CN104630908B - A kind of method that volume branch Mucor DK1 bacterial strain and hydrogen peroxide combined production process prepare flaxen fiber - Google Patents

A kind of method that volume branch Mucor DK1 bacterial strain and hydrogen peroxide combined production process prepare flaxen fiber Download PDF

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CN104630908B
CN104630908B CN201510080723.1A CN201510080723A CN104630908B CN 104630908 B CN104630908 B CN 104630908B CN 201510080723 A CN201510080723 A CN 201510080723A CN 104630908 B CN104630908 B CN 104630908B
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hydrogen peroxide
bacterial strain
degumming
production process
flaxen fiber
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CN104630908A (en
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丁若垚
刘国亮
郁崇文
张兴群
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Donghua University
Yangcheng Institute of Technology
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Yangcheng Institute of Technology
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    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D01C1/04—Bacteriological retting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
    • D01C1/00—Treatment of vegetable material
    • D01C1/02—Treatment of vegetable material by chemical methods to obtain bast fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/785—Mucor

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Abstract

The present invention relates to a kind of method that volume branch Mucor DK1 bacterial strain prepares flaxen fiber with hydrogen peroxide combined production process, including: (1) cultivates bacterial strain；(2) crude enzyme liquid is prepared；(3) the crudefiber crop raw material after sterilizing is mixed by mass volume ratio 1:10～30 with the mixed liquor of crude enzyme liquid and hydrogen peroxide, regulation pH value, then it is placed in shaking table and processes, it is eventually adding NaOH, and rises high-temperature and carry out degumming, remove degumming liquid, rinse, when pH value is nature, stops rinsing, complete degumming.The strain growth cycle that the present invention uses is short, and bacterial classification is difficult to be contaminated, and processing cost is low, and de-alkali additives is gentle；Degumming method flow process is simple, and the time shortens, and energy consumption reduces, and blowdown flow rate reduces, and favorable environment is protected.

