CN109771322A - Human stem cell factor skin repair composition and its application in skin care item - Google Patents
Human stem cell factor skin repair composition and its application in skin care item Download PDFInfo
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- CN109771322A CN109771322A CN201910206718.9A CN201910206718A CN109771322A CN 109771322 A CN109771322 A CN 109771322A CN 201910206718 A CN201910206718 A CN 201910206718A CN 109771322 A CN109771322 A CN 109771322A
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Abstract
The invention discloses a kind of human stem cell factor skin repair composition and its applications in skin care item, the human stem cell factor skin repair composition is prepared by the raw material of following mass percents: human stem cell growth 0.00001-0.00002%, bringing back to life careless extract 40-50%, surplus is birds nest extract.Porcine HGF is the optimum activity ingredient of skin nursing in the present invention, it can hinder through skin surface cell and be absorbed by deep skin cell, primordial stem cell effectively activating basal layer, no longer breaking up, enhances the division growth ability and metabolic function of Skin Cell；Simultaneously containing bird's nest, the careless essence of resurrection, it is safe, non-stimulated, have both moisturizing, whitening spot-removing, crease-resistant effect, raw material of the present invention is mildly safe, skin is not damaged, manufactured skin care item have excellent anti-oxidant, moisturizing, penetrating power, effectively remove the free radical in skin, deep skin can be infiltrated into, plays reparation, anti-oxidant, moisturizing water lock effect from deep layer cells.
Description
Technical field
The invention belongs to technical field of skin care, specifically, being related to a kind of human stem cell factor skin repair composition
And its application in skin care item.
Background technique
Porcine HGF plays biological regulation effect to various kinds of cell physiological function and metabolic activity, they are in vivo
Content is atomic, but bioactivity is high.In beauty and skin care, plastic surgery, burn ulcer and various dermopathic wound reparations
It plays an important role with healing.
Porcine HGF is combined with the specific receptors being present on target cell and is played a role, principal biological effect
It answers are as follows: chemotactic induction inflammatory cell, promotes target cell to synthesize extracellular matrix secretion, such as glue at stimulation target cell proliferation and differentiation
Original etc..Cell growth factor regulations T cell, B cell, histocyte, the formation of Langerhans' cells and corium dendron, development,
The functions such as metabolism, and influence their migration and growth rate.Connective tissue, keratinocyte, fibroblast are also stimulated simultaneously
With secrete cytokines, lymphokine, haemocyanin, extracellular protein and the matrix polysaccharide such as macrophage.In embryonic development period, carefully
The intracellular growth factor participates in the adjusting of histoorgan formation by adjusting the activity of identical or different cell type；For developing into
Ripe histoorgan, the impaired organ caused after wound, function weaken, and cell factor has significant reconstruction function.
During the skin aging due to caused by the factors such as age, physiology, environment, skin activity is reduced, under physiological function
Drop, the serious pathology that also will cause sexually revise.Skin is subdivided into the shallow relatively thin epidermis of table, the corium containing more multifilament and subcutaneous
Tissue.Epidermis is differentiated by the ectoderm for being covered in embryonic surface, is made of multilayer scaly epithelium, from thick scalp to complete
The most thin eyelid skin of body (0.04mm), thickness are each different.Epidermis can be subdivided into epithelial cells, dentritic cell again
With Michael cell；Corium mainly from mesoderm development, is located under epidermis, by one layer of basement membrane zone point between epidermis
It opens.Corium is mainly made of connective tissue, blood vessel, nerve, muscle, lymph, sweat gland and sebaceous glands is also included, shallowly relatively thin
Referred to as papillaris pars, and deep layer it is thicker then be referred to as lamina reticularis.The main component of corium is collagen, and collagenous fibres are in papillaris pars
Content but is then in that pencil is parallel to surface alignment in network layer seldom and irregular arrangement；Subcutaneous tissue or adipose membrane are body surfaces
Fascia superficialis, be made of loose connective tissue and adipose tissue.Corium stretches out many collagens to differ in size in subcutaneously organizing
Fibre bundle connect skin securely with subcutaneous tissue, between the two without obvious boundary.In the form of molecule or pass through special biography
Delivery system (liposome, package liposome, microballoon), is added to facial cleanser, gel, essence or lotion for Porcine HGF
In, important Molecular biological function is played ageing skin.The effect of Porcine HGF is: 1. on epidermis level: shadow
The activity and growth factor of keratinocyte are rung, stimulates keratinocyte migration and epithelialization, stimulation epidermal cell breaks up and correction, by force
Strong promotion epidermis reparation and healing；2. on dermal level: stimulation fibroblasts, enhancing extracellular matrix is shunk and structure
It makes, skin Nutrients uptake skin wound healing and functional regeneration is provided；3. in skin integral level: enhancing to environmental assaults,
The resistance of ultraviolet light, pollutant, stimulant, sensitizer, inflammatory cell is consolidated, enhancing skin tension, enhances skin elasticity,
It stimulates cicatricial tissue to fade, reduces cicatrization, delay skin aging.Porcine HGF main mechanism has: 1. promoting epidermis
With corium confluent monolayer cells (especially fibroblast, epithelial cell, endothelial cell) proliferation, division, differentiation, neonatal cell increases,
Make pachyderma, is gradually recovered skin normal configuration and physiological function；2. promotion collagenous fibres, reticular fibre, elastomer
It is formed, adjusts the secretion of collagen and glutinous polysaccharide, maintain moisture content and electrolyte metabolism in skin histology, improve atrophy skin
The exsiccosis of skin moisturizes the skin tissue；3. promoting skin histology constantly to be formed newly by the effect to vascular endothelial cell
Capillary.
Up to the present, the Porcine HGF that research is found has tens kinds, damages with beauty and skin care and skin soft tissue
Repairing in close relations mainly has:
Insulin-like growth factor-i (IGF-1) Human Umbilical Vein Endothelial Cells play chemotaxis and DNA are stimulated to synthesize, it can accelerate
The division and proliferation of different type cell increase the synthesis of protein and steroids, in wound repair and nerve cell are rebuild
It works.
Acid fibroblast growth factor (aFGF) is a kind of from the multi-functional thin of mesoderm and neuroderm
Intracellular cytokine is a kind of extremely strong mitogen of effect.It can increase point of cartilage cell, sarcoblast and vascular endothelial cell
Change, induce formation, the growth and development of capilary, promotes wound repair.
The biological function of basic fibroblast growth factor (bFGF) is similar to aFGF, to vascular endothelial cell, at
Fibrocyte, sarcoblast, osteoblast etc. have rush splitting action.
Epithelical cell growth factor (EGF) can promote turning for the small-molecule substances such as K+, deoxyglucose, α-aminoacid
Fortune increases the synthesis and secretion of extracellular matrix, promotes the synthesis of RNA, DNA and protein.EGF can increase after being applied to the surface of a wound
The content of DNA, RNA, hydroxyproline, proline, protein and extracellular macromolecular substances in granulation tissue promote on wound
Pi Hua shortens wound healing time, enhances wound tissue's Tensile strength.
Keratinocyte growth factor (KGF) is most important influence factor in keratinocyte and hair follicle forming process, stimulation
The synthesis of DNA promotes the growth for maintaining human keratinocyte, epidermal cell, epithelial cell.KGF dysfunction can lead to epidermis and wither
Contracting, hair follicle exception and corium are impaired.KGF plays an important role in adjusting epidermal keratinocytes proliferation and wound healing process.
In recent years, deepening continuously with organizational engineering research, human stem cell growth is by unprecedented heavy
Depending on.It can through skin surface cell hinder and be absorbed by deep skin cell, effectively activate basal layer, no longer break up
Primordial stem cell, enhance the division growth ability and metabolic function of Skin Cell.The present invention is directed to develop people in one
Stem cell factor skin repair composition and its application in skin care item.
Summary of the invention
Of the existing technology in order to solve the problems, such as, one of the object of the invention is to provide a kind of human stem cell factor skin and repairs
Compound composition and its application in skin care item can control or regulate skin aging process, protect damaged skin.
The purpose of the present invention is what is be accomplished by the following way:
A kind of human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: people is dry thin
Intracellular growth factor 0.00001-0.00002%, careless extract 40-50% is brought back to life, surplus is birds nest extract.
Human stem cell growth (human stem cell growth factor, hSCGF also known as LSLCL) is a kind of
The early stage hematopoiesis stimulating factors to work in marrow stromal cell have multiple biological activities.Its assignment of genes gene mapping Yu Renran
Colour solid 19q13.3 is made of 4 exons and 3 intrones.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 10-50 mesh for grass is brought back to life, by smashed resurrection
Grass, volume fraction be that 75-85% ethanol water is 1:(10-20 in mass ratio) mixing after ultrasonic extraction 30-60min, filtering,
Being concentrated into water content is 5-10wt%, obtains bringing back to life careless extract.
Careless (Anastatica) is brought back to life, is a kind of desert plant, also referred to as " rose of Jericho ", since it can be
In the case where almost being dried completely " resurrection " and it is well-known.Since before remote 300,000,000 years, just grow on earth climing
Prolong, and rely on itself exclusive divinity, withstands on the earth the extremely serious dry spell several times, tenaciously multiply so far.
The strength why grass possesses such mystery is brought back to life in desert, the life miracle assigned from the Nature: the extreme drought the case where
Under, its branches and leaves can tightly shrink glomeration, firmly lock precious in vivo last and drip, even if losing itself is up to 98%
Moisture, still can indomitable existence many decades.Once encountering rare rainy season, brings back to life in cursive script and contain glycosyl trehalose abundant just
Can play powerful water lock moisture-keeping functions, " greediness " " grabbing " live it is each drip, then will contain the water of vitality smoothly again
It is living freely to import in each cell in vivo, and latching is equivalent to 460 times of cell own wt of moisture, as " coming back to life "
Re-expand branches and leaves, restore full of life green life.
Further, it is described bring back to life careless extract the preparation method comprises the following steps: crushed 10-50 mesh for grass is brought back to life, will crush
Carbon dioxide supercritical extraction 1-4h is carried out in the extraction kettle of resurrection grass investment supercritical extraction unit afterwards, obtains overcritical extraction
Take object；By after supercritical extract resurrection grass, phenol antioxidant, volume fraction be 75-85% ethanol water in mass ratio
For ultrasonic extraction 30-60min after 1:(0.002-0.009): (10-20) mixing, filtering, being concentrated into water content is 5-10wt%,
Obtain sonicated extract；It obtains bringing back to life careless extract after supercritical extract, sonicated extract are mixed.
The ultrasonic power of the ultrasonic extraction is 200-500W, supersonic frequency 30-50kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 30-40 DEG C, extracting pressure 25-35MPa, carbon dioxide
Flow is 10-30kg/h, separation temperature is 28-32 DEG C, separating pressure 6-9MPa.
The phenol antioxidant is Cranberry polyphenol derivatives, at least one in green peel of walnut phenol natural antioxidant
Kind.Further, the phenol antioxidant is Cranberry polyphenol derivatives, green peel of walnut phenol natural antioxidant by quality
Than being formed for 1:(0.2-0.3).
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 40-100 mesh, smashed bird's nest, water are pressed
Mass ratio is 1:(5-15) it mixes and 2-4h is stirred for 100-300r/min with revolving speed, be then in pressure in addition high pressure homogenizer
40-60MPa carries out high-pressure homogeneous processing 2-3 times, and the time per treatment is 5-10min, obtains homogenization liquid；By homogenization
Liquid first uses 500-1000 mesh filter-cloth filtering, then uses the ultrafiltration membrane ultrafiltration of 1000-10000Da, obtains filtrate；By filtrate in temperature
Be 10-50Pa vacuum freeze drying to water content for -35~-55 DEG C, vacuum degree it is 5-10wt%, obtains birds nest extract.
Bird's nest is the nest of swallow.But it is not the nest of common swallow, is a kind of special swallow --- nest of esculent swift.
Apodidae animal esculent swift and a variety of swallow classes that belong to condense built nest with saliva and down etc., and bird's nest is not under direct picking
Come what can be eaten, be need after deep processing can human consumption part.Main place of production Philippine is coastal waste nearby west to Burma
In the cavern on island, with the Southeast Asia such as Indonesia, Malaysia, Sandakan, Singapore and Thailand one with sea area and China south
The island Hai Zhu is in the majority.Bird's nest is also known as delicacy made from bird's nests, Yan Gen, swallow vegetables, for Apodidae animal esculent swift and it is a variety of belong to swallow class with saliva with
Down etc. is mixed and condensed in built up nest, is similar to ingot, and nest outer wall is built by the intensive filiform heap of horizontal stripe into irregular prismatic
Protrusion, nest inner wall are made into irregular netted by filiform, and nest bowl root is solid, the both ends angle You little Zhui, 6~7 centimetres of general diameter,
It is 3~4 centimetres deep.Mainly originate in China South China Sea Islands and Southeast Asian countries.Bird's nest can be divided into three kinds because of acquisition time difference: 1, white
Swallow: ancient times are once classified as tribute, therefore also known as palace swallow；2, Mao Yan；3, blood swallow.The nutrition of bird's nest is higher, contains 50% protein, 30% sugar
Class and some minerals.It is one of rare food of Chinese tradition.Bird's nest modern medical research has found that, bird's nest main component has: water
Soluble proteins, carbohydrate；Microelement: calcium, phosphorus, iron, sodium, potassium and the amino to play an important role to promotion human activity
Acid.
Further, the birds nest extract the preparation method comprises the following steps: bird's nest crushed 40-100 mesh, will be smashed
Bird's nest, modification of chitosan solution are 1:(5-15 in mass ratio) mixing with revolving speed be 100-300r/min stir 2-4h, then plus
Enter in high pressure homogenizer and carry out high-pressure homogeneous processing 2-3 times in pressure for 40-60MPa, the time per treatment is 5-10min, is obtained
To homogenization liquid；Homogenization liquid is first used into 500-1000 mesh filter-cloth filtering, it is then super with the ultrafiltration membrane of 1000-10000Da
Filter, obtains filtrate；By filtrate temperature is -35~-55 DEG C, vacuum degree is that 10-50Pa vacuum freeze drying to water content is 5-
10wt% obtains birds nest extract.
The preparation method of the modification of chitosan solution is: acetic acid is added to the water be made 0.2-1.5wt% acetic acid it is molten
Chitosan, acetum are 1:(480-520 by liquid in mass ratio) it is uniformly mixed, add acetum weight 0.1-0.5%
Cellulase, acetum weight 0.01-0.05% sodium citrate be 50-60 DEG C in temperature at revolving speed for 50-150r/
Min stirs 2-5h, then living in 85-90 DEG C of heat preservation 5-10min enzyme deactivation, obtains modification of chitosan solution.
The present invention also provides application of the above-mentioned human stem cell factor skin repair composition in skin care item.Concrete application
Are as follows: human stem cell factor skin repair composition is added in skin care item by dosage for 5-20wt%.
The present invention also provides a kind of skin care item of composition of skin repair containing human stem cell factor, by mass percentage
Raw material composition are as follows: shea butter 2-5%, vitamin E 0.1-0.5%, hyaluronic acid 0.01-0.1%, glycerol 3-8%, meat
Isopropyl myristate 1-10%, xanthan gum 0.2-2%, bacteriostatic agent 0.01-0.1%, human stem cell factor skin repair composition
5-15%, surplus are water.
The present inventor's stem cell factor skin repair composition, Porcine HGF be skin nursing optimum activity at
Point, can hinder through skin surface cell and be absorbed by deep skin cell, effectively activate basal layer, no longer break up
Primordial stem cell enhances the division growth ability and metabolic function of Skin Cell；Contain bird's nest simultaneously, bring back to life careless essence,
It is safe, non-stimulated, moisturizing, whitening spot-removing, crease-resistant effect are had both, raw material of the present invention is mildly safe, does not damage skin, manufactured shield
Skin product have excellent anti-oxidant, moisturizing, penetrating power, effectively remove the free radical in skin, can infiltrate into deep skin, from
Deep layer cells play reparation, anti-oxidant, moisturizing water lock effect.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, below to specific reality of the invention
The mode of applying is described in detail.In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention.But
The invention can be embodied in many other ways as described herein, and those skilled in the art can be without prejudice to this hair
Similar improvement is done in the case where bright intension, therefore the present invention is not limited by the specific embodiments disclosed below.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
Embodiment is raw materials used as follows:
Chitosan is provided by Xi'an great day biology Engineering Co., Ltd, model: food-grade, deacetylation 90%.
Cellulase is provided by Henan collection beautification chemical product Co., Ltd, model: food-grade, and enzyme activity is 100,000 U/g.
High pressure homogenizer is provided by Shanghai Ke Lao mechanical equipment Co., Ltd, model: KL-GJJ.
Human stem cell growth is according to method system shown in example 1 in the Chinese patent application No. is 201010614697.3
It is standby.
Cranberry polyphenol derivatives are according to method system shown in example 1 in the Chinese patent application No. is 201610210436.2
It is standby.
Green peel of walnut phenol natural antioxidant is according to embodiment 2 in the Chinese patent application No. is 201610285834.0
Shown method preparation.
Bird's nest is esculent swift bird's nest, place of production Malaysia.
Grass is brought back to life to be provided by Meizhou City Shang Shanruoshui Chinese medicine Co., Ltd.
Embodiment 1
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, the careless, body by smashed resurrections
Fraction is that 80% ethanol water is ultrasonic extraction 40min after 1:15 mixing in mass ratio, is obtained using 1000 mesh filter-cloth filterings
To filtrate, by filtrate temperature is 70 DEG C, vacuum degree is that 0.01MPa carries out being concentrated in vacuo to water content being 8wt%, brought back to life
Careless extract.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme, 0.015% sodium citrate of acetum weight be 55 DEG C in temperature at revolving speed be 90r/min stirring 3.5h, then 88
DEG C heat preservation 8min enzyme deactivation is living, obtains modification of chitosan solution.
Embodiment 2
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, volume fraction are that 80% ethanol water is ultrasonic extraction 40min after 1:15 mixing in mass ratio, are adopted
Filtrate is obtained with 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, that vacuum degree is that 0.01MPa be concentrated in vacuo to is aqueous
Amount is 8wt%, obtains sonicated extract；It obtains bringing back to life careless extract after supercritical extract, sonicated extract are mixed.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme, 0.015% sodium citrate of acetum weight be 55 DEG C in temperature at revolving speed be 90r/min stirring 3.5h, then 88
DEG C heat preservation 8min enzyme deactivation is living, obtains modification of chitosan solution.
Embodiment 3
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, phenol antioxidant, volume fraction are that 80% ethanol water is 1:0.005:15 mixed in mass ratio
Ultrasonic extraction 40min after conjunction obtains filtrate using 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, vacuum degree is
0.01MPa carries out being concentrated in vacuo to water content being 8wt%, obtains sonicated extract；Supercritical extract, sonicated extract are mixed
It obtains bringing back to life careless extract after conjunction.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The phenol antioxidant is Cranberry polyphenol derivatives.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme, 0.015% sodium citrate of acetum weight be 55 DEG C in temperature at revolving speed be 90r/min stirring 3.5h, then 88
DEG C heat preservation 8min enzyme deactivation is living, obtains modification of chitosan solution.
Embodiment 4
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, phenol antioxidant, volume fraction are that 80% ethanol water is 1:0.005:15 mixed in mass ratio
Ultrasonic extraction 40min after conjunction obtains filtrate using 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, vacuum degree is
0.01MPa carries out being concentrated in vacuo to water content being 8wt%, obtains sonicated extract；Supercritical extract, sonicated extract are mixed
It obtains bringing back to life careless extract after conjunction.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The phenol antioxidant is Cranberry polyphenol derivatives.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, by smashed bird's nest, deionized water
Be in mass ratio 1:10 mixing with revolving speed be 150r/min stirring 3h, then be added high pressure homogenizer in pressure be 50MPa into
Horizontal high voltage homogenization 3 times, the time per treatment is 6min, obtains homogenization liquid；1000 mesh are first used to filter homogenization liquid
Cloth filtering, then uses the ultrafiltration membrane ultrafiltration of 2000Da, obtains filtrate；By filtrate temperature is -45 DEG C, vacuum degree is 20Pa vacuum
Freeze-drying to water content is 8wt%, obtains birds nest extract.
Embodiment 5
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, phenol antioxidant, volume fraction are that 80% ethanol water is 1:0.005:15 mixed in mass ratio
Ultrasonic extraction 40min after conjunction obtains filtrate using 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, vacuum degree is
0.01MPa carries out being concentrated in vacuo to water content being 8wt%, obtains sonicated extract；Supercritical extract, sonicated extract are mixed
It obtains bringing back to life careless extract after conjunction.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The phenol antioxidant is Cranberry polyphenol derivatives.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme with revolving speed is 90r/min stirring 3.5h at being 55 DEG C in temperature, then living in 88 DEG C of heat preservation 8min enzyme deactivations, obtains modified shell and gathers
Sugar juice.
Embodiment 6
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, phenol antioxidant, volume fraction are that 80% ethanol water is 1:0.005:15 mixed in mass ratio
Ultrasonic extraction 40min after conjunction obtains filtrate using 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, vacuum degree is
0.01MPa carries out being concentrated in vacuo to water content being 8wt%, obtains sonicated extract；Supercritical extract, sonicated extract are mixed
It obtains bringing back to life careless extract after conjunction.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The phenol antioxidant is green peel of walnut phenol natural antioxidant.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme, 0.015% sodium citrate of acetum weight be 55 DEG C in temperature at revolving speed be 90r/min stirring 3.5h, then 88
DEG C heat preservation 8min enzyme deactivation is living, obtains modification of chitosan solution.
Embodiment 7
Human stem cell factor skin repair composition, is prepared by the raw material of following mass percents: human stem cell is raw
The long factor 0.00001% brings back to life careless extract 45%, and surplus is birds nest extract.Human stem cell growth, resurrection grass are mentioned
Object, birds nest extract is taken to be uniformly mixed up to human stem cell factor skin repair composition.
It is described bring back to life careless extract the preparation method comprises the following steps: crushed 30 meshes for grass is brought back to life, by smashed resurrections grass throwing
Enter progress carbon dioxide supercritical extraction 2.5h in the extraction kettle of supercritical extraction unit, obtains supercritical extract；Face super
Boundary's resurrection grass extracted, phenol antioxidant, volume fraction are that 80% ethanol water is 1:0.005:15 mixed in mass ratio
Ultrasonic extraction 40min after conjunction obtains filtrate using 1000 mesh filter-cloth filterings, by filtrate temperature is 70 DEG C, vacuum degree is
0.01MPa carries out being concentrated in vacuo to water content being 8wt%, obtains sonicated extract；Supercritical extract, sonicated extract are mixed
It obtains bringing back to life careless extract after conjunction.
The ultrasonic power of the ultrasonic extraction is 300W, supersonic frequency 40kHz.
The extraction temperature of the carbon dioxide supercritical extraction is 36 DEG C, extracting pressure 30MPa, carbon dioxide flow are
20kg/h, separation temperature be 30 DEG C, separating pressure 8MPa.
The phenol antioxidant is in mass ratio by Cranberry polyphenol derivatives, green peel of walnut phenol natural antioxidant
1:0.25 being mixed to get.
The birds nest extract the preparation method comprises the following steps: bird's nest crushed 80 meshes, smashed bird's nest, modified shell are gathered
It with revolving speed is 150r/min stirring 3h that sugar juice is 1:10 mixing in mass ratio, is then added in high pressure homogenizer and in pressure is
50MPa carries out high-pressure homogeneous processing 3 times, and the time per treatment is 6min, obtains homogenization liquid；Homogenization liquid is first used
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 2000Da, obtain filtrate；By filtrate temperature is -45 DEG C, vacuum degree is
20Pa vacuum freeze drying to water content is 8wt%, obtains birds nest extract.
The preparation method of the modification of chitosan solution is: the acetic acid that 1wt% is made in acetic acid addition deionized water is molten
Chitosan, acetum are uniformly mixed in mass ratio for 1:500 by liquid, add the cellulose of acetum weight 0.15%
Enzyme, 0.015% sodium citrate of acetum weight be 55 DEG C in temperature at revolving speed be 90r/min stirring 3.5h, then 88
DEG C heat preservation 8min enzyme deactivation is living, obtains modification of chitosan solution.
Test case 1
Radical scavenging activity test is carried out to the human stem cell factor skin repair composition of embodiment preparation.Specific knot
Fruit is shown in Table 1.
The preparation of sample liquid: weighing human stem cell factor skin repair composition 5.00g, add deionized water 45.00mL, mixes
It closes uniformly, obtains sample liquid.
Hydroxyl radical free radical (OH) removes experiment: using Fenton-like system Phen-Fe2+Oxidizing process is measured, and is taken
The Phen of 1.5mL 1mmol/L is put into test tube, and 4.5mL 100 is added
The pH7.4 phosphate buffer solution of mmol/L is uniformly mixed, and 1mL 1mmol/L FeSO is added4Solution mixes immediately
It is even, it is separately added into 1mL sample liquid, the H that 1mL mass fraction is 0.1% is then added2O2Aqueous solution starting reaction, keeps the temperature in 37 DEG C
60 minutes, light absorption value is measured at 509nm.Damage pipe is arranged simultaneously (1mL distilled water to be added and replaces sample liquid, 1mL mass fraction is
0.1% H2O2Aqueous solution starting reaction) and do not damage pipe (it is 0.1% that 2mL distilled water, which is added, and replaces sample liquid and mass fraction
H2O2Aqueous solution), wherein damage manages and do not damage pipe using phosphate buffer solution as blank control, chemical feed pipe adds phosphorus with same concentration
The chemical feed pipe of hydrochlorate buffer solution is blank control.Each embodiment operation repetitive 3 times, is averaged.Hydroxyl is calculated as follows
The clearance rate of free radical:
The clearance rate (%) of OH=(A dosing-A damage)/(A does not damage-A damage) × 100.
Table 1: free radical scavenging ability test result table
Test case 2
By embodiment preparation human stem cell factor skin repair composition and other raw material components be prepared into skin care item into
The test of row performance of keeping humidity.
The skin care item of the composition of skin repair containing human stem cell factor are prepared using following methods, comprising the following steps:
(1) 70 parts by weight of deionized water are added in the water phase pot of vacuum homogeneous emulsifying machine with revolving speed as 300r/min stirring
5min is uniformly mixed, and 8 parts by weight of glycerin is then added, 1 parts by weight xanthan gum is warming up to 75 DEG C with revolving speed as 300r/min stirring
15min is uniformly mixed, and obtains water phase material；
(2) by 2 parts by weight glycerol monostearate, 2 parts by weight sorbitan monopalmitates, 2.5 parts by weight shea butters
It is added in the oily phase pot of vacuum homogeneous emulsifying machine, is heated to 75 DEG C, be that 300r/min stirring 15min is uniformly mixed with revolving speed, obtain
Mutually expect to oily；
(3) water phase material is pumped into the emulsion pot of vacuum homogeneous emulsifying machine, is pumped into oily phase in the case where 4500r/min stirs homogeneous
Material, homogeneous 8 minutes, then keep the temperature 15min at 75 DEG C, and 10 parts by weight skins containing human stem cell factor are added after being down to 25 DEG C of room temperature
Skin remediation composition is that 300r/min stirs the uniformly mixed skin care item to obtain the final product of 20min with revolving speed.
Performance of keeping humidity test: being tested using keratoderma hydration rate, and instrument used is CorneometerCM825, is led to
It crosses measurement and measures the variation of water content of stratum corneum using the variation of keratoderma capacitance before and after product, to be protected to evaluate
Wet effect.Test method: being randomly divided into 8 groups for 160 female subjects, every group 20, the age: 25-30 years old 10,30-35
Year 10, it is divided into experimental group 1-7 group and 1 group of control group, experimental group 1-7 group marks 3 in subject or so forearm side in the wrong respectively ×
3cm2The square Experimental Area of size, using left arm as the test zone of skin care item, uniform smearing embodiment every square centimeter
Skin care item 0.1g made of 1-7 human stem cell factor skin repair composition, the corresponding symmetrical region of right arm is blank control, right
Pure water 0.1g is smeared according to group, then with experiment position after Corneometer CM825 test detection smearing 8 hours of skin care item
Moisture content, be repeated 5 times respectively, obtain average value, calculated hydration rate.Hydration rate (%)=(test value-blank value)/blank
Value × 100.
Specific test result is shown in Table 2.Each group is compared with the control group the result shows that P < 0.05, difference have statistical significance.
Table 2: performance of keeping humidity test result table
| Hydration rate, % | |
| Embodiment 1 | 66 |
| Embodiment 2 | 71 |
| Embodiment 3 | 75 |
| Embodiment 4 | 68 |
| Embodiment 5 | 71 |
| Embodiment 6 | 73 |
| Embodiment 7 | 81 |
| Control group | 57 |
Porcine HGF is the optimum activity ingredient of skin nursing in the present invention, can be hindered through skin surface cell
And absorbed by deep skin cell, primordial stem cell effectively activating basal layer, no longer breaking up, enhance point of Skin Cell
Split proliferative capacity and metabolic function；It is safe, non-stimulated simultaneously containing bird's nest, the careless essence of resurrection, have both moisturizing, whitening is dispelled
Spot, crease-resistant effect, the mild safety of raw material of the present invention, do not damage skin, manufactured skin care item have excellent anti-oxidant, moisturizing,
Penetrating power effectively removes the free radical in skin, can infiltrate into deep skin, plays reparation, anti-oxidant, benefit from deep layer cells
The effect of water water lock.
Claims (10)
1. a kind of human stem cell factor skin repair composition, which is characterized in that by the raw material preparation of following mass percents
At: careless extract 40-50% human stem cell growth 0.00001-0.00002%, is brought back to life, surplus is birds nest extract.
2. human stem cell factor skin repair composition according to claim 1, which is characterized in that the careless extract of the resurrection
The preparation method comprises the following steps: crushed 10-50 mesh for grass is brought back to life, be 75-85% ethanol water by smashed resurrections grass, volume fraction
Solution is 1:(10-20 in mass ratio) mixing after ultrasonic extraction 30-60min, filtering, be concentrated into water content be 5-10wt%, obtain
To the careless extract of resurrection.
3. human stem cell factor skin repair composition according to claim 1, which is characterized in that the careless extract of the resurrection
The preparation method comprises the following steps: by bring back to life grass crushed 10-50 mesh, by it is smashed bring back to life grass investment supercritical extraction unit extraction
Carbon dioxide supercritical extraction 1-4h is carried out in kettle, obtains supercritical extract；Resurrection grass, phenols after supercritical extract is resisted
Oxidant, volume fraction are that 75-85% ethanol water is 1:(0.002-0.009 in mass ratio): ultrasound after (10-20) mixing
30-60min is extracted, filtering, being concentrated into water content is 5-10wt%, obtains sonicated extract；Supercritical extract, ultrasound are mentioned
It obtains bringing back to life careless extract after taking object to mix.
4. human stem cell factor skin repair composition according to claim 3, which is characterized in that the ultrasonic extraction surpasses
Acoustical power is 200-500W, supersonic frequency 30-50kHz.
5. human stem cell factor skin repair composition according to claim 3, which is characterized in that the carbon dioxide is super to be faced
Boundary extraction extraction temperature be 30-40 DEG C, extracting pressure 25-35MPa, carbon dioxide flow 10-30kg/h, separation temperature
For 28-32 DEG C, separating pressure 6-9MPa.
6. human stem cell factor skin repair composition according to claim 3, which is characterized in that the phenol antioxidant
For at least one of Cranberry polyphenol derivatives, green peel of walnut phenol natural antioxidant.
7. human stem cell factor skin repair composition according to claim 1, which is characterized in that the birds nest extract
In mass ratio it is 1:(5-15 by smashed bird's nest, water the preparation method comprises the following steps: bird's nest crushed 40-100 mesh) it mixes to turn
Speed is that 100-300r/min stirs 2-4h, and being then added in high pressure homogenizer in pressure is that 40-60MPa carries out high-pressure homogeneous processing
2-3 times, the time per treatment is 5-10min, obtains homogenization liquid；Homogenization liquid is first used into 500-1000 mesh filter cloth mistake
Then filter uses the ultrafiltration membrane ultrafiltration of 1000-10000Da, obtains filtrate；By filtrate temperature is -35~-55 DEG C, vacuum degree is
10-50Pa vacuum freeze drying to water content is 5-10wt%, obtains birds nest extract.
8. human stem cell factor skin repair composition according to claim 1, which is characterized in that the birds nest extract
In mass ratio it is 1 by smashed bird's nest, modification of chitosan solution the preparation method comprises the following steps: bird's nest crushed 40-100 mesh:
(5-15) mixing with revolving speed be 100-300r/min stir 2-4h, then be added high pressure homogenizer in pressure be 40-60MPa into
Horizontal high voltage homogenization 2-3 times, time per treatment are 5-10min, obtain homogenization liquid；Homogenization liquid is first used into 500-
Then 1000 mesh filter-cloth filterings use the ultrafiltration membrane ultrafiltration of 1000-10000Da, obtain filtrate；By filtrate temperature be -35~-55
DEG C, vacuum degree be that 10-50Pa vacuum freeze drying to water content is 5-10wt%, obtain birds nest extract.
9. human stem cell factor skin repair composition according to claim 8, which is characterized in that the modification of chitosan is molten
The preparation method of liquid is: acetic acid being added to the water to the acetum that 0.2-1.5wt% is made, chitosan, acetum are pressed matter
Amount is than being 1:(480-520) it is uniformly mixed, add cellulase, the acetum weight of acetum weight 0.1-0.5%
0.01-0.05% sodium citrate be 50-60 DEG C in temperature at revolving speed be 50-150r/min stirring 2-5h, then at 85-90 DEG C
It is living to keep the temperature 5-10min enzyme deactivation, obtains modification of chitosan solution.
10. application of the human stem cell factor skin repair composition in skin care item described in any one of claim 1-9.
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| CN112516042A (en) * | 2019-09-19 | 2021-03-19 | 孙忠旭 | Crystal penetrating eye cream |
| CN114762698A (en) * | 2021-06-16 | 2022-07-19 | 艾琳娜医疗管理(南京)有限公司 | Nutrient solution for stimulating collagen repair of female face and equipment method thereof |
| CN115308345A (en) * | 2022-08-26 | 2022-11-08 | 广东泰升药业有限公司 | Bird's nest ultra-high performance liquid chromatography construction method and detection method |
| CN116270392A (en) * | 2023-05-12 | 2023-06-23 | 宁波荣益生物科技有限公司 | Composition with moisturizing effect and application thereof |
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2019
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516042A (en) * | 2019-09-19 | 2021-03-19 | 孙忠旭 | Crystal penetrating eye cream |
| CN114762698A (en) * | 2021-06-16 | 2022-07-19 | 艾琳娜医疗管理(南京)有限公司 | Nutrient solution for stimulating collagen repair of female face and equipment method thereof |
| CN115308345A (en) * | 2022-08-26 | 2022-11-08 | 广东泰升药业有限公司 | Bird's nest ultra-high performance liquid chromatography construction method and detection method |
| CN115308345B (en) * | 2022-08-26 | 2023-12-12 | 广东泰升药业有限公司 | Construction method and detection method of bird's nest ultra-high performance liquid spectrum |
| CN116270392A (en) * | 2023-05-12 | 2023-06-23 | 宁波荣益生物科技有限公司 | Composition with moisturizing effect and application thereof |
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Application publication date: 20190521 |