CN106389150A - Preparation method of hyaluronic acid-bacterial cellulose biological mask - Google Patents
Preparation method of hyaluronic acid-bacterial cellulose biological mask Download PDFInfo
- Publication number
- CN106389150A CN106389150A CN201611052457.2A CN201611052457A CN106389150A CN 106389150 A CN106389150 A CN 106389150A CN 201611052457 A CN201611052457 A CN 201611052457A CN 106389150 A CN106389150 A CN 106389150A
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- CN
- China
- Prior art keywords
- hyaluronic acid
- bacterial cellulose
- preparation
- facial film
- culture
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/141—Feedstock
Abstract
The invention discloses a preparation method of a hyaluronic acid-bacterial cellulose biological mask. The preparation method comprises the following steps: inoculating an activated acetobacter gluconicum strain 1.1812 serving as a strain seed into a culture medium, and performing static culture at the constant temperature of 30 DEG C for 4 to 7 days; adding hyaluronic acid, sealing through a silicone mold, and performing static culture at the constant temperature of 30 DEG C for 5 days; taking out a mask obtained by culture, cleaning by ultrapure water, boiling in ultrapure water with the temperature of 100 DEG C for 25 to 60 minutes, soaking the mask in ultrapure water for 30 minutes to obtain a white transparent mask, and performing high-pressure sterilization and plastic packaging to obtain the hyaluronic acid-bacterial cellulose biological mask. The hyaluronic acid-bacterial cellulose biological mask is high in tensile strength and elastic modulus under a wet condition, is not liable to deform after being drawn, can keep a uniform hole structure, is high in skin stickiness, high in skin friendliness, high in water holding capacity and low in water vapor permeation rate, and can effectively prevent evaporation of water and further lock the water.
Description
Technical field
The invention belongs to the preparation field of facial film and in particular to a kind of hyaluronic acid-system of Bacterial cellulose biology facial film
Preparation Method.
Background technology
With the development in epoch, people increasingly pay attention to the maintenance to personal skin quality, and facial film has become a kind of common shield
Skin product.It is apply or spread on human skin, take off after a period of time from, clean or retain, play colonization or cleaning
The product of effect.The basic function of facial film is that skin is hedged off from the outer world, and so that skin temperature is raised, pore is perspired in a large number, skin
Secretory activity vigorous, when peeling off facial film, skin secretion, scurf, dirt etc. are removed, by face while making pore clean
Nutrient substance in film effectively penetrates in skin, plays the effect promoting skin function.Molding class facial film is by face mask substrate material warp
Essence is added to make after folding into bag.Preferably face mask substrate material should possess following performance：Good retentiveness；Excellent patch
Face degree；Good biocompatibility；Good mechanical property；Degradable, pollution-free.At present, existing face mask substrate material is in moistening
Limited strength under state, and paste that skin degree is not good, the using effect of impact facial film.
Content of the invention
The present invention provides a kind of preparation method of hyaluronic acid-Bacterial cellulose biology facial film.
The present invention is achieved by the following technical solutions.
The preparation method of hyaluronic acid-Bacterial cellulose biology facial film, comprises the following steps：
（1）The preparation of bacterial cellulose facial mask base material：Acetobacter gluconicum bacterial strain 1.1812 will be activated as strain, according to volume ratio
1:100 are seeded in culture medium, and the composition of wherein said culture medium is：Glucose 5%, peptone 0.5%, yeast extract 0.5%,
Citric acid 0.1%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%, remaining be water；Postvaccinal culture medium is poured into sterilized
Facial film mould in, with pellosil sealing, 30 DEG C of constant temperature quiescent culture 4-7 days；
（2）The preparation of hyaluronic acid-Bacterial cellulose biology facial film：According to mass ratio 1:Hyaluronic acid is added step by 100（1）
In described product, Silicon moulds seal, 30 DEG C of constant temperature quiescent culture 5 days；
（3）The facial film that obtains of culture is taken out, ultra-pure water cleans, and boils 25-60min in 100 DEG C of ultra-pure water, then with ultrapure
Water soaks 30min, obtains white clear shape diaphragm, autoclaving, plastic sealing pack, prepared hyaluronic acid-Bacterial cellulose biosurface
Film.
Preferably, step（1）In, 30 DEG C of constant temperature quiescent culture 5 days.
Preferably, step（3）In, boil 45min in 100 DEG C of ultra-pure water.
Advantages of the present invention：
The facial film of present invention preparation is high in hygrometric state condition Tensile strength and elastic modelling quantity, pulls and is unlikely to deform and maintains uniformly
Pore space structure, patch skin degree is high, and skin-friendly is good, and water retention property is good, and moisture-vapor transmission is low, effectively can prevent moisture evaporation, enter
And pin moisture.
Specific embodiment
Embodiment 1
The preparation method of hyaluronic acid-Bacterial cellulose biology facial film, comprises the following steps：
（1）The preparation of bacterial cellulose facial mask base material：Acetobacter gluconicum bacterial strain 1.1812 will be activated as strain, according to volume ratio
1:100 are seeded in culture medium, and the composition of wherein said culture medium is：Glucose 5%, peptone 0.5%, yeast extract 0.5%,
Citric acid 0.1%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%, remaining be water；Postvaccinal culture medium is poured into sterilized
Facial film mould in, with pellosil sealing, 30 DEG C of constant temperature quiescent culture 4 days；
（2）The preparation of hyaluronic acid-Bacterial cellulose biology facial film：According to mass ratio 1:Hyaluronic acid is added step by 100（1）
In described product, Silicon moulds seal, 30 DEG C of constant temperature quiescent culture 5 days；
（3）The facial film that culture is obtained takes out, and ultra-pure water cleans, and boils 25min, then use ultra-pure water in 100 DEG C of ultra-pure water
Soak 30min, obtain white clear shape diaphragm, autoclaving, plastic sealing pack, prepared hyaluronic acid-Bacterial cellulose biology facial film.
Embodiment 2
The preparation method of hyaluronic acid-Bacterial cellulose biology facial film it is characterised in that：Comprise the following steps：
（1）The preparation of bacterial cellulose facial mask base material：Acetobacter gluconicum bacterial strain 1.1812 will be activated as strain, according to volume ratio
1:100 are seeded in culture medium, and the composition of wherein said culture medium is：Glucose 5%, peptone 0.5%, yeast extract 0.5%,
Citric acid 0.1%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%, remaining be water；Postvaccinal culture medium is poured into sterilized
Facial film mould in, with pellosil sealing, 30 DEG C of constant temperature quiescent culture 5 days；
（2）The preparation of hyaluronic acid-Bacterial cellulose biology facial film：According to mass ratio 1:Hyaluronic acid is added step by 100（1）
In described product, Silicon moulds seal, 30 DEG C of constant temperature quiescent culture 5 days；
（3）The facial film that culture is obtained takes out, and ultra-pure water cleans, and boils 45min, then use ultra-pure water in 100 DEG C of ultra-pure water
Soak 30min, obtain white clear shape diaphragm, autoclaving, plastic sealing pack, prepared hyaluronic acid-Bacterial cellulose biology facial film.
Embodiment 3
The preparation method of hyaluronic acid-Bacterial cellulose biology facial film it is characterised in that：Comprise the following steps：
（1）The preparation of bacterial cellulose facial mask base material：Acetobacter gluconicum bacterial strain 1.1812 will be activated as strain, according to volume ratio
1:100 are seeded in culture medium, and the composition of wherein said culture medium is：Glucose 5%, peptone 0.5%, yeast extract 0.5%,
Citric acid 0.1%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%, remaining be water；Postvaccinal culture medium is poured into sterilized
Facial film mould in, with pellosil sealing, 30 DEG C of constant temperature quiescent culture 7 days；
（2）The preparation of hyaluronic acid-Bacterial cellulose biology facial film：According to mass ratio 1:Hyaluronic acid is added step by 100（1）
In described product, Silicon moulds seal, 30 DEG C of constant temperature quiescent culture 5 days；
（3）The facial film that culture is obtained takes out, and ultra-pure water cleans, and boils 60min, then use ultra-pure water in 100 DEG C of ultra-pure water
Soak 30min, obtain white clear shape diaphragm, autoclaving, plastic sealing pack, prepared hyaluronic acid-Bacterial cellulose biology facial film.
Claims (3)
1. hyaluronic acid-Bacterial cellulose biology facial film preparation method it is characterised in that：Comprise the following steps：
（1）The preparation of bacterial cellulose facial mask base material：Acetobacter gluconicum bacterial strain 1.1812 will be activated as strain, according to volume ratio
1:100 are seeded in culture medium, and the composition of wherein said culture medium is：Glucose 5%, peptone 0.5%, yeast extract 0.5%,
Citric acid 0.1%, disodium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.1%, remaining be water；Postvaccinal culture medium is poured into sterilized
Facial film mould in, with pellosil sealing, 30 DEG C of constant temperature quiescent culture 4-7 days；
（2）The preparation of hyaluronic acid-Bacterial cellulose biology facial film：According to mass ratio 1:Hyaluronic acid is added step by 100（1）
In described product, Silicon moulds seal, 30 DEG C of constant temperature quiescent culture 5 days；
（3）The facial film that obtains of culture is taken out, ultra-pure water cleans, and boils 25-60min in 100 DEG C of ultra-pure water, then with ultrapure
Water soaks 30min, obtains white clear shape diaphragm, autoclaving, plastic sealing pack, prepared hyaluronic acid-Bacterial cellulose biosurface
Film.
2. hyaluronic acid according to claim 1-Bacterial cellulose biology facial film preparation method it is characterised in that：Step
Suddenly（1）In, 30 DEG C of constant temperature quiescent culture 5 days.
3. hyaluronic acid according to claim 1-Bacterial cellulose biology facial film preparation method it is characterised in that：Step
Suddenly（3）In, boil 45min in 100 DEG C of ultra-pure water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611052457.2A CN106389150A (en) | 2016-11-25 | 2016-11-25 | Preparation method of hyaluronic acid-bacterial cellulose biological mask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611052457.2A CN106389150A (en) | 2016-11-25 | 2016-11-25 | Preparation method of hyaluronic acid-bacterial cellulose biological mask |
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| Publication Number | Publication Date |
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| CN106389150A true CN106389150A (en) | 2017-02-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611052457.2A Pending CN106389150A (en) | 2016-11-25 | 2016-11-25 | Preparation method of hyaluronic acid-bacterial cellulose biological mask |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860030A (en) * | 2017-03-08 | 2017-06-20 | 张仕英 | A kind of preparation of ultra micro nano-pore structure biology cellulose facial mask |
| CN107049800A (en) * | 2017-03-08 | 2017-08-18 | 张仕英 | A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid |
| CN107157780A (en) * | 2017-05-19 | 2017-09-15 | 陕西农产品加工技术研究院 | A kind of preparation method of aloe bacteria cellulose Pure natural mask |
| CN108451791A (en) * | 2018-07-02 | 2018-08-28 | 河南工业大学 | A kind of preparation method of sericin/hyaluronic acid-bacteria cellulose compound bio facial mask |
| CN108685722A (en) * | 2018-07-06 | 2018-10-23 | 佛山皖阳生物科技有限公司 | A kind of preparation method of face mask substrate material |
| CN109021296A (en) * | 2017-06-08 | 2018-12-18 | 南京理工大学 | A kind of bamboo charcoal/biology cellulose composite material and preparation method and application |
| CN110184741A (en) * | 2019-05-10 | 2019-08-30 | 上海棉芙生物科技有限公司 | A kind of banana fiber mask substrate and preparation method thereof |
| CN111686206A (en) * | 2020-06-02 | 2020-09-22 | 北京致成生物医学科技有限公司 | Moisturizing and repairing bacterial cellulose mask and preparation method thereof |
-
2016
- 2016-11-25 CN CN201611052457.2A patent/CN106389150A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860030A (en) * | 2017-03-08 | 2017-06-20 | 张仕英 | A kind of preparation of ultra micro nano-pore structure biology cellulose facial mask |
| CN107049800A (en) * | 2017-03-08 | 2017-08-18 | 张仕英 | A kind of preparation method of the nano-cellulose work(facial mask containing plant extraction liquid |
| CN107157780A (en) * | 2017-05-19 | 2017-09-15 | 陕西农产品加工技术研究院 | A kind of preparation method of aloe bacteria cellulose Pure natural mask |
| CN109021296A (en) * | 2017-06-08 | 2018-12-18 | 南京理工大学 | A kind of bamboo charcoal/biology cellulose composite material and preparation method and application |
| CN108451791A (en) * | 2018-07-02 | 2018-08-28 | 河南工业大学 | A kind of preparation method of sericin/hyaluronic acid-bacteria cellulose compound bio facial mask |
| CN108685722A (en) * | 2018-07-06 | 2018-10-23 | 佛山皖阳生物科技有限公司 | A kind of preparation method of face mask substrate material |
| CN110184741A (en) * | 2019-05-10 | 2019-08-30 | 上海棉芙生物科技有限公司 | A kind of banana fiber mask substrate and preparation method thereof |
| CN111686206A (en) * | 2020-06-02 | 2020-09-22 | 北京致成生物医学科技有限公司 | Moisturizing and repairing bacterial cellulose mask and preparation method thereof |
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| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170215 |
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| WD01 | Invention patent application deemed withdrawn after publication |