CN102409411B - Method for preparing fibrilia by using epicoccum nigrum DB3 strains - Google Patents
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Abstract
The invention relates to a method for preparing fibrilia by using epicoccum nigrum DB3 strains. The method comprises the following steps: (1) preserving the epicoccum nigrum DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.
Description
Technical field
The invention belongs to fungi and prepare the flaxen fiber field, particularly a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, jute, bluish dogbane, kendir, sisal hemp, abaca, nettle and piemarker etc.Flaxen fiber is the general designation of the fiber obtained from various numb plants, has moisture absorption, ventilative, heat radiation and the incomparable advantage of other natural fibers such as antibiotic.China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber of producing and goods thereof also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, are earned foreign exchange nearly 10,000,000,000 dollars except satisfying the demand of domestic market every year.Reduce discharging policy and human consumer's back to nature at national energy-saving, pursue under the green consumption idea promotion, bast-fibre green processing technology has become the study hotspot of field of textiles.
The chief component of flax, hemp, jute and bastose is Mierocrystalline cellulose, also has in addition the non-cellulose materials such as more hemicellulose, pectin and xylogen to be wrapped in the flaxen fiber surface.The non-cellulose materials such as these colloids, particularly xylogen are cementing in the fiber outside, so that raw ramie is firm sheet strip.So raw ramie must be removed the colloids such as xylogen by the process of coming unstuck together in spinning process, to satisfy spinning requirement.At present, the kiering method that bast industry is commonly used mainly is chemical process, namely utilizes strong acid and strong base that raw ramie or numb rove are processed.The chemical treatment technology energy consumption is large, and the waste water of the large and discharging of equipment loss can't recycle, and pollution problem is serious.
Summary of the invention
Technical problem to be solved by this invention provides a kind of epicoccum nigrum (Epicoccum nigrum) DB3 bacterial strain that utilizes and prepares the method for flaxen fiber, and this preparation technology is simple, is fit to the characteristics such as large-scale industrial production.
A kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber of the present invention comprises:
(1) epicoccum nigrum DB 3 strains is stored in potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Wherein frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10mL adds water and is settled to 100mL and makes; The prescription of potato dextrose agar is: potato 200g, glucose 20g, tap water 1000mL, natural pH value;
(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;
(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100mL constant volume;
(4) the numb raw material after will sterilizing mixes by bath raio with step (3) gained zymocyte liquid at 1: 20, at 30 ℃, processes in the 160-220rpm shaking table 1～4 day, removes fermented liquid, obtains flaxen fiber;
(5) above-mentioned flaxen fiber and come unglued liquid are mixed, under 70-100 ℃ of temperature, processed 1-8 hour, then washing, and method routinely oils, drying, namely obtaining can be for the fiber of weaving; Wherein flaxen fiber is 1 to the weight ratio of come unglued liquid: 8-1: 25, and the temperature of coming unstuck is 70-100 ℃, the time is 1-8 hour.
Numb raw material described in the step (4) is flax straw, linen thread and yarn, sodolin, hemp original stem, hemp yarn, hemp cloth, the former stem of jute, Jute thread and yarn, megila, the former stem of bluish dogbane, bluish dogbane yarn or bluish dogbane fabric.
Come unglued liquid described in the step (5) is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water; The weight ratio 1 of wherein peroxide degumming agent, sequestrant and hydrogen bond disrupting agent sum and the solution that comes unstuck: 8-1: 25.
Peroxide degumming agent described in the step (5) is hydrogen peroxide, Potassium Persulphate, Sodium Persulfate, ammonium persulphate, antihypo, SPC-D, percarbonic acid ammonium, potassium per(oxy)borate, Sodium peroxoborate or ammonium pertorate, and its consumption is the 0.25-8% of numb raw material weight.
Sequestrant described in the step (5) is sodium phosphate, tripoly phosphate sodium STPP, one or more of sodium-metaphosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, citric acid, disodium ethylene diamine tetraacetate, and its consumption is the 0.1-4% of numb raw material weight.
Hydrogen bond disrupting agent described in the step (5) is urea, and its consumption is the 0.05-2% of numb raw material weight.
