CN103518534B - Edible fungus liquid strain storing and inoculating integrated machine - Google Patents
Edible fungus liquid strain storing and inoculating integrated machine Download PDFInfo
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- CN103518534B CN103518534B CN201310481009.4A CN201310481009A CN103518534B CN 103518534 B CN103518534 B CN 103518534B CN 201310481009 A CN201310481009 A CN 201310481009A CN 103518534 B CN103518534 B CN 103518534B
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Abstract
The invention discloses an edible fungus liquid strain storing and inoculating integrated machine. The edible fungus liquid strain storing and inoculating integrated machine is formed by connection of a storing device and an inoculating device. The storing device comprises a liquid strain storage bottle, a bottle cap and an O-shaped seal ring, wherein the O-shaped seal ring is arranged between the bottle neck and the bottle cap of the liquid strain storage bottle. The inoculating device comprises an inoculating syringe needle, a bend and a silicon rubber hose, wherein the inoculating syringe needle is connected with the silicon rubber hose through the bend, a hole is formed in the bottle cap, the bend and the silicon rubber hose penetrate through the hole to be inserted into the liquid strain storage bottle, and the external diameter of the bend is matched with the internal diameter of the hole. The edible fungus liquid strain storing and inoculating integrated machine has the advantages that the structure is simple, storing and inoculating of liquid strains are integrated, operation is simple and convenient, the inoculating speed is high, labor intensity of workers can be relieved obviously, and production efficiency is improved.
Description
Technical field
The present invention relates to a kind of edible fungi liquid strain storage inoculating tool, specifically a kind of edible fungi liquid strain stores inoculation all-in-one.
Background technology
" edible fungi liquid strain " refer to produced by deep drainpipe technology with liquid nutrient medium in biological fermentation tank there is fertility, the propagating materials of the mycelium of the metastable edible mushroom of hereditary capacity and trophism and non-nutritive fluid carrier medium composition.Liquid spawn is applied to the production of edible mushroom, for mushroom industry from the very complicated traditional mode of production, the cycle is long, cost is high, by rule of thumb, spell labour, handicraft workshop formula to automation, standardization, scale upgrading be significant.
Mid-term in 20th century, the fast development of microbial liquid deep drainpipe technology, thus define modern medicine, food fermentation industry.American scientist Humfeld in 1948 etc. have successfully carried out Submerged liquid culturation to mushroom, opened the mankind to carry out edible fungus liquid and cultivate and the prelude of liquid spawn research, Hen Kuaishi American-European countries Mushroom production five, sixties quick step enters liquid spawn and industrial cultivation epoch.
At present, world's biofermentation rate of industrial development is surprising, and the edible fungi liquid strain of Britain is produced and represented West Europe and North America developed country bacterial classification production model.The mushroom of Britain adopts full-automatic bulk fermentation to prepare liquid spawn, and national bacterial classification is only completed by the international bacterial classification company of Sylvon and Amycel Liang great.
In recent years, the research application of the Asian countries such as Japan, Korea S to mushroom, Asparagus, flat mushroom liquid spawn is very deep, and existing many mushroom factories start to produce liquid spawn with middle-size and small-size fermentation tank and cultivate.
What China was comparatively early engaged in edible fungus liquid submerged fermentation research is that Shanghai plant physiology research institute Sun Mei then waits people, has carried out mushroom liquid culture studies in nineteen sixty.The Yang Qingyao professor of Shanghai in 1979 teachers college starts to cultivate liquid spawn to mushroom, Asparagus etc., and carries out experiment in cultivation.From the century eighties to 20, the technical staff of many scientific research institutions of China starts to carry out repeatedly edible fungi submerged fermentation, liquid spawn and middle-size and small-size fermentation equipment, deep research, for China liquid spawn provides valuable experience at fermentation equipment, technology and growth condition, establish solid foundation.At present, the liquid spawn seen produces has about more than 50 the rare kind such as the conventional varieties such as flat mushroom, mushroom, woodear, White mushroom and Pleuotus nebrodensis Quel, Xingbao mushroom, true Ji mushroom with the edible fungus variety of application.Calendar year 2001, Nanchen Tianhong Agricultural Art Co., Ltd., Bejing takes the lead at home developing and uses the liquid mode of continuously fermenting to produce edible bacterium and succeeds and obtain obvious benefit, nine, Shandong edible mushroom Co., Ltd had reached the production scale of annual output 1000 ten thousand liters of liquid spawns already, indicated that liquid spawn has been applied in fairly large production.
