CN111961579B - Microbial culture medium and culture method thereof - Google Patents
Microbial culture medium and culture method thereof Download PDFInfo
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- CN111961579B CN111961579B CN202011138706.6A CN202011138706A CN111961579B CN 111961579 B CN111961579 B CN 111961579B CN 202011138706 A CN202011138706 A CN 202011138706A CN 111961579 B CN111961579 B CN 111961579B
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a microbial culture medium and a culture method thereof, wherein the microbial culture medium comprises a culture medium body, an installation seat is arranged on the inner bottom wall of the culture medium body in a sliding mode, heating pipes are fixedly matched with the lower portions of two sides of the inner wall of the installation seat, a placing box is arranged right above the heating pipes and in threaded connection with the installation seat through threaded rods, two transverse electric sliding tables are assembled on the inner top wall of the culture medium body, longitudinal electric sliding tables are connected to the sliding ends of the two transverse electric sliding tables in a matching mode, a culture solution spray head is installed on the sliding end of each longitudinal electric sliding table, a hose is fixedly connected to the top end of each culture solution spray head, a three-way pipe is assembled at the upper end of each hose, a liquid outlet pipe and an oxygen pipe are respectively connected to two ends of each. The invention has high culture efficiency, saves the artificial culture time, solves the problem of insufficient oxygen in the microorganisms in the culture process, has reasonable design and simple operation, and is suitable for popularization.
Description
Technical Field
The invention relates to the technical field of microbial culture, in particular to a microbial culture medium and a culture method thereof.
Background
The general name of all micro-organisms which are difficult to be seen by naked eyes and can be observed by an optical microscope or an electron microscope. Microorganisms include bacteria, viruses, fungi, and a few algae, among others. (some microorganisms are visible to the naked eye, like mushrooms, ganoderma, etc., which belong to fungi.) viruses are a class of "non-cellular organisms" consisting of a few components, such as nucleic acids and proteins, but whose survival must be dependent on living cells. The microorganism is classified into space microorganism, marine microorganism and the like according to different existing environments, and is classified into prokaryotic microorganism and eukaryotic microorganism according to cell structures.
At present, when a culture medium is used for culturing microorganisms in food, a large amount of waste gas is generated, so that oxygen in the culture medium is insufficient, meanwhile, the waste gas in the culture medium is difficult to discharge, the culture quality of the microorganisms is influenced, and the existing culture medium is uneven in distribution when a culture solution and oxygen are added, so that a large amount of culture time is consumed, and the culture requirement cannot be met.
Disclosure of Invention
The invention aims to solve the problems that a large amount of waste gas is generated when microorganisms in food are cultured so as to cause insufficient oxygen in a culture medium and the waste gas in the culture medium is difficult to discharge in the prior art, and provides a microorganism culture medium and a culture method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microbial culture medium comprises a culture medium body, wherein a mounting seat is slidably arranged on the inner bottom wall of the culture medium body, heating pipes are fixedly matched with the lower portions of two sides of the inner wall of the mounting seat body, a placing box is arranged right above the heating pipes and is in threaded connection with the mounting seat body through a threaded rod, two transverse electric sliding tables are assembled on the inner top wall of the culture medium body, longitudinal electric sliding tables are connected to the sliding ends of the two transverse electric sliding tables in a matched mode, a culture solution sprayer is installed on the sliding end of each longitudinal electric sliding table, a hose is fixedly connected to the top end of the culture solution sprayer, a three-way pipe is assembled at the upper end of each hose, a liquid outlet pipe and an oxygen pipe are respectively connected to two ends of each three-way pipe, an oxygen bottle is fixedly installed at one end of each oxygen pipe, a circulating pump is connected to one end of each liquid outlet pipe, a culture, the one end of oxygen hose is equipped with second valve and third valve respectively, the outer wall one side of culture medium is fixed with the exhaust gas pipeline, and the exhaust gas rose box is installed to the one end of exhaust gas pipeline, be equipped with filter screen and adsorption net on the inner wall of exhaust gas rose box respectively, be equipped with the fourth valve on the exhaust gas pipeline.
Preferably, four corners of the inner bottom wall of the culture medium are respectively fixed with a sliding sleeve, the sliding sleeve is connected with a sliding block in a sliding manner, and the top of the sliding block is fixed with the bottom of the mounting seat.
Preferably, the both sides of placing the case all rotate and install the threaded rod, and the top of mount pad is equipped with the screw hole, the lower extreme threaded connection of threaded rod is in the inside of screw hole.
Preferably, the culture solution spray head is externally connected with an annular plate, the bottom of the annular plate is provided with an ultraviolet lamp, and the ultraviolet lamp is in an annular structure.
Preferably, the top of culture medium is equipped with the connecting hole, and the upper end of hose runs through the connecting hole and extends to the outside of culture medium, the hose sets up with the connecting hole is sealed.