Flaxen fiber described in the step (5) and come unglued liquid mix as 1: 10 ratio take weight ratio, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is hydrogen peroxide, and its consumption is 3% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 0.05% of numb raw material weight, and sequestrant is tripoly phosphate sodium STPP and disodium ethylene diamine tetraacetate, and its consumption is respectively 2% and 0.1% of numb raw material weight.
Flaxen fiber described in the step (5) and come unglued liquid mix as 1: 25 ratio take weight ratio, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is the percarbonic acid ammonium, and its consumption is 5% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 1% of numb raw material weight, and sequestrant is Sodium phosphate dibasic and SODIUM PHOSPHATE, MONOBASIC, and its consumption is 2% of numb raw material weight.
Flaxen fiber described in the step (5) and come unglued liquid mix as 1: 8 ratio take weight ratio, and the temperature of coming unstuck is 100 ℃, and the time is 8 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is potassium per(oxy)borate, and its consumption is 8% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 2% of numb raw material weight, and sequestrant is sodium-metaphosphate, disodium ethylene diamine tetraacetate and tripoly phosphate sodium STPP, and its consumption is 2% of numb raw material weight.
Flaxen fiber described in the step (5) and come unglued liquid mix as 1: 15 ratio take weight ratio, and the temperature of coming unstuck is 90 ℃, and the time is 2 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is ammonium persulphate, and its consumption is 2% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 0.05% of numb raw material weight, and sequestrant is tripoly phosphate sodium STPP and sodium phosphate, and its consumption is respectively 2% and 1% of numb raw material weight.
The preserving number of epicoccum nigrum (Epicoccum nigrum) the DB3 bacterial strain that the present invention adopts is CGMCC No.4687; Preservation day is on March 17th, 2011, and the Classification And Nomenclature of suggestion is epicoccum nigrum (Epicoccum nigrum), is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, the address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City.
According to 18sR dna sequencing Blast and cluster analysis result, this bacterium and epicoccum nigrum similarity are 99%, and in conjunction with physiological and biochemical test result and microscopy analysis, naming this bacterial strain is epicoccum nigrum (Epicoccum nigrum) DB3 bacterial strain.
The preparation method of the epicoccum nigrum DB 3 strains that the present invention adopts is as follows:
(1) sea grass of will rotting places the enrichment medium room temperature to leave standstill in the ratio of 1g: 20mL to cultivate 30 days, and then got the 0.1mL enrichment medium and coat in the isolation medium, and 30 ℃ leave standstill and cultivated 1～4 day, obtain wild-type flax biological treatment bacterial strain;
Wherein, the enrichment culture based formulas is: flax powder 5g, tap water 200mL, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH
4)
2SO
45g, K
2HPO
41g, MgSO
40.5g, KCl 0.5g, FeSO
40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃.The Flax Lignin nutritional medium, its component comprises: Flax Lignin 2g, NaNO
33g, K
2HPO
40.5g, MgSO
41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(3) from above-mentioned substratum, select the bacterial strain with lignin degrading ability.
Under the culture condition that makes up, this strain culturing condition is extensive, good heat resistance, and growth and breeding is fast, can effectively remove the colloids such as xylogen in flax, hemp and the jute-kenaf fibres, and enzyme is alive stable, bacterium, the equal nontoxicity of enzyme.This bacterial strain and culture systems can be directly used in flax retting.
The strain growth cycle of the present invention is short, and bacterial classification is difficult for contaminated, and processing cost is low, the mild condition of coming unstuck, and contamination resistance is strong, and resistance toheat is good, non-environmental-pollution, fiber quality is good after processing; This bacterial strain has the ability to phloem fiber lignin degradations such as flax, hemp, jute or bluish dogbanes, can be applied to the phloem fiber surface modification.
After bacterial classification pre-treatment or the processing, the friendly type superoxide of real-world environment carries out inactivation treatment, to substitute traditional boiling water inactivation technology, with water saving; Numb sample after the strong oxidizing property of utilizing simultaneously superoxide in the inactivation technology is processed bacterial classification carries out refining and bleaching, with further Optimization Technology, energy-saving and emission-reduction.
Compared with prior art, the present invention is applied to the deactivation of bacterial classification, refining and the bleaching process of flaxen fiber with environmentally friendly superoxide, and three operations are merged into an operation.Therefore the present invention has that technique is simple, Production Flow Chart short, is fit to the characteristics such as large-scale industrial production, and to carry out the Bio-Pretreatment that xylogen is removed before phloem fiber surface modification and the textile process under mass production conditions significant for exploring penicillium purpurogenum.
Beneficial effect
(1) bacterial strain of the present invention and culture systems, simultaneously high yield polygalacturonase and hemicellulase can be directly used in flax retting after testing, have the cycle of coming unstuck weak point, the fiber dispersion rate reaches 100%, and is respond well to flax retting, the efficient of coming unstuck reaches 95%, bacterial classification is difficult for contaminated, and processing cost is low, and fiber quality is good, the mild condition of coming unstuck, contamination resistance is strong, and resistance toheat is good, the advantages such as non-environmental-pollution;
(2) preparation method of the present invention has the characteristics such as simple, the suitable large-scale industrial production of technique.Utilize this system to carry out flax retting and have the usually time weak point, the flax fiber scatter coefficient reaches 100%, and degumming rate reaches more than 90%, and the degummed ramie quality is good.
Description of drawings
Fig. 1 is fungi microscope dyeing photo (oily mirror);
Fig. 2 is potato potato culture bacterium colony photo;
Fig. 3 is fungi treating processes photo;
Fig. 4 is flax fiber photo after processing.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
The screening of flax retting bacterial classification obtains
(1) from the rotten sea grass of East Sea Zhoushan sea area sampling, first sea grass and enrichment medium are left standstill in ratio room temperature in enrichment medium of 1g: 20mL and cultivated 30 days, then get the 0.1mL enrichment medium and coat isolation medium, 30 ℃ leave standstill cultivation 1～4 day, obtain wild-type flax biological treatment dominant strain;
Wherein, the enrichment culture based formulas is: flax powder 5g, tap water 200mL, pH nature; The separation and Culture based formulas is: flax powder 5g, (NH
4)
2SO
45g, K
2HPO
41g, MgSO
40.5g, KCl 0.5g, FeSO
40.01, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(2) with the inoculation that obtains in the step (1) in the Flax Lignin nutritional medium, cultivate 72h for 28 ℃.The Flax Lignin nutritional medium, its component comprises: Flax Lignin 2g, NaNO
33g, K
2HPO
40.5g, MgSO
41g, agar 20g, water 1000mL arrives 7-7.2 with NaOH with pH regulator;
(3) from above-mentioned substratum, select the bacterial strain with lignin degrading ability.
Embodiment 2
The preparation of zymocyte liquid:
(1) chain lattice spore DB2 bacterial strain is stored in potato dextrose agar, and 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10mL adds water and is settled to 100mL and makes;
(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;
(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100mL constant volume.
Embodiment 3
Flax retting technique is as follows:
The 5g flax straw is inserted the 250mL triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of flax straw and embodiment 2, liquid amount is 100mL, at 40 ℃, comes unstuck respectively in the 200rpm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Flax straw after the biological treatment is carried out inactivation treatment, its technique is: the flax straw after the biological treatment and come unglued liquid mix with 1: 10 ratio, and under 70 ℃ condition, processed 3 hours, wherein the consumption of superoxide hydrogen peroxide is 3% of flax straw weight, amount of urea is 0.05% of flax straw weight, the consumption of tripoly phosphate sodium STPP is 2% of flax straw weight, and the consumption of disodium ethylene diamine tetraacetate is 0.1% of flax straw weight.2130 weaving flax fiber can making after coming unstuck.
Embodiment 4
The hemp cloth biological treatment is as follows:
Hemp cloth after the 5g sterilization is inserted the 250mL triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of hemp cloth and embodiment 2, liquid amount is 100mL, at 35 ℃, comes unstuck respectively in the 200rpm shaking table 2 days; Usually time is then removed come unglued liquid, stops biological treatment.Hemp cloth after the biological treatment is carried out inactivation treatment, its technique is: the hemp cloth after the biological treatment and come unglued liquid mix with 1: 25 ratio, and under 90 ℃ condition, processed 1 hour, wherein the consumption of superoxide percarbonic acid ammonium is 5% of hemp cloth weight, amount of urea is 1% of hemp cloth weight, the consumption of Sodium phosphate dibasic is 2% of hemp cloth weight, and the consumption of SODIUM PHOSPHATE, MONOBASIC is 2% of hemp cloth weight.The weaving of rear preparation of coming unstuck is soft with hemp cloth, whiteness is high, prodding and itching feeling significantly reduces.
Embodiment 5
The jute roving boiling and bleaching process is as follows:
Jute thread and yarn after the 5g sterilization is inserted the 250mL triangular flask, be sub-packed at 1: 20 in the triangular flask by bath raio with the zymocyte liquid of Jute thread and yarn and embodiment 2, liquid amount is 100mL, at 40 ℃, comes unstuck respectively in the 180rpm shaking table 4 days; Usually time is then removed come unglued liquid, stops biological treatment.Jute thread and yarn after the biological treatment is carried out inactivation treatment, its technique is: the Jute thread and yarn after the biological treatment and come unglued liquid mix with 1: 8 ratio, and under 100 ℃ condition, processed 8 hours, wherein the consumption of superoxide potassium per(oxy)borate is 8% of Jute thread and yarn weight, amount of urea is 2% of Jute thread and yarn weight, the consumption of sodium-metaphosphate is 2% of Jute thread and yarn weight, the consumption of disodium ethylene diamine tetraacetate is 2% of Jute thread and yarn weight, and the consumption of tripoly phosphate sodium STPP is 2% of Jute thread and yarn weight.760 weaving Jute thread and yarn can making after coming unstuck.
Embodiment 6
The flax roving boiling and bleaching process is as follows:
Linen thread and yarn (flax roving or second hards) after the 5g sterilization is inserted the 250mL triangular flask, zymocyte liquid with linen thread and yarn and embodiment 2 is sub-packed in the triangular flask by bath raio at 1: 20, liquid amount is 100mL, at 40 ℃, comes unstuck respectively in the 220pm shaking table 1 day; Usually time is then removed come unglued liquid, stops biological treatment.Linen thread and yarn after the biological treatment is carried out inactivation treatment, its technique is: the linen thread and yarn after the biological treatment and come unglued liquid mix with 1: 15 ratio, and under 90 ℃ condition, processed 2 hours, wherein the consumption of superoxide ammonium persulphate is 2% of linen thread and yarn weight, amount of urea is 0.05% of linen thread and yarn weight, the consumption of tripoly phosphate sodium STPP is 2% of linen thread and yarn weight, and the consumption of sodium phosphate is 1% of linen thread and yarn weight.1530 weaving linen thread and yarn can making after coming unstuck.
Claims (10)
1. method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber comprises:
(1) epicoccum nigrum DB 3 strains is stored in potato dextrose agar, 30 ℃ of 220rpm cultivate 48h, add frozen damping fluid; Wherein frozen damping fluid is by potassium primary phosphate 0.0627g, dipotassium hydrogen phosphate 0.0177g, and Trisodium Citrate 0.0588g, bitter salt 0.02645g, glycerine 10mL adds water and is settled to 100mL and makes; The prescription of potato dextrose agar is: potato 200g, glucose 20g, tap water 1000mL, natural pH value;
(2) in the cooled 50mL potato glucose substratum of sterilization, bacterium one ring of access step (1) gained, shake-flask culture 48h under rotating speed 220r/min, 30 ℃ of conditions of temperature;
(3) culture medium inoculated with step (2) gained enters Flax Lignin fermention medium 5L, and shake-flask culture 48h obtains zymocyte liquid under rotating speed 220r/min, 30 ℃ of conditions of temperature; Wherein, flax fermentative medium formula is: flax raw ramie xylogen 5g, KCl 0.05g, dipotassium hydrogen phosphate 0.1g, MgSO
40.05g, FeSO
40.001g, (NH
4)
2SO
40.5g moisturizing is to the 100mL constant volume;
(4) the numb raw material after will sterilizing mixes by bath raio 1:20 with step (3) gained zymocyte liquid, at 30 ℃, processes in the 160-220rpm shaking table 1 ~ 4 day, removes fermented liquid, obtains flaxen fiber;
(5) above-mentioned flaxen fiber and come unglued liquid are mixed, under 70-100 ℃ of temperature, processed 1-8 hour, then washing, and method routinely oils, drying, namely obtaining can be for the fiber of weaving; Wherein flaxen fiber is 1:8-1:25 to the weight ratio of come unglued liquid, and the temperature of coming unstuck is 70-100 ℃, and the time is 1-8 hour;
The preserving number of described epicoccum nigrum DB 3 strains is CGMCC No.4687.
2. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1, it is characterized in that: the numb raw material described in the step (4) is flax straw, linen thread and yarn, sodolin, hemp original stem, hemp yarn, hemp cloth, the former stem of jute, Jute thread and yarn, megila, the former stem of bluish dogbane, bluish dogbane yarn or bluish dogbane fabric.
3. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1, it is characterized in that: the come unglued liquid described in the step (5) is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water; Wherein peroxide degumming agent, sequestrant and hydrogen bond disrupting agent sum are 1:8-1:25 with the weight ratio of the solution that comes unstuck.
4. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 3, it is characterized in that: the peroxide degumming agent described in the step (5) is hydrogen peroxide, Potassium Persulphate, Sodium Persulfate, ammonium persulphate, antihypo, SPC-D, percarbonic acid ammonium, potassium per(oxy)borate, Sodium peroxoborate or ammonium pertorate, and its consumption is the 0.25-8% of numb raw material weight.
5. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 3, it is characterized in that: the sequestrant described in the step (5) is sodium phosphate, tripoly phosphate sodium STPP, one or more of sodium-metaphosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, citric acid, disodium ethylene diamine tetraacetate, its consumption is the 0.1-4% of numb raw material weight.
6. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 3, it is characterized in that: the hydrogen bond disrupting agent described in the step (5) is urea, its consumption is the 0.05-2% of numb raw material weight.
7. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1 is characterized in that: the flaxen fiber described in the step (5) and the come unglued liquid ratio take weight ratio as 1:10 is mixed, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is hydrogen peroxide, and its consumption is 3% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 0.05% of numb raw material weight, and sequestrant is tripoly phosphate sodium STPP and disodium ethylene diamine tetraacetate, and its consumption is respectively 2% and 0.1% of numb raw material weight.
8. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1 is characterized in that: the flaxen fiber described in the step (5) and the come unglued liquid ratio take weight ratio as 1:25 is mixed, and the temperature of coming unstuck is 70 ℃, and the time is 3 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is the percarbonic acid ammonium, and its consumption is 5% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 1% of numb raw material weight, and sequestrant is Sodium phosphate dibasic and SODIUM PHOSPHATE, MONOBASIC, and its consumption is 2% of numb raw material weight.
9. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1 is characterized in that: the flaxen fiber described in the step (5) and the come unglued liquid ratio take weight ratio as 1:8 is mixed, and the temperature of coming unstuck is 100 ℃, and the time is 8 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is potassium per(oxy)borate, and its consumption is 8% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 2% of numb raw material weight, and sequestrant is sodium-metaphosphate, disodium ethylene diamine tetraacetate and tripoly phosphate sodium STPP, and its consumption is 2% of numb raw material weight.
10. a kind of method of utilizing epicoccum nigrum DB 3 strains to prepare flaxen fiber according to claim 1 is characterized in that: the flaxen fiber described in the step (5) and the come unglued liquid ratio take weight ratio as 1:15 is mixed, and the temperature of coming unstuck is 90 ℃, and the time is 2 hours; Wherein come unglued liquid is comprised of peroxide degumming agent, sequestrant, hydrogen bond disrupting agent and water, and the peroxide degumming agent is ammonium persulphate, and its consumption is 2% of numb raw material weight; The hydrogen bond disrupting agent is urea, and its consumption is 0.05% of numb raw material weight, and sequestrant is tripoly phosphate sodium STPP and sodium phosphate, and its consumption is respectively 2% and 1% of numb raw material weight.
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| CN106119136B (en) * | 2016-09-26 | 2019-04-26 | 中国农业科学院特产研究所 | Epicoccum nigrum and its application |
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| CN114959923A (en) * | 2022-05-05 | 2022-08-30 | 中国农业科学院麻类研究所 | Extraction auxiliary agent for jute bast fiber and preparation method and application thereof |
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| CN100441752C (en) * | 2006-09-11 | 2008-12-10 | 东华大学 | Method of preparing fiber for weave by peroxide degumming method |
| CN102010836B (en) * | 2010-08-13 | 2012-07-04 | 东华大学 | Cellulomonas fimi DA 8 bacterial strain as well as acquiring method and application thereof |
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2011
- 2011-08-30 CN CN 201110253304 patent/CN102409411B/en not_active Expired - Fee Related
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