Description

A kind of method that volume branch Mucor DK1 bacterial strain and hydrogen peroxide combined production process prepare flaxen fiber
Technical field
The invention belongs to biological textile technology field, prepare with hydrogen peroxide combined production process particularly to one volume branch Mucor DK1 bacterial strain The method of flaxen fiber.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly has flax, ramie, hemp, bluish dogbane, jute, bluish dogbane, sisal hemp, any of several broadleaf plants Fiber crops, nettle and piemarker etc..Ramie is the distinctive hemp resource of China, is referred to as " China's grass ", and China is main ramie in the world Fiber crops producing country, ramie yield accounts for Gross World Product more than 90%；Flax fiber is commonly called as " fiber queen ", is the most ancient Textile fabric, the textile applications made is the most extensive.Hemp is also one of textile fabric of utilizing the earliest of the mankind, China hemp Yield occupies first place in the world.As green ecological fiber, flaxen fiber has moisture absorption, ventilative, antibacterial, mildew-resistant, uvioresistant, nothing The premium properties such as electrostatic, made textile has the characteristics such as ventilative nice and cool, antibacterial anticorrosion.National energy-saving reduce discharging policy and Under consumer's back to nature, the consumption idea of pursuit green promote, bast-fibre green processing technologies has become as field of textiles Study hotspot.
Flaxen fiber is of a great variety, and they are made up of the cellulose of different specific weight, hemicellulose, lignin, pectin and impurity, spin Knit in processing, it is necessary to through " degumming ", flax degumming, also known as flax roving boiling-off, i.e. removes pectin, hemicellulose and lignin Deng non-cellulosic material (being referred to as colloid), the fiber for light textile could be obtained, it is achieved its use value.Conventional microorganism Degumming be utilize microorganism with the colloid in flaxen fiber be its carbon element, nitrogen nutrition source characteristic, by the pectin in flaxen fiber, The substance decomposition such as hemicellulose, lignin is converted into simple lower-molecular substance, therefrom obtains the nutrition needed for vital movement own Material and heat energy, thus complete the scouring processes of flaxen fiber.By gathering bacterium sample, then carry out enrichment culture, isolated and purified, Stalwartness can be turned out under suitable culture medium and condition of culture and have the bacterial classification of stronger vitality, being then seeded into needing degumming Degumming is carried out on raw ramie.
The chemical Degumming method commonly used in industry need to produce one ton of degummed ramie typically require 500-700 ton through 4-6 washing Water, and work in producing discontinuous, labour intensity big, the degumming tech time is long, energy consumption is high, and degumming quality affects outside fabric See.Less adapt to top grade, high-count cloth needs.In order to remove more colloid, improve degumming efficiency, facilitate later process to add Work, industry have selected chemical method and bacterium combined to flax kiering, and these method Combined Treatment fiber crops are the processing works producing good fiber quality Skill, bacterial classification during it selects and adds the key that the technique of hydrogen peroxide is degumming.
Summary of the invention
It is fine with hydrogen peroxide combined production process preparation fiber crops that the technical problem to be solved is to provide a kind of volume branch Mucor DK1 bacterial strain The method of dimension, the strain growth cycle that the method uses is short, and bacterial classification is difficult to be contaminated, and processing cost is low, and de-alkali additives is gentle； Degumming method flow process is simple, and the time shortens, and energy consumption reduces, and blowdown flow rate reduces, and favorable environment is protected.
The method that a kind of volume branch Mucor DK1 bacterial strain of the present invention and hydrogen peroxide combined production process prepare flaxen fiber, including:
(1) volume branch Mucor DK1 bacterial strain is stored in PDA medium culture, adds frozen buffer solution；Cultivate via PDA inclined-plane Base is transferred equipped with in the triangular flask of basal liquid medium, accesses bacterium one ring of gained, 25～30 DEG C of isothermal vibrations in shaking table After cultivating 3～4d, culture medium inoculated enters flax fermentation medium, and Shaking culture obtains zymocyte liquid, and zymocyte liquid is through being filtrated to get Mycelium, mycelium sterilized water is cleaned and is broken up, is collected in subsequently in sterilized water and makes hyphal suspension, save backup；
(2) in basal liquid medium, it is separately added into the natural substrate of 1～2%w/v and the CuSO of variable concentrations4·5H2O is as product Enzyme culture medium, loads culture medium in triangular flask, accesses above-mentioned hyphal suspension, 25～30 DEG C of isothermal vibrations in shaking table Cultivate, timing extraction zymotic fluid in incubation, take supernatant after being centrifuged and be crude enzyme liquid；
(3) the crudefiber crop raw material after sterilizing is mixed by mass volume ratio 1:10～30 with the mixed liquor of above-mentioned crude enzyme liquid and hydrogen peroxide, regulation PH value, is then placed in shaking table and processes, and is eventually adding NaOH, and rises high-temperature and carry out degumming, removes degumming liquid, Rinse, when pH value is nature, stops rinsing, complete degumming.
The ITS sequence of the volume branch Mucor DK1 bacterial strain in described step (1) is as shown in SEQ ID NO:1.
