CN103789370A - Method for processing bacterial cellulose cloth cutting pieces with fruit juice culture media - Google Patents
Method for processing bacterial cellulose cloth cutting pieces with fruit juice culture media Download PDFInfo
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- CN103789370A CN103789370A CN201410031330.7A CN201410031330A CN103789370A CN 103789370 A CN103789370 A CN 103789370A CN 201410031330 A CN201410031330 A CN 201410031330A CN 103789370 A CN103789370 A CN 103789370A
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Abstract
The invention discloses a method for processing bacterial cellulose cloth cutting pieces with fruit juice culture media. The method comprises the following steps: putting the cloth structured cutting pieces into a closed bacterial cellulose culture pond filled with the fruit juice culture media, injecting air, oxygenated air and oxygen into the cloth structured cutting pieces via a pipeline to satisfy the requirement on growth of bacterial celluloses on the surfaces of the cloth structured cutting pieces, stripping two bacterial cellulose cloth cutting piece wet films with the same model from the exterior of the cloth structured cutting pieces after fermentation is completed, removing the culture media and impurities on the surfaces of the films, treating the bacterial cellulose cloth cutting piece wet films with a sodium hydroxide solution to remove thalli and the residual culture media in the liquid films, washing the bacterial cellulose cloth cutting piece wet films with distilled water until the pH value is 7 and sewing the dried bacterial cellulose cloth cutting piece wet films to prepare clothes or bedding articles. The defects that the bacterial cellulose cloth production flow is long and industrialization and processing can not be achieved are overcome by adopting the direct bacterial cellulose cloth preparation technology combining three-layer composite sheet extra-large oxygen supply area design and timing and concentration-based staged oxygen supply and continuous oxygen supply design.
Description
Technical field
The invention belongs to the preparing technical field of clothing cutting plate, be specifically related to a kind of method of utilizing the bacterium direct production cellulose materials of fruit juice substratum to prepare cloth cut-parts.
Background technology
Bacteria cellulose is extensively present in nature, just there is special production period in the Chinese Qin Dynasty, but it is mainly used in drink, also a large amount of popular in Europe at present, Japan, the countries and regions such as the Soviet Union, traditional wine vinegar also has the generation of bacteria cellulose in producing, but really bacteria cellulose is separated and is applied to suitability for industrialized production processing, only has so far the history of more than 100 year, abundant not due to its physical property is understood, thus application is restricted.The recently more than ten years, along with to the understanding in depth and the improvement of fermentation condition of its biosynthesizing mechanism, accelerate the industrial application of bacteria cellulose.
Bacteria cellulose (Bacterial Cellulose, BC) is to be found in the time cultivating acetobacter by Adrian Brown for 1886.In substratum, contain the compositions such as carbon source, nitrogenous source, organic acid, inorganic salt, wherein Carbon and nitrogen sources is two kinds of topmost compositions.The natural medium of BC is mainly renewable resources and waste liquid, comprise the waste water of food-processing, beet and sugarcane and the liquid food waste material of not processing, acid-hydrolyzed Rhizoma amorphophalli powder, the sweet potato slurry of saccharification processing, the hemicellulose and the fruit juice etc. that in atmosphere acetic acid pulping waste liquor, are hydrolyzed.Conventional comprises that fruit juice, saccharine material, starch and Mierocrystalline cellulose etc. are several, maximum with fruit juice.Except Sucus Cocois, other fruit and fruit syrup also can be used as fermentation raw material and produce BC, utilize various fruit, comprise that orange, pineapple, apple, Japanese pear and grape etc. are as fermention medium, add after nitrogenous source, the output of BC obviously improves, and experiment finds that orange and two kinds of fruit juice of Japanese pear are to produce the optimal raw material of BC in addition.To concentrate Fructus actinidiae chinensis juice, orange juice, Pulp Citrulli juice and watermelon husk juice, pineapple juice and pineapple skin juice as raw material, carry out acetic bacteria fermentation, can cultivate the BC of high-quality.
