CN204251599U - A kind of anaerobic microorganism culturing device - Google Patents
A kind of anaerobic microorganism culturing device Download PDFInfo
- Publication number
- CN204251599U CN204251599U CN201420535899.2U CN201420535899U CN204251599U CN 204251599 U CN204251599 U CN 204251599U CN 201420535899 U CN201420535899 U CN 201420535899U CN 204251599 U CN204251599 U CN 204251599U
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- Prior art keywords
- tank body
- anaerobic
- gas
- tank
- stainless steel
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- 238000012258 culturing Methods 0.000 title claims abstract description 18
- 244000005700 microbiome Species 0.000 title claims abstract description 18
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 29
- 239000010935 stainless steel Substances 0.000 claims description 29
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 231100000033 toxigenic Toxicity 0.000 abstract description 4
- 230000001551 toxigenic effect Effects 0.000 abstract description 4
- 230000003466 anti-cipated effect Effects 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 28
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 3
- 230000002745 absorbent Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 229940123973 Oxygen scavenger Drugs 0.000 description 1
- 239000003637 basic solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000009841 combustion method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to microorganism culturing field, discloses a kind of anaerobic microorganism culturing device, comprises gas tank, tank body and vacuum pump, and described gas tank, built with anaerobic gas, is provided with the plate frame for culture dish holding in described tank body; Described tank body top seal is connected with lid, and described lid is communicated with vacuum pump with gas tank respectively by two airways, and two described airways are all communicated with tank interior, and two described airways are equipped with valve; Described lid is provided with tensimeter.The utility model is connected to vacuum pump and gas tank on tank body, because the anaerobic gas in gas tank was anticipated, and tank body to vacuumize rear inner impurity less, therefore can ensure in tank body, to obtain the anaerobic environment met the demands, ensure the normal growth of anaerobion; Can obtaining by vacuumizing and be filled with anaerobic gas the anaerobic environment met the demands, therefore without the need to using expensive anaerobism bag or anaerobic box, reducing toxigenic capacity.
Description
Technical field
The utility model relates to field of microbial culture technology, particularly a kind of anaerobic microorganism culturing device.
Background technology
The cultivation of anerobe at present laboratory and hospital all more common, conventional Anaerobic culturel way has: candle combustion method, with oxygen scavenger, utilize the methods such as the oxygen uptake reaction of pyrogallol and basic solution to realize obtaining the object of anaerobic environment, aforesaid operations all carries out in anaerobism bag or anaerobic box, anaerobism bag or anaerobic box price more expensive, toxigenic capacity is higher.But the culture condition of anerobe requires strict, and the method for existing acquisition anaerobic environment exists certain defect, anaerobic bacteria culture failure usually can be caused.
Utility model content
(1) technical problem that will solve
The technical problems to be solved in the utility model is: cultivate employing anaerobism bag or anaerobic box acquisition anaerobic environment for solving existing anaerobion, cost is higher, and anaerobism acquisition methods exists certain defect, easily causes the problem of microorganism culturing failure.
(2) technical scheme
In order to solve the problems of the technologies described above, the utility model provides a kind of anaerobic microorganism culturing device, comprises gas tank, tank body and vacuum pump, and described gas tank, built with anaerobic gas, is provided with the plate frame for culture dish holding in described tank body; Described tank body top seal is connected with lid, and described lid is communicated with vacuum pump with gas tank respectively by two airways, and two described airways are all communicated with tank interior, and two described airways are equipped with valve; Described lid is provided with the tensimeter for measuring tank interior pressure.
Wherein, the bottom of described tank body is inverted pyramidal structure, and the bottom of described pyramidal structure is plane; In described tank body, and the top being positioned at described pyramidal structure is provided with dividing plate, and described dividing plate is provided with some apertures, and described dividing plate is transparent resin material; Described plate chord position is on dividing plate.
Wherein, the top of described tank body is connected with stainless steel coil, and described stainless steel coil is provided with sealing-ring; Described lid is stainless steel cover, and described sealing-ring is between stainless steel coil and stainless steel cover.
Further, described stainless steel cover is provided with some bolts, and described stainless steel cover is connected with tank body by bolt.
Wherein, described lid is provided with inlet mouth and air outlet, and described inlet mouth is communicated with gas tank by airway, and described production well is communicated with vacuum pump by airway; Described airway is connected with inlet mouth and air outlet respectively by spiral cover.
Wherein, the airway connecting described gas tank and inlet mouth is rubber hose, and the valve be arranged on described rubber hose is tongs.
Wherein, described inlet mouth place is provided with filtering membrane, for filtering the impurity in anaerobic gas.
(3) beneficial effect
Technique scheme tool has the following advantages: a kind of anaerobic microorganism culturing device of the utility model, tank body is connected to vacuum pump and gas tank, because the anaerobic gas in gas tank was anticipated, and tank body to vacuumize rear inner impurity less, therefore can ensure in tank body, to obtain the anaerobic environment met the demands, ensure the normal growth of anaerobion; Can obtaining by vacuumizing and be filled with anaerobic gas the anaerobic environment met the demands, therefore without the need to using expensive anaerobism bag or anaerobic box, reducing toxigenic capacity.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of anaerobic microorganism culturing device of the utility model.
Wherein, 1, gas tank; 2, valve; 3, spiral cover; 4, tensimeter; 5, vacuum pump; 6, stainless steel cover; 7, sealing-ring; 8, plate frame; 9, tank body; 10, dividing plate.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples for illustration of the utility model, but are not used for limiting scope of the present utility model.
As shown in Figure 1, a kind of anaerobic microorganism culturing device of the utility model, comprising: gas tank 1, vacuum pump 5 and tank body 9, gas tank 1 built with anaerobic gas, such as: nitrogen or carbonic acid gas etc.; Tank body 9 bottom is lower little large conical structure, and the bottom surface of this conical structure is horizontal plane.The top level being positioned at conical structure in tank body 9 is provided with a dividing plate 10, and dividing plate 10 is provided with some apertures, can place siccative or oxygen absorbent below dividing plate 10, to absorb oxygen in tank body 9 or moisture, ensures the growth of anaerobion; Dividing plate 10 is resistant to elevated temperatures transparent resin material.Dividing plate 10 is provided with the plate frame 8 for culture dish holding.Tank body 9 top is opened wide, its opening part is stainless steel coil, stainless steel coil is provided with bolt connecting hole, lid is connected with above tank body 9, lid is stainless steel cover 6, lid is provided with some bolts, is provided with sealing-ring 7 between stainless steel cover 6 and stainless steel coil, and stainless steel cover 6 is tightly connected by bolt and tank body 9.Lid is provided with inlet mouth and air outlet, and inlet mouth is connected with rubber hose by spiral cover 3, and rubber hose is communicated with gas tank 1, and rubber hose is provided with tongs, and inlet mouth place is provided with filtering membrane; Air outlet is connected with airway by spiral cover 3, and airway is communicated with vacuum pump 5, and airway is provided with valve 2; Rubber hose is all connected by spiral cover 3 with production well with inlet mouth, airway, and this device can ensure to dismantle after rubber hose or airway, still can keep original pressure in tank body.Stainless steel cover 6 is also provided with tensimeter 4, and its measuring junction is communicated with, for the air pressure of measuring tube inside with tank body 9 inside.
The principle of work of a kind of anaerobic microorganism culturing device of the utility model is as follows:
Oxygen absorbent or siccative are put in the pyramidal structure bottom tank body 9, then put dividing plate 10 well; Culture dish is placed on after on plate frame 8, then plate frame 8 is placed on dividing plate 10; Sealing-ring 7 circle is placed on tank body 9 opening part, stainless steel cover 6 is covered, is then fastened by bolts, realize being tightly connected of stainless steel cover 6 and tank body 9; Close tongs, open valve 2 and vacuum pump 5, tank body 9 is vacuumized, observe the vacuum tightness that tensimeter 4 shows; When vacuum tightness reaches certain value, valve-off 2, and close vacuum pump 5; Then open tongs, the anaerobic gas in gas tank 1 is filled with in tank body 9, when to show vacuum tightness be zero to tensimeter 4, close tongs; Turn spiral cover 3, disconnect the connection of gas tank 1 and vacuum pump 5 and tank body 9, and keep the sealing of tank body 9 inside, then tank body 9 is placed in incubator, sets culture temperature, can start to cultivate.
Because the anaerobic gas in gas tank 1 was anticipated, it is less that tank body 9 vacuumizes rear inner impurity, therefore can ensure can obtain the anaerobic environment met the demands in tank body 9, ensures the normal growth of anaerobion.Can obtaining by vacuumizing and be filled with anaerobic gas the anaerobic environment met the demands, therefore without the need to using expensive anaerobism bag or anaerobic box, reducing toxigenic capacity.
As can be seen from the above embodiments, anaerobism gas tank 1 is directly communicated with tank body 9 with vacuum pump 5 by pipeline by the utility model, can be made to obtain the anaerobic environment satisfied condition in tank body 9 by opening-closing valve 2 and vacuum pump 5; Anaerobic gas inlet mouth place is provided with filtering membrane, the impurity in the anaerobic gas entering tank body 9 can be filtered, ensure to obtain the anaerobism atmosphere satisfied condition. the connection of gas tank 1 and vacuum pump 5 and tank body 9 can be disconnected by spiral cover 3, directly tank body 9 is placed in incubator, easy to operate, after dismantling vacuum pump 5, connect gas tank 1 again, and then dismantle; Only surplus tank body 9 and stainless steel cover 6 after dismounting gas tank 1 and vacuum pump 5, volume is little, takes up room little, is convenient to carrying; Be provided with dividing plate 10 bottom tank body 9, below dividing plate 10, oxygen absorbent, oxygen indicator or siccative can be placed, to obtain better anaerobic environment; At the edge of tank body 9 opening part, one circle stainless steel coil is set, then by bolt, stainless steel cover 6 is connected with stainless steel coil, and be lined with sealing-ring 7 between the two, thus ensure being tightly connected of stainless steel cover 6 and tank body 9, ensure to form independently seal cavity in tank body 9.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite not departing from the utility model know-why; can also make some improvement and replacement, these improve and replace and also should be considered as protection domain of the present utility model.
Claims (7)
1. an anaerobic microorganism culturing device, it is characterized in that, comprise gas tank (1), tank body (9) and vacuum pump (5), described gas tank (1), built with anaerobic gas, is provided with the plate frame (8) for culture dish holding in described tank body (9); Described tank body (9) top seal is connected with lid, described lid is communicated with vacuum pump (5) with gas tank (1) respectively by two airways, two described airways are all communicated with tank body (9) inside, two described airways are equipped with valve (2); Described lid is provided with the tensimeter (4) for measuring tank body (9) internal pressure.
2. anaerobic microorganism culturing device as claimed in claim 1, it is characterized in that, the bottom of described tank body (9) is inverted pyramidal structure, and the bottom of described pyramidal structure is plane; In described tank body (9), and the top being positioned at described pyramidal structure is provided with dividing plate (10), and described dividing plate (10) is provided with some apertures, and described dividing plate (10) is transparent resin material; Described plate frame (8) is positioned on dividing plate (10).
3. anaerobic microorganism culturing device as claimed in claim 1, it is characterized in that, the top of described tank body (9) is connected with stainless steel coil, and described stainless steel coil is provided with sealing-ring (7); Described lid is stainless steel cover (6), and described sealing-ring (7) is positioned between stainless steel coil and stainless steel cover (6).
4. anaerobic microorganism culturing device as claimed in claim 3, it is characterized in that, described stainless steel cover (6) is provided with some bolts, and described stainless steel cover (6) is connected with tank body (9) by bolt.
5. anaerobic microorganism culturing device as claimed in claim 1, it is characterized in that, described lid is provided with inlet mouth and air outlet, and described inlet mouth is communicated with gas tank (1) by airway, and described production well is communicated with vacuum pump (5) by airway; Described airway is connected with inlet mouth and air outlet respectively by spiral cover (3).
6. anaerobic microorganism culturing device as claimed in claim 5, is characterized in that, connecting described gas tank (1) with the airway of inlet mouth is rubber hose, and the valve (2) be arranged on described rubber hose is tongs.
7. anaerobic microorganism culturing device as claimed in claim 5, it is characterized in that, described inlet mouth place is provided with filtering membrane, for filtering the impurity in anaerobic gas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420535899.2U CN204251599U (en) | 2014-09-17 | 2014-09-17 | A kind of anaerobic microorganism culturing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420535899.2U CN204251599U (en) | 2014-09-17 | 2014-09-17 | A kind of anaerobic microorganism culturing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204251599U true CN204251599U (en) | 2015-04-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420535899.2U Expired - Lifetime CN204251599U (en) | 2014-09-17 | 2014-09-17 | A kind of anaerobic microorganism culturing device |
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| Country | Link |
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| CN (1) | CN204251599U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN106281998A (en) * | 2015-05-29 | 2017-01-04 | 复旦大学附属华山医院 | A kind of external dynamic pharmacokinetics/pharmacodynamics model system of anaerobe and construction method |
| CN107227253A (en) * | 2017-07-26 | 2017-10-03 | 中国农业科学院兰州兽医研究所 | A kind of culture device for anaerobic bacteria and cultural method |
| CN116694448A (en) * | 2023-05-09 | 2023-09-05 | 中国科学院东北地理与农业生态研究所农业技术中心 | Convenient preservation system of many bacterial |
-
2014
- 2014-09-17 CN CN201420535899.2U patent/CN204251599U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106281998A (en) * | 2015-05-29 | 2017-01-04 | 复旦大学附属华山医院 | A kind of external dynamic pharmacokinetics/pharmacodynamics model system of anaerobe and construction method |
| CN106281998B (en) * | 2015-05-29 | 2020-04-24 | 复旦大学附属华山医院 | Anaerobic in-vitro dynamic pharmacokinetics/pharmacodynamics model system and construction method |
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN107227253A (en) * | 2017-07-26 | 2017-10-03 | 中国农业科学院兰州兽医研究所 | A kind of culture device for anaerobic bacteria and cultural method |
| CN116694448A (en) * | 2023-05-09 | 2023-09-05 | 中国科学院东北地理与农业生态研究所农业技术中心 | Convenient preservation system of many bacterial |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: CHENGDU HUALUO BIOTECHNOLOGY Co.,Ltd. Assignor: CHINA ANIMAL HUSBANDRY INDUSTRY Co.,Ltd. Contract record no.: 2016990000446 Denomination of utility model: Anaerobic microorganism culture device Granted publication date: 20150408 License type: Common License Record date: 20161026 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CX01 | Expiry of patent term |
Granted publication date: 20150408 |