CN205275598U - Novel anaerobe culture ware - Google Patents
Novel anaerobe culture ware Download PDFInfo
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- CN205275598U CN205275598U CN201521075774.7U CN201521075774U CN205275598U CN 205275598 U CN205275598 U CN 205275598U CN 201521075774 U CN201521075774 U CN 201521075774U CN 205275598 U CN205275598 U CN 205275598U
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- culture dish
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- gate valve
- dish body
- air inlet
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Abstract
The utility model discloses a novel anaerobe culture ware, including culture dish body and lid, the culture dish body passes through threaded connection rather than the lid of top, forms inclosed cavity. The one side at lid top is provided with the intake pipe, disposes gas filter in the intake pipe, still disposes first gate valve in the intake pipe, first gate valve is located between gas filter and the lid. The opposite side at lid top is provided with the outlet duct, has the observation chamber that is used for placing the oxygen indicator on the outlet duct, be provided with the second gate valve on the outlet duct between observation chamber and the lid. The utility model discloses simple structure, the practicality is strong, low in manufacturing cost, easily the operation is convenient for extensively use widely.
Description
Technical field
This utility model relates to geomicrobiology experimental implementation field, is specifically related to a kind of Novel anaerobic bacterium culture dish.
Background technology
Culture dish, is generally used for the cultivation of animal and plant cells or microbial cell, is often made up of glass or plastics, simple in construction. Technical improvement about culture dish is more, except conventional can except the glass culture dish that possess different-diameter of sterilizing, the culture dish of one more. But, these culture dishs mostly utilize aerobic environment to carry out cell cultivation. It is specifically designed to the culture dish cultivating anaerobe at present less.
Cultivate anaerobe, it is necessary to creating the environment of an anaerobic, some anaerobe even needs strict oxygen-free environment. Typically by culture medium adds reducing agent, or remove the free oxygen in environment by physics, chemical method, to reduce redox potential. Conventional anaerobic culture method has many, for instance the methods such as layer plating method, anaerobism work box culture method, Anaerobicbag, biological oxygen consumption. Above-mentioned cultural method complicated operation, some method anaerobism effect is undesirable. In order to cultivate anaerobe more easily, it is necessary to make a kind of culture dish being specifically designed to and cultivating anaerobe, it is desirable to this culture dish has good obturation effect, creating a kind of anaerobic environment, incubation is simply easily operated simultaneously.
Utility model content
The features such as above-mentioned the deficiencies in the prior art, this utility model provides a kind of Novel anaerobic bacterium culture dish, and this culture dish possesses good sealing effect, is provided that anaerobic environment, simple to operate.
In order to solve above-mentioned technical problem, this utility model be the technical scheme is that
A kind of Novel anaerobic bacterium culture dish, is threaded connection including culture dish body and lid, described culture dish body and the lid above it, forms airtight cavity. The side at described lid top is provided with air inlet pipe, and air inlet pipe is configured with pneumatic filter, and air inlet pipe is also configured with the first gate valve, and described first gate valve is between pneumatic filter and lid. The opposite side at described lid top is provided with escape pipe, escape pipe has the spherical observation chamber for placing oxygen indicator, the escape pipe between described spherical observation chamber and lid is provided with the second gate valve.
Preferably, one end of described air inlet pipe communicates with the inside of culture dish body, and the other end can connect nitrogen gas cylinder. One end of described escape pipe also communicates with the inside of culture dish body, and the other end communicates with the external world.
Preferably, described pneumatic filter is the bilayer filter with membrane filtration layer and activated carbon.
Preferably, the lateral wall of described culture dish body open end being provided with external screw thread, the medial wall of lid opening is provided with female thread, external screw thread is suitable with female thread.
Preferably, the medial wall of described lid being provided with the mounting seat of annular, mounting seat is configured with rubber seal near the side of female thread.
By adopting technique scheme, Advantageous Effects of the present utility model is: owing to external screw thread and female thread are suitable, and sealing ring is set in lid, can ensure that culture dish obturation effect, being filled with nitrogen through air inlet pipe, original gas in culture dish discharged by exhaustor, makes the oxygen content in culture dish decline, changed by the color of the oxygen indicator of spherical observation intracavity, it is possible to illustrate whether the oxygen content in culture dish has reached the requirement of anaerobe growth. This utility model simple in construction, practical, low cost of manufacture, it is easy to operation, it is simple to be widely popularized use.
Accompanying drawing explanation
Fig. 1 is the structural principle schematic diagram of a kind of Novel anaerobic bacterium culture dish of this utility model.
Detailed description of the invention
Below in conjunction with accompanying drawing, this utility model is described in detail:
In conjunction with Fig. 1, a kind of Novel anaerobic bacterium culture dish, including culture dish body 1 and lid 2, described culture dish body 1 and the lid 2 above it are threaded connection, and form airtight cavity. Being provided with external screw thread 3 on the lateral wall of described culture dish body 1 opening, the medial wall of lid 2 opening is provided with female thread 4, described external screw thread 3 is suitable with female thread 4. Being provided with the mounting seat 5 of annular on the medial wall of described lid 1, mounting seat 5 is configured with rubber seal 6 near the side of female thread 4. The side at described lid 2 top is provided with air inlet pipe 7, and one end of air inlet pipe 7 communicates with the inside of culture dish body 1, and the other end can connect nitrogen gas cylinder. Being configured with pneumatic filter 8 in air inlet pipe 7, described pneumatic filter 8 preferably has the bilayer filter of membrane filtration layer and activated carbon.
Being also configured with the first gate valve 9 in air inlet pipe 7, the first gate valve 9 controls nitrogen and enters culture dish body 1 and the closed cavity of lid 2 formation through air inlet pipe 7, and described first gate valve 9 is between pneumatic filter 8 and lid 2. The opposite side at described lid 2 top is provided with escape pipe 10, and one end of escape pipe 10 also communicates with the inside of culture dish body 1, and the other end communicates with the external world. Escape pipe 10 has the spherical observation chamber 11 for placing oxygen indicator, escape pipe between described spherical observation chamber 11 and lid 2 is provided with the second gate valve 12, described second gate valve 12 controls the gas in the closed cavity of culture dish body 1 and lid 2 formation, is discharged to the external world via escape pipe 10.
First, first gate valve 9, pneumatic filter 8 are fitted together with air inlet pipe 7, second gate valve 12 is fitted together with the escape pipe 10 with spherical observation chamber 11, air inlet pipe 7 and escape pipe 10 are connected respectively to the top of lid 2 again, meanwhile, in spherical observation chamber 11, oxygen indicator is added. anaerobe is placed in culture dish body 1, lid 2 and culture dish body 1 are tightened sealing, open the first gate valve 9, nitrogen after pneumatic filter 8 filters enters in culture dish body 1, open the second gate valve 12 simultaneously, mixing gas in culture dish body 1, it is discharged to the external world through escape pipe 10, in its process, culture dish body 1 oxygen is gradually lowered, when oxygen indicator becomes peach time, oxygen content in culture dish body 1 has dropped to of a sufficiently low level, the growth of anaerobe can be met, simultaneously close off the first gate valve 9 and the second gate valve 12, culture dish body 1 is kept to seal.
Certainly; described above is not to restriction of the present utility model; this utility model is also not limited to the example above, the change made in essential scope of the present utility model of those skilled in the art, remodeling, interpolation or replacement, also should belong to protection domain of the present utility model.
Claims (5)
1. a Novel anaerobic bacterium culture dish, including culture dish body and lid, it is characterised in that described culture dish body and the lid above it are threaded connection, and form airtight cavity; The side at described lid top is provided with air inlet pipe, and air inlet pipe is configured with pneumatic filter, and air inlet pipe is also configured with the first gate valve, and described first gate valve is between pneumatic filter and lid; The opposite side at described lid top is provided with escape pipe, escape pipe has the spherical observation chamber for placing oxygen indicator, the escape pipe between described spherical observation chamber and lid is provided with the second gate valve.
2. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterised in that one end of described air inlet pipe communicates with the inside of culture dish body, and the other end can connect nitrogen cylinder; One end of described escape pipe also communicates with the inside of culture dish body, and the other end communicates with the external world.
3. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterised in that described pneumatic filter is the bilayer filter with membrane filtration layer and activated carbon.
4. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterised in that being provided with external screw thread on the lateral wall of described culture dish body open end, the medial wall of lid opening is provided with female thread, external screw thread is suitable with female thread.
5. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterised in that being provided with the mounting seat of annular on the medial wall of described lid, mounting seat is configured with rubber seal near the side of female thread.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521075774.7U CN205275598U (en) | 2015-12-21 | 2015-12-21 | Novel anaerobe culture ware |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521075774.7U CN205275598U (en) | 2015-12-21 | 2015-12-21 | Novel anaerobe culture ware |
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| Publication Number | Publication Date |
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| CN205275598U true CN205275598U (en) | 2016-06-01 |
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| CN201521075774.7U Active CN205275598U (en) | 2015-12-21 | 2015-12-21 | Novel anaerobe culture ware |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN106442066A (en) * | 2016-11-09 | 2017-02-22 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small air collector |
| CN106987515A (en) * | 2017-05-10 | 2017-07-28 | 河海大学 | Free-assembling multichannel degradation of pesticide bacterial strain screening plant |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
-
2015
- 2015-12-21 CN CN201521075774.7U patent/CN205275598U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385582A (en) * | 2015-12-21 | 2016-03-09 | 山东科技大学 | Novel anaerobic-bacterium culturing dish |
| CN106442066A (en) * | 2016-11-09 | 2017-02-22 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small air collector |
| CN106442066B (en) * | 2016-11-09 | 2023-10-13 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small gas collector |
| CN106987515A (en) * | 2017-05-10 | 2017-07-28 | 河海大学 | Free-assembling multichannel degradation of pesticide bacterial strain screening plant |
| CN106987515B (en) * | 2017-05-10 | 2019-06-21 | 河海大学 | Free-assembling multichannel degradation of pesticide bacterial strain screening plant |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN109022256B (en) * | 2018-07-17 | 2022-05-13 | 广东药科大学附属第一医院 | Anaerobic culture bag with anaerobic indicator |
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