CN105462866B - The activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application - Google Patents
The activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application Download PDFInfo
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Abstract
The invention discloses a kind of activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application, yeast strain is yeast strain SYU-F201-04, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.10760.The activation culture original washing powder of yeast strain includes in parts by weight：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, beautiful 7.7~8.6 parts of cone capsule algae, 3.3 parts of the first auxiliary agent, the first auxiliary agent include that mass ratio is 33：16 carboxymethyl chitosan and microcrystalline cellulose.Above-mentioned activation culture original washing powder, the activation medium being prepared can recovery activated yeast strain.After the yeast strain of activation increases bacterium using excystation clothing culture medium, excystation clothing effect is good, can effectively meet the needs of de- navel orange peel.
Description
Technical field
The present invention relates to navel orange excystation collar domains, particularly, are related to a kind of activation culture original washing powder of yeast strain.In addition,
The invention further relates to the activation method of above-mentioned yeast strain, the excystation clothing medium powder of above-mentioned yeast strain and above-mentioned yeast strains
Application.
Background technique
Navel orange is the citrus breeding of Rutaceae Citrus, and extensively, Hunan is located at national Citrus Industry for plantation in south China
Band middle section center, navel orange is resourceful, and processing industry is larger.The beverage of the pulp containing navel orange be formed nearly ten years it is new
Emerging consumption hot spot, wherein the navel orange pulp to suspend is navel orange cyst (also referred to as orange juice born of the same parents), by fruit outside navel orange in navel orange fruit ball
Skin and peel package.To obtain navel orange cyst, mechanical peeling method is taken in industrialization processing, efficiency is higher, this this group of field
Team has obtained invention patent mandate (a kind of automatic fruits and vegetables skiving machine ZL201310020528.0).
Though belong to Citrus with tangerine, after removing exocarp, the peel of navel orange connect with pulp it is very close, no matter
Mechanical Method and manual method all easy damaged pulp then cause environmental pollution according to acid-base method, with development resource economizing type, environment
Friendly industry is inconsistent.So needing to research and develop efficient, safe and environment-friendly excystation clothing method.
Part microorganism can produce a variety of enzymes and decomposed plant component due to living necessities, be converted to the battalion that Institute of Micro-biology needs
Support substance.Targetedly screening is suitble to the microorganism of de- navel orange peel, produces navel orange cyst with fermentation method, can reduce cost,
It improves efficiency, takes into account safety and environmental protection.
Summary of the invention
The present invention provides a kind of activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application, with
Solve navel orange peel de- the technical issues of taking method.
The technical solution adopted by the present invention is as follows：
One aspect of the present invention provides a kind of activation culture original washing powder of yeast strain, and yeast strain is yeast strain SYU-
F201-04, depositary institution are China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
NO.10760。
The activation culture original washing powder of yeast strain includes in parts by weight：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, beautiful cone
7.7~8.6 parts of capsule algae, 3.3 parts of the first auxiliary agent, the first auxiliary agent include that mass ratio is 33：16 carboxymethyl chitosan and crystallite is fine
Dimension element.
Another aspect of the present invention provides a kind of activation method of yeast strain, includes the following steps：
It handles, is sieved through mechanochemistry after above-mentioned activation culture original washing powder is mixed, after being dissolved in water, sterilizing obtains activation training
Support base.
Yeast strain is seeded in activation medium, is cultivated at 37 DEG C for 24 hours, the yeast strain activated.
Further, the operation of progress mechanochemistry processing is specially after the mixing of activation culture original washing powder：
Activation culture original washing powder is mixed and is handled using vibromill, 19~21 DEG C of cooling cycle water temperature, frequency 16Hz,
17~18min of time is handled under 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 7.5g
12~13min.
Another aspect of the present invention provides a kind of application of the yeast strain of activation in de- navel orange peel, including following step
Suddenly：
After above-mentioned excystation clothing medium powder is mixed, motorized processing, sieving, after being dissolved in water, sterilizing is taken off
Peel culture medium.
The yeast strain of activation is inoculated into excystation clothing culture medium and is cultivated for 24 hours, excystation clothing working solution is obtained.
Excystation clothing working solution is warming up to 40 DEG C, the navel orange of peeling is added, under conditions of 4.5,45~50 DEG C of pH value, is stirred
90~120min of constant temperature is mixed and kept, navel orange peel is sloughed.
Further, the operation of progress mechanochemistry processing is specially after the mixing of excystation clothing medium powder：
Excystation clothing medium powder is mixed and is handled using vibromill, in 19~21 DEG C of cooling cycle water temperature, frequency
Time 14min is handled under 16Hz, 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 4.4g
7min。
The invention has the advantages that：Above-mentioned activation culture original washing powder, the activation medium being prepared can recover
Activated yeast strain.After the yeast strain of activation increases bacterium using excystation clothing culture medium, excystation clothing effect is good, can effectively meet de- navel
The demand of orange peel.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
Below with reference to figure, the present invention is described in further detail.
Detailed description of the invention
The attached drawing constituted part of this application is used to provide further understanding of the present invention, schematic reality of the invention
It applies example and its explanation is used to explain the present invention, do not constitute improper limitations of the present invention.In the accompanying drawings：
Fig. 1 is the activation method flow chart of the yeast strain of the preferred embodiment of the present invention；
Fig. 2 is applicating flow chart of the yeast strain of the activation of the preferred embodiment of the present invention in de- navel orange peel.
Specific embodiment
The embodiment of the present invention is described in detail below in conjunction with attached drawing, but the present invention can be defined by the claims
Implement with the multitude of different ways of covering.
Referring to Fig.1, one aspect of the present invention provides a kind of activation culture original washing powder of yeast strain, the activation training of yeast strain
Feeding original washing powder includes in parts by weight：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, beautiful 7.7~8.6 parts of cone capsule algae, the first auxiliary agent
3.3 parts, the first auxiliary agent includes that mass ratio is 33：16 carboxymethyl chitosan and microcrystalline cellulose.Wherein orange peel powder is navel orange fruit
Skin crushes gained powder after drying or drying, tangerine seed powder is that gained powder is crushed after citrus fruit seed is dried or dried.
Yeast strain SYU-F201-04 is isolated from navel orange leaf tissue, and it is micro- to be deposited in China on April 28th, 2015
Biological inoculum preservation administration committee common micro-organisms center, depositary institution address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number, deposit number is CGMCC NO.10760.The present invention uses SYU-F201-04 bacterium, and classification naming is Meyerozyma sp..It should
Bacterium function is special, and excystation clothing effect is good, but also more special to culture medium demand, no matter dry powder or glycerol save, is in suspend mode
It must be recovered and be activated using activation medium after state, otherwise performance is unable to satisfy excystation clothing demand.
In parts by weight, in activation culture original washing powder：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, beautiful cone capsule algae 7.7~
8.6 parts, 3.3 parts of the first auxiliary agent.Orange peel powder, tangerine seed powder, the beautiful water content for boring capsule algae are 3~5%.
In activation culture based formulas, orange peel powder and tangerine seed powder ratio are 21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, the ratio
Example is obtained through test of many times, and pectin is more if orange peel powder ratio height, is unfavorable for thallus activation, recovery；If tangerine seed powder ratio is high
Then fat content is high, and microbial metabolism is slow, reduces active rate.This condition is the key that one of control recovery.
Different from Zhui Nang algae other algaes of section, beautiful cone capsule algae makees for SYU-F201-04 bacterium with apparent growth promotion
With.It is embodied in beautiful cone capsule algae additive amount at 7.7~8.6 parts, the strain recovery time can be shortened 140% or more,
But because of pectin in the algae containing specific proportions and siliceous, when the few activity of revival phase bacterium is low, when additive amount is more than 8.6 parts, work
Change effect to substantially reduce instead.And it is lower than 7.7 parts, the thin cone of beautiful cone capsule algae, middle part slightly expand the subtle wave edges of tool
Structure effect show again it is very unobvious, it is difficult to guarantee that target thallus makes full use of.So beautiful cone capsule algae additive amount exists
7.7~8.6 parts, this condition is one of another key of control recovery.
The invention has the advantages that：Above-mentioned activation culture original washing powder, the activation medium being prepared can recover
Activated yeast strain.After the yeast strain of activation increases bacterium using excystation clothing culture medium, excystation clothing effect is good, can effectively meet de- navel
The demand of orange peel.
One aspect of the present invention provides a kind of activation method of yeast strain, referring to Fig.1, includes the following steps：
S110：It handles, is sieved through mechanochemistry after activation culture original washing powder is mixed, after being dissolved in water, sterilizing obtains activation training
Support base.
S120：Yeast strain is seeded in activation medium, is cultivated at 37 DEG C for 24 hours, the yeast strain activated.
Orange peel powder, tangerine seed powder, beautiful cone capsule algae can be first crushed to the coarse powder of 60~80 mesh respectively, can also directly buy this
The raw material of particle size, or suitably prolonged using the orange peel powder, tangerine seed powder, beautiful cone capsule algae of greater particle size in mechanochemistry processing
The long processing time.Orange peel powder, tangerine seed powder and the motorized processing of beautiful cone capsule algae, i.e. orange peel powder, tangerine seed powder, beautiful cone capsule algae
Between by crush, squeeze, hit and shearing means when, chemically react.
Activation culture original washing powder carries out mechanochemistry processing after mixing, and can carry out in vibromill, activation culture original washing powder is shaking
In dynamic mill, the effect of crushing, shock and shearing occurs simultaneously for activation culture original washing powder, and solid-state machinery occurs for each activation culture original washing powder
Learn reaction.Obvious activation culture original washing powder can also carry out the step in other devices, as long as can make to send out between each activation culture original washing powder
The effect that fecula is broken, hits and shears.400 meshes can be crossed when sieving.Powder after sieving can be 121 after being dissolved in water
DEG C, 20min sterilizes to obtain the activation medium suitable for SYU-F201-04.
Yeast strain is cultivated for 24 hours in activation medium at 37 DEG C.Every 100g is dissolved in 900ml deionized water, and 121 DEG C,
20min sterilizes to obtain SYU-F201-04 activation medium.105 SYU-F201- will be accessed in above-mentioned 1000ml activation medium
After 04 bacterium, 37 DEG C, shaking flask culture for 24 hours, obtains activation culture liquid, and yeast strain activation is completed.
Optionally, the operation through crushing, shock and shear treatment is specially after the mixing of activation culture original washing powder：
Activation culture original washing powder is mixed and is handled using vibromill, 19~21 DEG C of cooling cycle water temperature, frequency 16Hz,
17~18min of time is handled under 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 7.5g
12~13min.Under this condition, activation culture original washing powder undergoes crushing, shock and shear action, each raw material simultaneously in vibromill
Solid-state machinery chemical reactions different twice, high treating effect occurs.Vibromill can grind for high energy vibration.At through vibromill
Sieving obtains activation original washing powder after reason.
Another aspect of the present invention provides a kind of excystation clothing medium powder of yeast strain, which is characterized in that with parts by weight
Meter includes：77.2 parts of orange peel powder, 32.5~33.9 parts of peel powder, 4.8 parts of light red goldc grains algae, 6.0 parts of the second auxiliary agent.Second auxiliary agent
It is 12 including mass ratio:5 saleratus saleratus and hydroxypropyl-β-cyclodextrin.
After yeast strain SYU-F201-04 activation, it is necessary to carry out increasing bacterium using excystation clothing culture medium, otherwise performance can not
Meet excystation clothing demand.
Orange peel powder, peel powder, light red goldc grains algae water content can be 3~5%.In excystation clothing culture medium prescription, orange peel
Powder and peel powder ratio are 77.2 parts of orange peel powder, and 32.5~33.9 parts of peel powder, which obtained through test of many times.If orange peel
The excessively high then pigment of powder ratio is more, is unfavorable for later product and stablizes, and increases water consume；It will lead to excystation if peel powder ratio is higher
The too early consumption of clothing ingredient, and formed working solution mobility reduces, processing capacity is low.
Applicant proves that light red goldc grains algae has apparent growth promoting function for SYU-F201-04 bacterium through test of many times.
It is embodied in light red goldc grains algae additive amount at 4.8 parts, the strain doubling time can be shortened 32% or more, but because in the algae
Chromatoplast containing specific proportions is increasing the bacterium stage, and when additive amount is more than 4.9 parts, activation effect substantially reduces instead.And it is lower than
4.7 parts, the structure effect of the spherical pale red transparent tool aperture of the 8-12 μm of softgel shell of light red goldc grains algae shows very unobvious again, it is difficult to
Guarantee that target thallus makes full use of.So light red goldc grains algae additive amount 4.8 parts of this conditions be the key that control increase bacterium it
One.
Another aspect of the present invention provides a kind of application of the yeast strain of activation in de- navel orange peel, including following step
Suddenly：
S210：After excystation clothing medium powder is mixed, motorized processing, sieving, after being dissolved in water, sterilizing obtains excystation
Clothing culture medium.
S220：The yeast strain of activation is inoculated into excystation clothing culture medium and is cultivated for 24 hours, excystation clothing working solution is obtained.
S230：Excystation clothing working solution is warming up to 40 DEG C, the navel orange of peeling is added, in 4.5,45~50 DEG C of condition of pH value
Under, 90~120min of constant temperature is stirred and maintained, navel orange peel is sloughed.
Orange peel powder, peel powder, light red goldc grains algae are crushed to the coarse powder of 60~80 mesh respectively, can also directly buy the partial size
The raw material of size, or use the orange peel powder, peel powder, light red goldc grains algae of greater particle size in mechanochemistry processing, it is appropriately extended
Handle the time.Orange peel powder, peel powder, light red goldc grains algae water content 3~5%.
Excystation clothing culture medium raw material mixes laggard crushing of passing through, shock and shear treatment, can carry out in vibromill.Excystation
In vibromill, the effect for crushing, hitting and shearing, each excystation clothing occur clothing culture medium raw material simultaneously for excystation clothing culture medium raw material
Solid-state machinery chemical reaction occurs for culture medium raw material.Obvious excystation clothing culture medium raw material can also carry out the step in other devices
Suddenly, as long as the effect of the impact force and shearing force of bootable mechanico-chemical reaction occurs between each excystation clothing culture medium raw material can be made i.e.
It can.400 meshes can be crossed when sieving.Excystation clothing medium powder can be at 121 DEG C after being dissolved in water, and 20min sterilizes to obtain SYU-F201-04
Excystation clothing culture medium.
The yeast strain of activation is inoculated into excystation clothing culture medium and is cultivated for 24 hours, increasing bacterium is carried out, obtains the work of excystation clothing
Liquid.Navel orange is peeled, can peel by hand or is peeled using skiving machine.Excystation clothing working solution is stirred evenly, jacketed pan can be passed through
Gas bath is warming up to 40 DEG C.The navel orange of peeling is added, lemon tune pH value can be used to 4.5, under conditions of 45~50 DEG C, constantly
90~120min of constant temperature is stirred and maintained, navel orange peel is sloughed, obtains excystation clothing navel orange fruit ball.After fruit ball warp crosses dispersion machine processing
Orange juice born of the same parents are obtained, the beverage of the pulp containing navel orange is used to prepare.
Optionally, the operation of progress mechanochemistry processing is specially after the mixing of excystation clothing medium powder：
Excystation clothing culture medium raw material is mixed and is handled using vibromill, in 19~21 DEG C of cooling cycle water temperature, frequency
Time 14min is handled under 16Hz, 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 4.4g
7min。
Under this condition, excystation clothing culture medium raw material undergoes crushing, shock and shear action, each original simultaneously in vibromill
Solid-state machinery chemical reactions different twice, high treating effect occur for material.Vibromill can grind for high energy vibration.Through vibromill
Sieving obtains excystation clothing medium powder after processing.
Optionally, excystation clothing working solution is warming up to 40 DEG C, the navel orange of peeling is added, in 4.5,45~50 DEG C of item of pH value
Under part, 90~120min of constant temperature is stirred and maintained, navel orange peel is sloughed.Under this condition, saccharomycete takes off the efficiency of navel orange peel more
It is high.
Embodiment 1
The present embodiment investigates SYU-F201-04 bacterium CGMCC NO.10760 fermentation liquid of the invention in liquid fermentation liquid to navel
The removal effect of orange peel.A kind of excystation clothing liquid of SYU-F201-04 bacterium of the invention is prepared using following methods：
Activation medium preparation process is：By orange peel powder, tangerine seed powder, that beautiful cone capsule algae is crushed to 60~80 purposes respectively is thick
Powder, by parts by weight of raw materials ingredient：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, 8.0 parts of cone capsule algae beautiful, the first 3.3 parts of auxiliary agent；
First auxiliary agent is by mass ratio 33：16 carboxymethyl chitosan and microcrystalline cellulose is constituted.Orange peel powder, tangerine seed powder, beautiful cone capsule algae
Water content 3~5%.Three kinds of coarse powder are mixed in the ratio and are mixed again with the first auxiliary agent.High energy vibration is used after mixing
Mill is handled, and at 20 DEG C of cooling cycle water temperature, frequency 16Hz, 8~9g of rod iron acceleration, handles time 17.5min, then
2.5min is shut down, then with rod iron acceleration 7.5g, handles 12~13min of time, solid-state machineries different twice occurs for each raw material
Chemical reaction.400 meshes are crossed, activation medium powder is obtained.Every 100g activation medium powder is dissolved in 900ml deionized water,
121 DEG C, 20min sterilizes to obtain SYU-F201-04 activation medium.
After accessing 105 SYU-F201-04 bacterium in above-mentioned 1000ml activation medium, 37 DEG C, shaking flask culture for 24 hours, is obtained
Activation culture liquid.It can use liquid 20ml access 1000ml excystation clothing culture medium, shaking flask culture for 24 hours, obtains excystation clothing working solution, can use
To take off the peel of 10kg navel orange.
Excystation clothing culture medium preparation process is：Orange peel powder, peel powder, light red goldc grains algae are crushed to 60~80 purposes respectively
Coarse powder, by parts by weight of raw materials ingredient：77.2 parts of orange peel powder, 33 parts of peel powder, 4.8 parts of light red goldc grains algae, the second 6.0 parts of auxiliary agent；
Second auxiliary agent is by mass ratio 12：5 hydroxypropyl-β-cyclodextrin is constituted.Orange peel powder, peel powder, light red goldc grains algae water content 3
~5%.Three kinds of coarse powder are mixed in the ratio and are mixed again with the second auxiliary agent.It is handled after mixing using high energy vibration mill,
At 20 DEG C of cooling cycle water temperature, frequency 16Hz, 8~9g of rod iron acceleration, time 14min is handled, 2.5min is then shut down, then
With rod iron acceleration 4.4g, time 7min is handled, it is anti-that solid-state machinery chemistry different twice occurs for each excystation clothing culture medium raw material
It answers, crosses 400 meshes, obtain excystation clothing medium powder, every 150g is dissolved in 850ml deionized water, and 121 DEG C, 20min sterilizes to obtain
SYU-F201-04 excystation clothing culture medium.
Excystation clothing operates：Navel orange 50kg after by skiving machine punching two ends and peeling, obtains navel of the about 40kg containing peel
Orange fruit ball, it is spare.Above-mentioned excystation clothing working solution is stirred evenly, the bath of jacketed pan vapour is warming up to 40 DEG C, and the navel peeled then is added
Orange (navel orange:Mass ratio=1 of working solution:3) it, is constantly stirred under the conditions of 45~50 DEG C of temperature with citric acid tune pH value to 4.5
90~120min of constant temperature is mixed and kept, excystation clothing navel orange fruit ball is obtained, fruit ball warp obtains orange juice born of the same parents after crossing dispersion machine processing.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of activation culture original washing powder of yeast strain, which is characterized in that yeast strain is yeast strain SYU-F201-04, is protected
Hiding unit is China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC NO.10760；
The activation culture original washing powder of the yeast strain includes in parts by weight：21.7 parts of orange peel powder, 18.8 parts of tangerine seed powder, beautiful cone
7.7~8.6 parts of capsule algae, 3.3 parts of the first auxiliary agent, first auxiliary agent include that mass ratio is 33：16 carboxymethyl chitosan and micro-
Crystalline cellulose.
2. a kind of activation method of yeast strain, which is characterized in that include the following steps：
It handles, is sieved through mechanochemistry after activation culture original washing powder in claim 1 is mixed, after being dissolved in water, sterilizing is lived
Change culture medium；
Yeast strain is seeded in the activation medium, is cultivated at 37 DEG C for 24 hours, the yeast strain activated；The ferment
Mother strains are yeast strain SYU-F201-04, and depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms
Center, deposit number are CGMCC NO.10760.
3. the activation method of yeast strain according to claim 2, which is characterized in that after the activation culture original washing powder mixes
Carry out mechanochemistry processing operation be specially：
The activation culture original washing powder is mixed and is handled using vibromill, 19~21 DEG C of cooling cycle water temperature, frequency 16Hz,
17~18min of time is handled under 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 7.5g
12~13min.
4. a kind of excystation clothing medium powder of yeast strain, which is characterized in that the yeast strain is yeast strain SYU-F201-
04, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
NO.10760；
The excystation clothing medium powder includes in parts by weight：77.2 parts of orange peel powder, 32.5~33.9 parts of peel powder, light red goldc grains
4.8 parts of algae, 6.0 parts of the second auxiliary agent；Second auxiliary agent includes that mass ratio is 12:5 saleratus saleratus and hydroxy propyl-Beta-ring
Dextrin.
5. application of the yeast strain for the activation that the activation method in a kind of Claims 2 or 3 obtains in de- navel orange peel,
It is characterized in that, includes the following steps：
After excystation clothing medium powder in claim 4 is mixed, motorized processing, sieving, after being dissolved in water, sterilizing is obtained
Excystation clothing culture medium；
The yeast strain of the activation is inoculated into the excystation clothing culture medium and is cultivated for 24 hours, excystation clothing working solution is obtained；
The excystation clothing working solution is warming up to 40 DEG C, the navel orange of peeling is added, under conditions of 4.5,45~50 DEG C of pH value, is stirred
90~120min of constant temperature is mixed and kept, navel orange peel is sloughed.
6. application of the yeast strain of activation according to claim 5 in de- navel orange peel, which is characterized in that described de-
Peel medium powder mix after carry out mechanochemistry processing operation be specially：
The excystation clothing medium powder is mixed and is handled using vibromill, in 19~21 DEG C of cooling cycle water temperature, frequency
Time 14min is handled under 16Hz, 8~9g of rod iron acceleration, then shuts down 2.5min, then the time is handled with rod iron acceleration 4.4g
7min。
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