Bacteria cellulose is the natural polymers being formed by connecting by β-Isosorbide-5-Nitrae-glycosidic link, and the chemical purity of bacteria cellulose is very high, has good biodegradability; It has exquisite natural ultra micro fibrous reticular structure, this reticulated structure is to be made up of a kind of natural nanofiber forming, its diameter is only 1. 5 nm, the thinnest in current natural fiber, and bacteria cellulose is to exist with 100% cellulosic form, very high purity, and there is good permeability, high-tensile and splendid proterties and maintain the characteristics such as ability.At present the research of bacteria cellulose is mainly concentrated on the aspects such as tissue engineering bracket, bone support, cartilage frame, artificial blood vessel, artificial skin, pharmaceutical carrier, gauze, bandage and " bandage " traumatology dressing commodity that added value is higher, also there is in other respects some commercial potential widely.
Bacteria cellulose film and other artificial skins with traumatology dressing compare, maximum feature be under moisture conditions physical strength high, liquid, gas and electrolyte are had to good permeability, good with skin-friendliness, nonirritant, is conducive to skin histology growth, is also extraordinary slow releasing carrier of medication.Bacteria cellulose can absorb rapidly wound blood and tissue juice as adhesive bandage material, prevents wound infection suppuration, can promote wound healing and ease the pain near tissue regeneration chronic wounds provide moistening environment again.Simultaneously Mierocrystalline cellulose not can with wound adhesion, can not cause secondary injury, while peeling off, do not have residual yet.
Bacteria cellulose has bioaffinity, biocompatibility, biological fitness and good biodegradability.Excellent performance has been that occurring in nature is the abundantest and have the natural macromolecular material of biodegradability by internationally recognized Mierocrystalline cellulose, and bacteria cellulose becomes the focus of present material research.Be limited by cost high, yield poorly, obstruction that technical transform disconnects, bacteria cellulose slowly fails to realize scale industrialization in textile garment material product.
Summary of the invention
Technical problem to be solved by this invention is that the Production Flow Chart of directed toward bacteria Mierocrystalline cellulose cloth is long, cannot process and industrialized defect, and a kind of working method of utilizing the cut-parts of fruit juice substratum bacterium direct production cellulose materials cloth is provided.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
The working method of fruit juice substratum bacteria cellulose cloth of the present invention cut-parts, comprises the following steps: the preparation of (1) seed culture medium: take water as benchmark, add the component of following massfraction: glucose 6 ~ 10%, yeast extract paste 0.1 ~ 1%, K
2hPO
43H
2o 0.1 ~ 0.5% and MgSO
47H
2o 1 ~ 3%, take water as benchmark, adding volume fraction is 1 ~ 3% dehydrated alcohol, under the condition of 115 ~ 125 ℃, sterilizing 25 ~ 40min; The preparation of fruit juice fermentation substratum: take fruit juice as benchmark, add the yeast extract paste of 7 ~ 9g in every liter of fruit juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring or two ring activation, under the condition of 25 ~ 35 ℃ and 50 ~ 70r/min, fully vibration, makes bacterium liquid even, cultivation 20 ~ 30h; In the airtight pond of acetobacter xylinum access fermention medium, the inoculum size of described acetobacter xylinum is 7 ~ 9% of fermention medium volume;
(3) fabric construction cut-parts are dipped vertically in the airtight pond that acetobacter xylinum fermention medium is housed, under the condition of 30 ~ 37 ℃ of airtight pond temperature, at interval of 10 ~ 30min, air, oxygenation air and oxygen continuous circulation are injected to fabric construction cut-parts by pipeline, fabric construction cut-parts surface is carried out air, aerobic air and total oxygen continous way bacteria cellulose and is cultivated in airtight pond, under the condition of 30 ~ 37 ℃, cultivate 7 ~ 9d, acetobacter xylinum is at fabric construction cut-parts outside surface biosynthesizing bacteria cellulose cloth cut-parts wet film;
(4) from fabric construction cut-parts, can separate the bacteria cellulose cloth cut-parts wet film of two cover same models after having fermented, and water rinses until except striping surface medium and impurity;
(5) the bacteria cellulose cloth cut-parts wet film after step (4) being rinsed well boils to 80 ℃ with 0.1mol/L sodium hydroxide solution, continue to boil 20min, remove thalline and residual substratum, then flowing water rinses, and after drying, being dried, obtains the cut-parts of bacteria cellulose cloth.
