulose of many colors, and mostly those colors are to add with artificial color makes, and colour fastness is low, and artificial color allows the people that doubt is arranged to the harm of HUMAN HEALTH.Therefore, the raw material that we want to utilize tool natural pigments such as abandoned fruit juice or pomace is as the cellulosic material of culturing bacterium, and inserting bacterial classification again carries out constant temperature culture, in the hope of creating riotous with colour coconut palm fruit, and natural stain replaces the prepared coconut palm fruit of artificial color, can be more healthy when edible and feel at ease.
The main component of fruit syrup is a moisture, in addition also contains a large amount of carbohydrates, protein and various trace element and amino acid, and these all are the synthetic requisite materials of bacteria cellulose.The fruit of Various Seasonal is in plentiful supply on the market, and is cheap, sometimes even large batch of breaking down, if can utilize its synthetic bacteria cellulose, will produce huge economic benefit.And all have every year big quality of lot not good or since some element and depleted fruit need to handle, and these raw materials are produced the good raw material of bacteria cellulose just, and not being used just becomes domestic refuse, and rotting, the smell is awful in the back, so that cause serious environmental pollution, become the environmental protection a great problem.
Summary of the invention
The purpose of this invention is to provide that a kind of cost is low, output is high, technology is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace simply, the present invention adopts abundant agricultural resource: orange, grape, Kiwifruit, apple, pears, pineapple, watermelon, strawberry, redly carry, mulberry juice and pomace etc., be processed into and be the main production substratum of bacteria cellulose, compare with the method for external employing synthetic medium, it is low to have cost, the advantage that output is high.And utilize the natural pigment that is contained in the various fruit juice, can develop the multiple abundant edible colorful bacterial cellulose product of different colours, colour fastness height, mouthfeel and nutritive value that has.A large amount of depleted fruit juice can be used, significant for environmental protection, simultaneously for the development that promotes plant husbandry such as various fruit, improve additional value of farm products, the significance of increasing farmers' income.Therefore, this preparation method not only has very considerable economic outlook, also will produce very remarkable social benefit.
Of the present invention is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, comprises following implementation step:
(1) preparation of fruit juice nutrient solution: clean silt and the impurity of discarding on the pericarp with flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit after perhaps will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity, obtains not having the juice of solid particulate; Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 3.5-5.5, juice is carried out sterilising treatment, low-temperature storage is standby then;
(2) preparation of pomace nutrient solution: the processing of the residue saccharification in pericarp and the fruit juice preparation process is obtained the saccharification treatment solution, use phosphate sodium dihydrogen buffer solution that saccharification treatment solution pH is adjusted to 3.5-5.5, the saccharification treatment solution is carried out sterilising treatment, and low-temperature storage is standby then;
(3) preparation of bacteria cellulose: choose above-mentioned prepared fruit juice nutrient solution, pomace nutrient solution or its mixture of a certain amount of dilution 2-10 after doubly as substratum; Or in this substratum, add in the inorganic salt of the nitrogenous source account for syrup that this substratum quality 5-15wt% concentration is 30-50wt%, 0.2-2wt% or 0.1-1wt% one or more, and once more sterilization as new substratum; To account for inoculum size inoculation acetobacter xylinum or yeast or the milk-acid bacteria of this substratum quality 0.1-2wt%, carry out constant temperature culture at 25-33 ℃, carry out the production of bacteria cellulose in 5-14 days;
(4) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 10-30wt% syrup and boils, and while hot it was soaked in the syrup 12-24 hour, promptly obtains edible colorful bacterial cellulose.
Preparation technology of the present invention compares with the method for external employing synthetic medium, and it is low to have a cost, the advantage that output is high, and gained colorful bacterial cellulose colour fastness height, edible safety health has different colours, mouthfeel and nutritive value, for the bacteria cellulose industrialization provides new way.
As optimized technical scheme:
Aforesaid is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, wherein, described fruit juice comprises orange juice, Sucus Vitis viniferae, Fructus actinidiae chinensis juice, Sucus Mali pumilae, pear juice, pineapple juice, Pulp Citrulli juice, strawberry juice, red extraction of the juice and morisuccus.
