JP2009232813A - Method for producing bacterial cellulose, and culture medium for production - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a culture medium capable of effectively utilizing apple marc, and inexpensively producing bacterial cellulose with cellulose-producing bacteria. <P>SOLUTION: An enzymic saccharified liquid (first saccharified liquid) 10 is obtained from a first waste 1 which can be a nitrogen source by an enzymic saccharification treating process P1, and an enzymic saccharified liquid (second saccharified liquid) 20 is obtained from a second waste 2 by an enzymic saccharification treating process P2, respectively. The first saccharified liquid 10 is mixed with the second saccharified liquid 20 by a mixing treatment process P3. A necessary post-treatment such as a pH adjusting treatment process P4 is carried out therefor to compose the culture medium 100. Bean-curd refuse can be used as the first waste, and other vegetable wastes, e.g. the apple marc can be used as the second waste. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a method for producing bacterial cellulose and a production medium, and in particular, provides a medium capable of producing bacterial cellulose at low cost using a cellulose-producing bacterium and production conditions thereof.

  The fact that microorganisms produce cellulose was found more than 100 years ago by A. J. et al. Reported by Brown. Such cellulose produced by microorganisms is generally called bacterial cellulose or biocellulose (hereinafter referred to as “bacterial cellulose” or “BC”). The so-called “Nata de Coco”, which became a big boom in Japan around 1992, is a dessert ingredient that is popular for its unique texture, but it is the bacteria produced by the microorganisms that produce its elasticity. Cellulose.

  Bacterial cellulose is known to have excellent properties not found in plant-derived cellulose. For example, plant cellulose contains lignin, hemicellulose, etc., whereas bacterial cellulose is pure cellulose, and the fiber width of bacterial cellulose is said to be 1/100 to 1/1000 compared to plant cellulose. There are high added-value characteristics as a substance, such as being so thin, having a high Young's modulus due to the strength of hydrogen bonds and plane orientation, and forming a liquid crystal.

  Various utilization researches have been promoted by utilizing such characteristics of bacterial cellulose. For example, use for fuel cell electrolyte membranes (Mai Mukai et al .: Abstracts of the 13th Annual Conference of Cellulose Society, p73, 2006), measurement devices for cancer diagnosis, and UV protection cosmetics (Ri Tanabe: Chemistry and Biology, 45, p600-601, 2007), etc., for industrial and medical fields.

  As an example in which bacterial cellulose is actually commercialized, there is a case where it is used for an acoustic diaphragm such as a speaker (Japanese Patent No. 2953743). In addition, development of a culture medium for improving the production amount of bacterial cellulose has been promoted conventionally, and many technical proposals have been made (Japanese Patent No. 2766165, Japanese Patent No. 2816939, Japanese Patent No. 3809551, Japanese Patent Laid-Open No. Hei 5- 1718, JP-A-7-184675, JP-A-7-184777, JP-A-8-34802, JP-A-9-296003, JP-A-10-146198, JP-A-11-181001, JP-A-2000-4895, JP-A-2005-80571) .

  However, in order to promote the practical application of utilization research as described above, it is an essential condition to enable low-cost production of bacterial cellulose. From this point of view, development of bacterial cellulose production technology using food processing waste is a significant theme, but there are still few research cases. However, until now, bacterial cellulose production using glucose obtained by enzymatic saccharification of beet pulp (Non-patent Document 1), hot water extract of banana peel, onion, carrot, melon peel, and juice of watermelon peel Techniques such as bacterial cellulose production (Non-patent Document 2), and mushroom production and bacterial cellulose production from water-soluble sugar remaining in processing waste (Non-patent Document 3) are disclosed.

Kazutoshi Watanabe et al .: Research on biosynthesis and utilization technology of high-functional polysaccharides by using agricultural processed waste, etc. Fiscal 1997 Science and Technology Agency commissioned research report, p18-21, 1998 Yuichi Shimizu et al .: Bulletin of Tomakomai National College of Technology, No. 37, p127-134, 2002 Yu Tamai: Creation of artificial cell wall imitating cell wall construction process 2003-2005, p105-129, 2006

  Now, the present inventors have so far made effective use of apple pomace, which is discharged in large quantities from apple processing plants and processed at a high cost during fruit production. In order to reduce production costs, we have been working on bacterial cellulose production tests using apple juice residue as a sugar resource. Among them, a production test was carried out using a saccharified solution obtained by saccharifying apple juice residue with an enzyme as a medium. However, in order to obtain good production, it is necessary to add fermentation nutrients such as yeast extract separately. Eventually, it was difficult to produce bacterial cellulose with apple juice residue alone (Satoshi Takahashi: Food Chemical, 21, p29-31, 2005; Jun Takahashi: Fruit Juice Association Bulletin, 558, p1-9, 2005)

  That is, it is considered that the amount of nitrogen as a fermentation nutrient source is insufficient only with apple juice residue, and it has been necessary to compensate for this by some method. That is, the problem to be solved by the present invention is to provide a medium capable of effectively producing bacterial cellulose by cellulose-producing bacteria, and a method for producing bacterial cellulose using the apple juice residue effectively and inexpensively. It is to be.

  Moreover, the problem to be solved by the present invention is that not only apple juice residue but also effective use of plant wastes with low nitrogen content and good production of bacterial cellulose by cellulose-producing bacteria at low cost It is to provide a medium and a method for producing bacterial cellulose thereby.

  As a result of diligent study to solve the above problems, the present inventor saccharified apple juice residue and okara discharged as a food processing residue by enzymatic treatment, and the saccharified solution alone or mixed for cellulose production. It has been found that bacterial cellulose can be produced at low cost by culturing cellulose-producing bacteria as a medium. In other words, the present invention, which is a means for solving this problem, has been achieved by utilizing Okara, which is also a plant waste, as a nitrogen source that can sufficiently reduce the production cost of bacterial cellulose, which is the intended purpose. Using okara, which is a protein-rich waste discharged when producing tofu and soy milk from soybeans, is very effective not only in supplementing the nitrogen source but also in reducing the medium cost.

That is, the invention claimed or at least disclosed in the present application is as follows.
(1) A medium for producing bacterial cellulose, which is a medium for producing bacterial cellulose, which contains vegetable waste that can be enzymatically saccharified and also serves as a nitrogen source as a raw material.
(2) Bacterial cellulose production medium that is a vegetable waste that can be enzymatically saccharified and also serves as a nitrogen source (hereinafter referred to as "first waste"), and another vegetable waste that can be enzymatically saccharified ( Hereinafter, a culture medium for producing bacterial cellulose using “second waste” as a raw material.
(3) The medium for producing bacterial cellulose according to (1) or (2), wherein the first waste is Okara.
(4) The culture medium for producing bacterial cellulose according to (2), wherein the first waste is Okara, and the second waste is a juice residue from a plant edible part.
(5) The culture medium for producing bacterial cellulose according to (2), wherein the first waste is Okara and the second waste is apple fruit juice residue.

(6) An enzyme saccharified solution (hereinafter referred to as “first saccharified solution”) is obtained from the first waste, and an enzyme saccharified solution (hereinafter referred to as “second saccharified solution”) is obtained from the second waste. The medium for producing bacterial cellulose according to any one of (2), (4) and (5), wherein the medium is prepared by mixing the first saccharified solution and the second saccharified solution.
(7) The culture medium for producing bacterial cellulose according to (6), wherein the first saccharified solution is used in an amount of 25% by weight or more of the second saccharified solution.
(8) Enzymatic saccharification liquid is manufactured from the mixture of said 1st waste and said 2nd waste, The bacterial cellulose manufacture in any one of (2), (4) or (5) characterized by the above-mentioned Culture medium.
(9) A method for producing bacterial cellulose, comprising culturing cellulose-producing bacteria using the medium for producing bacterial cellulose according to any one of (1) to (8) to obtain bacterial cellulose.

  Since the bacterial cellulose production method and production medium of the present invention are configured as described above, according to this, the apple juice residue or other plant waste having a low nitrogen content can be used effectively, Bacterial cellulose by cellulose-producing bacteria can be produced satisfactorily.

  Especially when using the apple juice residue, the present invention uses the apple juice residue discharged from the apple processing plant and the okara discharged from the tofu processing plant as a medium for producing bacterial cellulose. Bacterial cellulose can be produced at a low cost, and environmental waste can be reduced by reusing industrial waste.

  Furthermore, in the present invention, cellulase is used as a saccharifying enzyme, and the plant cell wall is enzymatically decomposed into low molecular weight carbohydrates that can be assimilated by microorganisms. Unlike the acid decomposition method, the subsequent enzyme treatment method eliminates the need for subsequent waste liquid treatment, and at the same time reduces the amount of waste solids, thereby reducing the environmental burden. Such special processes and equipment are also unnecessary.

Hereinafter, the present invention will be described in more detail.
The medium for producing bacterial cellulose of the present invention can use vegetable waste (first waste) that can be enzymatically saccharified and also serves as a nitrogen source as a raw material, or in addition to the first waste, another enzymatic saccharification is possible. The basic constitution is to use a simple vegetable waste (second waste).

  The first waste is required to be capable of enzymatic saccharification and to be a nitrogen source. Okara, which is waste generated in the process of producing tofu and soymilk, can be preferably used. Since the first waste is saccharified by enzymatic treatment, it can serve as a saccharide source and a nitrogen source, and can be used alone as a raw material for bacterial cellulose. In addition, as the first waste of the present invention, the oil residue of oil seeds such as sesame, rapeseed, safflower, and sunflower is also a nitrogen source, and therefore satisfies the requirements.

  The second waste is required to be capable of enzymatic saccharification. Even when the second waste does not become a good nitrogen source, by using the first waste in combination, good bacterial cellulose production by the cellulose-producing bacteria can be obtained, and the waste medium can be used as an inexpensive medium. it can.

  As the second waste, for example, apple juice residue can be suitably used. As described above, it is difficult to obtain a bacterial cellulose production medium with good growth by using apple juice residue alone, but by using it together with the first waste that can be a nitrogen source, apple juice residue can be used as a medium raw material. It is fully possible to do. In addition, as a 2nd waste of this invention, the squeeze residue from a plant body edible part other than an apple squeeze residue, and other vegetable waste also correspond. In other words, waste that can be enzymatically saccharified, in other words, can be a source of sugar, can be produced from food processing processes, can be produced from processing processes other than food processing, or can be produced, harvested, shipped What is produced from processes other than time processing also falls under the second waste of the present invention.

  Here, the plant edible part refers to an edible part in a plant that becomes edible, such as fruits, vegetables, cereals, beans, wild vegetables and the like. Especially for fruits, apples, pears, peaches, grapes, mandarin oranges, natsumikan, other citrus fruits, pears, oysters, chestnuts, sweet potatoes, umes, bananas, pineapples, blueberries, cassis, kiwi fruits and other fruits, and vegetables, strawberry・ Fruit vegetables such as watermelon and melon, fruits and vegetables such as tomatoes, pumpkins, edamame, sweet corn, leafy vegetables such as spinach, garlic, onion, carrots, beets, burdock, Chinese yams, other yam genus, yacons, etc. Each edible part in root vegetables, spicy vegetables such as ginger, etc.

  In addition, waste other than the squeezed residue in the edible part of the plant corresponds to the second waste of the present invention as long as it can be a sugar source. For example, apple, pineapple core / skin separated from edible part, chestnut, mandarin, banana, melon, watermelon, pumpkin, potato skin, carrot, burdock, radish leaf / skin, sweet corn skin / core, etc. Widely applicable. It also includes non-edible plant waste. For example, flowers, leaves, stems, roots, etc. In addition, processing waste derived from fungi such as waste generated during mushroom production and processing also falls under this category.

  FIG. 1 is a basic flow diagram showing an example of a method for producing a medium for producing bacterial cellulose of the present invention. As shown in the figure, when a medium is prepared and manufactured using both the first waste and the second waste, the enzyme saccharified solution (first saccharified solution) 10 is prepared from the first waste 1 by the enzyme saccharification treatment process P1. In addition, the enzymatic saccharification liquid (second saccharification liquid) 20 is obtained from the second waste 2 by the enzymatic saccharification treatment process P2, and the first saccharification liquid 10 and the second saccharification liquid 20 are mixed by the mixing treatment process P3. The medium 100 can be configured by performing necessary post-treatments such as pH adjustment treatment step P4.

  FIG. 1-2 is a flowchart showing in detail the process for producing bacterial cellulose in the examples described in detail later (“Sumiteam” is a registered trademark in the figure).

On the other hand, the medium can be produced by a different method.
FIG. 2 is a basic flow diagram showing another example of the method for producing a medium for producing bacterial cellulose of the present invention. As shown in the figure, the first waste 1 and the second waste 2 are pretreated as necessary and then mixed in the mixing process P21, and the resulting mixture 23 is subjected to the enzymatic saccharification process P22. The enzyme saccharified solution 24 may be obtained, and necessary post-treatment such as a pH adjustment process P23 may be performed on the enzyme saccharified solution 24 to constitute the culture medium 200.

FIG. 2-2 is a flowchart showing an example of a bacterial cellulose production process based on the basic flow shown in FIG. 2 (“Sumiteam” in the figure is a registered trademark). Also,
FIG. 2-3 is a graph showing the amount of bacterial cellulose produced by the mixing ratio of the apple juice residue saccharified solution and the okara saccharified solution in the production method shown in FIG. As shown in the drawings, a certain bacterial cellulose production can be obtained even by the manufacturing method shown in FIG.

  In particular, the culture medium for producing bacterial cellulose of the present invention can use okara as the first waste and apple fruit juice residue as the second waste. Hereinafter, the present invention will be described in more detail with reference to such examples, but the present invention is not limited thereto.

  First, the production amount of bacterial cellulose is increased by using a kind of acetic acid bacteria, Gluconacetobacter xylinus, preferably Gluconacetobacter xylinus NBRC16682 strain, as a cellulose-producing bacterium.

  Bacterial cellulose-producing bacteria are pre-cultured in a liquid medium and used after increasing the number of bacteria. The medium used at this time is the National Institute for Product Evaluation and Technology (NITE), a distributor of the Gluconacetobacter xylinus NBRC16682 strain. Medium No. 4 composed of polypeptone, yeast extract, glucose, mannitol, magnesium sulfate heptahydrate and ethanol recommended in (1). 350 (Table 1) could be used suitably. However, it is not limited to this, and any medium may be used as long as the enrichment of cellulose-producing bacteria can be confirmed.

  MediumNo. 350 was used as a medium, and a culture solution obtained by shaking and culturing Gluconacetobacter xylinus NBRC16682 strain at 30 ° C. was used as an inoculum for bacterial cellulose production.

  In the Gluconacetobacter xylinus NBRC16682 strain used as a test strain in the present invention, Medium No. When 350 was used as the medium, the production of bacterial cellulose was confirmed in the pH range of 3 to 7, and the production amount was particularly increased in the range of pH 5 to 7.

  On the other hand, in order to use apple juice residue and okara as a liquid medium, as an enzyme treatment pretreatment, an equal amount of water is added to apple juice residue in a wet weight ratio, and to okara in a wet weight ratio. After adding 4 times the amount of water, the mixture was pulverized with a mixer and then autoclaved (121 ° C., 20 min). Thereafter, 0.5 (w / w)% of the cellulase-based saccharifying enzyme was added at a residual wet weight ratio, and the mixture was shaken at 40 ° C. The centrifuged supernatant was filtered to recover the saccharified solution. By this operation, liquefaction progressed and cell wall polysaccharides were enzymatically decomposed, so that the volume of apple juice residue and okara was reduced, and a saccharified solution in which water-soluble sugars such as glucose were gradually increased could be recovered.

  Thereafter, the saccharified solution is mixed at an arbitrary ratio, the pH is adjusted to 5 to 7, and after sterilization, the inoculum is inoculated in an amount equivalent to 2% of the medium, followed by stationary culture at 30 ° C. for 1 week to produce bacterial cellulose. went. Cellulose-producing bacteria produced sugar outside the cells using saccharides, nitrogen sources and other inorganic components as nutrients, and gradually formed white bacterial cellulose from the surface of the medium.

  Since the obtained bacterial cellulose contains medium components and cells, the purified bacterial cellulose is removed by removing impurities with, for example, an aqueous sodium dodecyl sulfate (SDS) solution or an aqueous sodium hydroxide (NaOH) solution. I was able to get it. As a result, a mixture of saccharified liquid from apple juice residue (hereinafter referred to as “apple juice residue saccharified liquid” or “apple residue saccharified liquid”) and saccharified liquid from okara (hereinafter referred to as “okara saccharified liquid”) is mixed. Production increased from a ratio of 3: 1 or higher, and production was confirmed even in a medium containing 100% of okara saccharified solution. The mixing ratio of the two is preferably in the vicinity of 1: 1 to 1: 2, and at this time, the production amount of bacterial cellulose was maximized. From this, the first saccharified solution (here, the enzyme saccharified solution derived from Okara) is used as a guideline for producing a medium by using 25% by weight or more of the second saccharified solution (also the enzyme saccharified solution derived from apple juice residue). It is thought that it can be.

<Selection of cellulose-producing bacteria>
In order to improve the production amount of bacterial cellulose, it is essential to select cellulose-producing bacteria having high productivity. Accordingly, the cellulosic bacteria shown in Table 2 were used as test strains, and 2 platinum ears from the slant slant that had been refrigerated and stored were each medium no. 350 (20 ml) was inoculated and cultured with shaking at 30 ° C. for 3 days (preculture solution). Separately prepared Medium No. 350 was adjusted to pH 3, 5, 7, 9, 11 and sterilized to obtain a BC production medium (5 ml was dispensed into a sterile petri dish). This was inoculated with 100 μl of the preculture, and allowed to stand at 30 ° C. for 1 week.

One week later, the produced bacterial cellulose is recovered, washed repeatedly with 2% SDS aqueous solution or 4% NaOH aqueous solution, the impregnated medium and cells are removed, filtered through a glass filter, washed with water, and the dry weight is measured. did.
Table 3 shows the average value of bacterial cellulose production for each pH by each test strain (unit of bacterial cellulose production: mg). In the table, “BC” refers to bacterial cellulose. The following charts are also the same. FIG. 3 is a graph of Table 3. As shown in these figures, the NBRC16682 strain (the fifth strain in the table) had the highest production amount of bacterial cellulose, and high production was confirmed in the pH range of 5-7.

<Pre-culture of cellulose-producing bacteria>
Subsequently, preculture of the production bacteria used in the bacterial cellulose production test was performed. Here, Gluconacetobacter xylinus NBRC16682 strain and Gluconacetobacter xylinus used in past tests (Takahashi Kaoru: Food Chemical, 21, p29-31, 2005; Takahashi Kaoru: Fruit Juice Association Bulletin, 558, p1-9, 2005) NBRC13693 strain (conventional strain) was used as a test strain, and 2 platinum ears from medium sl. 350 (20 ml) is inoculated and added with cellulase (Sumiteam (registered trademark) AC: Shinnippon Chemical Co., Ltd.) 1.0 (w / v)%, which has been sterilized by UV irradiation for 10 minutes, at 30 ° C. The culture was shaken for about 24 hours (preculture 1). From this preculture 1, a new Medium No. 350 ml (100 ml) was inoculated with 5 ml, and cellulase (Sumiteam® AC) 1.0 (w / v)% sterilized by UV irradiation for 10 minutes was added and cultured with shaking at 30 ° C. for 3 days ( Preculture 2).

<Saccharification of apple juice residue and okara>
On the other hand, after adding water 1 to apple juice residue wet weight 1, autoclaving (121 ° C, 20 min) 700 g of equivalent hydrolyzed residue crushed with a mixer (corresponding to 350 g of water), UV irradiation for 10 minutes 1.75 g of cellulase (Sumiteam <(R)> AC) sterilized by the above was added (corresponding to a residue wet weight ratio of 0.5%) and shaken at 40 [deg.] C. The pH was not adjusted and was 4.3 at this time. Further, after adding water 4 to Okara 1 and adjusting 570 g of 4-fold amount of Okara (corresponding to Okara 114 g and water 456 g) crushed with a mixer from pH 7.3 to 4.3, autoclaving (121 ° C., 20 min) Then, 0.57 g of cellulase (Sumiteam <(R)> AC) sterilized by UV irradiation for 10 minutes (corresponding to 0.5% by weight of okara wet weight) was added and shaken at 40 [deg.] C. After confirming the liquefaction after 12 hours, respectively, the enzyme treatment was terminated, and then autoclaved (121 ° C., 5 min) for enzyme deactivation. Thereafter, the apple juice residue and the saccharified solution of okara were centrifuged (15,200 × g, 30 min), and the supernatant was qualitatively filtered. The saccharified solution was obtained by suction filtration using 2. The saccharified liquid collected at this time was 564 g for apple juice residue and 484 g for okara.

<Preparation of BC production medium and BC production>
The apple juice residue thus obtained and the saccharified liquid of okara were mixed at a mixing ratio shown in Table 4, and the pH was adjusted to a range of 6.8 to 6.9, followed by autoclaving (121 ° C., 20 min. ) To prepare a medium. 5 ml of each medium was dispensed into a sterile petri dish, inoculated with 100 μl of the preculture solution 2 and cultured at 30 ° C. for 1 week.

The obtained bacterial cellulose was repeatedly washed with a 2% SDS aqueous solution or a 4% NaOH aqueous solution, filtered through a glass filter, washed with water, dried and weighed, and this was defined as the bacterial cellulose production.
Table 5 shows the average production amount of bacterial cellulose for each test strain and each saccharified liquid mixture ratio. In the table, the unit of production of bacterial cellulose is mg, and the mixing ratio of each saccharified solution is shown as the amount of saccharified solution (ml) in 100 ml of the total amount of saccharified solution (see Table 4 above). FIG. 4 is a graph of Table 5.

  As shown in these figures, in the case of Gluconacetobacter xylinus NBRC16682, the production of bacterial cellulose increases when the mixing ratio of apple juice residue saccharified solution and okara saccharified solution is 3: 1 to 1: 1. The production amount became maximum in the vicinity of 1 to 1: 2, and production was confirmed even in a medium containing 100% Okara saccharified solution. Bacterial cellulose was not produced when the mixing ratio was 5: 1 or less. On the other hand, NBRC13693 strain which has been used conventionally has a low production amount of bacterial cellulose, and was not produced in a medium containing 100% of okara saccharified solution.

  Bacterial cellulose is a material with excellent properties in terms of cellulose purity, fiber width, Young's modulus, liquid crystal forming ability, etc., and its application fields will continue to expand in the food manufacturing, medical and other industries in general. It is considered a thing. According to the present invention, bacterial cellulose can be produced at low cost by effectively utilizing plant-derived or fungal-derived waste such as apple juice residue. This is also an effective use of waste that must be processed at a high cost. In addition, unlike the conventional acid decomposition method, waste liquid treatment is unnecessary because it is an enzyme treatment method, and at the same time the amount of solid waste is reduced, leading to a reduction in environmental burden. Therefore, the invention has high industrial applicability.

It is a basic flowchart which shows an example of the culture medium manufacturing method for bacterial cellulose of this invention. It is a flowchart which shows the bacterial cellulose manufacturing process in an Example. It is a basic flowchart which shows the other example of the culture medium manufacturing method for bacterial cellulose of this invention. It is a flowchart which shows the example of the bacterial cellulose manufacturing process based on the basic flow shown in FIG. It is a graph which shows the bacterial cellulose production amount by the mixing ratio of an apple juice residue saccharified liquid and an okara saccharified liquid in the manufacturing method shown in FIG. It is a graph which shows the amount of bacterial cellulose production for each pH by each test strain It is a graph which shows the bacterial cellulose production amount for every saccharified liquid mixing ratio for every test strain.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... 1st waste 2 ... 2nd waste 10 ... Enzyme saccharified liquid (1st saccharified liquid)
20 ... Enzyme saccharified solution (second saccharified solution)
23 ... Mixture 24 ... Enzyme saccharified solution 100, 200 ... Bacteria cellulose production medium P1, P2, P22 ... Enzyme saccharification treatment process P3 ... Mixing treatment process P4, P23 ... pH adjustment treatment process P21 ... Mixing treatment process

Claims (9)

A medium for producing bacterial cellulose, comprising a vegetable waste that can be enzymatically saccharified and that also serves as a nitrogen source as a raw material. A medium for producing bacterial cellulose, which is a vegetable waste that can be enzymatically saccharified and also serves as a nitrogen source (hereinafter referred to as “first waste”), and another vegetable waste that can be enzymatically saccharified (hereinafter referred to as “ A medium for producing bacterial cellulose using “second waste” as a raw material. The medium for producing bacterial cellulose according to claim 1, wherein the first waste is okara. The culture medium for producing bacterial cellulose according to claim 2, wherein the first waste is Okara and the second waste is a juice residue from an edible part of the plant body. The culture medium for producing bacterial cellulose according to claim 2, wherein the first waste is Okara and the second waste is apple fruit juice residue. An enzyme saccharified solution (hereinafter referred to as “first saccharified solution”) is obtained from the first waste, and an enzyme saccharified solution (hereinafter referred to as “second saccharified solution”) is obtained from the second waste. The medium for producing bacterial cellulose according to any one of claims 2, 4 and 5, wherein the medium is prepared by mixing the first saccharified solution and the second saccharified solution. The medium for producing bacterial cellulose according to claim 6, wherein the first saccharified solution is used in an amount of 25% by weight or more of the second saccharified solution. 6. The bacterial cellulose production medium according to claim 2, wherein an enzymatic saccharified solution is produced from a mixture of the first waste and the second waste. A method for producing bacterial cellulose, comprising culturing cellulose-producing bacteria using the medium for producing bacterial cellulose according to claim 1 to obtain bacterial cellulose.

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