According to ITS order-checking Blast and cluster analysis result, this bacterium and Mucor circinelloides similitude are 99%, try in conjunction with Physiology and biochemistry Test result and microscopy analysis, name this bacterial strain for volume branch Mucor (Mucor circinelloides) DK1 bacterial strain.In ITS order-checking, The forward primer used is: ITS1 (5-TCCGTAGGTGAACCTGCGG-3 '), reverse primer are ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 '), it is international primer, the raw work biotechnology service in Shanghai has Limit company synthesizes.
Volume branch Mucor (Mucor circinelloides) DK1 bacterial strain of the present invention, depositary institution's title: in CGMCC State's Microbiological Culture Collection administration committee common micro-organisms center, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Number Institute of Microorganism, Academia Sinica, preserving number is CGMCC NO.10143, and preservation date is on December 15th, 2014. Suggestion Classification And Nomenclature is: volume branch Mucor (Mucor circinelloides).
The formula of the every 100ml of frozen buffer solution in described step (1) is: potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.02g, Sodium citrate 0.06g, bitter salt 0.03g, glycerine 12ml, add water and be settled to 100ml, prepares.
Consisting of of basal liquid medium in described step (1): potato 200g, glucose 20g, distilled water 1000mL, PH is natural；Consisting of of flax fermentation medium: every 1000ml culture medium includes waste of flax 200g, wheat bran 50g, brewer's wort 50ml and water 950ml.
Storage temperature in described step (1) is 40 DEG C.
Natural substrate in described step (2) is tealeaves, straw, wheat straw, peanut shell, dregs of beans, bagasse, tangerine peel, cornstalk Or wheat drum；CuSO4·5H2The concentration range of O is 0-4mM.
Consisting of of culture medium in described step (2): NaNO32.5g, KH2PO41g, CaCl2·6H2O 0.1g, MgSO40.3g, NaCl 0.1g, FeCl30.01g, rape straw 0.5g, distilled water 1000mL, pH watery hydrochloric acid is adjusted to 6.0-7.0。
Crudefiber crop raw material in described step (3) is former stem, fiber, yarn or the fabric of flax, ramie, jute, bluish dogbane or bamboo.
Crude enzyme liquid and the volume ratio of hydrogen peroxide in described step (3) are 1:10；Described regulation pH value is to 4.0-7.0.
Shaking table in described step (3) processes technological parameter: temperature 10-60 DEG C, rotating speed 10-300rpm, processes the time 10min-5d；Degumming tech parameter is: usually time 10min-24h, degumming temperature 50-80 DEG C, programming rate 0.1-10 DEG C/min, Sodium hydroxide concentration is the 0.5%-30% of crudefiber crop material quality.
Beneficial effect
(1) the strain growth cycle that the present invention uses is short, and bacterial classification is difficult to be contaminated, and processing cost is low, and de-alkali additives is gentle, antipollution Ability is strong, and heat resistance is good, non-environmental-pollution, and after process, fiber quality is good；This bacterial strain have to flax, ramie, jute, The non-cellulosic material of the crudefiber crop raw material such as bluish dogbane and bamboo has the ability of degraded, can apply to bast fiber surface modification.
(2) during utilizing volume branch Mucor DK1 bacterial classification produced enzyme liquid to process crudefiber crop raw material, environmentally friendly hydrogen peroxide is used Auxiliary enzymes processes, and utilizes hydrogen peroxide reduction in acid condition, reduces the hydrolysis in acid condition of crudefiber crop raw material； Meanwhile, utilize hydrogen peroxide oxidisability in the basic conditions and Bleachability, the most right under the common effect of enzyme and NaOH Crudefiber crop raw material carries out degumming process.By pre-treatment, the degumming in crudefiber crop raw material tradition degumming tech, inactivate, the letter of the operation such as bleaching Turn to an operation.Degumming method flow process provided by the present invention is simple, and the time shortens, and energy consumption reduces, and blowdown flow rate reduces, and has Profit environmental protection.
Accompanying drawing explanation
Fig. 1 and Fig. 2 is bacterial strain microscope stained photographs of the present invention (oil mirror).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments be merely to illustrate the present invention and not For limiting the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, those skilled in the art can To make various changes or modifications the present invention, these equivalent form of values fall within the application appended claims limited range equally.
Embodiment 1
The method that the present embodiment record volume branch Mucor DK1 prepares degummase liquid:
(1) volume branch Mucor DK1 bacterial strain being stored in PDA culture medium, 30 DEG C of 200rpm cultivate 24 hours, add frozen slow Rush liquid；The 300mL triangle of the 50mL basal liquid medium after transferring equipped with sterilizing cooling via PDA slant medium In Ping, access bacterium one ring of gained, and 150rpm in shaking table, after 28 DEG C of isothermal vibrations cultivate 3d, 50ml shaking flask is trained After Yanging, culture medium inoculated enters flax fermentation medium 5L, and under the conditions of rotating speed 200r/min, temperature 30 DEG C, Shaking culture 24 is little Time obtain zymocyte liquid, fermentation liquor sterile gauze filters, and mycelium sterilized water is cleaned and smashed in cup at a high speed at Waring and beats Dissipate 10s, be collected in sterilized water (with zymotic fluid equal-volume) and make hyphal suspension, save backup at 40 DEG C.
The formula of the every 100ml of frozen buffer solution is: potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.02g, sodium citrate 0.06g, Bitter salt 0.03g, glycerine 12ml, add water and be settled to 100ml, prepares.
Consisting of of basal liquid medium: potato 200g, glucose 20g, distilled water 1000mL, pH are natural.
