KR20190062034A - Method for preparing tea fermented materials and cosmetic composition comprising the same - Google Patents

Abstract

The present invention relates to a method of preparing fermented tea products and a cosmetic composition comprising the same. According to one embodiment, a cosmetic composition for improving the skin comprises a fermentation product of bamboo dew tea, obtained by fermenting bamboo dew tea with lactic acid bacteria, as an effective component, and may be used for healing scars on the skin, strengthening skin barriers, or moisturizing the skin.

Description

FIELD OF THE INVENTION The present invention relates to a method for preparing fermented tea and a cosmetic composition containing the fermented tea fermented material,

And a cosmetic composition containing the same.

Historically, green tea has been cultivated for the first time in China and India, and has since been spread to Southeast Asian regions such as Java, Ceylon, Myanmar, and Thailand and East Asia in Korea and Japan. Has come. Green tea is recorded as Camellia sinensis L. and is a perennial seed plant belonging to the genus Camellia sinensis L. It is a natural seed, natural color, natural flavor and antimicrobial activity, antioxidant activity, anti-cancer effect, antiallergic activity , Blood pressure lowering effect, anti-diabetic effect, diuretic and detoxifying action, and it is one of the oldest consuming foods in the world. Green tea has long been used as a treatment for various diseases, has been developed and cultivated, and green tea has been reported to maintain health and inhibit the development of diseases such as cancer, thereby increasing interest in green tea . Green tea is composed of various ingredients and contains a variety of ingredients such as caffeine, tannin, chlorophyll, vitamins and catechins.

Green tea catechin is a natural antioxidant and is known to have various physiological activities such as anti-inflammation, antiallergic and anti-cancer. Recently, tea is used not only as a drink but also as an additive for foods, medicines, health foods, cosmetics and the like by extracting functional ingredients from tea leaves. Tea extracts for use in such applications have been prepared by extruding fresh leaves of a tea into a dried or dried tea and then extracting it with hot water. However, the process is complicated and requires a lot of energy Which is a major cause of cost increase.

Therefore, in order to obtain a high-quality extract having improved extraction efficiency of the functional ingredient of green tea, an enzyme treatment is performed to produce a tea extract of a new behavior, and it has been confirmed that this production method contains a large amount of functional ingredient, Skin barrier enhancement, and moisturizing effect, and completed the present invention.

One aspect of the present invention is to provide a cosmetic composition for improving skin comprising, as an active ingredient, a fermented killed body fermented with lactic acid bacteria.

Another aspect is to provide a method for producing a killed fermented product.

One aspect of the present invention is to provide a cosmetic composition for skin improvement comprising a fermented killed body fermented with lactic acid bacteria as an active ingredient.

The skin improvement may be skin wrinkle improvement, moisturizing, whitening, anti-inflammation, wound healing, or skin barrier enhancement. The fermented jujube fermented by fermenting the fermented milk with lactic acid bacterium has superior wrinkle improving effect, moisturizing effect, wound healing effect, or skin barrier strengthening effect compared with unfermented fermented tea extract, , Moisturizing, wound healing or skin barrier strengthening.

The term " improvement of skin wrinkles " may mean all actions that inhibit the expression of factors related to wrinkles to prevent or improve wrinkles, or inhibit collagenase activity and thereby increase the total amount of collagen.

The term " skin moisturizing " may mean any action that maintains skin moisture or prevents moisture loss.

The term " anti-inflammatory " can be used interchangeably with " inflammation inhibition or improvement ", and may mean all actions in which the immune response is alleviated and NO production is suppressed.

The term " wound healing " may mean healing and / or regeneration of damaged tissue resulting from a wound. The wound healing may include the meaning of skin regeneration. The healing may also be to maintain the original composition of the damaged tissue. In addition, the healing may be to promote healing and / or regeneration of the damaged tissue while minimizing complications and / or scarring of wound related diseases.

The term " skin barrier enhancement " may refer to any action that enhances an impaired skin barrier function.

The term " improvement " may mean all actions that at least reduce the degree of relief or the parameters associated with the treatment, for example, symptoms.

The "bamboo tea" is a type of tea, which refers to a tea made from tea leaf that has been bamboo dew. Gyeongsangnam-do Hyeong-gun, Hwacheon-myeon, Gyeongsangnam-do, Gyeongsangnam-do, Gyeongsang-myeon, Gyeongsang-myeon, Gyeongnam-myeon, It is produced in field and the like. Bamboo leaves are made from bamboo in bamboo forests, so that the bamboo leaves are made in a semi-shiny state. In one embodiment of the present invention, the dead body may be a dead body produced in a wave region.

In a composition according to an embodiment, said blanket is completely dried by daylight drying; Exposing the dried bamboo shoots to nighttime in which dew is formed, and naturally fermenting the bamboo leaves; And a fermentation product produced by sterilizing the naturally fermented fat burner at a high temperature. The nighttime exposure may be about one week of exposure and the sterilization treatment at the elevated temperature may be about 90 to 110 DEG C for about 3 minutes to about 10 minutes, . ≪ / RTI >

The above-mentioned " lactic acid bacteria " may include any strains of Lactobacillus. Specific examples thereof include Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus acidophilus , Lactobacillus spp . , Lactobacillus helveticus , and Lactobacillus delbrueckii subspecies bulgaricus. ≪ Desc / Clms Page number 2 > The lactic acid bacterium may be isolated from nature, and may be cultured, but is not limited thereto.

In a composition according to one embodiment, the lactic acid bacteria may be plant lactic acid bacteria. In the present invention, 'vegetable lactic acid bacterium' may refer to a lactic acid bacterium in which the raw material from which the lactic acid bacterium is isolated is a plant. Specifically, the lactic acid bacterium may be Lactobacillus rhamnosus .

Wherein the killed fermented product is fermented at about 30 ° C to about 40 ° C, about 32 ° C to about 39 ° C, about 33 ° C to about 38 ° C, about 34 ° C to about 37.5 ° C, or 35 ° C to about 37 ° C Lt; / RTI >

Wherein the killed fermented product is fermented for about 25 days to about 35 days, about 26 days to about 34 days, about 27 days to about 33 days, about 28 days to about 32 days, or about 29 days to about 31 days Lt; / RTI >

The killed fermented product may be fermented at a temperature ranging from about 90 rpm to about 150 rpm, from about 100 rpm to about 140 rpm, or from about 110 rpm to about 130 rpm. The fermentation conditions may be appropriately selected from fermentation temperature, rpm, and time to increase the wound healing effect, the moisturizing effect, and the skin barrier strengthening effect through fermentation of dead leaves.

For example, the killed fermented product may be fermented with lactic acid bacterium at a temperature ranging from 100 rpm to 150 rpm for 35 to 35 days at 35 ° C to 37 ° C.

The fermentation can be carried out by inoculating a killed culture medium with lactic acid bacteria.

The culture medium of the lactic acid bacteria may be MRS (Man Rogosa Sharpe), lactose, Yeast and Mold, M17 or ARP (Asparagine Enrichment Broth) medium, but is not limited thereto.

The lactic acid bacteria may be inoculated at about 0.1 x 10 ⁸ to about 1 x 10 ⁹ cells, about 0.5 x 10 ⁸ to about 2 x 10 ⁸ cells, or about 1 x 10 ⁸ to about 5 x 10 ⁸ cells, .

The inoculation condition can be appropriately selected from the medium and the number of strains used for inoculation in order to increase the wound healing effect, the moisturizing effect and the skin barrier strengthening effect through fermentation of dead leaves.

For example, the lactic acid bacteria may be inoculated at about 1 × 10 ⁸ to about 5 × 10 ⁸ cells in MRS (Man Rogosa Sharpe) medium.

In the composition according to one embodiment of the present invention, the killed fermented product may be fermented by lactic acid bacteria and then further fermented by low temperature aging.

The low temperature aging may be such that the jelly is immersed in the fermented fermented product and aged at about 2 [deg.] C to about 7 [deg.] C, or at about 3 [deg.] C to about 6 [deg.] C.

The low temperature aging may be further fermentation for about 65 days to about 75 days, about 67 days to about 73 days, or about 68 days to about 72 days by soaking jade in the fermented fermented product.

For example, the low-temperature aging may be performed by fermenting the fermented milk with lactic acid bacteria, then immersing the jade, and further fermenting the fermented milk at 2 ° C to 7 ° C for 65 to 75 days.

The composition according to one embodiment may be one having a wound healing effect, a skin barrier strengthening effect, or a moisturizing effect.

The composition according to one embodiment may be one that increases the expression of claudin 1, filagrin, or HAS3 (hyaluronic acid synthase 3).

The composition according to one embodiment may be one that exhibits a wound healing effect, a skin barrier enhancing effect, or a moisturizing effect by increasing the expression of claudin 1, filagrin, or hyaluronic acid synthase 3 (HAS3) .

The composition according to one embodiment may contain an effective amount or effective ingredient of the killed fermented product. The effective amount may be appropriately selected depending on the individual. The severity of the disease or condition, the age, weight, health, sex, sensitivity of the individual to the extract, time of administration, route and rate of excretion, duration of administration, factors including other compositions combined or co- May be determined according to factors well known in the art, physiology or medical field.

For example, the killed fermented product may be included in an amount of about 0.001% by weight to about 10% by weight based on the total weight of the cosmetic composition, but is not limited thereto.

The cosmetic composition for skin improvement according to one embodiment may further comprise cosmetically acceptable excipients or carriers. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low substituted hydroxy cellulose, or a combination thereof. The disintegrant may be sodium starch glycolate, calcium monohydrogen phosphate anhydrous, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or combinations thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

The composition may be formulated into a parenteral dosage form. The parenteral dosage form may be an injection or an external preparation for skin. The external skin preparation may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a drug-containing bandage, a lotion, or a combination thereof.

The external preparation for skin is usually used as a component used in external skin preparations such as cosmetics or medicines such as an aqueous component, an oily component, a powder component, an alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, an antiseptic, , Coloring agents, various skin nutrients, and the like can be appropriately blended as needed.

The external preparation for skin may be a metal blocker such as sodium edetate, sodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate or gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridine, Vitamin C, ascorbic acid magnesium phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, fructose, fructose and other herbal medicines, various herbal medicines, tocopherol acetate, glycyrrhizic acid, Sugars such as trehalose and the like can also be appropriately compounded.

The composition according to an exemplary embodiment may be at least one selected from the group consisting of a lotion (skin lotion), a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizer cream, a hand cream, , A pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, a suspension, a gel, a powder, a paste, a mask pack or a sheet or an aerosol composition. Compositions of such formulations may be prepared according to methods conventional in the art. The cosmetic composition may further contain preservatives, stabilizers, surfactants, solubilizers, moisturizers, emollients, ultraviolet absorbers, preservatives, bactericides, antioxidants, pH regulators, organic and inorganic pigments, fragrances, have. The amount of the additional component such as the above-mentioned moisturizing agent can be easily selected by a person skilled in the art within a range not to impair the purpose and effect of the present invention, and the amount thereof is about 0.001 to about 10% by weight, 0.01 to about 3% by weight.

Another aspect provides a method of improving the skin of an individual comprising applying the composition to an individual. The above composition is as described above.

Specifically, the method may be a method of enhancing skin barrier of an individual, a method of efficiently maintaining skin moisture or water loss for skin moisturization of an individual, and a method of improving wound healing of an individual.

The terms " applying, " " administering, " " introducing " and " transplanting " are used interchangeably and refer to a method or route by which a composition according to one embodiment results in at least partial localization to a desired site May refer to the placement of the composition according to one embodiment into the interior. May be administered by any suitable route of delivery of at least a portion of the fermentation product or fermentation component of the composition according to one embodiment to the desired location within the living individual.

Administration can be by any method known in the art. Administration may be by any means directly administered to a subject, such as by intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration, . The administration can be systemically or locally administered.

The subject may be a mammal, such as a person, a cow, a horse, a pig, a dog, a sheep, a goat, or a cat. The subject may be an individual in need of skin condition improvement, for example, skin wrinkle improvement, moisturizing, skin barrier enhancement, or skin wound healing.

The dosage of the killed fermented product can be variously regulated by such factors as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient, Those skilled in the art will appreciate that these factors may be taken into account to properly control the dosage. The number of doses may be at least once per day or within the range of clinically acceptable side effects and may be administered at one or two or more doses at the site of administration and may be administered daily or every two to five days The number of days of administration can be administered from one day to 30 days at one time of treatment. If necessary, the same treatment may be repeated after the appropriate time. For an animal other than a human, the dosage may be the same as that of a human per kg, or the dosage may be converted into a dose in terms of a volume ratio (for example, an average value) One dose may be administered.

Another aspect is a method for producing fermented milk, comprising the steps of: fermenting a dead fermented with a lactic acid bacterium to prepare a primary fermented product; And a step of immersing the primary fermentation product in jade and aging at a low temperature to produce a secondary fermentation product.

The fermentation with the above-mentioned dead, lactic acid bacteria, lactic acid bacteria, immersion of jade, fermentation at low temperatures, and fermentation of dead leaves are as described above.

In a method according to one embodiment, the lactic acid bacterium may include Lactobacillus spp. Without limitation. Specific examples thereof include Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus acidophilus , Lactobacillus spp . , Lactobacillus helveticus , and Lactobacillus delbrueckii subspecies bulgaricus. ≪ Desc / Clms Page number 2 > The lactic acid bacterium may be isolated from nature, and may be cultured, but is not limited thereto.

The lactic acid bacteria may be vegetable lactic acid bacteria. In the present invention, 'vegetable lactic acid bacterium' may refer to a lactic acid bacterium in which the raw material from which the lactic acid bacterium is isolated is a plant. Specifically, the lactic acid bacterium may be Lactobacillus rhamnosus .

In the method according to one embodiment, in the step of preparing the primary fermentation, the fermentation may be carried out at a temperature of 35 to 37 DEG C for 25 to 35 days at 100 rpm to 150 rpm.

In the method according to one embodiment, the aging in the step of preparing the secondary fermentation product may be performed at 2 ° C to 7 ° C for 65 days to 75 days.

In a method according to one embodiment, it may further comprise centrifuging the secondary fermentation, taking up a supernatant, or filtering. The centrifugation, supernatant, or filtration can be carried out by conventional methods known in the art.

The fermented fermented product produced by the above method can increase the expression of claudin 1, filagrin, or hyaluronic acid synthase 3 (HAS3) to heal skin wounds, strengthen the skin barrier , It can exhibit an excellent moisturizing effect. The skin barrier strengthening effect, moisturizing effect, or skin wound healing effect of the fermented product of the killed body was remarkably increased as compared with unfermented dead body. Thus, the fermented fermented product produced by the above method can be used for various purposes such as cosmetics, foods, pharmaceutical compositions, and the like.

According to a cosmetic composition for skin improvement according to one aspect, a fermented product of fermented fermented carrot fermented with lactic acid bacteria can be used for skin wound healing, skin barrier enhancement, or moisturizing.

Figure 1 shows the relative levels of claudin 1 mRNA. Control: HaCaT cells without any treatment; Birch sap treatment group: HaCaT cells treated with birch sap; Dead-root fermentation raw material treatment group: HaCaT cells treated with dead before fermentation; Treated primary fermented product: HaCaT cells treated with the primary fermented products prepared in Example 2; Treated group of Haeca fermented product: HaCaT cells treated with the killed fermented product of the present invention.
Figure 2 shows the relative levels of filagrin mRNA. Control: HaCaT cells without any treatment; Birch sap treatment group: HaCaT cells treated with birch sap; Dead-root fermentation raw material treatment group: HaCaT cells treated with dead before fermentation; Treated primary fermented product: HaCaT cells treated with the primary fermented products prepared in Example 2; Treated group of Haeca fermented product: HaCaT cells treated with the killed fermented product of the present invention.
Figure 3 shows the relative levels of HAS3 mRNA. Control: HaCaT cells without any treatment; Birch sap treatment group: HaCaT cells treated with birch sap; Dead-root fermentation raw material treatment group: HaCaT cells treated with dead before fermentation; Treated primary fermented product: HaCaT cells treated with the primary fermented products prepared in Example 2; Treated group of Haeca fermented product: HaCaT cells treated with the killed fermented product of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  1. Preparation of raw materials for fermentation and fermentation

The jujubea was prepared by crushing and harvesting from HyoSeongSeokjiro farm in Hadong area. The pulverized dead leaves were sun-dried and the completely dried tea was exposed to the outside at night when the dew was formed so that the natural fermentation proceeded for about one week. The naturally fermented tea was recovered and 5 g of killed carrot recovered in 1000 g of birch sap (Gangwon Province) was added and mixed. The mixture was placed in a fermenter, sterilized at about 100 ° C for about 5 minutes, and cooled to about 37 ° C to prepare a fermentation raw material.

Example  2. Primary fermentation of fermented fermented raw materials using lactic acid bacteria

The fermented fermented raw material prepared in Example 1 was fermented with lactic acid bacteria.

Specifically, Lactobacillus rhamnosus (KFCC11028) was directly isolated as a lactic acid bacterium. The above-mentioned lactic acid bacteria were cultured in a MRS (Man Rogosa Sharpe: BD) culture medium to obtain a culture solution. 200 ml of the culture solution of the lactic acid bacteria was inoculated such that the lactic acid bacteria were contained at about 1 × 10 ⁸ to about 5 × 10 ⁸ cells per about 1 kg of the fermentation raw material prepared in Example 1 above. After inoculation, fermentation was carried out for about 30 days under fermentation conditions of about 35 to about 37 DEG C, pH 7, stirring speed of about 100-120 rpm.

The fermented product thus obtained was centrifuged, and the precipitate and cells were removed to obtain a fermentation broth. 900 g of distilled water was added to the fermentation broth, and the mixture was heated at about 80 ° C. for about 6 hours in an extractor equipped with a cooling condenser, and 800 L of the distillate was recovered. The fermented product thus obtained was firstly filtered through a 300-mesh filter paper, allowed to stand at room temperature for 1 week, and then subjected to secondary filtration through a 150 mm filter paper to prepare a primary fermented product.

Example  3. Dead Fermented  Secondary fermentation

The primary fermentation product prepared in Example 2 was aged at low temperature for the second fermentation.

Specifically, after the first fermentation was stopped, the fermented product was recovered and the high pressure sterilized jade was immersed at about 100 DEG C for about 5 minutes. At this time, the fermented product and jade were moved to Onggi and the Onggi was kept in a thermostatic refrigerator maintained at a low temperature of about 4 ° C for about 70 days to perform a low-temperature aging to prepare a secondary fermented product.

Example  4. Dead Fermented  Produce

The secondary fermentation product prepared in Example 3 was centrifuged at about 14000 rpm for about 1 hour to remove precipitates and fungi as fermentation by-products, and only the fermentation broth was recovered. The supernatant was collected from the fermentation product obtained by centrifugation, and then subjected to a primary filtration with a 0.2 μm filter, followed by leaving at room temperature for about one week, and then subjected to a secondary filtration with a 0.2 μm filter to prepare a fermented product.

Experimental Example  1. Dead Fermented  Skin wound healing efficacy, skin barrier enhancement efficacy, moisturizing efficacy evaluation

Experiments were conducted to evaluate the skin wound healing efficacy, skin barrier enhancing efficacy, and moisturizing effect of the fermented fermented product prepared in Example 4 above.

Specifically, human keratinocyte HaCaT cells were cultured in DMEM medium containing 10% fetal bovine serum (Dulbecco's modified Eagle's Medium, Gibco 1210-0038), and cultured at 37 ° C, 5% CO ₂ incubator. The cultured cell line was treated with 1 mM of retinol as a positive control. The fermented broth was treated at a concentration of 1.0%.

Thereafter, HaCaT cells were further cultured for 24 hours, recovered, and RNA was isolated by adding 1 ml of trizol (RNA iso, DAKARA, Japan). RNA was quantified using Nanodrop 2000 (Thermo, USA) for RT-PCR, and cDNA was synthesized by reacting at 42 ° C for 55 minutes and 70 ° C for 15 minutes (Reverse Transcriptase Mix, ELPIS biotech, ).

RT-PCR confirmed the expression levels of filaggrin, HAS3 (hyaluronic acid synthase 3), and claudin 1 gene (genes related to skin wound healing, skin barrier enhancement and moisturization) by RT-PCR. PCR was carried out at 94 ° C for 5 min with primers and cDNAs, and PCR was performed at 95 ° C for 30 sec. , 54 ° C for 1 minute, and 72 ° C for 1 minute. The primers used are shown in Table 1 below. After completion of the polymerization, mRNA expression levels of clad 1, pilar green, and HAS3 genes were normalized to mRNA expression levels of β-actin, and relative expression levels were calculated.

gene SEQ ID NO: primer  designation primer  direction primer  order Claudin 1 One Claudin 1-F Forward 5'-GCTCTAGAATTCTCACACGTAGTCTTTCCCGCT-3 ' 2 Claudin 1-R Reverse 5'-GCTCTAGAATTCTCACACGTAGTCTTTCCCGCT-3 ' filagrin 3 filagrin-F Forward 5'-AGTGCACTCAGGGGGCTCACA-3 ' 4 filagrin -R Reverse 5'-CCGGCTTGGCCGTAATGTGT-3 ' HAS3 5 HAS3-F Forward 5'-CTTAAGGGTTGCTTGCTTGC-3 ' 6 HAS3-R Reverse 5'-GTTCGTGGGAGATGAAGGAA-3 ' β-Actin 7 Beta actin-F Forward 5'-AGAGCTACGAGCTGCCTGAC-3 ' 8 Beta actin-R Reverse 5'-AGCACTGTGTTGGCGTACAG-3 '

Figure 1 shows the relative levels of claudin 1 mRNA. From the left, the first bar is the HaCaT cells that have not been treated (control), the second to the fourth bars are the HaCaT cells treated with birch sap (birch sap treatment group); HaCaT cells treated with dead cells before fermentation (raw material treatment of fermented raw materials for fermentation); HaCaT cells treated with the primary fermentation product of killed fermented body prepared in Example 2 (primary fermented body treated with fermented tea); And relative mRNA levels of claudin 1 in HaCaT cells treated with the killed fermented product of the present invention (treated with fermented fermented products).

Figure 2 shows the relative levels of filagreen mRNA. From the left, the first bar is the HaCaT cells that have not been treated (control), the second to the fourth bars are the HaCaT cells treated with birch sap (birch sap treatment group); HaCaT cells treated with dead cells before fermentation (raw material treatment of fermented raw materials for fermentation); HaCaT cells treated with the primary fermentation product of killed fermented body prepared in Example 2 (primary fermented body treated with fermented tea); And the relative mRNA levels of pilar green in HaCaT cells treated with the killed fermented product of the present invention (treated with fermented fermented products).

Figure 3 shows the relative levels of HAS3 mRNA. From the left, the first bar is the HaCaT cells that have not been treated (control), the second to the fourth bars are the HaCaT cells treated with birch sap (birch sap treatment group); HaCaT cells treated with dead cells before fermentation (raw material treatment of fermented raw materials for fermentation); HaCaT cells treated with the primary fermentation product of killed fermented body prepared in Example 2 (primary fermented body treated with fermented tea); And the relative mRNA levels of HAS3 in HaCaT cells treated with the killed fermented product of the present invention (group treated with fermented fermented products).

As shown in FIG. 1 to FIG. 3, when human keratinocyte cell line was treated with a fermented fermentation raw material or a primary fermented product to die, the factors related to skin wound healing, skin barrier strengthening and moisturizing were clathrin 1, pilagreen, and HAS3 gene It was confirmed that the level of relative mRNA expression was similar or lower than that of control birch sap. On the other hand, the fermented fermented product according to the present invention exhibits a significantly higher mRNA expression level than the other substances, and shows excellent skin wound healing effect, skin barrier strengthening effect and skin moisturizing effect.

<110> Cosmax Co., Ltd. <120> Method for preparing tea fermented materials and cosmetic          composition comprising the same <130> PN120339 <160> 8 <170> KoPatentin 3.0 <210> 1 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Claudin 1-F primer <400> 1 gctctagaat tctcacacgt agtctttccc gct 33 <210> 2 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> Claudin 1-R primer <400> 2 gctctagaat tctcacacgt agtctttccc gct 33 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> filagrin-F primer <400> 3 agtgcactca gggggctcac a 21 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> filagrin-R primer <400> 4 ccggcttggc cgtaatgtgt 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS3-F primer <400> 5 cttaagggtt gcttgcttgc 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> HAS3-R primer <400> 6 gttcgtggga gatgaaggaa 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Beta actin-F primer <400> 7 agagctacga gctgcctgac 20 <210> 8 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Beta actin-R primer <400> 8 agcactgtgt tggcgtacag 20

Claims (11)

A cosmetic composition for improving skin comprising an active ingredient of a fermented killed fermented soybean fermented with lactic acid bacteria. The method of claim 1, wherein the lactic acid bacterium is selected from the group consisting of Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus acidophilus, Wherein the composition is selected from the group consisting of Lactobacillus acidophilus , Lactobacillus helveticus , and Lactobacillus delbrueckii subspecies bulgaricus . [Claim 3] The cosmetic composition according to claim 1, wherein the killed fermented product is fermented with lactic acid bacteria at a temperature ranging from 100 rpm to 150 rpm for 35 to 35 days at 35 [deg.] C to 37 [deg.] C. [Claim 6] The cosmetic composition according to claim 1, wherein the killed fermented product is fermented with lactic acid bacteria, followed by dipping the jute and further fermenting at 2 to 7 [deg.] C for 65 to 75 days. The cosmetic composition according to claim 1, wherein the killed fermented product is contained in an amount of 0.001 wt% to 10 wt% based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, which has a wound healing effect, a skin barrier strengthening effect, or a moisturizing effect. The cosmetic composition according to claim 1, wherein expression of claudin 1, filagrin, or HAS3 (hyaluronic acid synthase 3) is increased. Preparing a first fermented product by fermenting the fermented milk with lactic acid bacteria; And
A step of immersing the primary fermentation product in an oven and aging at a low temperature to prepare a secondary fermentation product. The method of claim 8, wherein the lactic acid bacterium is selected from the group consisting of Lactobacillus rhamnosus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus plantarum , Lactobacillus acidophilus, Lactobacillus acidophilus , Lactobacillus helveticus , and Lactobacillus delbrueckii subspecies bulgaricus . [Claim 9] The method according to claim 8, wherein the fermentation in the step of preparing the primary fermentation product is performed at a temperature of 35 [deg.] C to 37 [deg.] C for 25 to 35 days at 100 rpm to 150 rpm. The method according to claim 8, wherein aging in the step of producing the secondary fermentation product is performed at 2 ° C to 7 ° C for 65 days to 75 days.

Images