Preferably, the two sides of the outer wall of the front side of the culture medium are hermetically connected with a box door, the front side of the box door is provided with a handle, and the box door is made of transparent PVC materials.
Preferably, the lower end of the culture solution spray head extends to the lower part of the ultraviolet lamp and is positioned above the placing box.
Preferably, one side of the inner top wall of the culture solution box is connected with a liquid feeding pipe, and the upper end of the liquid feeding pipe is in threaded connection with a pipe cover.
A method of culturing a microorganism, said method comprising:
the saccharomycete cultured in a high-salt environment sample source is used as a saccharomycete source, a sugar-containing organic matter is used as a main carbon source, the saccharomycete reacts in a culture medium, the salt concentration, the pH value and the temperature are controlled, and the saccharomycete flora suitable for salt in the high-salt environment sample is mixed and co-cultured into the microorganism in a continuous or intermittent mode.
Compared with the prior art, the invention provides a microbial culture medium and a preparation method thereof, and the microbial culture medium has the following beneficial effects:
1. through being provided with place case, first valve, culture solution case, circulating pump, drain pipe, three-way pipe, hose, culture solution shower nozzle, horizontal electronic slip table and vertical electronic slip table, can spray the culture solution on food uniformly for food fully contacts with the culture solution, is convenient for cultivate the microorganism, and the cooperation has the heating pipe, temperature when can changing the microorganism and cultivate, makes the microorganism cultivate under suitable temperature, has improved the cultivation effect.
2. Through being provided with culture medium, first valve, second valve, third valve, oxygen cylinder, oxygen hose and hose for oxygen distribution has increased the distribution range of oxygen in the different positions of culture medium, the not enough condition of oxygen appears when avoiding the microorganism to cultivate, is favorable to promoting the growth of microorganism, and the cooperation has fourth valve, exhaust gas pipeline, waste gas rose box, filter screen and adsorption net, and the waste gas discharge in the culture medium of being convenient for can not lead to the fact the pollution to external environment.
The invention has high culture efficiency, saves the artificial culture time, solves the problem of insufficient oxygen in the microorganisms in the culture process, has reasonable design and simple operation, and is suitable for popularization.
Drawings
FIG. 1 is a schematic sectional view of a microorganism culture medium according to the present invention;
FIG. 2 is a schematic front view of a microorganism culture medium according to the present invention;
FIG. 3 is a schematic view of a partial enlarged structure at A in a microorganism culture medium according to the present invention;
FIG. 4 is a schematic cross-sectional view of an exhaust gas filtering box in a microorganism culture medium according to the present invention;
FIG. 5 is a schematic top view of a housing box and threaded rod in a microbiological culture medium according to the invention;
FIG. 6 is a schematic top view of the electric sliding table in the transverse direction and the electric sliding table in the longitudinal direction in the microorganism culture medium according to the present invention.
In the figure: 1. a culture medium; 2. a sliding sleeve; 3. a slider; 4. a mounting seat; 5. heating a tube; 6. placing a box; 7. a threaded rod; 8. a transverse electric sliding table; 9. a longitudinal electric sliding table; 10. a culture solution spray head; 11. an annular plate; 12. an ultraviolet lamp; 13. a hose; 14. a three-way pipe; 15. a culture solution box; 16. a circulation pump; 17. a liquid outlet pipe; 18. an oxygen cylinder; 19. an oxygen tube; 20. a liquid feeding pipe; 21. an exhaust gas conduit; 22. an exhaust gas filter tank; 23. a filter screen; 24. an adsorption net; 25. and (4) a box door.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-6, a microbial cultivation medium comprises a cultivation medium 1, a mounting seat 4 is slidably disposed on an inner bottom wall of the cultivation medium 1, a heating pipe 5 is fixedly matched with lower portions of two sides of an inner wall of the mounting seat 4, a placing box 6 is disposed right above the heating pipe 5, the placing box 6 is in threaded connection with the mounting seat 4 through a threaded rod 7, two transverse electric sliding tables 8 are assembled on an inner top wall of the cultivation medium 1, a longitudinal electric sliding table 9 is connected on sliding ends of the two transverse electric sliding tables 8 in a matched manner, a cultivation liquid nozzle 10 is mounted on a sliding end of the longitudinal electric sliding table 9, a hose 13 is fixedly connected with a top end of the cultivation liquid nozzle 10, a three-way pipe 14 is assembled on an upper end of the hose 13, a liquid outlet pipe 17 and an oxygen pipe 19 are respectively connected with two ends of the three-way pipe 14, an oxygen bottle 18 is fixedly, the outside of circulating pump 16 is provided with culture solution box 15, is equipped with first valve on drain pipe 17, and the one end of oxygen hose 19 is equipped with second valve and third valve respectively, and the outer wall one side of culture medium 1 is fixed with exhaust gas pipeline 21, and exhaust gas filter box 22 is installed to exhaust gas pipeline 21's one end, is equipped with filter screen 23 and adsorption net 24 on exhaust gas filter box 22's the inner wall respectively, is equipped with the fourth valve on the exhaust gas pipeline 21.
In the embodiment, four corners of the inner bottom wall of the culture medium 1 are respectively fixed with a sliding sleeve 2, the sliding sleeve 2 is connected with a sliding block 3 in a sliding manner, and the top of the sliding block 3 is fixed with the bottom of an installation seat 4; through pulling mount pad 4 for 4 sliders 3 of mount pad slide out from the inside of sliding sleeve 2, be convenient for shift out the outside of culture medium 1 with mount pad 4, simple to operate.
In the embodiment, threaded rods 7 are rotatably mounted on two sides of the placing box 6, a threaded hole is formed in the top of the mounting seat 4, and the lower end of each threaded rod 7 is connected to the inside of the corresponding threaded hole in a threaded manner; move out the outside back of culture medium 1 with mount pad 4, rotate threaded rod 7 for threaded rod 7 and screw hole phase separation, thereby realized installing and dismantling placing case 6, the equipment is convenient.
In this embodiment, the exterior of the culture solution nozzle 10 is connected with an annular plate 11, the bottom of the annular plate 11 is equipped with an ultraviolet lamp 12, and the ultraviolet lamp 12 is in an annular structure; by starting the ultraviolet lamp 12, the inside of the culture medium 1 is sterilized, and the microorganism is prevented from being polluted.
In this embodiment, a connection hole is formed in the top of the culture medium 1, the upper end of the flexible tube 13 extends to the outside of the culture medium 1 through the connection hole, and the flexible tube 13 and the connection hole are hermetically arranged; the hose 13 has a certain length and elasticity.
In this embodiment, two sides of the outer wall of the front side of the culture medium 1 are hermetically connected with a box door 25, the front side of the box door 25 is provided with a handle, and the box door 25 is made of transparent PVC material; the box door 25 facilitates observation of the culture condition in the culture medium 1.
In this embodiment, the lower end of the culture solution nozzle 10 extends below the ultraviolet lamp 12 and is located above the placing case 6.
In this embodiment, one side of the inner top wall of the culture solution tank 15 is connected with a liquid feeding pipe 20, and the upper end of the liquid feeding pipe 20 is in threaded connection with a pipe cover; when the culture solution is insufficient, the inside of the culture solution tank 15 is replenished through the charging tube 20.
A method of culturing a microorganism, said method comprising:
the saccharomycete cultured in a high-salt environment sample source is used as a saccharomycete source, a sugar-containing organic matter is used as a main carbon source, the saccharomycete reacts in a culture medium, the salt concentration, the pH value and the temperature are controlled, and the saccharomycete flora suitable for salt in the high-salt environment sample is mixed and co-cultured into the microorganism in a continuous or intermittent mode.
When the device is used, firstly, food to be cultured is placed in the placing box 6, the first valve is opened, the culture solution in the culture solution box 15 enters the food in the placing box 6 through the liquid outlet pipe 17, the three-way pipe 14, the hose 13 and the culture solution spray head 10, the transverse electric sliding table 8 and the longitudinal electric sliding table 9 are started to work, the culture solution spray head 10 moves forwards and backwards and leftwards, the culture solution is uniformly sprayed on the food, the food is fully contacted with the culture solution, the microorganism is conveniently cultured, meanwhile, the temperature during microorganism culture can be changed by starting the heating pipe 5 to work, the microorganism is cultured at a proper temperature, the culture effect is improved, when the oxygen in the culture medium 1 is insufficient, the first valve is closed, the second valve and the third valve are opened, the oxygen in the oxygen bottle 18 enters the culture medium 1 through the oxygen pipe 19 and the hose 13, the cooperation has horizontal electronic slip table 8 and vertical electronic slip table 9 simultaneously, make oxygen distribution in the different positions of culture medium 1, the distribution range of oxygen has been increased, make microorganism and oxygen fully contact, the condition that oxygen is not enough appears when avoiding the microorganism to cultivate, be favorable to promoting the growth of microorganism, open the fourth valve, waste gas in the culture medium 1 passes through exhaust gas conduit 21 and enters into the inside of exhaust gas filter box 22, and discharge after filtering through filter screen 23 and adsorption net 24, can not cause the pollution to the external environment, before and after the cultivation, work through starting ultraviolet lamp 12, disinfect the inside of culture medium 1, avoid causing the pollution to the microorganism, the cultivation is efficient, the artificial cultivation's time has been practiced thrift, and reasonable design, and easy operation is suitable for promoting.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The utility model provides a microorganism culture device, includes culture device main part (1), its characterized in that, it is provided with mount pad (4) to slide on the interior diapire of culture device main part (1), the inner wall both sides lower part fixed coordination of mount pad (4) has heating pipe (5), be provided with directly over heating pipe (5) and place case (6), place case (6) and mount pad (4) threaded connection through threaded rod (7), be equipped with two horizontal electronic slip tables (8) on the interior roof of culture device main part (1), the cooperation is connected with vertical electronic slip table (9) on the slip end of two horizontal electronic slip tables (8), install culture solution shower nozzle (10) on the slip end of vertical electronic slip table (9), the top fixedly connected with hose (13) of culture solution shower nozzle (10), the upper end of hose (13) is equipped with three-way pipe (14), the both ends of three-way pipe (14) are connected with drain pipe (17) and oxygen hose (19) respectively, the one end fixed mounting of oxygen hose (19) has oxygen cylinder (18), the one end of drain pipe (17) is connected with circulating pump (16), the outside of circulating pump (16) is provided with culture solution box (15), be equipped with first valve on drain pipe (17), the one end of oxygen hose (19) is equipped with second valve and third valve respectively, outer wall one side of culture apparatus main part (1) is fixed with exhaust gas pipeline (21), and exhaust gas rose box (22) are installed to the one end of exhaust gas pipeline (21), be equipped with filter screen (23) and adsorption net (24) on the inner wall of exhaust gas rose box (22) respectively, be equipped with the fourth valve on exhaust gas pipeline (21).
2. The microorganism culture device according to claim 1, wherein a sliding sleeve (2) is fixed at each of four corners of the inner bottom wall of the culture device body (1), a sliding block (3) is connected inside the sliding sleeve (2) in a sliding manner, and the top of the sliding block (3) is fixed with the bottom of the mounting seat (4).
3. The microorganism culture device according to claim 1, wherein the placing box (6) is rotatably provided with a threaded rod (7) on both sides, the top of the mounting seat (4) is provided with a threaded hole, and the lower end of the threaded rod (7) is screwed inside the threaded hole.
4. A microorganism culture apparatus according to claim 1, characterized in that the culture solution nozzle (10) is externally connected with an annular plate (11), the bottom of the annular plate (11) is equipped with an ultraviolet lamp (12), and the ultraviolet lamp (12) is in an annular structure.
5. The microorganism culture device according to claim 1, wherein the top of the culture device body (1) is provided with a connection hole, the upper end of the flexible pipe (13) extends to the outside of the culture device body (1) through the connection hole, and the flexible pipe (13) is hermetically arranged with the connection hole.
6. The microorganism culture device according to claim 1, wherein the door (25) is hermetically connected to two sides of the outer wall of the front surface of the culture device main body (1), the handle is assembled on the front surface of the door (25), and the door (25) is made of transparent PVC.
7. A microorganism culture apparatus according to claim 4, wherein the lower end of the culture solution nozzle (10) extends below the ultraviolet lamp (12) and above the placing box (6).
8. The microorganism cultivation apparatus according to claim 1, wherein a filler tube (20) is connected to one side of the inner top wall of the culture solution tank (15), and a tube cap is threadedly connected to the upper end of the filler tube (20).
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| CN202011138706.6A CN111961579B (en) | 2020-10-22 | 2020-10-22 | Microbial culture medium and culture method thereof |
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| CN112522095A (en) * | 2020-12-04 | 2021-03-19 | 李兴 | Medical treatment is with little biological culture device |
| CN113790386A (en) * | 2021-08-10 | 2021-12-14 | 郝花花 | Heating oxygen therapy device for microbial cultivation experiment |
| CN113699022B (en) * | 2021-08-16 | 2024-01-26 | 上海知楚仪器有限公司 | Microorganism culture device and culture method thereof |
| CN114107037B (en) * | 2021-12-06 | 2024-03-08 | 广东轻工职业技术学院 | Automatic temperature-control dendrobium candidum endophytic fungi equipment |
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| CN206005272U (en) * | 2016-08-14 | 2017-03-15 | 杨凌普兆农业科技有限公司 | A kind of moveable mushroom and vegetable culture device |
| CN206923437U (en) * | 2017-07-11 | 2018-01-26 | 李杨名 | Cultivating seeds equipment |
| CN207552298U (en) * | 2017-11-27 | 2018-06-29 | 绍兴百奥尼科技有限公司 | Microorganism detection sensor based on antibacterial peptide |
| CN108812086A (en) * | 2018-08-10 | 2018-11-16 | 河南科技大学 | A kind of agaricus bisporus picker |
| CN209923321U (en) * | 2019-04-23 | 2020-01-10 | 吉林工程职业学院 | Microbial cultivation case for bioengineering |
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