Aforesaid is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, wherein, described pomace comprises orange slag, trester, Kiwifruit slag, apple residue, pears slag, pineapple bran, watermelon slag, strawberry slag, red slag and the mulberries slag carried.
Aforesaid is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, and wherein, the temperature of described low-temperature storage is-5 ℃～-20 ℃.
Aforesaid is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, and wherein, described nitrogenous source is one or more in ammonium sulfate, peptone, yeast extract paste, soy peptone, the extractum carnis.
Aforesaid is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, and wherein, described inorganic salt are one or more in Trisodium Citrate, SODIUM PHOSPHATE, MONOBASIC, the sal epsom.
The invention has the beneficial effects as follows:
Of the present invention is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, and it is low to have a cost, easily the characteristics of industrialization.The agricultural resource abandoned fruit juice of employing China's abundant and pomace etc., be processed into and be the main production substratum of bacteria cellulose, compare with the method for external employing synthetic medium, it is low to have cost, the advantage that output is high, and gained colorful bacterial cellulose colour fastness height, edible safety health have different colours, mouthfeel and nutritive value, for the bacteria cellulose industrialization provides new way.The enforcement of this patent simultaneously can use a large amount of fruit juice of depleted in the processing of farm products, changed the situation of discarded fruit contaminate environment in the past, exploitation has the edible colorful bacterial cellulose product of low cost, low pollution, high added value, can bring huge economic and social benefit.
Embodiment
Below in conjunction with embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1:
(1) preparation of fruit juice nutrient solution: with silt and the impurity on the pericarp of the clean discarded orange of flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 4.5, juice is carried out sterilising treatment, ℃ store for future use then-5.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared orange juice nutrient solution of dilution after 2 times as substratum; With 0.1wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 7 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 10wt% syrup and boils, and while hot it was soaked in the syrup 24 hours, obtains edible orange orange flavor bacteria cellulose film.
Embodiment 2:
(1) preparation of fruit juice nutrient solution: the discarded apple fruit after will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity, obtains not having the juice of solid particulate; Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 3.5, juice is carried out sterilising treatment, ℃ store for future use then-20;
(2) preparation of bacteria cellulose: choose the above-mentioned prepared Sucus Mali pumilae nutrient solution after diluting 5 times, in this nutrient solution, add the yeast extract paste of 2wt% and the Trisodium Citrate of 0.1wt%, and sterilize once more as substratum; With 0.5wt% inoculum size inoculating lactic acid bacterium, carry out constant temperature culture at 32 ℃, carry out the production of bacteria cellulose in 14 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 15wt% syrup and boils, and while hot it was soaked in the syrup 24 hours, obtains the light yellow apple flavor of edible bacteria cellulose film.
Embodiment 3:
(1) preparation of pomace nutrient solution: the residue saccharification processing that will discard in orange pericarp and the fruit juice preparation process obtains the saccharification treatment solution, use phosphate sodium dihydrogen buffer solution that saccharification treatment solution pH is adjusted to 3.7, the saccharification treatment solution is carried out sterilising treatment, ℃ store for future use then-10;
(2) preparation of bacteria cellulose: choose the above-mentioned prepared orange slag nutrient solution after diluting 8 times, adding 5wt% concentration in this nutrient solution is the syrup of 30wt%, the ammonium sulfate of 0.6wt%, and sterilizes once more as substratum; With 0.8wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 12 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 10wt% syrup and boils, and while hot it was soaked in the syrup 20 hours, obtains edible orange orange flavor bacteria cellulose film.
Embodiment 4:
(1) preparation of fruit juice nutrient solution: with silt and the impurity on the pericarp of the clean discarded grape of flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 5.5, juice is carried out sterilising treatment, ℃ store for future use then-15.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared Sucus Vitis viniferae nutrient solution after diluting 4 times, adding 10wt% concentration in this nutrient solution is the syrup of 35wt%, the peptone of 0.6wt%, the Trisodium Citrate of 0.5wt%, and sterilizes once more as substratum; With 1.2wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 25 ℃, carry out the production of bacteria cellulose in 9 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 18wt% syrup and boils, and while hot it was soaked in the syrup 18 hours, obtains edible purple grape flavor bacteria cellulose film.
