CN103789370B - The working method of a kind of fruit juice substratum bacteria cellulose cloth cut-parts - Google Patents
The working method of a kind of fruit juice substratum bacteria cellulose cloth cut-parts Download PDFInfo
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- CN103789370B CN103789370B CN201410031330.7A CN201410031330A CN103789370B CN 103789370 B CN103789370 B CN 103789370B CN 201410031330 A CN201410031330 A CN 201410031330A CN 103789370 B CN103789370 B CN 103789370B
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Abstract
The invention discloses the working method of a kind of fruit juice substratum bacteria cellulose cloth cut-parts, fabric construction cut-parts being placed on one airtight is equipped with in the bacteria cellulose cultivation pool of fruit juice substratum, air is injected by the road in fabric construction cut-parts, oxygenation air, oxygen, meet the cellulosic growth of fabric construction cut-parts surface bacteria, the bacteria cellulose cloth cut-parts wet film of two cover same models can be separated from fabric construction cut-parts after having fermented, except striping surface medium and impurity, with the thalline in sodium hydroxide solution process removal liquid film and remaining medium, be 7 through distilled water flushing to pH, bacteria cellulose cloth cut-parts wet film makes seam clothes or bedclothes through sewing tech after drying.Three layers of composite sheet super large oxygen supply area design of the present invention, timing, the design of concentration sectional oxygen supply, continuously oxygen supply, in conjunction with the direct technology of preparing of bacteria cellulose cloth, solve the Production Flow Chart of bacteria cellulose cloth long, cannot the defect of industrialization and processing.
Description
Technical field
The invention belongs to the preparing technical field of clothing cutting plate, be specifically related to a kind of method utilizing the bacterium direct production cellulose materials of fruit juice substratum to prepare cloth cut-parts.
Background technology
Bacteria cellulose is extensively present in nature, in the Chinese Qin Dynasty, just there is special production in period, but it is mainly used in drink, also popular in Europe in a large number at present, Japan, the countries and regions such as the Soviet Union, traditional wine vinegar also has the generation of bacteria cellulose in producing, but is really separated by bacteria cellulose and is applied to suitability for industrialized production processing, only has the history of more than 100 year so far, abundant not owing to understanding its physical property, thus application is restricted.The nearest more than ten years, along with understanding in depth and the improvement of fermentation condition its biosynthesizing mechanism, accelerate the industrial application of bacteria cellulose.
Bacteria cellulose (BacterialCellulose, BC) is found when cultivating acetobacter by AdrianBrown for 1886.Containing compositions such as carbon source, nitrogenous source, organic acid, inorganic salt in substratum, wherein Carbon and nitrogen sources is two kinds of topmost compositions.The natural medium of BC is mainly renewable resources and waste liquid, comprise the waste water of food-processing, the liquid food waste material of beet and sugarcane and no process, acid-hydrolyzed Rhizoma amorphophalli powder, the sweet potato slurry of saccharification process, the hemicellulose be hydrolyzed in air acetic acid pulping waste liquor and fruit juice etc.Conventional to comprise fruit juice, saccharine material, starch and Mierocrystalline cellulose etc. several, maximum with fruit juice.Except Sucus Cocois, other fruit and fruit syrup also can be used as fermentation raw material to produce BC, utilize various fruit, comprise orange, pineapple, apple, Japanese pear and grape etc. as fermention medium, after adding nitrogenous source, the output of BC significantly improves, and experiment finds that the optimal raw material of BC produced by orange and Japanese pear two kinds of fruit juice in addition.With concentrated Fructus actinidiae chinensis juice, orange juice, Pulp Citrulli juice and watermelon husk juice, pineapple juice and pineapple skin juice for raw material, carry out acetic bacteria fermentation, the BC of high-quality can be cultivated.
