CN107326453B - One kind cleaning degumming method of bast fiber - Google Patents
One kind cleaning degumming method of bast fiber Download PDFInfo
- Publication number
- CN107326453B CN107326453B CN201710533841.2A CN201710533841A CN107326453B CN 107326453 B CN107326453 B CN 107326453B CN 201710533841 A CN201710533841 A CN 201710533841A CN 107326453 B CN107326453 B CN 107326453B
- Authority
- CN
- China
- Prior art keywords
- liquid
- degumming
- sodium
- boiling
- cooking liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Textile Engineering (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
The present invention relates to one kind to clean degumming method of bast fiber, belongs to weaving, biology and field of environment protection.One kind cleaning degumming method of bast fiber, including degumming step, cooking liquor used is the dilution of cellulose extractant, the raffinate of Liang containing Gu≤10%, except at least one of residue and waste water after lignin in the degumming step, the extractant is mixed gained by A liquid with B liquid, the A liquid is lye, and the B liquid is made of crude enzyme liquid and enzyme stabilizers, wherein, the crude enzyme liquid is the clear crude enzyme liquid obtained after separating thallus after Bacillus subtillis is fermented.The present invention mainly uses crude enzyme liquid, sig water to handle flaxen fiber in a bath, so that the colloid in raw ramie varies widely on molecular structure, the stability of colloidal complex is seriously destroyed, alternating and circulation that swelling-modification-removes colloid are formed, achievees the purpose that quickly to carry out degumming to flaxen fiber under normal temperature condition.
Description
Technical field
The present invention relates to one kind to clean degumming method of bast fiber, belongs to weaving, biology and field of environment protection.
Background technique
Flaxen fiber fiber is a kind of fiber ancient, graceful, with cultural connotation, in China's Nature Fibre Textile development history
On occupy important historical role.The special ultrafine micropore structure of flaxen fiber fibrous inside makes it have powerful adsorption capacity,
Formaldehyde in air, benzene, toluene, the harmful substances such as ammonia, elimination bad smell can be adsorbed.And flaxen fiber fiber contains a variety of natural bacteriostatics
Microelement can effectively inhibit bacterium acarid to breed, play the role of the anti-peculiar smell of antibacterial.It has been also equipped with superior simultaneously
Gas and conductivity of heat, it is pleasant that summer dress allows people to feel nice and cool, and winter then feels more comfortably, automatic constant-temperature, and people is allowed really to experience " natural
The glamour of air-conditioning " flaxen fiber.
Colloidal complex containing 25%--30% in flaxen fiber raw ramie (bast), is wrapped in the outside of flaxen fiber fiber,
Keep fiber mutually cemented together, cannot be directly used to weave, it is therefore necessary to carry out degumming process, release fiber and in " beam
Fiber " discrete state.
Traditional jute fiber degumming mainly with chemical Degumming is carried out based on caustic soda, boiled using strong acid, highly basic, high temperature and pressure
Refining is broken fiber molecule chain, and crystallinity improves, but fiber crops rigidity increases after chemical Degumming, and cohesive force is deteriorated, and fabric feeling is thick
Firmly, it takes scratchy.And the problems such as at high cost, seriously polluted existing for flaxen fiber chemical Degumming technology.On the other hand, in order to solve
Chemical Degumming is at high cost, the problems such as seriously polluted, and since the eighties, China takes some novel Degumming methods, such as enzyme process
Degumming and microbial degumming, although the problems such as both periods for solving chemical Degumming are short, seriously polluted.But enzyme process
The activity stability of enzyme is poor in Degumming method, degumming low efficiency；Its strain enzymatic productivity of microbial degumming is low, not only degumming efficiency
It is low, and Spawn incubation technical requirements are high, and both Degumming method degumming technologies are also immature, and from industrial production, there are also very big
Distance.
Summary of the invention
The purpose of the present invention is to provide a kind of Novel clean jute fiber degumming technology, this method is simple and easy, is conducive to
Industrial application.
One kind cleans degumming method of bast fiber, including degumming step, and cooking liquor used is fiber in the degumming step
The dilution of plain extractant, the raffinate of Liang containing Gu≤10%, except at least one of waste water after residue and lignin,
The extractant is mixed gained by A liquid with B liquid, wherein and the volume ratio of the A liquid and B liquid is 3:1~5:1,
The A liquid is grouped as by following groups by mass percentage: sodium hydroxide 8~12%；Sodium carbonate 1~3%；Chlorination
Sodium 2~4%；Calcium chloride 1~3%；Sodium phosphate 0.5~1%；Sodium formate 0.1~0.5%；Sodium metasilicate 2.2~4.2%；Sulfurous acid
Sodium 1~2%；Water 70.3~84.2%；
The B liquid is made of crude enzyme liquid and enzyme stabilizers, wherein the crude enzyme liquid is Bacillus subtillis (Bacillus
Subtilis after) CGMCC1.836 is fermented, the clear crude enzyme liquid that is obtained after separating thallus.
Bacillus subtillis described in the method for the invention (Bacillus subtilis) CGMCC1.836 can be general by China
Logical Microbiological Culture Collection administrative center (China General Microbiological Culture Collection
Center, CGMCC) it obtains.
" raffinate of Liang containing Gu≤10% " of the present invention refers to the resulting boiling raffinate after once boiling process.In boiling
In the process, once boiling is carried out to wood fibre using cooking liquor, carries out solid filtering after boiling, Liang containing Gu after filtering≤
10% cooking liquor is named as " raffinate of Liang containing Gu≤10% "
It is of the present invention to refer to resulting removing residue and wood after secondary boiled process " except the waste water after residue and lignin "
The waste water of element.During the cooking process, boiling twice is carried out to wood fibre using cooking liquor, after the second boiling in cooking liquor
Containing a large amount of residue and lignin solid, the cooking liquor after residue and lignin are removed is named as " except useless after residue and lignin
Water ".
In boiling step of the present invention cooking liquor used be the dilution of cellulose extractant, Liang containing Gu≤10% it is residual
Liquid, except one of waste water after residue and lignin or in which any two kinds or any three kinds of mixing.
Further, when the raffinate of dilution, Liang containing Gu≤10% that cooking liquor is cellulose extractant, except residue and wood
When being used in mixed way for two or three in the waste water after element, each component can be used in mixed way with arbitrary proportion, meet boiling needed for boiling
Bath raio.
The dilution of cellulose extractant of the present invention is preferably 10~20 times that extractant is diluted with water to original volume
Resulting solution.
Further, concentration of the enzyme stabilizers in B liquid is 4~6mM；Further, the preferably described enzyme is stablized
Concentration of the agent in B liquid is 5mM.
Cellulose extractant of the present invention, the preferably described A liquid are grouped as: hydroxide by mass percentage by following groups
Sodium 10%；Sodium carbonate 2%；Sodium chloride 3%；Calcium chloride 2.5%；Sodium phosphate 0.6%；Sodium formate 0.3%；Sodium metasilicate 3.1%；It is sub-
Sodium sulphate 1.4%；Water 77.1%.
Enzyme stabilizers of the present invention are polyol, preferably ethylene glycol or glycerol.
Further, the crude enzyme liquid is made as follows:
A. Bacillus subtillis (Bacillus subtilis) CGMCC1.836 is inoculated into seed culture medium, 36 DEG C
Shake culture 18h, is prepared into seed liquor；35L fermentation medium is prepared, is poured into 50L fermentor, it is cold after 121 DEG C of sterilizing 20min
But；According to 5% (v/v) inoculum concentration, seed liquor is connected in fermentation medium, 36 DEG C of cultures 60 h, ventilation quantity 15L/min,
Obtain fermentation liquid, wherein
The constituent of the seed culture medium is as follows: sucrose 20g/L, peptone 10g/L, corn pulp 30g/L, sulfuric acid
Ammonium 4g/L, dipotassium hydrogen phosphate 18.4g/L, potassium dihydrogen phosphate 6g/L, pH7.0；
The constituent of the fermentation medium is as follows: glucose 30g/L, corn pulp 15g/L, ammonium sulfate 4g/L, phosphoric acid
Hydrogen dipotassium 9.2g/L, potassium dihydrogen phosphate 3g/L, pH7.0；
B. the fermentation liquid obtained by step a is continuously accessed in tube centrifuge, revolution 14000rpm takes supernatant, i.e.,
For crude enzyme liquid.
