CN202576404U - Experiment device for anaerobic microorganism culture - Google Patents
Experiment device for anaerobic microorganism culture Download PDFInfo
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- CN202576404U CN202576404U CN 201220193690 CN201220193690U CN202576404U CN 202576404 U CN202576404 U CN 202576404U CN 201220193690 CN201220193690 CN 201220193690 CN 201220193690 U CN201220193690 U CN 201220193690U CN 202576404 U CN202576404 U CN 202576404U
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- pipe
- pot body
- anaerobic
- rubber pipe
- air
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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Abstract
The utility model discloses an experiment device for anaerobic microorganism culture. The experiment device comprises a pot body, a vacuum meter, an air-guiding pipe and a rubber pipe, wherein a screwed cover is arranged on the upper portion of the pot body, the vacuum meter and the air-guiding pipe are arranged on the upper portion of the screwed cover, a sealing ring is disposed on a connecting portion of the screwed cover and the pot body, a sleeve pipe is arranged in the pot body, culture dishes and the rubber pipe are arranged on the sleeve pipe, the rubber pipe is connected with the air-guiding pipe, a water-stopping clamp is arranged on a loop of the rubber pipe, and the pot body is plastic and transparent. When the experiment device is used, the screwed cover of an anaerobic pot is opened, culture dishes containing culturing medium are placed in a sleeve barrel in sequence, then the sleeve barrel is placed in the anaerobic pot body, and the rubber sealing ring and the screwed cover are covered. An air-exhausting opening of a vacuum pump is connected with the rubber pipe, and the water-stopping clamp is opened. When the vacuum pump displays certain vacuum, the water-stopping clamp is closed, the vacuum pump and the rubber pipe are disconnected, the whole anaerobic pot is placed in a culturing box, culturing conditions are set, and therefore the anaerobic culture is carried out. The experiment has the advantages of simple operation, convenient use and low cost.
Description
Technical field
The utility model relates to a kind of microbial cultivation device, has to relate to a kind of experimental installation that anaerobion is cultivated that is used for.
Background technology
Anaerobion is extensive in distributed in nature, and is of a great variety, and effect also draws attention day by day.The key problem in technology of cultivating anaerobion is how to make this quasi-microorganism in process of growth, be in the environment of anaerobic or hypoxemia.At present; Popular anaerobion cultural method is through particular chemical reagent the oxygen in a certain enclosed space completely or partially to be sponged; Produce carbonic acid gas then; According to the different needs of laboratory culture anaerobion, develop that complete anaerobism is cultivated, little aerobic cultivation and have a liking for different type such as carbonic acid gas cultivation.After adopting pyrogallol and basic soln effect, form alkaline gallate, in this reaction process, can absorb oxygen and cause anaerobic environment.
At present existing patent report adopts the anaerobism bag to remove the oxygen of container.The anaerobic gas generation bag is compared with other anaerobism cultural methods; Have easy and simple to handle, without catalyzer, advantage such as with strong points, but can not recycle after each anaerobism bag uses up, can only give up; And the cost of anaerobism bag is higher; If frequency of utilization is too high, will certainly cause bigger economical load, common laboratory is reluctant to accept.
Summary of the invention
The technical problem that the utility model will solve provides a kind of experimental installation that anaerobion is cultivated, characteristics simple to operate, easy to use, with low cost of being used for.
For achieving the above object, the technical scheme of the utility model is following:
A kind of experimental installation that is used for the anaerobion cultivation comprises tank body, and said tank body top is provided with spiral cover, is arranged at the vacuum meter and the gas tube on spiral cover top.
Preferably, the junction of said spiral cover and tank body also is provided with sealing-ring.
Preferably, said tank interior is provided with sleeve pipe, disposes petridish on the sleeve pipe.
Preferably, the rubber hose that links to each other with gas tube disposes tongs on the loop of said rubber hose.
Preferably, said tank body is the plastic, transparent shape.
Through technique scheme, the beneficial effect of the utility model is:
1, plastic seal tank body appearance transparent can be observed the microorganism growth situation at any time;
2, need not add water and place any reagent, vacuumize after the sealing and can form suitable anaerobism, little environment such as aerobic;
3, do not have offal treatment in the culturing process, do not pollute the environment;
4, economical and practical, as need not to use costliness and take up space main equipment.
Description of drawings
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art; To do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art below; Obviously, the accompanying drawing in describing below only is some embodiment of the utility model, for those of ordinary skills; Under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of the utility model.
Embodiment
For technique means, creation characteristic that the utility model is realized, reach purpose and be easy to understand understanding with effect, below in conjunction with concrete diagram, further set forth the utility model.
A kind of experimental installation that is used for the anaerobion cultivation comprises tank body 6, and said tank body 6 tops are provided with spiral cover 2, is arranged at the vacuum meter 1 and gas tube 7 on spiral cover 2 tops.
Preferably, said spiral cover 2 also is provided with sealing-ring 3 with the junction of tank body 6.
Preferably, said tank body 6 set inside have sleeve pipe 5, dispose petridish 4 on the sleeve pipe 5.
Preferably, the rubber hose 9 that links to each other with gas tube 7 disposes tongs 8 on the loop of said rubber hose 9.
Preferably, said tank body 6 is the plastic, transparent shape.
Said vacuum meter 1, gas tube 7 and spiral cover 2 are a welding integral, during use, open the anaerobic jar spiral cover, 2; The petridish that fills substratum 4 is put into sleeve 5 successively, then sleeve 5 is put into anaerobic jar tank body 6, cover rubber seal 3 and spiral cover 2, connect rubber hose 9 with the vacuum pump bleeding point; Open tongs 8,, close tongs 8, break off being connected of vacuum pump and rubber hose 9 when vacuum pump shows to certain vacuum; Use nitrogengas cylinder to be connected, open tongs 8, pour nitrogen with rubber hose 9; After the vacuum pump displayed value is zero, close tongs 8, break off being connected of nitrogengas cylinder and rubber hose 9; Whole anaerobic jar is put into incubator, set culture condition, can carry out anaerobism and cultivate.
More than show and described ultimate principle of the utility model and the advantage of principal character and the utility model.The technician of the industry should understand; The utility model is not restricted to the described embodiments; The principle of describing in the foregoing description and the specification sheets that the utility model just is described; Under the prerequisite that does not break away from the utility model spirit and scope, the utility model also has various changes and modifications, and these variations and improvement all fall in the utility model scope that requires protection.The utility model requires protection domain to be defined by appending claims and equivalent thereof.
Claims (5)
1. one kind is used for the experimental installation that anaerobion is cultivated, and comprises tank body, it is characterized in that said tank body top is provided with spiral cover, is arranged at the vacuum meter and the gas tube on spiral cover top.
2. a kind of experimental installation that anaerobion is cultivated that is used for according to claim 1, it is characterized in that: the junction of said spiral cover and tank body also is provided with sealing-ring.
3. a kind of experimental installation that anaerobion is cultivated that is used for according to claim 1 and 2, it is characterized in that: said tank interior is provided with sleeve pipe, disposes petridish on the sleeve pipe.
4. a kind of experimental installation that anaerobion is cultivated that is used for according to claim 1 is characterized in that: the rubber hose that links to each other with gas tube, dispose tongs on the loop of said rubber hose.
5. a kind of experimental installation that anaerobion is cultivated that is used for according to claim 1 and 2, it is characterized in that: said tank body is the plastic, transparent shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220193690 CN202576404U (en) | 2012-04-28 | 2012-04-28 | Experiment device for anaerobic microorganism culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220193690 CN202576404U (en) | 2012-04-28 | 2012-04-28 | Experiment device for anaerobic microorganism culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202576404U true CN202576404U (en) | 2012-12-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220193690 Expired - Fee Related CN202576404U (en) | 2012-04-28 | 2012-04-28 | Experiment device for anaerobic microorganism culture |
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| Country | Link |
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| CN (1) | CN202576404U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403986A (en) * | 2014-11-07 | 2015-03-11 | 德州创迪微生物资源有限责任公司 | Microbe spore culture method and equipment |
| CN104450508A (en) * | 2014-12-24 | 2015-03-25 | 于东宁 | Anaerobe inoculating device, anaerobe culturing system and anaerobe inoculation and culture method |
| CN104762191A (en) * | 2014-12-02 | 2015-07-08 | 湖南农业大学 | Economic high-efficiency anacerobe culture apparatus |
| CN106318854A (en) * | 2015-06-26 | 2017-01-11 | 中国石油化工股份有限公司 | Microbial reaction device for observing and sampling conveniently and using method thereof |
-
2012
- 2012-04-28 CN CN 201220193690 patent/CN202576404U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403986A (en) * | 2014-11-07 | 2015-03-11 | 德州创迪微生物资源有限责任公司 | Microbe spore culture method and equipment |
| CN104762191A (en) * | 2014-12-02 | 2015-07-08 | 湖南农业大学 | Economic high-efficiency anacerobe culture apparatus |
| CN104450508A (en) * | 2014-12-24 | 2015-03-25 | 于东宁 | Anaerobe inoculating device, anaerobe culturing system and anaerobe inoculation and culture method |
| CN104450508B (en) * | 2014-12-24 | 2016-01-13 | 于东宁 | Anaerobion classification inoculation apparatus, anaerobion culture systems and anaerobion inoculate, cultural method |
| CN106318854A (en) * | 2015-06-26 | 2017-01-11 | 中国石油化工股份有限公司 | Microbial reaction device for observing and sampling conveniently and using method thereof |
| CN106318854B (en) * | 2015-06-26 | 2018-12-25 | 中国石油化工股份有限公司 | A kind of micro-biological reaction device and application method convenient for observing and sampling |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: SHANGHAI YUANYAO BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: SHANGHAI YUANYAO BIOLOGICAL TECHNOLOGY CO., LTD. |
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| CP03 | Change of name, title or address |
Address after: 201316 Pudong New Area City, the town of Shanghai, the first floor of the barley Bay Industrial Zone, No. 118, No. 5842 Patentee after: SHANGHAI YUANYAO BIOLOGICAL CO., LTD. Address before: 201316 Shanghai city Pudong New Area Navigation Road No. 109 Patentee before: Shanghai Yuanyao Biotechnology Co., Ltd. |
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| CP02 | Change in the address of a patent holder |
Address after: No. 2268, No. 2268, hang Crane Road, Shanghai, Shanghai Patentee after: SHANGHAI YUANYAO BIOLOGICAL CO., LTD. Address before: 201316 Pudong New Area City, the town of Shanghai, the first floor of the barley Bay Industrial Zone, No. 118, No. 5842 Patentee before: SHANGHAI YUANYAO BIOLOGICAL CO., LTD. |
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| CP02 | Change in the address of a patent holder | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121205 Termination date: 20200428 |
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| CF01 | Termination of patent right due to non-payment of annual fee |