In described step (1), fruit juice fermentation substratum is any one in Sucus Cocois fermention medium, apple juice fermentation substratum, pear juice fermention medium, pineapple juice fermention medium or fermentation of watermelon juice substratum.
Fabric construction cut-parts in described step (3) are apparel construction cut-parts or the cut-parts of bedclothes structure.
The three-layer composite structure sheet material forming is suppressed in fabric construction cut-parts in described step (3) through grain roller by surface layer, sandwich layer and bottom.
Described surface layer is 0.01 ~ 10 μ m by fineness, and grammes per square metre is 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor suppress one or more compound the making in film, described sandwich layer is 2 ~ 2000 μ m fineness by fineness, grammes per square metre is 20 ~ 2000g/ m
2non-woven fabrics make, described bottom is 0.01 ~ 10 μ m by fineness, grammes per square metre is at 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor suppress one or more compound the making in film.
Described three-layer composite structure sheet material, according to lines, is spent type, and fabric construction cut-parts compacting forms, the lines of the three-layer composite structure sheet material that compacting forms, and flower type, leaves communication passage between fabric construction cut-parts.
Oxygenation air in described step (3) is in every litres of air, to pass into 0.05 ~ 0.1 liter of oxygen.
In described step (5), oven dry and dry actual conditions are 50 ~ 80 ℃ of temperature, humidity 50%, and the time is 5 ~ 10h.
Grain roller in the time being pressed into three-layer composite structure sheet material, according to certain lines, flower type, apparel construction cut-parts or the cut-parts of bedclothes structure are suppressed, the three-layer composite structure sheet material with hydrophobic permeable function has decorative pattern, in decorative pattern, be interconnected between decorative pattern; The three-layer composite structure sheet material of hydrophobic permeable function has lines, in lines, be interconnected between lines; The three-layer composite structure sheet material with hydrophobic permeable function has apparel construction or bedclothes structure, in apparel construction, be interconnected between apparel construction, apparel construction cut-parts can be upper coat-sleeve sheets, the cut-parts such as anter, rear panel, collar, pocket, also can be the cut-parts such as trousers sheet, waist sheet, trouser pocket sheet, can also be the cut-parts such as cap, scarf, gloves; In bedclothes structure, be interconnected between bedclothes structure, the cut-parts of bedclothes structure can be the structure cut-parts such as sheet, quilt cover, Pillow cases.
Beneficial effect of the present invention: the present invention utilizes three layers of composite sheet super large oxygen supply area design, timing concentration sectional oxygen supply, the method that supplies continuously aerobe fermentation automatic control equipment and standing for fermentation technique to combine improves bacteria cellulose output, reduce costs, simultaneously in conjunction with fruit juice substratum bacteria cellulose clothing cutting plate or the direct technology of preparing of bedclothes cut-parts, bacteria cellulose clothes are solved or the Production Flow Chart of bacteria cellulose bedclothes is long, cannot process and industrialized defect, the bacteria cellulose clothing cutting plate or the bedclothes cut-parts that obtain have good permeability, high-tensile and splendid proterties maintain ability.