Consisting of of flax fermentation medium: every 1000ml culture medium includes waste of flax 200g, wheat bran 50g, brewer's wort 50ml With water 950ml.
(2) be separately added in basal liquid medium 1% (w/v) natural substrate (tealeaves, straw, wheat straw, peanut shell, Dregs of beans, bagasse, tangerine peel, cornstalk or wheat drum) and the CuSO of variable concentrations4·5H2O (0-4mM) is culture medium.300 ML triangular flask loads culture medium 80mL, accesses 20mL hyphal suspension, to be not added with natural substrate or copper sulphate Sample be comparison, 150rpm in shaking table, 28 DEG C of isothermal vibrations are cultivated.Timing extraction zymotic fluid in incubation, centrifugal Rear supernatant is crude enzyme liquid, the mensuration lived for enzyme.
Consisting of of culture medium: NaNO32.5g, KH2PO41g, CaCl2·6H2O 0.1g, MgSO40.3g, NaCl 0.1g, FeCl30.01g, rape straw 0.5g, distilled water 1000mL, pH watery hydrochloric acid is adjusted to 6.0-7.0.
Embodiment 2
Being prepared laccase by volume branch Mucor DK1 bacterial strain, combine the technique for flax straw degumming with hydrogen peroxide as follows:
5g flax straw is placed in 250ml conical flask, presses 1:20 (w/v) with flax straw with crude enzyme liquid and hydrogen peroxide mixed liquor Being sub-packed in conical flask, liquid amount is 100ml, and mixed liquor pH value is 6.0, and flax straw and hydrogen peroxide mass ratio are 1:5, with Crude enzyme liquid mass ratio is 1:15.At 40 DEG C, difference degumming 1 day, 2 days and 4 days in 200rpm shaking table, the time is to the setting time Rear addition 10% NaOH, design temperature 75 DEG C, 1 DEG C/min of programming rate, the time is 5 hours, and usually time arrives, and removes Remove degumming liquid, rinse for several times with running water, to remove the bacterium on flax straw and non-cellulosic material, complete degumming.
By to flax degumming it has been observed that after degumming, the most bundles of flax is dispersed into threadiness, the obvious retrogradation of zymotic fluid； After hydrogen peroxide oxidation degumming, flax whiteness increases, and degumming rate increases.
Embodiment 3
The technique for ramie yarn and thread is combined with hydrogen peroxide as follows by volume branch Mucor DK1:
5g ramie sterilizing yarn (ramie rove or ramie spinning) is inserted 250ml conical flask, with ramie yarn and thread and crude enzyme liquid and Hydrogen peroxide mixed liquor is sub-packed in conical flask by 1:20 (w/v), and liquid amount is 100ml, and mixed liquor pH value is 7.0, ramie Yarn and hydrogen peroxide mass ratio are 1:1, are 1:19 with crude enzyme liquid mass ratio.At 10 DEG C, difference degumming 10min in 10rpm shaking table, Adding 0.5% NaOH, design temperature 50 DEG C, 10 DEG C/min of programming rate after time to setting time, the time is 10min, Usually time then, removes degumming liquid, rinses for several times with running water, to remove the bacterium on ramie yarn and thread and non-cellulosic material, Complete degumming.
By ramie yarn and thread degumming it has been observed that after degumming, ramie yarn and thread pliability improves, whiteness increases, and yarn strength is not sent out Raw significant change.
Embodiment 4
The technique for bamboo degumming is combined with hydrogen peroxide as follows by volume branch Mucor DK1:
Being sub-packed in conical flask by 1:20 (w/v) with crude enzyme liquid and hydrogen peroxide mixed liquor with bamboo, liquid amount is 100ml, mixing Liquid pH value is 4.0, and bamboo and hydrogen peroxide mass ratio are 1:19, are 1:1 with crude enzyme liquid mass ratio.At 60 DEG C, in 300rpm shaking table Respectively degumming 5d, adds 30% NaOH after the time to setting time, design temperature 80 DEG C, 0.1 DEG C/min of programming rate, Time is 24h, and usually time then, removes degumming liquid, rinses for several times with running water, to remove the bacterium on bamboo and non-fiber Element material, completes degumming.
By to degumming bamboo it has been observed that after degumming bamboo decentralization significantly improve, pliability improve.
Embodiment 5
The technique for jute degumming is combined with hydrogen peroxide as follows by volume branch Mucor DK1:
Being sub-packed in conical flask by 1:20 (w/v) with crude enzyme liquid and hydrogen peroxide mixed liquor with jute, liquid amount is 100ml, mixed Closing liquid pH value is 5.0, and jute and hydrogen peroxide mass ratio are 1:10, are 1:10 with crude enzyme liquid mass ratio.At 50 DEG C, 250rpm Respectively degumming 4d in shaking table, adds 20% NaOH after the time to setting time, design temperature 70 DEG C, 2 DEG C/min of programming rate, Time is 12h, and usually time then, removes degumming liquid, rinses for several times with running water, to remove the bacterium on jute and non-fibre Dimension element material, completes degumming.
By to degumming jute it has been observed that after degumming jute whiteness, pliability significantly improve, prodding and itching feeling significantly lowers.
Embodiment 6
The technique for bluish dogbane fabric degumming is combined with hydrogen peroxide as follows by volume branch Mucor DK1:
Being sub-packed in conical flask by 1:20 with crude enzyme liquid and hydrogen peroxide mixed liquor with bluish dogbane fabric, liquid amount is 100ml, mixing Liquid pH value is 5.5, and bluish dogbane fabric and hydrogen peroxide mass ratio are 1:15, are 1:5 with crude enzyme liquid mass ratio.At 55 DEG C, 150rpm Respectively degumming 1d in shaking table, adds 5% NaOH after the time to setting time, design temperature 60 DEG C, 5 DEG C/min of programming rate, Time is 2h, and usually time then, removes degumming liquid, rinses for several times with running water, to remove the bacterium on bluish dogbane fabric and non- Cellulosic material, completes degumming.
By to degumming bluish dogbane fabric it has been observed that after degumming bluish dogbane fabric whiteness increase, pliability improve, prodding and itching feeling lower.

Claims (9)

1. roll up the method that a Mucor DK1 bacterial strain prepares flaxen fiber with hydrogen peroxide combined production process, including:
(1) volume branch Mucor DK1 bacterial strain is stored in PDA medium culture, adds frozen buffer solution；Cultivate via PDA inclined-plane Base is transferred equipped with in the triangular flask of basal liquid medium, accesses bacterium one ring of gained, 25～30 DEG C of isothermal vibrations in shaking table After cultivating 3～4d, culture medium inoculated enters flax fermentation medium, and Shaking culture obtains zymocyte liquid, and zymocyte liquid is through being filtrated to get Mycelium, mycelium sterilized water is cleaned and is broken up, is collected in subsequently in sterilized water and makes hyphal suspension, save backup；
(2) in basal liquid medium, it is separately added into the natural substrate of 1～2%w/v and the CuSO of variable concentrations4·5H2O is as product Enzyme culture medium, loads culture medium in triangular flask, accesses above-mentioned hyphal suspension, 25～30 DEG C of isothermal vibrations in shaking table Cultivate, timing extraction zymotic fluid in incubation, take supernatant after being centrifuged and be crude enzyme liquid；
(3) the crudefiber crop raw material after sterilizing is mixed by mass volume ratio 1:10～30 with the mixed liquor of above-mentioned crude enzyme liquid and hydrogen peroxide, regulation PH value, is then placed in shaking table and processes, and is eventually adding NaOH, and rises high-temperature and carry out degumming, removes degumming liquid, Rinse, when pH value is nature, stops rinsing, complete degumming.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: the ITS sequence of the volume branch Mucor DK1 bacterial strain in described step (1) is as shown in SEQ ID NO:1.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: the formula of the every 100ml of frozen buffer solution in described step (1) is: potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.02g, sodium citrate 0.06g, bitter salt 0.03g, glycerine 12ml, add water and be settled to 100ml, prepares.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: consisting of of the basal liquid medium in described step (1): potato 200g, glucose 20g, distilled water 1000mL, pH are natural；Consisting of of flax fermentation medium: every 1000ml culture medium includes waste of flax 200g, wheat bran 50g, Brewer's wort 50ml and water 950ml.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: the storage temperature in described step (1) is 40 DEG C.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its Be characterised by: the natural substrate in described step (2) be tealeaves, straw, wheat straw, peanut shell, dregs of beans, bagasse, tangerine peel, Cornstalk or wheat drum；CuSO4·5H2The concentration range of O is 0-4mM.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: the crudefiber crop raw material in described step (3) is the former stem of flax, ramie, jute or bluish dogbane, fiber, yarn or knits Thing.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its It is characterised by: the volume ratio between crude enzyme liquid and hydrogen peroxide in described step (3) is 1:10；Described regulation pH value is to 4.0-7.0.
The method that a kind of volume branch Mucor DK1 bacterial strain the most according to claim 1 and hydrogen peroxide combined production process prepare flaxen fiber, its Being characterised by: the shaking table in described step (3) processes technological parameter and is: temperature 10-60 DEG C, rotating speed 10-300rpm processes Time 10min-5d；Degumming tech parameter is: usually time 10min-24h, degumming temperature 50-80 DEG C, programming rate 0.1-10 DEG C/min, sodium hydroxide concentration is the 0.5%-30% of crudefiber crop material quality.
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Publication number Priority date Publication date Assignee Title
JPH08199420A (en) * 1995-01-18 1996-08-06 Akio Onda Production of highly flexible vegetable fiber
CN1188513C (en) * 2002-04-12 2005-02-09 山东省纤维检验局 Prepn and use of enzyme prepn for use in hemp degluing industrial process
CN100355951C (en) * 2006-04-06 2007-12-19 浙江理工大学 High temperature-microbe combined hemp degumming method
CN101285211A (en) * 2008-06-05 2008-10-15 黑龙江省科学院亚麻综合利用研究所 Microorganism retting flax method for crudefiber crops
CN103276456A (en) * 2013-06-08 2013-09-04 太仓市芸芸化纤有限公司 Flax fiber degumming process
CN103484949A (en) * 2013-09-11 2014-01-01 昆山市万丰制衣有限责任公司 Degumming process for mulberry fibers

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