Embodiment 5:
(1) preparation of fruit juice nutrient solution: the discarded kiwifruit fruit after will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity, obtains not having the juice of solid particulate; Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 4.0, juice is carried out sterilising treatment, ℃ store for future use then-5;
(2) preparation of bacteria cellulose: choose the above-mentioned prepared Fructus actinidiae chinensis juice nutrient solution of dilution after 10 times as substratum; With 2wt% inoculum size inoculation yeast bacterium, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 6 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 10wt% syrup and boils, and while hot it was soaked in the syrup 12 hours, obtains the green Kiwifruit flavor of edible bacteria cellulose film.
Embodiment 6:
(1) preparation of fruit juice nutrient solution: with silt and the impurity on the pericarp of the clean discarded pears of flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 4.2, juice is carried out sterilising treatment, ℃ store for future use then-5.
(2) preparation of pomace nutrient solution: the residue saccharification processing that will discard in the operatic circle skin and the pear juice preparation process obtains the saccharification treatment solution, use phosphate sodium dihydrogen buffer solution that saccharification treatment solution pH is adjusted to 4.2, the saccharification treatment solution is carried out sterilising treatment, ℃ store for future use then-5;
(3) preparation of bacteria cellulose: pears slag nutrient solution mixture is as substratum after choosing the above-mentioned prepared pear juice nutrient solution after diluting 5 times and diluting 2 times; With 1.6wt% inoculum size inoculating lactic acid bacterium, carry out constant temperature culture at 27 ℃, carry out the production of bacteria cellulose in 5 days.
(4) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 16wt% syrup and boils, and while hot it was soaked in the syrup 20 hours, obtains edible oyster white pears flavor bacteria cellulose film.
Embodiment 7:
(1) preparation of fruit juice nutrient solution: the discarded pineapple fruit after will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity, obtains not having the juice of solid particulate; Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 5.5, juice is carried out sterilising treatment, ℃ store for future use then-8.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared pineapple juice nutrient solution of dilution after 10 times as substratum; With 0.9wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 29 ℃, carry out the production of bacteria cellulose in 10 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 18wt% syrup and boils, and while hot it was soaked in the syrup 19 hours, obtains the yellow pineapple flavor of edible bacteria cellulose film.
Embodiment 8:
(1) preparation of fruit juice nutrient solution: the discarded watermelon fruit after will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 4.1, juice is carried out sterilising treatment, ℃ store for future use then-5.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared Pulp Citrulli juice nutrient solution after diluting 7 times, adding 15wt% concentration in this nutrient solution is the syrup of 26wt%, the soy peptone of 0.2wt%, the sal epsom of 1wt%, and sterilizes once more as substratum; With 0.3wt% inoculum size inoculation yeast bacterium, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 7 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 16wt% syrup and boils, and while hot it was soaked in the syrup 15 hours, obtains edible light red watermelon flavor bacteria cellulose film.
Embodiment 9:
(1) preparation of fruit juice nutrient solution: with silt and the impurity on the clean abandoned strawberry of flowing water, with the hollander making beating, pulp wraps up squeezing juice with cloth; Orange juice after the coarse filtration filters, separates through centrifugal filter, removes remaining fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 3.5, juice is carried out sterilising treatment, ℃ store for future use then-12.
(2) preparation of pomace nutrient solution: the processing of the residue saccharification in the abandoned strawberry fruit juice preparation process is obtained the saccharification treatment solution, use phosphate sodium dihydrogen buffer solution that saccharification treatment solution pH is adjusted to 3.5, the saccharification treatment solution is carried out sterilising treatment, ℃ store for future use then-12;
(3) preparation of bacteria cellulose: strawberry slag nutrient solution mixture is as substratum after choosing the above-mentioned prepared strawberry juice nutrient solution after diluting 2 times and diluting 2 times; Adding 12wt% concentration in this substratum is the syrup of 30wt%, the peptone of 0.2wt%, the Trisodium Citrate of 0.8wt%, and sterilizes once more as new substratum; With 1.8wt% inoculum size inoculating lactic acid bacterium, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 14 days.
(4) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 30wt% syrup and boils, and while hot it was soaked in the syrup 12 hours, obtains edible bright red strawberry flavor bacteria cellulose film.