Further, the B liquid is made as follows: being 4~6mM to thick by concentration of the enzyme stabilizers in B liquid
Enzyme stabilizers are added in enzyme solution
Further, the plant cellulose extractant that cleans is made as follows: B liquid is mixed with A liquid,
The volume ratio of middle A liquid and B liquid is 3:1~5:1.
Conditions of cooking in the step of preparation method preferred boiling of the present invention for cleaning flaxen fiber are as follows: boiling temperature
60~80 DEG C；Cooking pressure is normal pressure；70~120min of digestion time；Boiling bath raio (herein refers to fibrous raw material and cooking liquor
Mass ratio) it is 1:8~1:10.
A preferred technical solution of the invention are as follows:
One kind cleaning degumming method of bast fiber, and the method includes following processing steps:
Step 1: flaxen fiber is subjected to opening, impurity removal, and combing；
Step 2: the flaxen fiber tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio 1:8
~1:10；Normal pressure, 50 DEG C~80 DEG C of boiling temperature；40~80min of digestion time；
Step 3: the flaxen fiber after boiling being sent into washing step and is washed；
Step 4: being combed by flaxen fiber bleaching, the drying after cleaning, then through health, fining.
The invention has the benefit that the present invention is mainly used by Bacillus subtillis (Bacillus subtilis)
After CGMCC1.836 is fermented, the clear crude enzyme liquid that is obtained after separating thallus (main component be alkaline pectate lyase and
Alkaline polygalacturonase), sig water one bath in handle flaxen fiber so that the colloid in raw ramie occurs on molecular structure
Large change, the stability of colloidal complex are seriously destroyed, and are formed alternating and circulation that swelling-modification-removes colloid, are reached
The purpose of degumming is quickly carried out under normal temperature condition to flaxen fiber.
Specific embodiment
Following non-limiting embodiments can with a person of ordinary skill in the art will more fully understand the present invention, but not with
Any mode limits the present invention.
Test method described in following embodiments is unless otherwise specified conventional method；The reagent and material, such as
Without specified otherwise, commercially obtain.
It is of the present invention clean plant cellulose extractant (degumming agent), cooking liquor preparation and to clean flaxen fiber de-
Adhesive process method, includes the following steps:
1. cleaning the preparation of plant cellulose extractant (degumming agent), include the following steps:
A. Bacillus subtillis (Bacillus subtilis) CGMCC1.836 is inoculated into seed culture medium, 36 DEG C
Shake culture 18h, is prepared into seed liquor.35L fermentation medium is prepared, is poured into 50L fermentor, it is cold after 121 DEG C of sterilizing 20min
But.According to 5% inoculum concentration, seed liquor is connected in fermentation medium.36 DEG C of cultures 60h, ventilation quantity 15L/min.
The seed culture medium (g/L): sucrose 20, peptone 10, corn pulp 30, ammonium sulfate 4, dipotassium hydrogen phosphate 18.4,
Potassium dihydrogen phosphate 6, pH7.0.
The fermentation medium (g/L): glucose 30, corn pulp 15, ammonium sulfate 4, dipotassium hydrogen phosphate 9.2, biphosphate
Potassium 3, pH7.0.
B. the fermentation liquid obtained in step a is continuously accessed in tube centrifuge, revolution 14000rpm takes supernatant
Liquid can be made into crude enzyme liquid.
C. ethylene glycol (Dalian Pharmaceutical Group Hua Bo factory) stabilizer that concentration is 5mM is added in crude enzyme liquid, after mixing
B liquid, B liquid is mixed with A liquid, the volume ratio of A liquid and B liquid is respectively 3:1,4:1,5:1,
The A liquid is grouped as by following groups by mass percentage: sodium hydroxide 10%；Sodium carbonate 2%；Sodium chloride 3%；
Calcium chloride 2.5%；Sodium phosphate 0.6%；Sodium formate 0.3%；Sodium metasilicate 3.1%；Sodium sulfite 1.4%；Water 77.1%.