Bacteria cellulose is the natural polymers be formed by connecting by β-Isosorbide-5-Nitrae-glycosidic link, and the chemical purity of bacteria cellulose is very high, has good biodegradability; It has exquisite natural ultra-microfibers reticulated structure, this reticulated structure is made up of a kind of natural nanofiber formed, its diameter is only 1.5nm, the thinnest in current natural fiber, and bacteria cellulose exists with 100% cellulosic form, very high purity, and there is the characteristics such as good permeability, high-tensile and splendid proterties maintenance ability.At present the aspects such as the higher tissue engineering bracket of added value, bone support, cartilage frame, artificial blood vessel, artificial skin, pharmaceutical carrier, gauze, bandage and " bandage " traumatology dressing commodity are mainly concentrated on to the research of bacteria cellulose, in other respects also there is some commercial potential widely.
Bacteria cellulose film is compared with traumatology dressing with other artificial skins, maximum feature be under moisture conditions physical strength high, to have liquid, gas and electrolyte good permeability and skin-friendliness good, nonirritant, being conducive to skin histology growth, is also extraordinary slow releasing carrier of medication.Bacteria cellulose can absorb rapidly wound blood and tissue juice as adhesive bandage material, prevents wound infection from suppurating, and moistening environment can be provided again for the tissue regeneration near chronic wounds to promote wound healing and ease the pain.Simultaneously Mierocrystalline cellulose can not with wound adhesion, can not secondary injury be caused, also not have residual during stripping.
Bacteria cellulose has bioaffinity, biocompatibility, biological fitness and good biodegradability.Excellent performance has been that occurring in nature is the abundantest and have the natural macromolecular material of biodegradability by internationally recognized Mierocrystalline cellulose, and bacteria cellulose becomes the focus of present material research.Be limited by cost high, yield poorly, technical transform disconnect obstruction, bacteria cellulose slowly fails to realize scale industrialization in textile garment material product.
Summary of the invention
Technical problem to be solved by this invention is that the Production Flow Chart of directed toward bacteria Mierocrystalline cellulose cloth is long, cannot process and industrialized defect, and provide a kind of working method utilizing the cut-parts of fruit juice substratum bacterium direct production cellulose materials cloth.
For solving the problems of the technologies described above, the present invention by the following technical solutions:
The working method of fruit juice substratum bacteria cellulose cloth of the present invention cut-parts, comprises the following steps: the preparation of (1) seed culture medium: be benchmark with water, adds the component of following masses mark: glucose 6 ~ 10%, yeast extract paste 0.1 ~ 1%, K
2hPO
43H
2o0.1 ~ 0.5% and MgSO
47H
2o1 ~ 3% is benchmark with water, adds the dehydrated alcohol that volume fraction is 1 ~ 3%, under the condition of 115 ~ 125 DEG C, and sterilizing 25 ~ 40min; The preparation of fruit juice fermentation substratum: be benchmark with fruit juice, adds the yeast extract paste of 7 ~ 9g in often liter of fruit juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring or the activation of two rings, under the condition of 25 ~ 35 DEG C and 50 ~ 70r/min, fully vibrates, makes bacterium liquid even, cultivation 20 ~ 30h; In the airtight pond of acetobacter xylinum access fermention medium, the inoculum size of described acetobacter xylinum is 7 ~ 9% of fermention medium volume;
(3) fabric construction cut-parts are dipped vertically into are equipped with in the airtight pond of acetobacter xylinum fermention medium, under the condition of airtight pond temperature 30 ~ 37 DEG C, at interval of 10 ~ 30min, air, oxygenation air and oxygen continuous circulation are injected fabric construction cut-parts by pipeline, fabric construction cut-parts surface is carried out air, aerobic air and total oxygen continous way bacteria cellulose and is cultivated in airtight pond, under the condition of 30 ~ 37 DEG C, cultivate 7 ~ 9d, acetobacter xylinum is at fabric construction cut-parts outside surface biosynthesizing bacteria cellulose cloth cut-parts wet film;
(4) the bacteria cellulose cloth cut-parts wet film of two cover same models can be separated after having fermented from fabric construction cut-parts, and rinse with water until except striping surface medium and impurity;
(5) step (4) being rinsed the bacteria cellulose cloth cut-parts wet film 0.1mol/L sodium hydroxide solution well boils to 80 DEG C, continue to boil 20min, removing thalline and remaining medium, then running water, obtain the cut-parts of bacteria cellulose cloth after oven dry, drying.
In described step (1), fruit juice fermentation substratum is any one in Sucus Cocois fermention medium, apple juice fermentation substratum, pear juice fermention medium, pineapple juice fermention medium or fermentation of watermelon juice substratum.
Fabric construction cut-parts in described step (3) are apparel construction cut-parts or the cut-parts of bedclothes structure.
The three-layer composite structure sheet material that fabric construction cut-parts in described step (3) are suppressed through grain roller by surface layer, sandwich layer and bottom.
Described surface layer is 0.01 ~ 10 μm by fineness, and grammes per square metre is 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor's one or more compounds of suppressing in film make, described sandwich layer is 2 ~ 2000 μm of fineness by fineness, and grammes per square metre is 20 ~ 2000g/m
2non-woven fabrics make, described bottom is 0.01 ~ 10 μm by fineness, and grammes per square metre is at 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor's one or more compounds of suppressing in film make.
Described three-layer composite structure sheet material is according to lines, and flower pattern, fabric construction cut-parts compacting forms, and the lines of the three-layer composite structure sheet material of compacting, flower pattern, leaves communication passage between fabric construction cut-parts.
Oxygenation air in described step (3) is in every litres of air, pass into 0.05 ~ 0.1 liter of oxygen.
Drying with dry actual conditions in described step (5) is temperature 50 ~ 80 DEG C, and humidity 50%, the time is 5 ~ 10h.
Grain roller when being pressed into three-layer composite structure sheet material, according to certain lines, flower pattern, apparel construction cut-parts or the cut-parts of bedclothes structure are suppressed, and the three-layer composite structure sheet material with hydrophobic permeable function has decorative pattern, in decorative pattern, are interconnected between decorative pattern; The three-layer composite structure sheet material of hydrophobic permeable function has lines, in lines, is interconnected between lines; The three-layer composite structure sheet material with hydrophobic permeable function has apparel construction or bedclothes structure, in apparel construction, be interconnected between apparel construction, apparel construction cut-parts can be upper coat-sleeve sheets, the cut-parts such as anter, rear panel, collar, pocket, also can be the cut-parts such as trousers sheet, waist panel, trouser pocket sheet, can also be the cut-parts such as cap, scarf, gloves; In bedclothes structure, be interconnected between bedclothes structure, the cut-parts of bedclothes structure can be the structure cut-parts such as sheet, quilt cover, Pillow cases.
Beneficial effect of the present invention: the present invention utilizes three layers of composite sheet super large oxygen supply area design, timing concentration sectional oxygen supply, the continuous method raising bacteria cellulose output combined for aerobe fermentation automatic control equipment and standing for fermentation technique, reduce costs, simultaneously in conjunction with fruit juice substratum bacteria cellulose clothing cutting plate or the direct technology of preparing of bedclothes cut-parts, solve bacteria cellulose clothes or the Production Flow Chart of bacteria cellulose bedclothes is long, cannot process and industrialized defect, the bacteria cellulose clothing cutting plate obtained or bedclothes cut-parts have good permeability, high-tensile and splendid proterties maintain ability.
