CN111733075B

CN111733075B - Stem cell culture device

Info

Publication number: CN111733075B
Application number: CN202010632537.5A
Authority: CN
Inventors: 陈飞; 杨方园
Original assignee: Shanghai Zhongcheng Nuozhou Biotechnology Co ltd
Current assignee: Hengfeng Mingcheng Biotechnology Shanghai Co ltd
Priority date: 2020-07-03
Filing date: 2020-07-03
Publication date: 2023-03-14
Anticipated expiration: 2040-07-03
Also published as: CN111733075A

Abstract

The invention relates to a stem cell culture device in the technical field of stem cell culture, which comprises a box body, wherein the box body is provided with a liquid storage tank, a liquid pump and a liquid outlet pipe, the liquid storage tank is connected with the liquid pump, the liquid pump is provided with the liquid outlet pipe, the liquid outlet pipe is provided with a transverse pipe, the transverse pipe is provided with a spray head, the box body is provided with a rotating motor, the rotating motor is provided with a rotary disc, the rotary disc is provided with a jack, the jack is matched with the spray head, a limiting structure is arranged on the jack, the box body is provided with an air inlet pipe and an air outlet pipe, the air inlet pipe is provided with a sleeve, the sleeve is provided with a movable plate, the sleeve is provided with an adsorbent, the air inlet pipe is provided with a mounting frame, the mounting frame is provided with a draught fan, the air outlet pipe is provided with a one-way control structure, the box body is provided with a door plate, the door plate is provided with a sealing gasket for sealing, a glass window for observation is fixedly arranged in a through groove of the door plate.

Description

Stem cell culture device

Technical Field

The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device.

Background

Stem cells are a class of cells that have unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells.

The definition of stem cells has been revised and defined at different levels over the years. Most biologists and physicians now believe that stem cells are a class of cells derived from embryos, fetuses, or adults that have the ability to self-renew and proliferate and differentiate without restriction under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, and also capable of producing specialized cells that make up body tissues and organs, and also capable of differentiating into progenitor cells.

The culture vessel is a laboratory vessel for the cultivation of microorganisms or cells. A heater and a humidity regulator are installed in a conventional stem cell culture container. The existing stem cell incubator has the following problems in the use process: 1) The culture dish is driven to rotate by the motor arranged in the box body, so that stem cells at each position in the culture dish are heated more uniformly, but in the rotating process of the culture dish, the culture dish is only placed in the placing groove, so that the phenomenon that the culture dish moves in the placing groove is easily caused, and the culture dish cannot be effectively fixed; 2) The culture solution is inconvenient to add when the culture dish rotates, needs the manual work to open the device just can add the culture solution.

Disclosure of Invention

Solves the technical problem

In view of the above-mentioned disadvantages of the prior art, the present invention provides a stem cell culture apparatus.

Technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme:

the utility model provides a stem cell culture device, includes the box, the top fixed mounting of box has liquid reserve tank, liquid pump and drain pipe, the liquid reserve tank passes through the input fixed connection of pipeline and liquid pump, the output fixedly connected with drain pipe of liquid pump, the bottom fixedly connected with of drain pipe violently manages, the even fixedly connected with shower nozzle in bottom of violently managing, the interior bottom fixed mounting of box has the rotation motor, the output fixedly connected with carousel of rotation motor, the top of carousel is along the even fixedly connected with jack of circumferencial direction, the mounted position of seting up the position of jack and shower nozzle cooperatees, the inner wall symmetry fixedly connected with of jack is used for the spacing limit structure of culture dish, lateral wall through-hole department difference fixedly connected with intake pipe and outlet duct about the box, the left end threaded connection of intake pipe has the sleeve pipe, sheathed tube inner wall threaded connection has the fly leaf, the through-hole that is used for the air flow is evenly offered to the sheathed tube left end and fly leaf, the sleeve pipe is equipped with the adsorbent that is used for the absorption in the left side department of fly leaf, fixed mounting bracket has the draught fan that is used for drawing the air flow, be equipped with the one-way control hasp in the fixed control structure of door plant, the door plant is used for the sealed glass to observe the sealed inslot of hinge.

Furthermore, limit structure includes movable block, cross slot and second spring, the cross slot has been seted up to the lateral wall horizontal symmetry of jack, the inner end fixedly connected with second spring of cross slot, the outer end fixedly connected with movable block of second spring, movable block and cross slot laminating sliding connection.

Furthermore, the movable block is made of rubber materials.

Furthermore, the one-way control structure includes ring, arc wall, hemisphere piece, first spring and straight board, the straight board of outer end fixedly connected with of outlet duct, the first spring of left side wall fixedly connected with of straight board, the left end fixedly connected with hemisphere piece of first spring, the arc wall that uses with the cooperation of hemisphere piece is seted up to the ring.

Furthermore, the hemisphere block is fixedly connected with the inner wall of the air outlet pipe.

Furthermore, the rotating motor and the mounting rack are both electrically connected with an external power supply.

Advantageous effects

Compared with the known public technology, the technical scheme provided by the invention has the following beneficial effects:

1. according to the invention, the culture dish is inserted into the jack, the second spring of the limiting structure pushes the movable block to abut against the culture dish, and the culture dish is limited in the jack, so that the stability of the culture dish is ensured, and the stability of stem cell culture is ensured.

2. According to the invention, the rotating motor drives the rotating disc to rotate, the rotating disc drives the whole row of insertion holes to move to the position right below the transverse pipe, then the liquid pump pumps culture solution in the liquid storage tank to enter the liquid outlet pipe, then the culture solution enters the transverse pipe, and finally the culture solution is sprayed out from the spray head, so that the culture solution is added to the whole row of culture dishes, the culture solution is convenient to add when the culture dishes rotate, and the device is not required to be manually opened when the culture solution is added, so that the device is convenient for practical use.

3. According to the invention, the sleeve is connected on the left side of the air inlet pipe, and the adsorbent between the sleeve and the movable plate is convenient to adsorb dust and bacteria in the air, so that clean air is ensured to enter the box body, the influence of the dust and the bacteria on stem cells is effectively avoided, meanwhile, the hemisphere block of the one-way control structure is pushed by aerodynamic force to move rightwards to separate from the arc-shaped groove, and redundant air is discharged outwards through the arc-shaped groove, so that the air replacement of the box body is facilitated.

4. According to the invention, the glass window is designed on the door plate, so that the specific situation of the box body can be conveniently observed by a worker.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

FIG. 1 is a schematic view of the main structure of the present invention;

FIG. 2 is a structural cross-sectional view of the present invention;

FIG. 3 is an enlarged view of the structure at A in FIG. 2 according to the present invention;

FIG. 4 is a schematic view of the turntable and its connection structure of the present invention;

the reference numerals in the drawings represent: 1. the box body 2, the air inlet pipe 3, the sleeve 4, the jack 5, the liquid storage box 6, the liquid pump 7, the liquid outlet pipe 8, the transverse pipe 9, the glass window 10, the air outlet pipe 11, the spray head 12, the rotary disc 13, the rotary motor 14, the door plate 15, the movable block 16, the mounting frame 17, the induced draft fan 18, the through hole 19, the movable plate 20, the circular ring 21, the arc-shaped groove 22, the semi-spherical block 23, the first spring 24, the straight plate 25, the transverse groove 26 and the second spring.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The present invention will be further described with reference to the following examples.

Examples

Referring to fig. 1-4, a stem cell culture apparatus of this embodiment includes a case 1, a liquid storage tank 5, a liquid pump 6 and a liquid outlet pipe 7 are fixedly mounted on a top of the case 1, the liquid storage tank 5 is fixedly connected to an input end of the liquid pump 6 through a pipeline, an output end of the liquid pump 6 is fixedly connected to the liquid outlet pipe 7, a horizontal pipe 8 is fixedly connected to a bottom of the liquid outlet pipe 7, a nozzle 11 is uniformly and fixedly connected to a bottom of the horizontal pipe 8, a rotating motor 13 is fixedly mounted on an inner bottom of the case 1, a rotating disc 12 is fixedly connected to an output end of the rotating motor 13, a plug hole 4 is uniformly and fixedly connected to a top of the rotating disc 12 along a circumferential direction, a plug hole 4 is formed at a position matched with a mounting position of the nozzle 11, the rotating disc 12 is driven to rotate by the rotating motor 13, the rotating disc 12 drives a whole row of plug holes 4 to move to a position under the horizontal pipe 8, then the liquid pump 6 pumps the culture liquid in the liquid storage tank 5 to enter the liquid outlet pipe 7 and then enter the horizontal pipe 8, and finally to be ejected from the nozzle 11, so as to add culture liquid to the culture dish, the culture dish can be conveniently added, the culture dish can be added without manual culture liquid without opening the apparatus;

the inner wall of the jack 4 is symmetrically and fixedly connected with a limiting structure used for limiting a culture dish, the limiting structure comprises a movable block 15, a transverse groove 25 and a second spring 26, the transverse groove 25 is horizontally and symmetrically formed in the side wall of the jack 4, the second spring 26 is fixedly connected to the inner end of the transverse groove 25, the movable block 15 is fixedly connected to the outer end of the second spring 26, the movable block 15 is in fit sliding connection with the transverse groove 25, the movable block 15 is made of rubber materials, the culture dish is inserted into the jack 4, the second spring 26 of the limiting structure pushes the movable block 15 to abut against the culture dish, the culture dish is limited in the jack 4, the stability of the culture dish is guaranteed, and the stability of dry cell culture is guaranteed;

an air inlet pipe 2 and an air outlet pipe 10 are fixedly connected to through holes of the left side wall and the right side wall of a box body 1 respectively, a sleeve 3 is in threaded connection with the left end of the air inlet pipe 2, a movable plate 19 is in threaded connection with the inner wall of the sleeve 3, through holes 18 for air flowing are uniformly formed in the left end of the sleeve 3 and the movable plate 19, an adsorbent for adsorption is filled in the left side of the movable plate 19 of the sleeve 3, an installation frame 16 is fixedly installed in the air inlet pipe 2, an induced draft fan 17 for pumping air flowing is fixedly installed on the installation frame 16, a one-way control structure for controlling air flowing in one way is arranged in the air outlet pipe 10, the one-way control structure comprises a circular ring 20, an arc-shaped groove 21, a hemispherical block 22, a first spring 23 and a straight plate 24, a straight plate 24 is fixedly connected to the outer end of the air outlet pipe 10, the first spring 23 is fixedly connected to the left side wall of the straight plate 24, and the hemispherical block 22 is fixedly connected to the left end of the first spring 23, the circular ring 20 is provided with an arc-shaped groove 21 matched with the hemispherical block 22 for use, the hemispherical block 22 is fixedly connected with the inner wall of the air outlet pipe 10, the left side of the air inlet pipe 2 is connected with the sleeve 3, an adsorbent between the sleeve 3 and the movable plate 19 facilitates adsorption treatment of dust and bacteria in the air, clean air is guaranteed to enter the box body 1, and influence of the dust and the bacteria on stem cells is effectively avoided;

the front side wall of the box body 1 is connected with a door plate 14 through a hinge and a lock catch, the inner wall of the door plate 14 is fixed with a sealing gasket for sealing, a glass window 9 for observation is fixedly arranged in a through groove of the door plate 14, and the glass window 9 is designed on the door plate 14, so that a worker can conveniently observe the specific conditions of the box body 1.

The rotating motor 13 and the mounting frame 16 are both electrically connected with an external power supply.

When the culture dish is used, as shown in fig. 1-4, the culture dish is inserted into the jack 4, the second spring 26 of the limiting structure pushes the movable block 15 to abut against the culture dish, the culture dish is limited in the jack 4, the stability of the culture dish is guaranteed, the stability of stem cell culture is guaranteed, when shaking is needed, the rotating motor 13 is started, the rotating motor 13 drives the rotating disc 12 to rotate, the rotating disc 12 drives the culture dish to rotate and shake evenly, when culture liquid needs to be added, the rotating disc 12 drives the whole row of jacks 4 to move to be right below the transverse pipe 8, then the liquid pump 6 pumps the culture liquid in the liquid storage tank 5 to enter the liquid outlet pipe 7 and then enter the transverse pipe 8, and finally the culture liquid is sprayed out of the spray head 11, so that the culture liquid is added to the whole row of culture dishes, the culture liquid is convenient to be added when the culture dish rotates, and the device does not need to be opened manually when the culture liquid is added, so that the culture liquid is convenient to be used practically; when needing to change the interior air of box 1, start draught fan 17, draught fan 17 introduces new air to box 1 through-hole 18, the convenient dust and the bacterium to the air of adsorbent between sleeve pipe 3 and the fly leaf 19 adsorb the processing, clean air gets into box 1 has been guaranteed, the influence of dust and bacterium to the stem cell has been avoided effectively, and simultaneously, the hemisphere piece 22 of aerodynamic promotion one-way control structure moves rightwards and breaks away from arc 21, unnecessary air passes through arc 21 and outwards discharges, be convenient for carry out the air change to box 1, furthermore, when need not to add the air, first spring 23 promotes hemisphere piece 22 and plugs up ring 20's arc 21, hemisphere piece 22 conveniently plugs up outlet duct 10 when not adding the air, avoid the outside air to get into box 1.

Wherein, design glass window 9 on the door plant 14, make things convenient for the staff to observe the particular case of box 1.

The above examples are only intended to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A stem cell culture device, includes box (1), its characterized in that: the utility model discloses a chemical biological adsorption device, including box (1), liquid pump (6), drain pipe (7), liquid storage tank (5), liquid pump (6) and liquid storage tank (5) are fixed mounting at the top of box (1), the input fixed connection of liquid pump (6) is passed through to liquid storage tank (5), the output fixedly connected with drain pipe (7) of liquid pump (6), the bottom fixedly connected with of drain pipe (7) violently manages (8), the even fixedly connected with shower nozzle (11) in bottom of violently managing (8), the interior bottom fixed mounting of box (1) has rotation motor (13), the output fixedly connected with carousel (12) of rotation motor (13), the top of carousel (12) is along the even fixedly connected with jack (4) of circumferencial direction, the mounted position of jack (4) cooperatees with the mounted position of shower nozzle (11), the inner wall symmetry fixedly connected with of jack (4) is used for the spacing limit structure of culture dish, the left and right side wall through-hole department of box (1) is fixedly connected with intake pipe (2) and outlet duct (10) respectively, the left end screw thread connection of intake pipe (2) has sleeve pipe (3), the inner wall screw thread connection of sleeve (3) has suction, the left side of fly leaf sleeve (19) and the left side filler of fly leaf (19) has the through-hole department to be used for the even through-hole (18) be used for the suction sleeve (19), the suction tube (3), the suction sleeve (19) is located to adsorb the flow at the left side of fly leaf (19), the air inlet pipe (2) is internally and fixedly provided with a mounting frame (16), the mounting frame (16) is fixedly provided with an induced draft fan (17) for pumping air to flow, a one-way control structure for controlling air to flow in one way is arranged in the air outlet pipe (10), the front side wall of the box body (1) is connected with a door plate (14) through a hinge and a lock catch, a sealing gasket for sealing is fixed on the inner wall of the door plate (14), and a glass window (9) for observation is fixedly arranged in a through groove of the door plate (14); the limiting structure comprises a movable block (15), a transverse groove (25) and a second spring (26), the transverse groove (25) is horizontally and symmetrically formed in the side wall of the insertion hole (4), the second spring (26) is fixedly connected to the inner end of the transverse groove (25), the movable block (15) is fixedly connected to the outer end of the second spring (26), and the movable block (15) is attached to the transverse groove (25) in a sliding mode; the unidirectional control structure includes ring (20), arc wall (21), hemisphere piece (22), first spring (23) and straight board (24), straight board (24) of outer end fixedly connected with of outlet duct (10), the first spring (23) of left side wall fixedly connected with of straight board (24), the left end fixedly connected with hemisphere piece (22) of first spring (23), arc wall (21) that cooperate the use with hemisphere piece (22) are seted up in ring (20).

2. A stem cell culture device according to claim 1, wherein the movable block (15) is made of rubber material.

3. A stem cell culture apparatus according to claim 1, wherein the hemispherical block (22) is fixedly connected to the inner wall of the outlet duct (10).

4. The stem cell culture apparatus of claim 1, wherein the rotating motor (13) and the mounting bracket (16) are electrically connected to an external power source.