Embodiment
Embodiment 1
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: take water as benchmark, add the component of following massfraction: glucose 7%, yeast extract paste 0.5%, K
2hPO
43H
2o 0.15%, MgSO
47H
2o1.5% and dehydrated alcohol, the volume of dehydrated alcohol is water 2%, under the condition of 121 ℃, sterilizing 30min; The preparation of fruit juice fermentation substratum: take Sucus Cocois as benchmark, add 7g yeast extract paste in every liter of Sucus Cocois;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under the condition of 30 ℃ and 60r/min, fully vibration, makes bacterium liquid even, cultivates 24h; In the airtight pond of 7mL acetobacter xylinum access 100mL fermention medium;
(3) be 0.01 μ m by fineness, grammes per square metre is 1g/ m
2pP non-woven fabrics do surface layer, fineness is 2 μ m fineness, grammes per square metre is 20g/m
2non-woven fabrics do sandwich layer, be 0.01 μ m by fineness, grammes per square metre is at 1g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool twill lines, two anti-S blessing flower types, upper garment sleeve, anter, rear panel, the apparel construction such as pocket and collar, lines, flower type, apparel construction, between cut-parts, leave communication passage, above structure flower matrix material forms apparel construction cut-parts, apparel construction cut-parts are dipped vertically in the airtight pond 3 that acetobacter xylinum fermention medium is housed, be under the condition of 30 ℃ in airtight pond temperature, by intake ducting at interval of 10min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, to pass into 0.05 liter of oxygen, air is carried out on apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, leave standstill and cultivate 7d at 30 ℃, acetobacter xylinum is at apparel construction cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) from apparel construction cut-parts, can separate the bacteria cellulose clothing cutting plate wet film of two cover same models after having fermented, and water rinses until except striping surface medium and impurity;
(5) the bacteria cellulose clothing cutting plate wet film after step (4) being rinsed well boils to 80 ℃ with 0.1mol/L sodium hydroxide solution, continue to boil 20min, remove thalline and residual substratum, then flowing water rinses, at 50 ℃, under the condition of humidity 50%, dry 5h, after being dried, obtain bacteria cellulose clothing cutting plate.
Embodiment 2
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: take water as benchmark, add the component of following massfraction: glucose 6%, yeast extract paste 0.1%, K
2hPO
43H
2o 0.1% and MgSO
47H
2o 1%, then add dehydrated alcohol, the volume of dehydrated alcohol is water 1%, under the condition of 115 ℃, sterilizing 25min; The preparation of fruit juice fermentation substratum: take Sucus Mali pumilae as benchmark, add 8g yeast extract paste in every liter of Sucus Mali pumilae;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under the condition of 25 ℃ and 70r/min, fully vibration, makes bacterium liquid even, cultivates 20h, in the airtight pond of 8mL acetobacter xylinum access 100mL fermention medium;
(3) be 10 μ m by fineness, grammes per square metre is 30g/ m
2pE non-woven fabrics do surface layer, fineness is 2000 μ m, grammes per square metre is 2000g/m
2non-woven fabrics do sandwich layer, be 10 μ m by fineness, grammes per square metre is at 30g/ m
2pE non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool plain weave lines, pine needle flower type, trousers sheet, want sheet, and the apparel construction such as trouser pocket sheet, lines, flower type, apparel construction, between cut-parts, leave communication passage, above structure flower matrix material forms apparel construction cut-parts, apparel construction cut-parts are dipped vertically in the airtight pond that acetobacter xylinum fermention medium is housed, be under the condition of 37 ℃ in airtight pond temperature, by intake ducting at interval of 30min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, to pass into 0.1 liter of oxygen, air is carried out on apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, leave standstill and cultivate 8d at 37 ℃, acetobacter xylinum is at apparel construction cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) from apparel construction cut-parts, can separate the bacteria cellulose clothing cutting plate wet film of two cover same models after having fermented, and water rinses until except striping surface medium and impurity;
(5) the bacteria cellulose clothing cutting plate wet film after step (4) being rinsed well boils to 80 ℃ with 0.1mol/L sodium hydroxide solution, continue to boil 20min, remove thalline and residual substratum, then flowing water rinses, at 80 ℃, under the condition of humidity 50%, dry 10h, after being dried, obtain bacteria cellulose clothing cutting plate.
Embodiment 3
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: take water as benchmark, add the component of following massfraction: glucose 10%, yeast extract paste 1%, K
2hPO
43H
2o 0.5% and MgSO
47H
2o 3%, then add dehydrated alcohol, dehydrated alcohol volume is water 3%, under the condition of 125 ℃, sterilizing 40min; The preparation of fruit juice fermentation substratum: take pineapple juice as benchmark, add 9g yeast extract paste in every liter of pineapple juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under the condition of 35 ℃ and 50r/min, fully vibration, makes bacterium liquid even, cultivates 30h, in the airtight pond of 9mL acetobacter xylinum access 100mL fermention medium;
(3) be 6 μ m by fineness, grammes per square metre is 25g/ m
2pS non-woven fabrics do surface layer, fineness is 200 μ m, grammes per square metre is 200g/m
2non-woven fabrics do sandwich layer, be 6 μ m by fineness, grammes per square metre is at 25g/ m
2pS non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool twill lines, two anti-S blessing flower types, the structure cut-parts such as sheet size, lines, flower type, sheet structure, between cut-parts, leave communication passage, above structure flower matrix material forms the cut-parts of sheet structure, the cut-parts of sheet structure are dipped vertically in the airtight pond that acetobacter xylinum fermention medium is housed, be under the condition of 35 ℃ in airtight pond temperature, by intake ducting at interval of 20min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, to pass into 0.08 liter of oxygen, air is carried out on apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, leave standstill and cultivate 9d at 35 ℃, acetobacter xylinum is at sheet structure cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) from the cut-parts of sheet structure, can separate the bacteria cellulose clothing cutting plate wet film of two cover same models after having fermented, and water rinses until except striping surface medium and impurity;
(5) the bacteria cellulose sheet cut-parts wet film after step (4) being rinsed well boils to 80 ℃ with 0.1mol/L sodium hydroxide solution, continue to boil 20min, remove thalline and residual substratum, then flowing water rinses, at 65 ℃, under the condition of humidity 50%, dry 8h, after being dried, obtain the cut-parts of bacteria cellulose sheet.
Embodiment 4
The working method of fruit juice substratum bacteria cellulose clothing cutting plate: be a 1 μ m by fineness, grammes per square metre is at 10g/ m
2pP non-woven fabrics do surface layer, fineness is 20 μ m fineness, grammes per square metre is that the non-woven fabrics of every square of 200g/ does sandwich layer, by fineness, at 1 μ m, grammes per square metre is at 10g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool twill lines, two anti-S blessing flower types, the apparel constructions such as upper garment sleeve, anter, rear panel, pocket and collar, lines, flower type, leaves communication passage between apparel construction, cut-parts, and above structure flower matrix material forms apparel construction cut-parts.By above apparel construction cut-parts according to certain arrangement regulation vertical out-fall in an airtight pond 3 of culturing bacterium Mierocrystalline cellulose, in apparel construction cut-parts, inject air, oxygenation air through intake ducting, oxygen, meet the cellulosic growth of apparel construction cut-parts surface bacteria, in airtight pond, carry out apparel construction cut-parts surface bacteria Mierocrystalline cellulose and cultivate adding containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 7%(v/v) inoculum size.At apparel construction cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 30 ℃ of constant temperature, through fermentation in 7 days, after having fermented, apparel construction cut-parts surface growth goes out bacteria cellulose film, from apparel construction cut-parts, can separate the bacteria cellulose clothing cutting plate of two cover same models, the bacteria cellulose clothing cutting plate wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose clothing cutting plate wet film is dried with drying plant respectively, make and sew on clothing through sewing tech.
Embodiment 5
The working method of fruit juice substratum bacteria cellulose clothing cutting plate: be 5 μ m by fineness, grammes per square metre is at 15g/ m
2pP non-woven fabrics do surface layer, fineness is 20 μ m fineness, grammes per square metre is 1000g/ m
2non-woven fabrics do sandwich layer, by fineness, at 5 μ m, grammes per square metre is at 15g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool plain weave lines, pine needle flower type, trousers sheet, wants the apparel construction such as sheet and trouser pocket sheet, lines, flower type, leaves communication passage between apparel construction, cut-parts, and above structure flower matrix material forms apparel construction cut-parts.By above apparel construction cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in apparel construction cut-parts, inject air, oxygenation air through admission passage, oxygen, meet the cellulosic growth of apparel construction cut-parts surface bacteria, in airtight pond, carry out apparel construction cut-parts surface bacteria Mierocrystalline cellulose and cultivate adding containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 8%(v/v) inoculum size.At apparel construction cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 30 ℃ of constant temperature, through fermentation in 8 days, after having fermented, apparel construction cut-parts surface growth goes out bacteria cellulose film, from apparel construction cut-parts, can separate the bacteria cellulose clothing cutting plate of two cover same models, the bacteria cellulose clothing cutting plate wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose clothing cutting plate wet film is dried with drying plant respectively, make seam trousers through sewing tech.
Embodiment 6
The working method of fruit juice substratum bacteria cellulose clothing cutting plate: be 10 μ m by fineness, grammes per square metre is at 30g/ m
2pP non-woven fabrics do surface layer, fineness is 1000 μ m fineness, grammes per square metre is 2000g/ m
2non-woven fabrics do sandwich layer, by fineness, at 10 μ m, grammes per square metre is at 30g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool satin weave lines, feather flower type, glove construction cut-parts, lines, flower type, leaves communication passage between glove construction, cut-parts, and above structure flower matrix material forms glove construction cut-parts.By above glove construction cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in glove construction cut-parts, inject air, oxygenation air through admission passage, oxygen, meet the growth that takes glove construction cut-parts bacteria cellulose, in airtight pond, carry out glove construction cut-parts bacteria cellulose and cultivate adding containing the substratum of acetobacter xylinum, wherein bacterial strain M438 fermention medium is 9%(v/v) inoculum size.At glove construction cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 30 ℃ of constant temperature, through fermentation in 9 days, after having fermented, glove construction cut-parts surface growth goes out bacteria cellulose film, from glove construction cut-parts, can separate the bacteria cellulose clothing cutting plate of two cover same models, the bacteria cellulose gloves cut-parts wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose gloves cut-parts wet film is dried with drying plant respectively, make seam gloves through sewing tech.
Embodiment 7
The working method of fruit juice substratum bacteria cellulose bedclothes cut-parts: be a 1 μ m by fineness, grammes per square metre is at 10 g/ m
2pP non-woven fabrics do surface layer, fineness is 20 μ m fineness, grammes per square metre is 200 g/ m
2non-woven fabrics do sandwich layer, by fineness, at 1 μ m, grammes per square metre is at 10 g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool twill lines, two anti-S blessing flower types, the structure cut-parts such as sheet size, lines, flower type, sheet structure, between cut-parts, leave communication passage, above structure flower matrix material forms the cut-parts of sheet structure, by the cut-parts of above sheet structure according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in the cut-parts of sheet structure, inject air through admission passage, oxygenation air, oxygen, meet the cellulosic growth of sheet structure cut-parts surface bacteria, in airtight pond, carry out sheet structure cut-parts surface bacteria Mierocrystalline cellulose and cultivate adding containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 7%(v/v) inoculum size.At sheet structure cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 30 ℃ of constant temperature, through fermentation in 7 days, after having fermented, sheet structure cut-parts surface growth goes out bacteria cellulose film, from the cut-parts of sheet structure, can separate the bacteria cellulose sheet cut-parts of two cover same models, the bacteria cellulose sheet cut-parts wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose sheet cut-parts wet film is dried with drying plant respectively, make sheet through sewing tech.
Embodiment 8
The working method of fruit juice substratum bacteria cellulose bedclothes cut-parts: be 5 μ m by fineness, grammes per square metre is at 15 g/ m
2pP non-woven fabrics do surface layer, fineness is 1000 μ m fineness, grammes per square metre is 100 g/ m
2non-woven fabrics do sandwich layer, by fineness, at 5 μ m, grammes per square metre is at 15 g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool plain weave lines, pine needle flower type, the structures such as pillowcase, lines, flower type, leaves communication passage between pillowcase structure, cut-parts, and above structure flower matrix material forms the cut-parts of pillowcase structure.By the cut-parts of above pillowcase structure according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in the cut-parts of pillowcase structure, inject air, oxygenation air through admission passage, oxygen, meet the cellulosic growth of pillowcase structure cut-parts surface bacteria, in airtight pond, carry out pillowcase structure cut-parts surface bacteria Mierocrystalline cellulose and cultivate adding containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 8%(v/v) inoculum size.At pillowcase structure cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 35 ℃ of constant temperature, through fermentation in 8 days, after having fermented, pillowcase structure cut-parts surface growth goes out bacteria cellulose film, from the cut-parts of pillowcase structure, can separate the bacteria cellulose pillowcase cut-parts of two (i.e. a pillowcase) same models, the bacteria cellulose pillowcase cut-parts wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose pillowcase cut-parts wet film is dried with drying plant respectively, make pillowcase through sewing tech.
Embodiment 9
The working method of fruit juice substratum bacteria cellulose bedclothes cut-parts: be 10 μ m by fineness, grammes per square metre is at 30 g/ m
2pP non-woven fabrics do surface layer, fineness is 2000 μ m fineness, grammes per square metre is 2000 g/ m
2non-woven fabrics do sandwich layer, by fineness, at 10 μ m, grammes per square metre is at 30 g/ m
2pP non-woven fabrics do bottom, be pressed into three layers of colored matrix material through grain roller, according to UNICOM's structure design flower matrix material, flower matrix material tool satin weave lines, feather flower type, by cover structure cut-parts, lines, flower type, is left communication passage between cover structure, cut-parts, and above structure flower matrix material forms by cover structure cut-parts.Will be above by cover structure cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in by cover structure cut-parts, inject air, oxygenation air through admission passage, oxygen, meet by the growth of cover structure cut-parts bacteria cellulose, to add in airtight pond and carry out being cultivated by cover structure cut-parts bacteria cellulose containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium be 9%(v/v) inoculum size.By cover structure cut-parts outside surface biosynthesizing bacteria cellulose film, leave standstill and cultivate at 37 ℃ of constant temperature, through fermentation in 9 days, after having fermented, gone out bacteria cellulose film by cover structure cut-parts surface growth, from being separated the bacteria cellulose quilt cover cut-parts of two (i.e. a quilt cover) same models cover structure cut-parts, the bacteria cellulose gloves cut-parts wet film water separating repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 ℃, time is 20min, remove thalline and residual substratum in liquid film, rinse with flowing water, through distilled water flushing to pH be 7, bacteria cellulose quilt cover cut-parts wet film is dried with drying plant respectively, make quilt cover through sewing tech.