Embodiment
Embodiment 1
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: be benchmark with water, adds the component of following masses mark: glucose 7%, yeast extract paste 0.5%, K
2hPO
43H
2o0.15%, MgSO
47H
2o1.5% and dehydrated alcohol, the volume of dehydrated alcohol is 2% of water, under the condition of 121 DEG C, sterilizing 30min; The preparation of fruit juice fermentation substratum: be benchmark with Sucus Cocois, adds 7g yeast extract paste in often liter of Sucus Cocois;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under 30 DEG C of conditions with 60r/min, fully vibrate, make bacterium liquid even, cultivate 24h; In the airtight pond of 7mL acetobacter xylinum access 100mL fermention medium;
(3) be 0.01 μm by fineness, grammes per square metre is 1g/m
2pP non-woven fabrics do surface layer, fineness is 2 μm of fineness, and grammes per square metre is 20g/m
2non-woven fabrics do sandwich layer, be 0.01 μm by fineness, grammes per square metre is at 1g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool twill lines, two anti-S blesses flower pattern, jacket sleeve, anter, rear panel, the apparel construction such as pocket and collar, lines, flower pattern, apparel construction, communication passage is left between cut-parts, above structure flower pattern sheet material forms apparel construction cut-parts, apparel construction cut-parts being dipped vertically into is equipped with in the airtight pond 3 of acetobacter xylinum fermention medium, under airtight pond temperature is the condition of 30 DEG C, by intake ducting at interval of 10min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, pass into 0.05 liter of oxygen, air is carried out in apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, at 30 DEG C of quiescent culture 7d, acetobacter xylinum is at apparel construction cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) the bacteria cellulose clothing cutting plate wet film of two cover same models can be separated after having fermented from apparel construction cut-parts, and rinse with water until except striping surface medium and impurity;
(5) step (4) being rinsed the bacteria cellulose clothing cutting plate wet film 0.1mol/L sodium hydroxide solution well boils to 80 DEG C, continue to boil 20min, removing thalline and remaining medium, then running water, at 50 DEG C, under the condition of humidity 50%, dry 5h, after drying, obtain bacteria cellulose clothing cutting plate.
Embodiment 2
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: be benchmark with water, adds the component of following masses mark: glucose 6%, yeast extract paste 0.1%, K
2hPO
43H
2o0.1% and MgSO
47H
2o1%, then add dehydrated alcohol, the volume of dehydrated alcohol is 1% of water, under the condition of 115 DEG C, and sterilizing 25min; The preparation of fruit juice fermentation substratum: be benchmark with Sucus Mali pumilae, adds 8g yeast extract paste in often liter of Sucus Mali pumilae;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under 25 DEG C of conditions with 70r/min, fully vibrate, make bacterium liquid even, cultivate 20h, in the airtight pond of 8mL acetobacter xylinum access 100mL fermention medium;
(3) be 10 μm by fineness, grammes per square metre is 30g/m
2pE non-woven fabrics do surface layer, fineness is 2000 μm, and grammes per square metre is 2000g/m
2non-woven fabrics do sandwich layer, be 10 μm by fineness, grammes per square metre is at 30g/m
2pE non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool plain weave lines, pine needle flower pattern, trousers sheet, want sheet, and the apparel construction such as trouser pocket sheet, lines, flower pattern, apparel construction, communication passage is left between cut-parts, above structure flower pattern sheet material forms apparel construction cut-parts, apparel construction cut-parts being dipped vertically into is equipped with in the airtight pond of acetobacter xylinum fermention medium, under airtight pond temperature is the condition of 37 DEG C, by intake ducting at interval of 30min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, pass into 0.1 liter of oxygen, air is carried out in apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, at 37 DEG C of quiescent culture 8d, acetobacter xylinum is at apparel construction cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) the bacteria cellulose clothing cutting plate wet film of two cover same models can be separated after having fermented from apparel construction cut-parts, and rinse with water until except striping surface medium and impurity;
(5) step (4) being rinsed the bacteria cellulose clothing cutting plate wet film 0.1mol/L sodium hydroxide solution well boils to 80 DEG C, continue to boil 20min, removing thalline and remaining medium, then running water, at 80 DEG C, under the condition of humidity 50%, dry 10h, after drying, obtain bacteria cellulose clothing cutting plate.
Embodiment 3
The working method of the fruit juice substratum bacteria cellulose clothing cutting plate of the present embodiment: comprise the following steps:
(1) preparation of seed culture medium: be benchmark with water, adds the component of following masses mark: glucose 10%, yeast extract paste 1%, K
2hPO
43H
2o0.5% and MgSO
47H
2o3%, then add dehydrated alcohol, dehydrated alcohol volume is 3% of water, under the condition of 125 DEG C, and sterilizing 40min; The preparation of fruit juice fermentation substratum: be benchmark with pineapple juice, adds 9g yeast extract paste in often liter of pineapple juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring activation, under 35 DEG C of conditions with 50r/min, fully vibrate, make bacterium liquid even, cultivate 30h, in the airtight pond of 9mL acetobacter xylinum access 100mL fermention medium;
(3) be 6 μm by fineness, grammes per square metre is 25g/m
2pS non-woven fabrics do surface layer, fineness is 200 μm, and grammes per square metre is 200g/m
2non-woven fabrics do sandwich layer, be 6 μm by fineness, grammes per square metre is at 25g/m
2pS non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool twill lines, two anti-S blesses flower pattern, the structure cut-parts such as sheet size, lines, flower pattern, sheet structure, communication passage is left between cut-parts, above structure flower pattern sheet material forms the cut-parts of sheet structure, the cut-parts of sheet structure being dipped vertically into is equipped with in the airtight pond of acetobacter xylinum fermention medium, under airtight pond temperature is the condition of 35 DEG C, by intake ducting at interval of 20min by air, oxygenation air and oxygen continuous circulation inject apparel construction cut-parts, oxygenation air is in every litres of air, pass into 0.08 liter of oxygen, air is carried out in apparel construction cut-parts surface in airtight pond, aerobic air and total oxygen continous way bacteria cellulose are cultivated, at 35 DEG C of quiescent culture 9d, acetobacter xylinum is at sheet structure cut-parts outside surface biosynthesizing bacteria cellulose clothing cutting plate wet film,
(4) the bacteria cellulose clothing cutting plate wet film of two cover same models can be separated after having fermented from the cut-parts of sheet structure, and rinse with water until except striping surface medium and impurity;
(5) step (4) being rinsed the bacteria cellulose sheet cut-parts wet film 0.1mol/L sodium hydroxide solution well boils to 80 DEG C, continue to boil 20min, removing thalline and remaining medium, then running water, at 65 DEG C, under the condition of humidity 50%, dry 8h, after drying, obtain the cut-parts of bacteria cellulose sheet.
Embodiment 4
A kind of working method of fruit juice substratum bacteria cellulose clothing cutting plate: be 1 μm by fineness, grammes per square metre is at 10g/m
2pP non-woven fabrics do surface layer, fineness is 20 μm of fineness, and grammes per square metre is that the non-woven fabrics of 200g/ every square does sandwich layer, and by fineness at 1 μm, grammes per square metre is at 10g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool twill lines, two anti-S blesses flower pattern, the apparel constructions such as jacket sleeve, anter, rear panel, pocket and collar, lines, flower pattern, leaves communication passage between apparel construction, cut-parts, and above structure flower pattern sheet material forms apparel construction cut-parts.By above apparel construction cut-parts according to certain arrangement regulation vertical out-fall in an airtight pond 3 of culturing bacterium Mierocrystalline cellulose, in apparel construction cut-parts, air, oxygenation air is injected through intake ducting, oxygen, meet the cellulosic growth of apparel construction cut-parts surface bacteria, carry out apparel construction cut-parts surface bacteria Mierocrystalline cellulose cultivate adding in airtight pond containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 7%(v/v) inoculum size.At apparel construction cut-parts outside surface biosynthesizing bacteria cellulose film, at 30 DEG C of constant temperature quiescent culture, through fermentation in 7 days, after having fermented, apparel construction cut-parts surface growth goes out bacteria cellulose film, the bacteria cellulose clothing cutting plate of two cover same models can be separated from apparel construction cut-parts, the bacteria cellulose clothing cutting plate wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose clothing cutting plate wet film is dried with drying plant respectively, make through sewing tech and sew on clothing.
Embodiment 5
A kind of working method of fruit juice substratum bacteria cellulose clothing cutting plate: be 5 μm by fineness, grammes per square metre is at 15g/m
2pP non-woven fabrics do surface layer, fineness is 20 μm of fineness, and grammes per square metre is 1000g/m
2non-woven fabrics do sandwich layer, by fineness at 5 μm, grammes per square metre is at 15g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool plain weave lines, pine needle flower pattern, trousers sheet, wants the apparel construction such as sheet and trouser pocket sheet, lines, flower pattern, leaves communication passage between apparel construction, cut-parts, and above structure flower pattern sheet material forms apparel construction cut-parts.By above apparel construction cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in apparel construction cut-parts, air, oxygenation air is injected through admission passage, oxygen, meet the cellulosic growth of apparel construction cut-parts surface bacteria, carry out apparel construction cut-parts surface bacteria Mierocrystalline cellulose cultivate adding in airtight pond containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 8%(v/v) inoculum size.At apparel construction cut-parts outside surface biosynthesizing bacteria cellulose film, at 30 DEG C of constant temperature quiescent culture, through fermentation in 8 days, after having fermented, apparel construction cut-parts surface growth goes out bacteria cellulose film, the bacteria cellulose clothing cutting plate of two cover same models can be separated from apparel construction cut-parts, the bacteria cellulose clothing cutting plate wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose clothing cutting plate wet film is dried with drying plant respectively, seam trousers are made through sewing tech.
Embodiment 6
A kind of working method of fruit juice substratum bacteria cellulose clothing cutting plate: be 10 μm by fineness, grammes per square metre is at 30g/m
2pP non-woven fabrics do surface layer, fineness is 1000 μm of fineness, and grammes per square metre is 2000g/m
2non-woven fabrics do sandwich layer, by fineness at 10 μm, grammes per square metre is at 30g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool satin weave lines, feather flower pattern, glove structure cut-parts, lines, flower pattern, leaves communication passage between glove structure, cut-parts, and above structure flower pattern sheet material forms glove structure cut-parts.By above glove structure cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in glove structure cut-parts, air, oxygenation air is injected through admission passage, oxygen, meet the growth taking glove structure cut-parts bacteria cellulose, carry out glove structure cut-parts bacteria cellulose cultivate adding in airtight pond containing the substratum of acetobacter xylinum, wherein bacterial strain M438 fermention medium is 9%(v/v) inoculum size.At glove structure cut-parts outside surface biosynthesizing bacteria cellulose film, at 30 DEG C of constant temperature quiescent culture, through fermentation in 9 days, after having fermented, glove structure cut-parts surface growth goes out bacteria cellulose film, the bacteria cellulose clothing cutting plate of two cover same models can be separated from glove structure cut-parts, the bacteria cellulose gloves cut-parts wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose gloves cut-parts wet film is dried with drying plant respectively, seam gloves are made through sewing tech.
Embodiment 7
The working method of a kind of fruit juice substratum bacteria cellulose bedclothes cut-parts: be 1 μm by fineness, grammes per square metre is at 10g/m
2pP non-woven fabrics do surface layer, fineness is 20 μm of fineness, and grammes per square metre is 200g/m
2non-woven fabrics do sandwich layer, by fineness at 1 μm, grammes per square metre is at 10g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool twill lines, two anti-S blesses flower pattern, the structure cut-parts such as sheet size, lines, flower pattern, sheet structure, communication passage is left between cut-parts, above structure flower pattern sheet material forms the cut-parts of sheet structure, by the cut-parts of above sheet structure according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in the cut-parts of sheet structure, air is injected through admission passage, oxygenation air, oxygen, meet the cellulosic growth of sheet structure cut-parts surface bacteria, substratum containing bacterial strain M438 is added in airtight pond and carries out the cultivation of sheet structure cut-parts surface bacteria Mierocrystalline cellulose, wherein bacterial strain M438 fermention medium is 7%(v/v) inoculum size.At sheet structure cut-parts outside surface biosynthesizing bacteria cellulose film, at 30 DEG C of constant temperature quiescent culture, through fermentation in 7 days, after having fermented, sheet structure cut-parts surface growth goes out bacteria cellulose film, the bacteria cellulose sheet cut-parts of two cover same models can be separated from the cut-parts of sheet structure, the bacteria cellulose sheet cut-parts wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose sheet cut-parts wet film is dried with drying plant respectively, sheet is made through sewing tech.
Embodiment 8
The working method of a kind of fruit juice substratum bacteria cellulose bedclothes cut-parts: be 5 μm by fineness, grammes per square metre is at 15g/m
2pP non-woven fabrics do surface layer, fineness is 1000 μm of fineness, and grammes per square metre is 100g/m
2non-woven fabrics do sandwich layer, by fineness at 5 μm, grammes per square metre is at 15g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool plain weave lines, pine needle flower pattern, the structures such as pillowcase, lines, flower pattern, leaves communication passage between pillowcase structure, cut-parts, and above structure flower pattern sheet material forms the cut-parts of pillowcase structure.By the cut-parts of above pillowcase structure according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, in the cut-parts of pillowcase structure, air, oxygenation air is injected through admission passage, oxygen, meet the cellulosic growth of pillowcase structure cut-parts surface bacteria, carry out pillowcase structure cut-parts surface bacteria Mierocrystalline cellulose cultivate adding in airtight pond containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 8%(v/v) inoculum size.At pillowcase structure cut-parts outside surface biosynthesizing bacteria cellulose film, at 35 DEG C of constant temperature quiescent culture, through fermentation in 8 days, after having fermented, pillowcase structure cut-parts surface growth goes out bacteria cellulose film, the bacteria cellulose pillowcase cut-parts of two panels (i.e. a pillowcase) same model can be separated from the cut-parts of pillowcase structure, the bacteria cellulose pillowcase cut-parts wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose pillowcase cut-parts wet film is dried with drying plant respectively, pillowcase is made through sewing tech.
Embodiment 9
The working method of a kind of fruit juice substratum bacteria cellulose bedclothes cut-parts: be 10 μm by fineness, grammes per square metre is at 30g/m
2pP non-woven fabrics do surface layer, fineness is 2000 μm of fineness, and grammes per square metre is 2000g/m
2non-woven fabrics do sandwich layer, by fineness at 10 μm, grammes per square metre is at 30g/m
2pP non-woven fabrics do bottom, three layers of flower pattern sheet material are pressed into through grain roller, according to UNICOM's structure design flower pattern sheet material, flower pattern sheet material tool satin weave lines, feather flower pattern, by cover structure cut-parts, lines, flower pattern, is left communication passage by between cover structure, cut-parts, and above structure flower pattern sheet material is formed by cover structure cut-parts.Will above by cover structure cut-parts according to certain arrangement regulation vertical out-fall in a cellulosic airtight pond of culturing bacterium, through admission passage to being injected air, oxygenation air in cover structure cut-parts, oxygen, meet by the growth of cover structure cut-parts bacteria cellulose, undertaken being cultivated by cover structure cut-parts bacteria cellulose by adding in airtight pond containing the substratum of bacterial strain M438, wherein bacterial strain M438 fermention medium is 9%(v/v) inoculum size.By cover structure cut-parts outside surface biosynthesizing bacteria cellulose film, at 37 DEG C of constant temperature quiescent culture, through fermentation in 9 days, after having fermented, bacteria cellulose film is gone out by cover structure cut-parts surface growth, from being separated the bacteria cellulose quilt cover cut-parts of two panels (i.e. a quilt cover) same model cover structure cut-parts, the bacteria cellulose gloves cut-parts wet film water separated repeatedly rinses, except striping surface medium and impurity, boil with 0.1mol/L sodium hydroxide solution, boil to temperature be 80 DEG C, time is 20min, remove the thalline in liquid film and remaining medium, with running water, be 7 through distilled water flushing to pH, bacteria cellulose quilt cover cut-parts wet film is dried with drying plant respectively, quilt cover is made through sewing tech.
Claims (5)
1. a working method for fruit juice substratum bacteria cellulose cloth cut-parts, is characterized in that comprising the following steps: the preparation of (1) seed culture medium: be benchmark with water, adds the component of following masses mark: glucose 6 ~ 10%, yeast extract paste 0.1 ~ 1%, K
2hPO
43H
2o0.1 ~ 0.5% and MgSO
47H
2o1 ~ 3% is benchmark with water, adds the dehydrated alcohol that volume fraction is 1 ~ 3%, under the condition of 115 ~ 125 DEG C, and sterilizing 25 ~ 40min; The preparation of fruit juice fermentation substratum: be benchmark with fruit juice, adds the yeast extract paste of 7 ~ 9g in often liter of fruit juice;
(2) spawn culture: by the acetobacter xylinum inclined-plane seed access seed culture medium of a ring or the activation of two rings, under the condition of 25 ~ 35 DEG C and 50 ~ 70r/min, fully vibrates, makes bacterium liquid even, cultivation 20 ~ 30h; In the airtight pond of acetobacter xylinum access fermention medium, the inoculum size of described acetobacter xylinum is 7 ~ 9% of fermention medium volume;
(3) fabric construction cut-parts are dipped vertically into are equipped with in the airtight pond of acetobacter xylinum fermention medium, under the condition of airtight pond temperature 30 ~ 37 DEG C, at interval of 10 ~ 30min, air, oxygenation air and oxygen continuous circulation are injected fabric construction cut-parts by pipeline, fabric construction cut-parts surface is carried out air, aerobic air and total oxygen continous way bacteria cellulose and is cultivated in airtight pond, under the condition of 30 ~ 37 DEG C, cultivate 7 ~ 9d, acetobacter xylinum is at fabric construction cut-parts outside surface biosynthesizing bacteria cellulose cloth cut-parts wet film;
(4) the bacteria cellulose cloth cut-parts wet film of two cover same models can be separated after having fermented from fabric construction cut-parts, and rinse with water until except striping surface medium and impurity;
(5) step (4) being rinsed the bacteria cellulose cloth cut-parts wet film 0.1mol/L sodium hydroxide solution well boils to 80 DEG C, continue to boil 20min, removing thalline and remaining medium, then running water, obtain the cut-parts of bacteria cellulose cloth after oven dry, drying;
The three-layer composite structure sheet material that fabric construction cut-parts in described step (3) are suppressed through grain roller by surface layer, sandwich layer and bottom; Described three-layer composite structure sheet material according to lines, flower pattern, fabric construction cut-parts compacting forms, the lines of three-layer composite structure sheet material of compacting, and flower pattern, leaves communication passage between fabric construction cut-parts;
Oxygenation air in described step (3) is in every litres of air, pass into 0.05 ~ 0.1 liter of oxygen.
2. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: in described step (1), fruit juice fermentation substratum is any one in Sucus Cocois fermention medium, apple juice fermentation substratum, pear juice fermention medium, pineapple juice fermention medium or fermentation of watermelon juice substratum.
3. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: the fabric construction cut-parts in described step (3) are apparel construction cut-parts or the cut-parts of bedclothes structure.
4. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, it is characterized in that: described surface layer is 0.01 ~ 10 μm by fineness, grammes per square metre is 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor's one or more compounds of suppressing in film make, described sandwich layer is 2 ~ 2000 μm of fineness by fineness, and grammes per square metre is 20 ~ 2000g/m
2non-woven fabrics make, described bottom is 0.01 ~ 10 μm by fineness, and grammes per square metre is at 1 ~ 30g/m
2pP, PE, PS, PET, PA polymer non-woven fabrics or bachelor's one or more compounds of suppressing in film make.
5. the working method of fruit juice substratum bacteria cellulose cloth according to claim 1 cut-parts, is characterized in that: drying with dry actual conditions in described step (5) is temperature 50 ~ 80 DEG C, and humidity 50%, the time is 5 ~ 10h.
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| CN106099182B (en) * | 2016-08-05 | 2018-08-03 | 宁波高智科技咨询服务有限公司 | A kind of lithium battery bacterial cellulose gel method for preparing polymer electrolytes |
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| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
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| CN102392062A (en) * | 2011-11-18 | 2012-03-28 | 东华大学 | Method for preparing bacterial cellulose by using decayed fruits as raw materials |
| CN102533904A (en) * | 2012-01-17 | 2012-07-04 | 东华大学 | Method and device for preparing bacterial cellulose composite material quickly on large scale |
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