Embodiment 10:
(1) preparation of fruit juice nutrient solution: clean silt and the impurity of discarding on the red pericarp of carrying with flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 3.8, juice is carried out sterilising treatment, ℃ store for future use then-14.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared red extraction of the juice nutrient solution of dilution after 2 times as substratum; With 0.6wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 30 ℃, carry out the production of bacteria cellulose in 12 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 25wt% syrup and boils, and while hot it was soaked in the syrup 17 hours, obtains the red bacteria cellulose film that renders palatable of edible red-purple.
Embodiment 11:
(1) preparation of fruit juice nutrient solution: clean silt and the impurity of discarding mulberries with flowing water, with the hollander making beating, pulp wraps up squeezing juice with cloth; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining fruit seed, part fiber, broken fruit pulp and impurity.Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 5.0, juice is carried out sterilising treatment, ℃ store for future use then-5.
(2) preparation of bacteria cellulose: choose the above-mentioned prepared morisuccus nutrient solution after diluting 4 times, adding 16wt% concentration in this nutrient solution is the syrup of 50wt%, the extractum carnis of 0.6wt%, the SODIUM PHOSPHATE, MONOBASIC of 0.5wt%, and sterilizes once more as substratum; With 2wt% inoculum size inoculation acetobacter xylinum, carry out constant temperature culture at 33 ℃, carry out the production of bacteria cellulose in 14 days.
(3) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the 27wt% syrup and boils, and while hot it was soaked in the syrup 22 hours, obtains edible garnet mulberries flavor bacteria cellulose film.
Claims (7)
1. be the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that comprising following implementation step:
(1) preparation of fruit juice nutrient solution: clean silt and the impurity of discarding on the pericarp with flowing water, with hollander making beating peeling, pulp wraps up squeezing juice with cloth; Fruit after perhaps will cleaning is poured in the squeezing machine and is squeezed the juice, again through scraping panel filter elimination pericarp, fruit seed and part robust fibre; Fruit juice after the coarse filtration filters, separates through centrifugal filter, removes remaining pericarp, fruit seed, part fiber, broken fruit pulp and impurity, obtains not having the juice of solid particulate; Use phosphate sodium dihydrogen buffer solution that juice pH is adjusted to 3.5-5.5, juice is carried out sterilising treatment, low-temperature storage is standby then;
(2) preparation of pomace nutrient solution: the processing of the residue saccharification in pericarp and the fruit juice preparation process is obtained the saccharification treatment solution, use phosphate sodium dihydrogen buffer solution that saccharification treatment solution pH is adjusted to 3.5-5.5, the saccharification treatment solution is carried out sterilising treatment, and low-temperature storage is standby then;
(3) preparation of bacteria cellulose: choose above-mentioned prepared fruit juice nutrient solution, pomace nutrient solution or its mixture of a certain amount of dilution 2-10 after doubly as substratum; Inoculum size inoculation acetobacter xylinum or yeast or milk-acid bacteria with 0.1-2wt% carry out constant temperature culture at 25-33 ℃, carry out the production of bacteria cellulose in 5-14 days;
(4) the gained bacteria cellulose carries out the drift ice disacidify and handles, and is placed on then in the syrup that concentration is 10-30wt% and boils, and while hot it was soaked in the syrup 12-24 hour, promptly obtains edible colorful bacterial cellulose.
2. as claimed in claim 1 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that described fruit juice comprises orange juice, Sucus Vitis viniferae, Fructus actinidiae chinensis juice, Sucus Mali pumilae, pear juice, pineapple juice, Pulp Citrulli juice, strawberry juice, red extraction of the juice and morisuccus.
3. as claimed in claim 1 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that described pomace comprises orange slag, trester, Kiwifruit slag, apple residue, pears slag, pineapple bran, watermelon slag, strawberry slag, red slag and the mulberries slag carried.
4. as claimed in claim 1 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that, the temperature of described low-temperature storage is-5 ℃～-20 ℃.
5. as claimed in claim 1 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that, add in the described substratum that to account for this substratum quality 5-15wt% concentration be the syrup of 30-50wt% and/or account for the nitrogenous source of this substratum quality 0.2-2wt% and/or account for the inorganic salt of this substratum quality 0.1-1wt%, and once more sterilization as new substratum.
6. as claimed in claim 5 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that described nitrogenous source is one or more in ammonium sulfate, peptone, yeast extract paste, soy peptone, the extractum carnis.