Claims (8)
1. a working method for fruit juice substratum bacteria cellulose cloth cut-parts, is characterized in that comprising the following steps: the preparation of (1) seed culture medium: take water as benchmark, add the component of following massfraction: glucose 6 ~ 10%, yeast extract paste 0.1 ~ 1%, K
2hPO
43H
2o 0.1 ~ 0.5% and MgSO
47H
2o 1 ~ 3%, take water as benchmark, adding volume fraction is 1 ~ 3% dehydrated alcohol, under the condition of 115 ~ 125 ℃, sterilizing 25 ~ 40min; The preparation of fruit juice fermentation substratum: take fruit juice as benchmark, add the yeast extract paste of 7 ~ 9g in every liter of fruit juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring or two ring activation, under the condition of 25 ~ 35 ℃ and 50 ~ 70r/min, fully vibration, makes bacterium liquid even, cultivation 20 ~ 30h; In the airtight pond of acetobacter xylinum access fermention medium, the inoculum size of described acetobacter xylinum is 7 ~ 9% of fermention medium volume;
(3) fabric construction cut-parts are dipped vertically in the airtight pond that acetobacter xylinum fermention medium is housed, under the condition of 30 ~ 37 ℃ of airtight pond temperature, at interval of 10 ~ 30min, air, oxygenation air and oxygen continuous circulation are injected to fabric construction cut-parts by pipeline, fabric construction cut-parts surface is carried out air, aerobic air and total oxygen continous way bacteria cellulose and is cultivated in airtight pond, under the condition of 30 ~ 37 ℃, cultivate 7 ~ 9d, acetobacter xylinum is at fabric construction cut-parts outside surface biosynthesizing bacteria cellulose cloth cut-parts wet film;
(4) from fabric construction cut-parts, can separate the bacteria cellulose cloth cut-parts wet film of two cover same models after having fermented, and water rinses until except striping surface medium and impurity;
(5) the bacteria cellulose cloth cut-parts wet film after step (4) being rinsed well boils to 80 ℃ with 0.1mol/L sodium hydroxide solution, continue to boil 20min, remove thalline and residual substratum, then flowing water rinses, and after drying, being dried, obtains the cut-parts of bacteria cellulose cloth.
2. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: in described step (1), fruit juice fermentation substratum is any one in Sucus Cocois fermention medium, apple juice fermentation substratum, pear juice fermention medium, pineapple juice fermention medium or fermentation of watermelon juice substratum.
3. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: the fabric construction cut-parts in described step (3) are apparel construction cut-parts or the cut-parts of bedclothes structure.
4. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: the three-layer composite structure sheet material forming is suppressed in the fabric construction cut-parts in described step (3) through grain roller by surface layer, sandwich layer and bottom.
5. the working method of fruit juice substratum bacteria cellulose cloth according to claim 3 cut-parts, is characterized in that: described surface layer is 0.01 ~ 10 μ m by fineness, and grammes per square metre is 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor suppress one or more compound the making in film, described sandwich layer is 2 ~ 2000 μ m fineness by fineness, grammes per square metre is 20 ~ 2000g/ m
2non-woven fabrics make, described bottom is 0.01 ~ 10 μ m by fineness, grammes per square metre is at 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor suppress one or more compound the making in film.
6. the working method of fruit juice substratum bacteria cellulose cloth according to claim 3 cut-parts, it is characterized in that: described three-layer composite structure sheet material is according to lines, flower type, fabric construction cut-parts compacting forms, the lines of the three-layer composite structure sheet material that compacting forms, flower type, leaves communication passage between fabric construction cut-parts.
7. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: the oxygenation air in described step (3) is in every litres of air, to pass into 0.05 ~ 0.1 liter of oxygen.
8. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: in described step (5), oven dry and dry actual conditions are 50 ~ 80 ℃ of temperature, humidity 50%, and the time is 5 ~ 10h.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN106099182A (en) * | 2016-08-05 | 2016-11-09 | 宁波高智科技咨询服务有限公司 | A kind of lithium battery bacterial cellulose gel method for preparing polymer electrolytes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031956A (en) * | 2014-06-05 | 2014-09-10 | 陕西科技大学 | Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium |
| CN106099182A (en) * | 2016-08-05 | 2016-11-09 | 宁波高智科技咨询服务有限公司 | A kind of lithium battery bacterial cellulose gel method for preparing polymer electrolytes |
| CN106099182B (en) * | 2016-08-05 | 2018-08-03 | 宁波高智科技咨询服务有限公司 | A kind of lithium battery bacterial cellulose gel method for preparing polymer electrolytes |
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