2. prepared by degumming cooking liquor, include the following steps: plant cellulose extractant (degumming agent) diluting 10~20 times
Prepare cooking liquor.
3. cleaning jute fiber degumming process, include the following steps:
Step 1: flaxen fiber is subjected to opening, impurity removal, and combing.
Step 2: the flaxen fiber tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio 1:8
~1:10；Normal pressure, 50 DEG C~80 DEG C of boiling temperature；40~80min of digestion time.
Step 3: the flaxen fiber after boiling being sent into washing step and is washed, the degumming impurity of attachment is washed away.
Step 4: being combed by flaxen fiber bleaching, the drying after cleaning, then through health, fining.
4. flaxen fiber before and after degumming is according to " FZ T 30001-1992 ramie in degumming method of bast fiber of the invention
Main chemical compositions systematic quantification analysis method " is detected:
(1) sample for weighing numb 4 parts of about 1.2g of sample, dry to constant weight in 105 ± 3 DEG C and (generally need 4h) drying weighing W0,
Sample is moved into 100mL triangular flask, 0.5%EDTA 60mL is added and is placed in autoclave, final vacuum of boiling
5min controls 105 ± 1 DEG C with adjustable transformer, extracts 45min.It takes out, is filtered with sand core funnel, retain filter residue, in 105 ± 3
It DEG C dries to constant weight and (generally needs 4h), cooling weighing W1, being accurate to 0.0001g pectin weight is W1-W0- W sand core.
(2) above-mentioned gained filter residue is moved into triangular flask in sand core funnel with glass bar and small brushes, is added
2moL/L hydrochloric acid 60mL, which is placed in autoclave, extracts hemicellulose, extracts 60min, and cold water is cooling, uses sand core immediately
Funnel filtering, hot distilled water wash filter residue 4~5 times, retain filter residue, dry to constant weight in 105 ± 3 DEG C and (generally need 4h), cooling to claim
Weight is accurate to 0.0001g, drying weighing W2, hemicellulose is W containing weight2-W1。
(3) above-mentioned gained filter residue is moved into 150mL beaker in tall form in sand core funnel, is added and is cooled to 10~15 in advance
DEG C 72% sulfuric acid 15mL be placed in refrigerator after 10~15 DEG C of reaction 4h, with glass bar extruding make homogenate, every 30min mono-
It is secondary, totally 2~3 times, distilled water 100mL is added to stand overnight, is filtered with the sand core funnel of known weight, thermal distillation is washed 5~6 times,
Retain filter residue, dry to constant weight in 105 ± 3 DEG C and (generally need 4h), cooling weighing is accurate to 0.0001g, drying weighing W3, fiber
Plain weight is W3-W2。
(4) lignin weight is W3-WSand core。
CX--- pectin content；Hemicellulose level；Content of cellulose；Content of lignin.
WX--- pectin weight；Hemicellulose weight；Cellulose；Lignin weight (g).
W0--- sample dry weight (g).
1 plant cellulose extractant of embodiment is applied in hemp fiber degumming engineering
1. cooking liquor is prepared: cellulose extractant, which is prepared, is --- A liquid is 5:1 with B liquid volume ratio；The ratio of 1:10 is pressed again
Example is diluted with water, is configured to cooking liquor.
2. technique: combing removal of impurities hemp raw material 5kg；Bath raio is 1:10；Boiling 50min after addition cooking liquor；Boiling
Temperature is 80 DEG C；Hemp composition detection through washing, bleaching, drying, health, fining combing front and back the results are shown in Table 1, tool
Body are as follows:
Step 1: hemp is subjected to opening, impurity removal, and combing.
Step 2: the flaxen fiber 5kg tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio
1:10；Normal pressure, 80 DEG C of boiling temperature；Digestion time 50min.
Step 3: the hemp after boiling being sent into washing step and is washed, the degumming impurity of attachment is washed away.
Step 4: being combed by hemp bleaching, the drying after cleaning, then through health, fining.
Embodiment 2
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 1
It for a part of cooking liquor, is required according to bath raio, cooking liquor (i.e. the A liquid and B liquid that cooking liquor insufficient section is prepared with embodiment 1
Volume ratio is 5:1；It is diluted again in the ratio of 1:10 with water, the cooking liquor being formulated) supplemented (boiling newly prepared
Liquid accounts for the 30% of whole cooking liquors).
2. technique the results are shown in Table 1 with embodiment 1, hemp composition detection.
Embodiment 3
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 2
It for a part of cooking liquor, is required according to bath raio, cooking liquor insufficient section is supplemented (new with the cooking liquor that embodiment 1 is prepared
The cooking liquor of preparation accounts for the 30% of whole cooking liquors).
2. technique the results are shown in Table 1 with embodiment 1, hemp composition detection.
To embodiment 1, embodiment 2,3 boiling of embodiment hemp degumming effect --- i.e. fiber Indexs measure
(cellulose, hemicellulose, pectin, lignin, fineness, strength) is shown in Table 1.As seen from Table 1, content of cellulose greatly improves；
Lignin is effectively reduced with pectin；Fineness strongly reaches the requirement that can spin high branch mixed yarn.The cooking liquor of this patent development can
To apply in hemp fiber degumming very well.
1 hemp testing result of table
4 plant cellulose extractant of embodiment is applied in hemp fiber degumming engineering
1. cooking liquor is prepared: cellulose extractant, which is prepared, is --- A liquid is 4:1 with B liquid volume ratio；Again in the ratio of 1:15
The waste water obtained by 1 step 3 of embodiment washing hemp is diluted (waste water be handled through the way of distillation obtained by), is configured to
Cooking liquor.
2. technique: combing removal of impurities hemp raw material 5kg；Bath raio is 1:10；Boiling 50min after addition cooking liquor；Reaction
Temperature is 80 DEG C；Hemp Indexs measure (i.e. cellulose, half fiber after washing, bleaching, drying, health, fining combing
Dimension element, pectin, lignin, fineness, strength) it the results are shown in Table 2, specifically:
Step 1: hemp is subjected to opening, impurity removal, and combing.
Step 2: the flaxen fiber 5kg tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio
1:10；Normal pressure, 80 DEG C of boiling temperature；Digestion time 50min.
Step 3: the hemp after boiling being sent into washing step and is washed, the degumming impurity of attachment is washed away.
Step 4: being combed by hemp bleaching, the drying after cleaning, then through health, fining.
Embodiment 5
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 4
It for a part of cooking liquor, is required according to bath raio, cooking liquor insufficient section is supplemented (new with the cooking liquor that embodiment 4 is prepared
The cooking liquor of preparation accounts for the 30% of whole cooking liquors).
2. technique is shown in Table 2 with embodiment 4, hemp testing result.
Embodiment 6
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 5
It for a part of cooking liquor, is required according to bath raio, cooking liquor insufficient section is supplemented (new with the cooking liquor that embodiment 4 is prepared
The cooking liquor of preparation accounts for the 30% of whole cooking liquors).
2. technique is shown in Table 2 with embodiment 4, hemp testing result.
To embodiment 4, embodiment 5,6 boiling of embodiment flaxen fiber degumming effect --- i.e. fiber Indexs measure is shown in Table
2.As seen from Table 2, cooking liquor concentration reduces, and thinner ratio increases, and after digestion time reduces, repeats the hemp of boiling three times
Its cellulose, which extracts (degumming) effect, still higher level, and lignin is effectively reduced with pectin；Fineness, strongly reach can
Spin the requirement of high branch mixed yarn.The plant cellulose extractant of this patent development can be applied in hemp fiber degumming very well.
2 hemp testing result of table
7 plant cellulose extractant of embodiment is applied in ramee degumming
1. cooking liquor is prepared: cellulose extractant, which is prepared, is --- A liquid is 4:1 with B liquid volume ratio；Again in the ratio of 1:15
It is diluted with water, is configured to cooking liquor.
2. technique: shredding removal of impurities ramee raw material 5kg；Bath raio is 1:10；Digestion time 50min；Boiling temperature is 80
℃；Ramee composition detection the results are shown in Table 3 after washing, specifically:
Step 1: ramee is subjected to opening, impurity removal, and combing.
Step 2: the ramee 5kg tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio
1:10；Normal pressure, 80 DEG C of boiling temperature；Digestion time 50min.
Step 3: the ramee after boiling being sent into washing step and is washed, the degumming impurity of attachment is washed away.
Step 4: being combed by ramee bleaching, the drying after cleaning, then through health, fining.
Embodiment 8
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 7
It for a part of cooking liquor, is required according to bath raio, cooking liquor insufficient section is supplemented (new with the cooking liquor that embodiment 7 is prepared
The cooking liquor of preparation accounts for the 30% of whole cooking liquors).
2. technique the results are shown in Table 3 with embodiment 7, ramee composition detection.
Embodiment 9
1. cooking liquor is prepared: the resulting whole boiling raffinates (raffinate of Liang containing Gu≤10%) of step 2 are made in embodiment 8
It for a part of cooking liquor, is required according to bath raio, cooking liquor insufficient section is supplemented (new with the cooking liquor that embodiment 7 is prepared
The cooking liquor of preparation accounts for the 30% of whole cooking liquors).
2. technique the results are shown in Table 3 with embodiment 7, ramee composition detection.
To embodiment 7, embodiment 8,9 boiling of embodiment ramee glue effect --- i.e. fibre composition detection is (fine
Dimension element, hemicellulose, pectin, lignin) it is shown in Table 3.As seen from Table 3, the extractant (degumming agent) of this patent development is in ramie fibre
It ties up in degumming, has also reached fine degumming effect.
3 ramee testing result of table
Claims (10)
1. one kind cleans degumming method of bast fiber, it is characterised in that: including degumming step, boiling used in the degumming step
Liquid be cellulose extractant dilution or cooking liquor be cellulose extractant dilution and Liang containing Gu≤10% raffinate,
Except the combination of at least one of waste water after residue and lignin,
The extractant is mixed gained by A liquid with B liquid, wherein and the volume ratio of the A liquid and B liquid is 3:1~5:1,
The A liquid is grouped as by following groups by mass percentage: sodium hydroxide 8~12%；Sodium carbonate 1~3%；Sodium chloride 2
~4%；Calcium chloride 1~3%；Sodium phosphate 0.5~1%；Sodium formate 0.1~0.5%；Sodium metasilicate 2.2~4.2%；Sodium sulfite 1
~2%；Water 70.3~84.2%；
The B liquid is made of crude enzyme liquid and enzyme stabilizers, wherein the crude enzyme liquid is Bacillus subtillis (Bacillus
Subtilis after) CGMCC1.836 is fermented, the clear crude enzyme liquid that is obtained after separating thallus.
2. according to the method described in claim 1, it is characterized by: concentration of the enzyme stabilizers in B liquid is 4~6mM.
3. according to the method described in claim 2, it is characterized by: concentration of the enzyme stabilizers in B liquid is 5mM.
4. method according to claim 1 or 2, it is characterised in that: the enzyme stabilizers are ethylene glycol or glycerol.
5. according to the method described in claim 1, it is characterized by: the A liquid is grouped as by following groups by mass percentage:
Sodium hydroxide 10%；Sodium carbonate 2%；Sodium chloride 3%；Calcium chloride 2.5%；Sodium phosphate 0.6%；Sodium formate 0.3%；Sodium metasilicate
3.1%；Sodium sulfite 1.4%；Water 77.1%.
6. according to the method described in claim 2, it is characterized by: the B liquid is made as follows:
A. Bacillus subtillis (Bacillus subtilis) CGMCC1.836 is inoculated into seed culture medium, 36 DEG C of concussions
18h is cultivated, seed liquor is prepared into；35L fermentation medium is prepared, is poured into 50L fermentor, it is cooling after 121 DEG C of sterilizing 20min；
According to 5% inoculum concentration, seed liquor is connected in fermentation medium, 36 DEG C of cultures 60h, ventilation quantity 15L/min obtain fermentation liquid,
Wherein,
The constituent of the seed culture medium is as follows: sucrose 20g/L, peptone 10g/L, corn pulp 30g/L, ammonium sulfate
4g/L, dipotassium hydrogen phosphate 18.4g/L, potassium dihydrogen phosphate 6g/L, pH7.0；
The constituent of the fermentation medium is as follows: glucose 30g/L, corn pulp 15g/L, ammonium sulfate 4g/L, phosphoric acid hydrogen two
Potassium 9.2g/L, potassium dihydrogen phosphate 3g/L, pH7.0；
B. the fermentation liquid obtained by step a is continuously accessed in tube centrifuge, revolution 14000rpm takes supernatant, as slightly
Enzyme solution is that enzyme stabilizers are added into crude enzyme liquid by 4~6mM by concentration of the enzyme stabilizers in B liquid.
7. according to the method described in claim 1, it is characterized by: the dilution of the cellulose extractant is extractant water
It is diluted to 10~20 times of resulting solution of original volume.
8. according to the method described in claim 7, it is characterized by: conditions of cooking in the step of degumming are as follows: boiling temperature 50~
80℃；Cooking pressure is normal pressure；40~80min of digestion time；Boiling bath raio is 1:8~1:10.
9. according to the method described in claim 1, it is characterized by: when the dilution that cooking liquor is cellulose extractant and containing solid
The raffinate of Liang≤10%, except at least one of waste water after residue and lignin combination when, each component is mixed with arbitrary proportion.
10. according to the method described in claim 1, it is characterized by: the method includes following processing steps:
Step 1: flaxen fiber is subjected to opening, impurity removal, and combing；
Step 2: the flaxen fiber tentatively combed being put into digester, and the cooking liquor prepared is added；Boiling bath raio 1:8~1:
10；Normal pressure, 50 DEG C~80 DEG C of boiling temperature；40~80min of digestion time；
Step 3: the flaxen fiber after boiling being sent into washing step and is washed；
Step 4: being combed by flaxen fiber bleaching, the drying after cleaning, then through health, fining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710533841.2A CN107326453B (en) | 2017-07-03 | 2017-07-03 | One kind cleaning degumming method of bast fiber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710533841.2A CN107326453B (en) | 2017-07-03 | 2017-07-03 | One kind cleaning degumming method of bast fiber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107326453A CN107326453A (en) | 2017-11-07 |
| CN107326453B true CN107326453B (en) | 2019-06-04 |
Family
ID=60198549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710533841.2A Active CN107326453B (en) | 2017-07-03 | 2017-07-03 | One kind cleaning degumming method of bast fiber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107326453B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102185434B1 (en) * | 2019-07-30 | 2020-12-01 | 권민정 | A method of producing eco-friendly hemp fibers including enzymatic treatment step |
| CN111100765A (en) * | 2019-12-23 | 2020-05-05 | 张艳 | Preparation method of degumming agent |
| CN112981551A (en) * | 2021-02-25 | 2021-06-18 | 阿勒泰戈宝茶股份有限公司 | Method for degumming apocynum skin |
| CN113718541B (en) * | 2021-07-13 | 2022-10-21 | 大连工业大学 | Preparation method of intrinsic hydrophobic oleophylic nanocellulose |
| CN113388896B (en) * | 2021-07-13 | 2022-08-02 | 基准(北京)生物技术研究院有限公司 | Preparation method of hydrophobic oleophylic nanocellulose based on immobilized enzyme chemistry-oxidative degumming |
| CN114182361A (en) * | 2021-11-08 | 2022-03-15 | 宜兴市舜昌亚麻纺织有限公司 | Composite biological enzyme degumming agent suitable for flax fiber supercritical degumming |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101260568A (en) * | 2008-04-01 | 2008-09-10 | 张明芮 | Method for preparing natural fiber by okra straw |
| CN103451745A (en) * | 2013-08-12 | 2013-12-18 | 六安市凯旋大麻纺织有限责任公司 | Hemp biological degumming process |
| CN106164237A (en) * | 2014-04-11 | 2016-11-23 | 诺维信公司 | Composition of detergent |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463503A (en) * | 2009-01-15 | 2009-06-24 | 安徽农业大学 | Ramie compositional biological enzyme degumming technological process |
| CN104630910B (en) * | 2015-02-15 | 2016-08-31 | 东华大学 | A kind of method that candida tropicalis DK2 bacterial strain and hydrogen peroxide combined production process prepare flaxen fiber |
| CN104674354B (en) * | 2015-02-15 | 2016-09-07 | 东华大学 | A kind of candida tropicalis DK2 bacterial strain and the ultrasonic combined method preparing flaxen fiber of hydrogen peroxide |
| CN104630909B (en) * | 2015-02-15 | 2016-08-17 | 东华大学 | A kind of volume branch Mucor DK1 bacterial strain and the ultrasonic combined method preparing flaxen fiber of hydrogen peroxide |
| CN104630908B (en) * | 2015-02-15 | 2016-09-07 | 东华大学 | A kind of method that volume branch Mucor DK1 bacterial strain and hydrogen peroxide combined production process prepare flaxen fiber |
-
2017
- 2017-07-03 CN CN201710533841.2A patent/CN107326453B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101260568A (en) * | 2008-04-01 | 2008-09-10 | 张明芮 | Method for preparing natural fiber by okra straw |
| CN103451745A (en) * | 2013-08-12 | 2013-12-18 | 六安市凯旋大麻纺织有限责任公司 | Hemp biological degumming process |
| CN106164237A (en) * | 2014-04-11 | 2016-11-23 | 诺维信公司 | Composition of detergent |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107326453A (en) | 2017-11-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107326453B (en) | One kind cleaning degumming method of bast fiber | |
| CN100393924C (en) | Extraction and preparation method of fibrilia, fibrilia obtained by the method and application thereof | |
| CN100529201C (en) | Microbiological degumming method for mulberry fibre | |
| CN102492997B (en) | Flash explosion and base boiling combined degumming method of natural bamboo fiber | |
| CN102206873A (en) | Process for producing hemp-like bamboo fibers | |
| CN104032380B (en) | A kind of preparation method of mulberry fibre | |
| CN101245492A (en) | Marijuana second hard fibre processing method | |
| CN103266358B (en) | Method for degumming ramie | |
| CN103397391B (en) | A kind of biological enzyme degumming method of linen fibre | |
| CN101649494A (en) | Novel biological degumming agent for ramie raw materials | |
| CN104099670A (en) | Method for degelatinizing hemp fiber with biological enzyme degelatinizing method combined with chemical degelatinizing method | |
| CN107287972B (en) | A kind of preparation method cleaning reed pulp | |
| CN102943403B (en) | A kind of Wood pomace modified producing technology for the production of viscose | |
| CN110528102A (en) | A kind of cellulose viscose manufacturing technique based on hemp dissolving pulp | |
| CN107699596B (en) | One kind cleaning plant cellulose extractant and its preparation method and application | |
| CN106319708A (en) | Biological enzyme type preparation method of flax fiber and tencel blended yarn | |
| CN103498381A (en) | Pineapple fiber pulp and preparation method thereof | |
| CN101805725B (en) | Complex enzyme generated by aspergillus niger and application thereof in boiling-off of flax roving | |
| CN111394818B (en) | Liquorice regenerated cellulose fiber and preparation method thereof | |
| CN105239233B (en) | A kind of antibacterial mulberry fibre silk/cotton blended yarn | |
| CN100460574C (en) | Process for decompressing and debonding cannabis | |
| CN100535202C (en) | Microbiological degumming method for smartweed fibre | |
| CN103103747A (en) | Pretreatment method for pineapple fibers | |
| CN107419355B (en) | A kind of processing method of useless acrylic fibres cloth | |
| CN102242405A (en) | Method for extracting abelmoschus fibers from abelmoschus plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |