CN103468582B - Pectinase preparation producing Aspergillus japonicus PJ01 and enzyme production method - Google Patents
Pectinase preparation producing Aspergillus japonicus PJ01 and enzyme production method Download PDFInfo
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- CN103468582B CN103468582B CN201310350534.2A CN201310350534A CN103468582B CN 103468582 B CN103468582 B CN 103468582B CN 201310350534 A CN201310350534 A CN 201310350534A CN 103468582 B CN103468582 B CN 103468582B
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Abstract
The invention relates to a bacterial strain for fermentation of orange peel powder to produce a pectinase preparation and a method of enhancing enzyme activity by a surfactant. In the invention, Aspergillus japonicus PJ01 is employed to produce the pectinase preparation, the activity of the prepared pectinase preparation is represented by pectinase activity, cellulase activity and hemicellulase activity. The production method of the pectinase preparation includes: taking crushed orange peel powder as a sole carbon source, adding the orange peel powder into an inorganic nutrient salt in a Czapek medium, then adding 0.1-0.5%(wt) of PEG4000 into a liquid Aspergillus niger fermentation medium, carrying out shaking cultivation for 3-5 days, thus significantly improving the exo-pectinase activity, the cellulase activity (in terms of CMCase enzyme) and hemicellulase activity (in terms of Xylanase) relative to the control group. This method is simple, can significantly improve the pectinase preparation production ability of Aspergillus, and has the characteristic of environmental friendliness, thereby having good application prospects.
Description
Technical field
The present invention relates to the bacterial strain of product pectase preparation and improve its enzyme enzyme producing method alive, being specifically related to a strain and utilizing orange meal fermentation produce the bacterial strain of pectase preparation and utilize tensio-active agent to improve its enzyme enzyme producing method alive.
Background technology
In Citrus procession process, can produce a large amount of fruit juice, essential oil and other by products, wherein orange peel is a kind of main solid by-product, accounts for greatly 50% of fruit fresh weight, meanwhile, the processed factory of fresh orange peel poses a big pressure to environment as waste disposal.For China, the annual skin slag that produces in the whole nation is at 5,000,000 more than t, and only citrus skin slag output is up to 650,000 t.Therefore how to utilize these " rubbish ", be developed to serial high value added product extremely urgent.
Pectin 16.0% in the chemical composition of orange peel, Mierocrystalline cellulose 22.5%, hemicellulose 6.0%, xylogen 8.6%, therefore can produce the excellent substrate of zymin, the zymin being rich in polygalacturonase, cellulase and hemicellulase of preparation as microbiological deterioration.May be used for juice extraction and clarification, coming unstuck of plant phloem tissue, extract the lemon oil in peel of Citrus reticulata Blanco, improve colourity and the stability of red wine, the fields such as animal-feed.In addition, can be used for biological enzyme and remove orange skin, biological enzyme excystation clothing is that a kind of production efficiency is high, oranges and tangerines can excystation clothing new technology that constant product quality, security are high, free from environmental pollution.
In recent years, the research of single bacterium or mixed fungus fermentation product Combizym (enzyme cocktail) receives increasing concern.Select for carbon source, the people such as Olsson, Botella find that the biomass such as mold fermentation xylogen carbon source, grape skin and corn cob can both produce the Combizyms such as cellulase, polygalacturonase and hemicellulase.Domestic patent utilizes wheat bran, stalk, beet pulp, apple residue etc. to prepare zymin for compounded carbons mostly, utilizes orange meal to prepare zymin as sole carbon source and have not been reported.
Aspergillus niger (Aspergillusniger) is commonly referred to be safe (GRAS) as production host, and can produce enzyme within the rational time, therefore becomes the very important bacterial classification of one of production zymin.But as can be seen from the document of open report, research of polygalacturonase, cellulase and hemicellulase is produced for it and mostly concentrates on above the optimization of bacterial screening, qualification and fermentation condition.But for the production of zymin, its enzyme activity level is still not high enough, therefore urgently finds the external factor limiting its enzymatic productivity and optimizes its culture condition.
As everyone knows, such as aspergillus, wood is mould waits fungi to produce extracellular enzyme when degrading plant cell walls, therefore secretion extracellular enzyme and cell permeability have direct relation, the people such as Tangnu find that the Tween-80 of suitable concn can promote wooden mould eccrine fiber element enzyme, the ability of hemicellulase and beta-glucosidase, Ceng Guang is bright waits people to significantly improve its enzyme activity by adding rhamnolipid in the cellulosic substratum of Trichoderma Viride, the people such as Callow find that rhamnolipid and Triton X-100 can promote the ability of Trichodermareesei cellulase-producing, but the degraded product enzyme of orange meal is but a blank to utilize tensio-active agent to promote.Therefore, utilize a kind of cheap, the eco-friendly tensio-active agent of hypotoxicity carries out biological fermentation orange meal production zymin and has good DEVELOPMENT PROSPECT.
Summary of the invention
One of the object of the invention is that providing a strain to utilize orange meal to ferment produces aspergillus japonicus (Aspergillus japonicus) PJ01 of pectase preparation.The aspergillus japonicus bacterial strain that the present invention relates to submits preservation on July 9th, 2013.Depositary institution is the China typical culture collection center (CCTCC) of Wuhan, China Wuhan University, and deposit number is CCTCC NO:M 2013323, and Classification And Nomenclature is aspergillus japonicus PJ01Aspergillus japonicus PJ01.
The dull and stereotyped upper 35 DEG C of cultivations of the morphological specificity of aspergillus japonicus (Aspergillus japonicus) PJ01: PDA 1 day are white hypha, and cultivate and form black Chan Bao district in 2 days, cultivating colony diameter after 4 days is 50-60mm (Fig. 4); The long 1-3mm of conidiophore, diameter 20-30 μm are transparent; Conidium ball-type, brown-black, diameter 40-60 μm; Spore diameter is about 4-6 μm, circular.
Aspergillus japonicus provided by the present invention (Aspergillus japonicus) PJ01 can be used for preparing pectase preparation, containing prozyme vigor such as polygalacturonase, cellulase, hemicellulases in zymin.Aspergillus japonicus (Aspergillus japonicus) PJ01 can grow and produce enzyme, zymotechnique simple possible on the liquid nutrient medium that orange meal is sole carbon source, and it is rapid that enzyme is produced in growth, and orange peel waste utilization is abundant, added value of product high.
Two of the object of the invention is that providing a kind of above-mentioned aspergillus japonicus (Aspergillus japonicus) PJ01 to ferment improves produced prozyme enzyme method alive.
1) slant culture: by aspergillus japonicus (Aspergillus japonicus) PJ01 spore inoculating on PDA slant medium, cultivates 3-5 days, preservation to 4 DEG C after spore maturation for 34-36 DEG C;
2) collecting cells: wash lower PDA slant medium aspergillus spore with the physiological saline of the tween-80 containing mass percent 0.02-0.1%, make spore suspension;
3) fermentation culture: peel of Citrus reticulata Blanco 50-60 DEG C of oven dry, pulverized 10-20 mesh sieve; Preparation Cha Shi medium salts solution, 250mL triangular flask liquid amount 50-100mL, adds the orange meal of mass percent 1-3%, the Surfactant PEG 4000 of mass percent 0.1-0.5%; 121 DEG C of autoclaving 15-25min, inoculating spores suspension to 1 × 10
5-1 × 10
6individual spore/mL, cultivates 48-72h in constant-temperature table, temperature 34-36 DEG C, natural pH, rotating speed 150-180r/min.
The Cha Shi medium salts solution formula (g/L) that the present invention adopts: NaNO
33-5, K
2hPO
31, KCl 0.5, MgSO
47H
2o 0.5, FeSO
47H
2o 0.01.
The selection of tensio-active agent of the present invention: 250mL triangular flask liquid amount 50-100mL, adds different surfaces promoting agent Tween-20, Tween-80, PEG4000, PEG6000, Triton X-100 and washing powder 0.3% (wt).121 DEG C of autoclaving 15-25min, inoculation aspergillus spore suspension concentration to 1 × 10
5-1 × 10
6individual/mL, cultivates 48-72h in constant-temperature table, temperature 34-36 DEG C, natural pH, rotating speed 150-180r/min.Because in orange meal, pectin composition content proportion is maximum, therefore selected pectinase activity comparatively contrasts and increases maximum PEG4000 further experiment (Fig. 1).
The optimal concentration of Surfactant PEG 4000 of the present invention is selected: the PEG4000 adding 0.1-0.9% (wt) concentration, utilizes DNS method to measure pectinase activity (Fig. 2), cellulase activity and hemicellulose enzyme activity (Fig. 3).
Enzyme liquid extracting method of the present invention: the centrifugal 10-15min of product 8000r/min fermentation obtained removes solid substance, and supernatant liquor is enzyme liquid, adopts DNS method to measure pectinase activity, cellulase activity and hemicellulose enzyme activity.
Enzyme activity analytical procedure:
A, pectinase activity measure: the enzyme liquid 0.5mL getting the dilution of suitable damping fluid adds the pectin solution of 1.5mL 0.5% (W/V), and (this solution is by pH=5.0, concentration 0.1M acetate buffer configures) in 25mL tool plug test tube, 2mL DNS termination reaction is added after 45 DEG C of reaction 10min, boiling water bath 5min, flowing water cools, keep the skin wet to scale with distilled water, 540nm place measures light absorption value.Blank is for before adding enzyme liquid, and first add the DNS termination reaction of 2mL, other steps are identical.Enzyme is lived and is defined: 1mL crude enzyme liquid is a Ge Meihuo unit (U) at the raw 1 μm of ol D-galacturonic acid of 1min bottom exploded produce.
B, cellulase activity measure: the enzyme liquid 0.5mL getting the dilution of suitable damping fluid adds the CMC-Na solution of 1.5mL 1.0% (W/V), and (this solution is by pH=4.8, concentration 0.05M citrate buffer solution configures) in 25mL tool plug test tube, 2mL DNS termination reaction is added after 50 DEG C of reaction 30min, boiling water bath 5min, flowing water cools, keep the skin wet to scale with distilled water, 540nm place measures light absorption value.Blank is for before adding enzyme liquid, and first add the DNS termination reaction of 2mL, other steps are identical.Enzyme is lived and is defined: 1mL crude enzyme liquid is a Ge Meihuo unit (U) at the raw 1 μm of ol glucose of 1min bottom exploded produce.
C, hemicellulose enzyme activity determination: the enzyme liquid 1mL getting the dilution of suitable damping fluid adds the xylan solution of 1mL 1.0% (W/V), and (this solution is by pH=5.0, concentration 0.1M acetate buffer configures) in 15mL tool plug test tube, 2.5mL DNS termination reaction is added after 50 DEG C of reaction 10min, boiling water bath 5min, flowing water cools, keep the skin wet to scale with distilled water, 540nm place measures light absorption value.Blank is for before adding enzyme liquid, and first add the DNS termination reaction of 2.5mL, other steps are identical.Enzyme is lived and is defined: 1mL crude enzyme liquid is a Ge Meihuo unit (U) at the raw 1 μm of ol wood sugar of 1min bottom exploded produce.
The present invention is on the basis of optimization of fermentation conditions, and further comparative studies tensio-active agent Tween-20, Tween-80, PEG4000, PEG6000, Triton X-100 and washing powder produce the impact of enzyme activity to bacterium.Found that the improvement that PEG4000 lives to three of bacterial strain kinds of enzyme enzymes compared with other type table surface-active agents is comparatively outstanding.Add the PEG4000 of 0.1%-0.5% (wt), cultivate through 3-5 days shaking tables, the circumscribed pectolase vigor (Exo-Pectinase) of bacterial strain improves 25.48%, cellulase activity (in CMCase enzyme) improves 16.39%, and hemicellulose enzyme activity (in zytase Xylanase) improves 11.66%.Because PEG has good oilness, thermostability, the good characteristics such as hypotoxicity and difficult volatility, is therefore applicable to industrial enzyme preparation additive.The inventive method is simple, can significantly improve the ability that aspergillus produces pectase preparation, and have eco-friendly feature, therefore have good application prospect.
Accompanying drawing explanation
Fig. 1 is different surfaces active species (Tween-20, Tween-80, PEG4000, PEG6000, TritonX-100 and the washing powder) impact on circumscribed pectolase in pectase preparation;
Fig. 2 is the impact that different concns Surfactant PEG 4000 is lived on circumscribed pectolase in pectase preparation;
Fig. 3 is the impact that different concns Surfactant PEG 4000 is lived on pectase preparation cellulase and hemicellulase;
Fig. 4 is bacterial strain PDA slat chain conveyor of the present invention 4 days bacterium colony figure.
Embodiment
In order to understand the present invention further, be described the preferred embodiments of the invention below in conjunction with embodiment, these describe just as further illustrating the features and advantages of the present invention instead of limiting to the claimed invention.
Embodiment 1: medium preparing
Prepared by orange meal: peel of Citrus reticulata Blanco 50-60 DEG C of oven dry, pulverized 10-20 mesh sieve, placing glass moisture eliminator is for subsequent use.
Slant medium (g/L): peeled potatoes 200, glucose 20, agar 20, pH nature, water 1000mL; 121 DEG C of sterilizing 15min, holding test tubes inclined-plane; Inoculation after cooling, cultivates 4 days for 35 DEG C.
Fermention medium (g/L): orange meal 20, NaNO
35, K
2hPO
31, KCl 0.5, MgSO
47H
2o 0.5, FeSO
47H
2o 0.01.
Embodiment 2: prepared by spore suspension
Wash lower aspergillus japonicus spore with the physiological saline containing 0.1wt% tween-80, make spore suspension, count with blood counting chamber and be placed to 4 DEG C of Refrigerator stores.
Embodiment 3: the screening of aspergillus japonicus (Aspergillus japonicus) PJ01 and authentication method
A, primary dcreening operation: the branch that rots from Yue Lu mountain soil sample, tangerine garden mould sample, kiwi fruit tree garden soil sample, Yue Lu mountain, fruit market rot 15 collected specimens such as orange.Sample thief 5g is added in the 250mL triangular flask containing 100mL fermention medium, in 35 DEG C, enrichment culture, after 3 days, is got 1mL nutrient solution and is diluted to spore count 10 under 170r/min shaking table condition
-6-10
-8individual/mL, getting that 0.2mL diluent is uniformly coated on is on the screening plate culture medium of carbon source with pectin, Xylo-Mucine (CMC-Na) and xylan respectively, cultivate after 3 days, the larger fungal strain of transparent circle totally 20 is filtered out by the method for congo red staining, carry out further drawing dull and stereotyped purifying bacterial strain, be finally forwarded on PDA slant medium and cultivate preservation.
B, multiple sieve: peel of Citrus reticulata Blanco 60 DEG C oven dry, pulverized 10-20 mesh sieve; Preparation Cha Shi medium salts solution, 250mL triangular flask liquid amount 100mL, adds the orange meal of mass percent 2%, 121 DEG C of autoclaving 15min, inoculation primary dcreening operation spore suspension to 1 × 10
6individual spore/mL, cultivates 72h in constant-temperature table, temperature 35 DEG C, natural pH, rotating speed 170r/min.For detecting circumscribed pectolase, CMCase enzyme and Xylanase activity after fermented liquid is centrifugal.Higher bacterial strain aspergillus japonicus (Aspergillus japonicus) PJ01 of circumscribed pectolase, CMCase enzyme and Xylanase activity is obtained by screening.
C, identification of strains: to be increased identify by ITS sequence, showing that this sequence and Aspergillusjaponicus belong to similarity is 100%, proves the new strains of aspergillus japonicus, final called after aspergillus japonicus PJ01Aspergillus japonicus PJ01.
Embodiment 4: produce enzyme and cultivate
250mL triangular flask adds 75mL fermention medium, adds PEG4000 0.1% (wt).121 DEG C of autoclaving 15min, inoculation aspergillus japonicus spore suspension concentration is 1 × 10
6individual/mL, cultivates 72h in constant-temperature table, temperature 35 DEG C, natural pH, rotating speed 170r/min.Circumscribed pectolase vigor 68.72U/mL, cellulase activity (in CMCase enzyme) 1.33U/mL, hemicellulase (in zytase) vigor 12.00U/mL, comparatively contrast raising 12.95%, 9.02% and 5.17% respectively.
Embodiment 5: produce enzyme and cultivate
250mL triangular flask adds 75mL fermention medium, adds PEG4000 0.3% (wt).121 DEG C of autoclaving 15min, inoculation aspergillus japonicus spore suspension concentration is 1 × 10
6/ mL, cultivates 72h in constant-temperature table, temperature 35 DEG C, natural pH, rotating speed 170r/min.Circumscribed pectolase vigor 76.34U/mL, cellulase activity (in CMCase enzyme) 1.42U/mL, hemicellulase (in zytase) vigor 12.74U/mL, comparatively contrast raising 25.48%, 16.39% and 11.66% respectively.
Embodiment 6: produce enzyme and cultivate
250mL triangular flask adds 75mL fermention medium, adds PEG4000 0.5% (wt).121 DEG C of autoclaving 15min, inoculation aspergillus japonicus spore suspension concentration is 1 × 10
6/ mL, cultivates 72h in constant-temperature table, temperature 35 DEG C, natural pH, rotating speed 170r/min.Circumscribed pectolase vigor 70.12U/mL, comparatively contrasts raising 15.25%; Cellulase activity (in CMCase enzyme) 1.40U/mL, comparatively contrasts raising 14.75%; Hemicellulose enzyme activity (in zytase) 10.9U/mL, comparatively contrasts reduction by 4.47%.
Claims (2)
1. aspergillus japonicus (Aspergillusjaponicus) PJ01 of pectase preparation is produced in a strain, and its deposit number is CCTCC NO:M2013323.
2. adopt aspergillus japonicus strain fermentation according to claim 1 to prepare a method for pectase preparation, it is characterized in that:
1) slant culture: by aspergillus japonicus (Aspergillusjaponicus) PJ01 spore inoculating on PDA slant medium, cultivates 3-5 days, preservation to 4 DEG C after spore maturation for 34-36 DEG C;
2) collecting cells: wash lower PDA slant medium aspergillus spore with the physiological saline of the tween-80 containing mass percent 0.02-0.1%, make spore suspension;
3) fermentation culture: peel of Citrus reticulata Blanco 50-60 DEG C of oven dry, pulverized 10-20 mesh sieve; Preparation Cha Shi medium salts solution, 250mL triangular flask liquid amount 50-100mL, adds the orange meal of mass percent 1-3%, the Surfactant PEG 4000 of mass percent 0.1-0.5%; 121 DEG C of autoclaving 15-25min, inoculating spores suspension to 1 × 10
5-1 × 10
6individual spore/mL, cultivates 48-72h in constant-temperature table, temperature 34-36 DEG C, natural pH, rotating speed 150-180r/min.
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| CN104388361B (en) * | 2014-12-03 | 2017-03-08 | 厦门大学 | The marine bacteria of one plant of product pectase and its application |
| CN104818223A (en) * | 2015-05-18 | 2015-08-05 | 湖北工业大学 | Zygoascus sp. and application thereof |
| CN105462866B (en) * | 2015-12-24 | 2018-11-20 | 邵阳学院 | The activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application |
| CN110153440B (en) * | 2019-05-14 | 2022-03-22 | 桂林理工大学 | Method for green preparation of nano-silver from aspergillus japonicus fermentation liquor and application |
| CN112877381A (en) * | 2021-01-08 | 2021-06-01 | 桂林理工大学 | Preparation method and application for degrading passion fruit peel polysaccharide by utilizing enzyme produced by aspergillus japonicus PJ01 |
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| CN101665769A (en) * | 2009-04-03 | 2010-03-10 | 中南大学 | Bacterial strain with high protopectinase yield and method for extracting pectin by fermentation |
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Non-Patent Citations (4)
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| 微生物果胶酶及其在食品方面的应用;崔福绵;《微生物学通报》;19850427(第02期);第92页右栏倒数第三段 * |
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