CN212589313U - Inoculation device of liquefied strain - Google Patents
Inoculation device of liquefied strain Download PDFInfo
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- CN212589313U CN212589313U CN201922471757.XU CN201922471757U CN212589313U CN 212589313 U CN212589313 U CN 212589313U CN 201922471757 U CN201922471757 U CN 201922471757U CN 212589313 U CN212589313 U CN 212589313U
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Abstract
The utility model discloses an inoculation device for liquefied strains; the inoculation device is structurally characterized in that a container is placed on a magnetic stirrer, the magnetic stirrer is arranged at the bottom of the container, a liquid suction pipe and a liquid conveying pipe are connected together through a connecting pipe, the connecting pipe penetrates through a bottle stopper, the bottle stopper is fixed at the bottle opening of the container, the liquid suction pipe extends into the position close to the bottom of the container, the other end of the liquid conveying pipe is connected with a seed receiving pipe, and the middle part of the liquid conveying pipe is fixed on a roller of a distribution type peristaltic pump. The utility model discloses inoculation device simple structure, simple manufacture, with low costs, easy operation are suitable for the agricultural formula cultivation of solid liquefaction bacterial.
Description
Technical Field
The utility model belongs to an edible mushroom inoculation device, in particular to an inoculation device for solid liquefied strains.
Background
A solid liquefied strain (also called as reduced liquid strain) is a new method for preparing edible fungus strain invented by Japan original biotechnology, and is characterized by that firstly, the strain is made into solid block-shaped hypha, before inoculation the block-shaped hypha is broken, and then the solid block-shaped hypha is diluted with sterile water to obtain the liquid strain. The solid liquefied strain has the advantages of short production period, fast hypha growth, consistent fungus age, regular fruiting, suitability for large-scale industrial production and the like of the liquid strain, and overcomes the defects of high equipment requirement, high pollution risk, intolerance of storage, inconvenient transportation and the like of the liquid strain, so the solid liquefied strain is more and more widely applied. However, the inoculation device for the liquid spawn currently used has the defects of complex structure, high price, inconvenient operation and the like, and limits the application of the liquid spawn or the solid liquefied spawn to the agricultural cultivation of the edible fungi.
The patent "a simple liquid seed culture inoculating device" (CN203057903U) discloses a simple liquid seed culture inoculating device, but it has the following disadvantages: (1) an air pump is needed, if the air pump is not used properly, the cylinder, the air storage cylinder, the exhaust pipe and the like of the inflation pump can burst, and injury accidents are easy to happen; (2) the effect of filtering air by cotton is not good, the filtered air can be sterile only when the aperture of the microwave filter element of the air filter is less than 0.22 μm, and the air entering the glass bottle after being filtered cannot be sterile by the filter made of cotton.
SUMMERY OF THE UTILITY MODEL
To the problem that present solid liquefaction bacterial inoculation device exists, the utility model aims to provide an inoculation device of solid liquefaction bacterial.
In order to achieve the above purpose, the technical scheme of the utility model is as follows:
the inoculation device for the solid liquefied strains is structurally composed of a magnetic stirrer 1, a magnetic stirrer 2, a container 3, a liquid suction pipe 4, a bottle stopper 5, a connecting pipe 6, a liquid conveying pipe 7, a peristaltic pump 8 and a seed receiving pipe 9; wherein, place container 3 on magnetic stirrers 1, magnetic stirrers 2 is in the bottom of container 3, and pipette 4 and transfer line 7 link together through connecting pipe 6, and connecting pipe 6 passes bottle plug 5, and bottle plug 5 is fixed in the bottleneck department of container 3, and pipette 4 stretches into the bottom position that is close to container 3, and the other end and the inoculation pipe 9 of transfer line 7 are connected, and the mid portion of transfer line 7 is fixed in on the gyro wheel of distribution type peristaltic pump 8.
The container 3 in the inoculation device is a glass bottle or a stainless steel bottle.
The connecting pipe 6 or the inoculation pipe 9 in the inoculation device is made of stainless steel.
The pipette 4 or the infusion tube 7 in the inoculation device is made of silica gel.
In the inoculation device, the bottle stopper 5 is made of silica gel.
The length of the infusion tube 7 in the inoculation device is 2.5-3 m.
The length of the inoculation tube 9 in the inoculation device is 10-15 cm.
The dispensing peristaltic pump of the inoculating device can be purchased from the market, such as Lange BT300-1F dispensing peristaltic pump manufactured by Baoding Lange constant flow pump company Limited.
The inoculation device described above requires sterilization of all the parts, except the magnetic stirrer and the dispensing peristaltic pump, and operates under aseptic conditions.
Compared with the prior art, the utility model has the advantages and beneficial effect: (1) the utility model provides a simple inoculating device for agricultural cultivation of solid liquefied strains, the utility model has simple manufacture and low cost, and when only inoculating 1 variety, the cost is 1/3 when using the fermentation tank; when a plurality of varieties are inoculated, the cost of using the utility model is only about 1/10 of the cost of using the fermentation tank; (2) the utility model is suitable for simultaneously cultivating a plurality of varieties under the condition of low investment; (3) the utility model discloses inoculation device easy operation. The operator only needs to master basic microorganism sterile operation knowledge, and can master the operation process by actually watching the operation knowledge once without special technical training.
Description of the drawings:
FIG. 1 is a schematic view of an inoculating device of the present invention; wherein 1 is a magnetic stirrer, 2 is a magnetic stirrer, 3 is a container, 4 is a pipette, 5 is a bottle stopper, 6 is a connecting pipe, 7 is an infusion pipe, 8 is a peristaltic pump, and 9 is a inoculation pipe.
Detailed Description
EXAMPLE 1 manufacture of the inoculating device of the present invention
The preparation method comprises the following steps: according to the figure 1, a container 3 is placed on a magnetic stirrer 1, a magnetic stirrer 2 is arranged at the bottom of the container 3, a liquid suction pipe 4 and a liquid conveying pipe 7 are connected together through a connecting pipe 6, the connecting pipe 6 penetrates through a bottle stopper 5, the bottle stopper 5 is fixed at the bottle mouth of the container 3, the liquid suction pipe 4 extends to a position close to the bottom of the container 3, the other end of the liquid conveying pipe 7 is connected with a liquid receiving pipe 9, and the middle part of the liquid conveying pipe 7 is fixed on a roller of a distribution type peristaltic pump 8; wherein the pipette 4 and the infusion tube 7 are silicone tubes, the connecting tube 6 and the inoculation tube 9 are stainless steel tubes, and the bottle stopper 5 is made of silicone plugs. Before manufacturing, all the parts except the magnetic stirrer 1 and the distribution type peristaltic pump 8 need to be sterilized at 121 ℃ for 30 min.
EXAMPLE 2 method of using the inoculating device of the present invention
The method comprises the following steps:
(1) an inoculation device was made as described in example 1;
(2) preparing a seed slurry according to the method disclosed in patent application CN 110036829A; under the aseptic condition, adding aseptic water into a container 3, then pouring the strain slurry into the aseptic water according to the dilution ratio, covering a bottle stopper 5, stirring the strain solution by using a magnetic stirrer 1 and a magnetic stirrer 2 at the rotating speed of 60rpm, and controlling the bottom to have no precipitate so as to ensure that the strain solution is uniform;
(3) starting the peristaltic pump 8, conveying the bacterial liquid sucked from the container 3 by the liquid suction pipe 4 to the liquid conveying pipe 7 through the connecting pipe 6, and conveying the bacterial liquid to the inoculation pipe 9 through the peristaltic pump 8; inoculating the bacterial liquid to the center of a sterilized bacterial bag under aseptic conditions, wherein the inoculation amount is 25 ml/bag, and sealing the bag by using a non-cotton cover body after inoculation;
(4) culturing the inoculated fungus bags in a clean environment at 23 ℃ in the dark for 12 days until the fungus bags grow full, and entering a fruiting management period if no pollution exists.
Claims (7)
1. The inoculation device for the liquefied strains is characterized by comprising a magnetic stirrer (1), a magnetic stirrer (2), a container (3), a pipette (4), a bottle stopper (5), a connecting pipe (6), a transfusion pipe (7), a peristaltic pump (8) and a inoculation pipe (9); wherein, place container (3) on magnetic stirrers (1), magnetic stirrers (2) are in the bottom of container (3), pipette (4) and transfer line (7) link together through connecting pipe (6), connecting pipe (6) pass bottle plug (5), bottle plug (5) are fixed in the bottleneck department of container (3), pipette (4) stretch into the bottom position that is close to container (3), the other end and the inoculation pipe (9) of transfer line (7) are connected, the mid portion of transfer line (7) is fixed in on the gyro wheel of distribution type peristaltic pump (8).
2. The inoculating device according to claim 1, wherein the container (3) is a glass bottle or a stainless steel bottle.
3. An inoculating device according to claim 1, characterised in that the connecting tube (6) or the connecting tube (9) is of stainless steel.
4. The inoculating device according to claim 1, wherein the pipette (4) or the infusion tube (7) is made of silica gel.
5. The inoculating device according to claim 1, wherein the plug (5) is made of silica gel.
6. The inoculating device according to claim 1, wherein the length of the infusion tube (7) is 2.5-3 m.
7. An inoculating device according to claim 1, characterised in that the length of the inoculation tube (9) is 10-15 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922471757.XU CN212589313U (en) | 2019-12-31 | 2019-12-31 | Inoculation device of liquefied strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922471757.XU CN212589313U (en) | 2019-12-31 | 2019-12-31 | Inoculation device of liquefied strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212589313U true CN212589313U (en) | 2021-02-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922471757.XU Expired - Fee Related CN212589313U (en) | 2019-12-31 | 2019-12-31 | Inoculation device of liquefied strain |
Country Status (1)
| Country | Link |
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| CN (1) | CN212589313U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114246106A (en) * | 2021-11-15 | 2022-03-29 | 江苏品品鲜生物科技有限公司 | Method for crushing velvet antler mushroom strains |
-
2019
- 2019-12-31 CN CN201922471757.XU patent/CN212589313U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114246106A (en) * | 2021-11-15 | 2022-03-29 | 江苏品品鲜生物科技有限公司 | Method for crushing velvet antler mushroom strains |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210226 Termination date: 20211231 |
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| CF01 | Termination of patent right due to non-payment of annual fee |