The application of liquid spawn in edible fungus culturing can be rated as the revolution of Edible Fungi, and it represents the developing direction of Edible Fungi technology, has fully demonstrated the feature that edible fungi liquid strain is with short production cycle, biological efficiency is high.Present stage edible fungi liquid strain production and application generally there being batch production, automated production equipment large enterprise carries out.But liquid culture production equipment and cultivation production line are invested huge, and technical requirement is high.
At present, the most of mushroom agriculture working condition of China does not possess the ability of investment industrial production line, local economy and ecotope can only be relied on to carry out traditional agricultural and the simple and mechanical mode combined is produced, in the use of liquid spawn, often run into and store the practical problems such as traffic condition is high, inoculation difficulty is large, and existing inoculating tool manufacturing cost is high, complicated operation, the demand of common mushroom agriculture can not be met, hinder the popularization of liquid spawn.
Summary of the invention
The object of this invention is to provide a kind of edible fungi liquid strain and store inoculation all-in-one, to solve, existing inoculating tool manufacturing cost is high, complicated operation, can not the problem of Simultaneous vaccination and storage.
Technical solution of the present invention is as follows: a kind of edible fungi liquid strain stores inoculation all-in-one, it is formed by connecting by storage device and classification inoculation apparatus, described storage device comprises liquid spawn storage bottle, bottle cap and O RunddichtringO, is provided with O RunddichtringO between the bottleneck of described liquid spawn storage bottle and bottle cap; Described classification inoculation apparatus comprises inoculation syringe needle, elbow and silicon rubber hose, described inoculation syringe needle is connected with silicon rubber hose by elbow, described bottle cap is provided with hole, described elbow and silicon rubber hose insert in described liquid spawn storage bottle through described hole, the external diameter of described elbow and the internal diameter in described hole suitable.
As a further improvement on the present invention, the bottle inner bottom part of described liquid spawn storage bottle is provided with location Liquid absorption ball, the center of described location Liquid absorption ball is provided with hollow pipe, and the bottom of described silicon rubber hose is inserted at the top of described hollow pipe, and the bottom of described location Liquid absorption ball is provided with at least one liquid sucting port.
As a further improvement on the present invention, the port of described inoculation syringe needle is provided with sealing cap.
The present invention in order to the equipment investment solved in edible fungi liquid strain use procedure large, store and inoculation difficulty, the bottleneck problem of existing equipment operating complexity, the edible mushroom bag that the actual and China of production for the vast mushroom agriculture in remote rural area and small-sized Edible Fungi enterprise generally adopts at present plants mode, on traditional liquid-spawn inoculation method basis, a kind of collection is provided to store, transport and inoculation are integrated, easy and simple to handle, can continuous inoculation, transfer needle is replaceable, inoculation speed is fast, connect bacterium success rate high, be conducive to reducing labour intensity, the edible fungi liquid strain realizing high-efficiency reform stores inoculation all-in-one, its advantage is: 1. its inoculation speed is fast, labour intensity is little, production efficiency can be significantly improved, 2. entirety can carry out high pressure steam sterilization, meet sterile working needs completely, effect of inoculation is reliable.3. can be finished liquid spawn in bottle completely, not remain, do not waste.4. cheap for manufacturing cost, reusable, significantly can reduce producing cost.
Accompanying drawing explanation
Fig. 1 is the structural representation that a kind of edible fungi liquid strain stores inoculation all-in-one;
Fig. 2 is that a kind of edible fungi liquid strain stores the structural representation of locating Liquid absorption ball in inoculation all-in-one.
Reference numeral implication is as follows: liquid spawn storage bottle (1), location Liquid absorption ball (2), silicon rubber hose (3), bottle cap (4), O RunddichtringO (5), elbow (6), inoculation syringe needle (7), sealing cap (8), hollow pipe (10), liquid sucting port (11).
Embodiment
The present invention may be better understood for embodiment below, but do not limit the present invention in any way.
A kind of edible fungi liquid strain stores inoculation all-in-one, and it is formed by connecting by storage device and classification inoculation apparatus, and storage device comprises liquid spawn storage bottle 1, bottle cap 4 and O RunddichtringO 5, is provided with O RunddichtringO 5 between the bottleneck of liquid spawn storage bottle 1 and bottle cap 4; Classification inoculation apparatus comprises inoculation syringe needle 7, elbow 6 and silicon rubber hose 3, inoculation syringe needle 7 is connected with silicon rubber hose 3 by elbow 6, bottle cap 4 is provided with hole 9, and elbow 6 inserts in liquid spawn storage bottle 1 with silicon rubber hose 3 through hole 9, the external diameter of elbow 6 and the internal diameter in hole 9 suitable.The bottle inner bottom part of liquid spawn storage bottle 1 is provided with location Liquid absorption ball 2, and the center of location Liquid absorption ball 2 is provided with hollow pipe 10, and the bottom of silicon rubber hose 3 is inserted at the top of hollow pipe 10, and the bottom of location Liquid absorption ball 2 is provided with at least one liquid sucting port 11.The port of inoculation syringe needle 7 is provided with sealing cap 8.The bottle lateral wall of liquid spawn storage bottle 1 indicates volume scale.
Liquid sucting port 11 is 4 in the present embodiment, and an angle of 90 degrees of being separated by each other is distributed in the bottom surrounding of location Liquid absorption ball 2.Be threaded between elbow 6 with inoculation syringe needle 7.Also for being threaded between elbow 6 with bottle cap 4.Liquid spawn stored vial 1 is made up of polypropylene copolymer or fluorinated ethylene propylene copolymer material.The material of location Liquid absorption ball 2 is 304 stainless steels.
It is a kind of inoculating tool for shifting edible fungi liquid strain that edible fungi liquid strain stores inoculation all-in-one, during use, first edible fungi liquid strain storage inoculation all-in-one is placed in pressure steam sterilizer and carries out sterilizing, then the mode of liquid spawn sterile working is loaded in liquid spawn storage bottle 1, load onto the classification inoculation apparatus be connected with bottle cap 4, after inoculation opening part puts on sealing cap 8, transport to planting edible mushroom inoculation place, open sealing cap 8, inoculation syringe needle 7 inserted in composts or fertilisers of cultivating mushroom or aim at composts or fertilisers of cultivating surface, the liquid spawn liquid storage bottle 1 of pressing to be become by PPCO (polypropylene copolymer) or the materials processing of FEP fluorinated ethylene propylene copolymer is pinched with hand, a bottle internal volume is reduced, pressure strengthens, gravitate edible fungi liquid strain stores inoculation all-in-one and is in any position pleasant relative to operator, in bottle, liquid spawn is all in relative low level, location Liquid absorption ball 2 is made up of stainless steel, it is than great, gravitate is in the relative low level of bottle 1 inside all the time, 4 entrances are arranged at bottom, be distributed in bottom surrounding, be separated by between entrance 90 ° and arrange, no matter which kind of position bottle is in, 1 entrance is always had to soak in liquid spawn again, in bottle, the liquid spawn effect of being stressed can only be pressed into from 4 or any 1 porch of location Liquid absorption ball 2, inoculation syringe needle 7 is entered by silicone rubber tube 3 and elbow 6, not residual or seldom residual, do not waste, finally make liquid spawn flow into composts or fertilisers of cultivating from inoculation syringe needle 7, complete the transfer of liquid spawn from storage bottle to composts or fertilisers of cultivating mushroom, namely complete the storage of edible fungi liquid strain, transport and seeded process, such continued operation, until liquid spawn uses until exhausted in bottle.
FEP(fluorinated ethylene propylene copolymer) or PPCO (polypropylene copolymer) materials processing become liquid spawn liquid storage bottle, tool still can keep elasticity and pliability after high temperature and high pressure steam sterilizing, after ambient pressure effect distortion, rapid reinstatement, the size of power determine inoculum concentration number, can reach fast and the object of relative quantification; Storing inoculation all-in-one material therefor can high temperature and high pressure steam sterilizing, and sterilization effect is reliable.
Claims (9)
1. an edible fungi liquid strain stores inoculation all-in-one, it is characterized in that: it is formed by connecting by storage device and classification inoculation apparatus, described storage device comprises liquid spawn storage bottle (1), bottle cap (4) and O RunddichtringO (5), is provided with O RunddichtringO (5) between the bottleneck of described liquid spawn storage bottle (1) and bottle cap (4); Described classification inoculation apparatus comprises inoculation syringe needle (7), elbow (6) and silicon rubber hose (3), described inoculation syringe needle (7) is connected with silicon rubber hose (3) by elbow (6), described bottle cap (4) is provided with hole (9), described elbow (6) and silicon rubber hose (3) insert in described liquid spawn storage bottle (1) through described hole (9), the external diameter of described elbow (6) and the internal diameter of described hole (9) suitable.
2. edible fungi liquid strain according to claim 1 stores inoculation all-in-one, it is characterized in that: the bottle inner bottom part of described liquid spawn storage bottle (1) is provided with location Liquid absorption ball (2), the center of described location Liquid absorption ball (2) is provided with hollow pipe (10), the bottom of described silicon rubber hose (3) is inserted at the top of described hollow pipe (10), and the bottom of described location Liquid absorption ball (2) is provided with at least one liquid sucting port (11).
3. edible fungi liquid strain according to claim 2 stores inoculation all-in-one, it is characterized in that: described liquid sucting port (11) is 4, and an angle of 90 degrees of being separated by each other is distributed in the bottom surrounding of location Liquid absorption ball (2).
4. edible fungi liquid strain according to claim 3 stores inoculation all-in-one, it is characterized in that: the port of described inoculation syringe needle (7) is provided with sealing cap (8).
5. edible fungi liquid strain according to claim 4 stores inoculation all-in-one, it is characterized in that: be threaded between described elbow (6) with described inoculation syringe needle (7).
6. edible fungi liquid strain according to claim 5 stores inoculation all-in-one, it is characterized in that: the bottle lateral wall of described liquid spawn storage bottle (1) indicates volume scale.
7. edible fungi liquid strain according to claim 6 stores inoculation all-in-one, it is characterized in that: be threaded between described elbow (6) with bottle cap (4).
8. edible fungi liquid strain according to claim 7 stores inoculation all-in-one, it is characterized in that: described liquid spawn stored vial (1) is made up of polypropylene copolymer or fluorinated ethylene propylene copolymer material.
9. edible fungi liquid strain according to claim 8 stores inoculation all-in-one, it is characterized in that: the material of described location Liquid absorption ball (2) is 304 stainless steels.
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| CN201310481009.4A CN103518534B (en) | 2013-10-15 | 2013-10-15 | Edible fungus liquid strain storing and inoculating integrated machine |
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| CN201310481009.4A CN103518534B (en) | 2013-10-15 | 2013-10-15 | Edible fungus liquid strain storing and inoculating integrated machine |
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| CN103518534B true CN103518534B (en) | 2015-03-18 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105087750A (en) * | 2014-05-23 | 2015-11-25 | 大连富智森生物科技有限公司 | Edible fungus liquid strain detector |
| CN104054510B (en) * | 2014-06-23 | 2016-04-13 | 中华全国供销合作总社昆明食用菌研究所 | A kind of edible fungi liquid strain seeding apparatus |
| CN108433849A (en) * | 2018-04-18 | 2018-08-24 | 浙江康瑞器械科技股份有限公司 | A kind of continuous medicine-filling injector |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209482U (en) * | 1987-06-23 | 1988-07-13 | 余兆丰 | Liquid spawn mulfitube-inoculator for bag-carried edible fungus |
| CN2770316Y (en) * | 2005-03-15 | 2006-04-12 | 罗孝名 | Efficient liquid bacterial bottle device |
| CN101153265B (en) * | 2007-08-29 | 2010-11-17 | 浙江大学 | Safe and portable liquid bacterial vaccinating device |
| CN203537923U (en) * | 2013-10-15 | 2014-04-16 | 甘肃省科学院生物研究所 | Edible mushroom liquid strain storing and inoculating all-in-one machine |
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2013
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209482U (en) * | 1987-06-23 | 1988-07-13 | 余兆丰 | Liquid spawn mulfitube-inoculator for bag-carried edible fungus |
| CN2770316Y (en) * | 2005-03-15 | 2006-04-12 | 罗孝名 | Efficient liquid bacterial bottle device |
| CN101153265B (en) * | 2007-08-29 | 2010-11-17 | 浙江大学 | Safe and portable liquid bacterial vaccinating device |
| CN203537923U (en) * | 2013-10-15 | 2014-04-16 | 甘肃省科学院生物研究所 | Edible mushroom liquid strain storing and inoculating all-in-one machine |
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