KR102032116B1 - Method for preparing tea fermented materials and cosmetic composition comprising the same - Google Patents

Abstract

It relates to a method for producing a tea fermented product and a cosmetic composition comprising the same. According to the cosmetic composition for skin improvement according to one aspect, the jurocha fermented product fermented with jurocha lactic acid bacteria can be used for skin wound healing, skin barrier reinforcement, or moisturizing.

Description

Method for preparing tea fermented materials and cosmetic composition comprising the same}

It relates to a method for producing a tea fermented product and a cosmetic composition comprising the same.

Historically, green tea has been cultivated for the first time in China and India, and has since spread to Southeast Asia such as Java, Ceylon, Myanmar, Thailand, and East Asia in Korea and Japan, mainly in Asia below 35 ° C. Has been. Green tea has the scientific name Camellia sinensis L. It is a perennial seed plant belonging to the tea tree family, and it has a natural fragrance, natural color, natural taste, antibacterial action, antioxidant activity, anti-cancer activity and antiallergic action. It is known to have pharmacological properties in the human body, such as blood pressure lowering, antidiabetic, diuretic and detoxifying effects, and is one of the oldest consumed foods in the world. Green tea has been used as a cure for various diseases through its long history, cultivation and manufacturing techniques have been developed, and as green tea has been reported to maintain health and suppress the occurrence of diseases such as cancer, interest in green tea has increased. . Green tea is composed of various ingredients and contains various ingredients such as caffeine, tannin, chlorophyll, vitamin, and catechin.

Green tea catechin is a natural antioxidant known to have various physiological activities such as anti-inflammatory, anti-allergic and anti-cancer. Recently, tea has been used as an additive, such as food, medicine, health food, cosmetics by simply extracting functional ingredients from tea leaves as well as drinking beverages. Tea extracts for use in such applications have been used for extracting or drying hot tea after processing the green leaves of tea by thickening or sesame food process, but the process is complicated and consumes a lot of energy by going through several processing steps. It is pointed out that there is a large cost increase factor.

Therefore, to obtain high quality extract with improved extraction efficiency of green tea, we tried to prepare tea extract of new behavior by enzymatic treatment, and it was confirmed that this manufacturing method contains a large amount of green tea fermented products. It was confirmed that the improvement, skin barrier strengthening, and moisturizing effect is excellent, and completed the present invention.

One aspect is to provide a cosmetic composition for skin improvement comprising the jurocha fermented product fermented Jurocha with lactic acid bacteria as an active ingredient.

Another aspect is to provide a method of preparing jurocha fermentation.

One aspect provides a cosmetic composition for skin improvement comprising the jurocha fermented product fermented Jurocha with lactic acid bacteria as an active ingredient.

The skin improvement may be skin wrinkle improvement, moisturizing, whitening, anti-inflammatory, wound healing, or skin barrier strengthening. Jukcha tea fermented with jujube tea lactobacillus is excellent in skin wrinkle improvement effect, moisturizing effect, wound healing effect, or skin barrier strengthening effect compared to jurocha extract not fermented, the composition comprising the jukcha tea fermentation improves skin wrinkles It can be used as a composition for moisturizing, wound healing or skin barrier strengthening.

The term "improve skin wrinkles" may mean any action of inhibiting the expression of factors associated with wrinkles to prevent or improve wrinkles, or to inhibit collagenase activity to increase the total amount of collagen.

The term “skin moisturizing” can mean any action that keeps skin hydrated or prevents water loss.

The term “anti-inflammatory” may be used interchangeably with “inhibiting or improving inflammation” and may mean any action in which an immune response is alleviated to inhibit NO production.

The term “wound healing” may mean healing and / or regeneration of damaged tissue resulting from a wound. The wound healing may include the meaning of skin regeneration. In addition, the healing may be to maintain the original composition of the damaged tissue. In addition, the healing may be to promote healing and / or regeneration of the damaged tissue while minimizing complications and / or scarring of diseases associated with the wound.

The term "skin barrier strengthening" can mean any action that enhances impaired skin barrier function.

The term “improvement” may mean any action that at least reduces the degree of remission of a condition or a parameter associated with the treatment, eg, the degree of symptoms.

The "jukro tea" is a kind of tea, and refers to a tea made of tea leaves grown on bamboo dew. Tea plantation in Hwagae-myeon, Hadong-gun, Gyeongsangnam-do. Produced in fields. Bamboo tea is made between bamboos in the bamboo forest, so half-shape is created, and the half-shape naturally forms a warm and humid environment for the growth of tea trees, and the tea's bitterness is reduced. In one embodiment of the present invention, the juro tea may be a juro tea produced in the wave area.

In the composition according to one embodiment, the bamboo tea is completely dried by sun drying; Exposing the dried bamboo tea at night where dew is formed to naturally fermentate; And it may be a bamboo tea produced by the step of sterilizing the naturally fermented bamboo tea at high temperature. The nighttime exposure may be for about a week, and the sterilization treatment at high temperature may be for about 3 minutes to about 10 minutes at about 90 to 110 ° C., but is not limited thereto. Can be carried out in a

The "lactic acid bacteria" can be included without limitation, if the genus Lactobacillus. Specifically, Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus ashdophyllus ( Lactobacillus ) , Lactobacillus helveticus and Lactobacillus delbrueckii subspecies bulgaricus . The lactic acid bacteria may be isolated from nature, may be pre-sale but is not limited thereto.

In the composition according to one embodiment, the lactic acid bacteria may be vegetable lactic acid bacteria. In the present invention, 'vegetable lactic acid bacteria' may refer to lactic acid bacteria which is a raw material from which lactic acid bacteria are separated. Specifically, the lactic acid bacteria may be Lactobacillus rhamnosus .

The jurocha fermentation is obtained by fermenting jurocha at about 30 ° C to about 40 ° C, about 32 ° C to about 39 ° C, about 33 ° C to about 38 ° C, about 34 ° C to about 37.5 ° C, or 35 ° C to about 37 ° C. It may be.

The juro tea fermentation is obtained by fermenting juro tea for about 25 days to about 35 days, about 26 days to about 34 days, about 27 days to about 33 days, about 28 days to about 32 days, or about 29 days to about 31 days. It may be.

The jurocha ferment may be fermented jurocha at about 90 rpm to about 150 rpm, about 100 rpm to about 140 rpm, or about 110 rpm to about 130 rpm. The fermentation conditions may be appropriately selected from the fermentation temperature, rpm, and time in order to increase the wound healing effect, moisturizing effect, skin barrier strengthening effect through fermentation of jurocha.

For example, the jurocha fermentation may be fermented with lactic acid bacteria under the conditions of 100 rpm to 150 rpm for 35 days to 35 days at 35 ℃ to 37 ℃.

The fermentation may be carried out by inoculating the culture medium in which the lactic acid bacteria were cultured.

Culture medium of the lactic acid bacteria may be MRS (Man Rogosa Sharpe), lactose, YM (Yeast and Mold), M17 or ART (Asparagine Enrichment Broth) medium, but is not limited thereto.

The lactic acid bacteria can be inoculated with about 0.1 × 10 ⁸ to about 1 × 10 ⁹ cells, about 0.5 × 10 ⁸ to about 2 × 10 ⁸ cells, or about 1 × 10 ⁸ to about 5 × 10 ⁸ cells for about 1 kg of Jurocha. Can be.

The inoculation conditions may be appropriately selected from the medium, strain number used at the time of inoculation, in order to increase the wound healing effect, moisturizing effect, skin barrier strengthening effect through fermentation of Jurocha.

For example, the lactic acid bacteria may be inoculated with about 1 × 10 ⁸ to about 5 × 10 ⁸ cells in MRS (Man Rogosa Sharpe) medium.

In the composition according to one embodiment, the jurocha fermentation may be further fermented by low temperature aging after fermenting jurocha with lactic acid bacteria.

The low temperature aging may be to immerse the jade in the jurocha fermentation and to mature at about 2 ℃ to about 7 ℃, or about 3 ℃ to about 6 ℃.

The low temperature aging may be immersed in the jukcha tea fermentation and further fermented for about 65 days to about 75 days, about 67 days to about 73 days, or about 68 days to about 72 days.

For example, the low temperature aging may be immersed in jade tea lactic acid bacteria and then immersed in jade and further fermented for 65 to 75 days at 2 ℃ to 7 ℃.

The composition according to one embodiment may have a wound healing effect, a skin barrier strengthening effect, or a moisturizing effect.

The composition according to one embodiment may be to increase the expression of claudin 1, filaggrin, or hyaluronic acid synthase 3 (HAS3).

The composition according to one embodiment may exhibit a wound healing effect, skin barrier strengthening effect, or moisturizing effect by increasing the expression of claudin 1, filaggrin, or hyaluronic acid synthase 3 (HAS3). .

The composition according to one embodiment may comprise the jurocha fermentation as an effective amount, or as an active ingredient. The effective amount may be appropriately selected depending on the individual. Severity of the disease or condition, age, weight, health, sex of the subject, sensitivity to the extract of the subject, time of administration, route of administration and rate of excretion, duration of administration, other elements, including other compositions in combination or concurrently with the composition It may be determined according to factors well known in the physiological or medical field.

For example, the jurocha fermentation may be included in about 0.001% to about 10% by weight based on the total weight of the cosmetic composition, but is not limited thereto.

Cosmetic composition for skin improvement according to one embodiment may further comprise a cosmetically acceptable excipient or carrier. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be sodium starch glycolate, calcium dihydrogen phosphate anhydride, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

The composition may be formulated in a parenteral dosage form. The parenteral dosage form may be an injection or an external dermal preparation. The external dermal agent may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug containing bandage, lotion, or a combination thereof.

The external skin preparations are commonly used in external preparations for cosmetics and medicines, such as aqueous components, oily ingredients, powder ingredients, alcohols, humectants, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , Colorants, and various skin nutrients can be appropriately blended as needed.

The external skin preparations include metal blockers such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belafamil, licorice extract, glablidine, and caline. Fruit hot water extract, various herbal medicines, drugs such as tocopherol acetate, glytilinic acid, tranexamic acid and derivatives thereof or salts thereof, vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix | blended suitably.

The composition according to one embodiment includes a lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, essence, nourishing essence , Packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, suspensions, gels, powders, pastes, mask packs or sheets or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art. The cosmetic composition may further include preservatives, stabilizers, surfactants, solubilizers, moisturizers, emollients, ultraviolet absorbers, preservatives, fungicides, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, coolants or limiters, etc. have. The blending amount of the additional components such as the moisturizing agent can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount is about 0.001 to about 10% by weight, specifically about 0.01 to about 3 weight percent.

Another aspect provides a method of ameliorating a skin of a subject comprising applying the composition to the subject. The composition is as described above.

Specifically, the method may be a method of strengthening an individual's skin barrier, a method of maintaining skin moisture or effectively preventing moisture loss for moisturizing an individual's skin, or a method of improving wound healing of an individual.

The terms “applying”, “administering”, “introducing” and “transplanting” are used interchangeably and result in an individual by a method or route that results in at least partial localization of the composition to the desired site according to one embodiment. It can mean the placement of a composition according to one embodiment into it. The fermentation or fermentation component of the composition according to one embodiment may be administered by any suitable route for delivering to a desired location in the surviving individual.

Administration can be by any method known in the art. Administration can be administered directly to the subject by any means, for example, by intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. Can be. The administration can be administered systemically or locally.

The subject may be a mammal, for example a human, a cow, a horse, a pig, a dog, a sheep, a goat, or a cat. The subject may be a subject in need of skin condition improvement, for example skin wrinkle improvement, moisturizing, skin barrier strengthening, or skin wound healing.

The dosage of the Jurocha ferment may be variously prescribed by such factors as the formulation method, mode of administration, age, weight, sex, pathological condition, food, time of administration, route of administration, rate of excretion and response to the patient, Those skilled in the art can consider these factors and adjust the dosage appropriately. The number of administrations may be once or twice a day within the range of clinically acceptable side effects, and may be administered in one or two or more places on the administration site, and may be administered daily or every two to five days. Dosing days can be administered from 1 to 30 days once treated. If necessary, the same treatment may be repeated after the titration period. In the case of animals other than humans, the same dosages as humans per kg or the above dosages are converted into volume ratios (for example, average values) of organs (heart, etc.) between the target animal and humans. One dose may be administered.

Another aspect is a step of fermenting jurocha with lactic acid bacteria to prepare a primary fermentation product; And immersing jade in the primary fermentation product and then aging at low temperature to prepare a secondary fermentation product.

The juro tea, lactic acid bacteria, fermented with lactic acid bacteria, immersed in jade, low temperature aging, and jurocha fermented products are as described above.

In the method according to one embodiment, the lactic acid bacteria may be included without limitation if the genus Lactobacillus. Specifically, Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus ashdophyllus ( Lactobacillus ) , Lactobacillus helveticus and Lactobacillus delbrueckii subspecies bulgaricus . The lactic acid bacteria may be isolated from nature, may be pre-sale but is not limited thereto.

The lactic acid bacteria may be vegetable lactic acid bacteria. In the present invention, 'vegetable lactic acid bacteria' may refer to lactic acid bacteria which is a raw material from which lactic acid bacteria are separated. Specifically, the lactic acid bacteria may be Lactobacillus rhamnosus .

In a method according to one embodiment, the fermentation in the step of preparing the primary fermentation may be performed at a condition of 100 rpm to 150 rpm for 25 days to 35 days at 35 ℃ to 37 ℃.

In the method according to one embodiment, in the step of preparing the secondary fermentation may be one that is carried out at 2 ℃ to 7 ℃ for 65 days to 75 days.

In a method according to one embodiment, the method may further comprise centrifuging the secondary fermentation, taking a supernatant, or filtering. The centrifugation, taking off the supernatant, or filtration can be carried out by general methods known in the art.

The jurocha ferment prepared by the method can increase the expression of claudin 1, filaggrin, or hyaluronic acid synthase 3 (HAS3) to heal wounds of the skin, strengthen the skin barrier, , Excellent moisturizing effect. The skin barrier reinforcing effect, moisturizing effect, or skin wound healing effect of these jurocha fermentation was significantly increased compared to the jurocha which was not fermented. Therefore, the jurocha fermented product prepared by the above method can be utilized for various purposes such as cosmetics, food, pharmaceutical compositions.

According to the cosmetic composition for skin improvement according to one aspect, the jurocha fermented product fermented with jurocha lactic acid bacteria can be used for skin wound healing, skin barrier reinforcement, or moisturizing.

1 shows the results of relative levels of claudin 1 mRNA. Control group: HaCaT cells treated with nothing; Birch sap treatment group: HaCaT cells treated with birch sap; Jurocha fermentation raw material treatment group: HaCaT cells treated with jurocha before fermentation; Jurocha primary fermentation treatment group: HaCaT cells treated with Jurocha primary fermentation product prepared in Example 2; Jurocha fermentation treatment group: HaCaT cells treated with jurocha fermentation of the present invention.
Figure 2 is a result showing the relative levels of filagrin (filagrin) mRNA. Control group: HaCaT cells treated with nothing; Birch sap treatment group: HaCaT cells treated with birch sap; Jurocha fermentation raw material treatment group: HaCaT cells treated with jurocha before fermentation; Jurocha primary fermentation treatment group: HaCaT cells treated with Jurocha primary fermentation product prepared in Example 2; Jurocha fermentation treatment group: HaCaT cells treated with jurocha fermentation of the present invention.
3 shows the results of relative levels of HAS3 mRNA. Control group: HaCaT cells treated with nothing; Birch sap treatment group: HaCaT cells treated with birch sap; Jurocha fermentation raw material treatment group: HaCaT cells treated with jurocha before fermentation; Jurocha primary fermentation treatment group: HaCaT cells treated with Jurocha primary fermentation product prepared in Example 2; Jurocha fermentation treatment group: HaCaT cells treated with jurocha fermentation of the present invention.

It will be described in more detail through the following examples. However, these examples are provided to illustrate one or more embodiments illustratively and the scope of the present invention is not limited to these examples.

Example  1. Preparation of Juro Tea and Juro Tea Fermentation Raw Materials

Juro tea was prepared by crushing after harvesting at the Hongsosul Jukro tea farm in Hadong region. The dried bamboo tea was sun-dried and the fully dried tea was exposed to the outdoors at night when dew formed, allowing natural fermentation to proceed for about 1 week. The naturally fermented tea was collected and mixed with 1000 g of birch sap (Incheon, Gangwon-do), and 5 g of the collected juro tea. The mixture was placed in a fermenter and sterilized at about 100 ° C. for about 5 minutes, and then the temperature was lowered to about 37 ° C. to prepare fermentation raw materials.

Example  2. Primary Fermentation of Jurocha Fermentation Raw Materials Using Lactic Acid Bacteria

The juro tea fermentation raw material prepared in Example 1 was fermented with lactic acid bacteria.

Specifically, Lactobacillus rhamnosus (KFCC11028) was directly isolated as lactic acid bacteria. The lactic acid bacteria were cultured in liquid phase in MRS (Man Rogosa Sharpe: BD) culture medium to obtain a culture solution. 200 ml of the lactic acid bacteria culture solution was inoculated so that the lactic acid bacteria per 1 kg of the fermented raw material prepared in Example 1 was contained in about 1 × 10 ⁸ to about 5 × 10 ⁸ cells. After inoculation, fermentation was carried out for about 30 days under fermentation conditions of about 35 to about 37 ° C, pH 7, and agitation speed of about 100-120 rpm.

The fermented product obtained above was centrifuged and the precipitate and the cells were removed to obtain a fermentation broth. 900 g of distilled water was added to the fermentation broth, which was placed in an extractor equipped with a cooling condenser and heated to about 80 ° C. for about 6 hours, and then 800 L of distilled liquor was recovered. The fermentation product obtained above was first filtered with 300 mesh filter paper and left at room temperature for 1 week, and then filtered secondly with 150 mm filter paper to prepare a primary fermentation product.

Example  3. Jurocha Fermented product  Secondary fermentation

The first fermented product prepared in Example 2 was aged at low temperature for secondary fermentation.

Specifically, the fermentation product was recovered after the first fermentation was stopped and the autoclaved jade was immersed at about 100 ° C. for about 5 minutes. At this time, the fermented product and jade were moved to Onggi, and the onggi was kept for about 70 days in a constant temperature refrigerator maintained at a low temperature of about 4 ℃ to proceed to low temperature to prepare a secondary fermentation.

Example  4. Jurocha Fermented product  Produce

The secondary fermentation product prepared in Example 3 was centrifuged at about 14000 rpm for about 1 hour to remove precipitates and cells as a by-product of fermentation, and only fermentation broth was recovered. The supernatant was taken from the fermentation product obtained through centrifugation, filtered first with a 0.2 μm filter, left at room temperature for about one week, and then filtered through a 0.2 μm filter to prepare jurocha ferment.

Experimental Example  1. Jurocha Fermented product  Evaluation of skin wound healing effect, skin barrier strengthening effect, moisturizing effect

An experiment was conducted to evaluate the skin wound healing effect, skin barrier strengthening effect, and moisturizing effect of the jurocha fermentation prepared in Example 4.

Specifically, HaCaT cells, which are human keratinocytes, were cultured in DMEM medium (Dulbecco'smodified Eagle's Medium, Gibco 1210-0038) containing 10% fetal bovin serum, and the cultures were all 37 ° C., It was performed in a 5% CO ₂ incubator. As a positive control to the cultured cell lines, retinol was treated at a 1 mM dose. Jurocha ferment was treated at a concentration of 1.0%.

Thereafter, the HaCaT cells were further cultured for 24 hours, and then recovered and 1 ml of trizol (RNA iso, DAKARA, Japan) was added to separate RNA. In addition, for RT-PCR, RNA was quantified using Nanodrop 2000 (Thermo, USA), and then cDNA was synthesized by reacting for 55 minutes at 42 ° C and 15 minutes at 70 ° C (Reverse Transcriptase Mix, ELPIS biotech, Korea ).

RT-PCR was confirmed by RT-PCR expression of filaggrin, HAS3 (hyaluronic acid synthase3), and clauadin 1 genes, which are factors related to skin wound healing, skin barrier strengthening, and moisturizing. Step One Plus (Applied Biosystems, USA) was used, and Cyberline (SYBR Green supermix, Applied Biosystems, USA) was added with primers and cDNA to activate the polymerase at 94 ° C. for 5 minutes, followed by 95 ° C. 30 seconds. The polymerization was carried out in 40 cycles of 1 minute at 54 ° C and 1 minute at 72 ° C. The primers used are shown in Table 1 below. After completion of the polymerization, the expression levels of the Cladin 1, Filaggrin, and HAS3 genes were normalized to the mRNA expression levels of β-actin to calculate relative expression levels.

gene SEQ ID NO: primer  designation primer  direction primer  order Claudin 1 One Claudin 1-f Forward direction 5'-GCTCTAGAATTCTCACACGTAGTCTTTCCCGCT-3 ' 2 Claudin 1-r Reverse 5'-GCTCTAGAATTCTCACACGTAGTCTTTCCCGCT-3 ' filagrin 3 filagrin -F Forward direction 5'-AGTGCACTCAGGGGGCTCACA-3 ' 4 filagrin -R Reverse 5'-CCGGCTTGGCCGTAATGTGT-3 ' HAS3 5 HAS3-F Forward direction 5'-CTTAAGGGTTGCTTGCTTGC-3 ' 6 HAS3-R Reverse 5'-GTTCGTGGGAGATGAAGGAA-3 ' β-Actin 7 Beta actin-F Forward direction 5'-AGAGCTACGAGCTGCCTGAC-3 ' 8 Beta actin-R Reverse 5'-AGCACTGTGTTGGCGTACAG-3 '

1 shows the results of relative levels of cloudin 1 mRNA. The first bar from the left is HaCaT cells (control) without any treatment, and the second to fourth bars are HaCaT cells (birch sap treated group), each treated with birch sap; HaCaT cells treated with jurocha before fermentation (jurocha fermentation raw material treatment group); HaCaT cells treated with jurocha primary fermentation product prepared in Example 2 (jurocha primary fermentation treatment group); And the relative mRNA levels of Claudin 1 in HaCaT cells treated with Jurocha fermentation of the present invention (jurocha fermentation treatment group).

2 shows the results of relative levels of filaggrin mRNA. The first bar from the left is HaCaT cells (control) without any treatment, and the second to fourth bars are HaCaT cells (birch sap treated group), each treated with birch sap; HaCaT cells treated with jurocha before fermentation (jurocha fermentation raw material treatment group); HaCaT cells treated with Jurocha primary fermentation product prepared in Example 2 (jurocha primary fermentation treatment group); And relative mRNA levels of filaggrins in HaCaT cells treated with Jurocha fermentation of the present invention (jurocha fermentation treatment group).

3 shows the results of relative levels of HAS3 mRNA. The first bar from the left is HaCaT cells (control) without any treatment, and the second to fourth bars are HaCaT cells (birch sap treated group), each treated with birch sap; HaCaT cells treated with jurocha before fermentation (jurocha fermentation raw material treatment group); HaCaT cells treated with jurocha primary fermentation product prepared in Example 2 (jurocha primary fermentation treatment group); And relative mRNA levels of HAS3 in HaCaT cells treated with Jurocha fermentation of the present invention (jurocha fermentation treatment group).

As shown in Figures 1 to 3, when treated with Jurocha fermented raw material or Jurocha primary fermentation product to human keratinocyte cell line, Cladin 1, filaggrin, and HAS3 genes that are factors related to skin wound healing, skin barrier strengthening and moisturizing It was confirmed that the relative mRNA expression level was similar or lower than that of the control birch sap. On the other hand, the jurocha fermentation product of the present invention shows a significantly higher mRNA expression compared to the case of treating other substances, it can be seen that the skin wound healing effect, skin barrier strengthening effect and skin moisturizing effect is very excellent.

<110> Cosmax Co., Ltd. <120> Method for preparing tea fermented materials and cosmetic          composition comprising the same <130> PN120339 <160> 8 <170> KoPatentIn 3.0 <210> 1 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Claudin 1-F primer <400> 1 gctctagaat tctcacacgt agtctttccc gct 33 <210> 2 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Claudin 1-R primer <400> 2 gctctagaat tctcacacgt agtctttccc gct 33 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> filagrin-F primer <400> 3 agtgcactca gggggctcac a 21 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> filagrin-R primer <400> 4 ccggcttggc cgtaatgtgt 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS3-F primer <400> 5 cttaagggtt gcttgcttgc 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS3-R primer <400> 6 gttcgtggga gatgaaggaa 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Beta actin-F primer <400> 7 agagctacga gctgcctgac 20 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Beta actin-R primer <400> 8 agcactgtgt tggcgtacag 20

Claims (11)

After immersing jade in the fermented product of jurocha fermented with lactic acid bacteria, after aging at low temperature, the cosmetic composition for skin improvement comprising the jurocha fermented product further fermented. The method according to claim 1, wherein the lactic acid bacteria Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus casi ( Lactobacillus casei ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus ashdophyllus Lactobacillus acidophilus , Lactobacillus helveticus and Lactobacillus delbrueckii subspecies bulgaricus Cosmetic composition is selected from the group consisting of. The cosmetic composition of claim 1, wherein the fermentation product of fermenting the juro tea with lactic acid bacteria is fermented with lactic acid bacteria under conditions of 100 rpm to 150 rpm for 25 days to 35 days at 35 ° C to 37 ° C. The cosmetic composition according to claim 1, wherein the additionally fermented jurocha fermentation is immersed in jade after fermenting the jurocha with lactic acid bacteria and further fermented for 65 to 75 days at 2 ℃ to 7 ℃. The cosmetic composition of claim 1, wherein the jurocha fermented product is included in an amount of 0.001% to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, which has a wound improvement effect, a skin barrier strengthening effect, or a moisturizing effect. The cosmetic composition of claim 1, wherein the cosmetic composition increases the expression of claudin 1, filaggrin, or hyaluronic acid synthase 3 (HAS3). Fermenting jurocha with lactic acid bacteria to prepare a primary fermentation product; And
After immersing the jade in the primary fermentation and aging at low temperature to prepare a jurocha fermentation comprising the step of preparing a secondary fermentation. The method according to claim 8, wherein the lactic acid bacteria Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus brevis ( Lactobacillus brevis ), Lactobacillus casi ( Lactobacillus casei ), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus ashdophyllus Lactobacillus acidophilus , Lactobacillus helveticus and Lactobacillus delbrueckii subspecies bulgaricus . The method of claim 8, wherein the fermentation in the step of preparing the primary fermentation is performed at a condition of 100 rpm to 150 rpm for 25 days to 35 days at 35 ° C. to 37 ° C. 10. The method according to claim 8, wherein in the step of preparing the secondary fermentation is carried out for 65 to 75 days at 2 ℃ to 7 ℃.

Images