CN111686045A - Skin-tendering facial mask made of plant-derived bacterial cellulose and preparation method of facial mask - Google Patents
Skin-tendering facial mask made of plant-derived bacterial cellulose and preparation method of facial mask Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses a plant-derived bacterial cellulose skin-tendering facial mask which comprises the following components in parts by weight: 60-90 parts of bacterial cellulose and 10-20 parts of active parts of natural plants. According to the invention, natural plant active ingredients are organically combined with bacterial cellulose, the bacterial cellulose facial mask with any shape and shape is naturally cultured by utilizing the natural growth of the bacterial cellulose membrane to form any shape, the shape is not required to be processed in a later stage, and the skin-tendering anti-aging skin-care hydrogel facial mask matrix containing various natural extracts and bioactive polypeptides is prepared, so that the facial mask is more conformable and close to the skin. Meanwhile, the nutrient components in the mask are slowly released and effectively absorbed by the skin, allergy is reduced, and multiple functions of skin tendering, moisture retention and moisturizing, oxidation resistance, skin repair and the like are combined, so that the skin nutrition metabolism is improved, the skin elasticity is kept, and meanwhile, the epidermal tissue is repaired, and therefore multiple excellent effects of skin tendering, skin care and aging resistance are achieved.
Description
Technical Field
The invention relates to the technical field of biomedicine, and particularly relates to a skin tendering facial mask made of plant-derived bacterial cellulose and a preparation method thereof.
Background
Skin aging is a complex series of biological processes within the skin tissue, and is the result of the combined action of all endogenous physiological factors and exogenous environmental factors. Furthermore, genetic factors and the influence of expression levels of relevant aging genes and proteins, as well as long-term exposure to large doses of sunlight and ultraviolet rays, are important factors in causing skin aging. Skin aging not only affects normal skin tissue structure and physiological function, but also directly affects the appearance and appearance of the outer layer of the skin. Therefore, anti-aging and anti-oxidation are highly regarded by beauty-seeking people.
The facial mask is a skin care product for facial beautification, and is used as a carrier for facial cleaning, nursing and nutrition components, and is applied on the face to realize beautifying functions, such as moisturizing, whitening, anti-aging, grease balancing and the like. Typical masks include several types: a paste type, a tear type, a jelly type, and a wet tissue type. The materials of the common facial mask mainly comprise blended fabric, all cotton, silk and terylene. In a common facial mask, cotton cloth and blended fabric are often soaked in a solution containing skin care essence to obtain the facial mask with the adsorbed essence. However, the mask has the defects that the water content is general, the water is easy to evaporate, and the mask absorbs the water on the face due to the fact that the mask is attached on the face for a long time. A plurality of hydrogel masks with high price appear on the market at present, the hydrogel masks have high water holding rate, can adsorb a large amount of nutrient substances and water, have good skin-friendly property, and can be attached to the face for a long time without sucking the moisture of the skin backwards.
Bacterial Cellulose (BC) is a Cellulose of Bacterial size secreted by acetobacter xylinum during growth movements. A large number of researches prove that the BC has good biocompatibility and no short-term or long-term toxicity to various cells, in addition, abundant hydroxyl groups on the BC structure can provide a large number of active functional groups for chemical modification of the BC, and a fine three-dimensional network structure formed by mutually interweaving fibers enables the BC to have good mechanical properties, so that the BC is widely researched and applied in the aspects of biomedicine, such as wound repair, artificial blood vessels, drug sustained-release carriers, artificial intervertebral discs, neural interface research and the like.
In view of the above, how to find a skin-tendering and brightening mask and a carrier thereof is a problem which needs to be solved urgently by those skilled in the art.
Disclosure of Invention
The invention aims to provide a skin-tendering facial mask solution of plant-derived bacterial cellulose and a preparation method thereof.
In order to realize the purpose of the invention, the invention firstly provides a plant-derived bacterial cellulose skin-tendering facial mask which comprises the following components in parts by weight: 60-90 parts of bacterial cellulose and 10-20 parts of active parts of natural plants.
Preferably, the skin rejuvenation mask further comprises other components, wherein the other components account for 10-25 parts by weight, and the other components comprise polypeptide, humectant, chelating agent, dispersant, emulsifier, thickener, preservative, buffer and/or solvent.
The polypeptide is: collagen, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7; the humectant is as follows: polyglutamic acid, sodium hyaluronate and tetrahydro methyl pyrimidine carboxylic acid; the chelating agent is disodium EDTA, the dispersing agent is glycerol, the emulsifying agent is polysorbate-20, the thickening agent is Chondrus crispus (Chondrus crispus) powder, carob bean (Ceratoniasiliqua) gum, hydroxyethyl cellulose and carbomer, the preservative is methylparaben, phenoxyethanol and chlorphenesin, the buffering agent is potassium chloride, and the solvent is deionized water, propylene glycol and butanediol.
Preferably, the natural plant active part is one or more of grape seeds or extracts thereof, centella asiatica or extracts thereof, haematococcus pluvialis or extracts thereof, and saussurea involucrate or extracts thereof.
The components in the active part of the natural plant and the extract thereof are all products sold in the market, and the haematococcus pluvialis, the snow lotus, the grape seeds and the snow grass are preferably selected.
Haematococcus pluvialis: haematococcus pluvialis belongs to the order Volvocales, the family Haematococcus. Haematococcus pluvialis is recognized as the best organism in nature to produce natural astaxanthin. Haematococcus pluvialis contains 1.5% to 3.0% astaxanthin and is considered to be a "concentrate" of natural astaxanthin. One of the strongest natural antioxidants of natural astaxanthin in the world effectively eliminates oxygen free radicals in cells, enhances the regeneration capability of the cells, maintains the balance of the organism and reduces the accumulation of aged cells, and protects the health of the cells and DNA from inside to outside, thereby protecting the health of skin. The haematococcus pluvialis extract can permeate into each skin cell, rebuild collagen and protein matrix, and rebuild skin elasticity; firming the skin 'heat preservation barrier' and fully keeping the skin moisture, thereby playing the roles of moisturizing and anti-wrinkle.
Snow lotus grows in rock seams, stone walls and gravel beaches near the altitude of 2400 + 4000 meters high mountain snow lines. The environment for the growth of the snow lotus peanut is extremely harsh, general plants in the growing environment cannot survive at all, and the snow lotus can grow in a severe cold environment with minus dozens of degrees and in an oxygen-deficient environment with thin air in a blowing-in and blowing-out manner and be hard to grow. It is the saussurea involucrate which has unique living habit and unique growing environment, makes it natural and rare, and brings about its unique pharmacological action and medicinal value. The snow lotus herb is applied in the skin care product to accelerate the metabolism of the skin, reduce wrinkles, keep the skin glossy and plump and delay aging.
Polyphenols extracted and separated from grape seeds are one of the most effective antioxidants from plant sources discovered so far, and mainly comprise polyphenols such as procyanidine, catechin, epicatechin, gallic acid, epicatechin gallate and the like. Wherein, the procyanidine is a novel high-efficiency antioxidant, is the most effective free radical scavenger discovered so far, has very strong in vivo activity and is quickly and completely absorbed; the procyanidine can also reduce the risk of heart disease, cancer, senilism, arthritis and the like, and has the effects of enhancing the elasticity and smoothness of skin, preventing radiation damage of sunlight to the skin and the like.
The asiatic centella is rich in asiaticoside and madecassoside, wherein the asiaticoside has the function of promoting the activity of scar fibroblasts, so that the asiaticoside has the function of removing scars, has a good polymerization effect with phospholipid substances, and has a remarkable effect of removing whelks. The centella asiatica extract can promote the formation of collagen in the dermis, has the effects of making the skin compact and soft, and can promote blood circulation, improve the acne skin and prevent pigmentation.
Further, the invention also provides a preparation method of the bacterial cellulose skin-tendering mask, which comprises the following steps:
(1) preparation of plant extract
Cleaning and naturally airing active parts of natural plants, crushing the active parts of the natural plants into raw material powder with the average particle size of more than 100 meshes, mixing 50% ethanol serving as a leaching solution with plant powder according to the mass ratio of 1: 1-10, carrying out ultrasonic treatment for 20-30 min, transferring the mixture into a leaching tank, leaching for 1-2 h at the temperature of 50-100 ℃, filtering, and carrying out reduced pressure evaporation on the filtrate to separate the solution of the extracting solution to obtain the plant extracting solution for the facial mask;
(2) preparation of bacterial cellulose
Inoculating acetobacter xylinum gluconicum into a culture medium, culturing for 3-5 days at 25-30 ℃, collecting bacterial cellulose, soaking in deionized water for 2 days, boiling with 0.1mol/L NaCl solution for 40min, and then soaking in deionized water and cleaning for later use;
(3) preparation of facial mask
And (3) sterilizing the bacterial cellulose obtained in the step (2) at high temperature and high pressure, soaking the bacterial cellulose in the plant extracting solution obtained in the step (1), and simultaneously adding polypeptide, a humectant, a chelating agent, a dispersing agent, an emulsifier, a thickening agent, a preservative, a buffering agent and a solvent for 20-30 hours to obtain the plant-derived bacterial cellulose rejuvenation sheet-shaped mask.
In some embodiments of the above mask of the present invention, the bacterial cellulose is obtained by culturing acetobacter xylinum xylose. Further, the acetobacter xylinum is acetobacter xylinum ATCC 53582.
In some embodiments of the above mask of the present invention, the bacterial cellulose is aqueous bacterial cellulose.
Advantageous effects
According to the invention, natural plant active ingredients are organically combined with bacterial cellulose, and the bacterial cellulose facial mask with any shape and shape is naturally cultured by utilizing the natural growth of the bacterial cellulose membrane to form any shape, so that the skin-tendering anti-aging skin-care hydrogel facial mask matrix containing various natural extracts and bioactive polypeptides is prepared without post-processing shape, and the facial mask is more conformable and close to the skin; the facial mask has good mechanical properties and high water holding property, and can not suck water on skin and cause skin injury after being applied to face for a long time. Meanwhile, the nutrient components in the mask are slowly released and effectively absorbed by the skin, allergy is reduced, and multiple functions of skin tendering, moisture retention and moisturizing, oxidation resistance, skin repair and the like are combined, so that the skin nutrition metabolism is improved, the skin elasticity is kept, and meanwhile, the epidermal tissue is repaired, and therefore multiple excellent effects of skin tendering, skin care and aging resistance are achieved.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1: haematococcus pluvialis mask
The components are as follows: 60 parts of bacterial cellulose and 12 parts of haematococcus pluvialis active part; 2 parts of collagen, 2 parts of palmitoyl tripeptide-1 and 1 part of palmitoyl tetrapeptide-7; 2 parts of dipotassium glycyrrhizinate and 0.01 part of allantoin; 1 part of polyglutamic acid, 0.01 part of sodium hyaluronate and 1 part of tetrahydro-methyl pyrimidine carboxylic acid; 0.01 part of EDTA disodium, 1 part of glycerol, 0.01 part of polysorbate-20, 0.1 part of carrageen crispus powder, 5 parts of carob bean gum, 1 part of hydroxyethyl cellulose, 0.1 part of carbomer, 0.05 part of methylparaben, 0.5 part of phenoxyethanol, 0.1 part of chlorphenesin, 0.1 part of potassium chloride, 0.5 part of propylene glycol and 0.3 part of butanediol.
The preparation method comprises the following specific steps:
(1) preparation of plant extract
Cleaning and naturally airing an active part of haematococcus pluvialis, crushing the haematococcus pluvialis into raw material powder with the average particle size of more than 100 meshes, mixing 50% of ethanol serving as a leaching solution and plant powder according to the mass ratio of 1: 1-10, carrying out ultrasonic treatment for 20-30 min, transferring the mixture into a leaching tank, leaching for 1-2 h at the temperature of 50-100 ℃, filtering, and carrying out reduced pressure evaporation on the filtrate to separate the solution of the extracting solution to obtain the plant extracting solution for the mask;
(2) preparation of bacterial cellulose
Inoculating acetobacter xylinum gluconicum into a culture medium, culturing for 3-5 days at 25-30 ℃, collecting bacterial cellulose, soaking in deionized water for 2 days, boiling with 0.1mol/L NaCl solution for 40min, and then soaking in deionized water and cleaning for later use;
(3) preparation of facial mask
And (3) sterilizing the bacterial cellulose obtained in the step (2) at high temperature and high pressure, soaking the bacterial cellulose in the plant extracting solution obtained in the step (1), and simultaneously adding polypeptide, a humectant, a chelating agent, a dispersing agent, an emulsifier, a thickening agent, a preservative, a buffering agent and a solvent for 20-30 hours to obtain the plant-derived bacterial cellulose rejuvenation sheet-shaped mask.
Example 2: saussurea involucrate face pack
The components are as follows: 80 parts of bacterial cellulose and 18 parts of saussurea involucrate active part; 1 part of collagen, 1 part of palmitoyl tripeptide-1 and 0.01 part of palmitoyl tetrapeptide-7; 1 part of dipotassium glycyrrhizinate and 1 part of allantoin; 0.5 part of polyglutamic acid, 0.01 part of sodium hyaluronate and 0.5 part of tetrahydro-methylpyrimidine carboxylic acid; 0.5 part of EDTA disodium, 2 parts of glycerol, 0.5 part of polysorbate-20, 1 part of carrageen crispus powder, 4 parts of carob bean gum, 2 parts of hydroxyethyl cellulose, 0.5 part of carbomer, 0.1 part of methyl hydroxybenzoate, 0.3 part of phenoxyethanol, 0.2 part of chlorphenesin, 1 part of potassium chloride, 1 part of propylene glycol and 1 part of butanediol, and the balance of deionized water.
The preparation method comprises the following specific steps:
(1) cleaning and naturally airing the active part of the saussurea involucrate, crushing the active part of the saussurea involucrate into raw material powder with the average particle size of more than 100 meshes, mixing 50% ethanol serving as a leaching solution with plant powder according to the mass ratio of 1: 1-10, carrying out ultrasonic treatment for 20-30 min, transferring the mixture into a leaching tank, leaching for 1-2 h at the temperature of 50-100 ℃, filtering, and carrying out reduced pressure evaporation on the filtrate to separate the solution after the extracting solution to obtain the plant extracting solution for the facial mask;
(2) preparation of bacterial cellulose
Inoculating acetobacter xylinum gluconicum into a culture medium, culturing for 3-5 days at 25-30 ℃, collecting bacterial cellulose, soaking in deionized water for 2 days, boiling with 0.1mol/L NaCl solution for 40min, and then soaking in deionized water and cleaning for later use;
(3) preparation of facial mask
And (3) sterilizing the bacterial cellulose obtained in the step (2) at high temperature and high pressure, soaking the bacterial cellulose in the plant extracting solution obtained in the step (1), and simultaneously adding polypeptide, a humectant, a chelating agent, a dispersing agent, an emulsifier, a thickening agent, a preservative, a buffering agent and a solvent for 20-30 hours to obtain the plant-derived bacterial cellulose rejuvenation sheet-shaped mask.
Example 3: grape seed mask
The components are as follows: 90 parts of bacterial cellulose and 15 parts of grape seed active part; 0.5 parts collagen, 2 parts palmitoyl tripeptide-1 and 1 part palmitoyl tetrapeptide-7; 1 part of dipotassium glycyrrhizinate and 1 part of allantoin; 1 part of polyglutamic acid, 1 part of sodium hyaluronate and 1 part of tetrahydro-methyl pyrimidine carboxylic acid; 1 part of EDTA disodium, 4 parts of glycerol, 1 part of polysorbate-20, 4 parts of carrageen crispus powder, 1 part of carob bean gum, 0.5 part of hydroxyethyl cellulose, 1 part of carbomer, 0.1 part of methylparaben, 0.5 part of phenoxyethanol, 0.1 part of chlorphenesin, 0.5 part of potassium chloride, 1 part of propylene glycol and 5 parts of butanediol, and the balance of deionized water.
The preparation method comprises the following specific steps:
(1) cleaning and naturally airing an active part of grape seeds, crushing the active part into raw material powder with the average particle size of more than 100 meshes, mixing 50% ethanol serving as a leaching solution with plant powder according to the mass ratio of 1: 1-10, carrying out ultrasonic treatment for 20-30 min, transferring the mixture into a leaching tank, leaching for 1-2 h at the temperature of 50-100 ℃, filtering, and carrying out reduced pressure evaporation on the filtrate to separate the solution after the extracting solution to obtain the plant extracting solution for the facial mask;
(2) preparation of bacterial cellulose
Inoculating acetobacter xylinum gluconicum into a culture medium, culturing for 3-5 days at 25-30 ℃, collecting bacterial cellulose, soaking in deionized water for 2 days, boiling with 0.1mol/L NaCl solution for 40min, and then soaking in deionized water and cleaning for later use;
(3) preparation of facial mask
And (3) sterilizing the bacterial cellulose obtained in the step (2) at high temperature and high pressure, soaking the bacterial cellulose in the plant extracting solution obtained in the step (1), and simultaneously adding polypeptide, a humectant, a chelating agent, a dispersing agent, an emulsifier, a thickening agent, a preservative, a buffering agent and a solvent for 20-30 hours to obtain the plant-derived bacterial cellulose rejuvenation sheet-shaped mask.
Example 4: biocompatibility experiment of cellulose mask leaching liquor
Considering the mask attached to the epidermis of the face, we selected human immortalized keratinocytes HaCAT cells as experimental cells. Mixing the three plant source bacterial cellulose facial masks with a cell culture medium according to a ratio of 0.1g/mL, leaching at 37 ℃ for 24h, centrifuging at 10000rpm for 5min, taking supernatant to obtain leaching liquor, and taking the leaching liquor to perform a cell biocompatibility experiment. Inoculating cells on a 96-well plate, 10000/well, changing a cell culture solution into a leaching solution after 24 hours, and measuring the cell activity (n is more than or equal to 4) for 1, 3 and 5 days by using CCK-8 to test results. The results show that in the culture process of 5 days, compared with the positive result (PS), the three cellulose facial mask leaching solutions have good biocompatibility, which indicates that the facial mask has no toxic or side effect.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. The skin tendering facial mask is characterized by comprising the following components in parts by weight: 60-90 parts of bacterial cellulose and 10-20 parts of active parts of natural plants.
2. The skin rejuvenation mask as set forth in claim 1 further including from 10 to 25 parts by weight of other components including polypeptides, humectants, chelating agents, dispersing agents, emulsifiers, thickeners, preservatives, buffers and/or solvents.
3. The skin rejuvenation mask as claimed in claim 1 or 2, wherein said natural plant active fraction is one or more of grape seeds or extracts thereof, centella asiatica or extracts thereof, haematococcus pluvialis or extracts thereof, saussurea involucrate or extracts thereof.
4. A preparation method of a skin-tendering facial mask of bacterial cellulose is characterized in that,
(1) preparation of plant extract
Cleaning and naturally airing active parts of natural plants, crushing the active parts of the natural plants into raw material powder with the average particle size of more than 100 meshes, mixing 50% ethanol serving as a leaching solution with plant powder according to the mass ratio of 1: 1-10, carrying out ultrasonic treatment for 20-30 min, transferring the mixture into a leaching tank, leaching for 1-2 h at the temperature of 50-100 ℃, filtering, and carrying out reduced pressure evaporation on the filtrate to separate the solution of the extracting solution to obtain the plant extracting solution for the facial mask;
(2) preparation of bacterial cellulose
Inoculating acetobacter xylinum gluconicum into a culture medium, culturing for 3-5 days at 25-30 ℃, collecting bacterial cellulose, soaking in deionized water for 2 days, boiling with 0.1mol/L NaCl solution for 40min, and then soaking in deionized water and cleaning for later use;
(3) preparation of facial mask
And (3) sterilizing the bacterial cellulose obtained in the step (2) at high temperature and high pressure, soaking the bacterial cellulose in the plant extracting solution obtained in the step (1), and simultaneously adding polypeptide, a humectant, a chelating agent, a dispersing agent, an emulsifier, a thickening agent, a preservative, a buffering agent and a solvent for 20-30 hours to obtain the plant-derived bacterial cellulose rejuvenation sheet-shaped mask.
5. The method for preparing a skin rejuvenation mask as claimed in claim 4, wherein said bacterial cellulose is obtained by culturing Acetobacter xylinum xylose.
6. The method for preparing a skin rejuvenation mask as claimed in claim 5, wherein said acetobacter xylinum is acetobacter xylinum ATCC 53582.
7. The method of preparing a skin rejuvenation mask as claimed in claim 4 wherein said bacterial cellulose is aqueous bacterial cellulose.
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CN112675096A (en) * | 2021-01-22 | 2021-04-20 | 上海加新生物科技有限公司 | Bacteriostatic and bactericidal plant composite composition and preparation method thereof |
CN113712835A (en) * | 2021-09-30 | 2021-11-30 | 前研化妆品科技(上海)有限公司 | Cleansing oil containing astaxanthin liposome and preparation method thereof |
CN115998636A (en) * | 2023-03-24 | 2023-04-25 | 江苏亨瑞生物医药科技有限公司 | Gel mask containing recombinant humanized collagen and preparation method thereof |
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