CN210856157U

CN210856157U - Cell biology experiment sample culture equipment

Info

Publication number: CN210856157U
Application number: CN201921832013.XU
Authority: CN
Inventors: 夏继波
Original assignee: Suqian Airuisi Biotechnology Co ltd
Current assignee: Suqian Airuisi Biotechnology Co ltd
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-06-26
Anticipated expiration: 2029-10-29

Abstract

The utility model provides a cell biology experiment sample culture equipment, including the incubator, the incubator bottom is equipped with places the platform, it is equipped with a plurality of observation holes to place the bench, observe hole top both sides fixed mounting clamping device, be equipped with the culture dish in the clamping device, it is connected through pivot and incubator bottom rotation to place the platform bottom, fixed mounting motor in the middle of the incubator bottom, incubator bottom left side fixed mounting camera, incubator outer wall fixed mounting water purifier, incubator left side inner wall fixed mounting heating pipe, fixed mounting detector in the middle of the incubator top inner wall, the incubator top inner wall fixed mounting LED lamp plate of detector both sides, incubator top outer wall fixed mounting air purifier, air purifier's top fixed mounting carbon dioxide gas cylinder. The utility model discloses the sample culture equipment can temperature and carbon dioxide concentration in the automatically regulated incubator, and need not take out the culture dish and can add water to the culture dish, and the camera setting is in the convenient observation monitoring of culture dish bottom.

Description

Cell biology experiment sample culture equipment

Technical Field

The utility model relates to a cell biology experimental facilities field, in particular to cell biology experimental sample cultivates equipment.

Background

Cell Biology (Cell Biology) is a science that studies the structure, function and various life laws of cells at the microscopic, sub-microscopic and molecular levels. Cell biology has evolved from Cytology, which is a study of cellular structure and function (particularly chromosomes). Modern cell biology studies the structure, function and vital activities of cells at different levels, including microscopic, ultramicro and molecular levels. The culture of samples, especially the culture of cells, is an important and common technique in cell biology research methods, and a large number of cells can be obtained through the cell culture, and the signal transduction, the anabolism, the growth and the proliferation of the cells and the like of the cells can be researched.

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. The cell culture includes animal cell culture, plant cell culture and microbial cell culture, wherein most of microbes are unicellular organisms, the wild survival conditions are simpler, so that the conditions for artificially culturing the microbes are much simpler than those for animal and plant cells, and the animal cell culture and the plant cell culture need special conditions, such as a sterile environment, a proper temperature, a proper osmotic pressure, a gas environment, pH and the like. Therefore, cell culture usually needs to use special culture equipment, the existing cell biology experiment sample culture equipment has single function, cannot accurately regulate and control temperature and gas environment, cannot be taken out of a culture dish and added with water during cell culture to enable the culture dish to be easily contacted with bacteria, and cannot meet the requirements of cell biology experiments.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a cell biology experiment sample culture apparatus has solved current culture apparatus function singleness, can not regulate and control temperature, gaseous environment accuracy and inconvenient watered problem to the culture dish.

The utility model provides a sample culture device for cell biology experiments, which comprises an incubator, wherein the bottom of the incubator is provided with a placing platform, the placing platform is provided with a plurality of observation holes, an organic glass plate is fixedly installed in the observation holes, clamping devices are fixedly installed on two sides above the observation holes, culture dishes are arranged in the clamping devices, the bottom of the culture dishes is matched with the observation holes, the bottom of the placing platform is rotatably connected with the bottom of the incubator through a rotating shaft, a motor is fixedly installed in the middle of the bottom of the incubator, the output shaft of the motor is fixedly connected with the rotating shaft of the placing platform, a camera is fixedly installed on the left side of the bottom of the incubator and is positioned under the observation holes, a water purifier is fixedly installed on the outer wall of the incubator, the water outlet end of the water purifier is connected with a liquid feeding pipe, the liquid feeding pipe is fixedly installed on the inner wall on the right side of the, fixed mounting detector in the middle of the incubator top inner wall, the incubator top inner wall fixed mounting LED lamp plate of detector both sides, incubator top outer wall fixed mounting air purifier, incubator rear side inner wall is equipped with a plurality of air inlets, incubator top both sides are equipped with the gas outlet, air purifier's inlet end is connected with the gas outlet, air purifier's the end of giving vent to anger is connected with the air inlet, air purifier's top fixed mounting carbon dioxide gas cylinder, carbon dioxide gas cylinder's the end of giving vent to anger is connected with the air inlet.

Preferably, place the platform and place the platform for circular, a plurality of observation holes evenly set up along placing platform circumference.

Preferably, clamping device is symmetrical structure, and half left clamping device includes the mount pad, and the mount pad is inside to be equipped with the cavity, and mount pad top and right-hand member are equipped with the through-hole, are equipped with the dead lever in the mount pad, and the dead lever is T type pole, dead lever left end fixed mounting compression spring, and mount pad top and right-hand member through-hole are passed respectively at the other both ends of dead lever, dead lever right-hand member fixed mounting clamp splice, and the clamp splice is the arc clamp splice.

Preferably, the culture dish comprises culture dish body and culture dish lid, sets up in the middle of the culture dish lid has the gas pocket, and the slot has been seted up to the gas pocket lateral wall, and the interpolation of slot connects the shutoff piece.

Preferably, the detector comprises a temperature detector, a gas concentration detector and a humidity detector.

Preferably, the water outlet end of the water purifier is provided with a control valve, and the air outlet end of the carbon dioxide gas cylinder is provided with an electromagnetic valve.

Preferably, the incubator front side is equipped with the chamber door, fixed mounting display and PLC controller on the chamber door, display and camera electric connection, PLC controller respectively with motor, heating pipe, detector, LED lamp plate, air purifier and solenoid valve electric connection.

Compared with the prior art, the utility model has the advantages that:

the utility model discloses a rotatory platform of placing and place and set up the observation hole on the platform, cooperate and place the platform below and set up the camera and can observe and monitor the culture dish, compare with traditional culture apparatus the camera setting in the culture dish below, can avoid because of culture dish cover play water smoke cause can't observe the culture dish inside clearly clear; the air hole is formed in the middle of the culture dish cover, so that a required gas environment can be provided for the culture in the culture dish, dust can be prevented from falling into the culture dish, and water can be added without uncovering the culture dish cover by matching with the rotation of the placing table; an air purifier and a carbon dioxide gas cylinder are arranged at the top of the incubator, and the air purifier and the carbon dioxide gas cylinder are both connected with an air inlet, so that the air in the incubator can be purified and circulated, and a carbon dioxide source is provided; the LED lamp plate can provide the illumination of different intensity and wavelength for incubator inside, conveniently studies the influence of illumination to cell culture.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the present invention;

fig. 3 is a schematic structural view of the clamping device of the present invention;

fig. 4 is a schematic structural view of the culture dish of the present invention.

In the figure:

1-incubator, 2-placing table, 3-observation hole, 4a, 4 b-clamping device, 5-culture dish, 6-motor, 7-camera, 8-water purifier, 9-liquid feeding pipe, 10-heating pipe, 11-detector, 12-LED lamp panel, 13-air purifier, 14-air inlet, 15-air outlet, 16-carbon dioxide gas bottle, 17-box door, 18-display, 19-PLC controller, 401-mounting seat, 402-fixing rod, 403-compression spring, 404-clamping block, 501-culture dish body, 502-culture dish cover, 503-air hole, 504-blocking piece.

Detailed Description

In order to better enable those skilled in the art to understand the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

As shown in FIGS. 1-4, a sample culture apparatus for cell biology experiments comprises an incubator 1, a placing table 2 is arranged at the bottom of the incubator 1, a plurality of observation holes 3 are arranged on the placing table 2, an organic glass plate is fixedly arranged in the observation holes 3,

clamping devices

4a and 4b are fixedly arranged at two sides above the observation holes 3, culture dishes 5 are arranged in the

clamping devices

4a and 4b, the bottoms of the culture dishes 5 are matched with the observation holes 3, the bottom of the placing table 2 is rotatably connected with the bottom of the incubator 1 through a rotating shaft, a motor 6 is fixedly arranged in the middle of the bottom of the incubator 1, an output shaft of the motor 6 is fixedly connected with the rotating shaft of the placing table 2, a camera 7 is fixedly arranged at the left side of the bottom of the incubator 1, the camera 7 is positioned under the observation holes 3, a water purifier 8 is fixedly arranged on the outer wall of the incubator 1, a water outlet end of the water purifier 8 is, 9 left ends of filling tube are equipped with filling opening 901, filling opening 901 position and culture dish 5 are directly over, 1 left side inner wall fixed mounting heating pipe 10 of incubator, fixed mounting detector 11 in the middle of the 1 top inner wall of incubator, 1 top inner wall fixed mounting LED lamp plate 12 of incubator of detector 11 both sides, 1 top outer wall fixed mounting air purifier 13 of incubator, 1 rear side inner wall of incubator is equipped with a plurality of

air inlets

14, 1 top both sides of incubator are equipped with gas outlet 15, air purifier 13's inlet end is connected with gas outlet 15, air purifier 13's the end of giving vent to anger is connected with air inlet 14, air purifier 13's top fixed mounting carbon dioxide gas cylinder 16, carbon dioxide gas cylinder 16's the end of giving vent to anger is connected with air inlet 14.

Place the platform 2 and evenly set up along placing 2 circumference of platform for circular platform of placing, a plurality of observation holes 3.

Clamping

device

4a, 4b are symmetrical structure, and left half clamping device 4a includes mount pad 401, and the inside cavity that is equipped with of mount pad 401, mount pad 401 top and right-hand member are equipped with the through-hole, are equipped with dead lever 402 in the mount pad 401, and dead lever 402 is T type pole, dead lever 402 left end fixed mounting compression spring 403, and mount pad 401 top and right-hand member through-hole are passed respectively at dead lever 402 other both ends, dead lever 402 right-hand member fixed mounting clamp splice 404, and clamp splice 404 is the arc clamp splice.

The culture dish 5 is composed of a culture dish body 501 and a culture dish cover 502, wherein the middle of the culture dish cover 502 is provided with an air hole 503, the side wall of the air hole 503 is provided with a slot, and a plugging sheet 504 is inserted in the slot.

The detector 11 includes a temperature detector, a gas concentration detector, and a humidity detector.

The water outlet end of the water purifier 8 is provided with a control valve 801, and the air outlet end of the carbon dioxide gas cylinder 16 is provided with an electromagnetic valve 1601.

The incubator 1 front side is equipped with chamber door 17, and fixed mounting display 18 and PLC controller 19 on the chamber door 17, display 18 and camera 7 electric connection, PLC controller respectively with motor 6, heating pipe 10, detector 11, LED lamp plate 12, air purifier 12 and solenoid valve 1601 electric connection.

The working principle is as follows:

after the culture medium is prepared, the culture medium is placed into the culture dish 5, the culture dish cover 502 is covered after the cells to be cultured are inoculated, the plugging sheet 504 is pulled out, the culture dish 5 is placed into the

clamping devices

4a and 4b according to the number, the

clamping devices

4a and 4b are fixed, the bottom of the culture dish 5 is opposite to the observation hole 3, the box door 17 is closed, the temperature and the carbon dioxide concentration of culture are set through the PLC, when the temperature is lower, the PLC controls the heating pipe 10 to be opened and heated, when the carbon dioxide concentration is lower, the PLC controls the electromagnetic valve 1601 to be opened and the carbon dioxide is injected, when the moisture in the culture dish 5 is lower, the control valve 801 is manually opened to open and the injected water, the purified water drips through the liquid filling opening 901 of the liquid filling pipe 9 and falls into the culture dish after passing through the air hole 503 of the culture dish.

Place the platform 2 bottom and rotate through pivot and 1 bottoms of incubator and be connected, fixed mounting motor 6 in the middle of 1 bottoms of incubator, the output shaft of motor 6 with place platform 2's pivot fixed connection, 1 bottoms of incubator left side fixed mounting camera 7, camera 7 is located under observation hole 3. The motor 6 rotates and can drive and place platform 2 and rotate for place culture dish 5 on the platform 2 and can rotate, can observe and monitor culture dish 5 above the observation hole 3 when the observation hole 3 rotates directly over the camera 7, and on the same way, can add water to culture dish 5 when culture dish 5 rotates under to filling opening 901.

In the description herein, references to the description of "one embodiment," "an example," "a specific example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not exhaustive and do not limit the specific implementations of the invention. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A cell biology experiment sample culture equipment is characterized in that: comprises an incubator (1), a placing platform (2) is arranged at the bottom of the incubator (1), a plurality of observing holes (3) are arranged on the placing platform (2), organic glass plates are fixedly installed in the observing holes (3), clamping devices (4 a, 4 b) are fixedly installed at two sides above the observing holes (3), culture dishes (5) are arranged in the clamping devices (4 a, 4 b), the bottoms of the culture dishes (5) are matched with the observing holes (3), the bottom of the placing platform (2) is rotatably connected with the bottom of the incubator (1) through a rotating shaft, a motor (6) is fixedly installed in the middle of the bottom of the incubator (1), an output shaft of the motor (6) is fixedly connected with the rotating shaft of the placing platform (2), a camera (7) is fixedly installed at the left side of the bottom of the incubator (1), the camera (7) is positioned under the observing holes (3), and a water purifier (8) is fixedly installed on the outer wall of the incubator (, the water outlet end of the water purifier (8) is connected with a liquid feeding pipe (9), the liquid feeding pipe is fixedly installed on the inner wall of the right side of the incubator (1), a liquid feeding port (901) is formed in the left end of the liquid feeding pipe (9), the liquid feeding port (901) is located right above the culture dish (5), a heating pipe (10) is fixedly installed on the inner wall of the left side of the incubator (1), a detector (11) is fixedly installed in the middle of the inner wall of the top of the incubator (1), LED lamp panels (12) are fixedly installed on the inner wall of the top of the incubator (1) on two sides of the detector (11), an air purifier (13) is fixedly installed on the outer wall of the top of the incubator (1), a plurality of air inlets (14) are formed in the inner wall of the rear side of the incubator (1), air outlets (15) are formed in two sides of the top of the incubator (1), an air inlet end, the top end of the air purifier (13) is fixedly provided with a carbon dioxide gas cylinder (16), and the air outlet end of the carbon dioxide gas cylinder (16) is connected with the air inlet (14).

2. The sample culture apparatus for cell biology experiment according to claim 1, wherein: the placing table (2) is a circular placing table, and a plurality of observation holes (3) are uniformly formed along the circumferential direction of the placing table (2).

3. The sample culture apparatus for cell biology experiment according to claim 1, wherein: clamping device (4 a, 4 b) are symmetrical structure, and half left clamping device (4 a) includes mount pad (401), mount pad (401) inside is equipped with the cavity, and mount pad (401) top and right-hand member are equipped with the through-hole, be equipped with dead lever (402) in mount pad (401), dead lever (402) are T type pole, dead lever (402) left end fixed mounting compression spring (403), and mount pad (401) top and right-hand member through-hole are passed respectively at dead lever (402) other both ends, dead lever (402) right-hand member fixed mounting clamp splice (404), clamp splice (404) are the arc clamp splice.

4. The sample culture apparatus for cell biology experiment according to claim 1, wherein: the culture dish (5) comprises culture dish body (501) and culture dish lid (502), and air hole (503) have been seted up in the middle of culture dish lid (502), slot has been seted up to air hole (503) lateral wall, plug piece (504) are inserted to connect in the slot.

5. The sample culture apparatus for cell biology experiment according to claim 1, wherein: the detector (11) comprises a temperature detector, a gas concentration detector and a humidity detector.

6. The sample culture apparatus for cell biology experiment according to claim 1, wherein: the water outlet end of the water purifier (8) is provided with a control valve (801), and the air outlet end of the carbon dioxide gas bottle (16) is provided with an electromagnetic valve (1601).

7. The sample culture apparatus for cell biology experiment according to claim 1, wherein: incubator (1) front side is equipped with chamber door (17), fixed mounting display (18) and PLC controller (19) are gone up in chamber door (17), display (18) and camera (7) electric connection, PLC controller respectively with motor (6), heating pipe (10), detector (11), LED lamp plate (12), air purifier (13) and solenoid valve (1601) electric connection.