7. as claimed in claim 5 is the method for raw material production edible colorful bacterial cellulose with abandoned fruit juice or pomace, it is characterized in that described inorganic salt are one or more in Trisodium Citrate, SODIUM PHOSPHATE, MONOBASIC, the sal epsom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010137913 CN101805765B (en) | 2010-04-01 | 2010-04-01 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010137913 CN101805765B (en) | 2010-04-01 | 2010-04-01 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101805765A true CN101805765A (en) | 2010-08-18 |
| CN101805765B CN101805765B (en) | 2013-10-30 |
Family
ID=42607718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010137913 Active CN101805765B (en) | 2010-04-01 | 2010-04-01 | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101805765B (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102911982A (en) * | 2012-11-23 | 2013-02-06 | 海南椰国食品有限公司 | Application of bananas in biocellulose fermentation media |
| CN102925514A (en) * | 2012-11-23 | 2013-02-13 | 海南椰国食品有限公司 | Application of mangos in bio-cellulose fermentation medium |
| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN104263624A (en) * | 2014-10-23 | 2015-01-07 | 哈尔滨派特纳生物科技开发有限公司 | Making method for watermelon fruit vinegar |
| CN106983154A (en) * | 2017-04-05 | 2017-07-28 | 天津科技大学 | A kind of preparation method of Natta/chitosan dietary fiber |
| CN109055457A (en) * | 2018-10-08 | 2018-12-21 | 天津科技大学 | A method of bacteria cellulose is produced using grape skin |
| CN109259216A (en) * | 2018-10-12 | 2019-01-25 | 天津科技大学 | A kind of preparation method of edible bacterial cellulose/dietary fiber |
| CN111135336A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Pectin/biological cellulose composite membrane prepared by biological fermentation of whole citrus fruits |
| CN113337411A (en) * | 2021-06-15 | 2021-09-03 | 海南大学 | Method for producing extracellular polysaccharide by fermenting coconut water with Stardatorula and polysaccharide separation and purification method thereof |
| CN114982922A (en) * | 2022-04-06 | 2022-09-02 | 海南博亿生物科技发展有限公司 | Preparation method of coconut gel |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281051A (en) * | 1999-07-19 | 2001-01-24 | 天津轻工业学院 | Process for preparing bacterial cellulose by glucose oxidation and bacillus fermentation |
| CN101234299A (en) * | 2008-03-05 | 2008-08-06 | 许春元 | Bacteria cellulose pervaporation membrane and uses thereof |
-
2010
- 2010-04-01 CN CN 201010137913 patent/CN101805765B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1281051A (en) * | 1999-07-19 | 2001-01-24 | 天津轻工业学院 | Process for preparing bacterial cellulose by glucose oxidation and bacillus fermentation |
| CN101234299A (en) * | 2008-03-05 | 2008-08-06 | 许春元 | Bacteria cellulose pervaporation membrane and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李静: "利用西瓜汁合成细菌纤维素的研究", 《生物技术通报》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102911982B (en) * | 2012-11-23 | 2016-08-03 | 海南椰国食品有限公司 | Fructus Musae purposes in Biological cellulose fermentation culture medium |
| CN102911982A (en) * | 2012-11-23 | 2013-02-06 | 海南椰国食品有限公司 | Application of bananas in biocellulose fermentation media |
| CN102925514A (en) * | 2012-11-23 | 2013-02-13 | 海南椰国食品有限公司 | Application of mangos in bio-cellulose fermentation medium |
| CN102925514B (en) * | 2012-11-23 | 2016-08-03 | 海南椰国食品有限公司 | Fructus Mangifera Indicae purposes in Biological cellulose fermentation culture medium |
| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN104263624A (en) * | 2014-10-23 | 2015-01-07 | 哈尔滨派特纳生物科技开发有限公司 | Making method for watermelon fruit vinegar |
| CN106983154A (en) * | 2017-04-05 | 2017-07-28 | 天津科技大学 | A kind of preparation method of Natta/chitosan dietary fiber |
| CN109055457A (en) * | 2018-10-08 | 2018-12-21 | 天津科技大学 | A method of bacteria cellulose is produced using grape skin |
| CN109259216A (en) * | 2018-10-12 | 2019-01-25 | 天津科技大学 | A kind of preparation method of edible bacterial cellulose/dietary fiber |
| CN111135336A (en) * | 2018-11-06 | 2020-05-12 | 钟宇光 | Pectin/biological cellulose composite membrane prepared by biological fermentation of whole citrus fruits |
| CN111135336B (en) * | 2018-11-06 | 2022-04-08 | 钟宇光 | Pectin/biological cellulose composite membrane prepared by biological fermentation of whole citrus fruits |
| CN113337411A (en) * | 2021-06-15 | 2021-09-03 | 海南大学 | Method for producing extracellular polysaccharide by fermenting coconut water with Stardatorula and polysaccharide separation and purification method thereof |
| CN114982922A (en) * | 2022-04-06 | 2022-09-02 | 海南博亿生物科技发展有限公司 | Preparation method of coconut gel |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101805765B (en) | 2013-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101805765B (en) | Method producing edible colorful bacterial cellulose with abandoned fruit juice or pomace | |
| CN103549385B (en) | Preparation method of agaric ferment | |
| CN101623065B (en) | Preparation method of fermented rice sticks | |
| CN102669780A (en) | Technique for fermentation production of fruit vinegar beverage by utilizing orange peel residue | |
| CN104509907A (en) | Composite plant ferment vinegar beverage and preparation method thereof | |
| CN104256764B (en) | A kind of compound fruit and vegetable juice fermented health drink | |
| CN103734826A (en) | Beetroot juice fermentation type sports beverage and preparation method thereof | |
| CN109303222A (en) | A kind of preparation method of sugar-free no-alcohol type health composite enzyme drink | |
| CN101665373A (en) | Safe and high-efficiency preparation method of master culture medium material of edible fungi | |
| CN106367284B (en) | Liquid submerged fermentation process of health vinegar containing wolfberry, liquorice and apples | |
| CN110663940A (en) | Mulberry and wolfberry enzyme and preparation method thereof | |
| CN108315382A (en) | A method of preparing bacteria cellulose using bean curd yellow pulp water | |
| CN105647756B (en) | A kind of thick wine of selenium-rich cordyceps mulberry health care | |
| CN104757682A (en) | Method for preparing clear ginkgo fruit juice lactic acid beverage by using lactic acid bacteria | |
| CN102266100A (en) | Method for preparing fermented low-alcohol strawberry juice beverage | |
| CN103421665A (en) | Method for producing monascus fruit vinegar | |
| CN107080246A (en) | A kind of plant enzyme and preparation method thereof | |
| CN108624467A (en) | A kind of sugarcane vinegar preparation method improving vinegar fragrance | |
| CN105647755A (en) | Selenium-enriched health care ice wine containing ganoderma lucidum and mulberries | |
| CN105567496A (en) | Golden cordyceps and lotus seed thick liquor | |
| CN105695287B (en) | A kind of selenium-rich agaric mulberry health care black vinegar | |
| CN106222055A (en) | A kind of multi-cultur es co-immobilization fermentation Fructus Caricae vinegar and preparation method thereof | |
| CN110859256A (en) | Levan-rich probiotic health-care sweet potato fermented milk beverage and preparation method thereof | |
| CN106635681A (en) | Preparation method of hericium erinaceus and strawberry compound wine | |
| CN105002231A (en) | Method for preparing bacterial cellulose by biotransforming mulberry leaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SHANGHAI YIFANG RURAL TECHNOLOGY HOLDINGS CO., LTD Free format text: FORMER OWNER: SHANGHAI RONGGANG INDUSTRIAL AND TRADING CO., LTD. |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20110506 Address after: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Applicant after: DONGHUA University Co-applicant after: SHANGHAI YIFANG RURAL TECHNOLOGY HOLDINGS Co.,Ltd. Address before: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Applicant before: Donghua University Co-applicant before: SHANGHAI RONGGANG IND AND TRAD |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Patentee after: DONGHUA University Patentee after: Shanghai Yifang Agricultural Technology Co.,Ltd. Address before: 201620 No. 2999 North Renmin Road, Xincheng District, Shanghai, Songjiang Patentee before: DONGHUA University Patentee before: SHANGHAI YIFANG RURAL TECHNOLOGY HOLDINGS Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder |