CN113648259A

CN113648259A - Toning lotion composition containing skin-firming liposome and preparation method thereof

Info

Publication number: CN113648259A
Application number: CN202111126537.9A
Authority: CN
Inventors: 周紫燕; 杨帆; 祝希宇; 郭苗; 周正; 郭阳
Original assignee: Hubei Maishite Biotechnology Co Ltd
Current assignee: Hubei Maishite Biotechnology Co Ltd
Priority date: 2021-09-26
Filing date: 2021-09-26
Publication date: 2021-11-16

Abstract

The invention discloses a toning lotion composition containing liposome with skin tightening effect and a preparation method thereof. The toning lotion composition comprises the following components in percentage by weight: 0.05-35% of skin-tightening efficacy composition liposome, 5-30% of humectant, 0-30% of skin conditioner, 0-10% of thickening agent, 0.01-2% of chelating agent, 0.01-1% of solubilizer, a proper amount of pH regulator, a proper amount of preservative, 0.01-1.0% of daily essence and the balance of deionized water. Wherein the effective composition comprises Ascophyllum nodosum extract, mevalonolactone and Criste anisetree callus culture filtrate. The invention prepares the three functional components into the functional composition liposome which is added into cosmetics, can promote transdermal absorption, effectively acts on two skin layers of skin epidermis and dermis, enhances the skin elasticity and tightens the skin. The skin firming lotion disclosed by the invention achieves a safe and effective skin firming effect by scientifically combining the raw materials and utilizing the synergistic effect among the functional components, so that the skin is full of elasticity, and the young skin state is kept.

Description

Toning lotion composition containing skin-firming liposome and preparation method thereof

Technical Field

The invention relates to a skin care product and a preparation method thereof, belongs to the technical field of daily chemicals, and particularly relates to a toning lotion composition containing skin-tightening liposome and a preparation method thereof.

Background

In daily life, skin sagging is a very disturbing skin problem that is difficult to cover simply by means of make-up or the like. Generally, as a person ages, the loss of collagen and lipids causes the skin to age and relax. In addition, the microbial balance of the skin is disrupted, the skin is exposed to a polluted environment, and factors such as sunlight irradiation and gravity effect also cause aging and relaxation of the skin, and sagging of the skin occurs. Collagen produced by fibroblasts in the dermis is a main component of the dermis and has important functions of maintaining the structure of the skin and endowing the skin with tightness and elasticity, and the content and distribution of the collagen determine whether the skin is young or not.

In the prior art, an anti-aging product mixed with various components such as hydrolyzed collagen, hyaluronic acid, polypeptide, retinol and derivatives thereof is provided, but the anti-aging product is difficult to be absorbed through skin and acts on the dermis of skin to supplement collagen, so that the anti-aging product has an effective tightening effect.

Therefore, how to develop a safe and effective cosmetic with skin-tightening effect becomes a difficult point to be solved by the technicians in the cosmetic industry at present.

Disclosure of Invention

The present invention has an object to provide a toning lotion composition containing liposomes having skin firming effects, which can effectively protect the skin from damage caused by ultraviolet rays, help prevent photoaging of the epidermis and dermis, improve or restore the function of the dermis, stimulate the synthesis of fibers (such as collagen, elastin, and proteoglycan) in the extracellular matrix, and keep the skin moisturized, elastic and firm.

The invention also aims to provide a preparation method of the toning lotion composition with the skin firming effect liposome.

In order to achieve the purpose, the invention adopts the following technical scheme:

the toning lotion composition containing the skin-firming effect liposome comprises the following raw material components in percentage by weight of the total weight of the composition:

0.05-35% firming skin effect composition liposomes, said firming skin effect composition liposomes comprising: ascophyllum nodosum extract 0.01-30%, methyl-tile-acid lactone 0.01-30% and hypecol callus culture filtrate 0.01-30%;

5-30% of humectant;

0-30% of a skin conditioning agent;

0-10% of a thickening agent;

0.3-5.0% of preservative;

0.01-2% of a chelating agent;

0.01-1% of a solubilizer;

0.01-1.0% of daily essence;

proper amount of pH regulator and deionized water.

Further, the cosmetic water composition containing the skin-tightening efficacy liposome comprises the following components in percentage by weight of the total weight of the composition:

0.2-25% firming skin efficacy composition liposomes comprising: 0.05-20% of Ascophyllum nodosum extract, 0.05-20% of methyl-tile lactone and 0.05-20% of hypsizygus marmoreus callus culture filtrate;

further, the firming skin effect composition liposome comprises the following raw material components:

0.05-10% of Ascophyllum nodosum extract, 0.05-10% of methyl-tile lactone and 0.05-10% of hypsizygus marmoreus callus culture filtrate.

0.05-3% of Ascophyllum nodosum extract, 0.05-3% of methyl-tile lactone and 0.05-3% of hypsizygus marmoreus callus culture filtrate.

0.25-10% firming skin efficacy composition liposomes comprising: 0.05-0.5% of Ascophyllum nodosum extract, 0.05-0.5% of methyl-tile lactone and 0.05-0.5% of hypsizygus marmoreus callus culture filtrate;

further, the humectant is selected from one or more of glycerin, propylene glycol, 1, 3-propylene glycol, glyceryl acrylate/acrylic acid copolymer, glyceryl polyether-26, hexylene glycol, 1, 2-pentanediol, caprylyl glycol, ethylhexyl glycerin, polyethylene glycol-32, urea, allantoin, PEG/PPG-17/6 copolymer, PPG-28-Butaneth-35, bis-PEG-18 methyl ether dimethylsilane, sorbitol, and combinations thereof;

the skin conditioner is selected from one or more of honey extract, arginine, lysine, ornithine, sodium lactate, nicotinamide, yeast extract, Hamamelis virginiana extract, rice chaff extract, ammonium glycyrrhizinate, zinc gluconate, caffeine, and biotin;

the thickening agent is selected from one or a combination of acryloyl dimethyl ammonium taurate/VP copolymer, acryloyl dimethyl ammonium taurate/behenyl alcohol polyether-25 methacrylate cross-linked polymer, sodium polyacrylate, acrylic acid (ester) copolymer sodium, polyacrylate-13, hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer, xanthan gum, hydroxyethyl acrylate/acryloyl dimethyl sodium taurate copolymer, carbomer, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer;

the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate and phenoxyethanol;

the chelating agent is one or more selected from EDTA-disodium, EDTA-tetrasodium and the composition thereof;

the solubilizer is one or more selected from PEG-60 hydrogenated castor oil, polysorbate-20 and polysorbate-60.

The pH regulator is selected from one or more of citric acid, sodium citrate, hydroxyethyl piperazine ethane sulfonic acid and a composition thereof.

The preparation process of the composition liposome with the skin tightening effect comprises the following steps:

1) dissolving lecithin and cholesterol in diethyl ether, and shaking to dissolve until the solution is transparent to obtain an organic phase;

2) removing the ether from the organic phase in the step 1) at constant temperature of 40 ℃ under reduced pressure until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10min to obtain a dried substance;

3) dissolving Ascophyllum Nodosum extract, mevalonolactone and hypecol callus culture filtrate in phosphate buffer solution with pH of 6.0 to obtain water phase effective components;

4) adding the water phase functional components obtained in the step 3) into the dried substance obtained in the step 2), and performing normal-pressure rotation oscillation hydration and demoulding at the temperature of 35 ℃;

5) and (3) carrying out ultrasonic treatment for 5-20min after complete membrane removal, and filtering to obtain the compact skin effect liposome with uniform particle size.

Preferably, the lecithin is soybean lecithin, and the mass ratio of the lecithin to cholesterol is (2-5): 1;

the mass ratio of the dunaliella salina extract, the mevalonolactone and the hypsizygus marmoreus callus culture filtrate to the lecithin is 1 (1-3): 0.5-1.5): 20-30;

the volume ratio of the water phase to the organic phase is 1 (2-5).

Preferably, the prepared liposome has a particle size of 0.1-0.5 μm.

In addition, the invention also discloses a preparation method of the toning lotion composition with the skin tightening effect liposome, which comprises the following process steps:

1) adding water, chelating agent, humectant, thickener, part of antiseptic, and pH regulator into water phase pot, stirring and heating to 80-85 deg.C, keeping the temperature and stirring for 20-25 min;

2) boiling cooling water, stirring, cooling to 40-45 deg.C, adding the composition liposome with skin tightening effect, skin conditioner, solubilizer, essence and other antiseptic, adding into emulsifying pot, and stirring for 15-20 min;

3) and continuously stirring and cooling until the temperature is lower than 38 ℃, stopping stirring, and discharging after the detection is qualified.

Further, the cosmetic water composition containing the skin-firming effect liposome is prepared by dispersing or suspending the cosmetic water composition in a solvent, or is prepared by emulsifying particles or is prepared by coating a polymer.

Specifically, the cosmetic containing the liposome with the skin tightening effect is a skin care product, in particular emulsion, essence, cream, eye cream, body lotion, gel and sun block lotion.

By means of the technical scheme, the invention has the following advantages and beneficial technical effects:

1) the invention contains a variety of effective components with skin tightening effect, such as the extract of the Ascophyllum nodosum, the mevalonolactone and the hypsizygus marmoreus callus culture filtrate, and can tighten skin through the synergistic effect of a plurality of ways; the invention selects the extract of the Ascophyllum nodosum, the mevalonolactone and the hypsizygus marmoreus callus culture filtrate, prepares the composite of the carriers acceptable for cosmetics by a liposome preparation technology, finds the most preferable composition proportion by the preferable combination and the efficacy test, and plays the best effect of tightening the skin;

2) the toning lotion disclosed by the invention contains the liposome with the skin tightening effect, and all the components are mutually synergistic, so that the safe and effective skin tightening effect is achieved. The liposome of the efficacy composition prepared from the three efficacy components can be added into cosmetics to promote transdermal absorption, effectively act on two skin layers of skin epidermis and dermis, enhance skin elasticity and tighten skin.

3) The innovative skin-tightening effect lotion has the advantages that the effect components exist in the form of the liposome, can permeate into the deep layer of the skin, directly and effectively supplement nutrition to the skin, stimulate the regeneration of epidermis and the synthesis of collagen, promote the self-generation of cholesterol in the skin, effectively maintain the elasticity of the skin, capture free radicals, repair the damaged skin and tighten the skin.

4) The skin firming lotion disclosed by the invention achieves a safe and effective skin firming effect finally by scientifically combining the raw materials and utilizing the synergistic effect among the functional components, so that the skin is full of elasticity, and the young skin state is kept. The various functional components have the effects of promoting cell renewal of epidermis and dermis, controlling inflammation, stimulating the synthesis of fibers in extracellular matrix, preventing ultraviolet injury, etc.

Drawings

FIG. 1 is the skin wrinkle Ra value after use of example 1;

FIG. 2 is the F4 value for skin firmness after use of example 1;

FIG. 3 is the R2 values for skin elasticity after use of example 1;

FIG. 4 is a clinical evaluation of external canthus wrinkle score by a dermatologist after using example 1;

FIG. 5 is the smoothness score of the skin clinically evaluated by the dermatologist after use of example 1;

FIG. 6 is a dermatologist clinical assessment of the full-face fine-grain score after using example 1;

FIG. 7 is a clinical assessment of skin firmness scores by a dermatologist after using example 1.

Detailed Description

The invention discloses a toning lotion composition of firming skin effect liposome containing multiple firming skin effect components and a preparation method thereof. The skin tightening toning lotion comprises the following raw material components:

0.3-35% of lipidosome with the effect of tightening the skin, 5-30% of humectant, 0-30% of skin conditioner, 0-10% of thickening agent, 0.01-2% of chelating agent, 0.01-1% of solubilizer, a proper amount of pH regulator, a proper amount of preservative, 0.01-1.0% of essence for daily use and the balance of deionized water.

The skin tightening functional component is prepared by mixing a paulownia extract, a methyl-tile-shaped lactone and a hypsizygus marmoreus callus culture filtrate.

(ii) Ascophyllum nodosum extract

Ascophyllum, Phaeophyceae, grows primarily in the North Atlantic Europe and along the Canadian coast. The appearance is slender, the branches are dense and irregular, the edges of the branches are in a shape of blunt saw teeth, the edges of the branches have organs like roots, stems, leaves and the like, the branches are in a brown green color, and a nutrition bubble is arranged in the middle of the branches. The sea water has tenacious vitality, grows on rocks beside the sea or on the sea bottom, has strong enrichment and absorption capacity, can enrich the nutrition in the sea water, and metabolizes and synthesizes dozens of bioactive substances such as seaweed polysaccharide, mannitol, phenols, alginate, brown algae polyphenol, laminarin, fucoidin, cytokinin, gibberellin and the like.

The Ascophyllum nodosum extract has various effects on skin care, and can help to accelerate cell renewal of epidermis and dermis layers and stimulate skin regeneration; can resist the damage of UVB radiation to the skin and promote cell metabolism; preventing the leather fiber from deteriorating and reducing the generation of wrinkles; regulating cytokine, controlling inflammation, and promoting skin repair.

The preparation method comprises the following steps:

1) pretreatment of raw materials: taking fresh soaked-leaf algae as a raw material, removing impurities such as straw sticks, silt and the like attached to the algae leaves, soaking in a 75% ethanol solution for 15-30 min, and airing for later use;

2) grinding, crushing and extracting: adding a small amount of liquid nitrogen into the raw materials in the step 1), grinding, crushing by using a tissue triturator, and extracting for 12 hours at the temperature of 30 ℃ by using 90% ethanol as an extraction solvent;

3) filtering and distilling under reduced pressure: quickly filtering the extract obtained in the step 2) by using a Buchner funnel, and distilling the filtrate under reduced pressure to remove ethanol so as to obtain the extract of the Ascophyllum nodosum.

② methyl tile acid lactone

The mevalonolactone is extracted from "Saccharomycopsis fibuligera". When it is put into water, that is, into a formula, it is immediately turned into "mevalonic acid", which is a substance that the human body itself contains. Healthy human skin requires 3 kinds of intercellular lipids, and stable connection of the intercellular lipids is essential for skin health. Among the three intercellular lipids, "cholesterol" is reduced with the age, and the emamectin benzoate is an indispensable intermediate for the human body to synthesize cholesterol, supplements the cholesterol which is needed by the emamectin benzoate and can well stimulate the skin to synthesize by itself, solves various problems of the skin from a deeper layer, effectively maintains the skin thickness and keeps the skin elasticity.

③ Illicium henryi callus culture filtrate

The sea fennel callus culture filtrate is derived from plant stem cells extracted from the root of the sea fennel callus culture, and the stem cells contain phenolic acid, chlorogenic acid, flavonoid and the like to be on the dermal layer, and can stimulate the synthesis of fibers in extracellular matrix, such as collagen, elastin and proteoglycan.

At the same time, it also helps to balance the production of keratinocytes and epidermal regeneration in terms of cell size, both skin layers can be more densified and globally balanced. The hypsizygus marmoreus callus culture filtrate is beneficial to compacting dermis and tightening skin. Is helpful for improving or restoring dermal function and enhancing skin resistance.

The preparation method comprises the following steps:

1) cleaning fresh sea fennel roots and stems with clear water, airing, soaking for 30s with 75% ethanol solution, soaking for 60 min with 25.0g/L sodium hypochlorite disinfectant, and cleaning with sterile water to remove disinfectant residues;

2) cutting the sterile explant obtained in the step 1) into 1cm long strips, inoculating the long strips into a callus induction culture medium for culture, placing the culture medium in a culture room with the temperature of 22-26 ℃ and the illumination intensity of 1800-2000 LX, illuminating for 12h/d, and culturing for 20-25d to induce callus;

3) selecting yellow green loose callus, and pulverizing into 0.5cm²Inoculating the small blocks into a subculture medium for subculture, and continuously subculturing for 4 times by taking 20d as a period to obtain callus which grows vigorously, has small particles and is loose, wherein the subculture conditions are the same as the induction culture conditions in the step 2);

4) crushing the callus in the step 3), adding 60-100mg/kg of cellulase for treating for 3-5 hours, filtering by a 100-micron microporous filter, centrifuging at low speed, removing supernatant, collecting seed cells, inoculating the seed cells into a cell suspension culture medium, wherein the inoculation amount is 50g/L, the rotating speed is 100r/min, the temperature is 25 ℃, performing shaking culture, and stopping the culture when the cell count is more than 20000/mL;

5) inoculating the cultured cells in step 4) into MS culture medium, adding auxin IAA 5-10mg/L and pectase 50-100mg/L, performing suspension culture in bioreactor at stirring rate of 100r/min and pH of 5-6 at 0.03m³Introducing sterile oxygen gas/s, culturing in dark for 20-30 days, and stopping culturing when the cell count is above 45000/mL;

6) centrifuging and collecting to obtain hypecoum vulgare callus culture filtrate.

Wherein, the callus induction culture medium and the subculture medium in the steps 2) and 3) are solid culture media, and the formulas are respectively as follows: MS +2.5 mg/L2, 4-D +2.5 mg/L6-BAA +5g/L vitamin C +30g/L sucrose +7g/L agar, pH 5.5 and MS +2.5 mg/L2, 4-D +3.0mg/L ZT, pH 5.8. The cell suspension culture medium in the step 4) is a liquid culture medium, and the formula of the cell suspension culture medium is as follows: MS culture medium +1.5 mg/L2, 4-D +0.5 mg/L6-BA +200mg/L hydrolyzed casein +40g/L sucrose, and pH value is 5.8.

The toning lotion composition of the skin tightening effect liposome is composed of the ascophyllum nodosum extract, the mevalonolactone and the hypsizygus marmoreus callus culture filtrate, and nourishes the skin through the composition to form a skin barrier, resist the damage of environmental pollutants to the skin, enhance the toughness of the dermis, prevent and repair the skin photodamage, promote the synthesis of collagen and lipid, maintain the skin elasticity and have the skin tightening effect.

Normally, the skin can only absorb some electrolytes, small molecular substances and fat-soluble substances, wherein the stratum corneum is the most important path for skin absorption. The skin-tightening efficacy composition liposome can easily penetrate through the stratum corneum, and water-soluble macromolecule efficacy ingredients are carried to the deep layer of the skin, so that the skin-tightening effect is better exerted.

The present invention is illustrated by the following examples, which are provided for the purpose of further illustration only and are not intended to limit the scope of the present invention. Other insubstantial modifications and adaptations of the present invention can be made without departing from the scope of the present invention.

Unless otherwise specified, the following percentages (%) are mass percentages. All the raw material components used in the invention are commercial products.

Examples 1 to 4

A cosmetic water composition containing liposome with skin tightening effect is provided, and the raw material components of examples 1-4 have the proportion contents shown in the following table 1.

Table 1 examples 1 to 4 raw material composition ratios (unit: mass%)

The method for preparing the toning lotion composition with the skin tightening effect of the embodiments 1 to 4 of the invention can be expressed as follows:

1) adding water, allantoin, EDTA-disodium, citric acid, sodium citrate, glyceryl polyether-26, glycerol, glyceryl acrylate/acrylic acid copolymer, 1, 3-propylene glycol, polyethylene glycol-32, sodium polyacrylate and methyl hydroxybenzoate into a water phase pot, stirring and heating to 80-85 deg.C, maintaining the temperature and stirring for 20-25 min;

2) adding cooling water, stirring, cooling to 40-45 deg.C, adding tightening skin effect composition liposome, sodium lactate, Hamamelis virginiana extract, polysorbate-60, essence, and phenoxyethanol, adding into emulsifying pot, and stirring for 15-20 min;

Specifically, the preparation method of the liposome of the firming skin effect composition of example 1 comprises the following steps:

1) dissolving 1680mg of lecithin and 480mg of cholesterol in 40mL of diethyl ether, and shaking for dissolution until the solution is transparent to obtain an organic phase;

2) removing the ether from the organic phase in the step 1) at constant temperature of 40 ℃ under reduced pressure until a layer of transparent film is formed on the inner side of the bottle wall, and drying for 10 min;

3) dissolving 60mg of Ascophyllum nodosum extract, 120mg of mevalonolactone and 60mg of Criste anisetree callus culture filtrate in 15mL of phosphate buffer solution with pH of 6.0 to obtain water phase effective components;

Specifically, the preparation method of the skin tightening efficacy composition liposome of the embodiment 2 comprises the following process steps:

1) dissolving 1260mg of lecithin and 360mg of cholesterol in 30mL of diethyl ether, and shaking for dissolving until the solution is transparent to obtain an organic phase;

3) dissolving 45mg of Ascophyllum nodosum extract, 90mg of mevalonolactone and 45mg of Criste anisetree callus culture filtrate in 10mL of phosphate buffer solution with pH of 6.0 to obtain water phase effective components;

Specifically, the preparation method of the skin tightening efficacy composition liposome of the embodiment 3 comprises the following process steps:

1) dissolving 840mg of lecithin and 240mg of cholesterol in 25mL of diethyl ether, shaking and dissolving until the solution is transparent, and obtaining an organic phase;

3) dissolving 30mg of Ascophyllum nodosum extract, 60mg of mevalonolactone and 30mg of Criste anisetree callus culture filtrate in 8mL of phosphate buffer solution with pH of 6.0 to obtain water phase effective components;

Specifically, the preparation method of the liposome of the composition for tightening skin of example 4 comprises the following steps:

1) dissolving 420mg of lecithin and 120mg of cholesterol in 20mL of diethyl ether, and shaking for dissolving until the solution is transparent to obtain an organic phase;

3) dissolving 15mg of Ascophyllum nodosum extract, 30mg of mevalonolactone and 15mg of Criste anisetree callus culture filtrate in 6mL of phosphate buffer solution with pH of 6.0 to obtain water phase effective components;

Test example for Effect verification

Wrinkle removal Effect test 1

1. The tester: 31 healthy persons (25-55 years old), female: fine lines and dry lines exist on the forehead and the cheek, the facial skin is lack of elasticity, and the face is not obviously reddened, damaged, scarred and the like; the example 1 formulation was tried.

2. Testing parts: the entire face (including the periocular region).

3. Testing an instrument: primos.

4. Testing indexes are as follows: a decrease in the analytical value Ra indicates an improvement in skin wrinkles.

5. The test results are shown in fig. 1 and table 2 below:

TABLE 2 skin wrinkle Ra value after use of example 1

Skin wrinkle Ra value	Before use	After 7 days of use	After 14 days of use	After 28 days of use
					Forehead head	26.02	24.68	24.39	24.05
Cheek	19.42	19.01	18.83	18.26

As can be seen from the results of fig. 1 and table 2, compared to before using the sample: after 7 days of sample use, the forehead skin wrinkle Ra value was significantly reduced by 5.15%, and the cheek skin wrinkle Ra value was significantly reduced by 2.11%; after 14 days of sample use, the forehead skin wrinkle Ra value was significantly reduced by 6.26%, and the cheek skin wrinkle Ra value was significantly reduced by 3.04%; after 28 days using the sample, there was no significant reduction in forehead skin wrinkle Ra value by 7.57%, and a significant reduction in cheek skin wrinkle Ra value by 5.97%.

The skin wrinkle Ra value was significantly reduced after use of example 1, indicating that the firming lotion of the present invention has a relatively significant wrinkle removal effect.

Skin tightening effect test 2

2. Testing parts: the entire face (including the periocular region).

3. Testing an instrument:

MPA580。

4. testing indexes are as follows: the reduced F4 value for skin firmness indicates that the skin is firmer.

5. The test results are shown in fig. 2 and table 3.

Table 3 skin tightening F4 values after use of example 1

Skin firming F4	Before use	After 7 days of use	After 14 days of use	After 28 days of use
					Forehead head	5.35	5.28	5.12	5.06
Cheek	5.15	5.03	4.95	4.81

As shown in fig. 2 and table 3, compared to before using the sample: after 7 days of sample application, the forehead skin tightening F4 value was significantly reduced by 1.31%, and the cheek skin tightening F4 value was significantly reduced by 2.33%; 14 days after application of the sample, the forehead skin firmness F4 value was significantly reduced by 4.30%, and the cheek skin firmness F4 value was significantly reduced by 3.88%; after 28 days of use of the sample, there was a significant reduction of 5.42% in the forehead skin firmness F4 value and 6.60% in the cheek skin firmness F4 value.

The above data show that the F4 value for forehead and cheek skin tightening is significantly reduced after example 1 is used, indicating that the tightening efficacy lotion of the present invention has a relatively significant skin tightening effect.

Skin elasticity effect test 3

1. The tester: 30 healthy persons (25-55 years old), female: fine lines and dry lines exist on the forehead and the cheek, the facial skin is lack of elasticity, and the face is not obviously reddened, damaged, scarred and the like; the example 1 formulation was tried.

2. Testing parts: the entire face (including the periocular region).

3. Testing an instrument:

MPA580。

4. testing indexes are as follows: the measurement increases and closer to 1, indicating better skin elasticity.

5. The test results are shown in fig. 3 and table 4 below:

table 4 skin elasticity R2 values after use of example 1

The results in fig. 3 and table 4 show that, compared to before the use of the sample: after 7 days of using the sample, the forehead skin elasticity R2 value has no significant increase of 1.69%, and the cheek skin elasticity R2 value has a significant increase of 3.70%; after 14 days of sample application, the forehead skin elasticity R2 value was significantly increased by 6.78%, and the cheek skin elasticity R2 value was significantly increased by 9.26%; after 28 days of sample use, there was a significant 10.17% increase in the forehead skin elasticity R2 value and a significant 14.81% increase in the cheek skin elasticity R2 value.

The above results demonstrate that the lotion of the present invention can significantly increase skin elasticity.

Dermatologist clinical assessment 4

1. Evaluation subject: 33 healthy persons (25-55 years old), female: fine lines and dry lines exist on the forehead and the cheek, the facial skin is lack of elasticity, and the face is not obviously reddened, damaged, scarred and the like; the example 1 formulation was tried.

2. Evaluation site: the entire face (including the periocular region).

3. Evaluation indexes are as follows: wrinkles, fine texture, fine lines on the whole face, firmness of the skin.

4. Grading standard: wrinkling: score 0 indicates no wrinkles, score 9 indicates very deep wrinkles; smoothness of skin: 0 score indicates very rough skin, and 9 score indicates very fine skin; fine lines of the whole face: score 0 indicates many visible lines, score 9 indicates that almost no lines were observed; firmness of the skin: a score of 0 indicates that the skin is not tight (slack skin) and is easily deformed by stretching but not easily returned to its original state, and a score of 9 indicates that the skin is very tight, has significant resistance to pulling or pressure, and is easily returned to its original state.

5. And (4) evaluation results: as shown in fig. 4-7 and table 5.

TABLE 5 clinical assessment score for dermatologist

Mean clinical score	Before use	After 14 days of use	After 28 days of use
				External canthus wrinkle	2.85	2.43	2.01
Fine and smooth skin texture	4.77	5.39	6.32
				Fine lines of whole face	4.79	5.19	5.90
Firmness of skin	4.45	5.00	5.58

Compared to before using the sample: after the sample is used for 14 days, the clinical score mean of external canthus wrinkles is reduced by 14.74%, the fine clinical score mean of skin texture is increased by 12.84%, the clinical score mean of full-face fine lines is increased by 8.42%, and the firmness clinical score mean of skin is increased by 12.32%; after the sample is used for 28 days, the clinical score mean of external canthus wrinkles is reduced by 29.48 percent, the fine clinical score mean of skin texture is increased by 32.43 percent, the clinical score mean of full-face fine lines is increased by 23.23 percent, and the tightening clinical score mean of skin is increased by 25.36 percent.

Clinical evaluation results of dermatologists show that the astringent with the tightening effect has remarkable effects on four aspects of reducing external canthus wrinkles, improving skin fineness, smoothing whole face wrinkles and improving skin tightness.

Safety test 5

1. The number of tested persons: 30 healthy people, 13 men and 17 women; the age range is 20-45 years.

2. The test method comprises the following steps: the selection area is not more than 50mm²In a suitable patch tester having a depth of about 1mm, about 0.020 to 0.025mL (g) of the test substance of example 1 was added to the patch tester by the closed patch test method. The patch tester is applied to the curved side of the forearm of the subject, the subject is removed 24 hours later, the skin reaction is observed 0.5 hour, 24 hours and 48 hours after the removal, and the result is recorded according to the skin reaction grading standard in the technical Specification for cosmetic safety 2015.

3. The evaluation criteria are as follows in table 6:

TABLE 6 grading Standard of adverse skin reactions

The results show that: the result of the human skin patch experiment shows that the score grades of 0.5h, 24h and 48h of 3 observation time points after 30 subjects remove the patch are all 0, namely 0 of 30 subjects have positive reaction, and the subject does not cause adverse skin reaction to the subjects in the batch according to the regulation in technical Specification for cosmetic safety (2015 edition).

Synergistic test example 6

A toning lotion composition with skin tightening effect comprises the raw material components of comparative examples 1-7 according to the proportion, and is shown in the following table 7.

Comparative examples 1 to 7

TABLE 7 COMPARATIVE EXAMPLES 1 to 7 raw material composition ratio (% by mass)

The manufacturing process of the toning lotion with skin tightening effect of comparative examples 1 to 7 of the present invention can be expressed as follows:

2) adding cooling water, stirring, cooling to 40-45 deg.C, adding tightening skin effective component, sodium lactate, Hamamelis virginiana extract, polysorbate-60, essence, and phenoxyethanol, adding into emulsifying pot, and stirring for 15-20 min;

The skin firmness test was performed on the face before and after using example 1 and comparative examples 1 to 7, and the test results are shown in tables 8 to 9.

Table 8 forehead skin firmness F4 values before and after use in example 1 and comparative examples 1-7

Table 9 example 1 and comparative examples 1-7 cheek skin tightening F4 values before and after use

Use of

MPA580 tests the skin tightness F4 value, with a decrease in F4 value indicating a more tight skin. It can be seen from the data in tables 11-12 that the rate of reduction of the forehead skin firmness F4 values after use of example 1 and comparative examples 1-7 is ranked by magnitude: example 1>Comparative example 1>4>3>2>6>5>7; the cheek skin tightness F4 values were ranked by size as: example 1>Comparative example 1>4>2>3>5>6>7。

In conclusion, the compound with the effects of improving skin color and brightening has the effects of remarkably improving skin color and brightening through the synergistic effect of the components, and the effect of improving skin color is more obvious than that of the combination of two or three components.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, so that any simple modification, equivalent change and modification made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.

Claims

1. A toning lotion composition containing a skin-firming effect liposome, which is characterized in that: the composition comprises the following raw material components in percentage by weight:

0.05-35.0% of a firming skin efficacy composition liposome comprising: 0.01-30% of Ascophyllum nodosum extract, 0.01-30% of methyl-tile lactone and 0.01-30% of hypsizygus marmoreus callus culture filtrate;

5-30% of humectant;

0-30% of a skin conditioning agent;

0-10% of a thickening agent;

0.3-5.0% of preservative;

0.01-2% of a chelating agent;

0.01-1% of a solubilizer;

0.01-1.0% of daily essence;

proper amount of pH regulator and deionized water.

2. The emollient water composition comprising liposomes for tightening skin efficacy according to claim 1 wherein: the composition comprises the following components in percentage by weight based on the total weight of the composition:

0.2-25.0% of a firming skin efficacy composition liposome comprising: 0.05-20% of Ascophyllum nodosum extract, 0.05-20% of methyl-tile lactone and 0.05-20% of Craib anisetree callus culture filtrate;

3. the emollient water composition comprising liposomes for tightening skin efficacy according to claim 1 wherein: the firming skin effect composition liposome comprises:

4. The emollient water composition comprising liposomes for tightening skin efficacy according to claim 3 wherein: the firming skin effect composition liposome comprises:

5. The emollient water composition comprising liposomes for tightening skin efficacy according to any of claims 1 to 4 wherein:

the humectant is selected from one or more of glycerin, propylene glycol, 1, 3-propylene glycol, glycerin acrylate/acrylic acid copolymer, glyceryl polyether-26, hexylene glycol, 1, 2-pentanediol, caprylyl glycol, ethylhexyl glycerin, polyethylene glycol-32, urea, allantoin, PEG/PPG-17/6 copolymer, PPG-28-buthyl polyether-35, bis-PEG-18 methyl ether dimethylsilane, sorbitol, and combinations thereof;

the solubilizer is selected from one or more of PEG-60 hydrogenated castor oil, polysorbate-20 and polysorbate-60;

6. The emollient water composition comprising liposomes for tightening skin efficacy according to claim 1 wherein: the preparation process of the composition liposome with the skin tightening effect comprises the following steps:

5) and (4) carrying out ultrasonic treatment for 5-20min after complete membrane removal, and filtering to obtain the compact skin effect composition liposome with uniform particle size.

7. The emollient water composition comprising liposomes for tightening skin efficacy according to claim 6 wherein:

the lecithin is soybean lecithin, and the mass ratio of the lecithin to cholesterol is (2-5) to 1;

the volume ratio of the water phase to the organic phase is 1 (2-5).

8. The emollient water composition comprising liposomes for tightening skin efficacy according to claim 6 wherein: in the step 5), the particle size of the prepared liposome is 0.1-0.5 μm.

9. The method of preparing a astringent composition containing tight skin efficacy liposomes according to any one of claims 1 to 8, comprising the steps of: